DK3471749T3 - Fremgangsmåder og sammensætninger til detektering af en mål-RNA - Google Patents
Fremgangsmåder og sammensætninger til detektering af en mål-RNA Download PDFInfo
- Publication number
- DK3471749T3 DK3471749T3 DK17813959.8T DK17813959T DK3471749T3 DK 3471749 T3 DK3471749 T3 DK 3471749T3 DK 17813959 T DK17813959 T DK 17813959T DK 3471749 T3 DK3471749 T3 DK 3471749T3
- Authority
- DK
- Denmark
- Prior art keywords
- compositions
- detecting
- methods
- target rna
- rna
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title 1
- QVTZSEYZNINDJJ-UHFFFAOYSA-N CCC(CC1)=CC1I Chemical compound CCC(CC1)=CC1I QVTZSEYZNINDJJ-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/307—Single strand endonuclease
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/101—Interaction between at least two labels
- C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662351172P | 2016-06-16 | 2016-06-16 | |
US201662378156P | 2016-08-22 | 2016-08-22 | |
US15/467,922 US10337051B2 (en) | 2016-06-16 | 2017-03-23 | Methods and compositions for detecting a target RNA |
PCT/US2017/037308 WO2017218573A1 (en) | 2016-06-16 | 2017-06-13 | Methods and compositions for detecting a target rna |
Publications (1)
Publication Number | Publication Date |
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DK3471749T3 true DK3471749T3 (da) | 2022-02-07 |
Family
ID=60661224
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK17813959.8T DK3471749T3 (da) | 2016-06-16 | 2017-06-13 | Fremgangsmåder og sammensætninger til detektering af en mål-RNA |
Country Status (11)
Country | Link |
---|---|
US (6) | US10337051B2 (da) |
EP (2) | EP4036249A1 (da) |
JP (2) | JP2019522472A (da) |
CN (2) | CN116287127A (da) |
AU (2) | AU2017283538B2 (da) |
CA (1) | CA3024883A1 (da) |
DE (3) | DE212017000062U1 (da) |
DK (1) | DK3471749T3 (da) |
ES (1) | ES2908484T3 (da) |
GB (1) | GB2557153B (da) |
WO (1) | WO2017218573A1 (da) |
Families Citing this family (58)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA3012631A1 (en) * | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Novel crispr enzymes and systems |
US10337051B2 (en) * | 2016-06-16 | 2019-07-02 | The Regents Of The University Of California | Methods and compositions for detecting a target RNA |
JP7267013B2 (ja) * | 2016-06-17 | 2023-05-01 | ザ・ブロード・インスティテュート・インコーポレイテッド | Vi型crisprオルソログ及び系 |
WO2018064352A1 (en) | 2016-09-30 | 2018-04-05 | The Regents Of The University Of California | Rna-guided nucleic acid modifying enzymes and methods of use thereof |
WO2018064371A1 (en) | 2016-09-30 | 2018-04-05 | The Regents Of The University Of California | Rna-guided nucleic acid modifying enzymes and methods of use thereof |
EP4119663A1 (en) * | 2016-12-09 | 2023-01-18 | The Broad Institute, Inc. | Crispr effector system based diagnostics |
US11174515B2 (en) * | 2017-03-15 | 2021-11-16 | The Broad Institute, Inc. | CRISPR effector system based diagnostics |
BR112019019087A2 (pt) * | 2017-03-15 | 2020-05-12 | The Broad Institute, Inc. | Diagnóstico baseado em sistema efetor de crispr para detecção de vírus |
US11021740B2 (en) * | 2017-03-15 | 2021-06-01 | The Broad Institute, Inc. | Devices for CRISPR effector system based diagnostics |
