DK3419991T3 - Modificerede ribosomale genpromotorer til proteinproduktion i mikroorganismer - Google Patents

Modificerede ribosomale genpromotorer til proteinproduktion i mikroorganismer Download PDF

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Publication number
DK3419991T3
DK3419991T3 DK17713100.0T DK17713100T DK3419991T3 DK 3419991 T3 DK3419991 T3 DK 3419991T3 DK 17713100 T DK17713100 T DK 17713100T DK 3419991 T3 DK3419991 T3 DK 3419991T3
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DK
Denmark
Prior art keywords
microorganisms
protein production
gene promoters
ribosomal gene
modified ribosomal
Prior art date
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DK17713100.0T
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Danish (da)
English (en)
Inventor
Cristina Bongiorni
Marc Kolkman
Chris Leeflang
Virgil Arthur Rhodius
Kimmenade Anita Van
Original Assignee
Danisco Us Inc
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Publication of DK3419991T3 publication Critical patent/DK3419991T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK17713100.0T 2016-03-04 2017-03-06 Modificerede ribosomale genpromotorer til proteinproduktion i mikroorganismer DK3419991T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662304061P 2016-03-04 2016-03-04
PCT/US2017/020913 WO2017152169A1 (en) 2016-03-04 2017-03-06 Engineered ribosomal promoters for protein production in microorganisms

Publications (1)

Publication Number Publication Date
DK3419991T3 true DK3419991T3 (da) 2023-01-16

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Family Applications (1)

Application Number Title Priority Date Filing Date
DK17713100.0T DK3419991T3 (da) 2016-03-04 2017-03-06 Modificerede ribosomale genpromotorer til proteinproduktion i mikroorganismer

Country Status (8)

Country Link
US (2) US11447782B2 (https=)
EP (2) EP4095152A3 (https=)
JP (2) JP7103948B2 (https=)
KR (1) KR20180117166A (https=)
CN (1) CN109071615A (https=)
DK (1) DK3419991T3 (https=)
FI (1) FI3419991T3 (https=)
WO (1) WO2017152169A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019055261A1 (en) * 2017-09-13 2019-03-21 Danisco Us Inc MODIFIED 5 'NON-TRANSLATED REGION (UTR) SEQUENCES FOR INCREASED PROTEIN PRODUCTION IN BACILLUS
CN108441489B (zh) * 2018-04-09 2023-04-14 潍坊康地恩生物科技有限公司 一种蛋白生产方法及高产碱性蛋白酶的枯草芽孢杆菌
WO2020046613A1 (en) 2018-08-30 2020-03-05 Danisco Us Inc Compositions comprising a lipolytic enzyme variant and methods of use thereof
CN113366108B (zh) * 2018-11-28 2024-11-22 丹尼斯科美国公司 新颖启动子序列及其增强芽孢杆菌属细胞蛋白生产的方法
WO2021030267A1 (en) 2019-08-14 2021-02-18 Danisco Us Inc Compositions and methods for increased protein production in bacillus licheniformis
US20230049452A1 (en) 2020-01-13 2023-02-16 Danisco Us Inc Compositions comprising a lipolytic enzyme variant and methods of use thereof
CN119630280A (zh) 2022-05-14 2025-03-14 诺维信公司 用于预防、处理、抑制和/或消除植物病原性侵染和感染的组合物和方法
KR20250054813A (ko) 2022-09-02 2025-04-23 다니스코 유에스 인크. 그람 양성 세포에서 단백질 생산을 향상시키는 신규한 프로모터 및 5'-비번역 영역 돌연변이

