KR102108190B1 - 자일로스 이용능을 갖는 효모 균주 - Google Patents

자일로스 이용능을 갖는 효모 균주 Download PDF

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KR102108190B1
KR102108190B1 KR1020180117860A KR20180117860A KR102108190B1 KR 102108190 B1 KR102108190 B1 KR 102108190B1 KR 1020180117860 A KR1020180117860 A KR 1020180117860A KR 20180117860 A KR20180117860 A KR 20180117860A KR 102108190 B1 KR102108190 B1 KR 102108190B1
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yali0
gene
mutation
xylulose
strain
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이선미
육상도
엄영순
공경택
김지원
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한국과학기술연구원
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Abstract

본 명세서에는 자일로스를 탄소원으로 이용할 수 있는 효모 균주 및 이를 이용한 지질 생산방법이 개시된다. 상기 효모 균주는 자일로스를 탄소원으로 이용하지 못하는 야생형 효모 균주를 고농도 지질생산이 가능하도록 적응 진화시킨 후, 상기 적응 진화된 균주를 형질 전환하여 자일로스 대사능을 부여함으로써 산환원효소 기반 자일로스 대사경로를 도입하지 않아 보조인자 불균형의 문제가 없이 지질 및 목질계 바이오매스 기반 바이오디젤과 바이오소재를 높은 수율로 생산할 수 있으며, 바이오디젤 및 바이오소재 생산 공정의 경제성 및 지속가능성을 크게 향상시킬 수 있다.

Description

자일로스 이용능을 갖는 효모 균주 {YEAST STRAIN WITH XYLOSE UTILIZING CAPACITY}
본 명세서에는 자일로스를 탄소원으로 이용할 수 있는 효모 균주 및 이를 이용한 지질 생산방법이 개시된다.
점차 증가하는 바이오디젤 수요에 대응하기 위하여, 기존의 식물성 기름 또는 미세조류에 기반한 바이오디젤 생산의 한계를 극복할 수 있는 새로운 바이오디젤 생산기술로서, 지질을 생산하는 효모 균주를 이용하여 바이오매스로부터 바이오디젤의 원료인 지질을 고농도로 생산하여 바이오디젤의 경제성 및 지속가능성을 향상시킬 수 있는 기술이 개발되고 있다.
효모의 일종인 야로위아 리폴리티카(Yarrowia lipolytica)는 대표적인 지질생산 효모 균주로 형질전환 균주의 경우 글루코스 기반 배지에서 배양 시, 세포 건조 중량의 약 90%까지 지질을 생산한다고 보고된 바 있다. 하지만 해당 균주는 목질계 바이오매스의 대표적 가수 분해물인 오탄당(자일로스(xylose))을 이용하지 못하기 때문에 탄소원의 활용이 제한적이다. 따라서 보다 경제적인 바이오디젤 생산을 위해서 자일로스 대사가 가능한 지질 생산 효모 균주 개발이 필요한 실정이다.
효모 균주를 이용한 지질 생산 공정은 바이오매스를 바이오 연료로 쉽게 전환할 수 있는 경제적인 공정이나, 목질계 바이오매스와 같은 비식용계 바이오매스를 원료로 이용할 경우 바이오매스 유래 육탄당(대표적으로 글루코스, 바이오매스 내 최대 50% 함유)만을 이용하고 오탄당(대표적으로 자일로스, 바이오매스 내 최대 25% 함유)을 이용하지 못하여 전환효율이 떨어지는 문제점이 존재한다. 이를 극복할 경우 바이오매스 최대 전환율을 50%에서 75%까지 크게 향상시키는 것이 가능할 것으로 예상되는바, 이에 자일로스 전환 균주 개발이 진행되고 있다.
효모는 내생적으로 자일로스를 사용할 수 있는 산화환원효소 기반의 자일로스 대사경로를 보유하고 있으나 관련 유전자의 발현 정도가 낮아 야생형의 경우 자일로스 대사가 불가능하다. 이러한 효모에 자일로스 이용능을 부여하기 위하여 산화환원효소 기반의 자일로스 대사경로가 도입된 형질전환 균주 개발이 보고된 바 있다. 그러나 아직 자일로스 이용능이 만족할 만한 수준에 도달하지 않았으며, 특히 산화환원효소 기반의 자일로스 대사경로는 보조인자 불균형 문제뿐만이 아니라 지질 생산 과정에서 소모가 많은 보조인자인 NADPH를 소모하기 때문에 지질 생산능이 떨어질 수 있다는 문제점이 존재한다. 따라서 본 발명자들은 보조인자 문제가 없는 자일로스 이성화효소 기반의 자일로스 대사 효모 균주를 개발하여 목질계 바이오매스(가용 탄소원 중 글루스코 및 자일로스 함유량 각각 최대 50%, 25%)로부터 고수율의 지질 생산이 가능한 형질전환 균주를 개발하고자 하였다. 이에 대사공학과 진화공학을 통하여 적응 진화된 효모 균주에 자일로스 이성화효소 기반의 자일로스 대사경로를 도입함으로써 자일로스 이용능이 향상된 효모 형질전환 균주를 개발하였으며, 이를 이용한 지질생산 방법을 제시하고자 한다.
미국 특허등록공보 US 9,096,876 B2
Rodrigo Ledesma Amaro et al., Metabolic engineering of Yarrowia lipolytica to produce chemicals and fuels from xylose, Met. Eng., 2016, 38, 115-124 Gabriel M. Rodriguez et al., Engineering xylose utilization in Yarrowia lipolytica by understanding its cryptic xylose pathway, Biotechnol. Biofuel., 2016, 9:149 Haibo Li et al., Enabling xylose utilization in Yarrowia lipolytica for lipid production, Biotechnol. J., 2016, 11, 1230-1240
일 측면에서, 본 발명의 목적은, YALI0_A15642g, YALI0_A15796g, YALI0_C11165g, YALI0_C16247g, YALI0_D24849g, YALI0_D27016g, YALI0_E14388g1, YALI0_E23969g, YALIO_F04906g 및 YALI0_F17468g로 이루어진 군으로부터 선택된 하나 이상의 유전자에서 하기의 돌연변이를 포함하고, D-자일로스(D-xylose)와 D-자일룰로스(D-xylulose)를 상호 변환하는 효소를 코딩하는 유전자 및 D-자일룰로스(D-xylulose)로부터 D-자일룰로스-5-인산염(D-xylulose-5-phosphate)을 생산하는 효소를 코딩하는 유전자로 이루어진 군으로부터 선택된 하나 이상의 유전자를 포함하며, 자일로스(xylose)를 유일 탄소원으로 이용할 수 있는, 형질전환된 적응진화 효모 균주를 제공하는 것이다.
YALI0_A15642g 유전자에서 147G>A 돌연변이;
YALI0_A15796g 유전자에서 567_568insACA 및 576T>G 돌연변이;
YALI0_C11165g 유전자에서 1523T>C 돌연변이;
YALI0_C16247g 유전자에서 T1412A, G1441A, A1513G, A1534G, G1535T, T1544A 및 C1847T 돌연변이;
YALI0_D24849g 유전자에서 103G>A 돌연변이;
YALI0_D27016g 유전자에서 446T>C 돌연변이;
YALI0_E14388g1 유전자에서 2525C>G, 2531T>G, 2534A>G 및 2558T>C 돌연변이;
YALI0_E23969g 유전자에서 480_481insTCCTCTACCCCCGAG, 526_527insCCTCCACCACCGAGC 및 497A>C 돌연변이;
YALIO_F04906g 유전자에서 2702T>A; 또는
YALI0_F17468g 유전자에서 482_483insGCACCA 돌연변이.
다른 측면에서, 본 발명의 목적은, YALI0_A15642g, YALI0_A15796g, YALI0_C11165g, YALI0_C16247g, YALI0_D24849g, YALI0_D27016g, YALI0_E14388g1, YALI0_E23969g, YALI0_F04906g, YALI0_F17468g, YALI0_A00891g, YALI0_A00935g, YALI0_A02002g, YALI0_A02497g, YALI0_A07997g, YALI0_A13849g, YALI0_A16863g, YALI0_A17578g, YALI0_A17776g, YALI0_A17853g, YALI0_A19646g, YALI0_B00748g, YALI0_B08800g, YALI0_C06424g, YALI0_C07172g, YALI0_C08437g, YALI0_C09031g, YALI0_C09614g, YALI0_C13728g, YALI0_C14476g, YALI0_C15532g, YALI0_C16148g, YALI0_D15752g, YALI0_D17820g, YALI0_D18381g, YALI0_D19822g, YALI0_D20064g, YALI0_D20526g, YALI0_D20790g, YALI0_D24563g, YALI0_D25014g, YALI0_D25058g, YALI0_D26257g, YALI0_D26510g, YALI0_D26620g, YALI0_E07832g, YALI0_E08008g, YALI0_E11363g, YALI0_E13596g, YALI0_E16731g, YALI0_E18073g, YALI0_E18216g, YALI0_E20449g, YALIO_E21109g, YALI0_F12221g, YALI0_F12793g, YALI0_F16577g, YALI0_F19030g 및 YALI0_F23287g로 이루어진 군으로부터 선택된 하나 이상의 유전자에서 하기 돌연변이를 포함하고, D-자일로스(D-xylose)와 D-자일룰로스(D-xylulose)를 상호 변환하는 효소를 코딩하는 유전자 및 D-자일룰로스(D-xylulose)로부터 D-자일룰로스-5-인산염(D-xylulose-5-phosphate)을 생산하는 효소를 코딩하는 유전자를 포함하며, 자일로스(xylose)를 유일 탄소원으로 이용할 수 있는, 형질전환된 효모 적응진화 균주를 제공하는 것이다.
YALI0_A15642g 유전자에서 147G>A 돌연변이;
YALI0_A15796g 유전자에서 567_568insACA 및 576T>G 돌연변이;
YALI0_C11165g 유전자에서 1523T>C 돌연변이;
YALI0_C16247g 유전자에서 T1412A, G1441A, A1513G, A1534G, G1535T, T1544A 및 C1847T 돌연변이;
YALI0_D24849g 유전자에서 103G>A 돌연변이;
YALI0_D27016g 유전자에서 446T>C 돌연변이;
YALI0_E14388g1 유전자에서 2525C>G, 2531T>G, 2534A>G 및 2558T>C 돌연변이;
YALI0_E23969g 유전자에서 480_481insTCCTCTACCCCCGAG, 526_527insCCTCCACCACCGAGC 및 497A>C 돌연변이;
YALIO_F04906g 유전자에서 2702T>A;
YALI0_F17468g 유전자에서 482_483insGCACCA 돌연변이;
YALI0_A00891g 유전자에서 11_16delACGGCC 돌연변이;
YALI0_A00935g 유전자에서 202_203insGCTC 돌연변이;
YALI0_A02002g 유전자에서 1255_1256insGAGGTCAAGGTC 돌연변이;
YALI0_A02497g 유전자에서 132_137dupCAACTC 및 331_332insCCCACT 돌연변이;
YALI0_A07997g 유전자에서 2846_2847insGGAGCAGGA 및 2869_2870insAGGAGGAGG 돌연변이;
YALI0_A13849g 유전자에서 165_166insCAAA 돌연변이;
YALI0_A16863g 유전자에서 888_889insGAGCTGAGATGAC 돌연변이;
YALI0_A17578g 유전자에서 432_433insGCGGAGCCG 돌연변이;
YALI0_A17776g 유전자에서 959_960insACAGCAGAT 돌연변이;
YALI0_A17853g 유전자에서 1940_1942delAGG, 1990_1991insAGGAGGAGGCTAAGAAGA 및 2817_2818insTCTGAG 돌연변이;
YALI0_A19646g 유전자에서 1462_1463insGGG 돌연변이;
YALI0_B00748g 유전자에서 525_526insCCCGAC 돌연변이;
YALI0_B08800g 유전자에서 152_153delGT 돌연변이;
YALI0_C06424g 유전자에서 1370_1373delACTT 돌연변이;
YALI0_C07172g 유전자에서 308_309insGCAGCGACA 돌연변이;
YALI0_C08437g 유전자에서 1592_1593insGACAGTCAGCAC 돌연변이;
YALI0_C09031g 유전자에서 1789_1790insCTCCCGAGTCCTCTGCTGAGCCTACCAGCGAAGAGACTTCTTCCG 돌연변이;
YALI0_C09614g 유전자에서 1620_1622delACA 돌연변이;
YALI0_C13728g 유전자에서 99_100insAAAAAGTGGTCGAAAAAGTGGCCA 및 129_130insTGGCCGAAAAAGTGGCCAAAA 돌연변이;
YALI0_C14476g 유전자에서 778_779insTGC 돌연변이;
YALI0_C15532g 유전자에서 1611_1616delCAGCTT 돌연변이;
YALI0_C16148g 유전자에서 961_969delAGCAGCAGT 돌연변이;
YALI0_D15752g 유전자에서 1038_1039insCAG 돌연변이;
YALI0_D17820g 유전자에서 619_620insCCCACCCGCAAACCC 돌연변이;
YALI0_D18381g 유전자에서 114_115insCCTCTCACCAACTCA 돌연변이;
YALI0_D19822g 유전자에서 310_315delAAAGAG 돌연변이;
YALI0_D20064g 유전자에서 67_68insGGGGGGGG 돌연변이;
YALI0_D20526g 유전자에서 354_355insTCCACCGGA 돌연변이;
YALI0_D20790g 유전자에서 37_38insCACGTGAAAGTAGCCGAA 돌연변이;
YALI0_D24563g 유전자에서 84_85insGCT 돌연변이;
YALI0_D25014g 유전자에서 484_485insCCGCTAGCGCCAACTCTGGCTCGGAGC 돌연변이;
YALI0_D25058g 유전자에서 592_594dupAAG 돌연변이;
YALI0_D26257g 유전자에서 1478_1480delAGA돌연변이;
YALI0_D26510g 유전자에서 1287_1288insAAG 돌연변이;
YALI0_D26620g 유전자에서 926_927insCGATGAGGACGA 돌연변이;
YALI0_E07832g 유전자에서 499_500insCCAAGCCCCCCGCTTCCAAGCCCACCGCTT 돌연변이;
YALI0_E08008g 유전자에서 794_795insCTCTTCCTCTTCCTCTTCCTCTTCCTCTTC 돌연변이;
YALI0_E11363g 유전자에서 74_77delCACA 돌연변이;
YALI0_E13596g 유전자에서 1053_1054insCAACAACAACAGCAACAA 돌연변이;
YALI0_E16731g 유전자에서 1622_1623insTGAGGAGGAAGAGTAGGATGAGGAGTA 돌연변이;
YALI0_E18073g 유전자에서 266_267insCCCCACGCAGCAGTCTTG 돌연변이;
YALI0_E18216g 유전자에서 930_938delACAACAGCA 돌연변이;
YALI0_E20449g 유전자에서 899_900insAAACGC 돌연변이;
YALI0_E21109g 유전자에서 228_229insGCCCCGCCT 돌연변이;
YALI0_F12221g 유전자에서 1825_1827delAAG 돌연변이;
YALI0_F12793g 유전자에서 1855_1860delTCTTCT 돌연변이;
YALI0_F16577g 유전자에서 807_808insCCTCCT 돌연변이;
YALI0_F19030g 유전자에서 1344_1345insCCTACTACCGCCGATGTT, 2065T>A, 2098A>G 및 2099C>A 돌연변이; 또는
YALI0_F23287g 유전자에서 1919_1920insCTC 돌연변이.
일 측면에서, 본 발명은, YALI0_A15642g, YALI0_A15796g, YALI0_C11165g, YALI0_C16247g, YALI0_D24849g, YALI0_D27016g, YALI0_E14388g1, YALI0_E23969g, YALIO_F04906g 및 YALI0_F17468g로 이루어진 군으로부터 선택된 하나 이상의 유전자에서 돌연변이를 포함하고, D-자일로스(D-xylose)와 D-자일룰로스(D-xylulose)를 상호 변환하는 효소를 코딩하는 유전자 및 D-자일룰로스(D-xylulose)로부터 D-자일룰로스-5-인산염(D-xylulose-5-phosphate)을 생산하는 효소를 코딩하는 유전자로 이루어진 군으로부터 선택된 하나 이상의 유전자를 포함하며, 자일로스(xylose)를 유일 탄소원으로 이용할 수 있는, 형질전환된 적응진화 효모 균주를 제공한다.
다른 측면에서, 본 발명은, 상기 형질전환된 적응진화 효모 균주 제조방법을 제공한다.
또 다른 측면에서, 본 발명은, 상기 형질전환된 적응진화 효모 균주를 탄소원으로 자일로스를 포함하는 배지에서 배양하는 단계를 포함하는, 지질 생산 방법을 제공한다.
또 다른 측면에서, 본 발명은, 상기 형질전환된 적응진화 효모 균주를 탄소원으로 자일로스를 포함하는 배지에서 배양하여 지질을 생산하는 단계; 및 상기 생산된 지질을 트랜스에스테르화하여 바이오디젤을 수득하는 단계;를 포함하는, 바이오디젤 생산 방법을 제공한다.
본 발명에 따르면, 자일로스를 탄소원으로 이용하지 못하는 야생형 효모 균주를 자일로스 대사경로가 활성화 되도록 적응 진화시킨 후, 상기 적응 진화된 균주를 형질 전환하여 자일로스 이성화효소에 기반 한 자일로스 대사능을 부여함으로써 산환원효소 기반 자일로스 대사경로를 도입하지 않아 보조인자 불균형의 문제가 없이 지질 및 목질계 바이오매스 기반 바이오디젤과 바이오소재(화장품 등)를 높은 수율로 생산할 수 있으며, 바이오디젤 및 바이오소재 생산 공정의 경제성 및 지속가능성을 크게 향상시킬 수 있다.
도 1은 야로위아 리폴리티카(Yarrowia lipolytica) 야생형 균주(XYLA(X), XK(X))에 자일로스 이성화 효소 유전자만을 도입한 균주(XYLA(O), XK(X)), 자일룰로스 인산화효소 유전자만을 도입한 균주(XYLA(X), XK(O)), 자일로스 이성화효소와 자일룰로스 인산화효소 유전자를 모두 도입한 균주(XYLA(O), XK(O))의 자일로스를 탄소원으로 존재하는 배지에서의 성장 곡선을 나타낸 도이다.
도 2는 야로위아 리폴리티카 야생형 균주(Wild type), 및 상기 균주를 자일로스 최소배지에서 계대배양하여 자일로스 이용능을 갖는 적응진화 야로위아 리폴리티카균주(YSX)의 자일로스를 유일 탄소원으로 하는 배지에서의 성장 곡선을 나타낸 도이다.
도 3은 적응진화 야로위아 리폴리티카 균주(YSX) 및 상기 균주에 자일로스 이성화 효소 유전자만을 도입한 균주(YSX_xylA), 자일룰로스 인산화효소 유전자만을 도입한 균주(YSX_XK), 자일로스 이성화효소 유전자와 자일룰로스 인산화효소 유전자를 모두 도입한 균주(YSX_xylA_XK)의 자일로스를 탄소원으로 존재하는 최소배지에서의 성장곡선을 나타낸 도이다.
도 4는 적응 진화 과정을 거치지 않은 야로위아 리폴리티카 야생형 균주(WT) 및 형질전환된 적응진화 야로위아 리폴리티카 균주(YSX_xylA_XK)의 회분식 반응기 운전을 통하여 얻은 자일로스 전환능을 나타낸 도이다.
도 5는 회분식 반응기 운전을 통해 획득한 형질전환된 적응진화 야로위아 리폴리티카 균주(YSX_xylA_XK)의 지질 생산 농도를 적응 진화 과정을 거치지 않은 야로위아 리폴리티카 야생형 균주(WT)와 비교한 그래프이다.
이하, 본 발명을 상세히 설명한다.
본 명세서는, 점차 증가하는 바이오디젤 수요에 대응하기 위하여 기존의 식물성 기름 또는 미세조류 기반의 바이오디젤 생산의 한계를 극복할 수 있는 새로운 바이오디젤 생산기술로서, 지질을 생산하거나 내포하는 효모 균주, 구체적으로 야로위아 리폴리티카(Yarrowia lipolytica) 균주를 이용하여 바이오매스로부터 바이오디젤의 원료인 지질을 고농도로 생산하여 바이오디젤의 경제성 및 지속가능성을 향상시킬 수 있는 기술에 관한 것이다.
본 명세서에서, 야로위아 리폴리티카(Yarrowia lipolytica) 균주는 대표적인 지질 생산 균주로, 생산된 지질은 간단한 화학 반응을 거친 후 바이오디젤 및 바이오소재(화장품 원료 등)로 사용 가능하나, 야생형 야로위아 리폴리티카 균주는 자일로스를 탄소원으로 이용하지 못하는 것으로 알려져 있다. 바이오디젤 및 바이오소재 생산 시 경제성과 지속가능성을 높이기 위하여 정제당이 아닌 목질계 바이오매스를 원료로 지질을 생산할 수 있다. 상기 "목질계 바이오매스"는 지질생산 균주가 지질을 생산하는데 필요한 탄소원을 제공해 주는 원료로서 초본계 및 목본계 바이오매스를 포함하고, 지구상에서 생산되는 총 바이오매스의 90% 이상을 차지하는 가장 풍부한 탄소원이며, 친환경적으로 재생 가능한 자원이다. 또한 목질계 바이오매스를 이용하여 바이오연료를 생산할 경우 화석연료 및 1세대 바이오연료 대비 탄소 감축 효과가 크다. 이러한 목질계 바이오매스로는 폐목재, 농·임업부산물, 에너지작물 등이 있다. 한편, 목질계 바이오매스 내 가용 탄소원 중 효모와 같은 효모 균주가 이용할 수 있는 탄소원은 글루코스로, 전체 목질계 바이오매스의 약 50%를 차지한다. 만일 목질계 바이오매스 내 최대 약 30%까지 차지하고 있는 자일로스까지 이용할 수 있는 효모 균주를 개발할 경우, 목질계 바이오매스 내 가용자원을 약 80%까지 이용할 수 있기 때문에 단위 바이오매스에 대한 지질생산 수율을 크게 향상시켜 목질계 바이오매스를 이용한 바이오디젤 및 바이오소재 생산의 경제성 및 지속가능성을 크게 향상시킬 수 있다. 이에, 본 발명자는 자일로스를 탄소원으로 이용할 수 있는 효모 균주, 구체적으로 야로위아 리폴리티카 균주를 개발하였다.
일 측면에서, 본 발명은 YALI0_A15642g, YALI0_A15796g, YALI0_C11165g, YALI0_C16247g, YALI0_D24849g, YALI0_D27016g, YALI0_E14388g1, YALIO_F04906g, YALI0_E23969g 및 YALI0_F17468g로 이루어진 군으로부터 선택된 하나 이상의 유전자에서 하기 돌연변이를 포함하고, D-자일로스(D-xylose)와 D-자일룰로스(D-xylulose)를 상호 변환하는 효소를 코딩하는 유전자 및 D-자일룰로스(D-xylulose)로부터 D-자일룰로스-5-인산염(D-xylulose-5-phosphate)을 생산하는 효소를 코딩하는 유전자로 이루어진 군으로부터 선택된 하나 이상의 유전자를 포함하며, 자일로스(xylose)를 유일 탄소원으로 이용할 수 있는, 형질전환된 효모 적응진화 균주를 제공한다:
YALI0_A15642g 유전자에서 147G>A 돌연변이;
YALI0_A15796g 유전자에서 567_568insACA 및 576T>G 돌연변이;
YALI0_C11165g 유전자에서 1523T>C 돌연변이;
YALI0_C16247g 유전자에서 T1412A, G1441A, A1513G, A1534G, G1535T, T1544A 및 C1847T 돌연변이;
YALI0_D24849g 유전자에서 103G>A 돌연변이;
YALI0_D27016g 유전자에서 446T>C 돌연변이;
YALI0_E14388g1 유전자에서 2525C>G, 2531T>G, 2534A>G 및 2558T>C 돌연변이;
YALI0_E23969g 유전자에서 480_481insTCCTCTACCCCCGAG, 526_527insCCTCCACCACCGAGC 및 497A>C 돌연변이;
YALIO_F04906g 유전자에서 2702T>A; 또는
YALI0_F17468g 유전자에서 482_483insGCACCA 돌연변이.
다른 측면에서, 본 발명은 YALI0_A15642g, YALI0_A15796g, YALI0_C11165g, YALI0_C16247g, YALI0_D24849g, YALI0_D27016g, YALI0_E14388g1, YALI0_E23969g, YALI0_F04906g, YALI0_F17468g, YALI0_A00891g, YALI0_A00935g, YALI0_A02002g, YALI0_A02497g, YALI0_A07997g, YALI0_A13849g, YALI0_A16863g, YALI0_A17578g, YALI0_A17776g, YALI0_A17853g, YALI0_A19646g, YALI0_B00748g, YALI0_B08800g, YALI0_C06424g, YALI0_C07172g, YALI0_C08437g, YALI0_C09031g, YALI0_C09614g, YALI0_C13728g, YALI0_C14476g, YALI0_C15532g, YALI0_C16148g, YALI0_D15752g, YALI0_D17820g, YALI0_D18381g, YALI0_D19822g, YALI0_D20064g, YALI0_D20526g, YALI0_D20790g, YALI0_D24563g, YALI0_D25014g, YALI0_D25058g, YALI0_D26257g, YALI0_D26510g, YALI0_D26620g, YALI0_E07832g, YALI0_E08008g, YALI0_E11363g, YALI0_E13596g, YALI0_E16731g, YALI0_E18073g, YALI0_E18216g, YALI0_E20449g, YALIO_E21109g, YALI0_F12221g, YALI0_F12793g, YALI0_F16577g, YALI0_F19030g 및 YALI0_F23287g로 이루어진 군으로부터 선택된 하나 이상의 유전자에서 하기 돌연변이를 포함하고, D-자일로스(D-xylose)와 D-자일룰로스(D-xylulose)를 상호 변환하는 효소를 코딩하는 유전자 및 D-자일룰로스(D-xylulose)로부터 D-자일룰로스-5-인산염(D-xylulose-5-phosphate)을 생산하는 효소를 코딩하는 유전자로 이루어진 군으로부터 선택된 하나 이상의 유전자를 포함하며, 자일로스(xylose)를 유일 탄소원으로 이용할 수 있는, 형질전환된 효모 적응진화 균주를 제공한다:
YALI0_A15642g 유전자에서 147G>A 돌연변이;
YALI0_A15796g 유전자에서 567_568insACA 및 576T>G 돌연변이;
YALI0_C11165g 유전자에서 1523T>C 돌연변이;
YALI0_C16247g 유전자에서 T1412A, G1441A, A1513G, A1534G, G1535T, T1544A 및 C1847T 돌연변이;
YALI0_D24849g 유전자에서 103G>A 돌연변이;
YALI0_D27016g 유전자에서 446T>C 돌연변이;
YALI0_E14388g1 유전자에서 2525C>G, 2531T>G, 2534A>G 및 2558T>C 돌연변이;
YALI0_E23969g 유전자에서 480_481insTCCTCTACCCCCGAG, 526_527insCCTCCACCACCGAGC 및 497A>C 돌연변이;
YALIO_F04906g 유전자에서 2702T>A;
YALI0_F17468g 유전자에서 482_483insGCACCA 돌연변이;
YALI0_A00891g 유전자에서 11_16delACGGCC 돌연변이;
YALI0_A00935g 유전자에서 202_203insGCTC 돌연변이;
YALI0_A02002g 유전자에서 1255_1256insGAGGTCAAGGTC 돌연변이;
YALI0_A02497g 유전자에서 132_137dupCAACTC 및 331_332insCCCACT 돌연변이;
YALI0_A07997g 유전자에서 2846_2847insGGAGCAGGA 및 2869_2870insAGGAGGAGG 돌연변이;
YALI0_A13849g 유전자에서 165_166insCAAA 돌연변이;
YALI0_A16863g 유전자에서 888_889insGAGCTGAGATGAC 돌연변이;
YALI0_A17578g 유전자에서 432_433insGCGGAGCCG 돌연변이;
YALI0_A17776g 유전자에서 959_960insACAGCAGAT 돌연변이;
YALI0_A17853g 유전자에서 1940_1942delAGG, 1990_1991insAGGAGGAGGCTAAGAAGA 및 2817_2818insTCTGAG 돌연변이;
YALI0_A19646g 유전자에서 1462_1463insGGG 돌연변이;
YALI0_B00748g 유전자에서 525_526insCCCGAC 돌연변이;
YALI0_B08800g 유전자에서 152_153delGT 돌연변이;
YALI0_C06424g 유전자에서 1370_1373delACTT 돌연변이;
YALI0_C07172g 유전자에서 308_309insGCAGCGACA 돌연변이;
YALI0_C08437g 유전자에서 1592_1593insGACAGTCAGCAC 돌연변이;
YALI0_C09031g 유전자에서 1789_1790insCTCCCGAGTCCTCTGCTGAGCCTACCAGCGAAGAGACTTCTTCCG 돌연변이;
YALI0_C09614g 유전자에서 1620_1622delACA 돌연변이;
YALI0_C13728g 유전자에서 99_100insAAAAAGTGGTCGAAAAAGTGGCCA 및 129_130insTGGCCGAAAAAGTGGCCAAAA 돌연변이;
YALI0_C14476g 유전자에서 778_779insTGC 돌연변이;
YALI0_C15532g 유전자에서 1611_1616delCAGCTT 돌연변이;
YALI0_C16148g 유전자에서 961_969delAGCAGCAGT 돌연변이;
YALI0_D15752g 유전자에서 1038_1039insCAG 돌연변이;
YALI0_D17820g 유전자에서 619_620insCCCACCCGCAAACCC 돌연변이;
YALI0_D18381g 유전자에서 114_115insCCTCTCACCAACTCA 돌연변이;
YALI0_D19822g 유전자에서 310_315delAAAGAG 돌연변이;
YALI0_D20064g 유전자에서 67_68insGGGGGGGG 돌연변이;
YALI0_D20526g 유전자에서 354_355insTCCACCGGA 돌연변이;
YALI0_D20790g 유전자에서 37_38insCACGTGAAAGTAGCCGAA 돌연변이;
YALI0_D24563g 유전자에서 84_85insGCT 돌연변이;
YALI0_D25014g 유전자에서 484_485insCCGCTAGCGCCAACTCTGGCTCGGAGC 돌연변이;
YALI0_D25058g 유전자에서 592_594dupAAG 돌연변이;
YALI0_D26257g 유전자에서 1478_1480delAGA돌연변이;
YALI0_D26510g 유전자에서 1287_1288insAAG 돌연변이;
YALI0_D26620g 유전자에서 926_927insCGATGAGGACGA 돌연변이;
YALI0_E07832g 유전자에서 499_500insCCAAGCCCCCCGCTTCCAAGCCCACCGCTT 돌연변이;
YALI0_E08008g 유전자에서 794_795insCTCTTCCTCTTCCTCTTCCTCTTCCTCTTC 돌연변이;
YALI0_E11363g 유전자에서 74_77delCACA 돌연변이;
YALI0_E13596g 유전자에서 1053_1054insCAACAACAACAGCAACAA 돌연변이;
YALI0_E16731g 유전자에서 1622_1623insTGAGGAGGAAGAGTAGGATGAGGAGTA 돌연변이;
YALI0_E18073g 유전자에서 266_267insCCCCACGCAGCAGTCTTG 돌연변이;
YALI0_E18216g 유전자에서 930_938delACAACAGCA 돌연변이;
YALI0_E20449g 유전자에서 899_900insAAACGC 돌연변이;
YALI0_E21109g 유전자에서 228_229insGCCCCGCCT 돌연변이;
YALI0_F12221g 유전자에서 1825_1827delAAG 돌연변이;
YALI0_F12793g 유전자에서 1855_1860delTCTTCT 돌연변이;
YALI0_F16577g 유전자에서 807_808insCCTCCT 돌연변이;
YALI0_F19030g 유전자에서 1344_1345insCCTACTACCGCCGATGTT, 2065T>A, 2098A>G 및 2099C>A 돌연변이; 또는
YALI0_F23287g 유전자에서 1919_1920insCTC 돌연변이.
본 발명의 효모 균주 또는 야생형 효모 균주는 체내산물이나 지질을 생산할 수 있는 균주라면 그 종류가 제한되지 않으며, 구체적으로 야로위아 리폴리티카(Yarrowia lipolytica) 균주 또는 야생형 야로위아 리폴리티카 균주일 수 있다. 상기 야생형 야로위아 리폴리티카 균주는 시중에서 판매되고 있는 것일 수도 있고, 신용할 수 있는 보존기관에 보존되며 보존기관이 발행하는 카탈로그 등에 의하여 자유롭게 분양될 수 있는 사실이 확인된 것일 수 있다.
본 명세서에서, 결실(deletion, del), 삽입(insertion, ins), 프레임 시프트(frame shift, fs), 중복(duplication) 및 종결코돈(stop, *)을 포함하는 돌연변이에 대한 기재 및 이에 대한 설명은 다음과 같다. 예를 들어, "11_16delACGGCC ; p.Asn4_Gln6delinsLys"와 같은 특정 유전자의 돌연변이 표현 "A ; B"는 "A의 유전자 돌연변이로 인해 아미노산 서열이 B와 같이 변형"된 것을 의미한다. 또한, 예를 들어, "11_16delACGGCC"와 같은 특정 유전자의 돌연변이 표현은 해당 유전자의 "11번 내지 16번 염기인 ACGGCC가 결실(deletion)"된 것을 의미한다. 또한, 예를 들어, "202_203insGCTC"와 같은 특정 유전자의 돌연변이 표현은 해당 유전자의 "202번과 203번 염기 사이에 GCTC가 삽입(insertion)"된 것을 의미한다. 또한, 예를 들어, "132_137dupCAACTC"와 같은 특정 유전자의 돌연변이 표현은 해당 유전자의 "132번 내지 137번 염기인 CAACTC가 중복(duplication)"된 것을 의미한다. 또한, 예를 들어, "147G>A"와 같은 특정 유전자의 돌연변이 표현은 해당 유전자의 "147번 염기인 G가 A로 치환"된 것을 의미한다. 또한, 예를 들어, 특정 유전자의 돌연변이로 인한 "p.Trp49*"와 같은 아미노산 서열 변형(돌연변이) 표현은 "아미노산 Trp이 종결코돈(stop, *)으로 바뀐" 것을 의미한다. 또한, 예를 들어, "T1412A"와 같은 특정 유전자의 돌연변이 표현은 해당 유전자의 "1412번 염기인 T가 A로 치환"된 것을 의미한다.
본 명세서에서, 용어 "적응진화 효모 균주"는 YALI0_A15642g, YALI0_A15796g, YALI0_C11165g, YALI0_C16247g, YALI0_D24849g, YALI0_D27016g, YALI0_E14388g1, YALI0_E23969g, YALIO_F04906g 및 YALI0_F17468g 로 이루어진 군으로부터 선택된 하나 이상의 유전자에서 하기와 같은 돌연변이를 포함하는 균주일 수 있다:
YALI0_A15642g 유전자에서 147G>A 돌연변이;
YALI0_A15796g 유전자에서 567_568insACA 및 576T>G 돌연변이;
YALI0_C11165g 유전자에서 1523T>C 돌연변이;
YALI0_C16247g 유전자에서 T1412A, G1441A, A1513G, A1534G, G1535T, T1544A 및 C1847T 돌연변이;
YALI0_D24849g 유전자에서 103G>A 돌연변이;
YALI0_D27016g 유전자에서 446T>C 돌연변이;
YALI0_E14388g1 유전자에서 2525C>G, 2531T>G, 2534A>G 및 2558T>C 돌연변이;
YALI0_E23969g 유전자에서 480_481insTCCTCTACCCCCGAG, 526_527insCCTCCACCACCGAGC 및 497A>C 돌연변이;
YALIO_F04906g 유전자에서 2702T>A; 또는
YALI0_F17468g 유전자에서 482_483insGCACCA 돌연변이.
또는, 본 명세서에서 용어, "적응진화 효모 균주"는 YALI0_A15642g, YALI0_A15796g, YALI0_C11165g, YALI0_C16247g, YALI0_D24849g, YALI0_D27016g, YALI0_E14388g1, YALI0_E23969g, YALI0_F04906g, YALI0_F17468g, YALI0_A00891g, YALI0_A00935g, YALI0_A02002g, YALI0_A02497g, YALI0_A07997g, YALI0_A13849g, YALI0_A16863g, YALI0_A17578g, YALI0_A17776g, YALI0_A17853g, YALI0_A19646g, YALI0_B00748g, YALI0_B08800g, YALI0_C06424g, YALI0_C07172g, YALI0_C08437g, YALI0_C09031g, YALI0_C09614g, YALI0_C13728g, YALI0_C14476g, YALI0_C15532g, YALI0_C16148g, YALI0_D15752g, YALI0_D17820g, YALI0_D18381g, YALI0_D19822g, YALI0_D20064g, YALI0_D20526g, YALI0_D20790g, YALI0_D24563g, YALI0_D25014g, YALI0_D25058g, YALI0_D26257g, YALI0_D26510g, YALI0_D26620g, YALI0_E07832g, YALI0_E08008g, YALI0_E11363g, YALI0_E13596g, YALI0_E16731g, YALI0_E18073g, YALI0_E18216g, YALI0_E20449g, YALIO_E21109g, YALI0_F12221g, YALI0_F12793g, YALI0_F16577g, YALI0_F19030g 및 YALI0_F23287g로 이루어진 군으로부터 선택된 하나 이상의 유전자에서 하기와 같은 돌연변이를 포함하는 균주일 수 있다:
YALI0_A15642g 유전자에서 147G>A 돌연변이;
YALI0_A15796g 유전자에서 567_568insACA 및 576T>G 돌연변이;
YALI0_C11165g 유전자에서 1523T>C 돌연변이;
YALI0_C16247g 유전자에서 T1412A, G1441A, A1513G, A1534G, G1535T, T1544A 및 C1847T 돌연변이;
YALI0_D24849g 유전자에서 103G>A 돌연변이;
YALI0_D27016g 유전자에서 446T>C 돌연변이;
YALI0_E14388g1 유전자에서 2525C>G, 2531T>G, 2534A>G 및 2558T>C 돌연변이;
YALI0_E23969g 유전자에서 480_481insTCCTCTACCCCCGAG, 526_527insCCTCCACCACCGAGC 및 497A>C 돌연변이;
YALIO_F04906g 유전자에서 2702T>A;
YALI0_F17468g 유전자에서 482_483insGCACCA 돌연변이;
YALI0_A00891g 유전자에서 11_16delACGGCC 돌연변이;
YALI0_A00935g 유전자에서 202_203insGCTC 돌연변이;
YALI0_A02002g 유전자에서 1255_1256insGAGGTCAAGGTC 돌연변이;
YALI0_A02497g 유전자에서 132_137dupCAACTC 및 331_332insCCCACT 돌연변이;
YALI0_A07997g 유전자에서 2846_2847insGGAGCAGGA 및 2869_2870insAGGAGGAGG 돌연변이;
YALI0_A13849g 유전자에서 165_166insCAAA 돌연변이;
YALI0_A16863g 유전자에서 888_889insGAGCTGAGATGAC 돌연변이;
YALI0_A17578g 유전자에서 432_433insGCGGAGCCG 돌연변이;
YALI0_A17776g 유전자에서 959_960insACAGCAGAT 돌연변이;
YALI0_A17853g 유전자에서 1940_1942delAGG, 1990_1991insAGGAGGAGGCTAAGAAGA 및 2817_2818insTCTGAG 돌연변이;
YALI0_A19646g 유전자에서 1462_1463insGGG 돌연변이;
YALI0_B00748g 유전자에서 525_526insCCCGAC 돌연변이;
YALI0_B08800g 유전자에서 152_153delGT 돌연변이;
YALI0_C06424g 유전자에서 1370_1373delACTT 돌연변이;
YALI0_C07172g 유전자에서 308_309insGCAGCGACA 돌연변이;
YALI0_C08437g 유전자에서 1592_1593insGACAGTCAGCAC 돌연변이;
YALI0_C09031g 유전자에서 1789_1790insCTCCCGAGTCCTCTGCTGAGCCTACCAGCGAAGAGACTTCTTCCG 돌연변이;
YALI0_C09614g 유전자에서 1620_1622delACA 돌연변이;
YALI0_C13728g 유전자에서 99_100insAAAAAGTGGTCGAAAAAGTGGCCA 및 129_130insTGGCCGAAAAAGTGGCCAAAA 돌연변이;
YALI0_C14476g 유전자에서 778_779insTGC 돌연변이;
YALI0_C15532g 유전자에서 1611_1616delCAGCTT 돌연변이;
YALI0_C16148g 유전자에서 961_969delAGCAGCAGT 돌연변이;
YALI0_D15752g 유전자에서 1038_1039insCAG 돌연변이;
YALI0_D17820g 유전자에서 619_620insCCCACCCGCAAACCC 돌연변이;
YALI0_D18381g 유전자에서 114_115insCCTCTCACCAACTCA 돌연변이;
YALI0_D19822g 유전자에서 310_315delAAAGAG 돌연변이;
YALI0_D20064g 유전자에서 67_68insGGGGGGGG 돌연변이;
YALI0_D20526g 유전자에서 354_355insTCCACCGGA 돌연변이;
YALI0_D20790g 유전자에서 37_38insCACGTGAAAGTAGCCGAA 돌연변이;
YALI0_D24563g 유전자에서 84_85insGCT 돌연변이;
YALI0_D25014g 유전자에서 484_485insCCGCTAGCGCCAACTCTGGCTCGGAGC 돌연변이;
YALI0_D25058g 유전자에서 592_594dupAAG 돌연변이;
YALI0_D26257g 유전자에서 1478_1480delAGA돌연변이;
YALI0_D26510g 유전자에서 1287_1288insAAG 돌연변이;
YALI0_D26620g 유전자에서 926_927insCGATGAGGACGA 돌연변이;
YALI0_E07832g 유전자에서 499_500insCCAAGCCCCCCGCTTCCAAGCCCACCGCTT 돌연변이;
YALI0_E08008g 유전자에서 794_795insCTCTTCCTCTTCCTCTTCCTCTTCCTCTTC 돌연변이;
YALI0_E11363g 유전자에서 74_77delCACA 돌연변이;
YALI0_E13596g 유전자에서 1053_1054insCAACAACAACAGCAACAA 돌연변이;
YALI0_E16731g 유전자에서 1622_1623insTGAGGAGGAAGAGTAGGATGAGGAGTA 돌연변이;
YALI0_E18073g 유전자에서 266_267insCCCCACGCAGCAGTCTTG 돌연변이;
YALI0_E18216g 유전자에서 930_938delACAACAGCA 돌연변이;
YALI0_E20449g 유전자에서 899_900insAAACGC 돌연변이;
YALI0_E21109g 유전자에서 228_229insGCCCCGCCT 돌연변이;
YALI0_F12221g 유전자에서 1825_1827delAAG 돌연변이;
YALI0_F12793g 유전자에서 1855_1860delTCTTCT 돌연변이;
YALI0_F16577g 유전자에서 807_808insCCTCCT 돌연변이;
YALI0_F19030g 유전자에서 1344_1345insCCTACTACCGCCGATGTT, 2065T>A, 2098A>G 및 2099C>A 돌연변이; 또는
YALI0_F23287g 유전자에서 1919_1920insCTC 돌연변이.
상기 돌연변이는 기술분야에서 돌연변이를 유발할 수 있는 것으로서 알려져 있는 임의의 화학적 수단 및/또는 물리적 수단을 미생물에 처리함으로써 도입될 수 있다. 상기 화학적 수단은 돌연변이를 유발하는 물질(돌연변이원)로써 유효한 구아니딘 유도체인 NTG(nitrosoguanidine), MMS(methyl methanesulfonate), EMS(ethyl methanesulfonate), 벤조피렌 등과 같은 화학물질을 들 수 있고, 상기 물리적 수단은 자외선, X-선, γ-선 등과 같은 방사선을 들 수 있으나 이에 한정되는 것은 아니다. 또한 상기 돌연변이 유전자는 분자생물학적 방법을 이용하여 도입될 수 있으며, 구체적으로 유전자 가위, 예를 들어 CRISPR-Cas를 이용할 수 있으나 이에 제한되는것은 아니다. 상기 적응진화 효모 균주는 자일로스를 유일 탄소원으로 하는 배지에서 3회 이상, 구체적으로 3회 이상, 4회 이상 또는 5회 이상 계대배양하여 자일로스 이용능을 갖도록 적응 진화된 돌연변이 균주일 수 있으나, 이에 제한되는 것은 아니다. 또는, 상기 적응진화 효모 균주는 야로위아 리폴리티카 적응진화 균주일 수 있고, 기탁번호 KCTC13615BP인 균주일 수 있다.
본 발명의 형질전환된 적응진화 효모 균주는 상기 적응진화 효모 균주를 형질전환시킨 균주일 수 있고, 형질전환된 야로위아 리폴리티카 적응진화 균주일 수 있으며, 구체적으로 D-자일로스(D-xylose)와 D-자일룰로스(D-xylulose)를 상호 변환하는 효소를 코딩하는 유전자 및 D-자일룰로스(D-xylulose)로부터 D-자일룰로스-5-인산염(D-xylulose-5-phosphate)을 생산하는 효소를 코딩하는 유전자로 이루어진 군으로부터 선택된 하나 이상의 유전자를 포함하도록 형질전환시킨 균주일 수 있다.
본 발명의 일 실시예에 따르면, 야생형 야로위아 리폴리티카 균주를 자일로스가 유일 탄소원인 배지에서 5회 계대배양 시, 하기 표 1의 유전자로 이루어진 군으로부터 선택된 하나 이상의 유전자가 하기 표 1과 같이 돌연변이된 적응진화 균주(YSK)는 자일로스 이성화효소를 발현하는 야생형 야로위아 리폴리티카 균주에 비해 자일로스 이용능이 매우 향상되어 자일로스를 유일 탄소원으로 하여 성장할 수 있음을 확인하였다(실시예 2). 하기 표 1의 각 서열번호의 염기서열은 각 유전자가 하기 표 1과 같이 돌연변이된 염기서열을 나타낸다.
