DK2748315T3 - Glucoamylasevarianter og polynukleotider, som koder for dem - Google Patents

Glucoamylasevarianter og polynukleotider, som koder for dem Download PDF

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Publication number
DK2748315T3
DK2748315T3 DK12761850.2T DK12761850T DK2748315T3 DK 2748315 T3 DK2748315 T3 DK 2748315T3 DK 12761850 T DK12761850 T DK 12761850T DK 2748315 T3 DK2748315 T3 DK 2748315T3
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glucoamylase
variant
polypeptide
seq
alpha
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DK12761850.2T
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English (en)
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Tomoko Matsui
Suzanne Clark
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Novozymes North America Inc
Novozymes As
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00—Other raw materials for the preparation of beer
    • C12C5/004—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C13—SUGAR INDUSTRY
    • C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
    • C13K1/00—Glucose; Glucose-containing syrups
    • C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Emergency Medicine (AREA)
  • Food Science & Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (18)

1. Glucoamylasevariant, som omfatter en substitution i mindst en position svarende til position 79 i det modne polypeptid ifølge SEQ ID NO: 2, hvor varianten har glucoamylaseaktivitet, og hvor varianten er valgt fra gruppen bestående af: a) et polypeptid, som udviser mindst 85% sekvensidentitet med det modne polypeptid ifølge SEQ ID NO: 2; b) et polypeptid, som kodes for af et polynukleotid, som udviser mindst 85% identitet med det modne polypeptids kodningssekvens ifølge SEQ ID NO: 1; og (c) et fragment af det modne polypeptid ifølge SEQ ID NO: 2, som har glucoamylaseaktivitet,
2. Variant ifølge krav 1, hvor antallet af substitutioner er 1 -20, f.eks. 1 -10 og 1 -5, såsom 1, 2. 3, 4, 5, 6, 7, 8, 9 eller 10 substitutioner.
3. Variant ifølge et hvilket som helst af de foregående krav, hvor varianten omfatter en substitution valgt blandt K79V, K79A, K79G, K79I, K79L, K79S, K79T.
4. Variant ifølge et hvilket som helst af de foregående krav, hvor varianten omfatter substitution K79V.
5. Variant ifølge et hvilket som helst af kravene 1-4, som har en forbedret egenskab sammenlignet med stamglucoamylasen, hvor den forbedrede egenskab er reduceret følsomhed overfor proteasenedbrydning.
6. Katalytisk glucoamylase-variantdomæne, som omfatter en substitution i mindst en position svarende til position 79 i det modne polypeptid ifølge SEQ ID NO: 2, og hvor varianten er valgt fra gruppen bestående af: (a) et katalytisk domæne, som udviser mindst 85% sekvensidentitet med aminosyrerne 30 til 494 ifølge SEQ ID NO: 2; (b) et katalytisk domæne, som kodes for af et polynukleotid, der udviser mindst 85% sekvensidentitet med (i) nukleotiderne 88 til 1482 ifølge SEQ ID NO: 1; og hvor det katalytiske domæne har glucoamylaseaktivitet.
7. Sammensætning, som omfatter polypeptidet ifølge et hvilket som helst af kravene 1-6.
8. Anvendelse af et polypeptid ifølge et hvilket som helst af kravene 1-6 til fremstilling af sirup og/eller et fermenteringsprodukt.
9. Anvendelse af et polypeptid ifølge et hvilket som helst af kravene 1-6 til brygning.
10. Fremgangsmåde til fremstilling af et fermenteringsprodukt ud fra stivelsesholdigt materiale, hvilken fremgangsmåde omfatter følgende trin: (a) at gøre stivelsesholdigt materiale flydende i nærværelse af en alfa-amylase; (b) at forsukre materialet, som er gjort flydende; og (c) at fermentere med en fermenterende organisme; hvor trin (a) og/eller trin (b) udføres ved anvendelse af mindst en glucoamylase ifølge et hvilket som helst af kravene 1-6.
11. Fremgangsmåde til fremstilling af et fermenteringsprodukt ud fra stivelsesholdigt materiale, hvilken fremgangsmåde omfatter følgende trin: (a) at forsukre stivelsesholdigt materiale ved en temperatur, som er lavere end den laveste gelatineringstemperatur for det stivelsesholdige materiale; og (b) at fermentere med en fermenterende organisme, hvor trin (a) udføres ved anvendelse af mindst en glucoamylase ifølge et hvilket som helst af kravene 1-6.
12. Isoleret polynukleotid, som koder for polypeptidet ifølge et hvilket som helst af kravene 1-6.
13. Nukleinsyrekonstruktion eller ekspressionsvektor, som omfatter polynukleotidet ifølge krav 12, hvilket polynukleotid er operativt forbundet til en eller flere kontrolsekvenser, der styrer fremstillingen af polypeptidet i en ekspressionsvært.
14. Rekombinant værtscelle, som omfatter polynukleotidet ifølge krav 12, hvilket polynukleotid er operativt forbundet til en eller flere kontrolsekvenser, der styrer fremstillingen af polypeptidet.
15. Fremgangsmåde til fremstilling af et polypeptid ifølge et hvilket som helst af kravene 1 -6, hvilken fremgangsmåde omfatter: (a) at dyrke værtscellen ifølge krav 14 under betingelser, som er befordrende for fremstilling af polypeptidet; og (b) at indvinde polypeptidet.
16. Nukleinsyrekonstruktion, som omfatter polynukleotidet ifølge krav 12.
17. Ekspressionsvektor, som omfatter polynukleotidet ifølge krav 12.
18. Værtscelle, som omfatter polynukleotidet ifølge krav 12.
DK12761850.2T 2011-09-06 2012-09-05 Glucoamylasevarianter og polynukleotider, som koder for dem DK2748315T3 (da)