Family Cites Families (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4559300A (en) 1983-01-18 1985-12-17 Eli Lilly And Company Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5264366A (en) 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
US5182204A (en) 1984-05-29 1993-01-26 Genencor International, Inc. Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5364770A (en) 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
FR2625295B1 (fr) 1987-12-24 1990-04-13 Gaz De France Procede et appareil destines a assurer la combustion etagee d'un melange combustible-comburant diminuant la production d'oxydes d'azote
EP0351029B1 (en) 1988-07-15 2002-03-06 Genencor International, Inc. Novel glucose isomerase enzymes and their use
WO1992010581A1 (en) 1990-12-10 1992-06-25 Genencor International, Inc. IMPROVED SACCHARIFICATION OF CELLULOSE BY CLONING AND AMPLIFICATION OF THE β-GLUCOSIDASE GENE OF TRICHODERMA REESEI
US6509183B1 (en) 1993-02-25 2003-01-21 Kao Corporation DNA fragment containing gene for alkaline pullulanase
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
US6255115B1 (en) 1997-04-07 2001-07-03 Unilever Patent Holdings Bv Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus
AR016969A1 (es) 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
US6562612B2 (en) 1997-11-19 2003-05-13 Genencor International, Inc. Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
US6287839B1 (en) 1997-11-19 2001-09-11 Genencor International, Inc. Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
US6268328B1 (en) 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
US7718411B1 (en) 2004-08-05 2010-05-18 Danisco Us Inc. Trichoderma reesei G/11 xylanases with improved stability
FI108728B (fi) 1999-10-12 2002-03-15 Carbozyme Oy Menetelmä perheen G/11 ksylanaasien stabiilisuuden parantamiseksi ja optimaalisen pH-alueen muuttamiseksi
US6509185B1 (en) 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor
EP1250423B1 (en) 2000-01-12 2008-09-03 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
AU785107C (en) 2000-03-08 2007-05-03 Dupont Nutrition Biosciences Aps Xylanase variants having altered sensitivity to xylanase inhibitors
KR100390565B1 (ko) 2000-06-20 2003-07-07 주식회사 씨티씨바이오 만난아제를 생산하는 신규한 바실러스 속 ｗｌ-1 균주
CA2418317A1 (en) 2000-08-11 2002-02-21 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
EP1444246B1 (en) 2001-10-26 2015-12-30 Danisco US Inc. Trichoderma reesei phytase enzymes, nucleic acids encoding such phytase enzymes, vectors and host cells incorporating same and methods of making and using same
EP1438417B1 (en) 2001-10-26 2014-05-14 Danisco US Inc. Phytase enzymes, nucleic acid sequences encoding phytase enzymes and vectors and host cells incorporating same
MXPA04006811A (es) 2002-01-16 2004-10-11 Genencor Int Variantes de proteasa substituidas con multiplos.
KR20040104581A (ko) 2002-04-22 2004-12-10 이 아이 듀폰 디 네모아 앤드 캄파니 유전공학용 프로모터 및 플라스미드 시스템
CN101792749B (zh) 2002-08-12 2012-09-05 金克克国际有限公司 大肠杆菌AppA肌醇六磷酸酶突变体
EP1618183B1 (en) 2003-04-29 2014-11-19 Danisco US Inc. Novel bacillus 029cel cellulase
CN101374852B (zh) 2003-04-30 2012-06-06 金克克国际有限公司 杆菌mHKcel纤维素酶
CN101300335A (zh) 2003-04-30 2008-11-05 金克克国际有限公司 新颖的杆菌BagCel纤维素酶
EP2354222A1 (en) 2004-03-25 2011-08-10 Genencor International, Inc. Cellulase fusion protein and heterologous cellulase fusion construct encoding the same
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
WO2006038128A2 (en) 2004-10-04 2006-04-13 Danisco A/S Citrobacter freundii phytase and homologues
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
CA2591221A1 (en) 2004-12-23 2006-07-06 Genencor International, Inc. Neutral cellulase catalytic core and method of producing same
RU2509152C2 (ru) 2006-07-18 2014-03-10 ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН Cпособ мытья посуды
US7968691B2 (en) 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
MX2009003467A (es) 2006-10-06 2009-04-14 Danisco Us Inc Genencor Div Composiciones enzimaticas libres de celulasa y celulas hospederas para producir las mismas.
AU2008209720C1 (en) 2007-01-30 2016-06-16 Novozymes A/S Polypeptides having phytase activty and polynucleotides encoding same
CN101688192A (zh) 2007-02-07 2010-03-31 丹尼斯科美国公司 用植酸酶和α-淀粉酶进行淀粉水解