유전자 No. 유전자명 NCBI
Reference Sequence no. 		돌연변이 서열
번호
1 YALI0_A00891g NC_006067.1 (112988..114379, complement) 11_16delACGGCC ; p.Asn4_Gln6delinsLys 5
2 YALI0_A00935g NC_006067.1 (121112..121432, complement) 202_203insGCTC ; p.Tyr68fs 6
3 YALI0_A02002g NC_006067.1 (252726..254057, complement) 1255_1256insGAGGTCAAGGTC ; p.Gly418_Gln419insArgGlyGlnGly 7
4 YALI0_A02497g NC_006067.1 (316093..317005, complement) 132_137dupCAACTC ; p.Ser46_Gly47insAsnSer, 331_332insCCCACT ; p.Asn110_Tyr111insSerHis 8
5 YALI0_A07997g NC_006067.1 (762324..766571, complement) 2846_2847insGGAGCAGGA ;p.Gly949_Glu950insGluGlnGlu, 2869_2870insAGGAGGAGG ; p.Lys957_Glu958insGluGluGlu 9
6 YALI0_A13849g NC_006067.1 (1420547..1420819, complement) 165_166insCAAA ; p.Lys56fs 10
7 YALI0_A15642g NC_006067.1 (1605399..1605629, complement) 147G>A ; p.Trp49* 11
8 YALI0_A15796g NC_006067.1 (1616617..1619565, complement) 567_568insACA ; p.Thr189dup, 576T>G ; p.Asp192Glu 12
9 YALI0_A16863g NC_006067.1 (1743518..1744417, complement) 888_889insGAGCTGAGATGAC ; p.Val298fs 13
10 YALI0_A17578g NC_006067.1 (1813242..1816943, complement) 432_433insGCGGAGCCG ; p.Pro144_Ser145insAlaGluPro 14
11 YALI0_A17776g NC_006067.1 (1825648..1829883, complement) 959_960insACAGCAGAT ; p.Pro320_Gln321insGlnGlnIle 15
12 YALI0_A17853g NC_006067.1 (1845367..1849173, complement) 1940_1942delAGG ; p.Glu648del, 1990_1991insAGGAGGAGGCTAAGAAGA ;
p.Glu664_Glu665insGluGluAlaLysLysLys, 2817_2818insTCTGAG ; p.Asp939_Ser940insSerGlu 		16
13 YALI0_A19646g NC_006067.1 (2112864..2114768, complement) 1462_1463insGGG ; p.Glu487_Glu488insGly 17
14 YALI0_B00748g NC_006068.1 (102187..103176, complement) 525_526insCCCGAC ; p.Asp175_Ser176insProAsp 18
15 YALI0_B08800g NC_006068.1 (1210467..1212137, complement) 152_153delGT ; p.Cys51fs 19
16 YALI0_C06424g NC_006069.1 (852842..854389, complement) 1370_1373delACTT ; p.Tyr457fs 20
17 YALI0_C07172g NC_0060697.1 (955882..958353, complement) 308_309insGCAGCGACA ; p.Gln103_Gln104insGlnArgGln 21
18 YALI0_C08437g NC_006069.1 (1146546..1148330, complement) 1592_1593insGACAGTCAGCAC ; p.Lys531_Thr532insThrValSerThr 22
19 YALI0_C09031g NC_006069.1 (1232749..1236846, complement) 1789_1790insCTCCCGAGTCCTCTGCTGAGCCTACCAGCGAAGAGACTTCTTCCG; p.Ser596_Val597insAlaProGluSerSerAlaGluProThrSerGluGluThrSerSer 23
20 YALI0_C09614g NC_006069.1 (1309626..1312265, complement) 1620_1622delACA ; p.Gln541del 24
21 YALI0_C11165g NC_006069.1 (1536684..1540028, complement) 1523T>C ; p.Val508Ala 25
22 YALI0_C13728g NC_006069.1 (1885929..1886756, complement) 99_100insAAAAAGTGGTCGAAAAAGTGGCCA ;p.Pro33_Lys34insLysLysTrpSerLysLysTrpPro, 129_130insTGGCCGAAAAAGTGGCCAAAA ;
p.Lys43_Lys44insTrpProLysLysTrpProLys		 26
23 YALI0_C14476g NC_006069.1 (1992000..1994063, complement) 778_779insTGC ; p.Lys260delinsMetGln 27
24 YALI0_C15532g NC_006069.1 (2170451..2173659, complement) 1611_1616delCAGCTT ; p.Ser538_Phe539del 28
25 YALI0_C16148g NC_006069.1 (2285288..2286607, complement) 961_969delAGCAGCAGT ; p.Ser321_Ser323del 29
26 YALI0_C16247g NC_006069.1 (2295675..2299085, complement) T1412A ; Val471Asp, G1441A ; Asp481Asn, A1513G ; Asn505Asp, A1534G ; Ser512Gly, G1535T ;
Ser512Ile, T1544A ; Val515Glu, C1847T ; Ala616Val 		30
27 YALI0_D15752g NC_006070.1 (1928726..1930150, complement) 1038_1039insCAG ; Pro346_Gln347insGln 31
28 YALI0_D17820g NC_006070.1 (2203609..2206944, complement) 619_620insCCCACCCGCAAACCC ;Thr206_His207insProHisProGlnThr 32
29 YALI0_D18381g NC_006070.1 (2301563..2304454, complement) 114_115insCCTCTCACCAACTCA ; Ser38_Thr39insProLeuThrAsnSer 33
30 YALI0_D19822g NC_006070.1 (2505960..2508386, complement) 310_315delAAAGAG ; Lys104_Glu105del 34
31 YALI0_D20064g NC_006070.1 (2529127..2530611, complement) 67_68insGGGGGGGG ; p.Tyr23fs 35
32 YALI0_D20526g NC_006070.1 (2604298..2604907, complement) 354_355insTCCACCGGA ; p.Gly118_Ser119insSerThrGly 36
33 YALI0_D20790g(Pre-mRNA-splicing factor CWC22) NC_006070.1 (2636519..2639383, complement) 37_38insCACGTGAAAGTAGCCGAA ;
p.Ser13_Arg14insArgGluSerSerArgThr 		37
34 YALI0_D24563g NC_006070.1 (3267394..3268023, complement) 84_85insGCT ; p.Glu28_Lys29insAla 38
35 YALI0_D24849g NC_006070.1 (3309927..3310196, complement) 103G>A ; p.Ala35Thr 39
36 YALI0_D25014g NC_006070.1 (3333236..3334741, complement) 484_485insCCGCTAGCGCCAACTCTGGCTCGGAGC ;p.Ala162_Ala163insAlaSerAlaAsnSerGlySerGluPro 40
37 YALI0_D25058g(FACT complex subunit POB3) NC_006070.1 (3340885..3342519, complement) 592_594dupAAG ; p.Lys198dup 41
38 YALI0_D26257g NC_006070.1 (3490069..3492042, complement) 1478_1480delAGA ; p.Glu493_Thr494delinsAla ; 42
39 YALI0_D26510g NC_006070.1 (3533723..3535557, complement) 1287_1288insAAG ; p.Gln429_Gln430insLys 43
40 YALI0_D26620g NC_006070.1 (3542396..3546277, complement) 926_927insCGATGAGGACGA ; p.Asp308_Glu309insAspAspGluAsp 44
41 YALI0_D27016g NC_006070.1 (3595010..3597181, complement) 446T>C ; p.Leu149Pro 45
42 YALI0_E07832g NC_006071.1 (908935..909591, complement) 499_500insCCAAGCCCCCCGCTTCCAAGCCCACCGCTT ;p.Pro167_Lys168insLysProProAlaSerLysProThrAlaSer 46
43 YALI0_E08008g NC_006071.1 (934849..936210, complement) 794_795insCTCTTCCTCTTCCTCTTCCTCTTCCTCTTC ;p.Gly265_Ser266insSerSerSerSerSerSerSerSerSerSer 47
44 YALI0_E11363g NC_006071.1 (1388910..1390034, complement) 74_77delCACA ; p.Thr25fs 48
45 YALI0_E13596g NC_006071.1 (1643634..1645772, complement) 1053_1054insCAACAACAACAGCAACAA ; p.Gln346_Gln351dup 49
46 YALI0_E14388g1 NC_006071.1 (1723970..1728435, complement) 2531T>G ; p.Leu844Arg, 2534A>G ; p.Gln845Arg, 2558T>C ; p.Val853Ala, 2525C>G ; p.Ser842* 50
47 YALI0_E16731g NC_006071.1 (1979332..1982976, complement) 1622_1623insTGAGGAGGAAGAGTAGGATGAGGAGTA ; p.Glu541delinsAspGluGluGluGluTerAspGluGluTer 51
48 YALI0_E18073g NC_006071.1 (2132735..2133649, complement) 266_267insCCCCACGCAGCAGTCTTG ; p.Cys89_Pro90insProThrGlnGlnSerCys 52
49 YALI0_E18216g NC_006071.1 (2171557..2172525, complement) 930_938delACAACAGCA ; p.Gln311_Gln313del 53
50 YALI0_E20449g NC_006071.1 (2423925..2425388, complement) 899_900insAAACGC ; p.Ala300_Asn301insAsnAla 54
51 YALI0_E21109g NC_006071.1 (2510568..2511743, complement) 228_229insGCCCCGCCT ; p.Gln76_Ala77insAlaProPro 55
52 YALI0_E23969g NC_0060717.1 (2842397..2843512, complement) 480_481insTCCTCTACCCCCGAG ; p.Ser160_Ser161insSerSerThrProGlu, 526_527insCCTCCACCACCGAGC ;
p.Ser176_Ser177insSerThrThrGluPro,
497A>C ; p.Tyr166Ser 		56
53 YALI0_F04906g NC_006072.1 (750999..754226, complement) 2702T>A ; p.Val901Glu 57
54 YALI0_F12221g NC_006072.1 (1609086..1611512, complement) 1825_1827delAAG ; p.Lys609del 58
55 YALI0_F12793g NC_0060727.1 (1713697..1718010, complement) 1855_1860delTCTTCT ; p.Ser619_Ser620del 59
56 YALI0_F16577g NC_006072.1 (2216900..2218064, complement) 807_808insCCTCCT ; p.Leu269_Thr270insProPro 60
57 YALI0_F17468g NC_006072.1 (2332587..2335994, complement) 482_483insGCACCA ; p.Gln160_His161insGlnHis 61
58 YALI0_F19030g NC_006072.1 (2541990..2544461, complement) 1344_1345insCCTACTACCGCCGATGTT ; p.Val448_Pro449insProThrThrAlaAspVal, 2065T>A ; p.Ser689Thr, 2098A>G ; p.Thr700Ala, 2099C>A ; p.Thr700Asn 62
59 YALI0_F23287g NC_006072.1 (3052893..3056228, complement) 1919_1920insCTC ; p.Ser640dup 63
del: 결실(deletion), ins: 삽입(insertion), fs: 프레임 시프트(frame shift),
dup: 중복(duplication), *: stop
본 발명의 형질전환된 적응진화 효모 균주는 D-자일로스와 D-자일룰로스를 상호 변환하는 효소를 코딩하는 유전자를 포함할 수 있다. 또는, 본 발명의 형질전환된 적응진화 효모 균주는 YALI0_A15642g, YALI0_A15796g, YALI0_C11165g, YALI0_C16247g, YALI0_D24849g, YALI0_D27016g, YALI0_E14388g1, YALI0_E23969g, YALIO_F04906g 및 YALI0_F17468g로 이루어진 군으로부터 선택된 하나 이상의 유전자에서
YALI0_A15642g 유전자에서 147G>A 돌연변이;
YALI0_A15796g 유전자에서 567_568insACA 및 576T>G 돌연변이;
YALI0_C11165g 유전자에서 1523T>C 돌연변이;
YALI0_C16247g 유전자에서 T1412A, G1441A, A1513G, A1534G, G1535T, T1544A 및 C1847T 돌연변이;
YALI0_D24849g 유전자에서 103G>A 돌연변이;
YALI0_D27016g 유전자에서 446T>C 돌연변이;
YALI0_E14388g1 유전자에서 2525C>G, 2531T>G, 2534A>G 및 2558T>C 돌연변이;
YALI0_E23969g 유전자에서 480_481insTCCTCTACCCCCGAG, 526_527insCCTCCACCACCGAGC 및 497A>C 돌연변이;
YALIO_F04906g 유전자에서 2702T>A; 또는
YALI0_F17468g 유전자에서 482_483insGCACCA 돌연변이;를 포함하거나, 또는
YALI0_A15642g, YALI0_A15796g, YALI0_C11165g, YALI0_C16247g, YALI0_D24849g, YALI0_D27016g, YALI0_E14388g1, YALI0_E23969g, YALI0_F04906g, YALI0_F17468g, YALI0_A00891g, YALI0_A00935g, YALI0_A02002g, YALI0_A02497g, YALI0_A07997g, YALI0_A13849g, YALI0_A16863g, YALI0_A17578g, YALI0_A17776g, YALI0_A17853g, YALI0_A19646g, YALI0_B00748g, YALI0_B08800g, YALI0_C06424g, YALI0_C07172g, YALI0_C08437g, YALI0_C09031g, YALI0_C09614g, YALI0_C13728g, YALI0_C14476g, YALI0_C15532g, YALI0_C16148g, YALI0_D15752g, YALI0_D17820g, YALI0_D18381g, YALI0_D19822g, YALI0_D20064g, YALI0_D20526g, YALI0_D20790g, YALI0_D24563g, YALI0_D25014g, YALI0_D25058g, YALI0_D26257g, YALI0_D26510g, YALI0_D26620g, YALI0_E07832g, YALI0_E08008g, YALI0_E11363g, YALI0_E13596g, YALI0_E16731g, YALI0_E18073g, YALI0_E18216g, YALI0_E20449g, YALIO_E21109g, YALI0_F12221g, YALI0_F12793g, YALI0_F16577g, YALI0_F19030g 및 YALI0_F23287g로 이루어진 군으로부터 선택된 하나 이상의 유전자에서
YALI0_A15642g 유전자에서 147G>A 돌연변이;
YALI0_A15796g 유전자에서 567_568insACA 및 576T>G 돌연변이;
YALI0_C11165g 유전자에서 1523T>C 돌연변이;
YALI0_C16247g 유전자에서 T1412A, G1441A, A1513G, A1534G, G1535T, T1544A 및 C1847T 돌연변이;
YALI0_D24849g 유전자에서 103G>A 돌연변이;
YALI0_D27016g 유전자에서 446T>C 돌연변이;
YALI0_E14388g1 유전자에서 2525C>G, 2531T>G, 2534A>G 및 2558T>C 돌연변이;
YALI0_E23969g 유전자에서 480_481insTCCTCTACCCCCGAG, 526_527insCCTCCACCACCGAGC 및 497A>C 돌연변이;
YALIO_F04906g 유전자에서 2702T>A;
YALI0_F17468g 유전자에서 482_483insGCACCA 돌연변이;
YALI0_A00891g 유전자에서 11_16delACGGCC 돌연변이;
YALI0_A00935g 유전자에서 202_203insGCTC 돌연변이;
YALI0_A02002g 유전자에서 1255_1256insGAGGTCAAGGTC 돌연변이;
YALI0_A02497g 유전자에서 132_137dupCAACTC 및 331_332insCCCACT 돌연변이;
YALI0_A07997g 유전자에서 2846_2847insGGAGCAGGA 및 2869_2870insAGGAGGAGG 돌연변이;
YALI0_A13849g 유전자에서 165_166insCAAA 돌연변이;
YALI0_A16863g 유전자에서 888_889insGAGCTGAGATGAC 돌연변이;
YALI0_A17578g 유전자에서 432_433insGCGGAGCCG 돌연변이;
YALI0_A17776g 유전자에서 959_960insACAGCAGAT 돌연변이;
YALI0_A17853g 유전자에서 1940_1942delAGG, 1990_1991insAGGAGGAGGCTAAGAAGA 및 2817_2818insTCTGAG 돌연변이;
YALI0_A19646g 유전자에서 1462_1463insGGG 돌연변이;
YALI0_B00748g 유전자에서 525_526insCCCGAC 돌연변이;
YALI0_B08800g 유전자에서 152_153delGT 돌연변이;
YALI0_C06424g 유전자에서 1370_1373delACTT 돌연변이;
YALI0_C07172g 유전자에서 308_309insGCAGCGACA 돌연변이;
YALI0_C08437g 유전자에서 1592_1593insGACAGTCAGCAC 돌연변이;
YALI0_C09031g 유전자에서 1789_1790insCTCCCGAGTCCTCTGCTGAGCCTACCAGCGAAGAGACTTCTTCCG 돌연변이;
YALI0_C09614g 유전자에서 1620_1622delACA 돌연변이;
YALI0_C13728g 유전자에서 99_100insAAAAAGTGGTCGAAAAAGTGGCCA 및 129_130insTGGCCGAAAAAGTGGCCAAAA 돌연변이;
YALI0_C14476g 유전자에서 778_779insTGC 돌연변이;
YALI0_C15532g 유전자에서 1611_1616delCAGCTT 돌연변이;
YALI0_C16148g 유전자에서 961_969delAGCAGCAGT 돌연변이;
YALI0_D15752g 유전자에서 1038_1039insCAG 돌연변이;
YALI0_D17820g 유전자에서 619_620insCCCACCCGCAAACCC 돌연변이;
YALI0_D18381g 유전자에서 114_115insCCTCTCACCAACTCA 돌연변이;
YALI0_D19822g 유전자에서 310_315delAAAGAG 돌연변이;
YALI0_D20064g 유전자에서 67_68insGGGGGGGG 돌연변이;
YALI0_D20526g 유전자에서 354_355insTCCACCGGA 돌연변이;
YALI0_D20790g 유전자에서 37_38insCACGTGAAAGTAGCCGAA 돌연변이;
YALI0_D24563g 유전자에서 84_85insGCT 돌연변이;
YALI0_D25014g 유전자에서 484_485insCCGCTAGCGCCAACTCTGGCTCGGAGC 돌연변이;
YALI0_D25058g 유전자에서 592_594dupAAG 돌연변이;
YALI0_D26257g 유전자에서 1478_1480delAGA, 돌연변이;
YALI0_D26510g 유전자에서 1287_1288insAAG 돌연변이;
YALI0_D26620g 유전자에서 926_927insCGATGAGGACGA 돌연변이;
YALI0_E07832g 유전자에서 499_500insCCAAGCCCCCCGCTTCCAAGCCCACCGCTT 돌연변이;
YALI0_E08008g 유전자에서 794_795insCTCTTCCTCTTCCTCTTCCTCTTCCTCTTC 돌연변이;
YALI0_E11363g 유전자에서 74_77delCACA 돌연변이;
YALI0_E13596g 유전자에서 1053_1054insCAACAACAACAGCAACAA 돌연변이;
YALI0_E16731g 유전자에서 1622_1623insTGAGGAGGAAGAGTAGGATGAGGAGTA 돌연변이;
YALI0_E18073g 유전자에서 266_267insCCCCACGCAGCAGTCTTG 돌연변이;
YALI0_E18216g 유전자에서 930_938delACAACAGCA 돌연변이;
YALI0_E20449g 유전자에서 899_900insAAACGC 돌연변이;
YALI0_E21109g 유전자에서 228_229insGCCCCGCCT 돌연변이;
YALI0_F12221g 유전자에서 1825_1827delAAG 돌연변이;
YALI0_F12793g 유전자에서 1855_1860delTCTTCT 돌연변이;
YALI0_F16577g 유전자에서 807_808insCCTCCT 돌연변이;
YALI0_F19030g 유전자에서 1344_1345insCCTACTACCGCCGATGTT, 2065T>A, 2098A>G 및 2099C>A 돌연변이; 또는
YALI0_F23287g 유전자에서 1919_1920insCTC 돌연변이;
를 포함하고, D-자일로스와 D-자일룰로스를 상호 변환하는 효소를 코딩하는 유전자를 포함할 수 있다. 상기 D-자일로스와 D-자일룰로스를 상호 변환하는 효소를 코딩하는 유전자는 자일로스 이성화효소를 코딩하는 유전자(xylose isomerase)일 수 있다. 상기 D-자일로스와 D-자일룰로스를 상호 변환하는 효소를 코딩하는 유전자는 피로마이세스 종(Piromyces sp.)으로부터 유래한 것일 수 있고, 구체적으로 xylA (xylose isomerase) 유전자일 수 있으며, 보다 구체적으로 피로마이세스 종(Piromyces sp.)으로부터 유래한 xylA (xylose isomerase) 유전자가 성능이 향상되도록 돌연변이된 유전자(xylA3*)일 수 있으며, 보다 더 구체적으로 서열번호 1의 염기서열로 이루어진 유전자일 수 있다.
본 발명의 형질전환된 적응진화 효모 균주는 D-자일룰로스(D-xylulose)로부터 D-자일룰로스-5-인산염(D-xylulose-5-phosphate)을 생산하는 효소를 코딩하는 유전자를 포함할 수 있다. 또는, 본 발명의 형질전환된 적응진화 효모 균주는YALI0_A15642g, YALI0_A15796g, YALI0_C11165g, YALI0_C16247g, YALI0_D24849g, YALI0_D27016g, YALI0_E14388g1, YALI0_E23969g, YALIO_F04906g 및 YALI0_F17468g로 이루어진 군으로부터 선택된 하나 이상의 유전자에서
YALI0_A15642g 유전자에서 147G>A 돌연변이;
YALI0_A15796g 유전자에서 567_568insACA 및 576T>G 돌연변이;
YALI0_C11165g 유전자에서 1523T>C 돌연변이;
YALI0_C16247g 유전자에서 T1412A, G1441A, A1513G, A1534G, G1535T, T1544A 및 C1847T 돌연변이;
YALI0_D24849g 유전자에서 103G>A 돌연변이;
YALI0_D27016g 유전자에서 446T>C 돌연변이;
YALI0_E14388g1 유전자에서 2525C>G, 2531T>G, 2534A>G 및 2558T>C 돌연변이;
YALI0_E23969g 유전자에서 480_481insTCCTCTACCCCCGAG, 526_527insCCTCCACCACCGAGC 및 497A>C 돌연변이;
YALIO_F04906g 유전자에서 2702T>A; 또는
YALI0_F17468g 유전자에서 482_483insGCACCA 돌연변이;를 포함하거나, 또는
YALI0_A15642g, YALI0_A15796g, YALI0_C11165g, YALI0_C16247g, YALI0_D24849g, YALI0_D27016g, YALI0_E14388g1, YALI0_E23969g, YALI0_F04906g, YALI0_F17468g, YALI0_A00891g, YALI0_A00935g, YALI0_A02002g, YALI0_A02497g, YALI0_A07997g, YALI0_A13849g, YALI0_A16863g, YALI0_A17578g, YALI0_A17776g, YALI0_A17853g, YALI0_A19646g, YALI0_B00748g, YALI0_B08800g, YALI0_C06424g, YALI0_C07172g, YALI0_C08437g, YALI0_C09031g, YALI0_C09614g, YALI0_C13728g, YALI0_C14476g, YALI0_C15532g, YALI0_C16148g, YALI0_D15752g, YALI0_D17820g, YALI0_D18381g, YALI0_D19822g, YALI0_D20064g, YALI0_D20526g, YALI0_D20790g, YALI0_D24563g, YALI0_D25014g, YALI0_D25058g, YALI0_D26257g, YALI0_D26510g, YALI0_D26620g, YALI0_E07832g, YALI0_E08008g, YALI0_E11363g, YALI0_E13596g, YALI0_E16731g, YALI0_E18073g, YALI0_E18216g, YALI0_E20449g, YALIO_E21109g, YALI0_F12221g, YALI0_F12793g, YALI0_F16577g, YALI0_F19030g 및 YALI0_F23287g로 이루어진 군으로부터 선택된 하나 이상의 유전자에서
YALI0_A15642g 유전자에서 147G>A 돌연변이;
YALI0_A15796g 유전자에서 567_568insACA 및 576T>G 돌연변이;
YALI0_C11165g 유전자에서 1523T>C 돌연변이;
YALI0_C16247g 유전자에서 T1412A, G1441A, A1513G, A1534G, G1535T, T1544A 및 C1847T 돌연변이;
YALI0_D24849g 유전자에서 103G>A 돌연변이;
YALI0_D27016g 유전자에서 446T>C 돌연변이;
YALI0_E14388g1 유전자에서 2525C>G, 2531T>G, 2534A>G 및 2558T>C 돌연변이;
YALI0_E23969g 유전자에서 480_481insTCCTCTACCCCCGAG, 526_527insCCTCCACCACCGAGC 및 497A>C 돌연변이;
YALIO_F04906g 유전자에서 2702T>A;
YALI0_F17468g 유전자에서 482_483insGCACCA 돌연변이;
YALI0_A00891g 유전자에서 11_16delACGGCC 돌연변이;
YALI0_A00935g 유전자에서 202_203insGCTC 돌연변이;
YALI0_A02002g 유전자에서 1255_1256insGAGGTCAAGGTC 돌연변이;
YALI0_A02497g 유전자에서 132_137dupCAACTC 및 331_332insCCCACT 돌연변이;
YALI0_A07997g 유전자에서 2846_2847insGGAGCAGGA 및 2869_2870insAGGAGGAGG 돌연변이;
YALI0_A13849g 유전자에서 165_166insCAAA 돌연변이;
YALI0_A16863g 유전자에서 888_889insGAGCTGAGATGAC 돌연변이;
YALI0_A17578g 유전자에서 432_433insGCGGAGCCG 돌연변이;
YALI0_A17776g 유전자에서 959_960insACAGCAGAT 돌연변이;
YALI0_A17853g 유전자에서 1940_1942delAGG, 1990_1991insAGGAGGAGGCTAAGAAGA 및 2817_2818insTCTGAG 돌연변이;
YALI0_A19646g 유전자에서 1462_1463insGGG 돌연변이;
YALI0_B00748g 유전자에서 525_526insCCCGAC 돌연변이;
YALI0_B08800g 유전자에서 152_153delGT 돌연변이;
YALI0_C06424g 유전자에서 1370_1373delACTT 돌연변이;
YALI0_C07172g 유전자에서 308_309insGCAGCGACA 돌연변이;
YALI0_C08437g 유전자에서 1592_1593insGACAGTCAGCAC 돌연변이;
YALI0_C09031g 유전자에서 1789_1790insCTCCCGAGTCCTCTGCTGAGCCTACCAGCGAAGAGACTTCTTCCG 돌연변이;
YALI0_C09614g 유전자에서 1620_1622delACA 돌연변이;
YALI0_C13728g 유전자에서 99_100insAAAAAGTGGTCGAAAAAGTGGCCA 및 129_130insTGGCCGAAAAAGTGGCCAAAA 돌연변이;
YALI0_C14476g 유전자에서 778_779insTGC 돌연변이;
YALI0_C15532g 유전자에서 1611_1616delCAGCTT 돌연변이;
YALI0_C16148g 유전자에서 961_969delAGCAGCAGT 돌연변이;
YALI0_D15752g 유전자에서 1038_1039insCAG 돌연변이;
YALI0_D17820g 유전자에서 619_620insCCCACCCGCAAACCC 돌연변이;
YALI0_D18381g 유전자에서 114_115insCCTCTCACCAACTCA 돌연변이;
YALI0_D19822g 유전자에서 310_315delAAAGAG 돌연변이;
YALI0_D20064g 유전자에서 67_68insGGGGGGGG 돌연변이;
YALI0_D20526g 유전자에서 354_355insTCCACCGGA 돌연변이;
YALI0_D20790g 유전자에서 37_38insCACGTGAAAGTAGCCGAA 돌연변이;
YALI0_D24563g 유전자에서 84_85insGCT 돌연변이;
YALI0_D25014g 유전자에서 484_485insCCGCTAGCGCCAACTCTGGCTCGGAGC 돌연변이;
YALI0_D25058g 유전자에서 592_594dupAAG 돌연변이;
YALI0_D26257g 유전자에서 1478_1480delAGA돌연변이;
YALI0_D26510g 유전자에서 1287_1288insAAG 돌연변이;
YALI0_D26620g 유전자에서 926_927insCGATGAGGACGA 돌연변이;
YALI0_E07832g 유전자에서 499_500insCCAAGCCCCCCGCTTCCAAGCCCACCGCTT 돌연변이;
YALI0_E08008g 유전자에서 794_795insCTCTTCCTCTTCCTCTTCCTCTTCCTCTTC 돌연변이;
YALI0_E11363g 유전자에서 74_77delCACA 돌연변이;
YALI0_E13596g 유전자에서 1053_1054insCAACAACAACAGCAACAA 돌연변이;
YALI0_E16731g 유전자에서 1622_1623insTGAGGAGGAAGAGTAGGATGAGGAGTA 돌연변이;
YALI0_E18073g 유전자에서 266_267insCCCCACGCAGCAGTCTTG 돌연변이;
YALI0_E18216g 유전자에서 930_938delACAACAGCA 돌연변이;
YALI0_E20449g 유전자에서 899_900insAAACGC 돌연변이;
YALI0_E21109g 유전자에서 228_229insGCCCCGCCT 돌연변이;
YALI0_F12221g 유전자에서 1825_1827delAAG 돌연변이;
YALI0_F12793g 유전자에서 1855_1860delTCTTCT 돌연변이;
YALI0_F16577g 유전자에서 807_808insCCTCCT 돌연변이;
YALI0_F19030g 유전자에서 1344_1345insCCTACTACCGCCGATGTT, 2065T>A, 2098A>G 및 2099C>A 돌연변이; 또는
YALI0_F23287g 유전자에서 1919_1920insCTC 돌연변이;
를 포함하고, D-자일룰로스로부터 D-자일룰로스-5-인산염을 생산하는 효소를 코딩하는 유전자를 포함할 수 있다. 또는, 본 발명의 형질전환된 적응진화 효모 균주는 D-자일로스와 D-자일룰로스를 상호 변환하는 효소를 코딩하는 유전자 및 D-자일룰로스로부터 D-자일룰로스-5-인산염을 생산하는 효소를 코딩하는 유전자를 포함할 수 있다. 상기 D-자일룰로스로부터 D-자일룰로스-5-인산염을 생산하는 효소는 자일룰로스 인산화효소(xylulokinase)일 수 있다. 상기 D-자일룰로스로부터 D-자일룰로스-5-인산염을 생산하는 효소를 코딩하는 유전자는 야로위아 리폴리티카(Yarrowia lipolytica)로부터 유래한 것일 수 있고, 구체적으로 XK(xylulokinase) 유전자일 수 있으며, 보다 구체적으로 서열번호 2의 염기서열로 이루어진 유전자일 수 있다.
본 발명의 적응진화 효모 균주 또는 형질전환된 적응진화 효모 균주는 자일로스(xylose)를 유일 탄소원으로 이용할 수 있다. 또는, 본 발명의 형질전환된 적응진화 효모 균주는 상기 적응진화 효모 균주가 D-자일로스와 D-자일룰로스를 상호 변환하는 효소를 코딩하는 유전자 및 D-자일룰로스로부터 D-자일룰로스-5-인산염을 생산하는 효소를 코딩하는 유전자로 이루어진 군으로부터 선택된 하나 이상의 유전자를 포함하도록 형질전환되어 자일로스를 유일 탄소원으로 이용할 수 있다.
본 발명의 일 실시예에 따르면, 자일로스가 유일 탄소원으로 존재하는 배지에서 5회 계대배양하여 적응진화된 균주로서, 야생형 효모 균주와 비교할 때 적응진화 효모 균주에, 자일로스 이성화효소 유전자만을 도입하여 형질전환된 균주(YSX_xylA)는 대조군(적응진화되지 않은 야생형 효모 균주, OD600이 약 0.88, 균주 성장률(μ)이 약 0.0158 h- 1)에 비해 성장률이 약 1.02배 증가하고, 자일룰로스 인산화효소 유전자만을 도입하여 형질전환된 균주(YSX_XK)는 성장률이 약 1.49배 증가하며, 상기 자일로스 이성화효소 유전자 및 자일룰로스 인산화효소 유전자 모두를 도입하여 형질전환된 균주(YSX_xylA_XK)는 성장률이 약 12배 증가하여, 자일로스를 유일 탄소원으로 이용할 수 있음을 확인하였다(실시예 3).
본 발명의 형질전환된 적응진화 효모 균주는 제 1 벡터 및 상기 제 2 벡터로 이루어진 군으로부터 선택된 하나 이상의 벡터로 형질전환된 것일 수 있다. 상기 "제 1 또는 제 2"라는 표현은, 벡터의 종류를 구별하기 위한 것일 뿐, 형질전환의 순서나 방법을 제한하는 것은 아니다.
상기 제 1 벡터는 D-자일로스와 D-자일룰로스를 상호 변환하는 효소를 코딩하는 유전자를 포함하는 것일 수 있고, 구체적으로 UAS1B 인헨서, TEF(Translational elongation factor) 프로모터, 및 D-자일로스와 D-자일룰로스를 상호 변환하는 효소를 코딩하는 유전자를 포함하는 것일 수 있으며, 보다 구체적으로 서열번호 3의 염기서열로 이루어지는 것일 수 있다.
상기 제 2 벡터는 D-자일룰로스로부터 D-자일룰로스-5-인산염을 생산하는 효소를 코딩하는 유전자를 포함하는 것일 수 있고, 구체적으로 UAS1B 인헨서, TEF(Translational elongation factor) 프로모터, 및 D-자일룰로스로부터 D-자일룰로스-5-인산염을 생산하는 효소를 코딩하는 유전자를 포함하는 것일 수 있으며, 보다 구체적으로 서열번호 4의 염기서열로 이루어지는 것일 수 있다.
본 발명의 형질전환된 적응진화 효모 균주는 기탁번호가 KCTC13616BP인 균주일 수 있다. 상기 기탁번호가 KCTC13616BP인 균주는, 자일로스가 유일 탄소원으로 존재하는 배지에서 5회 계대배양하여 적응진화된, 예를 들어 야생형 효모 균주와 비교할 때 적응진화 효모 균주가 D-자일로스(D-xylose)와 D-자일룰로스(D-xylulose)를 상호 변환하는 효소를 코딩하는 유전자를 포함하는 제 1 벡터로 형질전환된 균주일 수 있고, 또는 상기 적응진화 효모 균주가 D-자일룰로스(D-xylulose)로부터 D-자일룰로스-5-인산염(D-xylulose-5-phosphate)을 생산하는 효소를 코딩하는 유전자를 포함하는 제 2 벡터로 형질전환된 균주일 수 있고, 또는, 상기 적응진화 효모 균주가 상기 제 1 벡터 및 제 2 벡터로 형질전환된 균주일 수 있다.
다른 측면에서, 본 발명은 상기 형질전환된 적응진화 효모 균주 제조방법으로, 야생형 효모 균주를 유일 탄소원으로 자일로스를 포함하는 배지에서 3회 이상 계대배양하는 단계; 및 상기 계대배양된 균주가 D-자일로스(D-xylose)와 D-자일룰로스(D-xylulose)를 상호 변환하는 효소를 코딩하는 유전자 및 D-자일룰로스로(D-xylulose)부터 D-자일룰로스-5-인산염(D-xylulose-5-phosphate)을 생산하는 효소를 코딩하는 유전자로 이루어진 군으로부터 선택된 하나 이상의 유전자를 포함하도록 형질전환하는 단계를 포함할 수 있다. 또는, 상기 형질전환된 적응진화 효모 균주 제조방법으로, 야생형 효모 균주를 유일 탄소원으로 자일로스를 포함하는 배지에서 3회 이상 계대배양하는 단계; 및 상기 계대배양된 균주가 D-자일로스(D-xylose)와 D-자일룰로스(D-xylulose)를 상호 변환하는 효소를 코딩하는 유전자 및 D-자일룰로스로(D-xylulose)부터 D-자일룰로스-5-인산염(D-xylulose-5-phosphate)을 생산하는 효소를 코딩하는 유전자로 이루어진 군으로부터 선택된 하나 이상의 유전자를 포함하도록 형질전환하는 단계를 포함할 수 있다.
상기 계대배양 단계는 야생형 효모 균주를 유일 탄소원으로 자일로스를 포함하는 배지에서 3회 이상, 구체적으로 3회 이상, 4회 이상 또는 5회 이상 계대배양하는 것일 수 있다. 상기 계대배양된 균주는 야생형 효모 균주와 비교할 때 하기 표 2의 유전자로 이루어진 군으로부터 선택된 하나 이상의 유전자에서 하기 표 2와 같은 돌연변이, 또는 하기 표 2의 유전자로 이루어진 군으로부터 선택된 하나 이상의 유전자에서 하기 표 2와 같은 돌연변이 및 하기 표 3의 유전자로 이루어진 군으로부터 선택된 하나 이상의 유전자에서 하기 표 3과 같은 돌연변이를 포함하는 적응진화 균주일 수 있고, 기탁번호가 KCTC13615BP인 적응진화 균주일 수 있다.
유전자 No. 유전자명 돌연변이
7 YALI0_A15642g 147G>A ; p.Trp49*
8 YALI0_A15796g 567_568insACA ; p.Thr189dup, 576T>G ; p.Asp192Glu
21 YALI0_C11165g 1523T>C ; p.Val508Ala
26 YALI0_C16247g T1412A ; Val471Asp, G1441A ; Asp481Asn, A1513G ; Asn505Asp, A1534G ; Ser512Gly, G1535T ; Ser512Ile, T1544A ; Val515Glu,
C1847T ; Ala616Val
35 YALI0_D24849g 103G>A ; p.Ala35Thr
41 YALI0_D27016g 446T>C ; p.Leu149Pro
46 YALI0_E14388g1 2531T>G ; p.Leu844Arg, 2534A>G ; p.Gln845Arg, 2558T>C ; p.Val853Ala
52 YALI0_E23969g 480_481insTCCTCTACCCCCGAG ; p.Ser160_Ser161insSerSerThrProGlu, 526_527insCCTCCACCACCGAGC ; p.Ser176_Ser177insSerThrThrGluPro,
497A>C ; p.Tyr166Ser
53 YALIO_F04906g 2702T>A; p.Val901Glu
57 YALI0_F17468g 482_483insGCACCA ; p.Gln160_His161insGlnHis
del: 결실(deletion), ins: 삽입(insertion), fs: 프레임 시프트(frame shift),
dup: 중복(duplication), *: stop
유전자 No. 유전자명 돌연변이
1 YALI0_A00891g 11_16delACGGCC ; p.Asn4_Gln6delinsLys
2 YALI0_A00935g 202_203insGCTC ; p.Tyr68fs
3 YALI0_A02002g 1255_1256insGAGGTCAAGGTC ; p.Gly418_Gln419insArgGlyGlnGly
4 YALI0_A02497g 132_137dupCAACTC ; p.Ser46_Gly47insAsnSer,331_332insCCCACT ; p.Asn110_Tyr111insSerHis
5 YALI0_A07997g 2846_2847insGGAGCAGGA ; p.Gly949_Glu950insGluGlnGlu,2869_2870insAGGAGGAGG ; p.Lys957_Glu958insGluGluGlu
6 YALI0_A13849g 165_166insCAAA ; p.Lys56fs
9 YALI0_A16863g 888_889insGAGCTGAGATGAC ; p.Val298fs
10 YALI0_A17578g 432_433insGCGGAGCCG ; p.Pro144_Ser145insAlaGluPro
11 YALI0_A17776g 959_960insACAGCAGAT ; p.Pro320_Gln321insGlnGlnIle
12 YALI0_A17853g 1940_1942delAGG ; p.Glu648del, 1990_1991insAGGAGGAGGCTAAGAAGA ;
p.Glu664_Glu665insGluGluAlaLysLysLys,
2817_2818insTCTGAG ; p.Asp939_Ser940insSerGlu
13 YALI0_A19646g 1462_1463insGGG ; p.Glu487_Glu488insGly
14 YALI0_B00748g 525_526insCCCGAC ; p.Asp175_Ser176insProAsp
15 YALI0_B08800g 152_153delGT ; p.Cys51fs
16 YALI0_C06424g 1370_1373delACTT ; p.Tyr457fs
17 YALI0_C07172g 308_309insGCAGCGACA ; p.Gln103_Gln104insGlnArgGln
18 YALI0_C08437g 1592_1593insGACAGTCAGCAC ; p.Lys531_Thr532insThrValSerThr
19 YALI0_C09031g 1789_1790insCTCCCGAGTCCTCTGCTGAGCCTACCAGCGAAGAGACTTCTTCCG ;p.Ser596_Val597insAlaProGluSerSerAlaGluProThrSerGluGluThrSerSer
20 YALI0_C09614g 1620_1622delACA ; p.Gln541del
22 YALI0_C13728g 99_100insAAAAAGTGGTCGAAAAAGTGGCCA ; p.Pro33_Lys34insLysLysTrpSerLysLysTrpPro, 129_130insTGGCCGAAAAAGTGGCCAAAA ; p.Lys43_Lys44insTrpProLysLysTrpProLys
23 YALI0_C14476g 778_779insTGC ; p.Lys260delinsMetGln
24 YALI0_C15532g 1611_1616delCAGCTT ; p.Ser538_Phe539del
25 YALI0_C16148g 961_969delAGCAGCAGT ; p.Ser321_Ser323del
27 YALI0_D15752g 1038_1039insCAG ; Pro346_Gln347insGln
28 YALI0_D17820g 619_620insCCCACCCGCAAACCC ; Thr206_His207insProHisProGlnThr
29 YALI0_D18381g 114_115insCCTCTCACCAACTCA ; Ser38_Thr39insProLeuThrAsnSer
30 YALI0_D19822g 310_315delAAAGAG ; Lys104_Glu105del
31 YALI0_D20064g 67_68insGGGGGGGG ; p.Tyr23fs
32 YALI0_D20526g 354_355insTCCACCGGA ; p.Gly118_Ser119insSerThrGly
33 YALI0_D20790g 37_38insCACGTGAAAGTAGCCGAA ; p.Ser13_Arg14insArgGluSerSerArgThr
34 YALI0_D24563g 84_85insGCT ; p.Glu28_Lys29insAla
36 YALI0_D25014g 484_485insCCGCTAGCGCCAACTCTGGCTCGGAGC ; p.Ala162_Ala163insAlaSerAlaAsnSerGlySerGluPro
37 YALI0_D25058g 592_594dupAAG ; p.Lys198dup
38 YALI0_D26257g 1478_1480delAGA ; p.Glu493_Thr494delinsAla ; 1480/1974 ; 1478/1974 ; 493/657 ;
39 YALI0_D26510g 1287_1288insAAG ; p.Gln429_Gln430insLys
40 YALI0_D26620g 926_927insCGATGAGGACGA ; p.Asp308_Glu309insAspAspGluAsp
42 YALI0_E07832g 499_500insCCAAGCCCCCCGCTTCCAAGCCCACCGCTT ;p.Pro167_Lys168insLysProProAlaSerLysProThrAlaSer
43 YALI0_E08008g 794_795insCTCTTCCTCTTCCTCTTCCTCTTCCTCTTC ; p.Gly265_Ser266insSerSerSerSerSerSerSerSerSerSer
44 YALI0_E11363g 74_77delCACA ; p.Thr25fs
45 YALI0_E13596g 1053_1054insCAACAACAACAGCAACAA ; p.Gln346_Gln351dup
47 YALI0_E16731g 1622_1623insTGAGGAGGAAGAGTAGGATGAGGAGTA ;p.Glu541delinsAspGluGluGluGluTerAspGluGluTer
48 YALI0_E18073g 266_267insCCCCACGCAGCAGTCTTG ; p.Cys89_Pro90insProThrGlnGlnSerCys
49 YALI0_E18216g 930_938delACAACAGCA ; p.Gln311_Gln313del
50 YALI0_E20449g 899_900insAAACGC ; p.Ala300_Asn301insAsnAla
51 YALI0_E21109g 228_229insGCCCCGCCT ; p.Gln76_Ala77insAlaProPro
54 YALI0_F12221g 1825_1827delAAG ; p.Lys609del
55 YALI0_F12793g 1855_1860delTCTTCT ; p.Ser619_Ser620del
56 YALI0_F16577g 807_808insCCTCCT ; p.Leu269_Thr270insProPro
57 YALI0_F19030g 1344_1345insCCTACTACCGCCGATGTT ;p.Val448_Pro449insProThrThrAlaAspVal, 2065T>A ; p.Ser689Thr,
2098A>G ; p.Thr700Ala, 2099C>A ; p.Thr700Asn
58 YALI0_F23287g 1919_1920insCTC ; p.Ser640dup
del: 결실(deletion), ins: 삽입(insertion), fs: 프레임 시프트(frame shift),
dup: 중복(duplication), *: stop
상기 형질전환하는 단계는 상기 계대배양된 균주를 D-자일로스(D-xylose)와 D-자일룰로스(D-xylulose)를 상호 변환하는 효소를 코딩하는 유전자 및 D-자일룰로스로(D-xylulose)부터 D-자일룰로스-5-인산염(D-xylulose-5-phosphate)을 생산하는 효소를 코딩하는 유전자로 이루어진 군으로부터 선택된 하나 이상의 유전자를 포함하도록 형질전환하는 것일 수 있고, 구체적으로 상기 제 1 벡터 및 제 2 벡터로 이루어진 군으로부터 선택된 하나 이상의 벡터로 형질전환하는 단계일 수 있다. 상기 D-자일로스(D-xylose)와 D-자일룰로스(D-xylulose)를 상호 변환하는 효소를 코딩하는 유전자, D-자일룰로스로(D-xylulose)부터 D-자일룰로스-5-인산염(D-xylulose-5-phosphate)을 생산하는 효소를 코딩하는 유전자, 제 1 벡터 및 제 2 벡터에 관한 설명은 전술한 바와 같다.
또 다른 측면에서, 본 발명은 상기 형질전환된 적응진화 효모 균주를 탄소원으로 자일로스를 포함하는 배지에서 배양하는 단계를 포함하는 지질 생산 방법을 제공한다.
상기 형질전환된 적응진화 효모 균주는 자일로스가 유일 탄소원으로 존재하는 배지에서 3회 이상, 구체적으로 3회 이상, 4회 이상 또는 5회 이상 계대배양된 균주일 수 있고, 상기 계대배양된 효모 균주는 야생형 효모 균주와 비교할 때 상기 표 2의 유전자로 이루어진 군으로부터 선택된 하나 이상의 유전자에서 상기 표 2와 같은 돌연변이, 또는 상기 표 2의 유전자로 이루어진 군으로부터 선택된 하나 이상의 유전자에서 상기 표 2와 같은 돌연변이 및 상기 표 3의 유전자로 이루어진 군으로부터 선택된 하나 이상의 유전자에서 상기 표 3과 같은 돌연변이를 포함하는 적응진화 균주일 수 있고, 기탁번호가 KCTC13615BP인 적응진화 균주일 수 있다. 상기 적응진화 균주, 계대배양, 돌연변이 균주, 형질전환된 적응진화 효모 균주 등에 관한 설명은 전술한 바와 같다.