US11104937B2 (en) * | 2017-03-15 | 2021-08-31 | The Broad Institute, Inc. | CRISPR effector system based diagnostics |
WO2018195545A2 (en) | 2017-04-21 | 2018-10-25 | The General Hospital Corporation | Variants of cpf1 (cas12a) with altered pam specificity |
US10392616B2 (en) | 2017-06-30 | 2019-08-27 | Arbor Biotechnologies, Inc. | CRISPR RNA targeting enzymes and systems and uses thereof |
US20200165594A1 (en) * | 2017-07-07 | 2020-05-28 | The Broad Institute, Inc. | Crispr system based antiviral therapy |
US20190218532A1 (en) * | 2017-09-19 | 2019-07-18 | Massachusetts Institute Of Technology | Streptococcus Canis Cas9 as a Genome Engineering Platform with Novel PAM Specificity |
US11633732B2 (en) | 2017-10-04 | 2023-04-25 | The Broad Institute, Inc. | CRISPR effector system based diagnostics |
US11970719B2 (en) | 2017-11-01 | 2024-04-30 | The Regents Of The University Of California | Class 2 CRISPR/Cas compositions and methods of use |
CA3080493A1 (en) | 2017-11-01 | 2019-05-09 | The Regents Of The University Of California | Casz compositions and methods of use |
US11661599B1 (en) | 2017-12-14 | 2023-05-30 | National Technology & Engineering Solutions Of Sandia, Llc | CRISPR-Cas based system for targeting single-stranded sequences |
EP3765616B1 (en) | 2018-03-14 | 2023-07-19 | Arbor Biotechnologies, Inc. | Novel crispr dna and rna targeting enzymes and systems |
EP4257696A3 (en) | 2018-03-14 | 2024-01-24 | Arbor Biotechnologies, Inc. | Novel crispr dna targeting enzymes and systems |
WO2019204210A1 (en) * | 2018-04-16 | 2019-10-24 | Georgia Tech Research Corporation | Mrna driven expression of rna editors for treatment of pathologies |
CN110551800B (zh) * | 2018-06-03 | 2022-08-19 | 上海吐露港生物科技有限公司 | 一种耐高温Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒 |
US11608519B2 (en) | 2018-07-30 | 2023-03-21 | Tokitae Llc | Specific detection of deoxyribonucleic acid sequences using novel CRISPR enzyme-mediated detection strategies |
SG11202101934SA (en) * | 2018-07-30 | 2021-03-30 | Readcoor Llc | Methods and systems for sample processing or analysis |
EP3830301A1 (en) | 2018-08-01 | 2021-06-09 | Mammoth Biosciences, Inc. | Programmable nuclease compositions and methods of use thereof |
CN109055499B (zh) * | 2018-08-30 | 2021-01-19 | 杭州杰毅生物技术有限公司 | 基于CRISPR-Cas的等温核酸检测方法及试剂盒 |
WO2020051452A2 (en) * | 2018-09-07 | 2020-03-12 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Hiv or hcv detection with crispr-cas13a |
US20210396756A1 (en) * | 2018-10-03 | 2021-12-23 | The Broad Institute, Inc. | Crispr effector system based diagnostics for hemorrhagic fever detection |
WO2020142754A2 (en) | 2019-01-04 | 2020-07-09 | Mammoth Biosciences, Inc. | Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection |
CN113811607A (zh) | 2019-03-07 | 2021-12-17 | 加利福尼亚大学董事会 | CRISPR-Cas效应子多肽和其使用方法 |
KR20210142690A (ko) | 2019-03-21 | 2021-11-25 | 셜록 바이오사이언스, 인크. | 시스템 |
JPWO2021002476A1 (da) * | 2019-07-04 | 2021-01-07 | ||
US20220364159A1 (en) * | 2019-07-26 | 2022-11-17 | Mammoth Biosciences, Inc. | Compositions for detection of dna and methods of use thereof |
