DK3448990T5 - Fremgangsmåder til modifikation af målnukleinsyrer ved anvendelse af et fusionsmolekyle bestående af guide- og donor-dna, rna-fusionsmolekyle og vektorsystemer, der koder for rna-fusionsmolekylet - Google Patents

Fremgangsmåder til modifikation af målnukleinsyrer ved anvendelse af et fusionsmolekyle bestående af guide- og donor-dna, rna-fusionsmolekyle og vektorsystemer, der koder for rna-fusionsmolekylet Download PDF

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DK3448990T5
DK3448990T5 DK17720040.9T DK17720040T DK3448990T5 DK 3448990 T5 DK3448990 T5 DK 3448990T5 DK 17720040 T DK17720040 T DK 17720040T DK 3448990 T5 DK3448990 T5 DK 3448990T5
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fusion molecule
rna
encooding
moles
guide
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DK17720040.9T
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English (en)
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DK3448990T3 (da
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Leendert W Neuteboom
John A Mcelver
Pinho Barroco Rosa De
Wilde Chris De
Max Fabian Felle
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Basf Plant Science Co Gmbh
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DK17720040.9T 2016-04-29 2017-04-20 Fremgangsmåder til modifikation af målnukleinsyrer ved anvendelse af et fusionsmolekyle bestående af guide- og donor-dna, rna-fusionsmolekyle og vektorsystemer, der koder for rna-fusionsmolekylet DK3448990T5 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP16167773 2016-04-29
EP16167774 2016-04-29
EP17156018 2017-02-14
PCT/EP2017/059331 WO2017186550A1 (en) 2016-04-29 2017-04-20 Improved methods for modification of target nucleic acids

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DK3448990T3 DK3448990T3 (da) 2021-08-30
DK3448990T5 true DK3448990T5 (da) 2021-09-27

