WO2014004616A3 - Engineered yeast with improved growth under low aeration - Google Patents

Engineered yeast with improved growth under low aeration Download PDF

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Publication number
WO2014004616A3
WO2014004616A3 PCT/US2013/047789 US2013047789W WO2014004616A3 WO 2014004616 A3 WO2014004616 A3 WO 2014004616A3 US 2013047789 W US2013047789 W US 2013047789W WO 2014004616 A3 WO2014004616 A3 WO 2014004616A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombinant yeast
pyruvate
engineered
under low
growth under
Prior art date
Application number
PCT/US2013/047789
Other languages
French (fr)
Other versions
WO2014004616A2 (en
Inventor
Catherine Asleson Dundon
Thomas Buelter
Kevin Roberg-Perez
Original Assignee
Gevo, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gevo, Inc. filed Critical Gevo, Inc.
Publication of WO2014004616A2 publication Critical patent/WO2014004616A2/en
Publication of WO2014004616A3 publication Critical patent/WO2014004616A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01001—Pyruvate decarboxylase (4.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/99—Oxidoreductases acting on NADH or NADPH (1.6) with other acceptors (1.6.99)
    • C12Y106/99003—NADH dehydrogenase (1.6.99.3)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

The present application relates to recombinant yeast microorganisms comprising biosynthetic pathways and methods of using said recombinant yeast microorganisms to produce various beneficial pyruvate-derived metabolites, e.g., isobutanol. In some embodiments, the recombinant yeast microorganisms may comprise a metabolic pathway for the production of a pyruvate-derived metabolite, wherein said metabolic pathway comprises at least one exogenous gene and/or at least one overexpressed endogenous gene encoding an enzyme that catalyzes a pathway step in the production of the pyruvate-derived metabolite, wherein said recombinant yeast microorganism is engineered to comprise reduced pyruvate decarboxylase (PDC) activity, and wherein said recombinant yeast microorganism is engineered to reduce or eliminate the expression or activity of an endogenous polypeptide encoded by a gene selected from NDE1 and NDE2.
PCT/US2013/047789 2012-06-26 2013-06-26 Engineered yeast with improved growth under low aeration WO2014004616A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261664267P 2012-06-26 2012-06-26
US61/664,267 2012-06-26

Publications (2)

Publication Number Publication Date
WO2014004616A2 WO2014004616A2 (en) 2014-01-03
WO2014004616A3 true WO2014004616A3 (en) 2014-02-20

Family

ID=49784003

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/047789 WO2014004616A2 (en) 2012-06-26 2013-06-26 Engineered yeast with improved growth under low aeration

Country Status (1)

Country Link
WO (1) WO2014004616A2 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9556443B2 (en) * 2013-07-19 2017-01-31 Samsung Electronics Co., Ltd. Yeast cell with inactivated NADH dehydrogenase and method of producing lactate using the yeast cell
BR112017024689B1 (en) 2015-06-17 2022-04-12 Clariant International Ltd Water-soluble or water-swellable polymers, their production process, their use, process of cementing deep drilling using a cement slurry and polymeric mixture
FI3394251T3 (en) * 2015-12-23 2023-03-23 Technip Energies France Host cells for dicarboxylic acid production
EP3472128B1 (en) 2016-06-20 2021-12-22 Clariant International Ltd Compound comprising certain level of bio-based carbon
JP7050784B2 (en) 2016-12-12 2022-04-08 クラリアント・インターナシヨナル・リミテツド Use of bio-based polymers in cosmetic compositions, dermatological compositions or pharmaceutical compositions
US20200009041A1 (en) 2016-12-12 2020-01-09 Clariant International Ltd. Polymer Comprising Certain Level Of Bio-Based Carbon
EP3551681A1 (en) 2016-12-12 2019-10-16 Clariant International Ltd Polymer comprising certain level of bio-based carbon
BR112019011780B1 (en) 2016-12-12 2023-03-07 Clariant International Ltd POLYMER COMPRISING CARBON FROM BIOLOGICAL MATERIAL, ITS OBTAINMENT PROCESS AND ITS USE
EP3554645A1 (en) 2016-12-15 2019-10-23 Clariant International Ltd Water-soluble and/or water-swellable hybrid polymer
WO2018108667A1 (en) 2016-12-15 2018-06-21 Clariant International Ltd Water-soluble and/or water-swellable hybrid polymer
WO2018108664A1 (en) 2016-12-15 2018-06-21 Clariant International Ltd Water-soluble and/or water-swellable hybrid polymer
US11542343B2 (en) 2016-12-15 2023-01-03 Clariant International Ltd Water-soluble and/or water-swellable hybrid polymer
JP6879111B2 (en) 2017-08-02 2021-06-02 トヨタ自動車株式会社 Recombinant yeast and method for producing ethanol using it

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6723837B1 (en) * 1999-07-12 2004-04-20 Monsanto Technology Llc Nucleic acid molecule and encoded protein associated with sterol synthesis and metabolism
US7365164B2 (en) * 2003-03-04 2008-04-29 Suntory Limited Screening method for genes of brewing yeast
WO2009086423A2 (en) * 2007-12-23 2009-07-09 Gevo, Inc. Yeast organism producing isobutanol at a high yield
US20090275097A1 (en) * 2008-03-05 2009-11-05 Jun Sun Primary alcohol producing organisms

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6723837B1 (en) * 1999-07-12 2004-04-20 Monsanto Technology Llc Nucleic acid molecule and encoded protein associated with sterol synthesis and metabolism
US7365164B2 (en) * 2003-03-04 2008-04-29 Suntory Limited Screening method for genes of brewing yeast
WO2009086423A2 (en) * 2007-12-23 2009-07-09 Gevo, Inc. Yeast organism producing isobutanol at a high yield
US20090275097A1 (en) * 2008-03-05 2009-11-05 Jun Sun Primary alcohol producing organisms

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CAI ET AL.: "Genomewide Screen Reveals a Wide Regulatory Network for Di/Tripeptide Utilization in Saccharomyces cerevisiae.", GENETICS., vol. 172, 15 December 2005 (2005-12-15), pages 1459 - 1476 *
KERSCHER ET AL.: "A single external enzyme confers alternative NADH:ubiquinone oxidoreductase activity in Yarrowia lipolytica.", JOURNAL OF CELL SCIENCE., vol. 112, 24 June 1999 (1999-06-24), pages 2347 - 2352. *
KO ET AL.: "Control of 5-FOA and 5-FU resistance by Saccharomyces cerevisiae YJL055W.", YEAST., vol. 25, 9 January 2008 (2008-01-09), pages 155 - 160. *
STEPCHENKOVA ET AL.: "Genome-wide screening for genes whose deletions confer sensitivity to mutagenic purine base analogs in yeast.", BMC GENETICS., vol. 6, 2 June 2005 (2005-06-02), pages 1 - 11 *

Also Published As

Publication number Publication date
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