EA010374B1 - Полипептиды с повышенной экспрессией - Google Patents

Полипептиды с повышенной экспрессией Download PDF

Info

Publication number
EA010374B1
EA010374B1 EA200700292A EA200700292A EA010374B1 EA 010374 B1 EA010374 B1 EA 010374B1 EA 200700292 A EA200700292 A EA 200700292A EA 200700292 A EA200700292 A EA 200700292A EA 010374 B1 EA010374 B1 EA 010374B1
Authority
EA
Eurasian Patent Office
Prior art keywords
polypeptide
composite
composite polypeptide
polypeptide according
antigen
Prior art date
Application number
EA200700292A
Other languages
English (en)
Russian (ru)
Other versions
EA200700292A1 (ru
Inventor
Патрик ХОФФМАНН
Силке Миттельштрасс
Енс Хеннеке
Тобиас РАУМ
Original Assignee
Микромет Аг
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Микромет Аг filed Critical Микромет Аг
Publication of EA200700292A1 publication Critical patent/EA200700292A1/xx
Publication of EA010374B1 publication Critical patent/EA010374B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Public Health (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
EA200700292A 2004-07-16 2005-07-15 Полипептиды с повышенной экспрессией EA010374B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP04016890 2004-07-16
PCT/EP2005/007748 WO2006008096A1 (en) 2004-07-16 2005-07-15 Expression-enhanced polypeptides

Publications (2)

Publication Number Publication Date
EA200700292A1 EA200700292A1 (ru) 2007-06-29
EA010374B1 true EA010374B1 (ru) 2008-08-29

Family

ID=35229693

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200700292A EA010374B1 (ru) 2004-07-16 2005-07-15 Полипептиды с повышенной экспрессией

Country Status (12)