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US201161531189P 2011-09-06 2011-09-06
US201161566046P 2011-12-02 2011-12-02
PCT/US2012/053779 WO2013036526A1 (en) 2011-09-06 2012-09-05 Glucoamylase variants and polynucleotides encoding same

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EP (1) EP2748315B1 (da)
CN (1) CN103930543B (da)
CA (1) CA2842248C (da)
DK (1) DK2748315T3 (da)
EA (1) EA201490568A1 (da)
ES (1) ES2656556T3 (da)
IN (1) IN2014CN02469A (da)
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WO (1) WO2013036526A1 (da)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9951323B2 (en) 2013-07-17 2018-04-24 Novozymes A/S Pullulanase chimeras and polynucleotides encoding same
EP3712274A1 (en) 2013-09-11 2020-09-23 Novozymes A/S Processes for producing fermentation products
HUE056192T2 (hu) 2014-01-22 2022-01-28 Novozymes As Pullulanázvariánsok és azokat kódoló polinukleotidok
BR112016018075B1 (pt) 2014-02-07 2022-01-18 Novozymes A/S Composição compreendendo uma alfa-amilase, uma pululanase e uma enzima glicoamilase e método de fabricação de xarope de glicose a partir de amido liquefeito
US10370682B2 (en) 2014-06-25 2019-08-06 Novozymes A/S Xylanase variants and polynucleotides encoding same
US10584324B2 (en) 2014-10-23 2020-03-10 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
EP3227452A1 (en) 2014-12-01 2017-10-11 Novozymes A/S Improved production of glucose syrups
CN108350442A (zh) 2015-06-18 2018-07-31 诺维信公司 具有海藻糖酶活性的多肽及其在产生发酵产物的方法中的用途
EP3394274A1 (en) 2015-12-22 2018-10-31 Novozymes A/S Processes for producing fermentation products
WO2018098381A1 (en) 2016-11-23 2018-05-31 Novozymes A/S Improved yeast for ethanol production
CN110603322A (zh) * 2017-03-07 2019-12-20 丹尼斯科美国公司 热稳定的葡糖淀粉酶及其使用方法
WO2018222990A1 (en) 2017-06-02 2018-12-06 Novozymes A/S Improved yeast for ethanol production
CN110997701A (zh) 2017-06-28 2020-04-10 诺维信公司 具有海藻糖酶活性的多肽以及编码其的多核苷酸
US11220679B2 (en) 2017-08-08 2022-01-11 Novozymes A/S Polypeptides having trehalase activity
US20200263207A1 (en) 2017-09-15 2020-08-20 Novozymes A/S Enzyme blends and processes for improving the nutritional quality of animal feed
WO2019083831A1 (en) 2017-10-23 2019-05-02 Novozymes A/S METHODS FOR LACTIC ACID REDUCTION IN A BIOCARBURIZER FERMENTATION SYSTEM
AU2019213033A1 (en) 2018-01-29 2020-09-17 Microbiogen Pty. Ltd. Microorganisms with improved nitrogen utilization for ethanol production
CA3107124A1 (en) 2018-02-15 2019-08-22 Novozymes A/S Improved yeast for ethanol production
MX2020012754A (es) 2018-05-31 2021-02-26 Novozymes As Procesos para mejorar el crecimiento y la productividad de la levadura.
WO2020014407A1 (en) 2018-07-11 2020-01-16 Novozymes A/S Processes for producing fermentation products
BR112021001282A2 (pt) 2018-07-25 2021-04-27 Novozymes A/S levedura expressando enzimas para produção de etanol
WO2020076697A1 (en) 2018-10-08 2020-04-16 Novozymes A/S Enzyme-expressing yeast for ethanol production
