BR112022018769A2 - Detecção rápida do vírus sars-cov-2 implementável no campo - Google Patents
Detecção rápida do vírus sars-cov-2 implementável no campoInfo
- Publication number
- BR112022018769A2 BR112022018769A2 BR112022018769A BR112022018769A BR112022018769A2 BR 112022018769 A2 BR112022018769 A2 BR 112022018769A2 BR 112022018769 A BR112022018769 A BR 112022018769A BR 112022018769 A BR112022018769 A BR 112022018769A BR 112022018769 A2 BR112022018769 A2 BR 112022018769A2
- Authority
- BR
- Brazil
- Prior art keywords
- cov
- sars
- implementable
- virus
- field
- Prior art date
Links
- 241000700605 Viruses Species 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 title abstract 2
- 241001678559 COVID-19 virus Species 0.000 abstract 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 208000025721 COVID-19 Diseases 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 108020005004 Guide RNA Proteins 0.000 abstract 1
- 208000037847 SARS-CoV-2-infection Diseases 0.000 abstract 1
- 201000003176 Severe Acute Respiratory Syndrome Diseases 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 230000007420 reactivation Effects 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6469—Cavity, e.g. ellipsoid
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
DETECÇÃO RÁPIDA DO VÍRUS SARS-COV-2 IMPLEMENTÁVEL NO CAMPO. A presente divulgação refere-se a métodos que usam a enzima CRISPR-Cas13, complexada com RNAs guia de crRNA de SARS-CoV-2 para detectar e quantificar a presença de RNA de SARS-CoV-2 em uma amostra com especificidade e sensibilidade aprimoradas. Esses métodos podem ser usados para diagnosticar a infecção por SARS-CoV-2, quantificar a concentração de RNA de SARS-CoV-2 presente em uma amostra, identificar a presença de diferentes variantes, subtipos ou mutações de emenda de SARS-CoV-2 e monitorar reativação da transcrição de SARS-CoV-2.
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202062991827P | 2020-03-19 | 2020-03-19 | |
US202063057082P | 2020-07-27 | 2020-07-27 | |
US202062706488P | 2020-08-19 | 2020-08-19 | |
US202063081168P | 2020-09-21 | 2020-09-21 | |
US202163158297P | 2021-03-08 | 2021-03-08 | |
PCT/US2021/023025 WO2021188830A2 (en) | 2020-03-19 | 2021-03-18 | RAPID FIELD-DEPLOYABLE DETECTION OF SARS-CoV-2 VIRUS |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112022018769A2 true BR112022018769A2 (pt) | 2022-12-20 |
Family
ID=77771406
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112022018769A BR112022018769A2 (pt) | 2020-03-19 | 2021-03-18 | Detecção rápida do vírus sars-cov-2 implementável no campo |
Country Status (12)
Country | Link |
---|---|
US (1) | US20210348243A1 (pt) |
EP (1) | EP4121568A2 (pt) |
JP (1) | JP2023520754A (pt) |
KR (1) | KR20230003470A (pt) |
CN (1) | CN116438303A (pt) |
AU (1) | AU2021236681A1 (pt) |
BR (1) | BR112022018769A2 (pt) |
CA (1) | CA3178847A1 (pt) |
IL (1) | IL296569A (pt) |
MX (1) | MX2022011641A (pt) |
WO (1) | WO2021188830A2 (pt) |
ZA (1) | ZA202211358B (pt) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113699148B (zh) * | 2021-07-15 | 2024-01-09 | 四川大学 | 一种超灵敏抗体检测方法 |
US11814689B2 (en) | 2021-07-21 | 2023-11-14 | Montana State University | Nucleic acid detection using type III CRISPR complex |
CN113817873A (zh) * | 2021-09-30 | 2021-12-21 | 华南理工大学 | 一种用于新冠病毒d614g突变的检测方法及试剂盒 |