상기 "지질"은 기본적으로 지방산과 글리세롤로 구성된 유기물 또는 유기화합물로서, 아실글리세롤, 글리세라이드 및 자유지방산으로 이루어진 군으로부터 선택된 하나 이상을 포함하는 것일 수 있다. 상기 아실글리세롤은 트리아실글리세롤(Triacylglycerol, TAG), 디아실글리세롤(Diacylglycerol, DAG), 및 모노아실글리세롤(Monoacylglycerol, MAG)로 이루어진 군으로부터 선택된 하나 이상일 수 있다. 상기 글리세라이드는 모노글리세라이드(Monoglyceride), 디글리세라이드(Diglyceride), 및 트리글리세라이드(Triglyceride)로 이루어진 군으로부터 선택된 하나 이상일 수 있다. 또는, 상기 지질은 부티르산(부탄산, C4:0), 카프로산(헥산산, C6:0), 카프릴산(옥탄산, C8:0), 카프르산(데칸산, C10:0), 라우르산(도데칸산, C12:0), 미리스트산(테트라데칸산, C14:0), 미리스톨레산(ω-5, C14:1), 펜타데실릭산(Pentadecylic acid, C15:0), 팔미트산(헥사데칸산, C16:0), 팔미톨레산(ω-7, C16:1), 헥사데카디에노산(Hexadecadienoic acid, C16:2), 헥사데카트리엔산(Hexadecatrienoic acid, C16:3), 마가릭 산(Margaric acid, C17:0), 헵타데노익산(Heptadenoic acid, C17:1), 스테아르산(옥타데칸산, C18:0), 올레산(ω-9, C18:1), 리놀레산(LA, ω-6, C18:2), 알파-리놀렌산(ALA, ω-3, C18:3), 옥타데카테트라에노익산(Octadecatetraenoic acid, C18:4), 노나데실릭산(Nonadecylic acid, C19:0), 노나데실릭산(Nonadecylic acid, C19:1), 아라키드산(에이코산산, C20:0), 아라키돈산(AA, ω-6, C20:4), 에이코사펜타엔산(EPA, ω-3, C20:5), 베헨산(도코산산, C22:0), 에루크산(ω-9, C22:1), 도코사펜타엔산(DPA, ω-3, 22:5), 및 도코사헥사엔산(DHA, ω-3, C22:6)으로 이루어진 군으로부터 선택된 하나 이상일 수 있으며, 구체적으로 C16:0, C16:1, C16:2, C16:3, C18:0, C18:1, C18:2, C18:3, 및 C18:4으로 이루어진 군으로부터 선택된 하나 이상의 지방산일 수 있다.
상기 배양에 사용되는 배지는 배양하고자 하는 미생물의 특징에 따라 적절하게 선택하여 사용할 수 있으며, 배양하고자 하는 미생물이 효모 균주, 구체적으로 야로위아 리폴리티카 균주일 경우, YSC(Yeast Synthetic Complete)배지를 사용할 수 있다. 또한, 상기 배양에 사용되는 배지는 적당한 탄소원, 질소원, 아미노산, 비타민 등으로 이루어진 군에서 선택된 하나 이상을 함유한 통상의 배지 내에서 온도, pH 등을 조절하면서 적절한 방식으로 효모 균주의 배양 요건을 충족하는 것을 사용할 수 있다. 예를 들면, 사용될 수 있는 탄소원으로는 글루코즈, 자일로즈, 수크로즈, 락토즈, 프락토즈, 말토즈, 전분, 셀룰로즈와 같은 당 및 탄수화물, 대두유, 해바라기유, 피마자유, 코코넛유 등과 같은 오일 및 지방, 팔미트산, 스테아린산, 리놀레산과 같은 지방산, 글리세롤, 에탄올과 같은 알코올, 아세트산과 같은 유기산, 아세트산, 부티르산, 이소부티르산, 프로피온산, 발레르산, 이소발레르산, 카프로산 등과 같은 휘발성 지방산(volatile fatty acid, VFA) 등이 포함될 수 있다. 이들 물질은 개별적으로 또는 혼합되어 포함될 수 있다. 사용될 수 있는 질소원으로는 암모니아, 황산암모늄, 염화암모늄, 초산암모늄, 인산암모늄, 탄산안모늄, 및 질산암모늄과 같은 무기질소원; 글루탐산, 메티오닌, 글루타민과 같은 아미노산 및 펩톤, NZ-아민, 육류 추출물, 효모 추출물, 맥아 추출물, 옥수수 침지액, 카세인 가수분해물, 어류 또는 그의 분해생성물, 탈지 대두 케이크 또는 그의 분해생성물 등 유기질소원이 사용될 수 있다. 이들 질소원은 단독 또는 조합되어 사용될 수 있다. 상기 배지에는 인원으로서 인산 제1칼륨, 인산 제2칼륨 및 대응되는 소듐-함유 염이 포함될 수 있다. 사용될 수 있는 인원으로는 인산이수소칼륨 또는 인산수소이칼륨 또는 상응하는 나트륨-함유 염이 포함된다. 또한, 무기화합물로는 염화나트륨, 염화칼슘, 염화철, 황산마그네슘, 황산철, 황산망간 및 탄산칼슘 등이 사용될 수 있다. 마지막으로, 상기 물질에 더하여 아미노산 및 비타민과 같은 물질이 사용될 수 있다.
또한, 상기 형질전환된 적응진화 효모 균주의 배양은 자일로스를 탄소원으로 사용하여 배양하는 것일 수 있다.
또한, 배양 배지에 적절한 전구체들이 사용될 수 있다. 상기된 원료들은 배양과정에서 배양물에 적절한 방식에 의해 회분식, 유가식 또는 연속식으로 첨가될 수 있으나, 특별히 이에 제한되지는 않는다. 적절한 농도의 수산화나트륨, 수산화칼륨, 암모니아와 같은 기초 화합물 또는 인산 또는 황산과 같은 산 화합물을 적절한 양과 방식으로 사용하여 배양물의 pH를 조절할 수 있다.
또한, 상기 형질전환된 적응진화 효모 균주의 배양은 진탕배양, 정치배양, 회분식 배양, 유가식 배양, 및 연속식 배양으로 이루어진 군에서 선택되는 어느 하나에 의한 배양일 수 있다. 상기 진탕배양은 미생물을 접종한 배양물을 흔들면서 배양하는 방법을, 상기 정치배양은 미생물을 접종한 액체 배양물을 흔들지 아니하고 놓아둔 채로 배양하는 방법을 의미하고, 상기 회분식 배양은 배양물의 부피를 고정하고 외부에서 새로이 배양물을 첨가하지 아니한 상태에서 배양하는 방법을 의미하고 이러한 배양 방법을 실현할 수 있는 장치를 회분식 반응기라 한며, 상기 유가식 배양은 원료를 최초에 전부 배양 탱크에 넣어서 배양하는 단일배양(batch)에 대조되는 말로서 먼저 소량의 원소를 넣고 이것에 소량씩 원료를 추가하여 가며 배양하는 방법을 의미하고 이러한 배양 방법을 실현할 수 있는 장치를 유가식 반응기라고 하며, 상기 연속식 배양은 새로운 영양배지가 계속 공급되며 동시에 세포 및 생산물을 포함하는 배양물이 계속 제거되는 배양방법을 의미하고, 이러한 배양 방법을 실현할 수 있는 장치를 연속식 반응기라고 한다. 구체적으로 상기 배양은 회분식 반응기, 연속식 반응기, 또는 유가식 반응기에서 실시하는 것일 수 있다. 본 발명의 일 실시예에 따르면, 회분식 반응기를 운전하여 효모 균주, 구체적으로 형질전환된 적응진화 효모 균주를 배양할 경우 고농도로 효모 균주를 배양할 수 있으며, 그러한 배양물을 이용하여 고농도의 지질을 생산할 수 있다.
또한, 상기 형질전환된 적응진화 효모 균주의 배양은 OD600 은 1.0 이상, 1.5 이상, 이상, 2.0 이상, 2.2 이상, 2.4 이상, 2.6 이상, 2.8 이상, 3.0 이상, 3.2 이상, 3.4 이상, 3.6 이상, 3.8 이상, 4.0 이상, 4.2 이상, 4.4 이상, 4.6 이상, 4.8 이상, 5.0 이상, 5.2 이상, 5.4 이상, 5.6 이상, 5.8 이상, 6.0 이상, 6.2 이상, 6.4 이상, 6.6 이상, 6.8 이상, 7.0 이상, 7.2 이상, 7.4 이상, 7.6 이상, 7.8 이상, 8.0 이상, 8.2 이상, 8.4 이상, 8.6 이상, 8.8 이상, 9.0 이상, 9.2 이상, 9.4 이상, 9.6 이상, 9.8 이상, 10.0 이상, 10.2 이상, 10.4 이상, 10.6 이상, 10.8 이상, 11.0 이상, 11.2 이상, 11.4 이상, 11.6 이상, 11.8 이상, 12.0 이상, 12.2 이상, 12.4 이상, 12.6 이상, 12.8 이상, 13.0 이상, 13.2 이상, 13.4 이상, 13.5 이상, 13.6 이상, 13.8 이상, 14.0 이상, 20 이상, 30 이상, 40 이상, 42 이상, 44 이상일 수 있고, 상기 OD600은 120 이하, 115 이하, 110 이하, 105 이하, 100 이하, 95 이하, 90 이하, 85 이하, 80 이하, 70 이하, 60 이하, 50 이하, 48 이하, 46 이하, 45 이하, 44 이하, 42 이하, 40 이하, 30 이하, 20 이하, 18 이하, 16 이하, 15 이하, 14 이하, 13.8 이하, 13.6 이하, 13.5 이하, 13.4 이하, 13.2 이하, 13.0 이하, 12 이하, 10 이하, 5 이하, 2 이하 또는 1 이하일 수 있다.
또 다른 측면에서, 본 발명은 상기 형질전환된 효모 적응진화 균주를 탄소원으로 자일로스를 포함하는 배지에서 배양하여 지질을 생산하는 단계; 및 상기 생산된 지질을 트랜스에스테르화하여 바이오디젤을 수득하는 단계;를 포함하는, 바이오디젤 생산 방법을 제공한다. 상기 적응진화 균주, 형질전환된 적응진화 효모 균주, 상기 균주의 배양, 지질 생산 등에 관한 설명은 전술한 바와 같다.
상기 "바이오디젤"은 바이오연료 중 하나로서, 보통 메탄올을 이용해 3가의 지방산에 글리세롤이 결합한 트라이글리세리드로부터 글리세롤을 분리한 다음, 지방산에스터를 만들어 내는 에스테르 교환방법을 통하여 만든 지방산 메틸에스테르(fatty acid methyl ester, FAME)를 말한다. 상기 생산된 지질을 트랜스에스테르화하여 바이오디젤을 수득하는 것은 지질로부터 바이오디젤을 수득하는 방법이라면 제한되지 않는다.
이하, 본 발명을 하기의 실시예를 통하여 설명한다. 하기 실시예는 본 발명에 대한 이해를 돕기 위해 예시의 목적으로만 제공된 것일 뿐 본 발명의 범주 및 범위가 그에 의해 제한되는 것은 아니다.
[ 실시예 1] 자일로스 이성화효소 유전자 및 자일룰로스 인산화효소 유전자를 발현하는 균주의 제작
자일로스 이용능을 갖는 야로위아 리폴리티카 균주를 개발하기 위하여 자일로스 이성화효소(xylose isomerase)를 코딩하는 유전자 또는 자일룰로스 인산화효소(xylulokinase)를 코딩하는 유전자를 포함하는 야로위아 리폴리티카(Yarrowia lipolytica) 균주를 제작하고, 자일로스를 이용한 야로위아 리폴리티카 균주의 성장 곡선을 바탕으로 자일로스 이용능을 비교하였다.
구체적으로, 자일로스 이성화효소를 코딩하는 유전자로 xylA3* 유전자(피로마이세스 종(Piromyces sp .)에서 기원한 xylA (xylose isomerase) 유전자가 성능이 향상되도록 개량된 돌연변이 유전자임, 이하에서는 xylA로 표기함), 및 자일룰로스 인산화효소를 코딩하는 유전자로 야로위아 리폴리티카(Yarrowia lipolytica)에서 기원한 XK(xylulokinase) 유전자를 선택하고, 자일로스 이성화효소 및 자일룰로스 인산화효소를 갖고 있지 않은 야로위아 리폴리티카 야생형 균주에 상기 xylA 유전자 또는 XK 유전자를 하기의 방법으로 도입하였다. 먼저, 상기 두 유전자를 과발현시키기 위해, 본 기술분야에서 알려진 벡터 제조방법을 사용하여, 제 1 벡터로 전단에 인헨서(Enhancer, UAS1B)가 더해진 TEF (Translational elongation factor) 프로모터에 서열번호 1의 서열로 이루어지는 xylA 유전자를 삽입하여 pMCS-UAS1B16-TEF-XylA-CYCt (서열번호 3)을 제작하고, 제 2 벡터로 전단에 인헨서(UAS1B)가 더해진 TEF 프로모터에 서열번호 2의 서열로 이루어지는 XK 유전자를 삽입하여 pMCS-UAS1B12-TEF-XK-CYCt (서열번호 4)을 각각 제작하였다. 그런 다음, 상기 3 종의 균주로, 야로위아 리폴리티카 야생형 균주에 제 1 벡터만을 삽입하여 형질전환된 균주 (도 1의 XYLA(O), XK(X) 균주), 야로위아 리폴리티카 야생형 균주에 제 2 벡터만을 삽입하여 형질전환된 균주 (도 1의 XYLA(X), XK(O) 균주) 및 야로위아 리폴리티카 야생형 균주에 제 1 벡터 및 제 2 벡터 둘 다를 삽입하여 형질전환된 균주 (도 1의 XYLA(O), XK(O) 균주)를 제작하였다. 이 때 야로위아 리폴리티카 야생형 균주에 xylA 유전자 및 XK 유전자 모두가 삽입되지 않은 공벡터(pMCS-UAS1B16-TEF-CYCt 및 pMCS-UAS1B12-TEF-CYCt)를 도입하여 대조군 균주를 확보하였다 (도 1의 XYLA(X), XK(X) 균주).
그런 다음, 상기 4종의 균주들을 자일로스를 탄소원으로 하는 최소배지(YSC medium, Yeast Synthetic Complete medium)에 접종한 후 28℃에서 200rpm로 교반하며 배양함으로써 자일로스를 이용한 균주의 성장을 확인하였으며, 이를 바탕으로 재조합균주의 자일로스 이용능을 평가하고, 그 결과를 도 1에 나타내었다. 본 명세서에서 "OD600"는 파장 600nm의 빛에서의 흡광도나 광학밀도(Optical density)를 뜻하는 것으로서, 분광광도계(spectrophotometer) 등과 같은 장비를 통해 측정할 수 있고, 측정된 OD600을 통해 용액이나 시료 속의 미생물이나 세포의 밀도나 농도를 확인할 수 있다.
도 1에 나타난 바와 같이 4종의 균주는 28 내지 60 시간 내에 균주 성장 정체기(stationary phase)에 도달하였고 자일로스 이성화효소 유전자만을 발현하는 균주(도 1의 XYLA(O), XK(X) 균주)의 경우 배양한지 60 시간 후에 성장 정체기에서의 OD600 값이 0.4961로 나타나 자일로스 기반의 균주 성장이 가장 높은, 즉 자일로스 이용능이 가장 높은 균주라는 것을 알 수 있었다. 한편, 자일로스 이성화효소 및 자일룰로스 인산화효소를 모두 발현하는 균주(도 1의 XYLA(O), XK(O) 균주)의 경우 대조군(도 1의 XYLA(X), XK(X) 균주) 대비(OD600=0.3957) 소폭 증가된 성장을 보이며 배양 60 시간 경과 시 OD600 값이 0.4210을 나타냈으나, 자일로스 배지 내 세포 성장에 있어 대조군과 큰 차이를 보이지 않았다. 즉, 자일로스 이성화효소 또는 자일룰로스 인산화효소를 발현하는 모든 실험군이 전체적으로 대조군 대비 세포 성장에 있어 큰 개선을 보이고 있지 않으며 자일로스 이성화효소 및 자일룰로스 인산화효소 유전자를 야생형에 도입하는 것만으로는 자일로스 이용능에 있어 그 효과가 미미함을 확인할 수 있었다.
[ 실시예 2] 진화 공학적 방법을 통한 자일로스 이용능을 갖는 야로위아 리폴리티카 적응진화 균주의 제작
야로위아 리폴리티카 야생형 균주의 자일로스 대사를 개선하기 위해 야생형 균주를 자일로스 최소배지(YSC medium, Yeast Synthetic Complete medium)에서 계대배양하여 자일로스 이용에 적응 진화된 균주를 확보하고자 하였다. 이를 위해 자일로스 이성화효소(xylose isomerase)를 발현하는 야로위아 리폴리티카 야생형 균주를 20g/L의 자일로스가 유일 탄소원으로 존재하는 최소배지(CSM(Complete Supplement Mixture)-Leu-Ura 배지(6.7g/L yeast nitrogen base, 20g/L xylose, CSM-Leu-Ura(MP biomedicals, Solon, USA))에서 계대배양하였으며, 그 결과 계대배양 5회 이후 자일로스 이용능이 향상된 적응진화 균주(도 2의 YSX 균주)를 분리할 수 있었다. 상기 적응진화 균주(YSX 균주)는 상기 표 1과 같이 유전자에 돌연변이가 발생하였음을 확인하였다.
상기 적응진화된 균주(YSX 균주)의 자일로스 이용능을 야생형 균주와 비교하였을 때, 도 2에 나타난 바와 같이 균주 성장 정체기(stationary phase)에서의 최대 균주 농도가 OD600 기준으로 야생형 균주는 0.50, 적응진화된 균주(YSX 균주)는 1.57로 나타났으며, 적응진화된 균주는 야생형 균주에 비해 세포 성장율이 약 215% 증가하였다. 또한, 성장율(growth rate, 단위:μ)을 기준으로 하였을 때 역시 적응진화된 균주(YSK 균주)는 성장율이 0.006 h-1로, 야생형 균주가 0.002 h-1인 것에 비해 약 3 배 향상된 자일로스 이용능을 나타내는 것을 확인하였다.
이를 통해 적응 진화된 균주는 자일로스 이성화효소를 발현하는 야로위아 리폴리티카 야생형 균주에 비해 자일로스 이용능이 매우 향상되어 자일로스를 유일 탄소원으로 하여 성장할 수 있음을 알 수 있었다.
[ 실시예 3] 적응 진화된 균주에서의 자일로스 이성화효소 유전자 및 자일룰로스 인산화효소 유전자 발현
자일로스 이용능이 증가된 야로위아 리폴리티카 균주를 제조하기 위해, 상기 실시예 2에서 제조한 야로위아 리폴리티카 적응진화 균주(YSX 균주)가 자일로스 대사경로 관련 유전자를 과발현할 수 있도록 형질전환하였다.
구체적으로, 상기 실시예 2에서 제조한 야로위아 리폴리티카 적응진화 균주(YSX 균주)에 상기 실시예 1와 동일한 방법으로 자일로스 이성화효소 유전자(xylA 유전자) 또는 자일룰로스 인산화효소 유전자(XK 유전자)를 삽입하여 형질전환 균주를 제조하였다. 상기 형질전환 균주는 상기 실시예 2에서 제조한 야로위아 리폴리티카 적응진화 균주(YSX 균주)에 상기 실시예 1의 자일로스 이성화효소 유전자(xylA 유전자)만을 도입한 균주 (도 3의 YSX_xylA 균주), 상기 실시예 2의 야로위아 리폴리티카 적응진화 균주(YSX 균주)에 자일룰로스 인산화효소 유전자(XK 유전자)만을 도입한 균주 (도 3의 YSX_XK 균주), 및 상기 실시예 2에서 제조한 야로위아 리폴리티카 적응진화 균주(YSX 균주)에 상기 자일로스 이성화효소 유전자(xylA 유전자) 및 자일룰로스 인산화효소 유전자(XK 유전자) 둘 다를 도입한 균주 (도 3의 YSX_xylA_XK 균주)의 3종이다. 이 때, 실시예 2의 야로위아 리폴리티카 적응진화 균주(YSK 균주)를 대조군으로 하였다. 그런 다음, 상기 3종의 형질전환 균주 및 대조군 균주 각각을 20g/L의 자일로스가 탄소원으로 존재하는 최소배지(CSM(Complete Supplement Mixture) 배지, (6.7g/L yeast nitrogen base, 20g/L xylose, CSM (MP biomedicals, Solon, USA)))에서 배양하며 각 균주의 자일로스 이용능을 상기 실시예 2와 마찬가지 방법으로 자일로스를 이용한 균주의 성장 지표(OD600와 성장율(μ))를 비교함으로서 평가하고, 그 결과를 도 3에 나타내었다.
도 3에 나타난 바와 같이, 대조군 적응진화 균주(YSX 균주)의 경우 균주 성장 정체기의 OD600가 약 0.88, 균주 성장률(μ)이 약 0.0158 h-1였으나, 자일로스 이성화효소 유전자(xylA)가 도입된 균주(YSX_xylA)는 OD600가 0.99, 균주 성장률이 0.0162 h-1로 증가하였다. 자일룰로스 인산화효소 유전자(XK)가 도입된 균주(YSX_XK)는 자일로스 이용능이 더욱 향상되어 OD600가 8.82, 균주 성장률이 0.0235 h-1까지 증가하였으며, 자일로스 이성화효소 유전자(xylA)와 자일룰로스 인산화효소 유전자(XK)가 모두 도입된 균주(YSX_xylA_XK)는 OD600가 13.51, 균주 성장률이 0.0250 h-1까지 증가하였다. 상기 YSX_xylA_XK 균주의 균주 성장 지표는 각각 실시예 1의 대조군인 야생종 대비 (OD600 = 0.3596, 균주 성장률 0.0027 h- 1)최대 균주 농도(OD600)는 약 38 배, 성장률은 약 9 배 이상증가하였다.
따라서, 본 발명에 따른 적응진화된 야로위아 리폴리티카 균주에 자일로스 이성화효소 또는 자일룰로스 인산화효소 유전자를 발현하는, 형질전환된 야로위아 리폴리티카 적응 진화 균주의 자일로스 이용능은 야생형 균주와 비교할 때 현저하게 증가함을 알 수 있었다.
[ 실시예 4] 형질전환된 야로위아 리폴리티카 적응진화 균주의 자일로스 이용능 및 지질 생산능 평가
형질전환된 야로위아 리폴리티카 적응진화 균주의 자일로스를 이용하여 지질 생산하는 능력을 평가하기 위해, 상기 실시예 3에서 제조된 형질전환된 야로위아 리폴리티카 적응 진화 균주(YSX_xylA_XK)의 실제 자일로스 이용능을 평가하기 위하여 회분식 반응기에서 배양하며 자일로스를 이용한 균주 성장과 자일로스 소모 경향을 확인하였다. 회분식 반응기를 이용하여 효모 균주를 배양할 경우 고농도로 효모 균주를 배양할 수 있어 고농도의 지질을 생산할 수 있다.
구체적으로, 상기 실시예 3에서 제조된 형질전환된 야로위아 리폴리티카 적응진화 균주(YSX_xylA_XK)를 회분식 반응기에 넣어서 배양하되, 28℃의 온도에서 용존 산소 농도를 50% 이상으로 유지하기 위해 교반 속도를 250 내지 800rpm로 조정하였으며, 반응기 내 pH가 3.5로 유지되도록 하기 위하여 필요한 경우 2.5M NaOH 용액이 첨가하였다. 이 때, 고농도 지질 생산을 위하여 자일로스 160g/L를 반응기 내에 첨가하였으며 자일로스를 더 소모하지 못하는 시점인, 약 256 시간 후에 반응기 운전을 중지하고, 시간에 따른 균주 성장 및 자일로스 농도를 측정하고 그 결과를 도 4에 나타내었다. 여기서, 자일로스 이성화효소 및 자일룰로스 인산화효소를 갖고 있지 않고, 상기 실시예 2와 같은 적응 진화 과정을 거치지 않은 야로위아 리폴리티카 야생형 균주(상기 실시예 1의 대조군 균주)를 대조군으로 하였다.
도 4에 나타난 바와 같이, 자일로스를 거의 소모하지 못하는 야생형 균주와 달리 상기 실시예 3에서 제조된 형질전화된 야로위아 리폴리티카 적응 진화 균주(YSX_xylA_XK)는 고농도(160g/L)의 자일로스 배지 내에서 균주 최대 성장농도가 OD600 기준으로 44.79까지 증가하였다.
또한, 지질 생산량을 측정한 결과, 도 5에 나타난 바와 같이, 상기 실시예 3에서 제조된 형질전화된 야로위아 리폴리티카 적응 진화 균주(YSX_xylA_XK)는 60g/L의 자일로스로부터 약 4g/L의 지질을 축적하였는바, 지질 생산량은 대조군에 비해 약 11배 이상임을 확인하였다.
따라서, 본 발명에 따른 적응진화된 야로위아 리폴리티카 균주에 자일로스 이성화효소 또는 자일룰로스 인산화효소 유전자를 발현하는, 형질전환된 야로위아 리폴리티카 적응진화 균주는, 야생형 균주에 비해 현저하게 우수한 자일로스 이용능 및 지질 생산능이 있음을 알 수 있었다.
한국생명공학연구원 KCTC13615BP 20180807 한국생명공학연구원 KCTC13616BP 20180807
<110> KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY <120> YEAST STRAIN WITH XYLOSE UTILIZING CAPACITY <130> 18p241/ind <160> 63 <170> KoPatentIn 3.0 <210> 1 <211> 1314 <212> DNA <213> Artificial Sequence <220> <223> xylA <400> 1 atggctaagg agtatttccc tcagattcag aagatcaagt tcgacggcaa ggacagcaaa 60 aaccccctgg ctttccatta ttacgacgcc gagaaggaag tgatgggcaa gaagatgaag 120 gactggctgc ggttcgcaat ggcatggtgg cacaccctgt gcgcagaggg agcagatcag 180 ttcggaggag gcaccaagtc ttttccatgg aacgagggca cagacgccat cgagatcgcc 240 aagcagaagg tggatgccgg cttcgagatc atgcagaagc tgggcatccc ctactattgt 300 tttcacgacg tggatctggt gtccgagggc aactctatcg gcgagtacga gtccaatctg 360 aaggccgtgg tggcctatct gaaggacaag cagaaggaga ccggcatcaa gctgctgtgg 420 agctccgcca acgtgttcgg ccacaagagg tacatgaacg gcgcctctac caatcccgac 480 tttgatgtgg tggcccgcgc catcgtgcag atcaagaacg ccatcgatac aggcatcgag 540 ctgggcgccg agaattacgt gttttggggc ggccgggagg gctatatgtc tctgctgaat 600 acagaccaga agagagagaa ggagcacatg gccaccatgc tgacaatggc cagggattac 660 gcccgcagca agggcttcaa gggcaccttt ctgatcgagc ccaagcctat ggagcctaca 720 aagcaccagt atgacgtgga taccgagaca gccatcggct tcctgaaggc ccacaatctg 780 gacaaggact tcaaggtgaa catcgaagtg aatcacgcca ccctggccgg ccacacattc 840 gagcacgagc tggcatgcgc agtggacgca ggaatgctgg gaagcatcga cgccaacagg 900 ggcgattacc agaatggctg ggacaccgat cagtttccta tcgatcagta tgagctggtg 960 caggcatgga tggagatcat caggggagga ggattcgtga ccggaggaac aaactttgac 1020 gccaagaccc ggagaaatag cacagacctg gaggatatca tcatcgccca cgtgtccgga 1080 atggatgcaa tggcacgggc cctggagaac gcagcaaagc tgctgcagga gtccccatac 1140 accaagatga agaaggagag atatgcctct ttcgacagcg gcatcggcaa ggactttgag 1200 gatggcaagc tgacactgga gcaggtgtac gagtatggca agaagaatgg cgagcctaag 1260 cagacctcag ggaagcagga gctgtacgaa gccatcatcg caatgtatca gtga 1314 <210> 2 <211> 1643 <212> DNA <213> Yarrowia lipolytica <400> 2 atgtatctcg gactggatct ttcgactcaa cagctcaagg gcatcattct ggacacaaaa 60 acgctggaca cggtcacaca agtccatgtg gactttgagg acgacttgcc gcagttcaac 120 accgaaaagg gcgtctttca cagctctaca gtggccggag aaatcaatgc tcctgtggca 180 atgtgggggg cagctgtgga cttgctgata gagcgtctgt caaaggaaat agacctttcc 240 acgatcaagt ttgtgtcggg ctcgtgccag caacacggct ctgtttatct caacagcagc 300 tacaaggagg gcctgggttc tctggacaaa cacaaagact tgtctacagg agtgtcatcc 360 ttactggcgc tcgaagtcag ccccaattgg caggatgcaa gcacggagaa ggagtgtgcg 420 cagtttgagg ctgcagtcgg cggtcccgag cagctggctg agatcactgg ctctcgagca 480 catactcgtt tcaccgggcc ccagattctc aaggtcaagg aacgcaaccc caaggtattc 540 aaggccacgt cacgggtcca gctcatatcc aactttctag catctctgtt tgccggcaag 600 gcgtgcccct ttgatcttgc tgacgcctgt ggaatgaatc tgtgggacat ccagaatggc 660 cagtggtgca agaaactcac agatctcatc accgatgaca cccactcggt cgagtccctc 720 cttggagacg tggaaacaga ccccaaggct ctactgggca aaatctcgcc ctatttcgtc 780 tccaagggct tctctccctc ttgtcaggtg gcacagttca caggcgacaa cccaggcact 840 atgctggctc tccccttaca ggccaatgac gtgattgtgt ctttgggaac atctacgacc 900 gccctcgtcg taacaaacaa gtacatgccc gaccccggat accatgtgtt caaccacccc 960 atggagggat acatgggcat gctgtgctac tgcaacggag gtctagcacg agagaagatc 1020 cgagacgagc ttggaggctg ggacgagttt aatgaggcgg ccgagaccac caacacagtg 1080 tctgctgacg atgtccatgt tggcatctac tttccactac gagaaatcct tcctcgagca 1140 ggtccctttg aacgacgttt catctacaac agacaaagtg aacagcttac agagatggct 1200 tctccagagg actcactggc aaccgaacac aaaccgcagg ctcaaaatct caaggacacg 1260 tggccgccac aaatggacgc cactgccatc attcaaagcc aggccctcag tatcaaaatg 1320 agactccaac gcatgatgca tggcgatatt ggaaaggtgt attttgtggg aggcgcctcg 1380 gtcaacactg ctatctgcag cgtaatgtct gccatcttaa aaccaacaaa gggcgcttgg 1440 agatgtggtc tggaaatggc aaacgcttgt gccattggaa gtgcccatca cgcctggctt 1500 tgcgacccca acaagacagg ccaggtacag gttcacgaag aagaggtcaa atacaagaat 1560 gtggacacag acgtgctact caaggcgttc aagctggccg aaaacgcctg cctggagaaa 1620 taactaacta ccacaccaac aca 1643 <210> 3 <211> 9688 <212> DNA <213> Artificial Sequence <220> <223> pMCS-UAS1B16-TEF-XylA-CYCt <400> 3 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccgctttt 420 cgtagataat ggaatacaaa tggatatcca gagtatacac atggatagta tacactgaca 480 cgacaattct gtatctcttt atgttaacta ctgtgaggcg ttaaatagag cttgatatat 540 aaaatgttac atttcacagt ctgaactttt gcagattacc taatttggta agatattaat 600 tatgaactga aagttgatgg catccctaaa tttgatgaaa gggcgcggga tccggcccgg 660 gaattcgaag gtaccaagga agcatgcggt acccgaattc ctgaggtgtc tcacaagtgc 720 cgtgcagtcc cgcccccact tgcttctctt tgtgtgtagt gtacgtacat tatcgagacc 780 gttgttcccg cccacctcga tccggtctag actgaggtgt ctcacaagtg ccgtgcagtc 840 ccgcccccac ttgcttctct ttgtgtgtag tgtacgtaca ttatcgagac cgttgttccc 900 gcccacctcg atccggggat ccctgaggtg tctcacaagt gccgtgcagt cccgccccca 960 cttgcttctc tttgtgtgta gtgtacgtac attatcgaga ccgttgttcc cgcccacctc 1020 gatccgggtc gacctgaggt gtctcacaag tgccgtgcag tcccgccccc acttgcttct 1080 ctttgtgtgt agtgtacgta cattatcgag accgttgttc ccgcccacct cgatccggga 1140 gctcctgagg tgtctcacaa gtgccgtgca gtcccgcccc cacttgcttc tctttgtgtg 1200 tagtgtacgt acattatcga gaccgttgtt cccgcccacc tcgatccggt ctagactgag 1260 gtgtctcaca agtgccgtgc agtcccgccc ccacttgctt ctctttgtgt gtagtgtacg 1320 tacattatcg agaccgttgt tcccgcccac ctcgatccgg ggatccctga ggtgtctcac 1380 aagtgccgtg cagtcccgcc cccacttgct tctctttgtg tgtagtgtac gtacattatc 1440 gagaccgttg ttcccgccca cctcgatccg ggtcgacctg aggtgtctca caagtgccgt 1500 gcagtcccgc ccccacttgc ttctctttgt gtgtagtgta cgtacattat cgagaccgtt 1560 gttcccgccc acctcgatcc gggcatgcgg tacccgaatt cctgaggtgt ctcacaagtg 1620 ccgtgcagtc ccgcccccac ttgcttctct ttgtgtgtag tgtacgtaca ttatcgagac 1680 cgttgttccc gcccacctcg atccggtcta gactgaggtg tctcacaagt gccgtgcagt 1740 cccgccccca cttgcttctc tttgtgtgta gtgtacgtac attatcgaga ccgttgttcc 1800 cgcccacctc gatccgggga tccctgaggt gtctcacaag tgccgtgcag tcccgccccc 1860 acttgcttct ctttgtgtgt agtgtacgta cattatcgag accgttgttc ccgcccacct 1920 cgatccgggt cgacctgagg tgtctcacaa gtgccgtgca gtcccgcccc cacttgcttc 1980 tctttgtgtg tagtgtacgt acattatcga gaccgttgtt cccgcccacc tcgatccggg 2040 agctcctgag gtgtctcaca agtgccgtgc agtcccgccc ccacttgctt ctctttgtgt 2100 gtagtgtacg tacattatcg agaccgttgt tcccgcccac ctcgatccgg tctagactga 2160 ggtgtctcac aagtgccgtg cagtcccgcc cccacttgct tctctttgtg tgtagtgtac 2220 gtacattatc gagaccgttg ttcccgccca cctcgatccg gggatccctg aggtgtctca 2280 caagtgccgt gcagtcccgc ccccacttgc ttctctttgt gtgtagtgta cgtacattat 2340 cgagaccgtt gttcccgccc acctcgatcc gggtcgacct gaggtgtctc acaagtgccg 2400 tgcagtcccg cccccacttg cttctctttg tgtgtagtgt acgtacatta tcgagaccgt 2460 tgttcccgcc cacctcgatc cgggcatgcc tgcagaagct tagagaccgg gttggcggcg 2520 tatttgtgtc ccaaaaaaca gccccaattg ccccaattga ccccaaattg acccagtagc 2580 gggcccaacc ccggcgagag cccccttcac cccacatatc aaacctcccc cggttcccac 2640 acttgccgtt aagggcgtag ggtactgcag tctggaatct acgcttgttc agactttgta 2700 ctagtttctt tgtctggcca tccgggtaac ccatgccgga cgcaaaatag actactgaaa 2760 atttttttgc tttgtggttg ggactttagc caagggtata aaagaccacc gtccccgaat 2820 tacctttcct cttcttttct ctctctcctt gtcaactcac acccgaaatc gttaagcatt 2880 tccttctgag tataagaatc attcaaaggc gcgccatggc taaggagtat ttccctcaga 2940 ttcagaagat caagttcgac ggcaaggaca gcaaaaaccc cctggctttc cattattacg 3000 acgccgagaa ggaagtgatg ggcaagaaga tgaaggactg gctgcggttc gcaatggcat 3060 ggtggcacac cctgtgcgca gagggagcag atcagttcgg aggaggcacc aagtcttttc 3120 catggaacga gggcacagac gccatcgaga tcgccaagca gaaggtggat gccggcttcg 3180 agatcatgca gaagctgggc atcccctact attgttttca cgacgtggat ctggtgtccg 3240 agggcaactc tatcggcgag tacgagtcca atctgaaggc cgtggtggcc tatctgaagg 3300 acaagcagaa ggagaccggc atcaagctgc tgtggagctc cgccaacgtg ttcggccaca 3360 agaggtacat gaacggcgcc tctaccaatc ccgactttga tgtggtggcc cgcgccatcg 3420 tgcagatcaa gaacgccatc gatacaggca tcgagctggg cgccgagaat tacgtgtttt 3480 ggggcggccg ggagggctat atgtctctgc tgaatacaga ccagaagaga gagaaggagc 3540 acatggccac catgctgaca atggccaggg attacgcccg cagcaagggc ttcaagggca 3600 cctttctgat cgagcccaag cctatggagc ctacaaagca ccagtatgac gtggataccg 3660 agacagccat cggcttcctg aaggcccaca atctggacaa ggacttcaag gtgaacatcg 3720 aagtgaatca cgccaccctg gccggccaca cattcgagca cgagctggca tgcgcagtgg 3780 acgcaggaat gctgggaagc atcgacgcca acaggggcga ttaccagaat ggctgggaca 3840 ccgatcagtt tcctatcgat cagtatgagc tggtgcaggc atggatggag atcatcaggg 3900 gaggaggatt cgtgaccgga ggaacaaact ttgacgccaa gacccggaga aatagcacag 3960 acctggagga tatcatcatc gcccacgtgt ccggaatgga tgcaatggca cgggccctgg 4020 agaacgcagc aaagctgctg caggagtccc catacaccaa gatgaagaag gagagatatg 4080 cctctttcga cagcggcatc ggcaaggact ttgaggatgg caagctgaca ctggagcagg 4140 tgtacgagta tggcaagaag aatggcgagc ctaagcagac ctcagggaag caggagctgt 4200 acgaagccat catcgcaatg tatcagtgaa tgcataccta gttaattaag gcacgtgcct 4260 aaaaaggatc gataccgtcg acctcgagtc atgtaattag ttatgtcacg cttacattca 4320 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag 4380 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt 4440 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt 4500 gagaaggttt tgggacgctc ggctaacttg tttaaacaac tgcaggcatg caagcttggc 4560 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 4620 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 4680 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 4740 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 4800 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 4860 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 4920 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 4980 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 5040 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 5100 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 5160 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 5220 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 5280 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 5340 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 5400 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 5460 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 5520 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 5580 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 5640 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 5700 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 5760 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 5820 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 5880 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 5940 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 6000 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 6060 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 6120 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 6180 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 6240 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 6300 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 6360 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 6420 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 6480 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 6540 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 6600 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 6660 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 6720 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 6780 gcccagatcc tctagagtcg aagcggccgc tatgtctgat aaaaggatgt aacataggca 6840 agctgctcgt gagtgttgag tacgaacctt agatccaaat cacccgcacc cacggatata 6900 cttgcttgaa tatacagtag tatgcggccg cttcgacacc atatcatata aaactaacaa 6960 tgcattgctt attacgaaga ctacccgttg ctatctccac accgttatct ccacggtcca 7020 aaggctgctc aatgtgctgc atacgtaacg tggggtgcaa ccttgagcac atagtacttt 7080 tccgaaaacc ggcgataatt aagtgtgcac tccaactttt cacactgagc gtaaaatgtg 7140 gagaagaaat cggcactaaa aagtcaggta gactggaaaa tgcgccatga aatgaatatc 7200 tcttgctaca gtaatgccca gcatcgaggg gtattgtgtc accaacacta tagtggcagc 7260 tgaagcgctc gtgattgtag tatgagtctt tattggtgat gggaagagtt cactcaatat 7320 tctcgttact gccaaaacac cacggtaatc ggccagacac catggatgta gatcaccaag 7380 cctgtgaatg ttattcgagc taaaatgcac atggttggtg aaaggagtag ttgctgtcga 7440 attccgtcgt cgcctgagtc atcatttatt taccagttgg ccacaaaccc ttgacgatct 7500 cgtatgtccc ctccgacata ctcccggccg gctggggtac gttcgatagc gctatcggca 7560 tcgacaaggt ttgggtccct agccgatacc gcactacctg agtcacaatc ttcggaggtt 7620 tagtcttcca catagcacgg gcaaaagtgc gtatatatac aagagcgttt gccagccaca 7680 gattttcact ccacacacca catcacacat acaaccacac acatccacaa tggaacccga 7740 aactaagaag accaagactg actccaagaa gattgttctt ctcggcggcg acttctgtgg 7800 ccccgaggtg attgccgagg ccgtcaaggt gctcaagtct gttgctgagg cctccggcac 7860 cgagtttgtg tttgaggacc gactcattgg aggagctgcc attgagaagg agggcgagcc 7920 catcaccgac gctactctcg acatctgccg aaaggctgac tctattatgc tcggtgctgt 7980 cggaggcgct gccaacaccg tatggaccac tcccgacgga cgaaccgacg tgcgacccga 8040 gcagggtctc ctcaagctgc gaaaggacct gaacctgtac gccaacctgc gaccctgcca 8100 gctgctgtcg cccaagctcg ccgatctctc ccccatccga aacgttgagg gcaccgactt 8160 catcattgtc cgagagctcg tcggaggtat ctactttgga gagcgaaagg aggatgacgg 8220 atctggcgtc gcttccgaca ccgagaccta ctccgttcct gaggttgagc gaattgcccg 8280 aatggccgcc ttcctggccc ttcagcacaa cccccctctt cccgtgtggt ctcttgacaa 8340 ggccaacgtg ctggcctcct ctcgactttg gcgaaagact gtcactcgag tcctcaagga 8400 cgaattcccc cagctcgagc tcaaccacca gctgatcgac tcggccgcca tgatcctcat 8460 caagcagccc tccaagatga atggtatcat catcaccacc aacatgtttg gcgatatcat 8520 ctccgacgag gcctccgtca tccccggttc tctgggtctg ctgccctccg cctctctggc 8580 ttctctgccc gacaccaacg aggcgttcgg tctgtacgag ccctgtcacg gatctgcccc 8640 cgatctcggc aagcagaagg tcaaccccat tgccaccatt ctgtctgccg ccatgatgct 8700 caagttctct cttaacatga agcccgccgg tgacgctgtt gaggctgccg tcaaggagtc 8760 cgtcgaggct ggtatcacta ccgccgatat cggaggctct tcctccacct ccgaggtcgg 8820 agacttgttg ccaacaaggt caaggagctg ctcaagaagg agtaagtcgt ttctacgacg 8880 cattgatgga aggagcaaac tgacgcgcct gcgggttggt ctaccggcag ggtccgctag 8940 tgtataagac tctataaaaa gggccctgcc ctgctaatga aatgatgatt tataatttac 9000 cggtgtagca accttgacta gaagaagcag attgggtgtg tttgtagtgg aggacagtgg 9060 tacgttttgg aaacagtctt cttgaaagtg tcttgtctac agtatattca ctcataacct 9120 caatagccaa gggtgtagtc ggtttattaa aggaagggag ttgtggctga tgtggataga 9180 tatctttaag ctggcgactg cacccaacga gtgtggtggt agcttgttac tgtatattcg 9240 gtaagatata ttttgtgggg ttttagtggt gtttggtagg ttagtgcttg gtatatgagt 9300 tgtaggcatg acaatttgga aaggggtgga ctttgggaat attgtgggat ttcaatacct 9360 tagtttgtac agggtaattg ttacaaatga tacaaagaac tgtatttctt ttcatttgtt 9420 ttaattggtt gtatatcaag tccgttagac gagctcagtg ccatggcttt tggcactgta 9480 tttcattttt agaggtacac tacattcagt gaggtatggt aaggttgagg gcataatgaa 9540 ggcaccttgt actgacagtc acagacctct caccgagaat tttatgagat atactcgggt 9600 tcattttagg ctccgattcg attcaaatta ttactgtcga aatcggttga gcatccgttg 9660 atttccgaac agatctggcc ctttcgtc 9688 <210> 4 <211> 9394 <212> DNA <213> Artificial Sequence <220> <223> pMCS-UAS1B12-TEF-XK-CYCt <400> 4 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccgctttt 420 cgtagataat ggaatacaaa tggatatcca gagtatacac atggatagta tacactgaca 480 cgacaattct gtatctcttt atgttaacta ctgtgaggcg ttaaatagag cttgatatat 540 aaaatgttac atttcacagt ctgaactttt gcagattacc taatttggta agatattaat 600 tatgaactga aagttgatgg catccctaaa tttgatgaaa gggcgcggga tccggcccgg 660 gaattcgaag gtaccaagga agcatgcggt acccgaattc ctgaggtgtc tcacaagtgc 720 cgtgcagtcc cgcccccact tgcttctctt tgtgtgtagt gtacgtacat tatcgagacc 780 gttgttcccg cccacctcga tccggtctag actgaggtgt ctcacaagtg ccgtgcagtc 840 ccgcccccac ttgcttctct ttgtgtgtag tgtacgtaca ttatcgagac cgttgttccc 900 gcccacctcg atccggggat ccctgaggtg tctcacaagt gccgtgcagt cccgccccca 960 cttgcttctc tttgtgtgta gtgtacgtac attatcgaga ccgttgttcc cgcccacctc 1020 gatccgggtc gacctgaggt gtctcacaag tgccgtgcag tcccgccccc acttgcttct 1080 ctttgtgtgt agtgtacgta cattatcgag accgttgttc ccgcccacct cgatccggga 1140 gctcctgagg tgtctcacaa gtgccgtgca gtcccgcccc cacttgcttc tctttgtgtg 1200 tagtgtacgt acattatcga gaccgttgtt cccgcccacc tcgatccggt ctagactgag 1260 gtgtctcaca agtgccgtgc agtcccgccc ccacttgctt ctctttgtgt gtagtgtacg 1320 tacattatcg agaccgttgt tcccgcccac ctcgatccgg ggatccctga ggtgtctcac 1380 aagtgccgtg cagtcccgcc cccacttgct tctctttgtg tgtagtgtac gtacattatc 1440 gagaccgttg ttcccgccca cctcgatccg ggtcgacctg aggtgtctca caagtgccgt 1500 gcagtcccgc ccccacttgc ttctctttgt gtgtagtgta cgtacattat cgagaccgtt 1560 gttcccgccc acctcgatcc gggcatgcgg tacccgaatt cctgaggtgt ctcacaagtg 1620 ccgtgcagtc ccgcccccac ttgcttctct ttgtgtgtag tgtacgtaca ttatcgagac 1680 cgttgttccc gcccacctcg atccggtcta gactgaggtg tctcacaagt gccgtgcagt 1740 cccgccccca cttgcttctc tttgtgtgta gtgtacgtac attatcgaga ccgttgttcc 1800 cgcccacctc gatccgggga tccctgaggt gtctcacaag tgccgtgcag tcccgccccc 1860 acttgcttct ctttgtgtgt agtgtacgta cattatcgag accgttgttc ccgcccacct 1920 cgatccgggt cgacctgagg tgtctcacaa gtgccgtgca gtcccgcccc cacttgcttc 1980 tctttgtgtg tagtgtacgt acattatcga gaccgttgtt cccgcccacc tcgatccggg 2040 agctcctgag gtgtctcaca agtgccgtgc agtcccgccc ccacttgctt ctctttgtgt 2100 gtagtgtacg tacattatcg agaccgttgt tcccgcccac ctcgatccgg tctagactga 2160 ggtgtctcac aagtgccgtg cagtcccgcc cccacttgct tctctttgtg tgtagtgtac 2220 gtacattatc gagaccgttg ttcccgccca cctcgatccg gggatccctg aggtgtctca 2280 caagtgccgt gcagtcccgc ccccacttgc ttctctttgt gtgtagtgta cgtacattat 2340 cgagaccgtt gttcccgccc acctcgatcc gggtcgacct gaggtgtctc acaagtgccg 2400 tgcagtcccg cccccacttg cttctctttg tgtgtagtgt acgtacatta tcgagaccgt 2460 tgttcccgcc cacctcgatc cgggcatgcc tgcagaagct tagagaccgg gttggcggcg 2520 tatttgtgtc ccaaaaaaca gccccaattg ccccaattga ccccaaattg acccagtagc 2580 gggcccaacc ccggcgagag cccccttcac cccacatatc aaacctcccc cggttcccac 2640 acttgccgtt aagggcgtag ggtactgcag tctggaatct acgcttgttc agactttgta 2700 ctagtttctt tgtctggcca tccgggtaac ccatgccgga cgcaaaatag actactgaaa 2760 atttttttgc tttgtggttg ggactttagc caagggtata aaagaccacc gtccccgaat 2820 tacctttcct cttcttttct ctctctcctt gtcaactcac acccgaaatc gttaagcatt 2880 tccttctgag tataagaatc attcaaaggc gcgccatgtc caatttactg accgtacacc 2940 aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg 3000 acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt 3060 gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg 3120 aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc 3180 agcaacattt gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg 3240 acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg 3300 aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca 3360 tggaaaatag cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata 3420 acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg 3480 acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg 3540 tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg 3600 gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg 3660 cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc 3720 atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac 3780 acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga 3840 tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaccctgg 3900 atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgattagtta attaaggcac 3960 gtgcctaaaa aggatcgata ccgtcgacct cgagtcatgt aattagttat gtcacgctta 4020 cattcacgcc ctccccccac atccgctcta accgaaaagg aaggagttag acaacctgaa 4080 gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt 4140 caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc 4200 ttgcttgaga aggttttggg acgctcggct aacttgttta aacaactgca ggcatgcaag 4260 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 4320 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 4380 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 4440 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 4500 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 4560 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 4620 