DK2992092T3 - Glucoamylasevarianter og polynukleotider, som koder for dem - Google Patents

Glucoamylasevarianter og polynukleotider, som koder for dem Download PDF

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Publication number
DK2992092T3
DK2992092T3 DK14723388.6T DK14723388T DK2992092T3 DK 2992092 T3 DK2992092 T3 DK 2992092T3 DK 14723388 T DK14723388 T DK 14723388T DK 2992092 T3 DK2992092 T3 DK 2992092T3
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Denmark
Prior art keywords
glucoamylase
variant
alpha
ser
ala
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DK14723388.6T
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English (en)
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Noriko Tsutsumi
Keiichi Ayabe
Siik Kishishita
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Novozymes As
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (10)

1. Glucoamylasevariant, som omfatter en substitution i en eller fiere positioner svarende til position 95, 59, 119 og 121 i polypeptidet ifolge SEQ ID NO: 3, hvor varianten har glucoamylaseaktivitet, og hvor varianten udviser mindst 90%, mindst 95%, mindst 96%, mindst 97%, mindst 98% eller mindst 99%, men mindre end 100% sekvensidentitet med polypeptidet ifolge SEQ ID NO: 3, hvilken variant omfatter en eller fiere substitutioner valgt fra gruppen bestaende af 59A, 95P, 119W og 121P, og hvor varianten omfatter mindst én af folgende substitutioner eller kombinationer af substitutioner: V59A; S95P; A121P; T119W; S95P + A121P; V59A + S95P; S95P + T119W; V59A + S95P + A121P; eller S95P + T119W + A121P; og hvor varianterne harforoget termostabilitet i sammenligning med stamglucoamylasen ifolge SEQ ID NO: 3.
2. Sammensaetning, som omfatter polypeptidet ifolge krav 1.
3. Sammensaetning ifolge krav 2, som omfatter en alfa-amylase.
4. Anvendelse af et polypeptid ifolge krav 1 til fremstilling af sirup og/eller et fermenteringsprodukt.
5. Fremgangsmade til fremstilling af et fermenteringsprodukt ud fra stivelsesholdigt materiale, hvilken fremgangsmade omfatter folgende trin: (a) at gore stivelsesholdigt materiale flydende i naervaerelse af en alfa-amylase; (b) at forsukre materialet, som er gjort flydende; og (c) at fermentere med en fermenterende organisme; hvor trin (a) og/eller trin (b) udfores ved anvendelse af mindst en glucoamylasevariant ifolge krav 1.
6. Fremgangsmade til fremstilling af et sirupprodukt ud fra stivelsesholdigt materiale, hvilken fremgangsmade omfatter folgende trin: (a) at g0re stivelsesholdigt materiale flydende i naervaerelse at en alfa-amylase; (b) at forsukre materialet, som er gjort flydende, i naervaerelse af en glucoamylasevariant ifolge krav 1.
7. Isoleret polynukleotid, som koder for varianten ifolge krav 1.
8. Nukleinsyrekonstruktion, som omfatter polynukleotidet ifolge krav 7.
9. Ekspressionsvektor, som omfatter polynukleotidet ifolge krav 7.
10. Vaertscelle, som omfatter polynukleotidet ifolge krav 7.
DK14723388.6T 2013-04-30 2014-04-29 Glucoamylasevarianter og polynukleotider, som koder for dem DK2992092T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13165995 2013-04-30
PCT/EP2014/058692 WO2014177546A2 (en) 2013-04-30 2014-04-29 Glucoamylase variants and polynucleotides encoding same

Publications (1)

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DK2992092T3 true DK2992092T3 (da) 2018-06-25

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US (1) US10550374B2 (da)
EP (2) EP3372680B1 (da)
CN (1) CN105164253A (da)
BR (1) BR112015026972B1 (da)
CA (1) CA2904022C (da)
DK (2) DK3372680T3 (da)
ES (2) ES2835313T3 (da)
MX (2) MX368655B (da)
WO (1) WO2014177546A2 (da)

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Also Published As

Publication number Publication date
DK3372680T3 (da) 2021-01-11
WO2014177546A2 (en) 2014-11-06
CA2904022C (en) 2022-04-19
EP3372680B1 (en) 2020-11-11
CN105164253A (zh) 2015-12-16
BR112015026972B1 (pt) 2023-05-02
EP2992092B1 (en) 2018-04-04
MX2015014537A (es) 2016-02-05
EP3372680A1 (en) 2018-09-12
ES2835313T3 (es) 2021-06-22
WO2014177546A3 (en) 2014-12-24
US20160152960A1 (en) 2016-06-02
US10550374B2 (en) 2020-02-04
BR112015026972A2 (da) 2017-09-05
CA2904022A1 (en) 2014-11-06
ES2674701T3 (es) 2018-07-03
MX368655B (es) 2019-10-10
EP2992092A2 (en) 2016-03-09
MX2019005158A (es) 2019-08-05

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