DK2778233T3 - Fremgangsmåde til måling af ethanolaminphosphat - Google Patents

Fremgangsmåde til måling af ethanolaminphosphat Download PDF

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DK2778233T3
DK2778233T3 DK12848737.8T DK12848737T DK2778233T3 DK 2778233 T3 DK2778233 T3 DK 2778233T3 DK 12848737 T DK12848737 T DK 12848737T DK 2778233 T3 DK2778233 T3 DK 2778233T3
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DK12848737.8T
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Takushi Ohga
Yoshiaki Ohashi
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Human Metabolome Tech Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/52—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving transaminase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
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  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Claims (18)

1. Fremgangsmåde til måling af ethanolaminphosphat, omfattende: et første trin med tilsætning af et enzym, som kan katalysere en reaktion, der danner acetaldehyd ud fra ethanolaminphosphat, til en prøve og udføre en første enzymatisk reaktion til dannelse af acetaldehyd, phosphorsyre og ammoniak; og et andet trin med kvantificering af mindst en af det/den resulterende acetaldehyd, phosphorsyre og ammoniak til bestemmelse af en mængde af ethanolaminphosphat i måleprøven.
2. Målefremgangsmåde ifølge krav 1, hvor det andet trin omfatter: et undertrin med tilsætning til måleprøven efter den første enzymatiske reaktion, af acetaldehyd-dehydrogenase (ALDH) og oxideret nicotinamid-adenindinukleotid (NAD+) og udførelse af en anden enzymatisk reaktion til dannelse af reduceret nicotinamid-adenindinukleotid (NADH); og et andet undertrin med spektrokemisk kvantificering af det reducerede nicotinamid-adenindinukleotid (NADH) til kvantificering af acetaldehyd i måleprøven efter den første enzymatiske reaktion.
3. Målefremgangsmåde ifølge krav 1 eller 2, hvor der i første trin yderligere tilsættes et coenzym til måleprøven.
4. Målefremgangsmåde ifølge krav 3, hvor coenzymet er pyridoxalphosphat.
5. Målefremgangsmåde ifølge et hvilket som helst af kravene 1-4, hvor enzymet til anvendelse i det første trin er et protein, som har en aminosyrese-kvensregion med et GabT-domæne bestående af følgende aminosyrese-kvens: GXXXBADEBQXGFZRXG, hvor X betyder en aminosyre, B betegner en forgrenet aminosyre udvalgt fra gruppen bestående af I, L og V, og Z betyder G eller A.
6. Målefremgangsmåde ifølge et hvilket som helst af kravene 1-4, hvor enzymet til anvendelse i det første trin er: (1) et protein bestående af en aminosyresekvens betegnet med SEQ ID NO:1 eller 2, (2) et protein bestående af en aminosyresekvens, hvor, i aminosyresekven-sen betegnet med SEQ ID NO:1 eller 2, en aminosyresekvens er blevet dele-teret, substitueret eller tilsat, eller (3) et protein bestående af en aminosyresekvens med mindst 90 % homologi med aminosyresekvensen betegnet med SEQ ID NO:1 eller 2.
7. Program til muliggørelse af, at en computer udfører fremgangsmåden til måling af ethanolaminphosphat ifølge et hvilket som helst af kravene 1-6.
8. Program ifølge krav 7, yderligere omfattende et trin med bestemmelse, baseret på en måleværdi af ethanolaminphosphat i måleprøven, af hvorvidt et individ, som har tilvejebragt måleprøven, er en depressiv patient, og et andet trin med udlæsning af et således opnået bestemmelsesresultat.
9. Registreringsmedium, der er læsbart af computeren med programmet ifølge krav 7 eller 8 lagret deri.
10. Målesystem med programmet ifølge krav 7 eller 8 inkorporeret deri.
11. Anvendelse af et reagens omfattende et enzym, som kan katalysere en reaktion, der danner acetaldehyd ud fra ethanolaminphosphat som et in vitro-ethanolaminphosphat-målereagens.
12. Anvendelse af reagenset ifølge krav 11, hvor reagenset yderligere omfatter et coenzym for enzymet.
13. Anvendelse af reagentet ifølge krav 12, hvor coenzymet er pyridoxalphosphat.
14. Anvendelse af reagenset ifølge et hvilket som helst af kravene 11-13, hvor enzymet er et protein, som har en aminosyresekvensregion med et GabT-domæne bestående af følgende aminosyresekvens: GXXXBAD EBQXG FZRXG, hvor X betyder en aminosyre, B betegner en forgrenet aminosyre udvalgt fra gruppen bestående af I, L og V, og Z betyder G eller A.
15. Middel omfattende ethanolaminphosphat-målereagenset ifølge et hvilket som helst af kravene 11-14 til anvendelse som et middel til diagnosticering af depression.
16. Anvendelse af et kit omfattende en beholder med et enzym, som kan katalysere en reaktion, der danner acetaldehyd ud fra ethanolaminphosphat, indeholdt separat deri til måling af ethanolaminphosphat in vitro.
17. Anvendelse ifølge krav 16, hvor kittet yderligere omfatter en anden beholder med et coenzym til enzymet, hvilket coenzym er indeholdt separat i den anden beholder.
18. Anvendelse ifølge krav 16 eller 17, hvor enzymet er et protein med en aminosyresekvensregion med et GabT-domæne bestående af følgende ami-nosyresekvens: GXXXBADEBQXGFZRXG, hvor X betyder en aminosyre, B betegner en forgrenet aminosyre udvalgt fra gruppen bestående af I, L og V, og Z betyder G eller A.
DK12848737.8T 2011-11-10 2012-11-06 Fremgangsmåde til måling af ethanolaminphosphat DK2778233T3 (da)