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 4680 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 4740 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 4800 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 4860 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 4920 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 4980 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 5040 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 5100 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 5160 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 5220 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 5280 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 5340 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 5400 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 5460 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 5520 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 5580 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 5640 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 5700 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 5760 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 5820 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 5880 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 5940 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 6000 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 6060 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 6120 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 6180 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 6240 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 6300 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 6360 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 6420 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 6480 cacgaggccc agatcctcta gagtcgaagc ggccgctatg tctgataaaa ggatgtaaca 6540 taggcaagct gctcgtgagt gttgagtacg aaccttagat ccaaatcacc cgcacccacg 6600 gatatacttg cttgaatata cagtagtatg cggccgcttc gacaccatat catataaaac 6660 taacaatgca ttgcttatta cgaagactac ccgttgctat ctccacaccg ttatctccac 6720 ggtccaaagg ctgctcaatg tgctgcatac gtaacgtggg gtgcaacctt gagcacatag 6780 tacttttccg aaaaccggcg ataattaagt gtgcactcca acttttcaca ctgagcgtaa 6840 aatgtggaga agaaatcggc actaaaaagt caggtagact ggaaaatgcg ccatgaaatg 6900 aatatctctt gctacagtaa tgcccagcat cgaggggtat tgtgtcacca acactatagt 6960 ggcagctgaa gcgctcgtga ttgtagtatg agtctttatt ggtgatggga agagttcact 7020 caatattctc gttactgcca aaacaccacg gtaatcggcc agacaccatg gatgtagatc 7080 accaagcctg tgaatgttat tcgagctaaa atgcacatgg ttggtgaaag gagtagttgc 7140 tgtcgaattc cgtcgtcgcc tgagtcatca tttatttacc agttggccac aaacccttga 7200 cgatctcgta tgtcccctcc gacatactcc cggccggctg gggtacgttc gatagcgcta 7260 tcggcatcga caaggtttgg gtccctagcc gataccgcac tacctgagtc acaatcttcg 7320 gaggtttagt cttccacata gcacgggcaa aagtgcgtat atatacaaga gcgtttgcca 7380 gccacagatt ttcactccac acaccacatc acacatacaa ccacacacat ccacaatgga 7440 acccgaaact aagaagacca agactgactc caagaagatt gttcttctcg gcggcgactt 7500 ctgtggcccc gaggtgattg ccgaggccgt caaggtgctc aagtctgttg ctgaggcctc 7560 cggcaccgag tttgtgtttg aggaccgact cattggagga gctgccattg agaaggaggg 7620 cgagcccatc accgacgcta ctctcgacat ctgccgaaag gctgactcta ttatgctcgg 7680 tgctgtcgga ggcgctgcca acaccgtatg gaccactccc gacggacgaa ccgacgtgcg 7740 acccgagcag ggtctcctca agctgcgaaa ggacctgaac ctgtacgcca acctgcgacc 7800 ctgccagctg ctgtcgccca agctcgccga tctctccccc atccgaaacg ttgagggcac 7860 cgacttcatc attgtccgag agctcgtcgg aggtatctac tttggagagc gaaaggagga 7920 tgacggatct ggcgtcgctt ccgacaccga gacctactcc gttcctgagg ttgagcgaat 7980 tgcccgaatg gccgccttcc tggcccttca gcacaacccc cctcttcccg tgtggtctct 8040 tgacaaggcc aacgtgctgg cctcctctcg actttggcga aagactgtca ctcgagtcct 8100 caaggacgaa ttcccccagc tcgagctcaa ccaccagctg atcgactcgg ccgccatgat 8160 cctcatcaag cagccctcca agatgaatgg tatcatcatc accaccaaca tgtttggcga 8220 tatcatctcc gacgaggcct ccgtcatccc cggttctctg ggtctgctgc cctccgcctc 8280 tctggcttct ctgcccgaca ccaacgaggc gttcggtctg tacgagccct gtcacggatc 8340 tgcccccgat ctcggcaagc agaaggtcaa ccccattgcc accattctgt ctgccgccat 8400 gatgctcaag ttctctctta acatgaagcc cgccggtgac gctgttgagg ctgccgtcaa 8460 ggagtccgtc gaggctggta tcactaccgc cgatatcgga ggctcttcct ccacctccga 8520 ggtcggagac ttgttgccaa caaggtcaag gagctgctca agaaggagta agtcgtttct 8580 acgacgcatt gatggaagga gcaaactgac gcgcctgcgg gttggtctac cggcagggtc 8640 cgctagtgta taagactcta taaaaagggc cctgccctgc taatgaaatg atgatttata 8700 atttaccggt gtagcaacct tgactagaag aagcagattg ggtgtgtttg tagtggagga 8760 cagtggtacg ttttggaaac agtcttcttg aaagtgtctt gtctacagta tattcactca 8820 taacctcaat agccaagggt gtagtcggtt tattaaagga agggagttgt ggctgatgtg 8880 gatagatatc tttaagctgg cgactgcacc caacgagtgt ggtggtagct tgttactgta 8940 tattcggtaa gatatatttt gtggggtttt agtggtgttt ggtaggttag tgcttggtat 9000 atgagttgta ggcatgacaa tttggaaagg ggtggacttt gggaatattg tgggatttca 9060 ataccttagt ttgtacaggg taattgttac aaatgataca aagaactgta tttcttttca 9120 tttgttttaa ttggttgtat atcaagtccg ttagacgagc tcagtgccat ggcttttggc 9180 actgtatttc atttttagag gtacactaca ttcagtgagg tatggtaagg ttgagggcat 9240 aatgaaggca ccttgtactg acagtcacag acctctcacc gagaatttta tgagatatac 9300 tcgggttcat tttaggctcc gattcgattc aaattattac tgtcgaaatc ggttgagcat 9360 ccgttgattt ccgaacagat ctggcccttt cgtc 9394 <210> 5 <211> 1386 <212> DNA <213> Artificial Sequence <220> <223> YALI0_A00891g <400> 5 atggggggga aaaacgggga aatcaaggta tcggggcggg gaaaagggcg aatttggtta 60 tttggggtca aggaaaaacg ctggcgggct cgtattcccg ataaaaccgc tccgggactc 120 gcttttcagg tagcccgcac cggcgaaaaa gccagggatt gggtgaaaac attaaggttc 180 ggccctcaaa gttcacagtg gtcagcgcag tgtaaacacg tcgacccaca cacaccactc 240 cgccataacc acatctctcg agctcgctgt agacacaaga gcttccgtca cccgcatcca 300 tctcacagac acagacacac acccacacat tcacgcatca ccagacccac aagaaccgcc 360 acaatggaag attcagacgt gctgatgaga gatatcaacg acgacggact caagcccatg 420 gacgccatgg gagccggggg cgaagggacg ttcacagaac tgacacccac aacccgcggc 480 cagctggacg cctggatcgc acgactgacc gaatgccagc ctctcagcga agacgacgtc 540 aacgagctgt gcaacatggc caagggggtg ttgcagaagg aggaaaacgt gcagcccgtg 600 cacgcgccgg tgaccatctg tggagacgtg cacggccagt tccacgacct gatggagctg 660 ttcaaaatcg gcgggccgtg tccagacaca aactacctgt ttatgggaga ctacgtggac 720 cgaggctact actcggtcga aacggtgtcc tatctggtgg ccatgaaggt gcgatacccc 780 aaccgactga ccattctgcg aggaaaccac gagtcgcgac aaatcaccca ggtctacggc 840 ttctacgacg agtgtctgcg aaagtacgga aacgccaacg tgtggcgagc cttcacgtcg 900 ctgttcgact acttccccgt caccgccctg gtggagaacc aaatcttctg tctgcacgga 960 ggcctgtctc ccatgatcga gtccatcgac aacatccgag aactcaaccg attccaggag 1020 gtgccccacg acggccccat gtgcgacctg ctgtggtccg accccgatga ccgaggagga 1080 tggggaatga gcccccgagg agccggcttc accttcggtg ccgacatttc cgaacagttt 1140 aaccacacaa acaacctgag tctcatttca agagcacacc agctggtcat ggaaggcttc 1200 agctgggcac acgagcagca ggtggtcacc atcttctccg cccccaacta ctgctaccga 1260 tgcggaaacc aggccgccat catggaggtg gacgaaaacc tctcaaactc ctttttgcag 1320 tacgatcctt gtccccgtcc cggagagccc tccgtgtctc gacgaacccc cgactacttc 1380 ttgtag 1386 <210> 6 <211> 325 <212> DNA <213> Artificial Sequence <220> <223> YALI0_A00935g <400> 6 atgaaaataa tcaacatgtt gtctttgggg ggcaacactc tgaccggcaa cactctgacc 60 ggcaacactc tgaccggcaa caccctgact ggcaatactt cgaccgcctc tcacttttca 120 tcattaatcc tcgcccattt cctcaacaac attccttctt cgaactctgc tcatcttcga 180 actctgctca tcttcgggct ctgctcatct tcctgctact ctcttccagt tcccctggca 240 tacctccaac atccatcacc aagaccaata catacatgtt tcgtctcgat gcgatcaata 300 ttctcttttg gaatgcagag ggtga 325 <210> 7 <211> 1344 <212> DNA <213> Artificial Sequence <220> <223> YALI0_A02002g <400> 7 atgaaaccag tcgcacttct cacatcctgt gtggctacat ttgccgtgct taatggagcc 60 attgcggccc cttcagcccc caaagtgctc cagatccccg tcacaaagca ccacgagtct 120 gctgctgagg tcaaccggac cctcgctcta gctcgtcgag acggagcgta caccgaaacg 180 gtccacaaca aggagttctg gtactccatc tccatctcgc taggaacacc ggcccaacag 240 ttcaacgtgt tgctggacac gggctcgtca gacctgtggg tgttcagcac ggaagactcg 300 accgactgcg ctaacggagc ctgcgaattc acaggccagt tcaacgcaaa gtcgtcttct 360 acctaccact acctcaacag cgactacagc atcacgtacg tgacggggtc ggctcatgga 420 gactgggtca cggactcgct ttcggtcggc cccgtgactc tcagcaactt tcagtttgcc 480 gtggccgaca aggcggtggg caatactgcc atcttcggta tctctctgga gggatccgag 540 tctctgaacc atggacaaca gcccgagtat cccaactttc ccgtccagct caagaaccag 600 ggcttcatcg acagaatagt ctactctctt tacctggaag atgtcaattc gccccagggc 660 actcttctac tcggaggaat cgactacgcc aagttccagg gtcctctcaa tgtgcttcag 720 cttcagaacg ccaacgcttt ccaggtcgag taccagggag tcggaatcaa cggagctggg 780 ccctacggac agcctcacgt ggcggttttg gactcgggaa cttcatacac gtttctgccc 840 gatgacatct actacaccat cttcgaccag gtcggactat caagtcaagt gaaccagaac 900 accggtctca actacgtcgc atgtaacaca cgcgttactc tggcgtttga tttcggaaat 960 ggagccatca tcaatgtcga ctctagcgag ctggttctca agctgagcga cattcttggt 1020 gatccccaca acaaccagtg cgtctttgga gtctcatcca atgacaacac ccacggtatc 1080 acccttttgg gagacacctt tctgcgatct gcatatgtgg tttacgacat tgaaaacaag 1140 gaggttggta ttgctcaggc agtgtatacc agcaactcca acgttcagcc cgtcacaggt 1200 gctcttggag gacagggtca gcagcagagt caaggtcaag gtcaaggtca aggtcgaggt 1260 caaggtcagg gacagcagag tcaaggccag ggtcagggac aatatccctg ggatggacaa 1320 ccttttggat caggtttctt ctaa 1344 <210> 8 <211> 925 <212> DNA <213> Artificial Sequence <220> <223> YALI0_A02497g <400> 8 atgaaccgaa acgactacga cgacaaccga ggcaacagcc gaaacgactc ctacggcaac 60 cagggccgaa acgagggccg aaacgagtcc tacggaaaca acaactccaa ctacggcaac 120 aactccaact ccaactccaa ctctggtttt ggtggtaaca actccaactc tggattcggc 180 ggtaactcca actccaactc caactacgga aacaactcca actccaacta cggaaacaac 240 tccaactcca actccaacag ctcctacgga tccaactcca actccaacca tggtggcaac 300 gattcctacg gtaacaactc caactccaac tccaactccc actacggaaa caactccaac 360 tccaactcca actccaactc caactacgga aacaactcca actccaactc caactacgga 420 aacaactcca acaactccaa ctccaactcc aactccaact ccaactccaa tgtcaacccc 480 tacgttgcca agggtatcaa catggtggag gaaaagttct ttggaatgga ccagaagaac 540 gagactgcca acgagcgaaa gtacgaccag aagattggcg agttcatcac ctccaagatt 600 ggcggcggca acaagggctc ctccaactct gcaaacaact ccaacaactc ttacggaaac 660 aacaacaact ccaacaactc caacaactcg tcttccggcg gaattggcgt gagtattaca 720 acgatagatt gatctgttgc agcactacac aggactgctc caatcatggt taattgacca 780 caactaacat cagggcttca tttctagcca gctgtctgga aactccaact ccaacaacaa 840 ctccaacaac tacaacaaca acaacaacaa caacaacaac agtggaggtg acagccttca 900 ggacaagctt ggcgtgagta tttaa 925 <210> 9 <211> 4266 <212> DNA <213> Artificial Sequence <220> <223> YALI0_A07997g <400> 9 atgcagtact gtactaacgc aggaactaga gcccggtcct cgtccacctc ttcagtgcaa 60 aaccagccgt caaagctctc tctcggtctc cagcatgtca aggaagaagc tgattcgctt 120 tcatcctcac gggccacgtc gccaggtacc actcccacaa acgttgcatc ttccagcccc 180 tctgcatcaa agacggcact ggagccctcc acctcctcac acgacttgtc taacacccgt 240 tccctggacc cctacgtcaa gacctctgtc ggaagcccca acctgtctcg ttcacgcacc 300 atttctgcgc ccgagttcaa gtccgagcgt gaaaagctca acaaggagca gtcgccaggc 360 ttcaaaaagt cgtggctcaa ctccttcagc aagtggagat ctggcaaaga cgaaaagact 420 aaagatggca aggatgcatc ctcgtcgtcg ttaggaaaga gccctacagc cacgcctttc 480 tcgaaaccca gcaaatcaca acccattgca tccaaggagc agcaaacgtc ggcatcgcta 540 tcaggcaagg ctgcccccac gacacaaacc accacaacga cgattccaga aatcgctcct 600 cctgtcgctg ctgccacagg agccactacc gtccctcctg catctactcc cacctctgcc 660 aacctcccga cttcaccccc tcggacagcc tctcctcgta tcaatgttga ggccgttgcc 720 atcccagaga agcccttccc gcctgtccac gcagcaggcg cctcaccggc ggtggcctcg 780 tccatcgcct cttctgcatc cacagatgtg ggatcgccca aggggttcct tctcaacact 840 ctgcggcggt tctccaagtc gcagccctcc acaggaggcg tagcgtcgtg tcccacgtcg 900 cgtgttttga acaagaacag taaccgacgg aattgtgagc tcacgggtct tgagggcgtc 960 tcttctcgtc gagtgtcgtt tgatatcaac acgtatcatt cggaccctcc tcagcagatc 1020 cccgcccgga agcccaagaa gggtaatgta gaggttggtg ccgatggttt gctccgtaag 1080 aatggcatgg tgatccaatc accccctcag caccatgacc agcatcactt caacccattc 1140 aattcgggtg ttggacagcc cagacggcag gcggcaaaga cggctcctcc tgccacttca 1200 tccaagaccc cacagacggc agctcctgct caggcggctc aaacgcagac aacaacccct 1260 caaaagcctg ccactgcctc tccggcagcc caagcagctc cagttgcagt tcccaaacct 1320 gcagcacccg ctcccgcagc agcagcagca gcagtcccca aagagcccgt tgctcccaaa 1380 ataagccctc ctactgctgc gacggctgga gctgcttctg ccgcagcagc agcaatcccc 1440 tccaatggtg gctcctcagt cgcgacttcc acagtctctg ctgtaccttc cactggagct 1500 gtctccaacg gagtctcgtc ttctgcttcc tccacttcgt ccacgtcatc gacctcttcc 1560 aacaactcct ccctcacagt ccctaccggc gctcctgttc gagcatcctc tcccaatggc 1620 aagtctcctc ttggaaaacc gtcccaaccc catctcacag tagacatgca gaaggtcggg 1680 ggtcgctcac ccgtagggtc gcctcgagaa tcgccttctc cctcgcctac gacctctggt 1740 ctctcacgat ccaattctgg acgttccaag tttgttatca tgcgaaatgg taaggaggtg 1800 gagttcacca aggcgtcggt ctacaataag gagaacactc tggctcgttc gggctcactg 1860 accaagaaac gatcgccccg aaactcgccc aaaaactctc ctattgctgg ctctcctcgg 1920 tctgcatctc ccctggcagc caaggcgtca cccagcaagc ccccctcacc tactaaggaa 1980 actgccaagg acagtaccaa agacactaaa acgtctcctc ctccgggcga cgacgaggtg 2040 ccctctatct ccaacctcat tatcgacacg cctctcaaga catcccatga aatgtacaac 2100 gagaaggacg agtcggacga ggacaacccg ctggatgtgg acgtagagaa actgtatact 2160 cggtgctgtc atctgcgaga gattctgccg atcccagcta ctctaaaaca gatcaaggcc 2220 caaaaggcgc ctctttccct gcgtctgatg aatcccagac ccacgttgat tgagatccag 2280 tcatttgccg actttgtggc tgtggctccg gtcagtacac ttgttctgga taatgtgacc 2340 ctcactccgg atatgttttc actgttgttt gcggcaatga gtgcttcgca atggctcgaa 2400 aagctgtctc ttcgaaacac tagcattgac gagatgtcat ggaagcgcct gtgctacttg 2460 ttggccatga actccaagct tgttcggctg gacttgtcgc atcagatgaa gcgggacaag 2520 gacaaggagc tcaagcagcg aacccctccc actgtggatt ggaagctgtt gtgcgatgct 2580 ctacagtacg gcaaaggtat ggaggaactt gtgctcaacg gctgtttagt gccttctgag 2640 gtgtttgagc atctcatgtt gggcgcatgc aaaaatacaa aacgtctcgg actagcattc 2700 aacgatttgg gccccgagga gatgcatttc gttggccaat ggatgactgg cgagtgtgag 2760 ggtctcgata ttggaggtaa ccatctgact tacgtggagg aggaggagga ggtgagacaa 2820 gaaggagccg aaggtacaga gaagggggag caggaggaga aggaggagaa ggaggagaag 2880 gaggaggagg aggagaagga ggagaaggag gagaagaagg ataaggtgcc cgtttccgaa 2940 tcaaccgaac ctaccgctct tgatgttctt ctggattcac tctccgacaa ggagctgaac 3000 ctgcggttcc tttccctgaa ctccacgcac ctcgaggcct ctcccacgac cgaaaagctc 3060 atcactgccc tttccaagct gcagaatctc cggtttttag atctctcttc taactctgga 3120 ctctttccct cccttacgac tcatctgtgt aaagctctac cgcttttccc agatttgcgc 3180 cgaattcatc tcgattactg cgacttgagc cctcgtgatg tggtgcagat ttcagacgct 3240 ttggcgcaat gtaagtcgct tctgcatgtt tcccttgttg gaaacacgcg catggaccga 3300 acttgtgtgg cagctctgac tgtagccgtt cgggtttcaa agtccatcta ccttcttgac 3360 cttgaacaga gtctcgtacc caagatggag cagcgacgaa tcgtgcatta ctgcatgcgc 3420 aacatggagc ggttggtata cggcaaggca acctctgaaa acgagattga ccatgagatt 3480 gaggtcggag aagacaaccg ggatatcttt gacgtgggta aggacattgc ttctactgtc 3540 gatgatattc tcgagtttaa ccaggagacg tgccaggtta gtgacatgct acttcagcgt 3600 gcctaccacg ttaagactcg ggttcagaag gttatggacg agctgtttgc caagcgaaac 3660 aacggcgagc tgacctacga ggacaaggag ctgcttgttc gttactgttt cctcgatgct 3720 actttgaacg agactcttca gaactttgag aagcagcgaa agcagcccca gacccagggt 3780 cccaccgtcc atcctctgac tcacatgatg ggcctggcag gcaacgaacc tcagatcaag 3840 gctcctgatc tccaggttga gggagtggat gaaggtcgtc ccgctctttc ccgaaactcg 3900 tcgtcggcgt ctatccaggc tcgacgacag gagctcgagg agggagagtt tcacaagtgg 3960 ggaacttttg tgatgcaggt tcgagagaac gagccccttc ttcactctgg ccgaccttct 4020 ggagatgatc tgcgtaaggc gattctcaat gccaccggtg tgggcactgt ttccgagttg 4080 attggcgaca ttattcattc tggagtggat gtcgaggagt tactcaacat ttctggtctg 4140 gatcagtacc gaaagacttt ggctgaggag actgaggatg gatctgctgt tcctatggag 4200 gttaaggccg agcgagatgc cattgacagt ttcttggagg atctggcccg ggtcagaagc 4260 agatga 4266 <210> 10 <211> 277 <212> DNA <213> Artificial Sequence <220> <223> YALI0_A13849g <400> 10 atgaaactgg aagcacacga cacaccgtca gcaagacatt ttggatactg caggtcgttt 60 gttagattgt cccataatct gctattggtc aagaatgatc aaggagatga gagagtatac 120 gaggttgtgc gacaagtgcc aatggttcat gaaagggacc acagccaaaa aaaaaaatgt 180 tctcaagcag ctgcctggac ctacgaagat ctggtcggaa atttcgaagg attttgggtc 240 agtcgaggct ccgaaagtca aggaaccgat tccataa 277 <210> 11 <211> 232 <212> DNA <213> Artificial Sequence <220> <223> YALI0_A15642g <400> 11 atgtctaggt gtcgcaacga cgtggacgcg attgccacat gggatattgc caaagattcc 60 agtgtgcggc gtgggatgaa ggcgctgttt gaagaaaagc tggacacggg ggtccttgaa 120 gactaccagc tgaagcgaac cagctgagag cgaaccagct ggagcgaacc agttggagcg 180 aaccagttgg agcgaacaca tctggtgggt atgcatacga atgtggatct ga 232 <210> 12 <211> 2952 <212> DNA <213> Artificial Sequence <220> <223> YALI0_A15796g <400> 12 atgaaactct catctgccgc cttggtggcc ttcgcagctc ctgtgattgc ccagcagacc 60 accaacacgt atgcaactac aactatcact agcatgcaca cggtcacggc tacactgacc 120 tgtgagccct ccgactacgt caccagtacc cgaaccctta ttgagtatga agatgataga 180 tgcatcgtca acaactttgt ctactcacac tgcaagttcc tccccccttc taccacctgt 240 ccccccgagt tccagacttg ggtcacaaag accacggaaa cggagtcggg gttttctatt 300 tggaccactg aagtcgtggt gatggagtgc ttatctacac ctgtcagtgt ccccatcaca 360 accaccgatc ctgccaccat tcctgagtcc accgagcctg caacttccac cgagtccacc 420 actagtccag agacttctcc accaatctca gaggagccca gcacttcgga ggagcccact 480 acttcggaga agcccattac ttcggaggag cccactacat cccctgagac ctctaagcag 540 cccacgacct ctgagcagcc caccactaca tcggaggagc ccactacttc ggaggagccc 600 actacatcgg aggagcccac gactacatcg gaggggccca ctacatcgga agagcctact 660 acatcccctg agacctctga gcagcccacg acctctgagc agcccacgac ctctgagcag 720 cccacggcta catcggagga gcccactaca tcggaagagc ctactacatc ccatgagacg 780 tctgagcagc ccacgacgtc tgagcaacca actacatccc ctgaaacttc tgctcctacc 840 actaatgagg agccgacatc ttcaagtcct gttacagacc cttgcaccac tgtcaccacc 900 atcgtgacca cacccccagg ggaagaacct acaacctaca caacaactgt tgacacctgt 960 tccagtgatc ctactcctca gccacaaact tccggatcta ccaccacaga tccttgcttg 1020 gagaccacca ctattgtttc tactcccatc accggcgagc ccagcacttt caccatcact 1080 acagacgtgt gctcgtcgtc cgttcccatc acagagtgtg tgacgaccat tgtctcaaat 1140 cctccaggtg aagtgcctac taccctgacg gttacgaccg acgtttgcac tcccgagcca 1200 actacagaga taaccagtgc tccagttacc gagtgcacca ctacagtggt ttctacacct 1260 ccaggtggtg atcctaccac catgaccgtt accactgacg tttgcactcc cgaaccagtg 1320 acctccgagc cagtgacctc cgagccagtg acctccgagc cagtgacctc cgagccagtg 1380 acctccgagc cagtgacctc cgagccagtg acctccgagc cagtgacctc cgaaccagtg 1440 acctccgaac cagtgacctc cgaaccagtg acctccgaac cagtgactcc tacagagtgc 1500 attacaaccg tggtctcgac tcctccaggt gaagagccta ctaccctgac gattacgact 1560 gacatttgca ctaccgagcc ccctactcag acaaccagtg cccccgttac ggagtgcaca 1620 actacagtgg tttctactcc cccaggaggc gaccctacta ccctgacgat taccactgac 1680 gtttgtactc ctgagccagt gacttcggag ccagtgtccc ccacagagtg tatcacgacc 1740 gtggtttcaa cccctcccaa tggtgagccc accacactga cggtcacaac agattactgc 1800 cctcctgtga gcactcctgt ggtccctacc gactgtgtca caaccacaac tgtcaccacc 1860 actgatcctg ctggcgagcc ttccactgtg accaccacaa tcgataactg ccctacacct 1920 gaacccgtta ccaccaccga ctgcatcacc actacagttg tgaccaccgt aactgtttgt 1980 gaagacgagt gcactaccat ccccgtgact attactacag aagttccttc cctgcactct 2040 tccaccacca ttgtcaccac tatcgaaaac aagaccgtga ctctgactgt tccttgtgat 2100 cccgaagaga ccccctccat caccgagccc gtcgaggtgc cttctgtccc caccactgtc 2160 accgagatcc ttactgagac cacttcaata tgtgataacg aggagtgtta tgagaccact 2220 acagtggtaa ccaagacagt tgtccattct gtgcctgtcg aggagatcaa gcccactcct 2280 gaggttgaga agcctactcc tgaggttgag aagcctactc ctgaggttga gaagcctacg 2340 cccgagaagc ctactcccga ggttcctgag aagcctactc ccgaggttcc tgagaagcct 2400 actcctgagg ttgagaagcc tacgcccgag aagcctactc ctgaggttcc tgagaagcct 2460 acgcccgaga agcctactcc cgaggttcct gagaagccta ctcctgaggt tgagaagcct 2520 acgcccgaga agcctactcc tgaggttcct gagaagccta ctcctgaggt tgagaagcct 2580 acgcccgaga agcctactcc tgaggttcct gagaagccta ctcctgaggt tgagaagcct 2640 acgcccgaga agcctactcc tgaggttccc cagagtgaga agcctacgcc cgagaagcct 2700 acgcccgaga aacccacacc tgaggttccc gagcccgaac agccctctcc ttctcccgag 2760 aattcctctc ctcatcccca gcatccctct tctcctgaga cttccactcc tagtcagcca 2820 ggcgagcaac ccgtccccga gcagtctgtc cctgaaactc ctggatctgg cccagaacag 2880 gccaacctgg ctgttactct caaggccacg actctagctt cactgtttcc gctgatggct 2940 cttctcatct ag 2952 <210> 13 <211> 913 <212> DNA <213> Artificial Sequence <220> <223> YALI0_A16863g <400> 13 atggatccct ggctgcgaga tctggtagcg ggaacggtgg cgggctccgt gtccaccgtg 60 ttcatgcatc cgctggacct gctgaaaatc cgactgcaac tggacggaaa cctggggacg 120 gtgctgagat cgctgcgaca gtcggacgga ccctacgcgg ggaaatttcg gggcctgtac 180 aagggcctgt atcgcggtct gggcatcaat ttactcggaa acgccgcggg atacggcgtc 240 tacttttctc tctacggcat cgtcaagaaa atgcacctgt ttgatggacc ccacggctac 300 tttttcaacg ctctcatcac aggaacagcc acaagcatag cgaccaaccc gctgtgggtg 360 ctcaagacgc gaatctgctc gacaaacgcc ggccatgtgg acgcatactc ctccatgctc 420 gacggagtca aacggatcta cagccaggaa ggaatcaagg gcttctggag aggccagatc 480 ccgtctctgc ttggagtggt gcaggccgca gttcagtttg gattctatga ctgggccaag 540 gaacaagtca agctagcgcg gtcgcgcgac ccctccaaca gctacgacat ttcactcaca 600 aaagaagggg ctcccagtta tttgtcaacc aaggagtact tattgctgtc gtccacgagt 660 aaggctgtgt ccactgtttt gctgtatcct taccaggtgg tgcgttccaa gctccaacga 720 tacgatgccg gaaagatgta ctcttccatt ggtgactgta tcagcaagat atacagcaat 780 ggcggcttct ttgccttcta tcgaggtcta gtgccgaatt tgcttcgcgt tctgccagcc 840 acctgcatca cttttgtcgt ctatgaaaag gtcaacgagc agttggagga gctgagatga 900 cgaggtgaga tga 913 <210> 14 <211> 3711 <212> DNA <213> Artificial Sequence <220> <223> YALI0_A17578g <400> 14 atggcgaaaa aacgaaagaa caagaccggc aaggtccagg atcgggccgc cgacgaccag 60 ccggaggacg cggttgcaga tcagcacgtg gacaagacgg aagactcgga accagacaat 120 caggagcacc cagccacggg cccaaatgac gagatccagg acccggcggg cgacaaggtg 180 gaagacacgg tggacaacgt ggaaaacgcg gacgacacgg acaaaccgga cctggatgaa 240 tcagacaaat cagtccagac gggatcacaa gactcacgca actcggaaaa gacagatcaa 300 aagaccgaat caaccgccga atcaaccgaa tcaaccgaat cgactgaatc gaccgttccg 360 aacaaaccca atctcttttc tctttctgac gaagacgatt ccgaagacga gccgtcggag 420 tcgtcggagc cggcggagcc gtcagagccg gtcaaccctg tacctgtcga gactgtcgag 480 tctcccgtca agacccaacc tgagaacaag ggctcgtctc tcaatgactc ttctatcaag 540 agctcttcta ccaaaagctc ttcgtctctc aaggactcgt ctatcaagga cagatcggag 600 gtgacagttc cctccggcga gtcggactct tcagattcgg aaatggagtt ccccaaggtg 660 gtttccgaac ctcctagacc gtccactccc aataccacat ctcacgctga agatccagat 720 cagacacccg tggctcgggg cggccagaga agcccccatg tgaccatgga ctcgactgca 780 tccctgacaa cccccacgga ctacgagcgt gtgtaccgac gtcgaaatac cgccaacagc 840 acgcggtctc ggatgaccgt gcgagtggac actgccgatc tgcaatcgca gtcgcccaca 900 cccagcaacg gcatttccat cgactcgttt ctggagcaga aggagctgtc tttccggctg 960 gtcacctgga atgtgcacaa ccgggcccct cctgttattg aggacgagca tctcgattcg 1020 ctatttttgc cccagagcga cattactgtt cttgcgttgc aggaggctga tccggtcagt 1080 ggtcttgtaa gtactgctca gactctcaat ggatggaagg cggcggtgct ggagaccctc 1140 gaaaaggcca atgaggaaaa tcttcagaag gacgagactg tagtcaccgc cgaggaggtt 1200 gaagaggaga cggataatga taagacggag aatgataaga cggagaacga taagactgag 1260 aagagcgaca agtcggagtc aagtgacaag tctgacaaga aagacaagaa agacaagtcc 1320 gagaagactg acaaagctcc aaagactgag actcataaaa agtccgcacc ctccgacaca 1380 gaagaatctg gatacgaatc gtcggcctct ggatacgagt cgtcggcctc caacaatcac 1440 gcctactacg acccctactc caactatgtt gtttcgtcca accagctgat tggtctgctg 1500 atcattgtga ttgcccgcaa gtcgctcatg agccagattt ccgacgttcg agtcaagtca 1560 gctggaacag gtcttctggg agtgtgggga aacaagggag ctgttcttgt ggaaatgcat 1620 attggccgag aagccaccag cacgcagtct tccaagggcg acttctttgt gcccggaaca 1680 cgagtctgtt tcctcaactg tcatctttca gccggagaca acaacgttgt tcggcgacga 1740 tgggagattg accagatgta ccgacgcctg gagcttcctg ggcgtcccga atggtatgcc 1800 aaggtcaagg aaaacaagga cagcaacagc agcactgcct ctctggctac tgatgatgcc 1860 aggtacgccg attacaatgc atcaagcgag tttctgggca atgccacaga gtcttccgag 1920 agcgatgctg acgagaatga aagtcttcgt ggacctttca gtgcagctac cactatcacc 1980 gaaatgtctc ccaatctgaa cggacgagga ttcaacgata ccgaaagtgt ggtttcagct 2040 gattctcgtg cgggaggcgt ctttgactgg tctagtgatg ccgctgccgc cggtgcccag 2100 cgacctgctc gaccacatga aaactccaac ctcagtgtgg tcagtgaagc tgtttctgcc 2160 agctcacctc gaggcgaccc tcgaaccatc atcttcttcc tcggagatct caactaccga 2220 gtggacaaga ctcacgagga ggccctggag atgatcgaga agaacgattt cgccggcttg 2280 ctggctcacg accagcttct caaagatacc agagacggcc gtgttctcgc tggtctcaat 2340 gagtacccca tcaacttccc tcccacatac aagtacgttg aggataccgt ttccgagctg 2400 gacactctac gaactccttc ctacactgac cgaattctct cgcgagcctg gggaggctgt 2460 gagcttgttc agaagggata ccacagtcat atggagtaca ctgtcagtga tcacaagcct 2520 gtttcggccg agttttcgct atctctgcct ctgattgact ttgacaagcg cgctcagatt 2580 gtcaacaact atctcaagtc tgttggtgtt caggagaacc tcgaccggcc tgccgtcact 2640 gtttccccca tgagcctccg tgtccagctt gccgtgttga ccacccaaac tgttcctctg 2700 gtgatccaca atactggtca cactacagct cactgggaga tttcccagac ctctggattc 2760 gacgatgata gcaaggacaa gaaggactcg tcgcccagta tcaccctttc cagcaccacg 2820 ggccagcttc ttccgggtga ccaggaagtt gttgaggtca ccttctcggc tgccattgga 2880 gcgccctctt gcgactcgtt tgcaattgtg catgtcaagg actccaagga catttttgtc 2940 gacactggct acgatgtgat gccttcatgt ttcggctcag acctcgatta cctgtccaga 3000 ttgcctaacg gagctcgaaa cggtctgtca gacggcggaa aagtggtcag caacatgcct 3060 caggagattt ggaagtgtgt ggattacctg tggtctgttg tcgatcggga cggagaagga 3120 ggaggcgacg gagacagatc tgctcgtccc agtatcgaga agcctggaac agctgctgtg 3180 tctcgagagt ctcccaaatc acctttggtt ggcctcttca cgtcccctgg tgacccgcat 3240 ctggagatgg atattcgaga ttggcttgat accgggtctc cctttaacgt ggagactctt 3300 aacgacaacc ctctgggtcc acagtctgtg gcttctcagc tacatttgct gttggcttct 3360 ttgtcacaga gaatcattcc agagtatgcc tatctctctg tgcctgaaaa aaccatcgca 3420 gcaggcacgg gaagccgaat tccgtttatg ggaggaggag tggacaatac tgcggacgtg 3480 gctgcccata ttctggaggc tgtgcccaac gtgaatgcca acgtgatgat ttacatctgc 3540 tccttcatcc gtctgttggt agacagaaag gccatgactc tcaagacggc actggacgtg 3600 tttgcacctt tgctgatgga cagtcccaaa caaggaggcg tgtccaaggt gtggaagaag 3660 agtccaatca gttctgcaaa gctcctgcag cacatgatcg atacttatta g 3711 <210> 15 <211> 4245 <212> DNA <213> Artificial Sequence <220> <223> YALI0_A17776g <400> 15 atggcgatgc gcaaaaacat tgtgtttcac gagccgaaac gcgtatccga aacagaatgg 60 cagtaccccg agactggcag catatgggta catgtcccca ccggatggcg aagtcccgat 120 gaatttttgg actggtgcaa gcagctcaac gccaagttag acactactaa gcgatatcgc 180 gtgtcgcaat cagggctaac gcgcacattt tccgtgctat caatctgcgg tctaacaagt 240 cagagccagg cgttcatgcg gtctgaaagc ctgcgagtca ttgagattcc cgacgagatg 300 attgtggatg tgcggggcaa tcttctggag cccctgggaa tccgcaatcg cgatggcatc 360 acctatttga acgccgatac cagcacggaa agcatcacaa atacaactac cacagagcca 420 accacagccg ataccacaga tacaaccgcg gcctcaaaac cagaaaccac tgcagatggc 480 agttcaacga caagaagcac agctcgcgac aatgctgtgt cgaccgctgg aagcactaca 540 gagccgccca acacgacgac tgcggaaccg gccaccagct tctccaacaa tccaagcccg 600 ccagtgtccc tgctcagtcc gattcctcca gctgtagaga cgatcgcatc gtctacgccc 660 caacagagtg aaagtcccat tgtgcggtcg cctgttgtgc ggcccagtga cagtgcacca 720 gctgcttcta cagccgccac tatggccgaa atagttgctc ctcagcctgt aacaacggaa 780 tcctcatatt ctaaaccaac agaagccatc caggcgggat tccaggccat caatggcatt 840 ggaaactcgc tgttgacgtt tcaggccaac ccccaagtca gccagcctca gcagacacag 900 cagacacagc agcctcagca gacacagcag acacagcaga tacagcagcc acagcagcca 960 cagcagatac agcagccaca gcagccacag cagccacagc agccacagca gacacagccg 1020 ccaaagcaga cgccaccgac acaacagact cagtcgatac agccggcaca gccgacacag 1080 ccggcacagc cgacacaaca gttccaaacg acgtcaaatg cgagcaacaa ttgcggctac 1140 aagacgcttt ttctctgcat tccgtatcta agggccgatc gaaagctggg cctatcgaaa 1200 attacccaat cgcagcaggt ggttccctat ttccaatcgg tttcgcaatt tcgagaatgg 1260 gtccagaggc tcttccccac tcactacatg cctgctgaca tgtatggcat cactctcgat 1320 ccctcgcagt tgtcgggctc ggttgattcc atctccacca atcttatccc tatcacgcct 1380 gcgttccagg gttctcccca ctggtttatt tacagctatt cacctaattc gattgcgtca 1440 ccccaaaaca ttgccaatag cacacattct cgaactaatc ctgcgagaca cctcgcacct 1500 cagcaacaac aactgtttgg aatcactcag caaaactgga accggatggt ggacgtgtcg 1560 cgaaagcaca caaatattta tggcttgatg gataaacgac tgcaggcggt agagaaggag 1620 ctcgctagtc tcaaattcaa gctaggcgct ttggtttcac ttgagccccg tcaggaagcc 1680 gctgatcagc actgcatcaa actcggcaac cggatagatc agctccacag catgttgaac 1740 cagatgggtg cagaggtcac ccatactgtc aactctcttc agaatcgagt tgaatccgcc 1800 ctatctcttt ctggcaacaa caacaacaac aacaacaacc acgccactaa cgctaacaac 1860 gctgtggtta ctccagaaaa gcgcgcctat ccttttgaag aagaacactt gacgaaaaga 1920 cgccgaagcg agcattctaa taatattatg tttaactccg agaacgggtt tgttcctccg 1980 cccatctcgc gtatgttgag cgagttgaga aagtttccca cattgacgtg tgtaaatccc 2040 aactccaaga tcactgctcg gtttgcagtc atggatggca agtgtgtctt gttggcaggc 2100 tccgaggaca agtgggagtt tgggtttgac gacgacgatg tgggaggcag tgagccagtc 2160 cacacgtgtg acaacgcaaa cagttctgcc aaggcttctc ccgaaattac cactcaaacg 2220 agtatctcaa atccgagcgc aataatcgac gctggtaact ctgctcaggc ttcacccgtg 2280 gttcagacct tctcagtcgc acagtcctcg cctgttaatc ctgttaccca gttgcatcct 2340 tcggtacatg ccccacaact acgaggccct gtgagccctg cagcacgggg ttcatcgcct 2400 ctgcttgtct acactcatac tcatcagtct ccgaccgtgt cttctccttt agttcagtgt 2460 gtgcaccccc agtctccctc ggctcctgcc cctaccttga cgtccgctgc tgctgaaaac 2520 gtgacagtcc ctgcttccac tccggcaatt ccttcagtcc ctgtgggtgc cctttctcaa 2580 gcaaatgcca ctggtgggta cgtggaagta ccctctcccg ttgagaaggc cgcgcccact 2640 cttcctgcag ctgcgaacca ggagttgaac aacctgtcac ctgaacccgc cctggctccc 2700 gtcactgtgg agacccccac agctgaccgt gctagaagcg gctctgagcc tgtctctggg 2760 gccaagcaag tgacagcatc cgatcaagcc tctacagctg ctgcacatgg tcgtgtttct 2820 gagccggctg cttccgatcc tgcctctcaa gcagctgctt ctgaccgtac ttctaaggta 2880 tctgcctcac cagtggtgac ttccgcctca gacatgcata tgcgacgaaa cgaggcccgt 2940 aaacagcgcc agcagctttt ggagcaggag aagcgtatct ctgactctat cacggcgtcg 3000 ttcgctacca cagagaactc tcccgctatc gctcctactg caccacctcc tcgtcctcac 3060 ttctctcagt tggatgagcg cactctcgaa aggaacaggg aaagagccga agaggaacat 3120 ttggagcgag aagaacgtga gcgacgggag atggtacagc gagacgtgca aggacgtcgt 3180 aaggctgctc agaaagctca ggcccgtgcc aagtcccaac ttgcggatga acatgctgcc 3240 cgggaagctg cacacgaact tattcacgcc cgagttgctg ctgaaaggaa gcgtgctacc 3300 gttgaggagg ctgctaggaa agcgcgtctg caggccgaag ccgagagaca gagagaggaa 3360 gaggctgagg ctgaagcggc acggcagctg gagcagcggg tggtagaacg gcggaggaaa 3420 gaggttgaac aacaaaggaa ggagcaggag gccgaggctg agcttcgggg agttgctgag 3480 actgcaaagt ctgtgcaaga ggctagcagc aagctttcta ataggatcaa gaagacaaat 3540 caggctcgcg atgttgccgc agccataatc catcgtctga acggcaacaa ggacgatgaa 3600 gagagtggat cgcagtctgg tcgcgaacaa gaaacaactc cagtggcatc tgacgccttt 3660 tcccagtcag cttatccgca ggctactact gctgaatatg cttccagtgc taccgacact 3720 gcttcggctt ctacagggtc gtctatgaag gagcgagctg agaagatgcg ccagttgcga 3780 gacaagcaga aaaccatgca gcctatcaag acagaaatga actctgcgac tgacaatgag 3840 gccaatgata agtctgtcag atccagcaag ctgtccgaga agtctaccga aggacaaagg 3900 aagaagagga agagtgtgga cgagagcagc ggaaagcgag cggagactct gggaaggcat 3960 atcgaagacg ctcagaagcg tcctgacggc gtcaaaaagc gacccgacgt cactaagaag 4020 attgtatcca agaagggcat ctccaaacgc gctccttcca ctgccaaaat gagtatgttt 4080 gagcgtctga agaaaggcat tcctggcgag atcccgccac ctcctccacc