WO2021062201A1 (en) * | 2019-09-25 | 2021-04-01 | Spotlight Therapeutics | Compositions and methods for nucleoprotein targeting and expression |
CN110732028B (zh) * | 2019-11-01 | 2022-07-26 | 南开大学 | 一种可限制激活CRISPR/Cas13a的双锁纳米粒子的制备方法和应用 |
US20230139524A1 (en) * | 2020-02-27 | 2023-05-04 | Lsk Technologies Inc. | Systems and methods for characterization of an assay from regions of interest using optical reactions |
EP4121568A2 (en) * | 2020-03-19 | 2023-01-25 | The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone | Rapid field-deployable detection of sars-cov-2 virus |
CN113549605B (zh) * | 2020-04-26 | 2023-03-31 | 生物岛实验室 | 用于抑制SARS-CoV-2的组合物及载体系统 |
CN116157536A (zh) * | 2020-05-19 | 2023-05-23 | 加利福尼亚大学董事会 | 用于核酸检测的核酸酶链反应的组合物和方法 |
WO2021243276A1 (en) * | 2020-05-29 | 2021-12-02 | University Of Florida Research Foundation | Crispr/cas chain reaction systems and methods for amplifying the detection sensitivity of crispr-based target detection |
CA3186580A1 (en) | 2020-06-12 | 2021-12-16 | Sherlock Biosciences, Inc. | Crispr-based sars-cov-2 detection |
CN116024314A (zh) * | 2020-09-18 | 2023-04-28 | 山东舜丰生物科技有限公司 | 基于crispr技术进行靶核酸多重检测的方法 |
WO2022068912A1 (en) | 2020-09-30 | 2022-04-07 | Huigene Therapeutics Co., Ltd. | Engineered crispr/cas13 system and uses thereof |
WO2022188039A1 (en) | 2021-03-09 | 2022-09-15 | Huigene Therapeutics Co., Ltd. | Engineered crispr/cas13 system and uses thereof |
CN112143821A (zh) * | 2020-09-30 | 2020-12-29 | 北京师范大学 | Fret检测白色念珠菌,新生隐球菌和肺炎克雷伯杆菌的试剂及其应用 |
CN112813179B (zh) * | 2020-12-09 | 2023-04-18 | 广州市第一人民医院(广州消化疾病中心、广州医科大学附属市一人民医院、华南理工大学附属第二医院) | 一种用于尿路感染病原菌检测的试剂盒及其使用方法 |
WO2022122689A1 (en) * | 2020-12-09 | 2022-06-16 | BioNTech SE | Rna manufacturing |
EP4291678A1 (en) | 2021-02-09 | 2023-12-20 | Sherlock Biosciences, Inc. | Nucleic acid amplification using promoter primers |
WO2022241032A1 (en) * | 2021-05-12 | 2022-11-17 | Mammoth Biosciences, Inc. | Enhanced guide nucleic acids and methods of use |
US20220389418A1 (en) | 2021-06-01 | 2022-12-08 | Helmholtz-Institut Für Rna-Basierte Nfektionsforschung (Hzi) | Rna-guided cas nucleases and uses thereof in diagnostics and therapy |
WO2023287669A2 (en) * | 2021-07-12 | 2023-01-19 | Labsimply, Inc. | Nuclease cascade assay |
WO2023004391A2 (en) | 2021-07-21 | 2023-01-26 | Montana State University | Nucleic acid detection using type iii crispr complex |
WO2023114090A2 (en) | 2021-12-13 | 2023-06-22 | Labsimply, Inc. | Signal boost cascade assay |
US11820983B2 (en) | 2021-12-13 | 2023-11-21 | Labsimply, Inc. | Tuning cascade assay kinetics via molecular design |
WO2023112886A1 (ja) * | 2021-12-16 | 2023-06-22 | 国立研究開発法人理化学研究所 | 1本鎖rnaの製造方法 |
WO2024020352A1 (en) * | 2022-07-18 | 2024-01-25 | Vertex Pharmaceuticals Incorporated | Tandem guide rnas (tg-rnas) and their use in genome editing |
WO2024059197A1 (en) * | 2022-09-14 | 2024-03-21 | Revvity Health Sciences, Inc. | Methods and compositions for single-stranded nucleic acid ladders by guided cleavage |
CN116814627A (zh) * | 2023-07-11 | 2023-09-29 | 深圳市辰景生命科技有限公司 | sgRNA组合物、检测新型冠状病毒SARS-CoV-2的试剂盒以及检测方法 |
Family Cites Families (76)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3687808A (en) | 1969-08-14 | 1972-08-29 | Univ Leland Stanford Junior | Synthetic polynucleotides |
