DK3419991T3 - Modificerede ribosomale genpromotorer til proteinproduktion i mikroorganismer - Google Patents

Modificerede ribosomale genpromotorer til proteinproduktion i mikroorganismer Download PDF

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Publication number
DK3419991T3
DK3419991T3 DK17713100.0T DK17713100T DK3419991T3 DK 3419991 T3 DK3419991 T3 DK 3419991T3 DK 17713100 T DK17713100 T DK 17713100T DK 3419991 T3 DK3419991 T3 DK 3419991T3
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DK
Denmark
Prior art keywords
microorganisms
protein production
gene promoters
ribosomal gene
modified ribosomal
Prior art date
Application number
DK17713100.0T
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Danish (da)
English (en)
Inventor
Cristina Bongiorni
Marc Kolkman
Chris Leeflang
Virgil Arthur Rhodius
Kimmenade Anita Van
Original Assignee
Danisco Us Inc
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Publication of DK3419991T3 publication Critical patent/DK3419991T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK17713100.0T 2016-03-04 2017-03-06 Modificerede ribosomale genpromotorer til proteinproduktion i mikroorganismer DK3419991T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662304061P 2016-03-04 2016-03-04
PCT/US2017/020913 WO2017152169A1 (en) 2016-03-04 2017-03-06 Engineered ribosomal promoters for protein production in microorganisms

Publications (1)

Publication Number Publication Date
DK3419991T3 true DK3419991T3 (da) 2023-01-16

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ID=58402134

Family Applications (1)

Application Number Title Priority Date Filing Date
DK17713100.0T DK3419991T3 (da) 2016-03-04 2017-03-06 Modificerede ribosomale genpromotorer til proteinproduktion i mikroorganismer

Country Status (8)

Country Link
US (2) US11447782B2 (enExample)
EP (2) EP4095152A3 (enExample)
JP (2) JP7103948B2 (enExample)
KR (1) KR20180117166A (enExample)
CN (1) CN109071615A (enExample)
DK (1) DK3419991T3 (enExample)
FI (1) FI3419991T3 (enExample)
WO (1) WO2017152169A1 (enExample)

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JP7218985B2 (ja) * 2017-09-13 2023-02-07 ダニスコ・ユーエス・インク バチルス属（Ｂａｃｉｌｌｕｓ）におけるタンパク質産生の増加のための改変５’－非翻訳領域（ＵＴＲ）配列
CN108441489B (zh) * 2018-04-09 2023-04-14 潍坊康地恩生物科技有限公司 一种蛋白生产方法及高产碱性蛋白酶的枯草芽孢杆菌
WO2020046613A1 (en) 2018-08-30 2020-03-05 Danisco Us Inc Compositions comprising a lipolytic enzyme variant and methods of use thereof
KR20210096629A (ko) * 2018-11-28 2021-08-05 다니스코 유에스 인크. 바실러스 세포에서의 향상된 단백질 생산을 위한 신규 프로모터 서열 및 이의 방법
EP4031560A1 (en) 2019-08-14 2022-07-27 Danisco US Inc Compositions and methods for increased protein production in bacillus licheniformis
EP4090727A1 (en) 2020-01-13 2022-11-23 Danisco US Inc. Compositions comprising a lipolytic enzyme variant and methods of use thereof
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WO2024050503A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Novel promoter and 5'-untranslated region mutations enhancing protein production in gram-positive cells

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Also Published As

Publication number Publication date
KR20180117166A (ko) 2018-10-26
JP7103948B2 (ja) 2022-07-20
CN109071615A (zh) 2018-12-21
EP3419991B1 (en) 2022-10-26
WO2017152169A1 (en) 2017-09-08
US11447782B2 (en) 2022-09-20
US12077762B1 (en) 2024-09-03
EP3419991A1 (en) 2019-01-02
EP4095152A3 (en) 2022-12-28
FI3419991T3 (fi) 2023-01-31
EP4095152A2 (en) 2022-11-30
JP2019511218A (ja) 2019-04-25
US20190085341A1 (en) 2019-03-21
JP2022116087A (ja) 2022-08-09

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