DK2609201T3 - Mutante delta-9-elongaser og anvendelse heraf til fremstilling af polyumættede fedtsyrer - Google Patents

Mutante delta-9-elongaser og anvendelse heraf til fremstilling af polyumættede fedtsyrer Download PDF

Info

Publication number
DK2609201T3
DK2609201T3 DK11750066.0T DK11750066T DK2609201T3 DK 2609201 T3 DK2609201 T3 DK 2609201T3 DK 11750066 T DK11750066 T DK 11750066T DK 2609201 T3 DK2609201 T3 DK 2609201T3
Authority
DK
Denmark
Prior art keywords
seq
leu
delta
phe
tyr
Prior art date
Application number
DK11750066.0T
Other languages
English (en)
Inventor
Michael W Bostick
Hongxian He
Yougen Li
Qun Zhu
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Application granted granted Critical
Publication of DK2609201T3 publication Critical patent/DK2609201T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01016—Acetyl-CoA C-acyltransferase (2.3.1.16)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fats And Perfumes (AREA)

Claims (15)

  1. MUTANTE DELTA-9-ELONGASER OG ANVENDELSE HERAF TIL FREMSTILLING AF POLYUMÆTTEDE FEDTSYRER
    1. Isoleret polynukleotid, der omfatter: (a) en nukleotidsekvens, der koder for et mutant polypeptid, der har delta-9-elongaseaktivitet, og som har en aminosyresekvens som vist i SEQ ID NO: 22, hvor SEQ ID NO: 22 adskiller sig fra SEQ ID NO: 10 ved mindst én aminosyremutation, hvilken eller hvilke mutationer er udvalgt fra gruppen bestående af: i) en L35G-mutation; ii) en L35F-mutation; iii) en L35M-mutation; iv) en L35G-mutation og mindst én anden mutation, der er udvalgt fra gruppen bestående af: S9A, S9D, S9G, S9I, S9K, S9Q, Q12K, A21D, A21T, A21V, V32F, Y84C, L108G, G127L, W132T, M143N, M143W, L161T, L161Y, W168G, I179M, I179R, C236N, Q244N, A254W og A254Y; v) L35G-, A21V-, L108G- og I179R-mutationer; vi) L35G-, W132T- og I179R-mutationer; vii) L35G-, S9D-, Y84C- og I179R-mutationer; viii) L35G-, Y84C-, I179R- og Q244N-mutationer; ix) L35G-, A21V-, W132T-, I179R- og Q244N-mutationer; x) L35M- og Q107E-mutationer; xi) K58R-og I257T-mutationer; xii) en D98G-mutation og xiii) L130M- samt V243A-mutationer; eller (b) et komplement af nukleotidsekvensen fra del (a), hvor komplementet og nukleotidsekvensen består af det samme antal nukleotider og er 100 % komplimentære.
  2. 2. Isoleret polynukleotid ifølge krav 1, hvor nukleotidsekvensen koder for et mutant polypeptid, der er udvalgt fra gruppen bestående af SEQ ID NO: 59, SEQ ID NO: 29, SEQ ID NO: 32, SEQ ID NO: 35, SEQ ID NO: 38, SEQ ID NO: 41, SEQ ID NO: 62, SEQ ID NO: 87, SEQ ID NO: 101, SEQ ID NO: 104, SEQ ID NO: 107 og SEQ ID NO: 110.
  3. 3. Isoleret polynukleotid ifølge krav 2, hvor nukleotidsekvensen koder for et mutant polypeptid som vist i SEQ ID NO: 59.
  4. 4. Mutant polypeptid, der har delta-9-elongaseaktivitet, der er kodet for af det isolerede polynukleotid ifølge krav 1, hvor proteinsekvensen fortrinsvis er udvalgt fra gruppen bestående af: SEQ ID NO; 59, SEQ ID NO: 29, SEQ ID NO: 32, SEQ ID NO: 35, SEQ ID NO: 38, SEQ ID NO: 41, SEQ ID NO: 62, SEQ ID NO: 87, SEQ ID NO: 101, SEQ ID NO: 104, SEQ ID NO: 107 og SEQ ID NO: 110.
  5. 5. Mutant polypeptid ifølge krav 4, hvor den procentvise substratomdannelse af linolsyre til eicosadiensyre af delta-9-elongasen er mindst 110 % sammenlignet med den procentvise substratomdannelse af linolsyre til eicosadiensyre af polypeptidet som vist i SEQ ID NO: 10, hvor mere fortrinsvis den procentvise substratomdannelse af linolsyre til eicosadiensyre er mindst 120 % sammenlignet med den procentvise substratomdannelse af linolsyre til eicosadiensyre af polypeptidet som visti SEQ ID NO: 10.
  6. 6. Rekombinant konstrukt, der omfatter det isolerede polynukleotid ifølge krav 1, og som er operativt forbundet med mindst én regulatorisk sekvens.
  7. 7. Celle transformeret med det rekombinante konstrukt ifølge krav 6.
  8. 8. Transformeret celle ifølge krav 7, hvor cellen er udvalgt fra gruppen bestående af: planter, bakterier, gær, alger, euglenoider, stramenopiler, oomyceter og svampe.