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US (5) US11608499B2 (cg-RX-API-DMAC7.html)
EP (5) EP4166661A1 (cg-RX-API-DMAC7.html)
JP (2) JP7184648B2 (cg-RX-API-DMAC7.html)
KR (3) KR20230038804A (cg-RX-API-DMAC7.html)
CN (1) CN109072207B (cg-RX-API-DMAC7.html)
AU (5) AU2017256517B2 (cg-RX-API-DMAC7.html)
CA (1) CA3022475A1 (cg-RX-API-DMAC7.html)
CL (1) CL2018003074A1 (cg-RX-API-DMAC7.html)
DK (1) DK3448990T5 (cg-RX-API-DMAC7.html)
IL (1) IL262409B2 (cg-RX-API-DMAC7.html)
MX (2) MX388092B (cg-RX-API-DMAC7.html)
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Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2853829C (en) 2011-07-22 2023-09-26 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US20150166982A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting pi3k point mutations
EP3177718B1 (en) 2014-07-30 2022-03-16 President and Fellows of Harvard College Cas9 proteins including ligand-dependent inteins
EP3204496A1 (en) 2014-10-10 2017-08-16 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
WO2016073990A2 (en) 2014-11-07 2016-05-12 Editas Medicine, Inc. Methods for improving crispr/cas-mediated genome-editing
US11667911B2 (en) 2015-09-24 2023-06-06 Editas Medicine, Inc. Use of exonucleases to improve CRISPR/CAS-mediated genome editing
SG11201803173VA (en) 2015-10-23 2018-05-30 Harvard College Nucleobase editors and uses thereof
WO2017165826A1 (en) 2016-03-25 2017-09-28 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
EP4047092B1 (en) 2016-04-13 2025-07-30 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
KR20190012229A (ko) 2016-06-01 2019-02-08 케이더블유에스 사아트 에스이 유전자 조작을 위한 하이브리드 핵산 서열
JP7231935B2 (ja) 2016-08-03 2023-03-08 プレジデント アンド フェローズ オブ ハーバード カレッジ アデノシン核酸塩基編集因子およびそれらの使用
CA3033327A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
WO2018071868A1 (en) 2016-10-14 2018-04-19 President And Fellows Of Harvard College Aav delivery of nucleobase editors
WO2018071663A1 (en) * 2016-10-14 2018-04-19 Emendobio Inc. Rna compositions for genome editing
KR20190089175A (ko) * 2016-11-18 2019-07-30 진에딧 인코포레이티드 표적 핵산 변형을 위한 조성물 및 방법
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
US20190352626A1 (en) * 2017-01-30 2019-11-21 KWS SAAT SE & Co. KGaA Repair template linkage to endonucleases for genome engineering
US12390514B2 (en) 2017-03-09 2025-08-19 President And Fellows Of Harvard College Cancer vaccine
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
KR20240116572A (ko) 2017-03-23 2024-07-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
MX2019015188A (es) 2017-06-15 2020-08-03 Univ California Inserciones de adn no virales orientadas.
US11866726B2 (en) 2017-07-14 2024-01-09 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
EP3658573A1 (en) 2017-07-28 2020-06-03 President and Fellows of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace)
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
AU2018352592C1 (en) 2017-10-16 2025-09-25 Beam Therapeutics, Inc. Uses of adenosine base editors
MX2020004325A (es) 2017-10-27 2020-11-09 Univ California Reemplazo dirigido de receptores de células t endógenos.
EP3724214A4 (en) 2017-12-15 2021-09-01 The Broad Institute Inc. SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING
US11268092B2 (en) 2018-01-12 2022-03-08 GenEdit, Inc. Structure-engineered guide RNA
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
CN113286880A (zh) 2018-08-28 2021-08-20 旗舰先锋创新Vi有限责任公司 调控基因组的方法和组合物
US12281338B2 (en) 2018-10-29 2025-04-22 The Broad Institute, Inc. Nucleobase editors comprising GeoCas9 and uses thereof
KR102298105B1 (ko) 2019-01-08 2021-09-03 주식회사 엘지에너지솔루션 스웰링 게이지를 구비한 배터리 모듈 및 이를 포함하는 배터리 팩
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
GB2601617B (en) 2019-03-19 2024-02-21 Broad Inst Inc Methods and compositions for editing nucleotide sequences
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
WO2021175759A1 (en) 2020-03-04 2021-09-10 Basf Se Method for the production of constitutive bacterial promoters conferring low to medium expression
AU2021230545A1 (en) 2020-03-04 2022-09-29 Flagship Pioneering Innovations Vi, Llc Improved methods and compositions for modulating a genome
KR20220149567A (ko) 2020-03-04 2022-11-08 바스프 에스이 이. 콜라이 및 바실루스에서의 발현을 위한 셔틀 벡터
DE112021002672T5 (de) 2020-05-08 2023-04-13 President And Fellows Of Harvard College Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz
CN112111505B (zh) * 2020-09-17 2022-03-15 江南大学 一种氧化葡萄糖酸杆菌中基因敲除的方法
WO2022251179A1 (en) * 2021-05-26 2022-12-01 Inscripta, Inc. Crispr editing in diploid genomes
EP4384616A4 (en) * 2021-08-11 2025-08-27 Univ Leland Stanford Junior HIGH-THROUGHPUT PRECISION GENOME EDITING IN HUMAN CELLS
WO2023064928A2 (en) 2021-10-14 2023-04-20 Arsenal Biosciences, Inc. Immune cells having co-expressed shrnas and logic gate systems
WO2023114872A2 (en) * 2021-12-14 2023-06-22 The Broad Institute, Inc. Reprogrammable fanzor polynucleotides and uses thereof
US20230374480A1 (en) 2022-03-01 2023-11-23 BASF Agricultural Solutions Seed US LLC Cas12a nickases
CN114774420B (zh) * 2022-04-27 2023-09-22 扬州大学 一种水稻增强子及基于CRISPR/Cas9技术评估水稻增强子活性的方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5565350A (en) 1993-12-09 1996-10-15 Thomas Jefferson University Compounds and methods for site directed mutations in eukaryotic cells
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
US6555732B1 (en) 1998-09-14 2003-04-29 Pioneer Hi-Bred International, Inc. Rac-like genes and methods of use
EP1152058A1 (en) 2000-05-03 2001-11-07 Institut Curie Methods and compositions for effecting homologous recombination
DK3284833T3 (da) 2005-08-26 2022-02-07 Dupont Nutrition Biosci Aps Anvendelse af crispr-associerede gener (cas)
AU2014235794A1 (en) * 2013-03-14 2015-10-22 Caribou Biosciences, Inc. Compositions and methods of nucleic acid-targeting nucleic acids
EP3620524A1 (en) 2013-06-17 2020-03-11 The Broad Institute, Inc. Delivery, engineering and optimization of systems, methods and compositions for targeting and modeling diseases and disorders of post mitotic cells
US10563225B2 (en) * 2013-07-26 2020-02-18 President And Fellows Of Harvard College Genome engineering
US10968462B2 (en) 2013-08-12 2021-04-06 BASF Agro B.V. Plants having increased tolerance to herbicides
WO2015092706A1 (en) 2013-12-18 2015-06-25 BASF Agro B.V. Plants having increased tolerance to herbicides
CA2951707A1 (en) * 2014-06-10 2015-12-17 Massachusetts Institute Of Technology Method for gene editing
US20170306306A1 (en) * 2014-10-24 2017-10-26 Life Technologies Corporation Compositions and Methods for Enhancing Homologous Recombination
EP3294879A4 (en) 2015-05-14 2019-02-20 University of Southern California OPTIMIZED GENETIZATION WITH A RECOMBINANT ENDONUCLEASE SYSTEM
WO2017024047A1 (en) 2015-08-03 2017-02-09 Emendobio Inc. Compositions and methods for increasing nuclease induced recombination rate in cells
JP2018532402A (ja) 2015-09-24 2018-11-08 クリスパー セラピューティクス アーゲー Rnaプログラム可能エンドヌクレアーゼの新規のファミリーならびにゲノム編集および他の適用におけるそれらの使用
EP3356520B1 (en) * 2015-10-02 2022-03-23 The U.S.A. as represented by the Secretary, Department of Health and Human Services Lentiviral protein delivery system for rna-guided genome editing
CN106811479B (zh) * 2015-11-30 2019-10-25 中国农业科学院作物科学研究所 利用CRISPR/Cas9系统定点修饰ALS基因获得抗除草剂水稻的系统及其应用
WO2017180711A1 (en) * 2016-04-13 2017-10-19 Editas Medicine, Inc. Grna fusion molecules, gene editing systems, and methods of use thereof
KR20190089175A (ko) * 2016-11-18 2019-07-30 진에딧 인코포레이티드 표적 핵산 변형을 위한 조성물 및 방법

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