KR20180117166A - 미생물에서의 단백질 생산을 위한 유전자 조작 리보솜 프로모터 - Google Patents

미생물에서의 단백질 생산을 위한 유전자 조작 리보솜 프로모터 Download PDF

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KR20180117166A
KR20180117166A KR1020187027921A KR20187027921A KR20180117166A KR 20180117166 A KR20180117166 A KR 20180117166A KR 1020187027921 A KR1020187027921 A KR 1020187027921A KR 20187027921 A KR20187027921 A KR 20187027921A KR 20180117166 A KR20180117166 A KR 20180117166A
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seq
promoter
nucleic acid
pro
sequence
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크리스티나 본조르니
마르크 콜크만
크리스 레플랑
버질 아서 로디우스
킴메나데 아니타 판
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다니스코 유에스 인크.
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001Alpha-amylase (3.2.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21062Subtilisin (3.4.21.62)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020187027921A 2016-03-04 2017-03-06 미생물에서의 단백질 생산을 위한 유전자 조작 리보솜 프로모터 Withdrawn KR20180117166A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662304061P 2016-03-04 2016-03-04
US62/304,061 2016-03-04
PCT/US2017/020913 WO2017152169A1 (en) 2016-03-04 2017-03-06 Engineered ribosomal promoters for protein production in microorganisms

Publications (1)

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KR20180117166A true KR20180117166A (ko) 2018-10-26

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KR1020187027921A Withdrawn KR20180117166A (ko) 2016-03-04 2017-03-06 미생물에서의 단백질 생산을 위한 유전자 조작 리보솜 프로모터

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US (2) US11447782B2 (enExample)
EP (2) EP4095152A3 (enExample)
JP (2) JP7103948B2 (enExample)
KR (1) KR20180117166A (enExample)
CN (1) CN109071615A (enExample)
DK (1) DK3419991T3 (enExample)
FI (1) FI3419991T3 (enExample)
WO (1) WO2017152169A1 (enExample)

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CN108441489B (zh) * 2018-04-09 2023-04-14 潍坊康地恩生物科技有限公司 一种蛋白生产方法及高产碱性蛋白酶的枯草芽孢杆菌
EP3833731A1 (en) 2018-08-30 2021-06-16 Danisco US Inc. Compositions comprising a lipolytic enzyme variant and methods of use thereof
US11781147B2 (en) 2018-11-28 2023-10-10 Danisco Us Inc. Promoter sequences and methods thereof for enhanced protein production in Bacillus cells
KR20220041928A (ko) 2019-08-14 2022-04-01 다니스코 유에스 인크. 바실러스 리체니포르미스에서 단백질 생산을 증가시키기 위한 조성물 및 방법
WO2021146255A1 (en) 2020-01-13 2021-07-22 Danisco Us Inc Compositions comprising a lipolytic enzyme variant and methods of use thereof
US20250311734A1 (en) 2022-05-14 2025-10-09 Novozymes A/S Compositions and Methods for Preventing, Treating, Suppressing and/or Eliminatting Phytopathogenic Infestations and Infections
US20260092297A1 (en) 2022-09-02 2026-04-02 Danisco Us Inc. Novel promoter and 5'-untranslated region mutations enhancing protein production in gram-positive cells

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Publication number Publication date
EP3419991B1 (en) 2022-10-26
EP3419991A1 (en) 2019-01-02
WO2017152169A1 (en) 2017-09-08
JP2019511218A (ja) 2019-04-25
US20190085341A1 (en) 2019-03-21
US11447782B2 (en) 2022-09-20
JP7103948B2 (ja) 2022-07-20
EP4095152A2 (en) 2022-11-30
DK3419991T3 (da) 2023-01-16
CN109071615A (zh) 2018-12-21
JP2022116087A (ja) 2022-08-09
EP4095152A3 (en) 2022-12-28
FI3419991T3 (fi) 2023-01-31
US12077762B1 (en) 2024-09-03

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