gcctcctagg 4140 tcttctaaaa agggcaacaa gcgtaattcc aaggagaaga gtaaactcgc agtgtcttct 4200 gattcttcgt tgtcagatgc catctggatc gatatcagtg attaa 4245 <210> 16 <211> 3828 <212> DNA <213> Artificial Sequence <220> <223> YALI0_A17853g <400> 16 atgttgcgac tccaagtgta cgcaatttcg ccctgtctgg agtccgagac cgttactaca 60 tgggctccgg accggtccat gcagacgcga tctatcgacg aagcaggcat gcgggtggag 120 aaaaagttct tgcatttcac ggacccgcga tcttccttgc tggagctgtg ccacgagatt 180 gccgagcggt tcaagtccat ctaccctgac aactttgagc cccggatcga cttcctgcag 240 agcgacactg gcctcgacct gtcgcccagc tttgtgtctg gagacgtgtt caagtccgga 300 gatgtggtga gagcgattct gcggctgccc acagacattg aggagttccg caagtttcag 360 ggcgaggctc ctggcatccg atttctagag gaggatgtgg gagaggcagg agaggtgtct 420 aagatcgaag cacaggtcga tgatagcacc atgagcagag atggccgcaa gcgaagacga 480 tacacactgc ccaaagagat caccaagcga ctcaagcggg actcgtccgt ctactccgac 540 gggtttggcg tgcgtgatcc cgagtcgttc gaccacacca tggaagagga ggctatctac 600 gcccacacag tgactgttcg acgtcctcgt tcccgataca gccagggagg caccggaacc 660 ggccacaact ccattctgga gagttctact acctcgctga tcccccctcc ccggccagat 720 ttcgcctcgt cctcgacgac tggaccttct cctcctgctt ccaacaacca ttatcctcag 780 ttggagagca gccaggttgc tgccggatca tctacaccca tccgtctttc ttccaagctc 840 ggatccactg ttgttgataa gggaaatgtg tcaaacccca cccctgttcg aggcagcact 900 tctctcgata ccattcagac acccgttctg tcgccgcagc ccaaggcagc tatccagggt 960 tcccccatga agagccctcc ccacgcacag aaggcaattt ccttgcctcc tgtgaagaag 1020 gagcccacct caaaggagtc taaagacaag gagcctgttg ccaagcgtgc caagattgag 1080 ccaagggtcg agcgcaagcc cgagcctgag agcgacactt ctttcttggc tgagtttagt 1140 gacgatggca agtttcccac agctctcatg caggcccgtg ctcaacaaaa ggctgccatc 1200 gaggagagcc ggctcactca gcaggctctt cggcttcaca ttaagagccc ccagtaccat 1260 attccttctg agttgggcga ccacatttct cctgccaaag caaaggcttc tctgaacgcg 1320 tggttggggt ggaagcatgc ccaggagagt aactctcccg atattgagtt agctcgacac 1380 cgactggttc attgcacttt ttccgcactg gctgaggccg ccaacaagca gctcgacatt 1440 gccaacaaca aggtgcagaa ggggcagttg atggcctacc gtgagacttt cagcgtgcgg 1500 gctgataaca gctacaagtg tctgacggcg gagcaagttg ctgctgagaa gctggctgct 1560 gatgaggagg tcgccaagga ggttgctcga attaaggcta gacaggtgag caaggatgcc 1620 cgggatacca agcgtcgtgt ccgtcgggag tccacagctg ctgctaatgc ccatgaggcg 1680 gccgtgatgg ctgccaagga agagcatgac cgtgcagagg cgatgcgtgc aagagaggca 1740 aaggttcgag agcaggaggg tgccgaggcg cgcaaggaga tgatcgagga cgagaaggca 1800 cgtcagctga agttgaagaa ggaggaggag gccaagaaga ggaaggagga ggaggccaag 1860 aagaggaagg aggaggaggc caagaagaag aaggaggagg aggccaagaa gaaggaggag 1920 gaggaggcta agaagaagaa ggaggaggag gctaagaaga aggaggagga ggccaagaag 1980 aagaaggagg aggaggctaa gaagaaggag gaggctaaga agaaggagga ggctaagaag 2040 aaggaggagg ctaagaagaa ggaggaagag gctaaactgc tcgagctgaa gaagaaggag 2100 gaggctaaga agaaggagga ggctaagaag aaggaggaag aggctaaact gctcgagctg 2160 aagaagaagg aggaggctgt ccgggcagct gaggaagcca agcgcaagga ggaggctaaa 2220 ctcaaggacg ctgaggccaa ggaaaaggct gccaaagagg ctgccaaaaa gctggaggtg 2280 gagatcaagg aaaaagctgc tcaagcggct aaggggtccg ccaaagctga ggcggacaag 2340 aagaagattg aggaggcaga gaaggccaag caacttgagg ctgaagaagc gcgagaggct 2400 cgagcccgac tagctcagga gcaacgagaa gccaaagcca aggccgctca ggaggcaaag 2460 gaggccaggg aagcgaagaa ggccgagcag caagccgctc gggaggctaa gctccgagca 2520 gccgcagagg ctaaagaggc gagagcgcga gcagccgcag aagctaaaga agccaagctc 2580 cgggctgcag cagaggccaa agaagccaag gcccgagcag cagcagaagc acgtgaggca 2640 aagaagatgg ctgaagaagc aaaggccaag aaaaacgccg aagctgaggc gaagcagaaa 2700 gctgctgagt caaagactga ggtcaagaaa cgaacccttg gtgctgctaa agccgcgcct 2760 atttcttccc aggacacgca tgtttccgac tctcaggctg aggacaagat ggatgtcgac 2820 tctgattccg actctgagtc tgattctgat tctgattctg actccgactc tgattctgat 2880 tccgattctg actctgactc tgactctgac tctgactctg actctgactc tgactctgac 2940 tctgactctg actctgacaa cgggcccaaa cccgagatca aggcgaagcc ggcagccaag 3000 aagagttcac agatgcctcc ttccacccaa cctcctccca aagtgaagca gactgctccc 3060 aagtcttccc agcctactcc tgccaagaag gcaatgaagg aggagtctga gtctgagtct 3120 gattccgatt cggatgatga tgactctgat gacagtgact ctgacagcga ggacacctcc 3180 aagaagagaa ccattgtcaa gactcctgcc aagaacgcga aggctactac caagcctgag 3240 ccgaagactg ctgcgaagcc tgcttctaag actgaggcta agcctgcttc taagactgag 3300 gctaagcctg cttctaagac tggggccaag cccgcttcta aggctgagac caagcccact 3360 gtcaagactg aaccaaagac ggctgtcatg tctgagccag caaagactgc tcccaaggct 3420 actgcttcca agtcttccac tacagtgaag cccgaggcca acggctctgc tatcaaaaag 3480 gaaggcgagg ctcccaagaa gtctcctttc tccaatctca agtctctcca gtctttggcc 3540 aagatgggtg ttcctgaggt gaaggactcc attccttcca gccagccccg attctctcaa 3600 gcctcccaga agaagaccga aaaggaggag gagtccgact ccgatgatga ctccgacgac 3660 tccgacgact ccgacgactc ttctgacgat tctgatgatt cggacgattc tgatgactct 3720 gatgactctg acgagcagcc tgccgccaag actaagcgtg ctggagctgg gctcaagaaa 3780 aagaagaaga acagtgggtt tgccagcctc atgaacaagt ttatgtaa 3828 <210> 17 <211> 1908 <212> DNA <213> Artificial Sequence <220> <223> YALI0_A19646g <400> 17 atggtcgcct acacggagcg actggcgtcc tttgaagaag cccgacttcc tcggcggaga 60 aagaaggtcc aatggcctca tgaacacccg gatccagagc agttggccaa ggcaggattc 120 tatttcaacc ccagagtcga gagtccagac aatgtcacat gtttcttgtg tgaatgttcg 180 ctggacggct gggagctcga cgactgtcct ctcaaagagc atctggagca ctctcggggg 240 tgttcttggg ccaccattct gtccaaggac tggcagaatg aaaagaatca cgaccctcac 300 tgcaaagaga acataggcat gcgactcacc acctttgaca acaaatggcc tctggagaag 360 aagcggggct ggccaacgtc gctcaaactg gccgaggcag gcttctactt tgcgcctacg 420 gtagccgaag aggatctggt tgtgtgcgcc tactgcgaca tttcccttga cggatgggaa 480 agaacagatg accctcttca tgagcatgag cgacgacgtc ccgagtgcta ctttttcacc 540 agcatgaaga aggaggaggc tactaccaag aagaaacgaa gatcttctaa gcgagcttcc 600 aagaagattg aagaagtcgc cgatgagagt gttgtcattt tacagcagcc tgacaatggg 660 agtgttcaga ttctgtcgga tcccgaagag gagccagccc cccagcccat tagatccaag 720 aaggagggcc gacgaccaag cgcaaagact gctccgaaga tcagacaggt gtctgtagaa 780 gaagagcttg ccaagctgca gaaggaaatg gaggaggatg gagacatcaa tgttgccttg 840 gagcaaaagg aggatgtttc tgactttgag gttgaccttg agctcagcga gatggcaaaa 900 caggccgatt ctctggtcca taactttgat gaaggtatca gcgactttga ggaacctcac 960 cacaaccacc agcatgagcc tactcctgtc agatccaaca agcgaccttc taatgtcttc 1020 caggatgatc ctgcacctcc tgccaagcga cgaaagtctt ctgtggttcc ttctgcacca 1080 gttgctcgaa tttctctgcc tatttccgag ggtaccaata attgggaggt tttctctgga 1140 tcgtcttctc cagctcctca agagcacgaa gtggacaaag gagaagacgg cggtgatgac 1200 aatgatgatg aggaagacga cgacgacgac catgaggacg atgaggacga tggggacgat 1260 gaggacgatg aggatgagga ccagttcgtg gaggctaagg aaaacctcac cgaggaggac 1320 tgtgtggttg tttctcctgc caagcctctt gagaagattc ttgccaacga cgtgcaagct 1380 catgtggaac atgaagttga ggagcccgct cgtgaggaac aagaaaagga gcaagttccg 1440 gttcaggagg aggaggagga gggggaggaa ggaaaacggg aagaacctgt tcaggaggaa 1500 gcccagtcaa acaaaactac agatacccat agcactcctg ctgactctga gaggcgagac 1560 tctgagcgtc ggtcgagggc aatcacccga tctcctcttg ctgttcgcaa cctcaaccaa 1620 atcgttacca ccaaatccgc caccagcact ccagcacggt ccaaagcgag ccagtctcta 1680 caatgggagc ctgtggactg cgaccaggtc tttgacgtgt gtgagtcgag tcctcataag 1740 gagcctgatc acatcctcga catgacaatc acggagtggt acaagtacca gtcagtagag 1800 gccgagaagc ggctcatgga aaagtgcaac cgaatggtcg aggttgtgca gcgagaaggc 1860 cagagggcgc tgaaccatat caaggctctt cccacagttg atgggtag 1908 <210> 18 <211> 996 <212> DNA <213> Artificial Sequence <220> <223> YALI0_B00748g <400> 18 atgatacccc caattacttt tccctactac catgaaatct ccgatcgata tggacttgtc 60 ttcccagaca gcacgccctc aaacggtctc catgacaatt tcatcaatcc actactcaag 120 gactggcaca cagacaatgt cactgttctc tggttgattt tgaggggaat ggacacccca 180 tccctcaagt cccacgacga tattgaagcc ttccagatgg atagcgaacg gatctgcctt 240 cgcatcttta acaactacta tgagctgttt ccaacctcag agagccttgt aaaggcgcag 300 accccacatt ccaatggatc ccgattccaa tccctcaagc tgttatctca aatgaaactg 360 gaccgacaga atccctgcag cgtggtcaaa tacacagccc agctcgagtc gtccgcaatg 420 tactccaggg tactcaagtt gttgctcaaa gacaaaactt gcgattttac catcacatcc 480 accacgccca cgtgctccga ctccggctcc gactccgact ccgaccccga ctccgactcc 540 ttccccgtgc actcgttctt actcaccaac ctctggccct ttttcaaagc agtctcgtct 600 gcggagatgg ttgaaaagga gacccaaaca ctccatctac cgtttccgaa ggactgtgtg 660 gaaattctcg tcgccttctt ttacggaaat aacgtccaaa atacctcctg gagcttatcg 720 ctgtctctgt ctctgctgga aatgtctgcg ctctacgaca ttccggagct gaaaaatatt 780 gccatagagt caattgtcag cagtgctgaa ccgttgtctc tctctgtcgc tctcaaagcg 840 tggaaagtgg ccaacgaaag tgacgctcct gaagtggagg cgtttctagg gccgtatctg 900 aaaagacacg tctccgaaat tgaagcctcg gaggaatcag aggagttttc cgaatcacaa 960 ctgctgcaat tactcctgca gattgtcagg ttgtag 996 <210> 19 <211> 1669 <212> DNA <213> Artificial Sequence <220> <223> YALI0_B08800g <400> 19 atggagggcg tgggagtgtg gagagagggc cccgggttgt gctacacaaa gacgacgttt 60 ggaagcagtg tccggagaat atataagggt gacgtttcgc acattaggtg tggtggcggt 120 acagtagtgg tgtgtgtgtg tgtgtgtgtg tcgttgcctc agtcactcgc ccacttgctc 180 acctgtactt gtcagtagct tgttggttgg tttcgcgctt gcgccctttg atcgcgtacc 240 caataaggct cacctgcatt tgcgccaatt ctaaactgtg gaggcaaaaa ggctaacctt 300 cggcatgaca ctacaggaat ctgcaacagg ctagatatcg ggacaagacc agatacgtct 360 ttgacctcgt ttccccgtca aaaattgcat gtccgacaac gagaagctgg ccgcggcgat 420 gcgcacgcgc ctcaacttcg ccatggtcaa ggtccagaag ggctgggagg accggtccat 480 tgaccagata gaggaggcga ctaaccacca ggaccaggcc aaggacaaga tacacacaca 540 cacagacaag aagggcgcac agatacagac acagtcacag acacagtcac agacgcagtc 600 acagacacag acagacaaga agggcgcaca cacacaaccc ccccgcaaga cccacgcacg 660 cagcgacatc tccgccgcag caataggtcg cacggtgggg ggcacaagtc cggtcaagga 720 gcaggcggct tcatcttctg ctggagtgcg ttcggcagac tcgtctccaa gggtctcaca 780 ggcctcattt gcagtgccgc agacggccag taccggcctg ggactgtcaa taccgacctc 840 tccgacgcca cgaaggcggc cctccaagcg gggacaccga cgctcgacct cggacattct 900 acacaactcc actggtggag tgtccggcgt cttcaaggtc gacaagcccc aggagagggc 960 gtggggcact ccggcgcagc caaagcagtc accccagaag taccacgccc agattcagca 1020 cagctacccg cccaactctt acccgccaaa cacctaccct cagaacactt actacccccc 1080 tcctccccca ccacaacagc atagtggtat ccactaccct ccacagcact accctcctcc 1140 tcctccgccg cctcccccgc cacaagatca cggatctcct acagtcacac cttcaggcat 1200 ggtgcaccac tccccaccgc acgccttggg ccaggccttc caaccccagc ctccacagcc 1260 gtccccccaa catcaaagac agtacaatta cacccacgcc ccgccctacg gatcgccacc 1320 tcaatacagt caccagtcgc actcgccaac acgcccgtca ccgctgcatg gaccgccaca 1380 gacgcctccg acgtccaatc ccacgtcagc atcgggaacc agacccacgc tggcgcctct 1440 acacgtgtcg ctgccgccga tatccacgtc catcaactcg ccaacgtctg cgcctgacag 1500 agtcaccctg ccgcccatgc gtgctatcta cgagccggtg atgccgtatg gggacgttac 1560 gagtccccga gcgcatcttc cctctcccgc gcctcattca ccgggccaga gagaggtcga 1620 tgacgatgct gcagagtcgt tgatgtattt cagctctccg agaaggtaa 1669 <210> 20 <211> 1544 <212> DNA <213> Artificial Sequence <220> <223> YALI0_C06424g <400> 20 atgggactcg ctaacatcat caaccgtgga gaaaagcccg agggctcggc cttcatggcg 60 gcctttgtgg ccgtgtttgt cgcgtttgga ggtattctgt ttggatacga cactggaacc 120 atttccggcg tcatggccat gccattcgtc aagaagacct ttacagatga cggcctggag 180 ttcacttctg agcagacctc gctcatcact tccattcttt ctgcaggcac cttcactgga 240 gccatttctg ctccctgggc ctctgatact ctgggaagac gactgggtct gatcctcttc 300 tgtgtcgtct tctctgttgg cgctattctt cagactgctg ccaccggccg aacgcttttg 360 attgtcggac gagttgttgc tggtcttggt gttggtggag tctcttccat cgttcctctt 420 taccagtctg aggttgcccc caagtggatc cgaggtgccg ttgtctccat ctaccagttt 480 gccatcacca ttggtcttct gctggctgcc attgtcaaca acgcaaccaa aaacaaagac 540 aacagtgctt cctaccgaat tcctctcggc cttcagcttc tgtgggccgt catcctgagt 600 ggaggtctca tcctgctacc ggagactcct cgattctgga tcaagaaggg cgagtacgac 660 aaggccgccg attccctgcg acgactacga cgacttcctg ttgagcacga ggctgtacag 720 aaggagctcc tggagatcca atcttctcac gaccacgaga tgcagatcgg tagcgccacc 780 tgggccgcct gcttctcccc caaggggtcc cagctgaagc gaatgctgac cggtattgcc 840 attcaggccc tgcagcagct caccggtatc aacttcatct tctactacgg aaccgagttc 900 ttcaagaagt ccaacatctc caaccccttc ctcatccaga tgatcaccaa cattgtcaac 960 gtggttatga ccatccccgg tatcatgttt gttgatcgag tcggacgacg aaagctgctg 1020 ttgatcggag ctatcgtcat gtgctcttcc gagtttatcg tggcggctgt tggtactgcc 1080 attgataacg agacctcctc aaaggttctg attgccttca cttgtacctt cattgccggt 1140 ttcgccgcca cctggggtcc tattgcctgg gttgtcattg gagagatttt ccctctacga 1200 atccgagcca agggtgttgc tctatgcgcc gcctccaact ggcttttcaa ctttgccatt 1260 gcctttgcaa ccccctacct cgtcgacgag gcccctggat cggccggtct caagaccaag 1320 gtcttcttca tctggggagg ctgcaacttc ctgtgcatcg ccttcacttc ttcatctacg 1380 agaccaaggg tcttactctg gaggaggtgg accagatgta cgccgagatc aagattgctt 1440 ctcgatccca ccagtttgtg cctaccactc gagtcgctgc ttacgacgag cacgcttctg 1500 acgacaagaa ggacggacag cacgtctaca ttgagtctgt ctag 1544 <210> 21 <211> 2481 <212> DNA <213> Artificial Sequence <220> <223> YALI0_C07172g <400> 21 atggcccgaa aatccacccc agagcctgag atgagacgca agactcgggt caaggccaac 60 attttgcatg tgagcaagga agggatattc gacagcgccg gaaagtctgc tcagatcttt 120 gacaatgcgg gaaccaagcc tgggtcccag gcacagttac gtcaagagca acaacaagag 180 caacagacac aaacacaatc gcccatggca aagctgaagc aaatggtacg ccgaaacagt 240 gctcctcagc agccccagtt gcagcaaccc caccagtcac agcagcagca acagcagcag 300 cagcagcagc agcgacaaca gggtcaaccc caacaacaaa cactaaccac ccgtctgtat 360 ctccaaaaca agtctctggc acgacagaat tccaatctca acaccaaaat gtcggctttg 420 gaacaccata tgagcgaact tatcagtgaa aacatttgtt tgcggcaaaa gaatctcgag 480 ttgcagagaa gccacgacaa ttggttgcga tcgcatgtca gccagaatct caaaaagcaa 540 ctgcaacagc atatcaatgg aatcaacgat ttgatgcaag gactgttggg agaagtcgat 600 ggagactcgg agcaacgctc tcatgaatcg gcttctcagg agcactttga gctttccctt 660 tccagatcac tttcaaagac tcccgattct cgagctctat ctagaggttc tcctgtatct 720 ggcagacact cgctctctag aggttcggca agtcgacaga ccatgggtcc tgctgacttt 780 gcacgacgac ggtcttctcg acgtcgatca tcgttctttg gcgagctgcc cctgctgcag 840 gacgccgagg acgtgctcga tccttcggca gaacaattgc atttggaggc tcgagatgtc 900 gtcactccga tggctgacga cgttgaggga tggggtgagg ttccagttga cgaggtgaat 960 gctcacatgg aggagatgga gaagcagcat caggcagagg aggcagagag cgagatggat 1020 gttgatagag gagagaaggt gcatttcaat gagcacgtgg aggaacatta cgacgagcca 1080 atggaaatgg atactcagga gacacaggat acatcattct cctctgctgt ccgggagact 1140 tctttccctt cacctatcca agagactgtt caggagactt ctttcgcctc tcccgagcac 1200 gaatcgttcc cttccctgat ccaggagact tctttcccct cgcctgtgaa ggagaagtcg 1260 tcgtttgctt cacctataaa agagaacgcc tcttttgcat cgcctgccaa acagaagccc 1320 ttcccttcac caatcgacga gtcttctttc ccgtctccct tctccagctc cttccgacgg 1380 gtaacttctg cccccagagg agagcctatg gaggaagtgt ccacagatgg agagtccttt 1440 gcattccttc agcagcgatc tcgtcccaac tcgagccatt cacggcctaa ctctcgatcc 1500 gcttctcgca acatttcctc ccgccctgga tctcgggaga agcagatcca cgtgtttgcc 1560 gaccctgtat cccctgttaa ggaagctggc cttgaggcct ctgcccctgt gaacccagcc 1620 ccttcaaata aaccagctgc tgtctctaca acccctcctc gatctcgtcc ttcattgttt 1680 gatacccccg agagtgccaa ggagcgacag gagaaggagc ggaagcgacg agagagcatg 1740 gagcaggtag tggaggatcc cgtgtcaccc tgttcccgtc ccagcagtcg acgatcttcc 1800 cgaagagcca gtactgagaa ggagaaggcg gctgaggagg ctgcaaagca tgaggagatg 1860 gaagttgagg aagagcatgt tcaggaggag aaggttgaaa ggagggttaa tcgttctcga 1920 cggacttcta ctaagacagc tgagtcactt cctgtatcta caggactcac gactgtggag 1980 tctttggaga agcccgtttc gggaatgttt gaaaagggtc tcgagaagct tcagagtctg 2040 gacaacaacg tgactgcccg gaagagtcgc gctccaactg ccaccggtgg tgtcaaggtg 2100 actacatcaa gaaagagcac caaggccact gccaaacctt ccaagacctc tcctctgatg 2160 cagaaggatg ccaatggcag caaaaataat ggagccgatg gagtcaaggg caaggtcgag 2220 actgttaaaa catccaagct tgccgaaaag cccgtcaagc ccgtcaaggt ggcagctgat 2280 acggttgcca agaagcccat caagctggaa acccctgagc ctcgttctcg gcgatctcga 2340 ggggctcctg tcaactacaa gcttccctct gtgggttcca agctgcgtcg ggagaccgat 2400 ggctttgttg atgcagttgg agggctcaag cggtctctgg gtgcctgtga tgagaacggc 2460 atgaagcgac ggcgcatcta g 2481 <210> 22 <211> 1797 <212> DNA <213> Artificial Sequence <220> <223> YALI0_C08437g <400> 22 atgactcgat ttactgccag gcagggcatc aaggcgccgc ggaagctggg aaacgctcag 60 agcggcgccg tattggccgc gcggaaacgg caacggcaag aaatggactt tctgaaggag 120 atgcggcagg ttatggaccg agatcatcag ggtccctcgg cggcggactg cgttcgagtt 180 agagaaggaa agaagcagcg ggaggcagat cggttcaaag cggacaatca gacggtgcgg 240 taccgtctgc ctaccaagtc ttgtgactac cagcgcagta tcgatgaggt tgtgagcggg 300 ttggctgctc gagtcgattt ttcgcgggag ggacgacgtt tgtcctttct gcgtcgaccc 360 gcggccccag aggttggaag ttcagattac gaggcgtttg aagcgacacg aaacaacttt 420 tatgctcaga tgcttgcgac tccccccaga aagggcgttt tgggtgacat tctacttcct 480 cgagggatgc tggatcagtt tgcctccatt aagagtgatt ctggggtgga cagagacggc 540 gaaccgactg tggcacacgt ggttccgatc attgaaggca agcctgtgat ccccaaggag 600 atggagggcg tttcagacct agcaatttct tccaaatggt accagagagc atgtcttctt 660 cgattccagg ctgcttttgg cacagatgat cgtcgcttgc atggcacatc tgtgcgtgtg 720 atgcagcaac agggccgaaa ccggatcttc gaactagaga ctttgttgga gatagctcta 780 gctgaaagac aaagactagt cgagaaggtg gaggaggttt tgaaagctgg gtataaggat 840 ctggacagca agagtgtgac agagaaaggc gctattgtct tcgactttga ttccaactcg 900 ttcgaaccat tccagtatga aaatggagct gtgagaagga tctacttctc tgtctgtggc 960 atgggtgctt ctgaatttgg actggcagga aacgtgtcgg ttcctatgag tgagtctatc 1020 aacaagctca tgtgggaact gttccgacac tctcttcgag ttagaggggt tggggagatg 1080 agttctgatg ctctgcagcg gatccaggct cgaaaacagg ccattatcga ctacaacaac 1140 ggcaaatcca agagaaacgt catcaatgag cgcatgtttg acgcaggaga gaagaggtca 1200 cttgaggagg aactcatcag ggatattgct ctgcttgatc ctcaacccaa gggcaaggtc 1260 aagaaagacg ctgtgaaggt tgtcaatgga ggtgccaaga gggatacccc tagtagggcg 1320 cctaaagaga cagaaaaagg tgataccagg ggttctagag atggaagtgc gtcgaatgga 1380 aagggttcca acactaggga aatgaaggct tccaacgact cgaacactag aggttctaga 1440 ggtgtccagg agatcaagac tcagactagc gacaccaaga gagaggttga tgagtttctt 1500 accattgaag acctcgtcga accttccggc aagaaatcca acaagaaatc caacaagaca 1560 gccagcaaga cagccagcaa gacagcgaaa aagacagtca gcacgacagt cagcaagaca 1620 gtcagcaaga cagtcagcaa gacagacaag cctcttagca agccttctaa taagtctgca 1680 tcggccactg tggaagacac gttccttgac atggactttt tgctagaaga gaaacccaag 1740 aagcgaaagg agtcaaagtc cacgaaaaag aggccgcgca agggtaagaa gttctag 1797 <210> 23 <211> 4143 <212> DNA <213> Artificial Sequence <220> <223> YALI0_C09031g <400> 23 atgcgattct ttcagtctat tgccctgcta gccactctgg cgttcgccaa ggaggtcgct 60 tcgcccatca catccttcga ggttggacag gctaacgaca tatccaacaa ggagggatac 120 accggtagcg cttgggagct ggactacaag tttgccctgt ctcctgaggc cgacaacgtg 180 cagcctggtg atcacttcac gtttgatatc gatgacaaca tgtctcttga cgctggaaac 240 tacgacttca tggtcaagga tcccaatggc aacgatatca tgcgaatcac taatgaaggc 300 catcacttta ccgctaccta caccgatttt gttgcttcca agaactacca gattactggt 360 aagttcttca tgcagtccac tctcaacagc cagtatgtca agggccccgg tcccgtcacc 420 atcacttctt cttccggagg caagcagttc tctgatcaga tcgaggtcaa ggctgctgtc 480 gacgccggtg gagcttacaa gtacgcccag cgagtcggta accgaatcga atggaccatc 540 caggttcctg cttctcccta cgaccgaatg aagattgttg atcagcttgc tgatgccggc 600 tctacctaca ccacccccga tgctcttctc gaggatatga ctcttcactt ctaccacggt 660 ctgaccccca cttgggacta cacctcctac gagaagatca ccgatgtgtc ccagtacgtt 720 cagctccagg acttccagac cgacgggttc accatgttcc ttagcaacat ccccgaggat 780 gatgtcaccg tccagatcac cttctactcc gagatcacct ccaagcagga gcagtactcc 840 aacaacatgg actggctcat gtgggagcga ggctacggct atcttcccga gccctctgga 900 gagcacaacg atggttcttc cggcgagggt aacgccaacg tcaacaacgg cattttccga 960 gctggccatg cttacggtgg tgctccttac gatgctccta ccggcggcaa cgatctgaac 1020 gctggtggtg agggtgacgg tgagggacga accaagacct cttctgttga gcccaccact 1080 tctgctgagc ctacttctgc tgagcccacc tccgagaccc gaacccccgt gtccgagtac 1140 atctccaagt ccaaggcacc tggtgagtat cacaacaact actactaaca aacccaccaa 1200 cacatactaa caataggcaa ccctggcgac ggatttgttg gatcccgacc caccccttct 1260 acctatggtc ctggaggccc tcctcctgct tctgctggtc ctgagctttc cactgacaag 1320 acttcttcgg ttgagcctac ttcttctgct gatcctacta cctccgagga gcccacttcc 1380 tcagttgagc ctactacctc tgaggagccc tcttccgttg agacctcttc tgctgagcct 1440 aattcttctg ctgatcctac tacctccgag gagccctctt cttcagttga gcctactacc 1500 tccgaggagc cctcttccgt tgagacctct tctgctgagc ctactacctc tgctgatcct 1560 acttcttctg ctgatcctac ttcttctgct gatcctacta cctccgagga gcccacttct 1620 tcagttgagc ctactacctc cgaggagccc acttcttcag ttgagcctac tacctctgag 1680 gagccctctt ccgttgagac ctcttctact gagcctatta cctctgctga acctgctacc 1740 tctgaggagc ccacctcttc agtcgagccc acttcttctg ttcctgagtc tcccgagtcc 1800 tctgctgagc ctaccagcga agagacttct tccgctacct ccgaggagtc ttcttccgtt 1860 gagcccactc ccgagcctac ttcttccgtc gagcccactt ctgaggtttc ttctgctccc 1920 gagtctaccg agatctcttc cactgctccc aaatctacta ttgaggcccc tgttgttgcc 1980 aacaccaccg tctcttccac tctcgagccc acctcctcca tggctgagtc tactgttgag 2040 tccactgtcg agtccactgt tgaatctact actgaggtct cccccactcc cgagcccact 2100 tcttccgctc ccgagtccac ctccgagtct ccctcttccg ttgagtcttc ttccgtgttc 2160 tccaacacta ctaccgtttc ttccgccgct gtctcctccg ccgctgtttc ctccactcct 2220 tccgtgtctc tcgtctcttc tacccctgtt tccattgaca cctgcatcga gaccgagact 2280 gtcaccgtga atggcactgt ctcgactgag accaaggatg tgtgcgctac ctcttctacc 2340 cctgctcccg tgtctaccaa cacctgcatt gaggttgaga ccaccaccgt ttctggaacc 2400 gcctctaccg ttaccaagga cgtttgtgct tctaccactc catctgccga cccctgcact 2460 gaggttgtta ctgttttgtc caacggtacc accactgtgg ttaccaagga cgtctgtgcc 2520 tccaccaccg tttcggctga tccctgcact tcaaccgagg ttgtgtctgt ctccggtgtt 2580 gtctccacca tcaccaagga tatctgcgct tctactactt cttcggatgc ctgcattgag 2640 gtcgagactg ccactgtcga cggctctgtc accacggttt ccaagaacgt gtgtgaccct 2700 gtcgtgactt ctgacgcctg tgttgtcacc gagactatct cttccaacgg ctccatctcc 2760 accgttacca aggacatctg cgcttctgcc acccctgtac ccactgtcac tgactcttgc 2820 acccaggtcg ataccgtgac tgtgtctgga accgagtctg ttgtcaccaa ggacttgtgc 2880 tcttctgctc ctgtttccac cgactcgtgt tttgtcacca aggttgtatc taaggacgga 2940 gctgtttcca ctgtgaccaa ggatacctgc gcccccgctt ccactgactt ttgcactgtg 3000 gttgagactg ccactgtttc cggaactgaa actgttgtga ccaaggacgt gtgtgcttct 3060 gcaactatca ccgccactcc ttcttctgac gcttgcattg aggtttccac caccaccatc 3120 tccggtgttg cctctacagt caccaaggat gtgtgtgctc ctctctccac cgactcctgc 3180 attgttcgag atactgtcac cgcctccgga ctcgagactg tggttgccaa gaacacctgt 3240 gtcggaacca tcaccaagac cgtcacccac tgcgatggtg gttgcactga gctccccgtc 3300 actttgactg aggcccccgt ctcctccacc gccgttgtca ccacggtcgt tgagggcaag 3360 accgtcactg tcactgtttg cgacgaggat ttcaagacca atgagtctgt caccgtctct 3420 ggctctgctg ctgagattac ctccaccgtg gttgtaccca tgaccatcac caagactatc 3480 accaactgcg acaacggctg ctccgagatc cccgtcacta ttgtcgagac ccccgttgcc 3540 tccaccgagg tgatcaccac cgtgattgaa aacaagactc tcactgtgac tgtctgcaac 3600 gaggacttca ccactgaggc cgttgagact cctactcaga ctccagccca gtctacccct 3660 gttgtcactg tcgtctcttc ttctcccgcc gagtctcccg ctgtctccaa gattgtcaag 3720 accgtcactg tctgcaacgg cgctggctgc tccgaggccc agtctactga gaccgttact 3780 ctgcagaccg tccagactca atccacagag attgtctctg cccagcccac tcagtctgtg 3840 gccatcgagt ccaaggttga gtctcgagcc tcgactacta gcatcaccgt tgtcgagact 3900 tcccccgaga cttccacacc tgctcagccc aaggagtcta ctcctgctca gcccaaggag 3960 tctacccccg ctcaggagtc tacccccgct cagtcctcta ctgaggttcc cacacaggct 4020 cctcagcagt cttctcctgc tcctcagtgg cccgatgccc ctaaccccga gcaggccaac 4080 tctgcttcca ccaaggtgat tggagctctt gctctcctcc ccctctttgc attcctcatt 4140 taa 4143 <210> 24 <211> 2637 <212> DNA <213> Artificial Sequence <220> <223> YALI0_C09614g <400> 24 atggacctga gactggtacc gaccagccgc gcgcgggtca aacggctgat ctcacgtgag 60 ccgtcgccgc tgcaactgca gcaaaaacga aaggacgaat cgagcttcgc gctcgaggag 120 atgcttagtc agcgccctcc gaccgacgtg cgctcgattc tcaactcggc ggtcgacgcg 180 ctgaggaaaa agccggaggc ggcggcggca gccgggaagt cacgtgagct ccagacgggc 240 tcgaccctgt ttgtggaaat tcagccggcg ctgattaaca agctgcaagt ttcgacgcag 300 tttttgctgg tgaagattga tgatggcgaa ttctggatgg agcggtttgg cggcgacatt 360 tggggggttt tcgagcgaag cgcggcacgt gataagcaga ccggatcccg tggccagctg 420 gactctcgac cccgtgacat ttcacatgac gacacggagg acacccagga ggacactctg 480 ccagatggtc aacaagacac ccaagaagat acaccaagac aggatattag tccacacaaa 540 ttgttggccc acaaaacatc tacaagtcac gtgttagaac cgaaccgttc tcgcatgcat 600 tcttcctcac gtgatgatat tctttcccgt gctcctgttg cctctagcaa gtcctcttcc 660 actactaata ccactaccca ggatggttca ttcacatctc taattgcatc tctaccacct 720 cctcaaccga cgttaacccc ctacattcct ctaaacctga cgaaaccagt ttcggcgcta 780 ccattgccat cagtttcggt ctctgagctc cttctgtcgt cctaccggac ccagctttac 840 tgctgtcgga cgtcgctcat ctactttgcc aaggccatgt ccaagtcacg tgctctgtgc 900 aagtccacgc tcgagaaaca tacgcagggc aagtctcgtg ttttttccgc ctcccagatg 960 cgcaaaatgt cgtataagct gtactctcgc gagttggcca agctcgtcca gccactcaat 1020 gaatggtctc aggtttacga gtaccacgag ttctgcaaga tgatgacaaa cacaaaagac 1080 gagtacgtga tccagtggaa agacagactg tcttattcgg ttttgtccga ttcggaaaaa 1140 ctcgcagtgg aaatatctct tctcaagttc agagaaatgc aattacagag tattgttctg 1200 ttggaactgc tggcagtggc tccggtgacc aaaagtgctg acgctgctga gacagtcaaa 1260 cagatggagt tggaaaagca aaagaagaag gaagcaagga aaatgggcct ctacaagtcc 1320 agacgaaaca aggcagcggt ggaacaggag gaggctgcag aggagactct ggactacaag 1380 actctgttga tccagttgtt ttccagcatg tgcatctggc aacaattgtc ggattcaaaa 1440 tctgatcacg tgcacgagtt ttgcaagtcc gtggtcattc cgtatttctc gtcacgtgtt 1500 ccggaggtgg tcgaggactt gctgaagaga gcaggcctcg agagagaaac tagacacatt 1560 tctggtaagc aacaacaaca acaacaacaa caacaacaac aacaacaaca acaacaacag 1620 ccgctgaagc agaacaacaa gccgcgagag atcccatatg catcgcgcga ctcgcctacc 1680 ttgtcacgtg actcaaccgt gacaccgcgg gccaactctc cgtctttttc acgtgattcc 1740 gacacgttga attcggacat cagcacgagt tcaggtgtgt ctcgggtaac caattctagc 1800 cgcgcagctc gaacaggctc ggcccaggga gtcacgggat tacttaataa gccgcaggtt 1860 cggggcggac tcctcacctc ttcacggtca tttgattctc gtactcaggt gggcatgcgg 1920 ttcaagggca gtaaagagaa ggacaaggag gaggtgaagt gtacaaattc ggttgccggg 1980 accacaacgt cggctacagc ggagatgacg acgggcatgt tcagaaagtc acgtgacatc 2040 tccgtggcca cagcctcttc tcgtcattcg atggcggccc agtctcaggt ggctgccact 2100 cccgtgaagc ggactagacc ggttttgacc tcatttacag ccgagccgga caatccattc 2160 atcaacgaca gtcacgtggt tgacgagtct ccgtttgcca agcggatgag aggggagagc 2220 actggtccca ggagaaggct gggcctgggg gatggcactt ctgttgtttt cgatagtgcc 2280 agaagaaaaa gggaaggtag tgagaatcag attgatgact ctcctgttcg gtgcaatcgc 2340 tactcggttg ttatggagtc tcctacgaag tctaggactt taggtgtggg tcatggcaac 2400 tatggcggcg gtggtggtgg attattttca aacaaaccat ccaggatgtt ttccactacc 2460 ataaggccaa gcatggctgc tccagtggcg gcccctgctc ctgtaaatgt gtctgctact 2520 cccaagagac ggactcagcc cacgttgacg aaggagcagt acccaaggga ggataatccg 2580 ttttacaatg gaaccgatga tgctgtgatg gatacaagtc ctactaaacg ggtttag 2637 <210> 25 <211> 3345 <212> DNA <213> Artificial Sequence <220> <223> YALI0_C11165g <400> 25 atgcttggat gggcccttgg cctcgtcgtc accgaggacc cctcccagct ggaatacctc 60 aagactcctg agaccaacca tatcttttgg gcctacgcca aaatcattcc gggcctggcc 120 gccgccaaca ttgtcactaa tgctggcgcc cttgccctga agatgggtct tgatgtcccc 180 tttgtcaaca gccgggatct ctcaatcccc cccactcacg atgagctcgt ggcaggcctt 240 cagttggtgc tgcaatactc cgccgagagt ggaagagatt ggtattctgc cgtcaaccct 300 acacttccaa tttacgccat caagtgggtg gttggcaagg ccgcccagcc ttacactgcc 360 gaggacttga aggtctcccg acgaactgtc ttcaacatgt acatggccca gaccattgct 420 cgtcatgctg agaacgctct taacgcaaag attgttatca acgatcctaa tgtcactgtt 480 acagtcgtca tcagtcccgc tcctgcttcc accgctcctg ccatttcgga ggctacctct 540 atgattcctg tgacttcatc cactcaggac tatcagaccg actcttctcc tgttcctgct 600 tcatctacag ccgatgcctc ttccactggc gactctggcg cctcttccac tggcggagtc 660 gatgcctctt ccactggcgg agccgatgcc tcttccactg atggctctgg cgcctccacc 720 actggcggag ccgatgcctc caccactgat ggttctggag cctccactac tggcggagcc 780 cctgcctctt ccactggcga ctctggcgcc tcttccactg gcggagccga tgcctcttcc 840 actggcggag ccgatgcctc ttccactgat ggctctggtg cctccaccac tggcggagcc 900 gatgcctctt ccactgacgg ccctggcgcc tccaccactg gcggagccga tgcctcttcc 960 actgatggct ctggtgcctc caccactggc ggagccgatg cctcttccac tggcggagcc 1020 gatgcctctt ccactgatgg ctctggcgcc tccaccactg gcggagcccc tgcctcttcc 1080 actggcggag cccctgcctc ttccactggc ggagccgatg cctcttccac tggcggagcc 1140 cctgcctctt ccactggcgg agccgatgcc tcttccactg gcggagcccc tgcctcttcc 1200 actggcggag ccgatgcctc ttccactggc ggagcccctg cctcttccac tggcggagcc 1260 gatgcctctt ccactggcga ctctggcgcc tcttccactg gcggagccga tgcctcttcc 1320 actggcggag ccgatgcctc ttccactggc ggagccgatg cctcttccac tggcggagcc 1380 gatgcctctt ccactggcgg agccgatgcc tcttccactg atggttctgg agcctccact 1440 actggcggag cccctgcctc ttccactggc gactctggcg cctcttccac tggcggagcc 1500 gatgcctctt ccactggcgg agccgatgcc tcttccactg gcggagccga tgcctcttcc 1560 actggcgact ctggcgcctc ttccactggc ggagccgatg cctcttccac tggcgactct 1620 ggcgcctctt ccactggcgg agccgatgcc tcttccactg gcggagccga tgcctcttcc 1680 actggcggag ccgatgcctc ttccactggc ggagccgatg cctcttccac tggcggagcc 1740 gatgcctctt ccactgatgg ttctggagcc tccactactg gcggagccga tgcctcttcc 1800 actgatggtt ctggagcctc cactactggc ggcgttcctg ccatttcaac cggtggctcc 1860 ggctcttcca cagatgttga ccctgttact tccacgaaaa aggacacaga gacaaacaca 1920 gacgtctcta ctgccaccac taccggcgat aacaagggat ctcagtccgt acctgtcact 1980 gccccctctg ccacttctac tggcggttat gatggatctg atagcggaaa cggttctgac 2040 gacaatgatg atggaaatgg tgacggttct aacggtaacg gttctgacaa tgatggatct 2100 gacaacggca acgattctga cggtaacggc aatggttccg atggaaacgg caacggatct 2160 gacgacaacg gcaacggatc tgacgacaac ggcaacggat ctgacgacaa cggcaacgga 2220 tctgacggtt ccaacggtaa cggctctgat ggaaacggca acggctctga tggttccaac 2280 ggcaacggct ctgatggttc caacggcaac ggatctgacg acaacggcaa cggatctgac 2340 gacaacggca acggcaacgg atctgacgac aacggtaacg gatctgacga caacagcaac 2400 ggctctgatg gaaacggcaa cggctctgac ggtgccaacg gcaacggatc tgacggttcc 2460 aacggcaacg gatctgacgg ttccaacggc aacggatctg acggttccaa cggtaacgga 2520 tctgacggtt ccaacggtaa cggatctgac ggttccaacg gtaacggctc tgacggttcc 2580 aacggtaacg gatctgacga caacggcaac ggatctgacg gttccaacgg taacggctct 2640 gatggaaacg gcaacggctc tgatggttcc aacggcaacg gctctgatgg ttccaacggc 2700 aacggatctg atggttccaa cggcaacgga tctgacgaca acggtaacgg atctgacgac 2760 aacggcaacg gctctgatgg aaacggcaac ggctctgacg gttccaacgg caacggctct 2820 gatggaaacg gcaacggctc tgacggttcc aacggcaacg gctctgatgg ttccaacggc 2880 aacggatctg acggttccaa cggcaacggc tctgacggtt ccaacggcaa cggctctgac 2940 ggttccaacg gcaacggctc tgacggttcc aacggcaacg gctctgacgg ttccaacggc 3000 aacggctctg acggttccaa cggcaacggc tctgacggtt ccaacagcaa cggctctgac 3060 ggttccaacg gcaacggctc tgatggttcc aacggtaacg gctctgacgg aaacggtaac 3120 ggatctgatg gaaacaattc caatggatca gactctggag caagcaacgg cgttgattcc 3180 aagccccagt ccaccctcag agttgacagc actgccggac cttctgatat ccctcagtct 3240 ccccaggcca ctggatctgc tgaccaccct gcccaggcca atggagcctc taagctggtt 3300 gtcggtggct tcgctgctct tcccctgttc gctgctcttc tgtaa 3345 <210> 26 <211> 873 <212> DNA <213> Artificial Sequence <220> <223> YALI0_C13728g <400> 26 atgtatgctg aagagccagg gccagagaaa agagaggcaa aaaagaggcg gaaaaaaaaa 60 ggcaaaaaaa ggcaaaaaaa gtgtccgaaa aagtggccga aaaagtggtc gaaaaagtgg 120 ccaaaaaagt ggccgaaaaa gtggcaaaaa aagtggccga aaaagtggcc aaaaaagaaa 180 aaaaaaaaga agaaaaaaac acacctggaa tcacctcccc ctaaaagatc gcgacgcctt 240 tctggtccgg gtcacgtgat cacagctttt gtctcacgcg cttgtcatgg ctggctgttt 300 cgacgtcagt ttctaccatc ttctgaaaga caagttggtg caaagttcaa cgttgccacc 360 aaggtcacca aactaacgtc aaaagagaga ccagacggac gagcgcaaga gtggagttgg 420 agttggagtg gagagttggg ctttttgtgg tcggtttgtg gtcgttttat