EA011878B1 - Респираторно-синцитиальный вирус с перекрестно компенсированным геномным дефицитом - Google Patents
Респираторно-синцитиальный вирус с перекрестно компенсированным геномным дефицитом Download PDFInfo
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- EA011878B1 EA011878B1 EA200601218A EA200601218A EA011878B1 EA 011878 B1 EA011878 B1 EA 011878B1 EA 200601218 A EA200601218 A EA 200601218A EA 200601218 A EA200601218 A EA 200601218A EA 011878 B1 EA011878 B1 EA 011878B1
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| WO2013133702A1 (en) | 2012-03-05 | 2013-09-12 | De Staat Der Nederlanden, Vert. Door De Minister Van Vws | Methods and compositions for stabilizing dried biological materials |
| CN113322264B (zh) | 2013-03-14 | 2024-05-31 | 爱默蕾大学 | 具有沉默突变的重组rsv、其相关疫苗和方法 |
| BR112015031541A2 (pt) | 2013-06-17 | 2017-07-25 | De Staat Der Nederlanden Vert Door De Mini Van Vws Miniie Van Volksgezondheid Welzijn En Sport | métodos para o impedimento da agregação de componentes virais |
| CN106659777A (zh) | 2014-06-13 | 2017-05-10 | 葛兰素史密丝克莱恩生物有限公司 | 免疫原性组合产品 |
| MX2018005462A (es) | 2015-10-29 | 2018-08-01 | Univ Emory | Composiciones inmunogénicas de rsv quimérico y métodos de uso. |
| US10626126B2 (en) * | 2016-04-08 | 2020-04-21 | Pulmocide Limited | Compounds |
| CN112746059A (zh) * | 2020-09-15 | 2021-05-04 | 清华大学 | 基于病毒结构蛋白遗传互补的冠状病毒细胞模型 |
| CN112501134B (zh) * | 2020-12-09 | 2023-01-20 | 贵州医科大学附属医院 | 一种人呼吸道合胞病毒毒株及其应用 |
| CN114990079B (zh) * | 2022-04-29 | 2023-07-04 | 广州医科大学附属第一医院(广州呼吸中心) | 治疗性缺陷干扰颗粒及其在制备用于防治rsv病毒感染的产品中的应用 |
Citations (3)
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| WO1996010400A1 (en) * | 1994-09-30 | 1996-04-11 | The Uab Research Foundation | Gene therapy vectors and vaccines based on non-segmented negatives stranded rna viruses |
| WO2000053766A1 (en) * | 1999-03-08 | 2000-09-14 | Stichting Dienst Landbouwkundig Onderzoek | Non-spreading pestivirus |
| WO2003029416A2 (en) * | 2001-10-01 | 2003-04-10 | Uab Research Foundation | Recombinant respiratory syncytial viruses with deleted surface glycoprotein genes and uses thereof |
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| PT702085E (pt) | 1994-07-18 | 2004-04-30 | Karl Klaus Conzelmann | Virus de arn de cadeia negativa nao segmentada infeccioso recombinante |
| AU727923B2 (en) | 1995-09-27 | 2001-01-04 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Production of infectious respiratory syncytial virus from cloned nucleotide sequences |
| EP0912724B1 (en) | 1996-07-15 | 2009-12-09 | The Government of The United States of America, as represented by The Department of Health and Human Services | Production of attenuated respiratory syncytial virus vaccines from cloned nucleotide sequences |
| AU2001263896A1 (en) | 2000-06-23 | 2002-01-14 | Akzo Nobel N.V. | Attenuated bovine respiratory syncytial virus |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996010400A1 (en) * | 1994-09-30 | 1996-04-11 | The Uab Research Foundation | Gene therapy vectors and vaccines based on non-segmented negatives stranded rna viruses |
| WO2000053766A1 (en) * | 1999-03-08 | 2000-09-14 | Stichting Dienst Landbouwkundig Onderzoek | Non-spreading pestivirus |
| WO2003029416A2 (en) * | 2001-10-01 | 2003-04-10 | Uab Research Foundation | Recombinant respiratory syncytial viruses with deleted surface glycoprotein genes and uses thereof |
Non-Patent Citations (4)
| Title |
|---|
| COLLINS P.L. ET AL: "PRODUCTION OF INFECTIOUS HUMAN RESPIRATORY SYNCYTIAL VIRUS FROM CLONED CDNA CONFIRMS AN ESSENTIAL ROLE FOR THE TRANSCRIPTION ELONGATION FACTOR FROM THE 5' PROXIMAL OPEN READING FRAME OF THE M2 MRNA IN GENE EXPRESSION AND PROVIDES A CAPABILITY FOR VACCINE DEVELOPMENT", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, US, vol. 92, 1 December 1995 (1995-12-01), pages 11563-11567, XP002066592, ISSN: 0027-8424, the whole document * |
| KARRON R.A. ET AL.: "Respiratory syncytial virus (RSV) SH and G proteins are not essential for viral replication in vitro: Clinical evaluation and molecular characterization of a cold-passaged, attenuated RSV subgroup B mutant", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, US, vol. 94, no. 25, 9 December 1997 (1997-12-09), pages 13961-13966, XP002154716, ISSN: 0027-8424, the whole document * |
| TECHAARPORNKUL S. ET AL.: "Functional analysis of recombinant respiratory syncytial virus deletion mutants lacking the small hydrophobic and/or attachment glycoprotein gene", JOURNAL OF VIROLOGY 2001 UNITED STATES, vol. 75, no. 15, 2001, pages 6825-6834, XP002301785, ISSN: 0022-538X, the whole document * |
| TENG MICHAEL N. ET AL.: "Contribution of the respiratory syncytial virus G glycoprotein and its secreted and membrane-bound forms to virus replication in vitro and in vivo", VIROLOGY, vol. 289, no. 2, 25 October 2001 (2001-10-25), pages 283-296, XP002301784, ISSN: 0042-6822, the whole document * |
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