  9. 9. Transformeret celle ifølge krav 8, hvor cellen er en olieholdig gær, der producerer mindst omkring 25 % af sin tørcellevægt som olie.
  10. 10. Transformeret celle ifølge krav 9, hvor den olieholdige gær endvidere omfatter mindst ét rekombinant DNA-konstrukt, der omfatter et isoleret polynukleotid, der er operativt bundet til mindst én regulatorisk sekvens, hvilket rekombinant DNA-konstrukt koder for et polypeptid, der er udvalgt fra gruppen bestående af: delta-4-desaturase, delta-5-desaturase, delta-8-desaturase, delta-6-desaturase, delta-9-desaturase, delta-12-desaturase, delta-15-desaturase, delta-17-desaturase, Ci4/]6-elongase, Ci6/is-elongase, Cis/20-elongase og C20/22-elongase.
  11. 11. Transformeret celle ifølge krav 10, hvor olie, der er produceret af den olieholdige gær, omfatter en langkædet polyumættet fedtsyre, der er udvalgt fra gruppen bestående af: arachidonsyre, eicosadiensyre, eicosapentaensyre, eicosatetraensyre, eicosatriensyre, dihomo-y-linolensyre, docosatetraensyre, docosapentaensyre og docosahexaensyre.
  12. 12. Transformeret celle ifølge et hvilket som helst af kravene 8 til 11, hvor gæren er en olieholdig gær, der er udvalgt fra gruppen bestående af: Yarrowia, Candida, Rhodotorula, Rhodosporidium, Cryptococcus, Trichosporon og Lipomyces.
  13. 13. Transformeret celle ifølge krav 12, hvor cellen er en Yarrowia lipolytica.
  14. 14. Fremgangsmåde til fremstilling af en polyumættet fedtsyre, hvilken fremgangsmåde omfatter: a) tilvejebringelse af en olieholdig gær, der omfatter: i) et rekombinant konstrukt, der er operativt bundet til mindst én regulatorisk sekvens, hvor det rekombinante konstrukt omfatter et isoleret polynukleotid, der koder for et mutant polypeptid, der har delta-9-elongaseaktivitet og har en amino syre sekvens som vist i SEQ ID NO: 22, hvor SEQ ID NO: 22 adskiller sig fra SEQ ID NO: 10 ved mindst én amino syremutation, hvilke(n) mutation(er) er udvalgt fra gruppen bestående af: (a) en L35G-mutation; (b) en L35F-mutation; (c) en L35M-mutation; (d) en L35G-mutation og mindst én anden mutation, der er udvalgt fra gruppen bestående af: S9A, S9D, S9G, S9I, S9K, S9Q, Q12K, A21D, A21T, A21V, V32F, Y84C, L108G, G127L, W132T, M143N, M143W, L161T, L161Y, W168G, I179M, I179R, C236N, Q244N, A254W og A254Y; (e) L35G, A21V, L108G og I179R-mutationer; (Γ) L35G-, W132T- og I179R-mutationer; (g) L35G-, S9D-, Y84C- og I179R-mutationer; (h) L35G-, Y84C-, I179R- og Q244N-mutationer; (i) L35G-, A21V-, W132T-, I179R- og Q244N-mutationer; (j) L35M- og Q107E-mutationer; (k) K58R- og I257T-mutationer; (l) en D98G-mutation og (m) L130M- samt V243A-mutationer, og ii) en kilde til substratfedtsyre, der er udvalgt fra gruppen bestående af linolsyre og alpha-linolensyre; b) dyrkning af gæren fra trin (a) under forhold, hvor det rekombinante konstrukt, der koder for det mutante polypeptid med delta-9-elongaseaktivitet, udtrykkes, og substratfedtsyren omdannes til produktfedtsyre, hvor linolsyre omdannes til eicosadiensyre, og alpha-linolensyre omdannes til eicosatriensyre og; c) eventuelt indvinding af produktfedtsyren fra trin (b), hvor produktfedtsyren fortrinsvis igen indvindes i en oprenset olie.
  15. 15. Rekombinant mikrobiel værtscelle, der producerer en olie, der omfatter mindst 22,5 vægtprocent eicosapentaensyre målt som en vægtprocent af tørcellevægten, hvilken rekombinant mikrobiel værtscelle omfatter mindst ét mutant delta-9-elongasepolypeptid, hvor det mutante delta-9-elongasepolypeptid omfatter en aminosyresekvens som vist i SEQ ID NO: 59, hvor værtscellen omfatter et ekspressionskonstrukt, der indeholder en polynukleotidsekvens, der koder for det mutante polypeptid.
DK11750066.0T 2010-08-26 2011-08-26 Mutante delta-9-elongaser og anvendelse heraf til fremstilling af polyumættede fedtsyrer DK2609201T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37724810P 2010-08-26 2010-08-26
PCT/US2011/049361 WO2012027676A1 (en) 2010-08-26 2011-08-26 Mutant delta-9 elongases and their use in making polyunsaturated fatty acids