ggtcgtcccg 480 acatcgtcgg tcgtagtttg tgtccgtttc gccgtttgtg tccgtttcgc cgtttgtgtc 540 cgtttcgccg tttgtgtccg tttcgccgtt tgtgtcgttt cgccgctcgt gtccgtttca 600 ccgcttgtgt cgtttggcct tgtgtccaaa acacagactc caaccaccaa caccaaccac 660 caacattgcc ctcttctagc cattgacact gctctcgatt actcactgcg acagcgtctc 720 ctcgtcaccc cgtcaccccg tcaccccgtc accccgtcac ctgatcacca gcttgacccc 780 atcatcccat cacaaacacc atcaaccgct tcatctgact cccaccgact cacatttgac 840 tctgtgtttg actctgatct gactctgatc tga 873 <210> 27 <211> 2067 <212> DNA <213> Artificial Sequence <220> <223> YALI0_C14476g <400> 27 atgtccgagc tggatgagat tcgagaggac tacctgtacg ctctggagga gctgacgggg 60 gtccagaagc ccatcatcac caacctcacg gtgattgccg aggaaaacag acacgctgcc 120 aaggccatta cccgagccat tgaggagcga ataagtaagg tgagtagcgg aggaaccaca 180 atgaggagat gccgacgaca aggacgagat caaaggacac agagcgacct gaaaacaccg 240 gtgccggcca cacggtacgc cgactcatgc tctatcaaag agtgacggtg tttaataagg 300 cgtagggtgt catgttggtg atcatgtgct gcgactgtgg ttatgggtgg cgttaacatg 360 aagcttggga caggttgacg attgattttg caacgtggca catgtataca acctctgatc 420 catcgattgg cttggtccaa ctcgtgtgat ggagtttgta tctccaggct gtcgttatgt 480 cttcgagaca acgatgtgat tagtgactga cgagagatca tactgcgaat gacgacacat 540 caataatctt ttccatatgc caccgccgcc tatatctatc tctacatact aacccagtgc 600 gctccagagt acaagctgcc agcaatgtac ctgctcgact ccatctgcaa gaacgtagga 660 gcaccataca caacctgctt cggtatcaac ttgtaccgca cttttgccga cacatacacc 720 caagttcccg aatcgatccg acgaaaactg atcgagctgt acggcacctg gaaaacgttg 780 ccggccacag gcggcatgct gttcccagca gaacccatgc gaaagattgc ctcttttctg 840 gaacgaatca acgaagtcac atcacgggcc acgccaaacc ccgcaacccc caacctgggc 900 acccccaacc ccgctactcc acctctaggc actccagttc ccggccaagg aggtttcgga 960 gtgcccaatg cgggcctgac acaaccccag ctgctggaga agtgcagcca cgtgatcgac 1020 atgacttctg accgtctaca acatattcca ggcgacgtcg atgccaagga acggattcct 1080 gtcctcaaac agctacaggc cgttcttcag tcccaggcca ttcctgtgtc gtacctgtcc 1140 aatattgagg ctcaattggc ctcttctcta gcccatgagg agaagaagct tgctgaatac 1200 gaagaaaacg agcgactaaa gcagcagcag cagcagcagc agcagcaaca gcagcagacg 1260 acaccaagca acttgctagc ttccctacaa gccgctggct tgttagcagg aggtatacct 1320 ggtggaatgc ctggaatgcc tggtatgcct ggaatgcctg gaatgccggc tatgcctggt 1380 atgcctgttc cacccatgcc tatgcctggt atgccttttc ccatgtttcc taaccttggt 1440 actgttcctg caccggacat taactctctg ttggcctctg gtgcctcttc tttgctgtct 1500 tccttggagt ctaacgacgt ggaactttct acgtcttctc tgctgaagcc tcgccccaat 1560 ctggtattca acctttacgg caagatgccc aaggtgtgca acatttgtgg gcggcggttc 1620 cgagataatc aggaccatgc ccgtatgcag cacatggact ggcatttccg aatcaataag 1680 aagatgcgcc aggacgaggg acgagctcag aacagaaggt ggtacctggc cgagcatttg 1740 tgggtggcag gagagcaaaa ggaggaaaaa gaagaaaagg tgcaaaaggt ggacatggaa 1800 agtgtcaaga aacaatgggt tctggctccc agcagtgctt ctaagaagaa gcaggtttgt 1860 cccatttgca caggaggctt caacacggaa ctcagtgatg aagctgagga ctgggtgtgg 1920 acagacgcgg ttcaggttgg agataagatc ttccatgcca cctgttatgc tgagagtgga 1980 aagctggcag agagcctggt gagaaagagg agaggagaag atagagaagg aaggacaaag 2040 agggagaagg tggagctcga ctactaa 2067 <210> 28 <211> 3203 <212> DNA <213> Artificial Sequence <220> <223> YALI0_C15532g <400> 28 atgaagttca gcaaatttgt cctgtttggc ttggtgtttt tcaccacagc caattctcac 60 ccctcagagg cttacaaatc catcaagtcg attgtccatg agcacagctt gaagttggag 120 cacgtcatta gcaaacgaca ggtctctccc gataccaagt cattcattat ttccttccct 180 ggtgcttcaa ttgacgacat ctcccagcac atttcttcca tcgacaacct ggtcgcagga 240 aagtccgaac aaggcatcac tgctgacttc gatttgtctg aaagtcctga aaaccctacc 300 attattggag ttgcggctgt actcgatgag agtgtggctg aagcagtgga gcatcttccc 360 tttgctctcg ttgagcctga cggaaaaatt tacttgtggg actcactaga gcgaaattca 420 gatggtgttg tctactgtga tcagctccct ggttatgggc agcctggatt tgagcccttc 480 tatgatgatg atctgttggc ggaattgtcc gctcctactg gtgacagcaa agtccaagtg 540 gctactatta ctactgagat cattggtgta acatccgttg atggacaagc tacgaccatt 600 acatcaacca tcacctttac cagtactttg agtgagtgtg tgaggtaata atgcgttgtt 660 acgaacacct ggagcatggt cgctggttat tcatcctgtc tgttgggttc taaagtcatt 720 cactgctggt ggctagaaca agcggtgcat atttaaggtt gcgataccat aaataagctt 780 atcgagacag gatgggtgca gcacgaaaat taccctcttc accatactaa cacagaacac 840 gccggataca cccgtgagta aagcagagag cagagagctt ttaagcaaaa aaaaaaaaaa 900 atcacatttt ctgctttcgc acacacaatg gaatcccatc taacacagta ttggccgaac 960 ccaccacagc tggtgctagt gagtacctca gcacaaaccc ccgggcccac catcctacta 1020 acacagaagc cgacgcaaat gacaacaaca acgtcaatgt caacagcaac aacaacattg 1080 atgacaatga tgtcattacc ggtgaccgtg ctgaggccgt gatcgtctac actgacgacc 1140 aaggagagcc gtgtgtggcc attcgtgagt atccgttatg atgacagaga ataagtttgg 1200 cttggcgatg aagattttga gctataagtg gcgcattgct cagtcgacag agcgctctaa 1260 tattttggca acccttcata gtaattcata ctgacacagc tacacccaca tacgagccta 1320 cttgcggctg taccaagaca tactatgtga ttcgaaagct caccgaagat cacacaatga 1380 tcagcacaaa cagtaataaa ggtgagtaag aacatcatgg tttattgagt tgaaaaaaac 1440 ctaacacagt ctctcctaca ggctccaacc atggcagctc tggatcgaac ggaaacacag 1500 ctgtcgacaa cggcaacaat ggtaacagca acagcaacga caatggcaac aacatcaacg 1560 gaaacgacaa aagtggcaac gatgtcacct ctttcatcga tgacattcgt aactcaactc 1620 ctaccgcagt cgagtccact caattgtctc aatcatctgc tagctcgtca cccattctta 1680 ccaactcgag tcagtctttg gattcaattg cattttcgga ttctcaattc atcagtccaa 1740 ttgtgttatc tacaacgccc aactctggca gtacaacgcc ttctaactct ggtaccatta 1800 ccggctcgga ggtgacctct tccacctctc ccacctcttc tagtggctcc agcagttcca 1860 gtacgattct cccttcaagc tcttccgcta gcccttcaag taggccttca agtagctccg 1920 ccccagtttc ctctagctct ccagtttcct ctagctctcc cagctcttcc aaccccggtt 1980 cttccagctc tagttctccc agctctagtt ctcccagctc cagctccagt tctcccagct 2040 ccagctccag ctctagttct cccagctcca gctccagctc cagctctagt tctcccagct 2100 ccagctccag ctctagttct cccagctcca gctccagctt cagctctagt tctcccagct 2160 ccagctccag ctctagttct tccagctcca gctctagttc tcccagtgcc agctcaagct 2220 cttccagttc cacttctctc agttccagtt ctagctcaag ctcttcctct agctctagcg 2280 ctcccctccc acttgttacg caacaaggca tgccttgggg tctctctcgt atttctcacc 2340 agtcgcctga gatggctccc taccaagatc ccatggtagg agaatatatt caccaacagt 2400 ttgtggaccc caatcccaag gtggtcgtgt acgtcgttga ttcaggcgtg aatatcaatc 2460 acgataactt tgcgaccaaa cccatttggc ttgcgaatta tgctgacagt gacgactctg 2520 atgccaatgg ccatggaaca tttgtggcag gtgttgtagc tggcaccaga tcaggtgtgg 2580 accccaacct gcaagtcaag agtatcaagg tattttctgg cgagactacc gacgcgtcta 2640 ttcttatgtc aggtattact cgtgccatta atgatttcaa ggctgacacc actccaggta 2700 agaaagctgt gttgaatctg tcgcttggtg gagacgtatc tactgcgtta gattctttga 2760 ttaagcaggc tgttgctgaa ggtatgtttg ttgctattgc tgccggaaac aacatggaaa 2820 atgcttgcaa caattctccg ggacgagttt ccacctctac ccctggttct gttacggttg 2880 gttcaattga cagaagcgac aaattatctg tttacgctgg aaacaacaag ggaactgctt 2940 ggggaacgtg tattactggc tttgcccctg gttctgatat tatgtcctct atgaatactc 3000 ctaatgatgg atatggaatc ggatccggaa cctcatttgc tactcctatg gtggccggaa 3060 ttgccggcta cttgatgagt caggaaggta ccaaagatct aactcctgcg gagctcgagt 3120 caagaatcat gaacagcaat gatggtagaa ttcagggtga tctgaagaac tcacctaaca 3180 agatagccta caacggcgta tag 3203 <210> 29 <211> 1311 <212> DNA <213> Artificial Sequence <220> <223> YALI0_C16148g <400> 29 atgagactca tccctctact tctcacaggc gcctacggcc tctccattcc cattgcagag 60 gtccagaact cccccgagga tctcagctct ctcgacaagc gacagacccc tccggaggtg 120 tcccagattg tcgacgcaac taaagacctc acccaggacc ctgcctggca ggccattggc 180 caaatgctga acggaggcaa cgctgccacc atggatacgg aacaaaagtc cagcgaggct 240 gctgctgctc agaccgagat tcagagcttg ctcacaaagt actactcgtc catccccaac 300 tggcagagca ttgtgagcac tgccaccgcc ggtcttaacc agggcagtgc aaacgggccg 360 gccggaggta acggcggaaa ccctgtaaac gggggtcctc aacagtccgg caacaacctc 420 atggaccagc tcatgtccgc acaagcagcg gcatctcgac agctcgcttc tgcatcggca 480 gcctctgctg ctcgctcttc agcgcagtcc cagcctcctg cccccgccac agccgtggtt 540 gttcctgcca acaccatccc cactagagag cccgacggaa tcgcaggcac catgcccggc 600 attgtggtcc agagccagtc tcaaaactca cagtcttcga gctcccccaa cactgcccct 660 gctggtaaca ttgcaggtac acgaaccggt ctcgtggtgt tgcagggccc cacctcgaca 720 ttgaccccta gcagcacacc ccccgcaggc aacattgccg gtactcgatc tggactcatt 780 gtgttgcaga acacaaacac agcatcttct tctgcgtctt ccagaccacc ggcaccctcc 840 acggtcactg tgagccccag cagccccgaa aacattgctg gaaccagatc tggttttgtt 900 gttgttggaa acactgcctc aagcagcagc agcagcagca gcagcagcag cagcagcagc 960 cccagcggtc cctccaccgt gacgcaaacc gcagctaccc ctacagatgc cactggagct 1020 cttcttcagg gcattgctgg tcttttgggt ggaggagcgc tcggaaacct cggcaacctg 1080 gtcggaggaa gttccatcgc tactcctacc ggtggcactc ttcctactgc tcctgcttct 1140 ggttcccaga gcgaccatcc acaacccgga cctgatggag tcgtgaccat caccattacc 1200 cccggcgcca tgttccccaa ggccaccacc tttgtcagcg gcgcagttcc caagggcgcc 1260 gctcctctac tcgctgtatc tctcgctgcc ctcatccctc ttttgttgta a 1311 <210> 30 <211> 3411 <212> DNA <213> Artificial Sequence <220> <223> YALI0_C16247g <400> 30 atgcttctcc gatcacttct atttgcgtcg ttgcaaatct cagcagctct cgcccagact 60 gctctgacaa gtcccatcac ctctctggaa gtacatatcg ctcgaaagcc gaccgtcaaa 120 aaagcgttcc agtggaacac ctgggaggct gagatgggct tcgcatttga taatgtcact 180 gcttcagcca tccaaagtgg agactacttt gacttcacca tcaaaggccc tctcgcccct 240 tactccgagt cttctgaggt tcctctgacc tacgattttt acattgagac cgacaacaag 300 gagcagttgt tccacgtgac tgctactgac gacaaccact cctttcgagc caccgcaacc 360 gacttttttg actccccccc aggtgagact ttcaaccttg ctggaacttt cagtgtggac 420 tttttaattc ccgcgtctgc gccccttgtg gacagcgtct ttaccgtcgg ccctttcaaa 480 agcaccattt cttttattct gcctactcct ttgaccacgc aagccaaggg tgagaaggtg 540 aaagtccaaa tgacacgcaa gggcacttac ctcattgccg agatcttctc tttggtgtac 600 cagtctgctg gtcaacctgc ccttcccaaa gatggtaggt tcaacagact acaggccacc 660 tctggggcta catttgagcg ggaaccgagc catccctggg gctttgttga tgcgtactat 720 atcgatgtca acttgaagag atacgatggt gcagggtccc cgattgatgc agactttgct 780 cctccgggaa acagttttgt caatgcgtcg gagatgtatt ctcatctaga ctggtacaaa 840 atgcctgcta acccctccaa ggcggttgga ctgtatgtgt cagcccgtag taaaatgagc 900 gggtccgatg atagctcttg tttttactac actggtaact accgatacgt tttttcattg 960 agtcccatct gtgttgccca gggtcgagga gccgagggct tgcccaacgg agagggtttg 1020 atagcagttg acgttgttgt tccgttggct acaagttctg aggtcaccac aagtagtgcc 1080 gaggctactt ctgaggtgac caccagtgct gatatctttt ctgaggtgac ttccagtact 1140 gaagctactc ctgaggcgac ttccagtgct gaggctacta ctgaggcgac ttccagtgcc 1200 gaggctactc ctgaggtgac ttccagtgcc gatgcctttt ctaatgtgac ttccagtgcc 1260 gaggctactc ctgaggtgac cactagtgct gatccctttt ccaatatgac ttccagtgct 1320 gttccctttt ctaatgtgac ttccagtgct gatagctctt ctgtggtgac cactagtgct 1380 gatccctttt ccaatgtgac ttccagtgct gatccctttt ctaatgtgac ttccagtgct 1440 aatagctctt ctgtggtgac cactagtgct gatccctttt ccaatgtgac ttccagtgct 1500 gttccctttt ctgatgtgac ttccagtgct gatgtctctt ctgaggtgac cactagtgct 1560 ggtccctttt ccaatatgac ttccagtgct gttcccattt ctaatgtgac ttccagtgct 1620 cacagctctt ccgagatgac gaccagtgcc gaggctactc ctgaggcgac caccagtgct 1680 gatagctctt ctgtggtgac tactagtgct ggtccctttt ccaatgtgac ttccagtgct 1740 gatccctttt ctaatgtgac ttccagtgct aacagctctt ctgatgtgac ttccagtgat 1800 gatgtctctt ctgaggtgac caccagtgct gatatctctt ctgaggtgac ttccagtgcc 1860 gaggctactt ctgaggtgac cacaagtgct gatagctctt ctgtggtgac cactagtgct 1920 ggtccctttt ccaatgtgac ttccagtgct gatccctttt ctaatgtgac ttccagtgct 1980 aacagctctt ctgatgtgac ttccagcgct gatgtctctt ctgaggtgac caccagtgct 2040 gatatccctt ctgaggcgac ttccagtgcc gagactactc ctgaggtgac ttccagtgcc 2100 gaggctactt ctgaggtgac caccagtgct gatatctctt ccgaggtgac cgccagtgcc 2160 gaggctactc ctgaggcgac ttccagtgct gaggctactc ctgaagtgac ttccagtgct 2220 gaggctactc ctgatcctcc cagtagtgtc gagacttcca gtacatctga gagtgcatct 2280 gatgatgaga ctactagtgc ttcgactacc tctgaggaag ctttaaccgt gtttgtgacc 2340 gagcaagcaa taaccgtgtc tgtgatcgag caaacaacta ccgtctcttc gtctgaacca 2400 accgcatctg cgtctgaacg agaaccaacc gtgtctgcta ccaactcgtc tactgccgct 2460 cccagaccct catctagcac caaggtcttc ataaacatca atagcactga tcctgtcact 2520 gggaaggtca ccagagcgcc cgtgactagt gctttgacca cctctgagga agctgcaacc 2580 atgtctgcga ccgagcaaac aacagccgtc cctttatcta aaccaaccgc atttgcgtct 2640 gaacaagaat caactgtgtc tgccaccaac tcgtctacag cttccggacc ctcatccagc 2700 gccaaggtct tcataaacat caatagcact ggtcctgtct ctgataaggc caccagagcg 2760 cctacgttgc ttttaggata caacactact gccgcagaca gcggtaatca tgtgactgct 2820 accatcactg ttactagcac ttgtgccacc gccactgcca ccgtaacaac cggctgcatc 2880 actgttgtcg acggcgtcac ctcttccttc actgtcgtag ttgtccctag caaagctggt 2940 tctgggtctg atggttcttc caaggctgat tctactgcct caggaataca ttctggagtg 3000 attgactccg agtctgggga gaggcccaat gccgagccct ggtcagcaga gagcaaatcc 3060 gagtctcatt ttggatctac cagcacagaa aagagcgatt ctcagctcgg agacatttct 3120 gactcctctg atgttgctcc agacgtttct gatgttgctc cagactcctc tgtggttgct 3180 tccgacttct ctgaagctgc ttacgactca gcagatgttg gatattcctc tcgtgagtct 3240 attggcctgt atttgcctgg atcttcctct cgaccttcct ctggaccttt ctcgggcccc 3300 aacaactcct cctccatcag acccactccc tctactgatc aggccaacac tgcgtcttcc 3360 cttactgtcg gtgctgctgc agtcctttta ttagctgcca tggtggtcta g 3411 <210> 31 <211> 1428 <212> DNA <213> Artificial Sequence <220> <223> YALI0_D15752g <400> 31 atgtcaaact tctttgaaca ctctccggcc caatctccag ccactacaaa ttcttccgtc 60 accaacccca tccatcgaca atcctcgtct tcttcgttgc acatgatggg atatcggaat 120 cagggtactg ctggtccaac ctcggctacc ggagccacta cccccaccca tggccccacc 180 acccatgctc ccgtgcctat ggttcccgtg ggtctggatt tgggcggaac tacgtcagca 240 ccagcagcaa gcgccacggg tggactgcag agcaaccggc agtctccatc gcctctcaag 300 cgagcacaaa gccgacattt tgatatcgac acaaacaacc cggccaactc ggcgtccaaa 360 cggtcgtcct tctcttcgac cacgtcagcc tcctctgtgt ggcccggaac aagtgccact 420 gccacagatg catcgcggac tacaactccc tactacaacc tgacccaggg aatggccggc 480 ttaggcttgg gaggccctat cggcgtggct atggggtcgg gggttcctag cccccatcct 540 ggaatgggaa gtagtccttc agttgtacct ggaatggcac ccatagcccc cacccctgtt 600 gcacctaccg cggtacatcc cggtctcgct gacgacgacc ttatccccac agccatcgtc 660 atcaagaaca taccgtttgc catcaaaaag gagcaactgc tggacgtcat gacccagttg 720 cggttgccac tgccctacgc tttcaactac cactttgata acggcgtgtt ccggggcctg 780 gcattcgcca atttcaccac tgctgacgaa acggcagctg tgattggctc gcttaatgga 840 cgggagatcg gtggtcgaaa acttcgggtc gagtataaaa agatgctccc tctcgccgaa 900 cgagaacgta ttgagcggga taagcgagag cgacgcggcc agctggagga gcagcatcgt 960 ggccagggtg gccagggagg aggaaggcgg aacgtcagtg gtggtcatac tcagcagcat 1020 ggaggtgccc agcctccaca gcagcagcag cagcagcagc agcagcagca gcagcagtcc 1080 aaccttaccc aatctggttc tgtgggccaa cccgcccaca ctcttccctc acatgtcact 1140 cctcacatgt catatcccca atactctcca tcacccacac ccacaccccc tcctgccccc 1200 aagctggatc tcaacgaccc tgagactcta gagttttact cccagctcct gctgttccgg 1260 gatgacgcca aacgaacgga aattgtctac tcgcacccac tcctattgcc ccagcaacgg 1320 cagatagtca tgtcgttgtg tgaccagctg ggcctcattt tcaactccga ggcaaccggc 1380 cttgtggtgg tgacccgaca gagacagtat tacgaatacc agtattag 1428 <210> 32 <211> 3351 <212> DNA <213> Artificial Sequence <220> <223> YALI0_D17820g <400> 32 atgagcccaa aagatataaa gccagtgggt ctggggatcg ctctacccat acaagacggt 60 aagatggact ggactgatcc cagctccggc tacaacttca tccaaggtga ctttttggag 120 gtgtcaaaca ccatcgcccc cgatgccatc ggtcgcggtg cacttgacat cacagctatt 180 ccgactgaga acgatcttac tgttaagctg tttgacgagt tttgccatga cgacatgtac 240 gaatcggcga ttgattccgc ttcgcacaac tccgtcaact caacctcgaa tccaacattt 300 gggtccccct cgttggcgcc gattgattca gccccccatt cagccaacgt tacgcctcag 360 agcgtattgg ctcagcatgg actgatgggt gctccggctg ttcctgagca gcagcagtca 420 aacgtgcaca tccagcaaca ataccagacc caggtccata ctgaggatca ccagccccag 480 caagagcagg cccagactcc gtccttggct caccctcagc ggcaacccca tttccagaac 540 ctgcaaagtc agctagatca tgaacagcag acccagcatc aatctgacgc cgagcaccaa 600 acccaccacc agcaaacccc ccacccgcaa acccaccacc agcaaaccca ccaccaacag 660 cagcttcaag tcgagttgga gaatcagact acgaatgagc ctccggttgg tcactatgct 720 cagcaactgt actctgcgga ccatcaactg cagcttcagg ctcacccgtc tgggcatgct 780 gctgtccagt ctcagctgca agttcgcccc cagacttctc tgtttcccaa ccatgatcat 840 gctgagtttg ttcaatcccg gtctcgggct cagcttcaag attctcagct ggttcatctt 900 cagcgaccag gccagactca gtttcatcat catcccactg ctcagtctca tattcaaact 960 cagaatcact tgaaccacgg cgatcctcct cagacaatgt ctggtcagat tcattttgat 1020 cgccagactc agttgaaact ggagttcgag ccacatccac atcctcctgt tcagtctcat 1080 tctggagttg gcatgcaatc tcatgctgag acttttcaaa atggtgctgc tcaacctcaa 1140 ccgagtgctc tgcagactgg ctctgcgcag ccttctcagg ctcagaacca acatccttct 1200 gccgtatcgg cgcagtcgac acccgaaact gtcccctcgc cctgtctgaa ctctccgccc 1260 gtgcatgcaa atgatacctc tcctggaacc gtttctgccg gaaagcagtc taccactcct 1320 cagttccctg ctcagtatag ccagagtctt gatcagagta gtagtgagtc tcagctgcaa 1380 cggcagcaac tggttgtcca gaattcgtct ctcatttaca accagcagct tctgaactca 1440 ccattccaag ttccccaaca agcacaattc caaccccagc tcggagaacc gtcgcaggag 1500 ctgaccatgg ctcagtttcc tgctcaacaa gtgatgtctc cggtaactcg cacgcttcag 1560 tttcacgtgg gtgctgctcc ttctaacgcg actctgtcta accaaccatc acctaaccag 1620 ttccagatga ttgtcgctcc ggcagctccc atcggctttt cctctcgtcc ttggagaaca 1680 gacccgtttc aacgatggta ttctgcacgg atccagatgc cgttggaacc tgagacggca 1740 gaggatgtga acatctgcaa ccggcttgag cgagcggttg ccaaggtcaa gatctgcgag 1800 gacctgcagt actcgaccgt cagggcgtgg gatatcaacc aacctctgac gctcggacag 1860 tacgacgcca tgaacaagtg tgaaggtgaa atcgtcgagt tgtcacacca tcgacttgac 1920 gagcgagagc agcgagagaa ggatgatgag aagctggctc tggagtgttc caggaacgag 1980 ttactggctc tgcatcttcg aaaccaactg atgcagttca aactcatgtt tcatggccac 2040 cctcatttcc acgcgctccg agtgtgtaac gaaacctgtc ttacgcgata ccctgcatat 2100 ccccctcctg ccaatgctgt ggttggagta gctggaagta atgcttcgac cgacaatatg 2160 aagctgtcac cgccaacact cctagaaccg ctctcaccac aggagctgcg gcggctagaa 2220 cagctcgagg aagaaaacga aaacgactac tccaactttc cttgggccca tagattggtg 2280 agcttgacac agcttgagcg atggaagcaa cgaacgcaca ttgtcggtga gctcttctac 2340 tcgtcccgac caatacacgc gaggcagata cgcaacattg tcaggtttct ggaagatgac 2400 tttatgcaca tcaaggtgac ctctcttcgc gcccgcgagg tactccggga gcacttcaag 2460 aacccctacg tcactctgtt tgaccacctc cagcgtcaga gggtacctga acagaggaat 2520 ccccacatct accagtacgt tgagtcgatc atggacaatg tctacctgtc ggcctactac 2580 cgagtggagg ctaagaaggg cacacggaag ctggatggcc caggaggcga atacattcca 2640 gacgagaagg atattggaga gcggtactgt gggatatgtg acaagtggtt caagacgcga 2700 aaccacaatt gggcgtccca tatgacatct actcacggtg tctgctctgc aaccaagtcc 2760 atctaccctt tccctctagc tgtcattctc ggcatacctt ctacgaccca gaaggaggat 2820 gggcttgtgg tccaccagaa catcatcttc gagactaact ccgacggcga tcttcagatc 2880 cagaacgaat cccagccacg tcaccatctg ctgcccgaca aggacatcaa gctcagtggt 2940 ctgtgtccca tttgctgcca ctatgttccg ctctattgca agcgaggtgc cactgtctgg 3000 accacctggt tccgacacca ggacaagcat atccgggagt tcaaccagga acgaaacgga 3060 ggtgtggctg tcaaccacaa caatccacga aacaacaaga cacgaaaaga gcagatttgc 3120 aaggagcgga tgcaggcctg gagacagcaa caggaggaac ggatgagcaa gcgactcaaa 3180 aacgacgata tggcgttcct ggaggagggt gtggttgtcg ataatgttca aaatggcagc 3240 tggctcaatg aggagtttgc catgacccag gccgaggtca cgttgacgta ccagatgggg 3300 tatcaggagt ctgagcccgg tattgggagt ggcattcagg acttttttta a 3351 <210> 33 <211> 2907 <212> DNA <213> Artificial Sequence <220> <223> YALI0_D18381g <400> 33 atgattgcaa aaatacccct acttccgttc gtggtaggag ctctggctgt gtccaactcc 60 accgactcca ctctcaccga ctccactctc accaactcaa ctctcaccaa ctcacctctc 120 accaactcaa ctctcaccaa ctcaactctc accaacttca ccaactctac ccatgttccc 180 ttcacttctc ccgatttcta tcctactccc gaaattggca ctctggacgc ggacaagcgg 240 tggagagctg ctctgaacga gtccctggag attctgtctc agctgactct tgtcgaaaag 300 gtcaacatca ccacaggtct tggttgggga ggaggcacct gtgtcggcaa cactggcgga 360 gttcccagac tcggtcttaa gggtctctgt ctccaggacg gacctctggg aattgctcag 420 accgactacg tgactgtttt cccctgcgga attgccatgg cggccacctt tgacagaaac 480 ctggtccacc agagaggcac cgccattgga caggaggcca aggccaaggg agtcgatgtc 540 catctgggac ccgtcgtggg acctctggga cgacacgcaa cgggcggccg aaactgggag 600 ggcttttctc cggaccccta cctggccgga aagctcgtgt ccgaagctat ccgaggtatc 660 cagtccgaga acgtcatggc caccgtcaaa cacttcattg gcaatgagca ggagcactac 720 cggctctact ccgagtgggc tcgtttcgga ttcgacaacc tcaccacttc ggtatcctcc 780 aacattgacg atcgaaccat gcacgaggcc tacctgtggc cctttgccga cgccgtcaag 840 gccggagtgg cttcagtcat gtgttcgtac cagcagatca acggctcgtc gggctgccaa 900 aacagcgcca cgctcaacgg caagctcaag tccgagctcg ggttccaggg ctttgtggtg 960 tcggactggc aggcccagct cagcggagtg tccaatgctc tagccggact ggacatgagc 1020 atgcctggta acgacgtgga cggaaacatc ttctggggac cggacttgac caaaatggtg 1080 gctaacggca ctcttcccga gtccagactc gacgatatgg tgctgcgaat tctcacagcc 1140 accatctaca caggcatcga cgaaagagag cccaccttct ccgccttcac tactgaaacc 1200 tttggcaacc ccaaccccgt gcttatgttc aacatcaact acacctccac cttggtcaac 1260 ctccatctgg acacccgaac cgccttttct tcccgtgtgg cgctggaggg agccgaggct 1320 gcagcagtcc ttctcaagaa cgacggaatt ctgcctctgc agggccccga gaatgtcgga 1380 gtgtttggag tcggctcgca aatcgggcca aagggagcct actgcgggtt ctccatgcaa 1440 tgctcggatg gagctctcat cgagggatgg ggaagtggaa ctgccaaccc aacagagtac 1500 acttctccgt acgaagcact ccgacaacga gccaaccaag ctggaggcca cgtgattgga 1560 accaccgagt cctggaacat gacgctgcct ctgatcatgg ctgacaactc ggacgttaac 1620 atcatctatg tgctgtccaa ctccggagag ggaggttcga ccgttctgga caacatggga 1680 gacagaaaca acgtgtctct gtggcacaat ggcgacgaac tgatcaccac tcttgccaac 1740 agctccagaa acaacattgt cgtcgttacc accgttggtc aggttgattt ggagccttgg 1800 atctcgcatc ccaacgtatc ggccgtggtt ctcactggtc ctgctggagc ctatggaggt 1860 aaagccatgg ccgaggttct ctatggagac gtcaacccat cgggaaagct gccttatacc 1920 attgccaagg acccccaaga ctacattccg gtggttgtgg acattcccga agatggagct 1980 cctcaggcct actttgatga gggtgtctac atggactaca agatgtttga caagctcaac 2040 aagacagttc ggttcgagtt tggttacggg ctgtcttatt cggatttcga gtttggagac 2100 ctggatgtgg ttgtagacga gttcatttcc gagcttcttc cttctcctcc tcggcctatc 2160 attgttgaca agcccagcag caccagctcc aactcgtccc acggcgatct caaggctccc 2220 aagggtttca agcccattcg aggagttgtc tatccgtgga ttgacgttaa ttccctgact 2280 gccggcgaag ctctttcggg agtgagtcct gctcttgcca ctttgctggg tctaggtaaa 2340 cctgatacct gtgctgttcc cgccaagcac gagactggcg atgatgacaa ggactccaag 2400 tctgacgata agaccaagag agacaccgag tcggatgctg ccgatgatga aattgaaaca 2460 gtcagcacct ccaacgaaac cgccgttgaa accaccattg gaatgaagaa cacctccgag 2520 cccttccact ttgccaacgg aacagggacg tcaacaaacg ccgccggagg agttggagga 2580 aacccatctc tctggaaaac cgtcgccacc gtgtctcaca ccatccgaaa cctcggtccc 2640 tatccaggag ccgcagtcac ccagatgtac attgccttcc ctcaggacga cattgattcg 2700 cctctcattc aacttcgggg atttgacaag actcgggttc tggacgtcgg agaactcgaa 2760 actaattcct acgacatctt gtggagagac ctggctgttt gggacgtggt catccagagc 2820 tggagagtcc agagaggaga gtacaagatc tacgtcggca actcctccag agactttgtt 2880 ctcatcgagt cctttactct ccagtag 2907 <210> 34 <211> 2421 <212> DNA <213> Artificial Sequence <220> <223> YALI0_D19822g <400> 34 atggaaggcg tggtggagaa cacaaacaag atcattacgt tgattggcga cggctggctg 60 gtggaaaagt cggcatttgg ggcactcaag gtggtggatg accgtctggc cattctggag 120 aagaatcaaa aggcctatga ggaggctttg gagacagcca agttcgagaa ggagggccta 180 cctgtgtttg agattcgaga ggagctggac gacaagggaa atattgtaag ctcatctgtg 240 gaggagtttg ataccggtaa agctgctaat cttgagaggg ttatcaactc catgggagac 300 cggaagaaca tcagccatga cagcgacaag atggatgaag acgacgatga gatggacaca 360 aaggaggatg atctggacga cagcttcgac ggtgcatctc tgcctatgat ggagattaga 420 gaagagctag acgacgacgg gaacatcatc gagagtaaag tggaggaggc tggaaatgag 480 gacacaaatg agattgagaa gcatctgaag ctgctcaacg agctagccaa aaagaagttg 540 gatgcaaaga gtactacaga gcctgctgtc aaccccgaag ctgctaaatc taccaaacca 600 gaaactgcta aacccatcta tggtcctcct ccgcccccca actatggtcg aaaggttgga 660 ggcggcattt tcaagcccca agttactcct ggagtgctgc gacagcagag tcccaagatt 720 gaggaactta acgacgaaga agaacaagtg gaggacggca aggataacaa aaagactaca 780 gagcccgaaa aggagaagga caatagtcct acaatcaccg atgtcacgga agatgactcc 840 agtgactctc tttccagctt gcagtcgtct aactcgtcta tcacttctaa ccctgctgtt 900 gcgcccgagt cgcttcttga actggagctg ctagctgacg atgctatgga cgaagaggag 960 gacgatctgg aagccgatga cgacgactgg gactttgaag acgacgagtt agacgacgtg 1020 gaggccgacg aagacgacca tgggcgtacc cggggttcca tgttcccagg aatgtctcag 1080 gatcaactga gaagcattat ggagggcaga aaaaccgctg ctgctgagtc gagacctcct 1140 gctgcttcta tcgtgtctga tatcaccgaa tcaccccagc ccgtttcttc accagttcct 1200 actactgctg ctgttccaaa gagcagtttg aagtctgcat cttccaaacc caagtctgta 1260 tcgttttcca acaatctaca tatcaaacga attcccaaca aggagaccat caagaaccag 1320 gaacgtcagg agatgaagaa gcagcagcaa cagagcagat tcaagcagga gctggcccgg 1380 aaggcccctg aacctgaaga tgacgacgat gaagatcata ttaccgtgcc tactgtctcc 1440 gatattgctg aacgagctgc cccagaagac cctgtgactg cccctgctcc tcccaagaag 1500 atgtcgcgat tcatgatggc tcgaatgggt aaagatgagg ctcttgcgcc caaacagtcc 1560 cgtgataccc aatcacatga tcagacaaac cccaaggatg gacgcgtcat aagaggcatc 1620 cccagtgttg gtgctacttt cccacctact cttgacaaag atattgctga ctacacagag 1680 tcaatgaagc tgaccccccg accggcttct cagggacacg gccatgcaca tgggcatgtg 1740 agatcacgag caatttctgg gaatgtttct gagccagctc cagccgagga gaacgcccct 1800 tccaaggttt tcatcaaggc tccagtgtct ggacttgctc aaggcaagac taacgacgag 1860 cctcgacctc tgaccgatag cgacaaggag cacgatgacg ccatcatgaa ggaggtcatg 1920 tcgtatcttg ctgaaaatga ggatgaggag gctttcgagc agctgcatca ggacatggac 1980 gagtctaagc tggctgagga gctgtataga cagagggccc agaatcacaa tgctgctgtt 2040 cagcgagggc tcgaaagtat ggccgatgtt gacgtggatg atggagacga cgatgagatg 2100 gatttggacg atgatatggc tgatgctggc gacagagttg atgcggagaa cgacgagaat 2160 gcacaggtca tgaatgatat cgttgagaac gatattgatg atccttcgaa agacattatt 2220 cagggagatc tcgatatgga tcctgccgtg catcgacggg aggttgccga gcactactac 2280 cggctgcgaa acaacatggt tgccaagaat ggcgggttta tgaaatccga cggtgaaaag 2340 gccgccgagg agtgcgatga gaatgccgct cccatcaaaa tgtctcgatt caaggctgcc 2400 agaatgaacc gagggcagta a 2421 <210> 35 <211> 1493 <212> DNA <213> Artificial Sequence <220> <223> YALI0_D20064g <400> 35 atgtcacggt tcgatatcga cgaaacaaaa tccaaaacag acgactccaa cggctcccag 60 cctctgtggg gggggactgg agcacatgga gaaagcaggt gaacttcgcc tttgtcgctc 120 tcttcactct cacatctttt gcaacaatgt gtcaagcatc cgttgtctgg ggaccccaga 180 caagggatct gggctggcag tttgaacagc tcaacagagg ctacgctttt agcatagctg 240 gccagggttt ggggtgtctt ctgatgatcc ccgtggccat caaattcgga agacggccgg 300 tctatctctt tggtgtggct ctgtcattgt gcacttcgct gtggcaggcg caaatgacgt 360 ctctccatga gctgtacaca gtttcgtttt tagaaggcat ggctgccagt ttgaccgagg 420 cgtgtgtcca aatgacgatt gcagacctat tcccggtcca gagcagaggc acctacaacg 480 gcatgtacat cattgcagtt tcggtgggca actttctcat tctggtgcct gctggagcca 540 tagtcaacca gtggggctgg agagctgcgt actgggcgat tggcctgttt cagatggtcg 600 agttggttgc atctttgttt ctgtttgaag aaactaaacc tggccatgtg tctggtcaaa 660 agttgagcat atatcctcgg tcatcttcaa agagtgacga agaggagaga gaaacattgg 720 ttaacgacat ggaaagacac aactcgtatg ataccgagag aagcgcgtcc agaggatgca 780 tcaagtgcac cgactcgttt ccacatatcc cgttggtcac agttactcct ggctggtcga 840 gtttcttcaa gtctttctac actccgtttg tcctactggc ttcgcccatt gtcgcgtttg 900 ccgcaatcac ctatggattt ttgctgctgg ctctgtcaat gtcgtcggta gtgacttctc 960 tcaagtttgc agaagccccc tatagcatga gtccctggtc cattggtctg gtgttcattt 1020 ctccgtttgt gggcatgacc atgggctcct ttttcggcgg gtacttgtct gactgggaca 1080 ttgtgcgaag aacaagactc aacagtggcg tctacagtcc acagatgcga ctgaaactca 1140 tctacccagg tctattggcc gtcacggcag gtctgctaac ttttggcatc tcggtgtcaa 1200 gaggagttca ttgggtggtg ccaactcttg cgtttggagc tgtttctttc ggatttggaa 1260 gcgtgggatc catagtattg acctatctga tcgactacaa ggagtctctg gctgctcagt 1320 ctctggttgc tgttgtggtg gtccgaaact ccatctgtat ggtggagtcc ttctccttgt 1380 ctccgtgggt ggattcggtt ggggttgaca atgtgttcat cacagcgggc tgtttctcgc 1440 tgatccccat ctttttggca ttggcgattg agctgtttca tcgcaagtct tag 1493 <210> 36 <211> 619 <212> DNA <213> Artificial Sequence <220> <223> YALI0_D20526g <400> 36 atgtctcagg gtgagtattc agatggagag tgaagcgtgg gttggatacc tagaaacctc 60 tgagagcctg agagcctgag agcctgagag cctgagagcc tgagagcctg agagtcatca 120 acagactagc gtccttggct ctgacaggtc cattccatcg tcagacgggc gattcggaga 180 cagccacaga ctcctgtctg atgtccgctt tagaccatgt actaacctag ccggccgaaa 240 agatttcact gacaagctct ccgagggcgt gacccccgac tcccagaagt ccactggcga 300 gaagctctcc gagaaggcca ccgacgccta cgacaaggtt gccaacgccg ttgatccacc 360 ggagcccgag tcccagaagt ccaccaccca gaaggtcggc gacaagttcg agtccgacac 420 ctccaaggcc aagtccgaca tctccgacga gaagaacaag ctcgagaagg agggcgagtc 480 ttacattgat caggccaagg acttcatcaa ctccggcaag ccccaggagt acgttgacca 540 ggccaaggag tccatcaaca acttccttgg tggaaactcc ggctccaccg gctccaccgg 600 caccggtgct accaaataa 619 <210> 37 <211> 2883 <212> DNA <213> Artificial Sequence <220> <223> YALI0_D20790g <400> 37 atgagtgata aagaacgcga acgtgaaagg agtcaatcac gtgaaagtag ccgaacacgt 60 gaaaggagcc gaacacgtga aaggagccga acacgtgact cgaagcgtga ccattccgat 120 tctcgatccc tccggagacg cagttatcgt ggcgacagat cgagaagccc gtcacgatct 180 cggtccaggt catattctcc atctcggtcg ccccgaagga agcggcgccg acacagaacc 240 agtcgcagaa gccggaggtc acgaagggaa ggtgatggac gacgcaaaga tggccctaga 300 ggccatgatg acagaagccc cttggcccct agggaggtgg aggatgtgac tgtcaagagg 360 gaggatagca cgaacgacaa ggaaagtact cacgacaagg aaagtacaaa cgacaaggaa 420 cgcaatgtct cgatggagga caggaaggat acttctgagg atgttgtgga ggatgtggag 480 gagggggatc cttctgaaga ggcactggaa aaaaaagtaa agaacccaga agatgacttg 540 gaggcagcgg cagaagagct caagaaactc atggagctaa agtctggagg ccgatacgtt 600 cctcctgcta aaatcagagc gcttcagaag ctgctggtcc aggataagac gtccaaggag 660 ttccagaaga tccagtttga caatctcaaa aaggcaatca actcgctggt gaacaaggtc 720 agtgctcaga acattcgaga tattgcgggg gagattttca ctcataatct catccgagga 780 cgtgggctgt tctgtagaag tgtcatgacc gcccagagtc ttgctctgcc ctatacgcct 840 gtctacgcct gtctgacggc cattgtcaac tccaagctac ctcaggttgg cgagctgctt 900 gtgcgacgac tcattctgca atttcgacgg ggctacaagc gtaatcagaa ggacgtgtgt 960 ttgtcaagtg tgacgtttct ggcgcatttg tgcaattacc atgttgctca tgaggttcta 1020 gtgcttcagt tgctgcattt gttgcttgag acccccacag atcactctgt cgaggttgct 1080 gtagctttca tcaaggagtc cggtgccttt ttggccgaag tttcgcctgc tgccaacaac 1140 ggagtatttg agcgtcttcg agctgtgttg catgatggcg agctggaaaa acgaacgcag 1200 tacatgatcg agacgctgtt ccagatcaga aaagacggct acgaaaacta ccctgtggtt 1260 caagaagaac tggacctggt agacgaggag gactatgtga cacatatgac gggtttggac 1320 gacaagttta cagatgataa gctgctcaac tactttgtta tggaccctga ctacgaggcc 1380 aacgaggaga agtacgattt gctgaaaaag gagatcctgg gagactctga cgacgaagag 1440 gaagatgata gtgaagccga ggaagaggca gatgatgaag aggaggagga gggggatgaa 1500 gaggaggagg ctcaagcctc tacctcggct gtgcgagacc tcacgggtac agagcttgct 1560 acgttgcgaa agaaaattta tctgacggtc atgtcgacca tgtcgatcga cgaaatcgtt 1620 cacaagcttg tcaagctgtc tcgaacggtt attgagatcc ctgagggtct ccccgaagac 1680 caggctctga ttctgcgtct caagcggacc caggaggtga cgaacatgct tgtggagtgt 1740 tgtgctcaag agaagattta taacaaaatc tacggaggta caggagagcg gcttctgaga 1800 ctgtcgcggg aatggagaac caactttgag aacacctttg ggttctttta ctcggttatc 1860 cacagatacg agcccaatca gatcagaaac attgccacgt tctttgggta cctgttggca 1920 tcagattctc tgtcgtggaa ggttttggag gctgtttctc tgactgaaga ggactcgaac 1980 ccctcgaacc gtatctttct caagattatg ttcaccgaga tgcgtcagga gctgggaatg 2040 gatctgttga aggagcgact ttccaagccg tttgtgcagc agtacattgc cggtatgttc 2100 cccaagacga atgcttctca tgttcggttt gccatcaact atttcactgc cattaatctt 2160 ggtcctttga cagatggtat gcgggagatt cttcctggtc tggtggagga ggaggagagg 2220 ctgaagaagg aggaggaaga gcagaagcga gagtacgaca gttacgattc atacgacagt 2280 tatgactcat acgactccta cgactcctac gactcctacg