US5034506A (en) | 1985-03-15 | 1991-07-23 | Anti-Gene Development Group | Uncharged morpholino-based polymers having achiral intersubunit linkages |
US5602240A (en) | 1990-07-27 | 1997-02-11 | Ciba Geigy Ag. | Backbone modified oligonucleotide analogs |
US5489677A (en) | 1990-07-27 | 1996-02-06 | Isis Pharmaceuticals, Inc. | Oligonucleoside linkages containing adjacent oxygen and nitrogen atoms |
US5714331A (en) | 1991-05-24 | 1998-02-03 | Buchardt, Deceased; Ole | Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility |
US5539082A (en) | 1993-04-26 | 1996-07-23 | Nielsen; Peter E. | Peptide nucleic acids |
US5719262A (en) | 1993-11-22 | 1998-02-17 | Buchardt, Deceased; Ole | Peptide nucleic acids having amino acid side chains |
JP3756313B2 (ja) | 1997-03-07 | 2006-03-15 | 武 今西 | 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体 |
CN1273476C (zh) | 1997-09-12 | 2006-09-06 | 埃克西康有限公司 | 寡核苷酸类似物 |
ATE270344T1 (de) | 1999-11-16 | 2004-07-15 | Atto Tec Gmbh | Farbstoffmarkiertes oligonukleotid zum markieren eines nukleinsäuremoleküls |
US6727356B1 (en) | 1999-12-08 | 2004-04-27 | Epoch Pharmaceuticals, Inc. | Fluorescent quenching detection reagents and methods |
US7019129B1 (en) | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
JP2004521606A (ja) * | 2000-05-24 | 2004-07-22 | サード・ウェーブ・テクノロジーズ・インク | Rnaの検出法 |
US6773885B1 (en) | 2000-09-29 | 2004-08-10 | Integrated Dna Technologies, Inc. | Compositions and methods for visual ribonuclease detection assays |
US6818420B2 (en) | 2002-02-27 | 2004-11-16 | Biosource International, Inc. | Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same |
CN1886512A (zh) | 2002-04-23 | 2006-12-27 | 斯克里普斯研究所 | 多肽在叶绿体中的表达以及用于表达多肽的组合物和方法 |
DE10259374A1 (de) | 2002-12-18 | 2004-07-08 | Atto-Tec Gmbh | Carboxamid-substituierte Farbstoffe für analytische Anwendungen |
DE10329860A1 (de) | 2003-07-02 | 2005-01-20 | Atto-Tec Gmbh | Sulfonamidderivate polycyclischer Farbstoffe für analytische Anwendungen |
EP1580273A1 (en) | 2004-03-26 | 2005-09-28 | Friedrich-Alexander-Universität Erlangen-Nürnberg | Peptide-based method for monitoring gene expression in a host cell |
US8586718B2 (en) | 2004-09-14 | 2013-11-19 | Applied Biosystems, Llc | Multi-chromophoric quencher constructs for use in high sensitivity energy transfer probes |
CN101283089A (zh) | 2005-10-07 | 2008-10-08 | 加利福尼亚大学董事会 | 修饰细胞色素p450酶的编码核酸和其应用方法 |
US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
EP2268608A4 (en) | 2008-04-01 | 2012-01-11 | Biosearch Technologies Inc | DARK NUCLEIC ACID STABILIZED FLUOROPHORE DEACTIVATOR PROBES |
WO2011053871A2 (en) | 2009-10-30 | 2011-05-05 | Life Technologies Corporation | Multi-primer assay for mycoplasma detection |
WO2011100749A2 (en) | 2010-02-15 | 2011-08-18 | Cascade Biosystems, Inc. | Methods and materials for detecting viral or microbial infections |
EP2580354A4 (en) | 2010-06-11 | 2013-10-30 | Pathogenica Inc | NUCLEIC ACIDS FOR MULTIPLEX DETECTION OF ORGANISMS AND METHOD FOR THEIR USE AND MANUFACTURE |
DE102010042634A1 (de) | 2010-10-19 | 2012-04-19 | Atto-Tec Gmbh | Neue Amin-substituierte tricyclische Fluoreszenzfarbstoffe |
