EA199901057A1 - Способ, основанный на применении бактериофагов, для обнаружения биологических молекул в биологических образцах - Google Patents

Способ, основанный на применении бактериофагов, для обнаружения биологических молекул в биологических образцах

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Publication number
EA199901057A1
EA199901057A1 EA199901057A EA199901057A EA199901057A1 EA 199901057 A1 EA199901057 A1 EA 199901057A1 EA 199901057 A EA199901057 A EA 199901057A EA 199901057 A EA199901057 A EA 199901057A EA 199901057 A1 EA199901057 A1 EA 199901057A1
Authority
EA
Eurasian Patent Office
Prior art keywords
phage
molecule
molecules
interest
expressed
Prior art date
Application number
EA199901057A
Other languages
English (en)
Inventor
Риккардо Кортезе
Паоло Моначи
Original Assignee
Иституто Ди Ричерке Ди Биолоджиа Молеколаре П.Анджелетти С.П.А.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Иституто Ди Ричерке Ди Биолоджиа Молеколаре П.Анджелетти С.П.А. filed Critical Иституто Ди Ричерке Ди Биолоджиа Молеколаре П.Анджелетти С.П.А.
Publication of EA199901057A1 publication Critical patent/EA199901057A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds

Abstract

Настоящее изобретение относится к способу, который позволяет применять нитчатые бактериофаги для определения молекул, представляющих интерес, в биологических образцах. Способ включает в себя ряд дополняющих друг друга стадий, которые зависят от характеристик применяемого фага. На капсиде фага экспрессируется, по крайней мере, одна молекула, обычно, но не обязательно, белковой природы, способная связываться в образце, по крайней мере, с одной из представляющих интерес молекул. В соответствии со способом по изобретению, вышеуказанную молекулу определяют, используя взаимосвязь между молекулой, экспрессируемой на поверхности фага и геномом фага. Способность образовывать специфичные комплексы, типичные для молекул, экспрессируемых на капсиде, предполагает образование комплексов между представляющей интерес молекулой и фагом, которые обнаруживают путем амплификации известных последовательностей ДНК фага. Способ по настоящему изобретению, имеет чрезвычайно высокую чувствительность и может использоваться, в частности, для диагностических и прогностических целей.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA199901057A 1997-05-22 1998-05-22 Способ, основанный на применении бактериофагов, для обнаружения биологических молекул в биологических образцах EA199901057A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT97RM000304A IT1291913B1 (it) 1997-05-22 1997-05-22 Metodo che prevede l'uso di batteriofagi per la rivelazione della presenza di molecole di interesse in campioni biologici
PCT/IT1998/000130 WO1998053100A1 (en) 1997-05-22 1998-05-22 Method based on the use of bacteriophages for the detection biological molecules in biological samples

Publications (1)

Publication Number Publication Date
EA199901057A1 true EA199901057A1 (ru) 2000-04-24

Family

ID=11405087

Family Applications (1)

Application Number Title Priority Date Filing Date
EA199901057A EA199901057A1 (ru) 1997-05-22 1998-05-22 Способ, основанный на применении бактериофагов, для обнаружения биологических молекул в биологических образцах

Country Status (15)

Country Link
US (1) US6265169B1 (ru)
EP (1) EP0983386B1 (ru)
JP (1) JP3143477B2 (ru)
CN (1) CN1261407A (ru)
AT (1) ATE286540T1 (ru)
AU (1) AU733215B2 (ru)
CA (1) CA2290740C (ru)
DE (1) DE69828502T2 (ru)
EA (1) EA199901057A1 (ru)
ES (1) ES2235329T3 (ru)
IL (1) IL132980A0 (ru)
IT (1) IT1291913B1 (ru)
NO (1) NO995700L (ru)
PT (1) PT983386E (ru)
WO (1) WO1998053100A1 (ru)