actcctacga ctcctacgat 2340 agttacgact cctacgatag ttatgactcc tatgactcct atgactccta tgactcagtg 2400 tctgaggatg atcgggggcg acgacgacga cgaggtgtag gaagtagaag gcggtcgcta 2460 actcggtctc ctggttcgcg gtcacggcca cgatcccggc cacgatcccg gtcacggtca 2520 agatctctat ctcgggatag aaccggttct ccgcctcgtg gtcgacggcg gtctcgatct 2580 tattcccgtt cgccttcgcg gtcatactct cgttcgagat cttattcccg atcgccatct 2640 ccgagaccta aatctaaagg ggctaaaggc cggagcagtt accgggccgc cagttattcg 2700 cggtcgagat ctccggtcag acgagttggt tattctagaa gcaggtctcc aagcgtgtct 2760 ggaggttcct ctcgaagtcc ttctagaagt ccttgtagaa gccccaacaa gggccgtgct 2820 cctactccgg agaaaggtct ttcgccgcct cgaaagcgag tgcgcgccag tgatttgttc 2880 taa 2883 <210> 38 <211> 633 <212> DNA <213> Artificial Sequence <220> <223> YALI0_D24563g <400> 38 atgactgaac aggacgacaa ggcgctgaag ctggccaaga aggaggccaa ggaggccaag 60 gaggccaagg aggctaagaa ggaggctaag aaggagaaga aggagaagaa ggagaagaag 120 gagaaggaaa agtccaagaa gcgatcggct gaagatgatg atgtcaagga gatcaagaag 180 gccaagaagg acaagaagga aaagaaggaa aagaaggaaa agaaggaaaa gaaggaaaag 240 aaggaaaaga aggaaaagaa ggaaaagaag gaaaagaagg aaaagaagga aaagaaggaa 300 aagaaggaaa agaaggaaaa gaaggaaaag aaggaaaaga aggaaaagaa ggaaaagaag 360 gacactgaaa aggaaaacac tccatctctc gcctcccccg cattcctcgc ctcccccgca 420 gcaacctcaa ccccaaaaac catagccgtg gatggcctgg actccgccca acaaaacaag 480 ttcctgcgac tgatgggagc ctccaaaaac aaggtgaccg tcaccaccaa ctccacctca 540 gaccactccc agagagaaaa agatctgatg aaacaattca accagggcgt gaatctcaag 600 acgggtggaa agaaagccgg tatcggaaaa tag 633 <210> 39 <211> 270 <212> DNA <213> Artificial Sequence <220> <223> YALI0_D24849g <400> 39 atgatacatc agtcagctga caccagtgaa cacagccaac tcagcaactc agcaactcag 60 tcgttcagca actcagcaac tcagtcatcg accaactcac caactcacca actcaccaac 120 tcactgactc accaactcac caactcactg actcaccaac tcaccgactc accaactcag 180 tcttccgcta atcaccaatc acatgactgt ggcttactac tcatttccct accacttgac 240 cacttttttt tcgttttcac acggtgctaa 270 <210> 40 <211> 1533 <212> DNA <213> Artificial Sequence <220> <223> YALI0_D25014g <400> 40 atgcacctgt ccacctccgt tctcggtgcc ctgttggctc tgggccacgc cctgcctttt 60 gatacctcca gtgtcgctgc caccgacgcc tttgctgcca ccaacgccac ggccctcgcg 120 gccaacggca ctctcaccaa tggcaccttc agcaaccaca ctgccccctc cgcaacctcc 180 cagcttgcct cactcactca gctgaccagt gatacccccg gcgaagccac cagtgccgcc 240 gtttctgccg ccgcagcaga ctttgccgca gccgccgtga ccgtgaccgt gaccgccccc 300 gccaacaccg tcaccgtcac cgagactcct gctccccaga ccgtgaccgt gtccgtctcc 360 tactgtccca cccccgcgcc tactggtgag gctgccgccg gtgcttccga caacaccgcc 420 gccgccgcag ctggaacccc ctccaacaag caggccggcc agaacttcaa cgccaacgag 480 gacgccgcta gcgccaactc tggctcggag cccgctggcg ccaactctgg ctcggagccc 540 gccggcgcct ccgattcctc cgacgaagaa aacacaactg tcttcctcac ccagaccaag 600 ttcctgacca agaccctctc ccagtacgga gtcaccacca ccgttcccac cgccaccgtc 660 acctctgcta ttgtcaccaa ggccaatgag gagaacgccg ccgccgcctc ccccattgtg 720 actgacatca ccgagaccct gaccgaggtc gagaccgttg cctctgagga acctcaggga 780 acgtctactc gaactcatta catcaccaag accaacacca ttacccgggt gagatcgtcc 840 actgcccagc ccgagacttc caccgaagct cctgaggctg ctgccggagc ctcctcttcc 900 atcgagacct cctccgaggc ctcctccgag gcctctgtgg aggccaccgc ttcggtcgag 960 ccttccaaca cgtcatccga ggttgccgcc gaggccccca cctccgctcc ccacaacgca 1020 accttcaatg ctactcaggc tcccgttgag acctcctcca cccccgtgct gtcgaccact 1080 tctcttgtgg cccccaccaa cgccaccgaa gccggagccg ctgctcccct cacctcttct 1140 tccgagcccg agggaacctc caccacctac gtttcctcca ccgtggtcgc tactctggcg 1200 cccaagacct cgtctatccc cgagggcgct gccggagccg agtcttccgg agtgtcttct 1260 gaggcctctt ctgagctccc ctcttcagtt gcttcttcct cggctcctgc ttccaccacc 1320 gctcccgaag gcgctcctga aggagctgct gccgtctctt ctaccgcgga ggcctctgct 1380 gaggcttctt ccccggctcc ttcctctgcc cctgtctctg ctgaggccgg agagtctgcc 1440 gttccccttt ccgaatcggc tcccatcccc tcccctgccc ccacagttgc ccccaccggc 1500 tttgccgcta acggcacctt cacctctgcc taa 1533 <210> 41 <211> 1638 <212> DNA <213> Artificial Sequence <220> <223> YALI0_D25058g <400> 41 atgtctacga ctgaattcga cggcatctat ctcaatcaga gcaaggcaca cggccgtctg 60 cggatggttg agacgggcct gggctggaag gcagtgcaga agacgtcaat gggcggatcg 120 aaggagacca agaaggacga gccgtttcta cttggaaagg aggagctgct ggcggcgttt 180 tggagtcggg gctctcgggg attcgagatg aagatccaga cgaaaaaccg cggggccgcc 240 aactttgacg ggtttgaaca ggacaacctg gaggagctga agaacgtgat gaagcgcaac 300 tatggcattt cagtggagca gcgcgagcac agcgtcaagg gttggaactg gggcaagacg 360 gactttgagc gatccgagct ggtcttttcc gtcgccaaca agcccgcatt cgagatcccc 420 tacgccgaag tggccaactc caacctggtg ggcaaaaatg aggttgctct ggagttccag 480 cagcccgccg atggacgggc aggcgacgag ctcgtcgaaa tgcgattcta cgtgcccgga 540 gtaacttctg tggaaggtga tgagaacccc aagaagaagc agaagacgga gaagaaggag 600 ggagaagaag gcaaggaagg agacgacgac gccgacgccg acgacgaatc agaagaggag 660 gtccagtcca ccgcccagat cttctacgac accctcaagg aaaaggccga cattggagcc 720 gtggcaggta cggctgtggt gtctttgtcg gagatctacc tggtcatccc ccgaggacga 780 tacgatatcg acatgtacgc caacttcatg cgacttcgag gaaaaacata cgattacatg 840 gtgcagtaca agcacgtgca gcggctcatt gtgctgccca agcccgacga tctgcacaac 900 attctcgtgg tgcagctgga tcctcctctc agacagggcc agactcggta cccgtttctg 960 gtgatgcaat tcctgcgtga ggccgaaatc aaagtcgagc tcaatgtcga cgacgccgag 1020 tttgccgaaa agtacgccga caagggtctc aagcagtcgt acgacgagtc tgctcaccag 1080 gtggttggat ctattttccg cggtctcact ggccgaaaac tgaccgtgcc aggctcgttc 1140 aagaccgtgc atggccatgc tggtgtctcc tgttcgctca aggcgtctga gggccatcta 1200 taccccctgg aacgaaattt cctgtttctt tccaagcccg ttttcattcc ctttgccgag 1260 attcaagata tcactctgtc tcgagtgggt tcttcggtaa ccacctctcg aacgtttgac 1320 atgactctca agctgcgaaa cgcccagggt gagtaccagt tctccaacat ctccaaggaa 1380 gagcaggagg gtctcgaggc gttcatcaag tccaagggca ttcgtcttaa gaatgatctt 1440 gctgaggaga aggcgctgct ggctgccact ctggctgagg tggacgatga ctctgatgat 1500 ggaggagagt ttcgaggctc ggctgatgag gatgacgaga gtcctgatga ggactttcat 1560 gccgagagtg atagtgaggt ggcggaggag tttgactcga atgccgagag cagttcggga 1620 gaggaggatg acgagtga 1638 <210> 42 <211> 1971 <212> DNA <213> Artificial Sequence <220> <223> YALI0_D26257g <400> 42 atgaagttcc caatcgccac cctggcagct gttgcagctg tggtttccgc tcagtacaac 60 ctgtacaatg cgaccaatgg cacgactact atttcttctt cagcttctcc ttcgaccaac 120 tactctgcct ctttgaccac ctcttcctct tggtctgatg atggtaccca taccactctg 180 cccatttggt ccgaactcat gtcggacatt tcttctctgg agtcaatgtg gaacaacagg 240 accacttcgg ccgagtctgt tggtcccacc acctattacg tcgacagctc cactccggtg 300 gaatcttcta cgttctcctc agcttcttct gcttggaaca cttccactgc gatcttgacc 360 gcttccaaca cctcgaccat tgcgtatcct actaatgttg aacgagctgt tccagttccc 420 accgggtttg aggacggcga cgagtatgac tatgatgaag acacagagga cgaggacgat 480 gacgaagatg aggaatactg gtactccacc tctgctctgt ggaacacctc taccgctcag 540 gctacaccta ctcccatttg gaacacgtct acctcctcca ccactctctg gaatacctcc 600 actgtcgtgt ggactgagac tgctacctcg tctacccctg cctggaactc ttcaacctgg 660 tggaccgagt ctgagacgca gacttccacc gcttggaaca catccatttg gtggacttct 720 tcttcttctt cttctgcttc ccccactact ctctggaaca cttcttcttc agccactcct 780 acgcccattg tgaacacctc ttcctgggtc tggagctccg tcgagtcctc agctagcccc 840 acctcgatct ggaactcttc cactactgct cccatctatg agcgagactt tgacactacc 900 tcgtcttctt ccatgaactg gacctcctgg taccaccaca ctgtctcgac ttcttcctgg 960 tattctggag accccgagtc cacctccaag gccgtgacca cctctcacgc tacagccaca 1020 tcttctgtct ggtccaactc ttccaccgag acgaagtact ctagctccac ctggtacaac 1080 tcttctactg ctgcctaccc cacgagctct tctatttccg agaacagcga gtactggaat 1140 cccgttcccg tcaactccac catcatttcc cctccatacc agcgacaccg acagaacacg 1200 tctacctggt ctgagtctgc acccacttct tcgtacgtgt ccaacacaag gaactcctcc 1260 accgcctatc ctactacctg gtacaactca tccaccctct ggtcctccat ccccatcagt 1320 gtccccactg gtatcttcaa catcactact cctctgacca cagctgctcc cactactccc 1380 tcgtacaata cctctctggg tttcgaggag cgagactatg agactgacga cagctactgg 1440 gagtccgaat atgaggaata cgaggactgg gaggacgcgt cgactgagta ctcctggacc 1500 accagcagca gcaatttctc caccgcctgg ccttcttctt catcctcttc cacctggtgg 1560 acttcttctt cggccttcaa ctcttccact cctgtgactt cttacatctg gcccacctct 1620 ttggtcaaca cctctaccat ctggtcttcc agctatgtgg ctaacaccac caccaccgtg 1680 gccttctcca ttcctaccac caactcttct cgatctaccc gatctgcccg atccaggtac 1740 tcttcctctt ccttctctac tccctcgacc acttctactg atatccggac ctcttgggcc 1800 cccgaggatc tcatcgagca gcctgagccc actaccagct cctcccactt tgacgatgct 1860 ccgttcccta ccgccccact gcctcccctt gagtctggag acgaagcacc tctggaaatg 1920 cagtaccctg actcctttat cgatctcccc tgggttgatt acgagagtta a 1971 <210> 43 <211> 1838 <212> DNA <213> Artificial Sequence <220> <223> YALI0_D26510g <400> 43 atgtcgaagt ccatcgtccg ttccatcaag aacgtcacga acggatacac ctcggcccag 60 gtgaaggtcc gaaatggtga gtatacaatt ctcccctctc tactattgca caatcccctt 120 ctcatcacca ccgttttttt ttctcgctct actaacatct agccacatcc aacgacgctt 180 ggggtccgtc cacgtttgac cttgaggaaa tcgccagact cacccactcc aaccaggagc 240 ttttcgaggt gatggacatg atcgatcgac gactcaacga caagggcaaa aactggcgcc 300 atgtcatcaa ggccctcaat ctgctggact actgtatcca ctgtggctcg gagaacgttg 360 ttctgtggtg caaggacaac ctgtacgtgg ttaagaccct gcgggagttc cattacatcg 420 atgagaacgg ccgagaccag ggtgccagca tccgttcccg agcaaaagaa atcacctcat 480 tgctgctgga cgatgagcga ctacgaaacg agcgggccaa tcggtcctcc tggaagaagg 540 gacgacgggc tcagggagac gactacggct ctgacgagga gagtggaatt gtctcttctc 600 gaaccagaac cgccgacgac cgacgagacg accgacgaga cgaccgacga gattctcgac 660 gagattctcg acgaccctcc agacgaggat ccacgccaga cgatgaccta gcccgtgcgc 720 ttgccgagtc gcagcgaacg gccgacgagg aggcccgaat gcgacagaac ggaggagccg 780 aggacgaggc tctgagaaag gccattgctc tgtcggagga ggagcagaag aatcgagatc 840 tcggtggcgg aatggcccag ggaaccgcac agcccatcaa cctcatcgac acagacagct 900 ttcccactca gttccagcca cagcagcagc agcagcagat gcagcccact ggctatgatc 960 tgtttggcaa cgccatctac ggccagcagc agcccaccat gaacactgga gtgctccaga 1020 acgcatacgc cactggaggc atgttcgacc agcagcccaa ccagttccag cagcagcagc 1080 agcagttcca gcctcagcag cagcagtaca ccggattcca accccagcag cagccccagc 1140 aacagcagta cactggtttc cagcagcaaa acgacttctc tcagtccttc cagcagcagc 1200 agcagcagca gcctgctgag cccctccagc ctctcaaaac tggctctaac aacccctttg 1260 caaagcatga ttctacaaac ggctcttaag cttccactgg tccctccctc aacttcatgg 1320 cttctaacaa catgaccacc caggcacatc ccaaccacca acagcagcag cagcagcagc 1380 agcagcaaca gcaaccggca cccaagcagc gtgcgcaggt cactggcggt gctcatcccg 1440 aactcaacaa cctgctcatg accggagacg gagtcgacac ctttggaaac actggcgaga 1500 cccgaatccc cgcacagcac acccgagaca cgttcatgaa cgctcgtctg cagccccagg 1560 ccacagccaa caacaacccg tttgtgggcc agcagtacac cggtatcccc agcactcaga 1620 tccagcctgc agtgaccggc tacggttttg gcaacgcgcc acagcagacc ggcatgcagc 1680 agagcaacgg acagcagcag tacggacagc agcagtacgg acagcagcag aaccagcagc 1740 agtatggtca acagcagtat ggccagtttg gacagcagca gcctcagcag caacagtacg 1800 gcggaggagc accccagcaa tcgctgatag atttgtaa 1838 <210> 44 <211> 1847 <212> DNA <213> Artificial Sequence <220> <223> YALI0_D26620g <400> 44 atgtcgaagt ccatcgtccg ttccatcaag aacgtcacga acggatacac ctcggcccag 60 gtgaaggtcc gaaatggtga gtatacaatt ctcccctctc tactattgca caatcccctt 120 ctcatcacca ccgttttttt ttctcgctct actaacatct agccacatcc aacgacgctt 180 ggggtccgtc cacgtttgac cttgaggaaa tcgccagact cacccactcc aaccaggagc 240 ttttcgaggt gatggacatg atcgatcgac gactcaacga caagggcaaa aactggcgcc 300 atgtcatcaa ggccctcaat ctgctggact actgtatcca ctgtggctcg gagaacgttg 360 ttctgtggtg caaggacaac ctgtacgtgg ttaagaccct gcgggagttc cattacatcg 420 atgagaacgg ccgagaccag ggtgccagca tccgttcccg agcaaaagaa atcacctcat 480 tgctgctgga cgatgagcga ctacgaaacg agcgggccaa tcggtcctcc tggaagaagg 540 gacgacgggc tcagggagac gactacggct ctgacgagga gagtggaatt gtctcttctc 600 gaaccagaac cgccgacgac cgacgagacg accgacgaga cgaccgacga gattctcgac 660 gagattctcg acgaccctcc agacgaggat ccacgccaga cgatgaccta gcccgtgcgc 720 ttgccgagtc gcagcgaacg gccgacgagg aggcccgaat gcgacagaac ggaggagccg 780 aggacgaggc tctgagaaag gccattgctc tgtcggagga ggagcagaag aatcgagatc 840 tcggtggcgg aatggcccag ggaaccgcac agcccatcaa cctcatcgac acagacagct 900 ttcccactca gttccagcca cagcagcgat gaggacgaca gcagcagcag atgcagccca 960 ctggctatga tctgtttggc aacgccatct acggccagca gcagcccacc atgaacactg 1020 gagtgctcca gaacgcatac gccactggag gcatgttcga ccagcagccc aaccagttcc 1080 agcagcagca gcagcagttc cagcctcagc agcagcagta caccggattc caaccccagc 1140 agcagcccca gcaacagcag tacactggtt tccagcagca aaacgacttc tctcagtcct 1200 tccagcagca gcagcagcag cagcctgctg agcccctcca gcctctcaaa actggctcta 1260 acaacccctt tgcaaagcat gattctacaa acggctcttc ttccactggt ccctccctca 1320 acttcatggc ttctaacaac atgaccaccc aggcacatcc caaccaccaa cagcagcagc 1380 agcagcagca gcagcaacag caaccggcac ccaagcagcg tgcgcaggtc actggcggtg 1440 ctcatcccga actcaacaac ctgctcatga ccggagacgg agtcgacacc tttggaaaca 1500 ctggcgagac ccgaatcccc gcacagcaca cccgagacac gttcatgaac gctcgtctgc 1560 agccccaggc cacagccaac aacaacccgt ttgtgggcca gcagtacacc ggtatcccca 1620 gcactcagat ccagcctgca gtgaccggct acggttttgg caacgcgcca cagcagaccg 1680 gcatgcagca gagcaacgga cagcagcagt acggacagca gcagtacgga cagcagcaga 1740 accagcagca gtatggtcaa cagcagtatg gccagtttgg acagcagcag cctcagcagc 1800 aacagtacgg cggaggagca ccccagcaat cgctgataga tttgtaa 1847 <210> 45 <211> 2172 <212> DNA <213> Artificial Sequence <220> <223> YALI0_D27016g <400> 45 atgaaggtgg gcgatggagg agaagtcttc tttgtgttcg agaccgacgc agacgtgccc 60 gaagagctcc tgacatcccc cgtcatttct ccctcttcgt cgccatcctg gggccaggag 120 gaaggcgggg atggtgagcc ggactacctt gctctgaacg actctaaaca gggtggcgac 180 agcaagcacg gcagatcgcc ctcggagggc ccaccattca gatcaccttc ggcggatcac 240 ttacatgaga tgggcagctt cgatgatgag aatgaccctg aggtgaacag aagacaacgt 300 gcgagcacgg cagctccaga gcctgttcct ggttcgttga aacacccagc cactatctcg 360 gaaggcatct cttcggcctc gttttccaac agcgatactg atcgaacaga cacttctgga 420 cccacagaga cagaacccac agagcccaca gagcctacag agcccacaga gcccacagag 480 cctctggatc ttgagcagag tctccaccgg gctgccactt ctcccgcccc ttcgtccgag 540 gagatttggg agaaggcccg tgcactgtcc aagaaactca catcagaaaa cattcagagt 600 aaaatctccg acaacggaga cattattctg gatatgactg gttacaagta cgaccacgag 660 gacgtgagtc gatcagagga gctggtcaag aaaatcctcg ctgaggaact gggagaagac 720 agagacctgt cccacatcct ggttgaagac gaggagggta accttgtgat tcagagcgct 780 ggagacagcc accatcacga gcatatgagc tcgcccgagt ctctggccca ctcccctcag 840 cccctccctt cttctaacct tccgtctcag gcctcggaca acaagcacta cgccaagacc 900 atccgtttga cgtctgacca gctcaagtct ctggatctca agcccggcaa gaacgaggtc 960 acctttgctg tcaataacgg caagacgtcg tgttcggccc agctgttcta ctggaagtac 1020 gacattcctg ttgtcatttc cgacattgat ggcacgatca ccaagtccga tgctctgggc 1080 catctgctca ccatgatggg ccgagactgg acccacaccg gcgtggccaa gctcttttcc 1140 gatatcagag ccaacgggta taatatcatg tatctgacag cacgatcagt gggacaggca 1200 gatgcaacca gggcatatct aggcggtgtt gaccagtttg gcttcaagct gcctccagga 1260 cccgtcatct tgtcgcctga tagaaccctg gcggctctca agagagaggt gattcttaag 1320 aaacctgagg tattcaagat ggcgtgtctg cgggacatta agtcgctgtt tggcgagacc 1380 gaagacgcca ccaatccatt ctacgctgga tttggcaacc gaatcaccga cgcgttgtcg 1440 tatagatctg tcggtgtgcc gtcgtctaga atcttcacaa tcaactcgaa cgccgaggtc 1500 catatggagc tgcttgaact ggctggctac aagtcctcgt atgtccacat tgccgatctt 1560 gtcgaccact ttttccctcc ggaaagcgag ttcacgacca ttcaggagga aaaatacacg 1620 gacgtcaact actggcgaga tcccattatt gacctgtctg atctgaccga cgacgagctg 1680 actgacgatg atgagctctc caagtcgccc aagtcgccca gatctcctag aagcccgcgg 1740 gccggttcgg caggctccag cgcggctccc tcaggctcgg gcgccgaccc tgccggaccc 1800 tccgagccga aggactccgc gaacccgtcg aagttcagct ataagaaggc tcctacgaac 1860 tctcgattcc agcccgtttc gtacgatctt gatcttgacg acggatacga gtacgacgat 1920 gacgatgact atgatgacga tgaggagttt gtggacgctg agagcgacgc gctggaggag 1980 gatgacgacg atgatgatga cgtcgaccta gacaacgact ctgaccactc ccctgtcaag 2040 ccgccctcgc agatgcagcg agtcatcaac aagactattg aggacaacaa gggcctgcac 2100 atggatgagg atgacgttca aaaagccatg aaggccctga agatggaacg agcaagcatc 2160 aatcctgagt aa 2172 <210> 46 <211> 687 <212> DNA <213> Artificial Sequence <220> <223> YALI0_E07832g <400> 46 atgaagttct ccgttgccac cgtcgccgcc cttgccgccg ccgcctacgc tcaggtttcc 60 aactccaccc tcgccaccac cgaggctccc gtcacctccg ctgctcccat cgagtctgct 120 gagaacaacg gtaccgagct caccaccggc actgtctcca tcatcaagac cgagaccaac 180 gttgtcaccg cctacaccac ctactgcccc taccccaccg agattgttga gggcaacaag 240 acctacaccg tcaccgaggc caccaccctg accatcaccg actgcccctg cactcgaacc 300 actaccgttg ttaccgagac catcaccacc tgccccgtct ccgagttccc caccgagccc 360 atcactgagg gccccaagcc caccgagtcc aagcctgctc cttcccccaa gcccgaggag 420 tccaagcctg ctccttctcc caagcccgag gagtccaagc ctgctccttc tcccaagccc 480 gaggagtcta agcccgctcc caagcccccc gcttccaagc ccaccgcttc caagcccacc 540 gcttccaagc ccgagcagcc cgccgcttct actcccgtcc agacccagca gcccgctatc 600 tcctccggcc ctgagcaggc ttcttctgcc aacagggtca ccgtcggtgc cgtcgctgcc 660 attgctgccg ctgcctacct tctgtaa 687 <210> 47 <211> 1392 <212> DNA <213> Artificial Sequence <220> <223> YALI0_E08008g <400> 47 atgaagctgt ctctcgctac cattgttgcc ttctctgccc ttgttgtggc tcagaacccc 60 agtcctgaga ccgtgtccag tgatattgct accggcaccc cctccaccct gtctcctgga 120 tccacctccc aggctgcctc cggtaccctt gccgctccca ctgccacccc tacctccact 180 gccacttccg ccaacctctc tgtgctcgac aatgtgctca gcgaggttca ggccgttctg 240 gctgacgttc tggccgagct tcccgacatt ccagagctgc aggctctgat tgccaccgtg 300 aatgcctccc tgacagacat tcttgcccgt ctccctgctc tggaggcagg tcagtcggct 360 tcttcggccg ctgccgcttc ctccactgct tcagcctctc cctccagcaa cttcaccaac 420 cctctgctcg tccgacaagt tcgccgggtg gtcaagcttg ttgcccgaga ttccattgcc 480 gaactttccg acgacgtcag cactctagtg ggcaaccttc aggccattgt cgccatcatc 540 gagggcgttg agccccgatc cgactctctt caggctgccc ttgaccaaat tctggccatt 600 atcgctcgac ttgagaactg gttagctgag accaacggtg aggaggtccc cacttcctcc 660 gccggctctg ccatttccgg ctctgcctct gtcatttccg gttccgcccc tgccacctcc 720 acttctccct ttgtcaccac tctgagcatc tcttctgctt ctgcttctgc ttctgtttct 780 gctggttccg atggctcttc ctcttcctct tcctcttcct cttcctcttc ctcttcctct 840 tcctcttcgt ccactgaggt caccattgtc tacgtcaagg agtgcggctg ctacaagcac 900 aaggacggct ctgttgctac ccatgaggag gtcgaggccc acgaggctgc gcattcccag 960 tccaagggag ataccgagtc caagggagat accaagggct cctcttccgg aggtaagact 1020 acttactcca ctgactctgc ctcttctgga tccagctcct ccggttcttc ctcttccggc 1080 tccagctctt ctggatcagc ttcccacgcg ggatcttcct tctctggagc ttcctctcca 1140 ggctctgctg tttccgactc ttccagctct tctagattag ctgcccacgc tggatcttcc 1200 tcctcttccg gttcatcttc cagctcttcc cccgcagctg ctgccggcgg atcctctgcc 1260 cctggctctg ctcccgctgc cggacccgct ctttcgcctg ctggagagca cgttgcccag 1320 gccaacggtg cctctgccat gtctgtcggt accgctgctc ttgctctgcc cttcatcatg 1380 gctctctttt aa 1392 <210> 48 <211> 1121 <212> DNA <213> Artificial Sequence <220> <223> YALI0_E11363g <400> 48 atgcatgaac taaaaagatt caccatccag aagaaagaaa aaaaccacac acacacacac 60 acacacacac acaaacaaca acacacagca tgagctgctc catctgtcag caggagagca 120 aatacagatg cccagcatgt tcggcgcgca cgtgttcgct ggcatgctca aagcaacaca 180 aagcctccga gaagtgctca ggactccctg atcccacaaa gtacctgaac cgagaggctc 240 tctttacaga cacatctgtt aacagagact accggtttct caagcggctg gagcgagata 300 ttgtagtgcg caaacaggat ggcgaaagca tgccgatttt caagcgggct cgatacaacc 360 agaacagaaa agacggcaag gatggcagtg tgatggagcg aaacggagtc aagatacaca 420 aggtggctca agggatggga cggcagaagc ggaaccatag cagatgggat cctaacatca 480 agcagttttg ttggactgtc gaatgggtga atgttgacac taacgagacc atgactgtgg 540 acaaggtaaa ccctgtgcag tcacttctcg agtgtttcca gaagagaggc agtgagaagg 600 gtgggaatgg tgataataag ggtgaacgga aggtcgagaa gaacgacacc gagcaaacag 660 agatgaaaga tgaacattca aaacagaagg atggcgacaa gaaagacgaa gaaaaagaag 720 tggtcccgtt aacacctaga tcattctaca tgaagagaat caagtcgaag aacacatctc 780 caattctgtt agatccctca aagcctctgt ctgaaaatct gaaagacaaa tctgtcgtgg 840 aatatcctac catctactac accaacgaag agattgttgc gagtgacagc gatagtgaca 900 gtgacagtga ttccgacagc gacagtggtt ccgatgattc ggacgattcg gacgattcag 960 atgacgactc tgatgatgac tctgactcag ctcctgaaga ggagagtgct cgacaaccag 1020 agatgcctga cattgtcaaa acattggccg actcagctgt caacgacatg gccggcctag 1080 aggacgcctt cgaaaaagca caagaggaag gaaagcaata g 1121 <210> 49 <211> 2157 <212> DNA <213> Artificial Sequence <220> <223> YALI0_E13596g <400> 49 atggaggtgg ccatggatgg aagccacaat gggcgccgga aatgcgctcg gcgaagctcg 60 tctgccgagc tgctcgaaaa accggagtcg gaccttttga cacaaatgta ccacatgcgc 120 aacgacgact cgctggccga cgctctcacc aacaacgaaa gcgagcattc caacgtgtcc 180 acttcgtcgg cagcgtcggc gtcgaccgcc gcaacctcgg cctcgatgat ggactcccag 240 gtgtacacaa acaagcaaaa cacagccgcc aacaacgtga ccccggcgcc caataacaca 300 gacatgcggc ctcccgagtc gccgggcccg ggctcgtcgg cgtcgcgaaa acgcaaggca 360 ccaatggcgt cgtcggagga aatcgacgca gagctgcgca gagccgcaga gcaactggtg 420 ggcctgccaa ttgaagatat tgtggaaacc ctgcgtcgac aggactcgcc cgccggcaga 480 ggccatggca acgagcgcac caagcagacc tttggcatgg cctggctcat gaagttctgc 540 gagctcaatg agcagtcgtc ggtcctgcga tcccgcatct acgcccgcta cgtgaccatg 600 tgttcgcaac acaatgtgcg gcccatgaac ccggcatcgt ttggcaagct cgtgcgcgtc 660 atctaccccg acctgaccac gcgacgactg ggagttcgtg gccagagcaa gtaccattac 720 tgcggcatcc ggctcaccgg cgaggccgag gagatctcta cccccgggcc tggatcgcct 780 gatcgggcct tcacccccga attgactccc ttcgactcgc cctacgggtt ccgtggcaag 840 actgagactc ccgactacgc gctgggcctc aactccatga tggcgtccca gcccggcacg 900 ccacagcagc atatccaaaa tcatcagcag cagcatctgc aacaaaatca ccaacagcat 960 catcaacagc aacagcagca gcagcaacat caacaacatc aacaacagca gcagcaacag 1020 caacaacaac aacaacaaca gcaacagcaa cagcaacaac aacagcaaca acaacagcaa 1080 cagcaacagc aacacataat gggaaccccc caaccaggcg tacccgacct tcgtttcgtg 1140 tcgaacctaa cggaagtgtc actatcagaa ggctacgact ccctcatgct gccctcgatg 1200 gagccgtacc ttcctctggg cactgacagc gacaccgcca acacgctagc ggcgctctac 1260 aaggcccact gccagtcgct ggtcgaatcg ctacggttca tgcatatcag aaaatttctc 1320 cacattcatg gagctttcat tgggggcctg acatccacag ttcaaaagct gtatcgacat 1380 gagtcgctgc agccgtgggt ggccaaggcc gactggatta tgtacaagga catggtccag 1440 atgctgtcgc ctttggcgct gcaggagatt cctccacagg tcctgtcggg tctcaagagc 1500 ttgtcgcaat acttgcccga gtacctcaag tcgtctttgt gcaagacgtc gccggggttt 1560 gtggagacca agctcaagcc cgcgcgggcg tttgcatcac tgctgatgcg gctggtgcgg 1620 gtgtcggagc tggctctcaa cgccggcaag ctgctggcgt ccgaccacga ccgagcggtg 1680 atgaaaaacg attggatcaa gtacgtggac agcgggctga tagtggctcg agacgttccg 1740 tgctcatcac aggaggtgac caagattctc gacgtcgacc ttccgcagct gctggactac 1800 aaggatccgg tggcgtcggc tatagtggag cactgggcaa tgtacctgat gactcttccg 1860 tcgcggttca ccaacgtgta cccccatatg tttctgcttt acatgaacgc tgtgtttaca 1920 gccgcactac gggagatttt tctcaacgga ggagacgggt ttggagcgtg gatggtggtt 1980 cggtgctggg tggatgaatg gatgggatgg acggccgaac agggcacgtt tctgtctgcc 2040 gaccacaaca aggaggtcaa caagcatgag aacaaggaag ggacgcccca tggcaacccc 2100 gccaagtttc tcgacatgac tgtggggttg ctcaagccca cccacaagga tgcatag 2157 <210> 50 <211> 4466 <212> DNA <213> Artificial Sequence <220> <223> YALI0_E14388g1 <400> 50 atgaccggcc ttgcagagga atggtggttc gacagtggta gggccgctct gggcgcagac 60 gccagagatt ggaagaaatt cacccaggcc ctcgagagcc gattcactcc ctcgacttac 120 gccatgaaca tggaggagga ctggagcaat ctgcaatgtg ggcccaacga gtcatcctat 180 tcctatgaga cacgcttccg agcggcacga caccacctgt ccagagaggc cgccccagag 240 gaggcctggt tacggctgac cagtggtatg aaggacatgt ggcgacgtga gatcatgaag 300 cagggcccca tactgcagaa caacgtcgag gagaccctgc gtcatatgca cattgtggca 360 ggcgcagaat acttcccagg agctcacgcc cagacacccg cggccgcacc tgtggccaca 420 cctacatcca ccagccagta cggtcctgga ccgatggagg tggacaacgc ggtgctgctc 480 aaggctatga tggaatcgca ggaggccagc cagaagcgaa tgatggaaca gcagacccat 540 atggttgaac tgatggcaaa cgccattgga cgagctaaca acaacggctc ccaaaacggc 600 cgcaacaacc gtggtggccg tggtaactac tacaaccagc gaacctctcg tgagtgctac 660 acctgcggca agattggcca cctggctcgt gactgccgac atcgtgaacg tgttcaggaa 720 ctgattcaac aggaccaggg aaatgtgcga cagcagtaat gaccgagacc attactgctg 780 agccactcga agatctcgaa tcggtgagtg tgccacctgc ggagtatgat ataatcaata 840 aatccaactt tgtagaggac actaccggag aaccggagga tgcaggggta gaggtgcatc 900 tggaggaaga gcaaacggag gaaccaatgc agctggagga agaacaagtg ggggaacaag 960 tggaggaacc actgattgct gcacgagttg aggtggtaga tggtaagcgg cgacgagcct 1020 tgatcaaggg cgaccgacca ccgatctcga tcccgtactg ctacaacggt accacagtgc 1080 aggcgttgtt ggactgtggc gcgagctcgt gcttcattag caggaacctg gctgagaaat 1140 taggcctgaa aatgacgccc tgcaaaccac gacaagtcca gtccgtgcat agcatggaga 1200 caaccaacta tactgtggaa gtaccggttg agctgggtaa gtggggttgc gacgtgtttg 1260 cgtacgtctt accccagatg gtcggacaag agctactatt aggcatgccc ttcttcgaag 1320 agtatcatga agccgtggat ttcaaagctc gaacgttcac acccgacggg tacgaggtac 1380 cggcatggcc agctaacgag tcaactacgt gggacaaaca cggccacatc aagtcgtgct 1440 cactagagaa agctacgcag ctggccgaac accacggtgc gcagctgttc ctctacatgg 1500 tacgggagaa gccagagggg gaggagcatg agccggatgt cgatacccga gaggtgctgg 1560 aggagtatgc agatgtgatt gttgacaaaa tgccaatgga gctacctccg aaacggagcg 1620 tagaacatac catcgactcc gatgagacgg cacgacctcc agccagggca tcgtatcgac 1680 ttacccgatt cgaatgggct gaggtcgaca agcaggtaaa cgacttgttg gagcgaggaa 1740 tcatccgacc atcgaagtca ccttacagcg caccgttagt gattgtaaaa aagaaaggag 1800 gtgaacttcg tatctgcaca gactatcgcg ccctcaacga gcttaccacc aaggatcgat 1860 ttccgctgcc ccgtatcgat gacattctgg attgccttga cggcgccgac accttctcca 1920 agttcgatct tttgtcgggc tactggcagg tgttggtaaa agagtctgat gtacacaaga 1980 cggccttctc gactcgatcg ggacattatg agtacctggt aatgccgttc ggtctatgca 2040 acgctcccgc caccttccag agattgatga atgacgccct acgaccattc cttaacaaga 2100 cagtctgtgt gtatcttgac gacatcatcg tgttcagccg aaaccgagag gaccacaaac 2160 gacacgtgag ggaagtcttg gacgccctga gagcacagaa gttctatgcc aagaagtcga 2220 aatgtgagtt attcaggaag aagatgggat tcctgggcca cgtggtgtct gcggcaggag 2280 tggagccaga ccctgagaaa gtgaaggttg tggaagagtg ggtacctccc aacacgccga 2340 aaggcctctt gagctttcta ggactgactg ggtattatcg acggttcatc gaagactacg 2400 ctaaaatcgc cgccccactc acagacgctg ccacattgtc gccaactgac tttaaatgga 2460 ctgaggcatg ccaggtggcg tttgaacaga tgaaggcgaa gctagtatcg aacgaggtca 2520 tgatcatacc gacgatggag gacacgttca aggtgagcac ggacgcgtgc gatattgcga 2580 tgggcggagt actacagcag tggagtccca aggaccaaga gttccgacct gtggcatatg 2640 agtccacgaa gttcaagaaa cacgagatga actacccgac gcgtgagaag gaattctatg 2700 ctatcatcca tgccctacgg aagtggcgac actacctact tggccgaccg ttcttgattg 2760 agacagatca tcagtctctg agttacttta cgtcccagac acaccctccc agtggacgac 2820 tgagtcgatg gctcgacttc ctagcggaat acgactttga gatcaagtac gtgccgggca 2880 aagacaacga cgcagccgac gggttgtctc gtatgttggc acagacagcg atggtgttcg 2940 agccggacga ctcgttgcta gacatcatca agcagggcta tgagtcggac gagtacttta 3000 aggacgtctt caaggtactg gccacggaac cggtggtcat ccccaaagag atgcacaacc 3060 acgcccgcca tttccgatac gacaagaaca caggactgct gtatttcgcc tccgtttaca 3120 agggggaggg agagcgactc tgcgtgccaa gaggtaaggc cagaaaaatg ctgatgaagg 3180 aggctcatga cgcacccctt gctggtcact acggatactt caaaagctat gaaaggctgg 3240 cgagggctta ctactggcca cgaatgatag accacatgcg caaccacacc agatcctgtc 3300 tgatctgcca gaccacgaag gccagacgag caccacccca gggcttgctg aagcagctgc 3360 cggtaccgac gggaaattgg caggagatca caatggactt cattggaggc atcccaacga 3420 cccatcgagg ccacaacaat atctgggtca cggttgatag gatgtcaaag atggtacatc 3480 tgattccttg caagacgagc acggatggag aagagttggc ggacatgtac attgaccgca 3540 ttgttcgata tcacggagta cctcgttcga tcgtgtcgga cagggacaag ctattcacag 3600 caaagctatg gcagacgtta cagacacgtc taggcacaga gttgaagttt tccacagtca 3660 atcaccccca gactgatgga cagtcagaac gagtgaacac cgagctgatt cgacagatga 3720 agcaacactt cgtcacggac aaaaactggg acctctggtt acctgtcatc gagtttgcga 3780 tgaattctgc aaagcattcg tccaccggat acgcaccctt cgaggcggtg tatggataca 3840 tcccagacgg accaacgtat gcctcgactc gagagctcac caaggtacac catcaaatgg 3900 acgcttggat ggacaaacta cgggctattt ccaattcgat gcacgaccga ttaatcgagc 3960 accaacgggt ccaggagaac agggtgaatc agcatcgagt gccagtcgcg tttcagatta 4020 acgaccaagt cttggtacat cggaaggcgt tctttgacaa ggccaagtac gcaaagatgt 4080 atgacgtcta ctttggacca tttcccatcg agaagaagat tgacaccaat gtctacaagg 4140 tgcagctacc atacgactcg actcgacaca agaatatcaa tgtacaacac ttgaagaagt 4200 tcatacctcg acctgagtat gacatcaacc cacccagtac ggagtactct caagaatgca 4260 gcctgcatca gatcaccagc ctggtgggta ttgatgacga ccgttacttc gtaacttggg 4320 aagattgtga cccctctatt gcctcttcaa tttcgaaaga gatgttccac cgcatcccta 4380 aagacaaacg ggattcacta ttggatcaat ggaaccagtt cattaagacc cctgcggaaa 4440 gcgaggacta tgtggacatc tcctag 4466 <210> 51 <211> 3672 <212> DNA <213> Artificial Sequence <220> <223> YALI0_E16731g <400> 51 atgaatacat ctgcagaagg agcagagcag ggcaggcaag atccccactg gacgtcgagg 60 acgaggatct ttggcatgga ggacgggtcc gagttctgga gtgggtcaac cctcacgccc 120 accaaacata ttgacaaggt ggtctttgga agcaacgcgg tctggcgcgc gtacgcaaag 180 agccatggaa aggttctctt ggttgatctg agctccggac gccaggatgg ccgggagttc 240 caacattgtg tgagctttgt tgcaaaggac gaagacggcg acaatgccta cctcgcgttt 300 ggatacatgc gaaacaagac gattgaggac ttcatgtggc tgtttgatat gatcaaggtc 360 cagttcaaac accactcggt gcctcttccc aagaccatct actccagcga gatgaaggag 420 tgctacgaga ccatcgaccg agcattcccc aacagtaaca tgcggttgtg gcttcgagag 480 atcaatcggg actttgtagc gcagtgccag catctggtgg tgcgtgactc tgatcgagtg 540 cttcacgaca aggtggatcc ttccagtttg accaaggacg aaaacatatg gcttgaaatg 600 atgtccgact ggtcggagat tctggaatca gacagcaaga accatttcaa ggaacaatgg 660 gacgagttcc gcgccactta caagaagagc caccccgaag ttgtttcgta tctcagaaac 720 caatggatca agccgcacaa gcgcatcttg acgggcttgt ggacaaacaa tgaagaggga 780 catgtgtttg atcagaatgc atacatggag ggtgctgcaa agtttttgga gagagccgcg 840 ggctgtcatg gcctggaaga ccgtctggta gctattcaga ctgcctacag agaaaatcat 900 cttactgagg gcagctctgg aaagcgacca agcgatgagg atgagtatca ggaggatgtt 960 gatgatgcaa caagtagaat tatgagcatt cttcgtggag gagagaacga ggagaagtcc 1020 ggaatctctg aggactctga ggaagtggaa gtagtggagg atggtgagga agtggacgag 1080 ggtgagaagc aggaggtttc tgaaaaggat acatcggtag aggaggagga ttctgaagtg 1140 aaagaggagg aagagcatga ggaggcagaa atggaggtat ctgaggtggg ttctgaagag 1200 gaggactctg aagaagactc tgcagatgcc tacgaagagg agaggactga cgagtcgggc 1260 gagagtgatg ttgaagacac aaagaaatct gcaactcggg ctgtgaaaac tgtgaaccac 1320 ccgactactc ctcgaaaagc tactgtacaa gcaggcgctc caaccttgat aggtcacctc 1380 cccctcgcac tggtttctcc tacagccacg actttgatgc ctcccaggat gacaccactt 1440 ggagaggtat tccttcacgt tccgggaatt ggctatttgc ctgaatggcg tgctctagag 1500 ttacagaaag aatttgaaga agctaagaga aagcaggatg cggagagaga ggaagatgtg 1560 gtgatgttgg atgtccatga cttggaaaag gaaaaagagg aggaagagga ggatgaggag 1620 gatgaggagg aagagtagga tgaggagtaa gaggaggaag aggaggaaga ggaagaggaa 1680 gaaaactctg gctttgatcc tgattctggt aaacccttgt cagagagtga aagggagcga 1740 ataaaggcgg tcagaagaaa tttgcgtctg gaaggatccg gtacggaact tgaaaggttg 1800 ctaaattcta acaagcgttc aagaagtggg agatcacgtc catctctgga tggaaccagg 1860 aagagcatta ctgagatccc taatgaaggg aaaggcggtg ttgcatccga tacttttgtt 1920 gcagatgtcg acgggtcgtc agccagcaaa cgatcgagaa gaggtcagca gcgagcgtct 1980 tctacggttg ctacgctaaa gacgcccaaa cccaagaaca agactcaaga aaagagtgct 2040 gagtcggagc agcctgtaaa gcggggacca ggaaggccac gaaaacaccc tctcggtaag 2100 gagcctaagg cggctgtaat aaagcgggga ccacgagggc cgtataaaaa acgtgtcctg 2160 tccgcagcta cggtttatga ttctgattcc ggggatgctg ccgagccgaa gacgtctgga 2220 atgaaaagct ctgacactcc tacgaaatcc agaggtcgaa cgcgagctgc acgacgttca 2280 acgatggttt ctgaggatga ggaagagaag gacagggaga agaaagattc agtgagagat 2340 tctcctgcag atacagtgag ctcttcttct gaggacgagc ctgtggtggc agttccaaag 2400 aagagtccaa ggagtatcag cgtcaacccc