US20080293607A1 (en) 2007-03-09 2008-11-27 Jones Brian E Alkaliphilic Bacillus Species alpha-Amylase Variants, Compositions Comprising alpha-Amylase Variants, And Methods of Use
RU2009139059A (ru) 2007-03-23 2011-04-27 ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН (US) Улучшенное производство амилазы посредством присоединения к n-концу зрелого белка амилазы
EP2155869A2 (en) 2007-05-30 2010-02-24 Danisco US, INC., Genencor Division Variants of an alpha-amylase with improved production levels in fermentation processes
WO2008153805A2 (en) 2007-05-30 2008-12-18 Danisco Us, Inc., Genencor Division Improved variants of the bacillus licheniformis alpha-amylase
MX344810B (es) 2007-06-08 2017-01-09 Danisco Us Inc Genencor Div * Sistema de expresion de celulasa heterologa y homologa.
CN101796195A (zh) 2007-09-07 2010-08-04 丹尼斯科美国公司 β-葡糖苷酶增强的丝状真菌全纤维素酶组合物和使用方法
EP3323889B1 (en) 2007-11-20 2020-03-25 Danisco US Inc. Glucoamylase variants with altered properties
EP2283124B1 (en) 2008-04-18 2016-05-25 Danisco US Inc. Buttiauxella sp. phytase variants
CN102057040A (zh) 2008-06-06 2011-05-11 丹尼斯科美国公司 具有改良特性的嗜热脂肪土芽孢杆菌α-淀粉酶(AMYS)变体
BRPI0915606A2 (pt) 2008-06-06 2021-03-09 Danisco Us Inc. Alfa-amilases variantes de bacillus subtilis e métodos de uso das mesmas
EP2288698B1 (en) 2008-06-06 2016-12-21 Danisco US Inc. Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials
AR074105A1 (es) 2008-11-11 2010-12-22 Danisco Us Inc Composiciones y metodos que comprenden variantes de subtilisina
CN102209778B (zh) 2008-11-11 2014-10-15 丹尼斯科美国公司 包含丝氨酸蛋白酶变体的组合物和方法
ES2602792T3 (es) 2008-12-23 2017-02-22 Dupont Nutrition Biosciences Aps Polipéptidos con actividad de xilanasa
US8530218B2 (en) 2009-04-24 2013-09-10 Danisco Us Inc. Proteases with modified pro regions
GB0922467D0 (en) 2009-04-24 2010-02-03 Danisco Feed supplement
EP3473711B1 (en) 2009-05-19 2020-11-04 DuPont Nutrition Biosciences ApS Amylase polypeptides
WO2010141779A1 (en) 2009-06-03 2010-12-09 Danisco Us Inc. Cellulase variants with improved expression, activity and/or stability, and use thereof
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
US20110171718A1 (en) 2009-07-31 2011-07-14 Danisco Us Inc. Proteases With Modified Pre-Pro Regions
KR20110046205A (ko) 2009-10-28 2011-05-04 김도위 알레르겐 진단을 위한 바늘 구비형 피부 진단용 시험기
US8728790B2 (en) 2009-12-09 2014-05-20 Danisco Us Inc. Compositions and methods comprising protease variants
EP2712363A1 (en) 2011-04-29 2014-04-02 Danisco US Inc. Detergent compositions containing geobacillus tepidamans mannanase and methods of use thereof
WO2012149333A1 (en) 2011-04-29 2012-11-01 Danisco Us Inc. Detergent compositions containing bacillus sp. mannanase and methods of use thereof
BR112014005873B1 (pt) 2011-09-14 2021-11-16 Dupont Nutrition Biosciences Aps Composição, uso de uma composição, método de alteração da filtrabilidade, método de redução da pressão acumulada, método para a produção de um alimento, método para a produção de uma pasta de brassagem e método para a produção de um biocombustível
WO2013086219A1 (en) * 2011-12-09 2013-06-13 Danisco Us Inc. Ribosomal promotors from b. subtilis for protein production in microorganisms
WO2013127069A1 (en) 2012-02-29 2013-09-06 Danisco Us Inc. Thermostable xylanase from thermobifida fusca and methods of use thereof
US9685619B2 (en) 2012-07-09 2017-06-20 Yeda Research And Development Co. Ltd. Logic circuits with plug and play solid-state molecular chips
BR112015012904A2 (pt) 2012-12-07 2017-09-12 Danisco Us Inc composições e métodos de uso
WO2014099525A1 (en) 2012-12-21 2014-06-26 Danisco Us Inc. Paenibacillus curdlanolyticus amylase, and methods of use, thereof
GB201401680D0 (en) 2014-01-31 2014-03-19 Dupont Nutrition Biosci Aps Compositions and methods comprising a xylanase enzyme variant

Also Published As

Publication number Publication date
JP2022116087A (ja) 2022-08-09
EP4095152A2 (en) 2022-11-30
US11447782B2 (en) 2022-09-20
EP4095152A3 (en) 2022-12-28
EP3419991B1 (en) 2022-10-26
EP3419991A1 (en) 2019-01-02
US12077762B1 (en) 2024-09-03
FI3419991T3 (fi) 2023-01-31
US20190085341A1 (en) 2019-03-21
JP2019511218A (ja) 2019-04-25
CN109071615A (zh) 2018-12-21
WO2017152169A1 (en) 2017-09-08
JP7103948B2 (ja) 2022-07-20
KR20180117166A (ko) 2018-10-26

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