Country Link
US (1) US8518403B2 (https=)
EP (1) EP1769000B1 (https=)
JP (2) JP5014988B2 (https=)
KR (1) KR20070042967A (https=)
CN (1) CN101018809B (https=)
AU (1) AU2005263555B2 (https=)
CA (1) CA2570990C (https=)
EA (1) EA010374B1 (https=)
MX (1) MX2007000387A (https=)
NZ (1) NZ552745A (https=)
WO (1) WO2006008096A1 (https=)
ZA (1) ZA200610478B (https=)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136311A (en) 1996-05-06 2000-10-24 Cornell Research Foundation, Inc. Treatment and diagnosis of cancer
WO2007109321A2 (en) 2006-03-20 2007-09-27 The Regents Of The University Of California Engineered anti-prostate stem cell antigen (psca) antibodies for cancer targeting
CN101245110B (zh) * 2007-02-16 2010-09-15 鲁南制药集团股份有限公司 重组中性粒细胞抑制因子和水蛭原嵌合蛋白及其药物组合物
US8940298B2 (en) * 2007-09-04 2015-01-27 The Regents Of The University Of California High affinity anti-prostate stem cell antigen (PSCA) antibodies for cancer targeting and detection
WO2009046294A2 (en) * 2007-10-03 2009-04-09 Cornell University Treatment of proliferative disorders using antibodies to psma
WO2010006454A2 (en) * 2008-06-30 2010-01-21 Esbatech, An Alcon Biomedical Research Unit Llc Functionalized polypeptides
US20100069616A1 (en) * 2008-08-06 2010-03-18 The Regents Of The University Of California Engineered antibody-nanoparticle conjugates
JP6126782B2 (ja) * 2008-10-01 2017-05-10 アムゲン リサーチ （ミュンヘン） ゲーエムベーハー 異種間特異的ｐｓｍａ×ｃｄ３二重特異性単鎖抗体
NZ591087A (en) * 2008-10-01 2012-08-31 Micromet Ag Cross-species-specific pscaxcd3, cd19xcd3, c-metxcd3, endosialinxcd3, epcamxc d3, igf-1rxcd3 or fapalpha xcd3 bispecific single chain antibody
EP2398504B1 (en) * 2009-02-17 2018-11-28 Cornell Research Foundation, Inc. Methods and kits for diagnosis of cancer and prediction of therapeutic value
JP2011026294A (ja) * 2009-06-26 2011-02-10 Canon Inc 化合物
JP2012531212A (ja) * 2009-07-03 2012-12-10 アビペップ ピーティーワイ リミテッド イムノコンジュゲート及びその作製方法
US8993715B2 (en) 2009-07-06 2015-03-31 Canon Kabushiki Kaisha Labeled protein and method for obtaining the same
CA2782333C (en) 2009-12-02 2019-06-04 Imaginab, Inc. J591 minibodies and cys-diabodies for targeting human prostate specific membrane antigen (psma) and methods for their use
SG181814A1 (en) 2009-12-23 2012-07-30 Avipep Pty Ltd Immuno-conjugates and methods for producing them 2
UA118950C2 (uk) 2011-04-22 2019-04-10 Аптево Рісьорч Енд Девелопмент Ллс Поліпептид, який зв'язує специфічний мембранний антиген простати та комплекс т-клітинного рецептора
MX361875B (es) * 2012-03-14 2018-12-18 Regeneron Pharma Moléculas de unión de antígeno multiespecifícas y usos de las mismas.
JO3519B1 (ar) 2013-01-25 2020-07-05 Amgen Inc تركيبات أجسام مضادة لأجل cdh19 و cd3
US9212225B1 (en) 2014-07-01 2015-12-15 Amphivena Therapeutics, Inc. Bispecific CD33 and CD3 binding proteins
MX2017015380A (es) 2015-05-29 2018-03-28 Amphivena Therapeutics Inc Metodos para utilizar proteinas de enlace biespecificas cd3 y cd33.
WO2017027325A1 (en) 2015-08-07 2017-02-16 Imaginab, Inc. Antigen binding constructs to target molecules
EP3352760B1 (en) 2015-09-21 2026-03-11 Aptevo Research and Development LLC Cd3 binding polypeptides
WO2017190079A1 (en) 2016-04-28 2017-11-02 Regeneron Pharmaceuticals, Inc. Methods of making multispecific antigen-binding molecules
WO2018147960A1 (en) 2017-02-08 2018-08-16 Imaginab, Inc. Extension sequences for diabodies
SG11201907299XA (en) 2017-02-08 2019-09-27 Dragonfly Therapeutics Inc Multi-specific binding proteins for activation of natural killer cells and therapeutic uses thereof to treat cancer
IL268755B2 (en) 2017-02-20 2025-12-01 Dragonfly Therapeutics Inc Proteins that bind her2, nkg2d, and cd16
DK3749346T3 (da) 2018-02-08 2024-09-09 Dragonfly Therapeutics Inc Antistof variable domænekombinationer rettet mod nkg2d-receptoren
EA202091887A1 (ru) 2018-02-08 2020-10-23 Драгонфлай Терапьютикс, Инк. Комбинированная терапия рака с применением мультиспецифических связывающих белков, которые активируют естественные клетки-киллеры
WO2019164930A1 (en) 2018-02-20 2019-08-29 Dragonfly Therapeutics, Inc. Multi-specific binding proteins that bind cd33, nkg2d, and cd16, and methods of use
US12378318B2 (en) 2018-08-08 2025-08-05 Dragonfly Therapeutics, Inc. Proteins binding NKG2D, CD16 and a tumor-associated antigen
EA202091888A1 (ru) 2018-08-08 2020-10-23 Драгонфлай Терапьютикс, Инк. Вариабельные домены антител, нацеленные на рецептор nkg2d
WO2020033630A1 (en) 2018-08-08 2020-02-13 Dragonfly Therapeutics, Inc. Multi-specific binding proteins that bind bcma, nkg2d and cd16, and methods of use
CN110668984B (zh) * 2019-12-03 2020-04-10 凯莱英医药集团（天津）股份有限公司 一种依特卡肽中间体及依特卡肽的合成方法
MX2022013944A (es) 2020-05-06 2022-11-30 Dragonfly Therapeutics Inc Proteinas que se unen al receptor activador de celulas asesinas naturales grupo 2 miembro d (nkg2d), cumulo de diferenciacion (cd16) y miembro a de la familia de dominios de lectina tipo c 12 (clec12a).
EP4247850A1 (en) 2020-11-20 2023-09-27 Simcere Innovation, Inc. Armed dual car-t compositions and methods for cancer immunotherapy
JP2024508894A (ja) 2021-03-03 2024-02-28 ドラゴンフライ セラピューティクス， インコーポレイテッド Ｎｋｇ２ｄ、ｃｄ１６、及び腫瘍関連抗原に結合する多特異性結合タンパク質を使用して癌を治療する方法
CN117285647A (zh) * 2022-06-17 2023-12-26 南京北恒生物科技有限公司 靶向ccr8的嵌合抗原受体及其用途

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5739277A (en) * 1995-04-14 1998-04-14 Genentech Inc. Altered polypeptides with increased half-life
WO2001004135A2 (en) * 1999-07-13 2001-01-18 The Regents Of The University Of Michigan Crosslinked dna condensate compositions and gene delivery methods
US20030044423A1 (en) * 2001-03-07 2003-03-06 Lexigen Pharmaceuticals Corp. Expression technology for proteins containing a hybrid isotype antibody moiety
US20040018586A1 (en) * 2000-05-16 2004-01-29 Rosendahl Mary S. Method for refolding proteins containing free cysteine residues

