JP7103948B2 - 微生物におけるタンパク質産生のための遺伝子操作されたリボソームプロモーター - Google Patents

微生物におけるタンパク質産生のための遺伝子操作されたリボソームプロモーター Download PDF

Info

Publication number
JP7103948B2
JP7103948B2 JP2018546634A JP2018546634A JP7103948B2 JP 7103948 B2 JP7103948 B2 JP 7103948B2 JP 2018546634 A JP2018546634 A JP 2018546634A JP 2018546634 A JP2018546634 A JP 2018546634A JP 7103948 B2 JP7103948 B2 JP 7103948B2
Authority
JP
Japan
Prior art keywords
seq
promoter
nucleic acid
sequence
poi
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2018546634A
Other languages
English (en)
Japanese (ja)
Other versions
JP2019511218A5 (enExample
JP2019511218A (ja
Inventor
ボンジョルニ、クリスティーナ
コルクマン、マルク
リーフラン、クリス
アーサー ロディアス、ヴァージル
ヴァン・キンメネード、アニータ
Original Assignee
ダニスコ・ユーエス・インク
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ダニスコ・ユーエス・インク filed Critical ダニスコ・ユーエス・インク
Publication of JP2019511218A publication Critical patent/JP2019511218A/ja
Publication of JP2019511218A5 publication Critical patent/JP2019511218A5/ja
Priority to JP2022081023A priority Critical patent/JP2022116087A/ja
Application granted granted Critical
Publication of JP7103948B2 publication Critical patent/JP7103948B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2018546634A 2016-03-04 2017-03-06 微生物におけるタンパク質産生のための遺伝子操作されたリボソームプロモーター Active JP7103948B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022081023A JP2022116087A (ja) 2016-03-04 2022-05-17 微生物におけるタンパク質産生のための遺伝子操作されたリボソームプロモーター

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662304061P 2016-03-04 2016-03-04
US62/304,061 2016-03-04
PCT/US2017/020913 WO2017152169A1 (en) 2016-03-04 2017-03-06 Engineered ribosomal promoters for protein production in microorganisms

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2022081023A Division JP2022116087A (ja) 2016-03-04 2022-05-17 微生物におけるタンパク質産生のための遺伝子操作されたリボソームプロモーター

Publications (3)

Publication Number Publication Date
JP2019511218A JP2019511218A (ja) 2019-04-25
JP2019511218A5 JP2019511218A5 (enExample) 2020-04-16
JP7103948B2 true JP7103948B2 (ja) 2022-07-20

Family

ID=58402134

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2018546634A Active JP7103948B2 (ja) 2016-03-04 2017-03-06 微生物におけるタンパク質産生のための遺伝子操作されたリボソームプロモーター
JP2022081023A Pending JP2022116087A (ja) 2016-03-04 2022-05-17 微生物におけるタンパク質産生のための遺伝子操作されたリボソームプロモーター

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2022081023A Pending JP2022116087A (ja) 2016-03-04 2022-05-17 微生物におけるタンパク質産生のための遺伝子操作されたリボソームプロモーター

Country Status (8)

Country Link
US (2) US11447782B2 (enExample)
EP (2) EP4095152A3 (enExample)
JP (2) JP7103948B2 (enExample)
KR (1) KR20180117166A (enExample)
CN (1) CN109071615A (enExample)
DK (1) DK3419991T3 (enExample)
FI (1) FI3419991T3 (enExample)
WO (1) WO2017152169A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210032639A1 (en) * 2017-09-13 2021-02-04 Danisco Us Inc. Modified 5'-untranslated region (utr) sequences for increased protein production in bacillus
CN108441489B (zh) * 2018-04-09 2023-04-14 潍坊康地恩生物科技有限公司 一种蛋白生产方法及高产碱性蛋白酶的枯草芽孢杆菌
EP3833731A1 (en) 2018-08-30 2021-06-16 Danisco US Inc. Compositions comprising a lipolytic enzyme variant and methods of use thereof
US11781147B2 (en) 2018-11-28 2023-10-10 Danisco Us Inc. Promoter sequences and methods thereof for enhanced protein production in Bacillus cells
KR20220041928A (ko) 2019-08-14 2022-04-01 다니스코 유에스 인크. 바실러스 리체니포르미스에서 단백질 생산을 증가시키기 위한 조성물 및 방법
WO2021146255A1 (en) 2020-01-13 2021-07-22 Danisco Us Inc Compositions comprising a lipolytic enzyme variant and methods of use thereof
US20250311734A1 (en) 2022-05-14 2025-10-09 Novozymes A/S Compositions and Methods for Preventing, Treating, Suppressing and/or Eliminatting Phytopathogenic Infestations and Infections
US20260092297A1 (en) 2022-09-02 2026-04-02 Danisco Us Inc. Novel promoter and 5'-untranslated region mutations enhancing protein production in gram-positive cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015500032A (ja) 2011-12-09 2015-01-05 ダニスコ・ユーエス・インク 微生物におけるタンパク質産生のための、Ｂ．ズブチリス（Ｂ．ｓｕｂｔｉｌｉｓ）からのリボソームプロモーター