US9730967B2 (en) | 2011-02-04 | 2017-08-15 | Katherine Rose Kovarik | Method and system for treating cancer cachexia |
ES2755934T3 (es) | 2011-10-31 | 2020-04-24 | Eiken Chemical | Método para detectar ácido nucleico diana |
US8815782B2 (en) | 2011-11-11 | 2014-08-26 | Agilent Technologies, Inc. | Use of DNAzymes for analysis of an RNA sample |
LT3401400T (lt) | 2012-05-25 | 2019-06-10 | The Regents Of The University Of California | Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui |
US20140024024A1 (en) * | 2012-07-17 | 2014-01-23 | General Electric Company | Methods of detecting dna, rna and protein in biological samples |
EP3138911B1 (en) | 2012-12-06 | 2018-12-05 | Sigma Aldrich Co. LLC | Crispr-based genome modification and regulation |
CN103088128B (zh) * | 2013-01-09 | 2015-11-18 | 华中科技大学 | 一种microRNA的二次循环扩增检测方法及试剂盒 |
KR101780885B1 (ko) | 2013-03-14 | 2017-10-11 | 카리부 바이오사이언시스 인코포레이티드 | 핵산-표적화 핵산의 조성물 및 방법 |
US9234213B2 (en) * | 2013-03-15 | 2016-01-12 | System Biosciences, Llc | Compositions and methods directed to CRISPR/Cas genomic engineering systems |
EP3071695A2 (en) | 2013-11-18 | 2016-09-28 | Crispr Therapeutics AG | Crispr-cas system materials and methods |
US9994831B2 (en) | 2013-12-12 | 2018-06-12 | The Regents Of The University Of California | Methods and compositions for modifying a single stranded target nucleic acid |
US9850525B2 (en) | 2014-01-29 | 2017-12-26 | Agilent Technologies, Inc. | CAS9-based isothermal method of detection of specific DNA sequence |
EP3126498A4 (en) | 2014-03-20 | 2017-08-23 | Université Laval | Crispr-based methods and products for increasing frataxin levels and uses thereof |
EP3129488B1 (en) | 2014-04-10 | 2019-06-12 | The Regents of The University of California | Methods and compositions for using argonaute to modify a single stranded target nucleic acid |
CA2951707A1 (en) | 2014-06-10 | 2015-12-17 | Massachusetts Institute Of Technology | Method for gene editing |
KR20170036801A (ko) | 2014-08-19 | 2017-04-03 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산의 프로빙 및 맵핑을 위한 rna-가이드된 시스템 |
WO2016094872A1 (en) | 2014-12-12 | 2016-06-16 | The Broad Institute Inc. | Dead guides for crispr transcription factors |
EP3985115A1 (en) | 2014-12-12 | 2022-04-20 | The Broad Institute, Inc. | Protected guide rnas (pgrnas) |
WO2016106236A1 (en) | 2014-12-23 | 2016-06-30 | The Broad Institute Inc. | Rna-targeting system |
US11180792B2 (en) | 2015-01-28 | 2021-11-23 | The Regents Of The University Of California | Methods and compositions for labeling a single-stranded target nucleic acid |
US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
WO2016205749A1 (en) | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Novel crispr enzymes and systems |
AU2016280893B2 (en) | 2015-06-18 | 2021-12-02 | Massachusetts Institute Of Technology | CRISPR enzyme mutations reducing off-target effects |
CA3012631A1 (en) * | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Novel crispr enzymes and systems |
US9580727B1 (en) | 2015-08-07 | 2017-02-28 | Caribou Biosciences, Inc. | Compositions and methods of engineered CRISPR-Cas9 systems using split-nexus Cas9-associated polynucleotides |