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5013653A (en) * 1987-03-20 1991-05-07 Creative Biomolecules, Inc. Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
US5932448A (en) * 1991-11-29 1999-08-03 Protein Design Labs., Inc. Bispecific antibody heterodimers
DE60105647T2 (de) * 2000-06-09 2005-09-22 The Government Of The United States Of America As Represented By The Department Of Health And Human Services Pegylation von linker verbessert die antitumor-aktivität und verringert die toxizität von immunkonjugaten
AU6845201A (en) * 2000-06-15 2001-12-24 Us Gov Health & Human Serv A recombinant non-replicating virus expressing GM-CSF and uses thereof to enhance immune responses
CN1195779C (zh) * 2001-05-24 2005-04-06 中国科学院遗传与发育生物学研究所 抗人卵巢癌抗人cd3双特异性抗体
HRP20040242A2 (en) * 2001-11-08 2005-02-28 Yongna Xing William Protein knobs
BRPI0819765A2 (pt) * 2007-11-30 2015-05-05 Bristol Myers Squibb Co Conjugados de anticorpos anti-rg-1

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5739277A (en) * 1995-04-14 1998-04-14 Genentech Inc. Altered polypeptides with increased half-life
WO2001004135A2 (en) * 1999-07-13 2001-01-18 The Regents Of The University Of Michigan Crosslinked dna condensate compositions and gene delivery methods
US20040018586A1 (en) * 2000-05-16 2004-01-29 Rosendahl Mary S. Method for refolding proteins containing free cysteine residues
US20030044423A1 (en) * 2001-03-07 2003-03-06 Lexigen Pharmaceuticals Corp. Expression technology for proteins containing a hybrid isotype antibody moiety

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LUO D. ET AL.: "AN ENGINEERED BIVALENT SINGLE-CHAIN ANTIBODY FRAGMENT THAT INCREASES ANTIGEN BINDING ACTIVITY". JOURNAL OF BIOCHEMISTRY, JAPANESE, BIOCHEMICAL SOCIETY, TOKYO, JP, vol. 121, no. 5, 1997, pages 831-834, XP009051057, ISSN: 0021-924X, cited in the application, abstract *
STEVENSON G.T. ET AL.: "Conjugation of human Fcgamma in closed-hinge or open-hinge configuration to Fab' gamma and analogous ligands". JOURNAL OF IMMUNOLOGY, THE WILLIAMS AND WILKINS CO. BALTIMORE, US, vol. 158, 1997, pages 2242-2250, XP002955118, ISSN: 0022-1767, the whole document *

Also Published As

Publication number Publication date
US8518403B2 (en) 2013-08-27
US20090053223A1 (en) 2009-02-26
NZ552745A (en) 2009-01-31
JP2008506659A (ja) 2008-03-06
CN101018809B (zh) 2012-07-18
AU2005263555B2 (en) 2011-01-27
MX2007000387A (es) 2007-03-28
EP1769000A1 (en) 2007-04-04
AU2005263555A1 (en) 2006-01-26
EA200700292A1 (ru) 2007-06-29
ZA200610478B (en) 2008-04-30
CN101018809A (zh) 2007-08-15
WO2006008096A1 (en) 2006-01-26
JP5014988B2 (ja) 2012-08-29
CA2570990A1 (en) 2006-01-26
JP2012144552A (ja) 2012-08-02
EP1769000B1 (en) 2014-12-24
KR20070042967A (ko) 2007-04-24
CA2570990C (en) 2014-01-21

Similar Documents

Publication Publication Date Title
EA010374B1 (ru) Полипептиды с повышенной экспрессией
CA1341411C (en) Method for producing fv fragments in eukaryotic cells
AU675223B2 (en) Chimeric multivalent protein analogues and methods of use thereof
JP3280376B2 (ja) 多価抗原結合性蛋白質
JP3490437B2 (ja) 単量体および二量体抗体フラグメント融合タンパク質
CA2116774C (en) Expression in e. coli antibody fragments having at least a cysteine present as a free thiol. use for the production of bifunctional f(ab') 2 antibodies
EP0894135B1 (en) Multivalent and multispecific antigen-binding protein
JP2008506659A5 (https=)
US20100105876A1 (en) Method for the oligomerisation of peptides
CA2315944A1 (en) Bifunctional molecules
US5965405A (en) Method for producing Fv fragments in eukaryotic cells
Anand et al. Synthesis and expression in Escherichia coli of cistronic DNA encoding an antibody fragment specific for a Salmonella serotype B O-antigen
US20030148406A1 (en) Multivalent antigen-binding proteins
CN119998326A (zh) 具有新型二硫键的超稳定抗体片段
Kim et al. Properties of GST-CALM expressed in E. coli
Sepp et al. Inhibition of expression of the Galα1-3Gal epitope on porcine cells using an intracellular single-chain antibody directed against α1, 3Galactosyltransferase
KR100832773B1 (ko) 기능성 Ｆｖ 항체 절편 제조 방법
AU748007B2 (en) Bifunctional molecules
Chiswell et al. Binding of the lipocalin C8γ to human complement protein C8α is mediated by loops located at the entrance to the C8γ ligand binding site
Chang et al. Structural factors affect the interactions of anticardiotoxin antibodies and cobra venom cardiotoxins
Wibbenmeyer Testing the structural basis of the HyHEL-5/lysozyme interaction

Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM RU