Publications (1)

Publication Number Publication Date
DK2609201T3 true DK2609201T3 (da) 2016-06-06

Family

ID=44534726

Family Applications (1)

Application Number Title Priority Date Filing Date
DK11750066.0T DK2609201T3 (da) 2010-08-26 2011-08-26 Mutante delta-9-elongaser og anvendelse heraf til fremstilling af polyumættede fedtsyrer

Country Status (11)

Country Link
US (1) US8980589B2 (da)
EP (1) EP2609201B1 (da)
JP (1) JP5963749B2 (da)
KR (1) KR20140008295A (da)
CN (1) CN103080316B (da)
AU (1) AU2011293167B2 (da)
BR (1) BR112013004356A2 (da)
CA (1) CA2808000A1 (da)
CL (1) CL2013000507A1 (da)
DK (1) DK2609201T3 (da)
WO (1) WO2012027676A1 (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8822497B2 (en) * 2007-03-01 2014-09-02 Novartis Ag PIM kinase inhibitors and methods of their use
CN102978177B (zh) * 2012-11-09 2015-06-03 浙江工业大学 冬虫夏草合成代谢二十碳五烯酸的△-5-脱氢酶、基因及应用
US9005940B2 (en) 2012-12-21 2015-04-14 E I Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a Sou2 sorbitol utilization protein to modify lipid production in microbial cells
CN110358692B (zh) * 2018-04-09 2021-07-27 中国科学院青岛生物能源与过程研究所 生产神经酸的重组酵母菌株及其应用
KR102085555B1 (ko) * 2018-08-27 2020-03-06 대한민국(농촌진흥청장) 아라키돈산 생합성을 위한 신규 델타-5 불포화효소 및 이의 용도