US20230167485A1 (en) * | 2021-11-29 | 2023-06-01 | Microsoft Technology Licensing, Llc | Multiplex assay for nucleic acid detection |
CN113913406B (zh) * | 2021-12-14 | 2022-04-08 | 中国人民解放军军事科学院军事医学研究院 | 一种用于检测SARS-CoV-2 69:70del位点的方法 |
CN114236124B (zh) * | 2021-12-16 | 2023-06-09 | 军事科学院军事医学研究院环境医学与作业医学研究所 | 一种基于等温扩增和Cas14a双重放大的检测肌酸激酶同工酶的试剂盒 |
CN114350854B (zh) * | 2022-01-10 | 2023-08-01 | 中国人民解放军军事科学院军事医学研究院 | 一种基于RAA-CRISPR检测SARS-CoV-2 69-70del位点的方法 |
CN114807316B (zh) * | 2022-03-11 | 2023-02-03 | 北京科技大学 | 一种无核酸扩增可视化的rna定量检测方法 |
WO2023201275A1 (en) * | 2022-04-13 | 2023-10-19 | The Board Of Trustees Of The Leland Stanford Junior University | Enzyme kinetics analyses of crispr endonucleases |
WO2023235887A1 (en) * | 2022-06-03 | 2023-12-07 | Tpb Management Llc | Computational multiplexing and application thereof |
WO2024011208A1 (en) | 2022-07-08 | 2024-01-11 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | VIRAL DIAGNOSTIC USING CRISPR RNA COMBINATIONS AND Cas13a ENZYME |
WO2024091905A2 (en) * | 2022-10-27 | 2024-05-02 | University Of Rochester | Compositions, methods and systems for high-fidelity cas13a variants with improved specificity |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6867851B2 (en) * | 1999-11-04 | 2005-03-15 | Regents Of The University Of Minnesota | Scanning of biological samples |
US7593157B2 (en) * | 2004-11-29 | 2009-09-22 | Nikon Corporation | Zoom microscope |
US7813013B2 (en) * | 2006-11-21 | 2010-10-12 | Illumina, Inc. | Hexagonal site line scanning method and system |
US20090162863A1 (en) * | 2007-12-13 | 2009-06-25 | Hitachi High-Technologies Corporation | Nucleic acid detection probe |
US10337051B2 (en) * | 2016-06-16 | 2019-07-02 | The Regents Of The University Of California | Methods and compositions for detecting a target RNA |
WO2020051452A2 (en) * | 2018-09-07 | 2020-03-12 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Hiv or hcv detection with crispr-cas13a |
-
2021
- 2021-03-18 US US17/206,020 patent/US20210348243A1/en active Pending
- 2021-03-18 MX MX2022011641A patent/MX2022011641A/es unknown
- 2021-03-18 KR KR1020227036318A patent/KR20230003470A/ko unknown
- 2021-03-18 WO PCT/US2021/023025 patent/WO2021188830A2/en active Application Filing
- 2021-03-18 EP EP21772507.6A patent/EP4121568A2/en active Pending
- 2021-03-18 JP JP2022556273A patent/JP2023520754A/ja active Pending
- 2021-03-18 BR BR112022018769A patent/BR112022018769A2/pt unknown
- 2021-03-18 CA CA3178847A patent/CA3178847A1/en active Pending
- 2021-03-18 AU AU2021236681A patent/AU2021236681A1/en active Pending
- 2021-03-18 IL IL296569A patent/IL296569A/en unknown
- 2021-03-18 CN CN202180036013.2A patent/CN116438303A/zh active Pending
-
2022
- 2022-10-17 ZA ZA2022/11358A patent/ZA202211358B/en unknown
Also Published As
Publication number | Publication date |
---|---|
KR20230003470A (ko) | 2023-01-06 |
MX2022011641A (es) | 2022-12-08 |
IL296569A (en) | 2022-11-01 |
CA3178847A1 (en) | 2021-09-23 |
AU2021236681A1 (en) | 2022-11-17 |
ZA202211358B (en) | 2024-02-28 |
EP4121568A2 (en) | 2023-01-25 |
CN116438303A (zh) | 2023-07-14 |
WO2021188830A2 (en) | 2021-09-23 |
JP2023520754A (ja) | 2023-05-19 |
US20210348243A1 (en) | 2021-11-11 |
WO2021188830A3 (en) | 2021-11-11 |
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