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US20020192676A1 (en) * 2001-06-18 2002-12-19 Madonna Angelo J. Method for determining if a type of bacteria is present in a mixture
DE10129815A1 (de) * 2001-06-24 2003-01-09 Profos Ag Verfahren zur Aufreinigung von Bakterienzellen und Zellbestandteilen
US8216780B2 (en) * 2002-04-12 2012-07-10 Microphage (Tm) Incorporated Method for enhanced sensitivity in bacteriophage-based diagnostic assays
US20050003346A1 (en) * 2002-04-12 2005-01-06 Colorado School Of Mines Apparatus and method for detecting microscopic living organisms using bacteriophage
AU2003228505B2 (en) * 2002-04-12 2009-01-08 Colorado School Of Mines Method for detecting low concentrations of a target bacterium that uses phages to infect target bacterial cells
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JP4991291B2 (ja) * 2003-06-20 2012-08-01 ディスカバーエクス コーポレイション タンパク質結合を検出するための検定法およびキット
US20080241819A1 (en) * 2006-10-31 2008-10-02 Microphage (Tm) Incorporated Method and apparatus for enhanced bacteriophage-based diagnostic assays by selective inhibition of potential cross-reactive organisms
US20050250096A1 (en) * 2004-02-13 2005-11-10 Microphage, Incorporated Microorganism detection using bacteriophage amplification
US20110097702A1 (en) * 2005-03-31 2011-04-28 Voorhees Kent J Methods and compositions for in situ detection of microorganisms on a surface
WO2006105504A1 (en) * 2005-03-31 2006-10-05 Microphage Incorporated Apparatus and method for detecting microorganisms using flagged bacteriophage
US20070178450A1 (en) * 2006-01-27 2007-08-02 Microphage (Tm) Incorporation Method and apparatus for determining level of microorganisms using bacteriophage
CN100386628C (zh) * 2006-04-21 2008-05-07 中国科学院武汉病毒研究所 噬菌体免疫pcr检测病原的方法
AU2008265989B8 (en) 2007-06-15 2012-01-12 Microphage Incorporated Method of detection of microorganisms with enhanced bacteriophage amplification
US8697434B2 (en) * 2008-01-11 2014-04-15 Colorado School Of Mines Detection of phage amplification by SERS nanoparticles
ES2325825B1 (es) * 2008-03-17 2010-06-25 Consejo Superior De Investigaciones Cientificas (Csic) (75%) Procedimiento y sistema para detectar y/o cuantificar bacteriofagos susceptibles de infectar una cepa huesped bacteriana predeterminada, uso de un dispositivo microelectronico sensor para detectar dichos bacteriofagos y dispositivo microelectronico sensor para llevar a cabo dicho.
US9441204B2 (en) * 2008-04-03 2016-09-13 Colorado School Of Mines Compositions and methods for detecting Yersinia pestis bacteria
WO2010123625A1 (en) 2009-04-24 2010-10-28 University Of Southern California Cd133 polymorphisms predict clinical outcome in patients with cancer
WO2011084757A1 (en) 2009-12-21 2011-07-14 University Of Southern California Germline polymorphisms in the sparc gene associated with clinical outcome in gastric cancer
WO2011085334A1 (en) 2010-01-11 2011-07-14 University Of Southern California Cd44 polymorphisms predict clinical outcome in patients with gastric cancer
JP2014063220A (ja) * 2012-09-19 2014-04-10 Sharp Corp 情報処理装置、情報処理装置の制御方法、制御プログラム、および記録媒体
US9433239B2 (en) 2013-03-15 2016-09-06 Altria Client Services Llc Bacteriophage and methods of making and using
US9781929B2 (en) 2015-01-29 2017-10-10 Altria Client Services Llc Bacteriophage and methods of using
WO2016123425A1 (en) 2015-01-29 2016-08-04 Altria Client Services Llc Endolysin from bacteriophage against geobacillus and methods of using
CN113265489B (zh) * 2021-06-01 2021-12-14 广州医科大学 一种基于ms2噬菌体研究蛋白相互作用的新方法

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JPS61501489A (ja) 1984-03-19 1986-07-24 ユニバ−シテイ　オブ　イリノイ 認識及び同定剤としてのバクテリオファ−ジ
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Also Published As

Publication number Publication date
WO1998053100A1 (en) 1998-11-26
JP3143477B2 (ja) 2001-03-07
DE69828502T2 (de) 2006-01-05
CA2290740C (en) 2004-12-14
AU7548098A (en) 1998-12-11
US6265169B1 (en) 2001-07-24
AU733215B2 (en) 2001-05-10
NO995700D0 (no) 1999-11-19
EP0983386A1 (en) 2000-03-08
ITRM970304A1 (it) 1998-11-22
CN1261407A (zh) 2000-07-26
JP2000513949A (ja) 2000-10-24
NO995700L (no) 2000-01-24
IL132980A0 (en) 2001-03-19
EP0983386B1 (en) 2005-01-05
CA2290740A1 (en) 1998-11-26
ES2235329T3 (es) 2005-07-01
ITRM970304A0 (ru) 1997-05-22
ATE286540T1 (de) 2005-01-15
IT1291913B1 (it) 1999-01-21
PT983386E (pt) 2005-04-29
DE69828502D1 (de) 2005-02-10

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