Family Cites Families (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4559300A (en) 1983-01-18 1985-12-17 Eli Lilly And Company Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5264366A (en) 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
US5182204A (en) 1984-05-29 1993-01-26 Genencor International, Inc. Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5364770A (en) 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
FR2625295B1 (fr) 1987-12-24 1990-04-13 Gaz De France Procede et appareil destines a assurer la combustion etagee d'un melange combustible-comburant diminuant la production d'oxydes d'azote
EP0351029B1 (en) 1988-07-15 2002-03-06 Genencor International, Inc. Novel glucose isomerase enzymes and their use
DK0562003T4 (en) 1990-12-10 2015-07-13 Danisco Us Inc Improved saccharification of cellulose by cloning and amplification of.-Glucosidase gene from Tricodermareesei
WO1994019468A1 (en) 1993-02-25 1994-09-01 Kao Corporation Dna fragment containing gene for alkaline pullulanase
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
JP4307563B2 (ja) 1997-04-07 2009-08-05 ユニリーバー・ナームローゼ・ベンノートシャープ 糸状菌、特にアスペルギルス属に属する糸状菌のアグロバクテリウム媒介性形質転換
AR016969A1 (es) 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
US6562612B2 (en) 1997-11-19 2003-05-13 Genencor International, Inc. Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
US6287839B1 (en) 1997-11-19 2001-09-11 Genencor International, Inc. Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
US6268328B1 (en) 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
US7718411B1 (en) 2004-08-05 2010-05-18 Danisco Us Inc. Trichoderma reesei G/11 xylanases with improved stability
FI108728B (fi) 1999-10-12 2002-03-15 Carbozyme Oy Menetelmä perheen G/11 ksylanaasien stabiilisuuden parantamiseksi ja optimaalisen pH-alueen muuttamiseksi
US6509185B1 (en) 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor
AU2663801A (en) 2000-01-12 2001-07-24 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
DK1263941T3 (da) 2000-03-08 2011-03-14 Danisco Xylanasevarianter med ændret sensitivitet overfor xylanaseinhibitorer
KR100390565B1 (ko) 2000-06-20 2003-07-07 주식회사 씨티씨바이오 만난아제를 생산하는 신규한 바실러스 속 ｗｌ-1 균주
US20020182734A1 (en) 2000-08-11 2002-12-05 Diaz-Torres Maria R. Bacillus transformation, transformants and mutant libraries
AU2002334950A1 (en) 2001-10-26 2003-05-12 Genencor International, Inc. T. reesei phytase enzymes, polynucleides encoding the enzymes, vectors and host cells thereof, and methods of using
DK1438417T3 (da) 2001-10-26 2014-08-04 Danisco Us Inc Phytase-enzymer, nukleinsyresekvenser, der koder for fytase-enzymer ogvektorer og værtsceller, der inkorporerer disse
WO2003062380A2 (en) 2002-01-16 2003-07-31 Genencor International, Inc. Multiply-substituted protease variants
US8192959B2 (en) 2002-04-22 2012-06-05 Genencor International Inc Promoter and plasmid system for genetic engineering
CA2495660A1 (en) 2002-08-12 2004-02-19 Genencor International, Inc. Mutant e. coli appa phytase enzymes
DK1618183T3 (en) 2003-04-29 2015-02-23 Danisco Us Inc New Bacillus cellulase 029cel
JP4757191B2 (ja) 2003-04-30 2011-08-24 ジェネンコー・インターナショナル・インク 新規なバチルスｍＨＫｃｅｌセルラーゼ
CA2523722A1 (en) 2003-04-30 2004-11-18 Genencor International, Inc. Novel bacillus bagcel cellulase
BRPI0509212A (pt) 2004-03-25 2007-08-28 Genencor Int proteìna de fusão de celulase e construto de fusão de celulase heterólogo codificando a mesma
WO2006038128A2 (en) 2004-10-04 2006-04-13 Danisco A/S Citrobacter freundii phytase and homologues
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
DK1828380T3 (da) 2004-12-23 2011-03-14 Genencor Int Neutral cellulasekatalysatorkerne og fremgangsmåde til fremstilling heraf
WO2008010925A2 (en) 2006-07-18 2008-01-24 Danisco Us, Inc., Genencor Division Protease variants active over a broad temperature range
US7968691B2 (en) 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
