BR112022018769A2 - Detecção rápida do vírus sars-cov-2 implementável no campo - Google Patents

Detecção rápida do vírus sars-cov-2 implementável no campo

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Publication number
BR112022018769A2
BR112022018769A2 BR112022018769A BR112022018769A BR112022018769A2 BR 112022018769 A2 BR112022018769 A2 BR 112022018769A2 BR 112022018769 A BR112022018769 A BR 112022018769A BR 112022018769 A BR112022018769 A BR 112022018769A BR 112022018769 A2 BR112022018769 A2 BR 112022018769A2
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BR
Brazil
Prior art keywords
cov
sars
implementable
virus
field
Prior art date
Application number
BR112022018769A
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English (en)
Inventor
Ott Melanie
Fozouni Parinaz
A Doudna Jennifer
A Fletcher Daniel
Savage David
Charles Emeric
Son Sungmin
Renuka Kumar Gagandeep
Switz Neil
Original Assignee
The J David Gladstone Inst A Testamentary Trust Established Under The Will Of J David Gladstone
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by The J David Gladstone Inst A Testamentary Trust Established Under The Will Of J David Gladstone, Univ California filed Critical The J David Gladstone Inst A Testamentary Trust Established Under The Will Of J David Gladstone
Publication of BR112022018769A2 publication Critical patent/BR112022018769A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6469—Cavity, e.g. ellipsoid

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

DETECÇÃO RÁPIDA DO VÍRUS SARS-COV-2 IMPLEMENTÁVEL NO CAMPO. A presente divulgação refere-se a métodos que usam a enzima CRISPR-Cas13, complexada com RNAs guia de crRNA de SARS-CoV-2 para detectar e quantificar a presença de RNA de SARS-CoV-2 em uma amostra com especificidade e sensibilidade aprimoradas. Esses métodos podem ser usados para diagnosticar a infecção por SARS-CoV-2, quantificar a concentração de RNA de SARS-CoV-2 presente em uma amostra, identificar a presença de diferentes variantes, subtipos ou mutações de emenda de SARS-CoV-2 e monitorar reativação da transcrição de SARS-CoV-2.
BR112022018769A 2020-03-19 2021-03-18 Detecção rápida do vírus sars-cov-2 implementável no campo BR112022018769A2 (pt)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US202062991827P 2020-03-19 2020-03-19
US202063057082P 2020-07-27 2020-07-27
US202062706488P 2020-08-19 2020-08-19
US202063081168P 2020-09-21 2020-09-21
US202163158297P 2021-03-08 2021-03-08
PCT/US2021/023025 WO2021188830A2 (en) 2020-03-19 2021-03-18 RAPID FIELD-DEPLOYABLE DETECTION OF SARS-CoV-2 VIRUS

Publications (1)

Publication Number Publication Date
BR112022018769A2 true BR112022018769A2 (pt) 2022-12-20

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BR112022018769A BR112022018769A2 (pt) 2020-03-19 2021-03-18 Detecção rápida do vírus sars-cov-2 implementável no campo

Country Status (12)

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US (1) US20210348243A1 (pt)
EP (1) EP4121568A2 (pt)
JP (1) JP2023520754A (pt)
KR (1) KR20230003470A (pt)
CN (1) CN116438303A (pt)
AU (1) AU2021236681A1 (pt)
BR (1) BR112022018769A2 (pt)
CA (1) CA3178847A1 (pt)
IL (1) IL296569A (pt)
MX (1) MX2022011641A (pt)
WO (1) WO2021188830A2 (pt)
ZA (1) ZA202211358B (pt)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113699148B (zh) * 2021-07-15 2024-01-09 四川大学 一种超灵敏抗体检测方法
US11814689B2 (en) 2021-07-21 2023-11-14 Montana State University Nucleic acid detection using type III CRISPR complex
CN113817873A (zh) * 2021-09-30 2021-12-21 华南理工大学 一种用于新冠病毒d614g突变的检测方法及试剂盒
US20230167485A1 (en) * 2021-11-29 2023-06-01 Microsoft Technology Licensing, Llc Multiplex assay for nucleic acid detection
CN113913406B (zh) * 2021-12-14 2022-04-08 中国人民解放军军事科学院军事医学研究院 一种用于检测SARS-CoV-2 69:70del位点的方法
CN114236124B (zh) * 2021-12-16 2023-06-09 军事科学院军事医学研究院环境医学与作业医学研究所 一种基于等温扩增和Cas14a双重放大的检测肌酸激酶同工酶的试剂盒
CN114350854B (zh) * 2022-01-10 2023-08-01 中国人民解放军军事科学院军事医学研究院 一种基于RAA-CRISPR检测SARS-CoV-2 69-70del位点的方法
CN114807316B (zh) * 2022-03-11 2023-02-03 北京科技大学 一种无核酸扩增可视化的rna定量检测方法
WO2023201275A1 (en) * 2022-04-13 2023-10-19 The Board Of Trustees Of The Leland Stanford Junior University Enzyme kinetics analyses of crispr endonucleases
WO2023235887A1 (en) * 2022-06-03 2023-12-07 Tpb Management Llc Computational multiplexing and application thereof
WO2024011208A1 (en) 2022-07-08 2024-01-11 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone VIRAL DIAGNOSTIC USING CRISPR RNA COMBINATIONS AND Cas13a ENZYME
WO2024091905A2 (en) * 2022-10-27 2024-05-02 University Of Rochester Compositions, methods and systems for high-fidelity cas13a variants with improved specificity

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6867851B2 (en) * 1999-11-04 2005-03-15 Regents Of The University Of Minnesota Scanning of biological samples
US7593157B2 (en) * 2004-11-29 2009-09-22 Nikon Corporation Zoom microscope
US7813013B2 (en) * 2006-11-21 2010-10-12 Illumina, Inc. Hexagonal site line scanning method and system
US20090162863A1 (en) * 2007-12-13 2009-06-25 Hitachi High-Technologies Corporation Nucleic acid detection probe
US10337051B2 (en) * 2016-06-16 2019-07-02 The Regents Of The University Of California Methods and compositions for detecting a target RNA
WO2020051452A2 (en) * 2018-09-07 2020-03-12 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Hiv or hcv detection with crispr-cas13a

Also Published As

Publication number Publication date
KR20230003470A (ko) 2023-01-06
MX2022011641A (es) 2022-12-08
IL296569A (en) 2022-11-01
CA3178847A1 (en) 2021-09-23
AU2021236681A1 (en) 2022-11-17
ZA202211358B (en) 2024-02-28
EP4121568A2 (en) 2023-01-25
CN116438303A (zh) 2023-07-14
WO2021188830A2 (en) 2021-09-23
JP2023520754A (ja) 2023-05-19
US20210348243A1 (en) 2021-11-11
WO2021188830A3 (en) 2021-11-11

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