Applications Claiming Priority (2)

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JP2011246881 2011-11-10
PCT/JP2012/078749 WO2013069645A1 (ja) 2011-11-10 2012-11-06 エタノールアミンリン酸の測定方法

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DK2778233T3 true DK2778233T3 (da) 2017-03-20

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EP (1) EP2778233B1 (da)
JP (2) JP5688163B2 (da)
CN (2) CN106399458A (da)
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WO (1) WO2013069645A1 (da)

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CN106716129B (zh) * 2014-09-26 2019-04-19 福满代谢组技术有限公司 对抑郁症治疗药物的选项进行预测的方法
WO2016122446A1 (en) 2015-01-26 2016-08-04 Schlumberger Canada Limited Electrically conductive fiber optic slickline for coiled tubing operations
US10049789B2 (en) 2016-06-09 2018-08-14 Schlumberger Technology Corporation Compression and stretch resistant components and cables for oilfield applications
JP6467110B2 (ja) * 2016-09-30 2019-02-06 ヒューマン・メタボローム・テクノロジーズ株式会社 エタノールアミンリン酸の測定方法
WO2018061194A1 (ja) * 2016-09-30 2018-04-05 ヒューマン・メタボローム・テクノロジーズ株式会社 エタノールアミンリン酸の測定方法
JP7283469B2 (ja) * 2018-03-26 2023-05-30 東洋紡株式会社 エタノールアミンリン酸リアーゼ組成物
EP3821815A4 (en) 2018-07-13 2021-12-29 Life Science Institute, Inc. Mental/nervous system disorder estimation system, estimation program, and estimation method
EP3896169A4 (en) * 2018-12-13 2022-08-31 Kikkoman Corporation METHOD FOR QUANTIFICATION OF ETHANOLAMINE PHOSPHATE, OXIDE REDUCTASE FOR QUANTIFICATION, QUANTIFICATION COMPOSITION, QUANTIFICATION KIT AND SENSOR FOR QUANTIFICATION
JPWO2021256503A1 (da) 2020-06-17 2021-12-23
US20230137741A1 (en) 2021-11-01 2023-05-04 Matterworks Inc Methods and compositions for analyte quantification
US12100484B2 (en) 2021-11-01 2024-09-24 Matterworks Inc Methods and compositions for analyte quantification

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US9631224B2 (en) 2017-04-25
CN103797128A (zh) 2014-05-14
JP2014236749A (ja) 2014-12-18
CN103797128B (zh) 2016-09-07
EP2778233A4 (en) 2015-06-03
EP2778233B1 (en) 2017-01-11
WO2013069645A1 (ja) 2013-05-16
US20170198334A1 (en) 2017-07-13
US20160208310A1 (en) 2016-07-21
JP5688163B2 (ja) 2015-03-25
CN106399458A (zh) 2017-02-15
EP2778233A1 (en) 2014-09-17
JPWO2013069645A1 (ja) 2015-04-02
US10294509B2 (en) 2019-05-21

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