BR112021014873A2 (pt) 2019-01-31 2021-10-05 Novozymes A/S Polipeptídeo, combinação de enzimas, polinucleotídeo, construto de ácido nucleico ou vetor de expressão recombinante, célula hospedeira recombinante, método de produção de um polipeptídeo, e, processo de produção de um produto de fermentação
WO2021021458A1 (en) 2019-07-26 2021-02-04 Novozymes A/S Microorganisms with improved nitrogen transport for ethanol production
BR112022002203A2 (pt) 2019-08-05 2022-09-06 Novozymes As Misturas de enzimas e processos para produção de um ingrediente alimentar com alto teor de proteína a partir de um subproduto de vinhaça completa
EP4010469A1 (en) 2019-08-06 2022-06-15 Novozymes A/S Fusion proteins for improved enzyme expression
BR112022011271A2 (pt) 2019-12-10 2022-09-06 Novozymes As Célula hospedeira recombinante, composição, métodos para produzir um derivado de uma célula e um produto de fermentação, e, uso de uma célula hospedeira recombinante
BR112022011634A2 (pt) 2019-12-16 2022-08-30 Novozymes As Processos para produção de um produto de fermentação, para diminuição da quantidade de amido residual presente em um liquefato e para aumento da quantidade de oligossacarídeos de cadeia curta presente em um liquefato
WO2022261003A1 (en) 2021-06-07 2022-12-15 Novozymes A/S Engineered microorganism for improved ethanol fermentation
WO2024137250A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
WO2024137704A2 (en) 2022-12-19 2024-06-27 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast
WO2024137246A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same
WO2024137252A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Process for reducing syrup viscosity in the backend of a process for producing a fermentation product
WO2024137248A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK135983D0 (da) 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
JP2703598B2 (ja) 1987-09-04 1998-01-26 ノボ―ノルディスク アクティーゼルスカブ アスペルギルス菌中でのタンパク質生産物の生産方法およびアスペルギルス菌中で使用するためのプロモーター
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
DK81293D0 (da) 1993-07-06 1993-07-06 Novo Nordisk As Enzym
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
MX9606013A (es) 1994-06-03 1997-12-31 Novo Nordisk Biotech Inc Lacasas purificadas de myceliophthora y acidos nucleicos que codifican para las mismas.
EP0777737B1 (en) 1994-06-30 2005-05-04 Novozymes Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US6093562A (en) 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
ES2329528T3 (es) 1995-02-03 2009-11-26 Novozymes A/S Metodo para diseñar mutantes alfa-amilasa con propiedades determinadas.
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
WO1997041213A1 (en) 1996-04-30 1997-11-06 Novo Nordisk A/S α-AMYLASE MUTANTS
JP4358431B2 (ja) 1997-10-13 2009-11-04 ノボザイムス アクティーゼルスカブ α−アミラーゼ変異体
EP1025212B1 (en) 1997-10-14 2007-09-26 Darwin Molecular Corporation Thymidine kinase mutants and fusion proteins having thymidine kinase and guanylate kinase activities