BRPI0718226B1 (pt) 2006-10-06 2020-10-27 Danisco Us Inc., Genencor Division célula hospedeira de bacillus sp. recombinante, cultura da mesma e métodos de produção da referida célula e de proteína aditivo para detergentes
EP2109356A2 (en) 2007-01-30 2009-10-21 Novozymes A/S Polypeptides having phytase activty and polynucleotides encoding same
PL2118276T3 (pl) 2007-02-07 2016-01-29 Danisco Us Inc Hydroliza skrobi za pomocą fitazy z alfa-amylazą
WO2008112459A2 (en) 2007-03-09 2008-09-18 Danisco Us Inc., Genencor Division Alkaliphilic bacillus species a-amylase variants, compositions comprising a-amylase variants, and methods of use
DK2132316T3 (da) 2007-03-23 2015-06-22 Danisco Us Inc Forøget amylaseproduktion ved n-terminal addition til modent amylaseprotein
EP2155869A2 (en) 2007-05-30 2010-02-24 Danisco US, INC., Genencor Division Variants of an alpha-amylase with improved production levels in fermentation processes
US20110033882A1 (en) 2007-05-30 2011-02-10 Wolfgang Aehle Variants of the bacillus licheniformis alpha-amylase
CA2693084A1 (en) 2007-06-08 2008-12-18 Benjamin S. Bower Heterologous and homologous cellulase expression system
CN101796195A (zh) 2007-09-07 2010-08-04 丹尼斯科美国公司 β-葡糖苷酶增强的丝状真菌全纤维素酶组合物和使用方法
DK3323889T3 (da) 2007-11-20 2020-06-29 Danisco Us Inc Glucoamylasevarianter med ændrede egenskaber
WO2009129489A2 (en) 2008-04-18 2009-10-22 Danisco Us Inc., Genencor Division Buttiauxella sp. phytase variants
WO2009149419A2 (en) 2008-06-06 2009-12-10 Danisco Us Inc. Variant alpha-amylases from bacillus subtilis and methods of use, thereof
MX2010013099A (es) 2008-06-06 2010-12-21 Danisco Inc Composiciones y metodos que comprenden variantes de celulasa con afinidad reducida a materiales no celulosicos.
WO2009149130A2 (en) 2008-06-06 2009-12-10 Danisco Us Inc. Geobacillus stearothermophilus alpha-amylase (amys) variants with improved properties
US20100192985A1 (en) 2008-11-11 2010-08-05 Wolfgang Aehle Compositions and methods comprising serine protease variants
WO2010056635A1 (en) 2008-11-11 2010-05-20 Danisco Us Inc. Compositions and methods comprising a subtilisin variant
DK2382310T3 (en) 2008-12-23 2016-12-12 Dupont Nutrition Biosci Aps POLYPEPTIDES HAVING xylanase activity
GB0922467D0 (en) 2009-04-24 2010-02-03 Danisco Feed supplement
WO2010123754A1 (en) 2009-04-24 2010-10-28 Danisco Us Inc. Proteases with modified pro regions
EP2432875B1 (en) 2009-05-19 2016-09-07 DuPont Nutrition Biosciences ApS Amylase polypeptides
KR20120062643A (ko) 2009-06-03 2012-06-14 다니스코 유에스 인크. 향상된 발현, 활성 및/또는 안정성을 갖는 셀룰라아제 변이체, 및 이의 용도
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
EP2459714A1 (en) 2009-07-31 2012-06-06 Danisco US Inc. Proteases with modified pre-pro regions
KR20110046205A (ko) 2009-10-28 2011-05-04 김도위 알레르겐 진단을 위한 바늘 구비형 피부 진단용 시험기
JP5863666B2 (ja) 2009-12-09 2016-02-17 ダニスコ・ユーエス・インク プロテアーゼ変異体を含む組成物及び方法
US20140073548A1 (en) 2011-04-29 2014-03-13 Danisco Us Inc. Detergent compositions containing bacillus sp. mannanase and methods of use thereof
BR112013027209A2 (pt) 2011-04-29 2016-11-29 Danisco Us Inc "polipeptídeo recombinante, composição detergente contendo mananase de geobacillus tepidamans, método para limpeza de produto têxtil, vetor de expressão e célula hospedeira"
JP6084617B2 (ja) 2011-09-14 2017-02-22 デュポン ニュートリション バイオサイエンシーズ エーピーエス 酵素
WO2013127069A1 (en) 2012-02-29 2013-09-06 Danisco Us Inc. Thermostable xylanase from thermobifida fusca and methods of use thereof
EP2870644A2 (en) 2012-07-09 2015-05-13 Yeda Research and Development Co. Ltd. Logic circuits with plug and play solid-state molecular chips
EP2928911A2 (en) 2012-12-07 2015-10-14 Danisco US Inc. Compositions of beta-mannanase and methods of use
WO2014099525A1 (en) 2012-12-21 2014-06-26 Danisco Us Inc. Paenibacillus curdlanolyticus amylase, and methods of use, thereof
GB201401680D0 (en) 2014-01-31 2014-03-19 Dupont Nutrition Biosci Aps Compositions and methods comprising a xylanase enzyme variant

