WO2012177601A3 - Microorganisms for producing isobutanol and methods related thereto - Google Patents

Microorganisms for producing isobutanol and methods related thereto Download PDF

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Publication number
WO2012177601A3
WO2012177601A3 PCT/US2012/043091 US2012043091W WO2012177601A3 WO 2012177601 A3 WO2012177601 A3 WO 2012177601A3 US 2012043091 W US2012043091 W US 2012043091W WO 2012177601 A3 WO2012177601 A3 WO 2012177601A3
Authority
WO
WIPO (PCT)
Prior art keywords
coa
butanol
pathway
naturally occurring
isobutanol
Prior art date
Application number
PCT/US2012/043091
Other languages
French (fr)
Other versions
WO2012177601A2 (en
Inventor
Anthony P. Burgard
Robin E. Osterhout
Jun Sun
Priti Pharkya
Original Assignee
Genomatica, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomatica, Inc. filed Critical Genomatica, Inc.
Publication of WO2012177601A2 publication Critical patent/WO2012177601A2/en
Publication of WO2012177601A3 publication Critical patent/WO2012177601A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

Provided herein is a non-naturally occurring microbial organism having an isopropanol pathway and comprising at least one exogenous nucleic acid encoding an isopropanol pathway enzyme. In certain embodiments, the pathway includes an enzyme selected from a 4-hydroxybutyryl-CoA dehydratase, a crotonase, a 3-hydroxybutyryl-CoA dehydrogenase, an acetoacetyl-CoA synthetase, an acetyl-CoA:acetoacetate-CoA transferase, an acetoacetyl-CoA hydrolase, an acetoacetate decarboxylase, and an acetone reductase. Also provided herein is anon-naturally occurring microbial organism having an n-butanol pathway and comprising at least one exogenous nucleic acid encoding an n-butanol pathway enzyme. Other non-naturally occurring microbial organism having n-butanol or isobutanol pathways are also provided herein. In certain embodiments, isobutanol pathways utilizing reverse TCA and/or reducing equivalents from CO and/or hydrogen are used to enhance product yields. The organisms provided herein can be cultured to produce isopropanol, n-butanol, or isobutanol.
PCT/US2012/043091 2011-06-22 2012-06-19 Microorganisms for producing isobutanol and methods related thereto WO2012177601A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161500124P 2011-06-22 2011-06-22
US61/500,124 2011-06-22
US201161502708P 2011-06-29 2011-06-29
US61/502,708 2011-06-29

Publications (2)

Publication Number Publication Date
WO2012177601A2 WO2012177601A2 (en) 2012-12-27
WO2012177601A3 true WO2012177601A3 (en) 2014-05-08

Family

ID=47423164

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/043091 WO2012177601A2 (en) 2011-06-22 2012-06-19 Microorganisms for producing isobutanol and methods related thereto

Country Status (1)

Country Link
WO (1) WO2012177601A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5803563B2 (en) * 2011-10-24 2015-11-04 トヨタ自動車株式会社 Method for producing isobutanol
JP2016165225A (en) 2013-07-09 2016-09-15 味の素株式会社 Method for producing useful substance
CN107404882A (en) 2015-02-27 2017-11-28 白狗实验室有限公司 For producing acetone, isopropanol, butyric acid, other biological product and its mixotrophism fermentation process of mixture
CN107354178B (en) * 2017-07-20 2021-06-11 盐城工学院 Method for promoting microorganisms to synthesize 2, 3-butanediol by adding amino acid
CN115851563B (en) * 2022-10-13 2023-10-20 深圳中科翎碳生物科技有限公司 Engineering bacterium for electrosynthesis of 3-hydroxybutyric acid by fixing carbon dioxide and construction method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100304453A1 (en) * 2008-12-16 2010-12-02 Genomatica, Inc. Microorganisms and methods for conversion of syngas and other carbon sources to useful products
US20100323418A1 (en) * 2009-04-30 2010-12-23 Genomatica, Inc. ORGANISMS FOR THE PRODUCTION OF ISOPROPANOL, n-BUTANOL, AND ISOBUTANOL
US20110129899A1 (en) * 2009-10-13 2011-06-02 Robert Haselbeck Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-coa, putrescine and related compounds, and methods related thereto

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100304453A1 (en) * 2008-12-16 2010-12-02 Genomatica, Inc. Microorganisms and methods for conversion of syngas and other carbon sources to useful products
US20100323418A1 (en) * 2009-04-30 2010-12-23 Genomatica, Inc. ORGANISMS FOR THE PRODUCTION OF ISOPROPANOL, n-BUTANOL, AND ISOBUTANOL
US20110129899A1 (en) * 2009-10-13 2011-06-02 Robert Haselbeck Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-coa, putrescine and related compounds, and methods related thereto

Also Published As

Publication number Publication date
WO2012177601A2 (en) 2012-12-27

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