AR014402A1 (es) 1997-11-26 2001-02-28 Novozymes As Glucoamilasa termoestable proceso para convertir almidon o almidon parcialmente hidrolizado en un jarabe que contiene dextrosa
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
AU757935B2 (en) 1998-02-27 2003-03-13 Novozymes A/S Maltogenic alpha-amylase variants
ATE332968T1 (de) 1998-10-26 2006-08-15 Novozymes As Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen
EP2278016B1 (en) 1999-03-22 2012-09-26 Novozymes Inc. Promoter sequences derived from Fusarium Venenatum and uses thereof
DE60034558T2 (de) 1999-03-30 2007-12-27 Novozymes A/S Alpha-amylase-varianten
US7884066B2 (en) * 1999-10-05 2011-02-08 The Regents Of The University Of California NELL-1 enhanced bone mineralization
CA2416967C (en) 2000-08-01 2014-02-18 Novozymes A/S Alpha-amylase mutants with altered properties
AU2002319696B2 (en) 2001-07-27 2007-11-01 The Government Of The United States Of America As Represented By The Secretary Of Health And Human Services Systems for in vivo site-directed mutagenesis using oligonucleotides
WO2003048353A1 (en) 2001-12-07 2003-06-12 Novozymes A/S Polypeptides having protease activity and nucleic acids encoding same
US7785601B2 (en) * 2002-12-31 2010-08-31 Sygnis Bioscience Gmbh & Co. Kg Methods of treating neurological conditions with hematopoietic growth factors
ES2245621T3 (es) 2003-03-10 2010-05-19 Novozymes A/S Procesos para un producto alcoholico.
ATE510925T1 (de) 2003-06-25 2011-06-15 Novozymes As Stärkehydrolyseverfahren
MXPA06000212A (es) 2003-06-25 2006-03-21 Novozymes As Enzimas para procesar almidon.
DK1682655T3 (da) 2003-10-30 2010-01-11 Novozymes As Carbohydratbindende moduler fra en ny familie
ES2621921T3 (es) 2004-12-22 2017-07-05 Novozymes A/S Enzimas para tratamiento de almidón
WO2006091194A1 (en) * 2005-02-23 2006-08-31 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
ATE448319T1 (de) 2006-06-15 2009-11-15 Novozymes As Verfahren zur herstellung eines stärkehydrolysats
MX355362B (es) 2009-12-11 2018-04-17 Novozymes As Variantes de proteasa.
CN102918149A (zh) 2010-01-04 2013-02-06 诺维信公司 α-淀粉酶
ES2565060T3 (es) 2010-04-14 2016-03-31 Novozymes A/S Polipéptidos que tienen actividad de glucoamilasa y polinucleótidos que codifican los mismos
DK2654567T3 (da) * 2010-12-22 2018-06-25 Novozymes North America Inc Fremgangsmåde til fremstilling af fermenteringsprodukter ud fra stivelsesholdige materialer

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EA201490568A1 (ru) 2014-06-30
US9534239B2 (en) 2017-01-03
WO2013036526A1 (en) 2013-03-14
CN103930543B (zh) 2020-11-27
EP2748315B1 (en) 2017-11-15
CA2842248C (en) 2021-02-16
EP2748315A1 (en) 2014-07-02
MX351154B (es) 2017-10-04
US20140342422A1 (en) 2014-11-20
CN103930543A (zh) 2014-07-16
CA2842248A1 (en) 2013-03-14
MX2014002415A (es) 2014-05-28
ES2656556T3 (es) 2018-02-27

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