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4880741A (en) 1983-10-06 1989-11-14 Pfizer Inc. Process for transformation of Yarrowia lipolytica
US5071764A (en) 1983-10-06 1991-12-10 Pfizer Inc. Process for integrative transformation of yarrowia lipolytica
US5107065A (en) 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
JP2527340B2 (ja) 1986-12-15 1996-08-21 アプライド バイオシステムズ インコーポレイテッド ロ―ダミン染料の５−及び６−スクシニミジルカルボキシレ―ト異性体
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5648564A (en) 1995-12-21 1997-07-15 Kemin Industries, Inc. Process for the formation, isolation and purification of comestible xanthophyll crystals from plants
GB0107510D0 (en) 2001-03-26 2001-05-16 Univ Bristol New elongase gene and a process for the production of -9-polyunsaturated fatty acids
TWI377253B (en) 2001-04-16 2012-11-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US6797303B2 (en) 2001-09-04 2004-09-28 Lycored Natural Products Industries Ltd. Carotenoid extraction process
ATE400650T1 (de) 2002-10-18 2008-07-15 Sloning Biotechnology Gmbh Verfahren zur herstellung von nukleinsäuremolekülen
EP1576166B1 (en) 2002-12-19 2014-10-15 University Of Bristol Novel method for the production of polyunsaturated fatty acids
US8313911B2 (en) 2003-05-07 2012-11-20 E I Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7259255B2 (en) 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
MX347962B (es) 2003-08-01 2017-05-19 Basf Plant Science Gmbh * Metodo para la produccion de acidos grasos poli-insaturados en organismos transgenicos.
CA2542564A1 (en) 2003-11-12 2005-05-26 E.I. Du Pont De Nemours And Company Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oilseed plants and oleaginous yeast
US7504259B2 (en) 2003-11-12 2009-03-17 E. I. Du Pont De Nemours And Company Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
EP4219670A3 (de) 2004-02-27 2023-08-09 BASF Plant Science GmbH Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen pflanzen
CA2568624C (en) 2004-06-25 2014-03-25 E. I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7550286B2 (en) 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
US7879591B2 (en) 2004-11-04 2011-02-01 E.I. Du Pont De Nemours And Company High eicosapentaenoic acid producing strains of Yarrowia lipolytica
BRPI0609040B1 (pt) 2005-03-18 2018-07-31 Microbia, Inc. Fungo recombinante do gênero yarrowia, método de produção de um carotenóide e método de preparo de um aditivo alimentício ou ração contendo um carotenóide
US7470532B2 (en) 2005-10-19 2008-12-30 E.I. Du Pont De Nemours And Company Mortierella alpina C16/18 fatty acid elongase
KR20080071190A (ko) * 2005-11-23 2008-08-01 이 아이 듀폰 디 네모아 앤드 캄파니 델타-９ 일롱가제, 및 다중불포화 지방산 생성에 있어서의이들의 용도
US7465793B2 (en) * 2006-04-20 2008-12-16 E.I. Du Pont De Nemours And Company Synthetic Δ17 desaturase derived from Phytophthora ramourum and its use in making polyunsaturated fatty acids
US7678560B2 (en) 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
US7556949B2 (en) 2006-10-30 2009-07-07 E.I. Du Pont De Nemours And Company Δ17 desaturase and its use in making polyunsaturated fatty acids
US7709239B2 (en) 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
UA103595C2 (uk) 2007-04-03 2013-11-11 Е. І. Дю Пон Де Немур Енд Компані Мультизими і їх застосування в отриманні поліненасичених жирних кислот
US7790156B2 (en) 2007-04-10 2010-09-07 E. I. Du Pont De Nemours And Company Δ-8 desaturases and their use in making polyunsaturated fatty acids
US8119860B2 (en) 2007-04-16 2012-02-21 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
US7943365B2 (en) 2007-05-03 2011-05-17 E.I. Du Pont De Nemours And Company Δ-5 desaturases and their use in making polyunsaturated fatty acids
US20090117253A1 (en) * 2007-10-03 2009-05-07 E. I. Du Pont De Nemours And Company Peroxisome biogenesis factor protein (pex) disruptions for altering polyunsaturated fatty acids and total lipid content in oleaginous eukaryotic organisms
US8168858B2 (en) * 2008-06-20 2012-05-01 E. I. Du Pont De Nemours And Company Delta-9 fatty acid elongase genes and their use in making polyunsaturated fatty acids
BRPI0913714A2 (pt) 2008-09-19 2020-10-13 E. I. Du Pont De Nemours And Company polipeptídeo mutante que possui atividade de a5 dessaturase, molécula de ácido nucleico isolada, célula hospedeira microbiana e métodos de produção de ácido araquidônico e de ácido eicosapentaenoico
JP5620402B2 (ja) 2008-12-18 2014-11-05 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 発酵プロセスにおけるマロン酸副産物生成の低下
US8399226B2 (en) 2009-06-16 2013-03-19 E I Du Pont De Nemours And Company High eicosapentaenoic acid oils from improved optimized strains of Yarrowia lipolytica