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015500032A (ja) 2011-12-09 2015-01-05 ダニスコ・ユーエス・インク 微生物におけるタンパク質産生のための、Ｂ．ズブチリス（Ｂ．ｓｕｂｔｉｌｉｓ）からのリボソームプロモーター

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
EMBO J., 2004, 23(22), pp.4473-4483
J. Gen. Appl. Microbiol., 2006, 52(2), pp.119-124

Also Published As

Publication number Publication date
EP3419991B1 (en) 2022-10-26
EP3419991A1 (en) 2019-01-02
WO2017152169A1 (en) 2017-09-08
JP2019511218A (ja) 2019-04-25
US20190085341A1 (en) 2019-03-21
US11447782B2 (en) 2022-09-20
EP4095152A2 (en) 2022-11-30
DK3419991T3 (da) 2023-01-16
CN109071615A (zh) 2018-12-21
JP2022116087A (ja) 2022-08-09
EP4095152A3 (en) 2022-12-28
FI3419991T3 (fi) 2023-01-31
US12077762B1 (en) 2024-09-03
KR20180117166A (ko) 2018-10-26

Similar Documents

Publication Publication Date Title
JP6378089B2 (ja) 微生物におけるタンパク質産生のための、Ｂ．ズブチリス（Ｂ．ｓｕｂｔｉｌｉｓ）からのリボソームプロモーター
US12077762B1 (en) Engineered ribosomal promoters for protein production in microorganisms
US12448626B2 (en) Compositions and methods for increased protein production in Bacillus licheniformis
KR20200047668A (ko) 바실러스에서 증가된 단백질 생산을 위한 변형된 5'-비번역 영역(utr) 서열
EP3259358B1 (en) Enhanced protein expression
EP3380496B1 (en) Enhanced protein production and methods thereof
JP2023524334A (ja) バチルス・リケニフォルミス（ｂａｃｉｌｌｕｓ ｌｉｃｈｅｎｉｆｏｒｍｉｓ）における強化したタンパク質産生のための組成物及び方法
JP2021510070A (ja) タンパク質の産生増加のための変異及び遺伝子改変バチルス属（ｂａｃｉｌｌｕｓ）細胞、並びにその方法
CN107250371A (zh) 增强的蛋白质表达
JP2025502174A (ja) グラム陽性細菌細胞におけるタンパク質産生を強化するための組成物及び方法
JP7656603B2 (ja) バチルス属（ｂａｃｉｌｌｕｓ）細胞における強化されたタンパク質産生のための組成物及び方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200304

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20200304

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20210330

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210628

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210830

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20220118

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220517

C60 Trial request (containing other claim documents, opposition documents)

Free format text: JAPANESE INTERMEDIATE CODE: C60

Effective date: 20220517

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20220524

C21 Notice of transfer of a case for reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C21

Effective date: 20220531

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20220614

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20220707

R150 Certificate of patent or registration of utility model

Ref document number: 7103948

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250