US20170211142A1 (en) | 2015-10-22 | 2017-07-27 | The Broad Institute, Inc. | Novel crispr enzymes and systems |
EP3400299B1 (en) | 2016-01-08 | 2020-11-11 | University of Georgia Research Foundation, Inc. | Methods for cleaving dna and rna molecules |
US11441146B2 (en) | 2016-01-11 | 2022-09-13 | Christiana Care Health Services, Inc. | Compositions and methods for improving homogeneity of DNA generated using a CRISPR/Cas9 cleavage system |
US9896696B2 (en) | 2016-02-15 | 2018-02-20 | Benson Hill Biosystems, Inc. | Compositions and methods for modifying genomes |
US20190024075A1 (en) | 2016-02-23 | 2019-01-24 | Arc Bio, Llc | Methods and compositions for target detection |
US20190093091A1 (en) | 2016-04-06 | 2019-03-28 | Temple University - Of The Commonwealth System Of Higher Education | Compositions for eradicating flavivirus infections in subjects |
WO2017205668A1 (en) | 2016-05-25 | 2017-11-30 | Arizona Board Of Regents On Behalf Of Arizona State University | Portable, low-cost pathogen detection and strain identification platform |
US10337051B2 (en) | 2016-06-16 | 2019-07-02 | The Regents Of The University Of California | Methods and compositions for detecting a target RNA |
JP7267013B2 (ja) | 2016-06-17 | 2023-05-01 | ザ・ブロード・インスティテュート・インコーポレイテッド | Vi型crisprオルソログ及び系 |
AU2017280353B2 (en) | 2016-06-24 | 2021-11-11 | Inscripta, Inc. | Methods for generating barcoded combinatorial libraries |
US20210166783A1 (en) | 2016-08-17 | 2021-06-03 | The Broad Institute, Inc. | Methods for identifying class 2 crispr-cas systems |
WO2018064371A1 (en) | 2016-09-30 | 2018-04-05 | The Regents Of The University Of California | Rna-guided nucleic acid modifying enzymes and methods of use thereof |
WO2018064352A1 (en) | 2016-09-30 | 2018-04-05 | The Regents Of The University Of California | Rna-guided nucleic acid modifying enzymes and methods of use thereof |
CN106701830B (zh) | 2016-12-07 | 2020-01-03 | 湖南人文科技学院 | 一种敲除猪胚胎p66shc基因的方法 |
EP4119663A1 (en) | 2016-12-09 | 2023-01-18 | The Broad Institute, Inc. | Crispr effector system based diagnostics |
AU2018240515A1 (en) * | 2017-03-24 | 2019-08-01 | CureVac SE | Nucleic acids encoding CRISPR-associated proteins and uses thereof |
WO2018195545A2 (en) | 2017-04-21 | 2018-10-25 | The General Hospital Corporation | Variants of cpf1 (cas12a) with altered pam specificity |
ES2960390T3 (es) | 2017-08-09 | 2024-03-04 | Ricetec Inc | Composiciones y métodos para modificar genomas |
US20200255858A1 (en) | 2017-11-01 | 2020-08-13 | Jillian F. Banfield | Casy compositions and methods of use |
CA3080493A1 (en) | 2017-11-01 | 2019-05-09 | The Regents Of The University Of California | Casz compositions and methods of use |
GB2582100B (en) | 2017-11-01 | 2023-05-17 | Univ California | CAS12C Compositions and methods of use |
US11970719B2 (en) | 2017-11-01 | 2024-04-30 | The Regents Of The University Of California | Class 2 CRISPR/Cas compositions and methods of use |
US10253365B1 (en) | 2017-11-22 | 2019-04-09 | The Regents Of The University Of California | Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs |
CN111836903A (zh) | 2017-12-22 | 2020-10-27 | 博德研究所 | 基于crispr效应系统的多重诊断 |
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