Also Published As

Publication number Publication date
WO2012027676A1 (en) 2012-03-01
EP2609201A1 (en) 2013-07-03
AU2011293167B2 (en) 2016-12-08
CN103080316B (zh) 2016-08-31
EP2609201B1 (en) 2016-03-02
AU2011293167A1 (en) 2013-01-24
JP5963749B2 (ja) 2016-08-03
CL2013000507A1 (es) 2014-03-28
JP2013541328A (ja) 2013-11-14
CA2808000A1 (en) 2012-03-01
CN103080316A (zh) 2013-05-01
US8980589B2 (en) 2015-03-17
KR20140008295A (ko) 2014-01-21
BR112013004356A2 (pt) 2017-06-27
US20120226062A1 (en) 2012-09-06

Similar Documents

Publication Publication Date Title
AU2018220469B2 (en) Method and cell line for production of phytocannabinoids and phytocannabinoid analogues in yeast
CN101848996B (zh) △9延伸酶及其在制备多不饱和脂肪酸中的用途
AU2023226754A1 (en) Compositions and methods for modifying genomes
CA2683497C (en) .delta.8 desaturases and their use in making polyunsaturated fatty acids
CN108138121B (zh) 用微生物高水平生产长链二羧酸
DK2087106T3 (da) Muterende delta8-desaturasegener konstrueret ved målrettet mutagenese og anvendelse heraf i fremstillingen af flerumættede fedtsyrer
DK2576605T3 (da) Fremstilling af metabolitter
CN101365788B (zh) Δ-9延伸酶及其在制备多不饱和脂肪酸中的用途
DK2443248T3 (da) FORBEDRING AF LANGKÆDET POLYUMÆTTET OMEGA-3- OG OMEGA-6- FEDTSYREBIOSYNTESE VED EKSPRESSION AF ACYL-CoA-LYSOPHOSPHOLIPID- ACYLTRANSFERASER
DK2140006T3 (da) Delta-5-desaturaser og anvendelse heraf til fremstilling af polyumættede fedtsyrer
KR20140007430A (ko) 에이코사펜타엔산 농축물
KR20190002470A (ko) 표적 핵산의 변형을 위한 개선된 방법
KR20130138760A (ko) 고농도의 에이코사펜타엔산 생성을 위한 재조합 미생물 숙주 세포
DK2609201T3 (da) Mutante delta-9-elongaser og anvendelse heraf til fremstilling af polyumættede fedtsyrer
KR20070085669A (ko) 고농도의 아라키돈산을 생성하는 야로위아 리폴리티카 균주
KR102096604B1 (ko) 신규한 crispr 연관 단백질 및 이의 용도
CN109996874A (zh) 10-甲基硬脂酸的异源性产生
KR20190121030A (ko) 유기산 내성 효모 유래 신규 프로모터 및 이를 이용한 목적유전자의 발현방법
DK2475679T3 (da) Forbedrede, optimerede stammer af yarrowia lipolytica til fremstilling af højkoncentreret eicosapentaensyre
KR20140000711A (ko) 수크로스 이용에 있어서의 야로위아 리포라이티카에서의 사카로마이세스 세레비지애 suc2 유전자의 용도
KR101566371B1 (ko) 구제역 아시아1 혈청형의 표준백신 바이러스의 방어항원을 발현하는 재조합 구제역 바이러스 및 이의 제조 방법
CN101300358A (zh) 真核细胞中变胞藻黄素的生物合成
KR102108190B1 (ko) 자일로스 이용능을 갖는 효모 균주
CN112639104B (en) Novel promoter derived from organic acid-tolerant yeast and method for expressing target gene using the same
KR20230110271A (ko) 식물성 칸나비노이드들의 생산에 사용하기 위한 개선된 활성을 갖는 올리베톨산 시클라아제 변이체들