accaccaagc ctaccgcgaa gaagactact 2460 gcagcagcct ctaattccaa gtcatctccc acccagacta gaactcgcag atcgatggcg 2520 tcaaaggaga actctacagg agttttaaca gaaaccccca ccgcagtttc caagacggtg 2580 actccacgag ttgctagtgc aaagtcgaca ccgatacaaa ccagccagtc tcccatccag 2640 actagaactc gcagatcgat ggcgtcaaag gagaactcta caggagtttc aacagaaacc 2700 cccaccgcag tttccaagac ggtgactcca cgagttgcta gtgcaaaatc gacaccgata 2760 caaaccagcc agtctcccat ccagactaga actcgcagat cgatggcgtc aaaggagaac 2820 tctacaggag tttcaaaaga aacccccacc gcagtttcca agacggtgac tccacgagtt 2880 gctagtgcaa agtcgacacc gatacaaact agccagtctc ccatccagac tagaactcgc 2940 agatcgatgg cgtcaaagga gaactctaca ggagtttcaa aagaaacccc caccgcagtt 3000 tccaagacgg tgactccacg agttgctagt gcaaagtcga caccgataca aactagccag 3060 tctcccatcc agactagaac tcgcagatcg atggcgtcaa aggagaactc tacaggagtt 3120 tcaaaagaaa cccccaccgc agtttccaag acggtgactc cacgagttgc tagtgcaaag 3180 tcgacaccga tacaaaccag ccagcccaca tcaaactcga cctcaggcac ttcgagttcc 3240 aagcagaatc ggttgtcttt tgcttccaag tcgactccag ggcccaagag gaccccagag 3300 aatgcacctt cgaattcgac tccaaagtcg tcttcgcaga cttcttcgaa gacagtcact 3360 gagtcaccta ctcagaagac tacaccaagg ccagcttcca aggcgacgtc caaggcgacg 3420 tccaaggcga cgtccaacgg cactccgaag tcagtctcct ccaagacgac ccccaaatca 3480 accccgtcag attccatgtc tcggaaacga ccgactgagg aggctggatc tgggtctggc 3540 cggaagggca agaagcagcg caagtctgat gtcactcaga acggagtgtc caaggccgat 3600 acacccacca agagaaaaaa cagccagtct cgtccttcgc tgggtcagag aattttggga 3660 ttcttcaaat ag 3672 <210> 52 <211> 933 <212> DNA <213> Artificial Sequence <220> <223> YALI0_E18073g <400> 52 atgcagttca agaacattgc cctcgttgcc tttctcggtt ttgttgccgc ctccgagtcc 60 gttcccgagt ctgtggacga cactcccgtg aacctcgacg atctgacagc cgaggaggct 120 gccttcttca tggacaagct caagaacaag tgtccgatga tgaagcagcc ccaggagtcg 180 tgccctaagc agtcgtgtcc caagcagcgt cagcagtcgt gtcccgcttg ccccaagcag 240 cagtcttgcc ccaagcagca gtcttgcccc acgcagcagt cttgtcctaa gcagcagacc 300 aagacggtca caaagaccat gagcaagcag ggagcggccc agaccaagac cgagacctct 360 acccaggtcc agttccagac tctgaccgag actcagaccc tgtatgagac tcagtggcag 420 actcagtggc agaccgagta ccagacccaa taccagactc tgtacgacac ccagtggcag 480 actcagacgg ttcaggggcc cactcagtac gtgaccgaga cccggactgt tcagggcgct 540 cctcagtacg tgactgagac tcagacggtt cagggtccca ctcagtacca gactgagtat 600 gtcaccaaga ccacccaggg agctcctcag tacatcaccg tcagtggagc tcctcagtac 660 cacaccgtca ccaagactgt tcagggccag aaccagtacg tcaccaagac tgttcagggc 720 cagaacaagt acatcaccaa gactgtgcag ggccagaaca agtacgtcac caagacggtg 780 caggcgcccg cccagaccat cacccagacg gtccgtggtg ctccttcgct ggtcaaggtt 840 cctgagtatg ttactgtcac ctccactgtc actgttggca agaagcacca gcaccagccc 900 aagaaggatt gcaagaaccg aaactgccag tga 933 <210> 53 <211> 960 <212> DNA <213> Artificial Sequence <220> <223> YALI0_E18216g <400> 53 atgaagttgg aaaacatttt gaatgaggca tcaagagaaa agtccgaggt tccaggtccg 60 gatgccactc aggctgagga cgggtccaag cgcgacggca agctgcctgc acacgaccac 120 cacccccaac gcagaccctc ggtccattcg cccaccagca ccagtacagc tgccggccag 180 gtggttgcac cgagtggaca acagggacca agtggaccta gtgggccgag cgtacaagcg 240 ccgagtggcg cacaaaccac gtccaaccag attgcggctc ccggacaaat gtctcgtcct 300 ccgagcacgg gccactttgg aatgcagccg gttccacaac cacacgaatt cattccgggg 360 ccattgcacg cgccccagcc ctattatccg catcatctac aacaccaggg ccactttccg 420 cccctgcaac cgatccagat cccccagtgg accgctccgc accacggcca tcatcccagt 480 ctgacccctg ggccctcgcc gaccgccacg gcccctgaac atcgtcacag acgcaactcg 540 tcggtctact cgccgtatca gcggcctcgt ggcggctccg tgggccatct ggggcccgtg 600 cccaccacca gctcacattt gccgcctcca gccacagtca cgtttgctcg gggcggcttt 660 gaagacaaga ccagcgacgg cagccaacgg aaacgcagca aggcgcccaa cagcacgtgg 720 accatggagg acgacaaccg gctgctggac ctggtgctgg ccacgttacc gcggcaggac 780 tacgccgagt acgcccgtct gctcaacaaa cgggacagtc agacggtgcg gtaccggtgg 840 aaggtgttgg ttcgacgggc acggggcgag ggagagcagc aacagcagca gcaacaacag 900 caacaacagc aacagcaaca gcaacagcag cagcagcagc agcagcagca ggacaggtag 960 960 <210> 54 <211> 1470 <212> DNA <213> Artificial Sequence <220> <223> YALI0_E20449g <400> 54 atggatctgg cgaaaatcac cgacggcttc gtcaagcacg agacctcgtc gtcgtcctct 60 tcttgctcca ccaccaacac agggcccacc ccagacttgt ctccagtgac gccctccaag 120 gaatgtgaga agcggccacg agaggacgac cctgaagagt cgcacgacac gagcgccggc 180 gccaacagca acaacaacgc tagcgtgtct ctcatgtcca ccccagagcc caagtcgtcg 240 tctccccccg gactgtcgca tttcgcacac ctgatgcaaa agtcggacac catgtaccga 300 cagaacctca actcggacca gtacatctac tcggacgagg agaaggagaa ccacaagact 360 tcgggcaagc cccacacccc ccaggtgcct catacgccct ccagtgtgcc gacacaacaa 420 ccccaatatg catttatttc acattccatc acctcgtacc cgtcgaacga gcctcagatt 480 gacaacgcac ggctggcgcg ccgaaaacga cgccgaacgt ctcccacgga actcgcgctg 540 ctggagcagg agtttgcccg caaccagaag cctcccaagc acattcgcgt cgacattgcc 600 cgccgagtcg acatgactga aaaggctgtg caggtgtggt tccagaacaa gcggcagagc 660 gtgcgaaaga gcatgaacaa gagcatgacc gatgacacct ctttcgccga ctcttcgttc 720 gctgaaacta cctttgacga gacagacggt aactccacat tcctgtccaa ttccaacgtc 780 agcaccagcg taagcaacaa gtcaatcact tcttccatca cagacaacaa gtcgcccctg 840 gcacagtcaa ccaccgccga ctctgttgcc aacgccaacg ccaacgccaa cgccaacgca 900 aacgccaaca acaacaccgc atccacttcc tccacaaacg actccgaaat tgcatccgtc 960 gcccccaaaa caaacggcag ctcattctct gttttcgaag atacccccga gactcccgcg 1020 aaaaagaaac ccagtgctcc gcgactgtcc atgcgtggtg ggaaggctac tgttatctac 1080 gccggcaagc ccaagggtgt cacgctgtcc tcgggaagac gtcttggggt ccctgccaca 1140 ccctcctctc ccgccaacaa caatcttggc ctgggaggct cgcctctggc cacatcgtct 1200 cctatgaccc agcggaccgc gtcgcaactg aaccaggcat ctgcatcttc tcccctatcg 1260 gctgttaagt ccaagtcttt tggaactgcc gaggaaagcc tggctgcgac gctcaagaag 1320 cggcttccgt ccatgcacta cgacctgccc gtgaccaaca agacgtcgtc tgtgcgccat 1380 ggcgtgagct ctcccgtggt cgacgccggc agccgtgagg ccgagtgtat ttccaatctc 1440 ctctctcttc gaaacggagg acgatggtaa 1470 <210> 55 <211> 1185 <212> DNA <213> Artificial Sequence <220> <223> YALI0_E21109g <400> 55 atggcccccg ccgttactgt caccgctggc caggaacctg ctcctgcccc cgctccccag 60 cccgagccta cccccgctcc tgctcccgcc cctgctcctt cccctgcccc tgctcctgga 120 cctgaatctc aggttcctcc tgctcctgct cctcaaccta ctgagcccgc ccctgcccct 180 cagcccaacc ccgttgagtc ctctcctgct ccccagccca cttcccaagc cccgcctgcc 240 cctcctgccc ctcctgcccc tcagactacc tctgacgctg ccccctctcc ccagcctgcc 300 cctaccacct tttccacttc tgttatcgag ccctcttcca cctcttcttc tgctgcggag 360 gccactccca ctttcacgcc caacggaatt ctgccctact cgctcaccta ctcgccctac 420 aacgacgact cgtcctgcaa gactatggac gaggtcatga aggacctcaa ggagatcgtc 480 gccaagggta tcaaggtgat ccgtgtctac ggcactgact gcggctctgt ccagaccatt 540 gaacctgccg ctaagcagct tggtctcaag atcaaccagg gtttctggat cggaccagac 600 ggagtcgact ccattgactc tggtgtccag gagttcatta actgggtcca gcagaaccag 660 gcctggggta tgattgactc tatcactgtt ggaaatgagg ctatcattgc cggctacgtc 720 agcccccagc aactccttgg aaagatcggc caggtcaaga gtcagctcaa ggctgctgga 780 taccagggac aggtcaccac cgccgagcct gctgtctcct acaccaccca ccccgagctg 840 tgcactggcc ctgagctcga ctacgttggg atcaactctc acgcctactt caacccccaa 900 cagtctcctg agaccgccgg ccagtttgct ctggacgaga tggctcttac tcagaagact 960 tgtaacaaca aggttgtttt tgtcaccgag acaggctacc cttctgctgg taacaccaac 1020 ggcaacaatg ttcctactcc ccagaaccag gaaattgcca tcaactcttt gctcaaggcc 1080 ctcaatggct acggcacctt tttcaccatg tacaacgact tctggaaggc ccccggtcct 1140 tacaacgttg agcagcattt tggtatcatt aacattcttc agtaa 1185 <210> 56 <211> 1146 <212> DNA <213> Artificial Sequence <220> <223> YALI0_E23969g <400> 56 atggtcgccg caaaaatcgt cttttccaca gcgctgctgg cgctagtggc cggcgccgcc 60 accgatgcaa cctccaacaa cgttgcagac ctcatgcaga aaattgtcga gaaggacgct 120 gtccccacca tcacagacca cagagagccc ccgttcgtgg ttgttgagaa acgacaattc 180 cagggcaacc agggtaacca gggtaaccag ggtaaccagg gaaatcaggg aaatcagggc 240 aaccagcaga acaacgccac ctcctccatc cccaccaact cgggctcttc ccaggcagga 300 ggcttttctt ctagctcatc cccgggcttc ttttcatcca cggagatttc ttccaccgag 360 tctagctctt tcgccgagtc cacttcctcc gagtcctcct cccggtttgt gccctcatcc 420 acctcgacct cctcggagag ctccagctct gagatctcta cgtcgacctc ctctgagagc 480 tcctctaccc ccgagtcctc taccaccgag tcctctacca ccgagtcctc caccaccgag 540 tcctccacca ccgagccctc ctccaccacc gagtcctcaa ccactgagag ctccacgtct 600 gcatttgtcc ccgtgcctac cacggagtca tcctctgagg cttcttcctc tgcttctccc 660 tctcctacct cgacagagtc ctcggcctcg ccttctccct ccaccacaga aggcccctcg 720 tcagagtctt ccactgaagt cacttccgac gccgtggtgt cgtccaccct aatcgacgcg 780 tccggcggcc gaaccaccgt tatcatgacc ctcacaaaca caaaggtgac ctacgtcggg 840 tccacaacca ctctgcctgt ggagaccccc accgcaagcg gagtcactgg caacaacaac 900 aagtctacgg gctcgacctc ccacacgggc cgaaacgtcg gtatcggtgt gggagtgggt 960 gttggtgtac ctgttgtggc agccgtcatt ggaggtctgt ggtggtggcg acgaaagcga 1020 ggaggagaca cccccatgta cgacaaggac gatctcatca gcggaggcga ggactccaac 1080 gtgtttcgat ccaacattga ccagtaccat gcgcctaacc gggccaactc cgcagcgaac 1140 ttttaa 1146 <210> 57 <211> 3228 <212> DNA <213> Artificial Sequence <220> <223> YALI0_F04906g <400> 57 atgcagtgtg aacccaatcc gatatttttg ttatcttcct ccacacaact cagctctgga 60 atggcatttt ctggctggca gaacagtccc ggtgggcatt ccgtcggcgc caagctggtg 120 gctcgtgccc agaacgaaaa cgaacaggtg gtgcccggca cggtcttttt gtcgtaccat 180 ggcgagggtc agccctgggg ccggaccctg tctgacgcca acgagaagat tagcaccaag 240 cggttggacg acattgatcg gatttgcatt gagatgattc tcaacattga aaggacacgt 300 ggcccgattg ttcccagcct tcaggagtct cctacgagct gttccgtgga actgactcgg 360 aaggagctgg accacatgat cgagactcct gctttcgagc tggaccgtcc cacggtcgac 420 agcgactggg gcggcaagtt cccacgtgcg agagcgctgg aaattgaggc actgttatac 480 aatgagttct tcaaggagaa ccaacacagt gtgacctctc ctgcacgcag acgtcgtgac 540 caaaagtaca gacaaaagta ctaccagccg tttgtggacc ccggcgtggg ccccagggag 600 acagacaggg agtacagaaa gtttcttgcc gctcagcaga gggatgccga gaccagaggc 660 aagcccgaga gggaagagga cagcaaagga gaaggactgt tttccgacaa gttttattct 720 gccaacgata tcgaggagga ggctcagcgc gacaacgaca ccgaagatgc agttactggt 780 actatctgga gcgaaaacga gaaggaacac ttcttctctc ttctaggacg agtggggaga 840 caccgactgg gcgagattgc cgacgcaatg gactccaagt cacttgccga ggtcgaggag 900 taccacgatc tgctgtttac cgcgtctgag caccagaagg aaatgttcaa tgtccgagta 960 aatgctgggg aggagttcat tccggaaaaa gaccgaccgg tttcgttcaa ggagatccca 1020 gctgcttgtg aaatgtccga caagtggatc gctctggagg agtcgtttgc tcacggtatc 1080 gccaagtggg aagatgagca tatggctaaa ggggaggagc cgttttgttc gatgagagtt 1140 ggggacgaga cgaaagagaa cgcgtctgag gacttactca aaaccgaggc gttggtgtct 1200 cttgctgcag aaatcttcta ccccaatcct gccaatggta tggatattca aagcaaggag 1260 tacaacactg acttgcctgt gtttgagggt attgagggag atgcaatcaa ggagttgttg 1320 attagaatcg tcgacaagac ccgcgaatta ttccgcaact acatggaagt tgataaaact 1380 acgtatgctc gaacagacag caccatgtct accggaaata tgctgtttgt tcaagggctc 1440 aggtacaaca aattgtctcg tttcttccgg agctactggg agagaagtgg atctgagtac 1500 gtggatgaga gatcggaggt tccgttcccg gagtcacttg ttacacccgg aacaactgaa 1560 gaacagcaca agaagatttt cggagaaatc aaggatgccg cacttccaaa gctgtacaca 1620 aacacaggtc tgcttgctgc gagtgtgaag agcatgtcca agctggacaa ggtcaaattt 1680 ttggacgtca aggagcagga gaggttgaat ggtatgattg aggaggaaaa aaagtacaag 1740 cgtgaacttg aggagggaaa tctagagtgg gatttcaccc ctgaagcacc aaagaaaccg 1800 tacaaaaccc gttccgaaag gaatctatcc gagattctca tggaaaagag agaccaagtt 1860 gataccgatt acttgtaccc cttcaagagc gcccatgtcc ccgtgtttct cgatattatg 1920 cacatggagg agaccggtat gcttgagaat ttggatgagc gcaattctaa gattatggag 1980 gccggtctga ctcgcttgct tgcaggtgct gctccataca cataccccag gatcactact 2040 caggttaaca agctcatgta tgagcaggac aagaagctgg aggaaagatt caaggagaag 2100 atgctgcggt acaaatacga cggcgagggg aaggagatca atatgtggaa gcaggatctg 2160 cctcatgtga gggaggagat gataagacga ccacaagacg tcctctggaa ggctagagat 2220 gaatatgttt acggtctccc ctggtataac cccggtacga gaggtcccgg aggcggtttg 2280 aaccagccag aaaactctta ccctcctagt gttccgtatc cttcttctga tgatcctgtc 2340 caggatagga caagatacat atatcctgac ggatccttat acgacgaata tagccgacag 2400 gtattccagt ttcgccgcgc gcgcccgttc tttgagctca cccattctcc ctacattgag 2460 attggcaggg gtatgctgag tgagtggatg gaccatgatg atattgagaa gggaactcgg 2520 gaccatgtga tgggcatgag gcaatacttc cacaacttta ccgacacacc gccaactaac 2580 ccgtactctg tgtactacga ggaggagcgt ccgaaatatg aaactgcaga ggatgcggaa 2640 tatgagattg aagatgagaa agatgcagcc gctgaccccc ctcttgctgt gttgggggtg 2700 gaggaggaga agaaggagga ggagaagaag gaggaggatg aggtggtgga acagtcgccg 2760 ccgccgaaac acttgtcctt gtatctttcg aagcagcagc gacaagatat gcaggaacgg 2820 atgcggcagg agtatcagga tgtggtggat aagctggcac agagtggata cgaaaacgtg 2880 gagagatttg gcatatcgcc gttcgatctt aaccccattt atggccactc tggtttgttg 2940 cttcaatatg acaccctgaa cttccgatcc gacgatattc ctggtcggtt catggtgcag 3000 cagcgggaat catacaatca aatgcatatc cggcagccac aagacaatga ctatgtggac 3060 cctaacgaat acacaccggg ccgtggtcta aaacgcaaag ctcgtaacca cgaggaggac 3120 acaaggccgt ctaagatgcc caggttggaa ggcagcgagg tgcggaggct ggaggagatg 3180 tccatgacga acaatggatc ggaggatcct gtttgcagag ttagttag 3228 <210> 58 <211> 2424 <212> DNA <213> Artificial Sequence <220> <223> YALI0_F12221g <400> 58 atgactgcgc gagcgccccc tgccaaccgc aagctcagca acatgctgat ggcggacttc 60 aagcagtgtc tgactctgta cgaaaaaaag gagtacgccg aagccatcaa acgggcggat 120 cacattctga ctcagaagcc ggatcatggc gagactctgg cggtcaaggg tctgtgtcgg 180 taccatcagg actcgcccgt ggagggccga tcgctgatca acgccggaat gaagaacgac 240 cccgagtcct acatcatctg gcacgtgctg gctcttctgg agcggtcgga aaaaaactac 300 cacgcggccc tcaaggcgta ccaaaagtcg ctgaccatcg acccgtccaa ccagaacgtg 360 ctgcgggacc tgtcgttgat gcagatccag acccgtgagt acgagccgct ggtgatcacc 420 cgacggaaac aactgaccga caagccggag atccgggcca attggaccgg gctggcggtc 480 gcgctgtttc tcaagggcga ctacgccacc gccgcacgaa ccctcgcggc atacgaggaa 540 agcgcccccg aaaaggaacc caaggacaag acccagtcgg acgacattga aaactgcgag 600 ctggcgctgt tccgaaacaa gtgcctgatc gaggccaagg aatacgactt ggcgctcaag 660 gatctcgagg agctggaaaa taaaacctcc aagcgccata tccccgacca gcagtccatt 720 ctcgaacatc gatgcgacat ttacatccga cagggcaaaa ccaaggacgc ccagagactc 780 acccgagtgc tgctgcgacg aaacccccat gaccgggctc tctaccgaca gctggagaag 840 gtgctcggca tccaggacaa caaccagctc aagtctgtca tgtacaagaa gctggctcaa 900 aaataccccc ggtccgactg tcctcgatcc atgcctctgg agttcctcga gggccaggcg 960 ttcgacgagg ctgccgacgc ctacgtgtcc accctcatcc atcgacgggt cccctcggcc 1020 ttcatgaacg tcaagcacct gtacaagaac cccgccaagg ctctttctat cggcgccatt 1080 gcccaacgga tctttgatgg cgccactgaa gatctttctg gcgcctcgga ttttctgtgg 1140 tccgcctgct ttctggccca gcacttttcc aagctcggcg accagaacaa ggccctcaag 1200 ttcattgaca atgccattga gcacacccca accctggtgg agttgcatct gacccgagcc 1260 aaaattctca agcgaatggg cgcggtcaac aagggtgctg aggccgccaa tgccgcccgg 1320 gaactcgatc tccaggaccg ttatctcaac accaaggccg ccaagtacac tttgcgggcc 1380 aacaacgcgg acgacattga gcaggccatc aagctcatct ccatgttcac gcgaaatgac 1440 acctccggta ccggcgttca ggacctgcac gatatgcagg gctactggtt cctgtccgag 1500 ctggcccgac gacagcgacg aaacggagac ggccccttgg ctctcaagcg acacaaggcc 1560 gcgctcaacg tgttcagcga gtttgaacac gagcagctgg acttccatct gtacgcgctt 1620 cgaaagggaa ccatgaacgc gtacattgac atgctgcagt gggaggacaa gctgtacaag 1680 agtaagaagt ttatccggtc cacccggggt atcattgaga cttacattgc gctgtgggat 1740 accaagcccg actgtggctg tggagcagtg attccgggct ctttccctct taaggcggtc 1800 gactacaacg cctccaccac ggtcaagaag aagaagaaga agaccaacga gggttctgcc 1860 gaggccaaac agaaccctaa cggtctgcct acccccgaac atcgacacga tgccgatccc 1920 gagggtctca agctcgtcaa gaacgtcgat ctgctggaaa ctgcccttgt tcatgcgaaa 1980 aagctcgccg aggtcggcga cagcctcggg cttgtgttcc aggccgagat ttatctgcga 2040 cagaacaagt acatgcttgc tcttcagtcg ctcaacaagc tcaaggcctc cgacgccgca 2100 tctccctacg cctctgtgct ggccaaggtc gccgaggccc agcttaatgg cgatgatagc 2160 accgcccaga acctcaagga tatgactctc aagatgcttc ccaagctgtt caacgagaca 2220 gagtccgatg ccgtggccac cgctcgactg gccttttccg tgccctctta caaggagcta 2280 gctggagaat tgctcaaggg tctggtctcg gagaatgcca ccgctgccgc tctagcagtt 2340 tgggctgcca attcgcttgg tctggagctg gaggatctgg ccaaggagca gtatcccgag 2400 ctggaggttc ctcttcccaa ctag 2424 <210> 59 <211> 4308 <212> DNA <213> Artificial Sequence <220> <223> YALI0_F12793g <400> 59 atggaacgac tcagccatgg aggcggcgga tcgctcgccg tggtgacgca gtcgccgccg 60 ctcaaccgcc ggctgtcggc agagccccca acctcgcccc agacgtcggc ctcccccaac 120 cgcatccaac cgcaatcgcg tgccgtggcc caagagatca acgccatcca gacgctcaag 180 cggctgtcta tcggtgccgc gctgaccacc gacccggacc tgccggacgc ctaccactcg 240 taccacgtgg cgcaggcgct gggcattgac atggacacgg acgagttcca tgatgcagct 300 gagacgacag acgtggtggg agtgggccgc tcgcggtctg cgggagcggg agccgctact 360 tcgcccacgc ccccggcacg cacactcgcc agacacacgt cactggacac ccccaccaca 420 ccgccccgac ggcgctcctc cgcccgcaag tcgtcgtctt cctcaagctc gtccccagac 480 cgaaactcgc tgctgtgggt cccagcgtct atccatccgg agctggctcc gcaggagtgg 540 aagacgtttg tggaaaacaa ggtggcgggc atggatgagg agacagacac gcgccggctg 600 tcggcgtcgt cgctgtcaag cttgtcacga tctccgtcag ccacatacac ccagactgac 660 gtgggagagg ccaacgtgga acacaagcgc aagtcgggcc tgcaacgctc aaactctcga 720 ctctcaaaac aaatccgcga ccaggaggac tacacagacg gctccgagat tctggagaac 780 cgacgcagat cgtcgctatt gtccacctca acgctcggct ccgtcaccat cgaggagctg 840 tccacgcagc tgtcccagtt gactacacag cagacgaacg cagatccgtt cacactggca 900 cgttcgctgt cggtgtcacg agatggacaa acgtcgcccc aaaagctgca gcgcaaaaag 960 actctggact cgtctccggg agtggactcc atccaggagg aggaagaatc caacacgccg 1020 ctcatgggtc tgcgacgggc caagcggacc aactactcca agccgagtag cattcgtcgt 1080 gctagggccg agtctgccgc tcgagatcgg aacccctttg atgctgattc gccctctcag 1140 actcagactc agacaccatc acaatcacag gttgcggatc ccgccattga tcccgctgat 1200 cctgtttcta caatcgtgga tactgcggac tctcttcctc ctactccccc gcctaaggag 1260 gacaagtatc tgcaggacaa acagctgcac gtggatgttg ctgttgacaa gccgccattg 1320 atggctcccc tcgatggctt ggacaaaccg gtgccttctc ttccgccacc agcagcggga 1380 gaagacttgg acgtgaatgt aaacgtggac aagcccatgc ctccaattcc tggaggagtc 1440 gtgggagcag ttggagcggt ggacgacggg gaggtctcac caaactctcc ggtgcaagcc 1500 gttgagcaga tttctcccaa gcagcagatt ccgcccgaac aggtgtctcc tactcaggtt 1560 tcgcccaaac atgtggtttc caaggagagt gagccagaat tggtgtctca tactgtgaca 1620 gtcactgctc cggctttgga caagccgctt cccgacggaa agcctccgca tgctgctcag 1680 gctccttctc ctgcgcctcc aacagttcct cttcctgctg ctcccgaaga cgacaacggc 1740 ttctcgtttg aagacgacgt taagcagaga agacgaggtg ctcctggcgg tcctcctccg 1800 ggcccgacac ctgttccggt taccagctcc gtccctggcc cttcctctgg ccctggccct 1860 ggccctggcc ccaggccgga tatggctgcc cctcctcctc caggacaggt acagggtcct 1920 cgagatggtc ctatgccccg agatggaccc cgagatggac cccgagatgg accccgagat 1980 ggaccccgag atggaccccg cgatggactc cgagacggtc ctcgggacac tcacggacct 2040 ccccaccacg gataccacgg tggccagccc atgcaccgac aacattcaca tcccccgcat 2100 caacaaccac attctcagca tccccagcaa cagcatcccc agcagcatcc ccggcagcat 2160 ctccagcagc aacaacatca acagcaccag ggcccccctc ctggttataa tggacctcct 2220 ccgaaacaca agcgtcgaga tcgtccacat ggccaaggtc atccacagca gcagggcccc 2280 tatcccgcgc cccagggaca gcagcagggc ccgtaccctg gaccttctgg gcaacacccc 2340 gctcctcacc agggtcacca gggaggtccc taccctcccg cacatcggtc cagaggccat 2400 gcccagggcc aacatgggct ggcgcatgga cctcagaacc atgtccaggg tcacgggccg 2460 aaccacggac cgaaccatgg cccccagggt gggcacgggg gtaatagccc ccagggcgga 2520 cacggtcaca gccggcccca gcagcgtcct ccaggacgag gcaagccggg aactcatggg 2580 ggacctcctg gaccgtacaa gccgggccac aagtcgagcc acagccacag tcccgagctg 2640 aaaagtcatc ccgacttccc ctcgacttca gggtcctccg cggcatccag agacccgtct 2700 catgctcgag atggaagcac ggcgtcggag cccaccccct ctactgttga ctcgccggac 2760 caacacgcgc ctccctcacc gcctcagcct ctgcacaagg agctgagtta caaggagctt 2820 cccatcaaca aggaggcgcc agtggtctac cgtatcacgg acgagcagct gattggcgga 2880 tcatcgttca actcgccatc gatgatgggc cagacgactg acgtgctttc gtcgattcca 2940 ccgtcagagc agctatacgg ccgagggcgc tccaacacgg ctcccagcag cgacgccggc 3000 tacgactcgg atggcgaggt gaccaagacc aagtccaaat cgaagaagag cacgtggggc 3060 tggctactag gcgacaagga caaggacggc aaggacaagg atggaggaaa ggacgccagt 3120 aacgccgagg acgaggtaca cgcgccggcg cagtcttcca acctcatcaa gcgcaaggtc 3180 aagggcaaga aggacgcagc gaccctgatc acgtcgtttt tcaagaaaga caagaagggc 3240 gacgacgaca ttaacacagc aggagaggac aagcgggagg acaagaagga ggtggaccag 3300 agagagcaga gagagcagag ggaacagagg cgagagccag agcttccagt cgacgtgcag 3360 cagcaggcaa caaactctcc tgttcaggcc gtccctgtgc tggctccctc ctcatcgcaa 3420 ccatcgcaat ctggacaacc cggccgtcga ggaacagcac ctgcagctgc ttcgtcggct 3480 cccgaggacc ggtcccgatc tcagtctccg tctcagcgtc gacggatcca aaaggcacgc 3540 aactccaaga gccccaccaa gcgacgacgc gacaagaaca agtccaagtc tgagcacgtg 3600 cgagccgagc tggagcgaca ggacgaccac atggacgtgg atcggaccgt ggtgggccct 3660 ggctcaaagg atatggagga cactgttccg tctgagtcca gtgatggata catggaccct 3720 gcgcagcagc agcagcccat gtactcgccc atcgatccgg ctctgttcga tctgccgtcc 3780 acttacatga agcccaacat gatgacagtg atggactacg agactccgat tttctactcg 3840 ggtcccgcag ttctggtgcc tcccggtgcc ggacgggcca tggtcatgta ctaccatcgg 3900 ttcccgctgc acattgagcg ggccatctac cggctgtctc atttgaagct ggccaaccct 3960 cgtcgcccac tggtgcagca agtgctgctg tccaacttta tgtacgcata tctggatctc 4020 atcaaccagg gctaccagca gcagcagcag cagtacgagg caggggctgg tcaactgggc 4080 gccggagccg gagccggagc tggagctgga gctgatggag ctgcaggaga aaccaccgag 4140 tctgtcgagg ccgacgacta cttctctgaa ggcacctatg accagatggt ggagcagaat 4200 gcgtacgctg ccgttgtgga cgatgacgcc atgggcagtc ctgtaagcac cagcagttcc 4260 agcagtagct ccagcaacga ggagtactgg cacgaggagg gacattag 4308 <210> 60 <211> 1171 <212> DNA <213> Artificial Sequence <220> <223> YALI0_F16577g <400> 60 agtgagtacg aactctggaa tctggtccgt tggaataatt ttttggttgc gattgtgaac 60 actgctgggg ctaccacccg tatagcactg ctctgcactc tcatggcatg tacaagaccc 120 ctctacttga tactgcctca cagcgaacac tagccagcca cattgattgc ttttttttct 180 tgatatttca tggtattggc agcaatcatc atgtttccat ctcattagag ccctgtcatc 240 aagcaactca cctaacacag tgaccctcga atcttcgctt cacctcctca cacctgacca 300 gctttcgtct ctggcccatc tgcaaatttc cgtccccgag ctctgcaaga ctgcccatta 360 tgccgagatt tacggtcacc atttacttcc agatcagaaa tgccccggaa gatactctga 420 gaaggccaga gatgtgattc tgctgaaagt gttggaatcc gaaaactacg acgctactaa 480 ggcaggagac aaactactaa cggtgctgag atggagaaag gactacaagc ctatggatga 540 agacagagac ctaccagtgt tggatggtca cgttactaca gttgctgcta ccaatctgcc 600 tcagaccaca ctctggcatc gctcttcggc aactcacatt cccaactcga tctacattcg 660 atggaaggtt ggattgattg agcaagctat cagtactctt gacttcagct ctcccgacaa 720 ctcttccaag ctcacatgtg tcgaggactt caaccacaac atgtacgatg aagttctatg 780 gaagcagctg agcaccctga gagagttcct cctctatcct ggaattttcg acaagacctt 840 ttttgtcaat gttcctctga gtctcagaat tggactgaga ttcttcaaca agtccaaccc 900 cgtgtacaac cgtcgaaact ccgtcattct cggcaagggc aaggagttga agaagcacct 960 tggtaactgg gtgcctcgag aatatggcgg agaaggacca ggacttgaga tccaaggaag 1020 aaatgtccgc ggttattcag cttatgaaca ccacgctttg cgacatgttc tgaccacttc 1080 tcctacacaa tacagtcatg cgaagggaga cgcataccga gacagctatg gcccaccaga 1140 cgatgacgat gttcctcctt attaccatta g 1171 <210> 61 <211> 3414 <212> DNA <213> Artificial Sequence <220> <223> YALI0_F17468g <400> 61 atgaacgact ttgaagacga ccagaaacat aaggcaagaa ccgagccctc gggccagccg 60 cccaagaaac ggtcaagagt cacattttcg tgtcttattt gccgtaaacg aaagatgaag 120 tgcgaccgca atctgccgtg ctccacctgc aaggccgcca acgccgccca tctctgtcgc 180 tatgacatga accgcagcgc gtcgccccag gacagagaca tgcccatccc cagcgacgag 240 tccatggtgg caccaacgag cgctcctgga ggaggaagcg cggccaactc gggctccaag 300 aagccccgtg gccggcccaa gaagtctgtc gccgagctca ccaagatgca ccagcaactg 360 ctggaacaga acgagctcaa gggcgagacc aaagagctga aggacgtggt caagcaggag 420 ttccagtctc ttcctcccca gcaacagcaa cacatccccc aacagcagca gcagcagcag 480 cagcaccacc aacagcaaca aaccatgcga catgtccccc agcaacagca acagcacatg 540 ccccagcaag cacatcaaca acagcagttc cacgcgctca accaacatca acaccagcac 600 ctgcaacagc agcaccacgc tcagcagcat cacaagccca acggcctaac catcaataca 660 aacgaccctg ctacgcccgt caaccaagcg gtgcgaaaat cgtccgcagc ctcagccact 720 ccgggcagaa cgccccccaa cgaaaactcg cctcgacgtg gatcggccaa gaaacacctg 780 ccgccgatcg gagatacgcc ctcaccgtcc atgtcgtcgc cgtcctccgc attcaaatcg 840 tcggctgaca tgctgcacca ggtggtcacc cggaccgcca cagacaccaa atatattggc 900 atttcgtcga aattctcgct gtgcatgaag cccgcgcgcc agcagttgtt tggacccttc 960 tcgtccatgt ccttcttttc ggaaaacgag cacctgcagc ggttctccaa acaggtgtac 1020 gaggccaaga aacaaaacta catcaaggac aagaacgcac ctgtgcttat gcgccacaaa 1080 aacatattta ccgacttgcc gcaaaagtac cgggtcggcc gaggctcgtt tgtaggggct 1140 ggcagccatt acggaggcac cagtgcgccg ggaagcacaa acagccacgc caatggcggc 1200 ggtgctttca atgcggctgc gtcgcccatg gacaagactc cggcctccac ctcgtcatct 1260 tgctcgacca tggactgtgg agacatttcc aagtctttcc catttgactc gccgaacggt 1320 atctacgagg tactggagct agtccctccc aagcccctga cgtactttct ggtcgacaag 1380 tatatgaact cggtcaaccg gttcttctac acggtcattc ccaaatcctt ctacgagtgt 1440 ctcgacgagt ttttcatcca gaagcagcgc attctgaacc gagagatccc cgccactgtc 1500 aagactgtcg atctgcgaca ggtggcccag attttgctga ttctgcgtct ggctcgaatt 1560 acgctccctt ttgactgggt ggttcccgag tacctggtag gtggcaactc taacagcaca 1620 actggcggtt caaacaatga cggctcctcc gcctcgtctc ctcctgcctc ggcagacacc 1680 tcggacgtgt accttggagc cgggctttct cagattgccg aaaactgcct caaccacatc 1740 ggctatctgc gaaaggccaa cctgcgggta ctccaggtgc tatgtttgct caaaatcacg 1800 gccatgtgcg atcccgacgc cggcgactcg gccgacggct gcgactcgtg caacctcacc 1860 ggtctgatca tccagatctc aatctctatg gggctgcata tggaccccag ccacttctcc 1920 aaggtgtccc caaccattgc tcatctgtgg agaatgctgt ttggtttcat tgtcactctg 1980 gacgcccatc gatccatgga gctggctctg cctccctctc tgcctctgga gtgttcggat 2040 accgacattc tgttcgagcg acactctctg cccgatgacc tgctgtcacc cggcgaggag 2100 cagcacattt accatcgacg aaagcagctg cgatgggcct tcatttctct agatgtggtt 2160 cggggactgc tgcgaacaac agtgcatgtt actcccgatg gaatgggcac ctctccacag 2220 gccatgggca aggagtactt cgatgccatt gagcgcaagc tggaagcatt tgagaacgac 2280 atgaaccctt actacgagga gatcctgtcg gctctacagg cacccgagtc ccgagacagc 2340 gactaccgca ccgtggacgg ccatgctgcc gcccagcggc tgtctctgta catgacacta 2400 gtgcgtctcc gtcttggcta ctacctgtgt gccggcaact ttatggacgc cgccgaggtc 2460 aagaccaagg tggttacgtg tgctcttaag atgtgcgata tcatgcaggc tgctatggag 2520 cgacctcatc tcttctctgg gttcatgtgg tatgtgcatt tcgtcaacct gcgaaacttc 2580 accttctcgt ttggtacttg catttctgca tacctgaacg agatcaacac tcgacgagcc 2640 gagcgcgaca tgcccatcat tactcctgcc gtcaaccgaa agtacaatga catgacgttt 2700 gactattcgc ccgacaagat ccgagatccc aagcggttga ttcaggctgc catcagaact 2760 caccgatggg tccgatctct ttcgcagcgg tactatgttg cctggaagtg tcatgccgtc 2820 attgtcggtc ttcttcgagg tgctcgtctg gaggttctcg cccagtacat tgacgctcaa 2880 gagtacaagc ggctggagaa ggagggccac cgggacaaca cgtttgtggg tccttacgcg 2940 cctcaaatgc gagagcccac aaccatgtcg gttaccaaaa acgactgggg tcgagacatc 3000 acgtcggaga actacgaccg agagcagagc gaacttcgag ctgccgagga gcgtgccaac 3060 cgccaaggtc aaggcccgga gtctatgtct gctcctgcag cgcagtctca ggaccagatt 3120 ggccagggca accagcagca gcagatggag tggcagggtg acaacaacat gtttcagaac 3180 aacggtctga tggagcctcc ctttctagag gacccgatca ttgacgaccc cacctttgat 3240 accctgggct cgactctggg gcttccgtcg cttattgccg aagagtgggc caacattgag 3300 gactcgctct ttgatgatct taactcgtcg cggttctaca tgtccatgga ccagaatttt 3360 aacaacacct ccaccgccac tgacaactat gaggtaactt cgccctccag atag 3414 <210> 62 <211> 2490 <212> DNA <213> Artificial Sequence <220> <223> YALI0_F19030g <400> 62 atgaagttct ctgccactct tctcgctgct gcagtcagcg ttgctgtcgc cgaccctatc 60 ctcggaaagg gacccgaccc caacactttc tggttctccc agccctctac tcccggagcc 120 cccgctcctt ccaaccctcc tgccgctccc gctcccaagc ctaaccccgg cggagttgac 180 cctaacactt tctggttctc gcagtccagc actcctaccc ctgctccccc tgctcccgct 240 cccagcaccc aggctcccgc cccccctgct tcttctcccg ctcctcccgc ttcttcggct 300 gcccctgccc cccagcctga ggagacctgt atcgcccccg ccggcgctca ggagaagcga 360 tctctggccc tcctgtccca ggtcttctgc tccaccaagt gcttcaagct ctctttcggt 420 ctgcagacct gccagggtct ccagtcctgc gagtgtcagt tccttgatgg cttcgcctgg 480 acccagaagt gtctgtcttg tgctcaggct tacccccagt acggcatgca ggagatcgac 540 gacgtcatta agaagtgcca cccccaggcc acctcttctg tcgctccccc tcccgttgtt 600 gtcaccacca ctccccccga tgttaccagc tctcttcccg agtgtgaggt tcctcctccc 660 acttgtaccc ccgagccttc tcaccccgct tctcttgata acatgcttaa caagcgattc 720 aacctgatcg gcaagctcac ctgcaagaag acctgtaaca ccgttgttgc tgacatcaac 780 tcctgtggcc aggatgccaa gtgcatctgt gacaagatca agtccaccca gtctgctgtt 840 gaccagtgca cttcttgtgt ccagcagtac aacatgtggc cttacttcaa cgtcgacaag 900 cagctcctcc agtacgttga ggcttgcaag gctgtcgcca cccccactgc cacccccact 960 cccatcatcc ccgtctgccc taccgagggt gccaccactg agcccgctcc ttcttccacc 1020 cctgcctcct ctacccctgc ctcctctacc cctgcctcct ctacccctgc ctcctctgcc 1080 cccgcttctt ccaagcccga ggagtcttct gccgtcccca cctccgctcc cgttgctccc 1140 accaacgcca ccgagtcttc tgtcgctccc tctaccactg aggctgctac ttcccctgtc 1200 cccaccaacg gaactgtttc ttcctcttcc gttgcttctt ggactaccaa gactctgacc 1260 tctaccgttc cttgccacga gtgcgagcac ggcggcaaca agactgtcat cgtgactgtc 1320 actgagcctt gctctactga ggtccctact accgccgatg ttcctactac ctccgaggtt 1380 cctaccacct ccgttgaggc tgtccctacc tccgaggctc ccgtccagaa caccacccag 1440 actgtcactg aggagtgtga gacttgcaag cactcttcta ctgctgctcc ttctctcacc 1500 accaagacca ccgtcattga gggtaagact gtcaccatca ctgagccctg ctccactgag 1560 gcccccaccg aggcccccga ggctacttct gcccccgtta ccaccaaggt caccgttgtt 1620 gagggcaaga ctgtcactct cactgaggag tgtgaggagt gcaagcacca ctctaccgag 1680 gcccccaagc ccaagcctac ctccgagtac cccgagggag tttctctcac taccaaggtc 1740 actgtgatcg agggagttac caagactgtt accgttcctt gcgatactac cactgaggct 1800 cccgtccccg agacttctgc ccctgctccc aagcccgagt cttctgctcc ccccgctcct 1860 gcccctgctc ctgagtcccc cgccggcaag cccgagccta cccccgctcc tgctccctct 1920 tctgctcctg ctcccgctcc caagcccgag tcttctgctc ctgctcccgc tcccaagccc 1980 gagtcttctg ctcctgctcc cgctcccaag cccgagtctt ctgctcctgc tcccgctccc 2040 aagcccgagt cttctgctcc tgctcccgct cccaagcccg agacttctgc tcctgctccc 2100 gctcccaagc ccgaggattc tgcccctgct cctgctccca agcctgagga gtctgcccct 2160 gctcccgctc ccaagcccga gtcttctgct cctcccgctc ctgctcctgc tcctgagtct 2220 cccgccgtca agcccgagtc tacccccgcc cccgcccctg cccccgctcc tgctcctgct 2280 cctgagtctt ctgctcctcc cgcccctgcc cccaagcccg aggcttctgc tcctcccgct 2340 ggcaagcccg aggttcccca ggctcagcct tccacccccg agcaggctcc ccaggctccc 2400 tcttccggcc ctgcccaggc taacggcgct gcctccaacg gtatctccgc cgctatcctt 2460 gccgctgctg gtatccttgc tcttttctaa 2490 <210> 63 <211> 3339 <212> DNA <213> Artificial Sequence <220> <223> YALI0_F23287g <400> 63 atgctccgag acgtgagcaa gtcgactctc aacattatgg aagcaggtgc cacggacacg 60 tgtgggcgcg acggcgacgg actccacgac gctcgctcgc aggtcgagcc acaacacgtg 120 gactacgcca tcaacgacga ctcggtgata cccgactcgg agtgcgagca ggaggtgctc 180 aacacggact acgtcggcgg cgtgagcaag tcccacagac agggccatca agggggtgtg 240 gtggaagaca ccacgttcag cgatcttctg gaaatgaaca tcaactttgc cccgggatcc 300 atggaggtgg gcgagttgcg gacggaggaa gtcacgcccg aggtggagga gtgttttcgg 360 ggttttgagc gccaaatgat gagccacgtg agccacagca acgtgagcca cagcaacgtg 420 agccacagca acgtgagcca caactccatg ctcggaagct atccgatcca gagccactgt 480 ggatccatag gccagagcct agggtatttc gccagtgagg aactgactga ggtggaaaaa 540 gggaccattc atgatgagcg ggagcaggaa cagaagcaag agcaggaaca gaagcaagag 600 caggaacagg aggagcagat ggaggagggt aatgaagaac agagcaacga acagcccgtg 660 actgaacagt cggagtctgt tagtggaagc gaggagggtc aggaggttga ggcaagtgaa 720 gctggtgttc tagctgtcat tgaagatgaa gttggagaga atggcgagcc tggtggagcg 780 ggcgattctt ctcatgccat tgaaatcaca tctactagtc cttccaagat cgagattgct 840 attgaagatg aagatgaagg tgaagatgaa gatgaagatg aagatgaaga tgaaggtgag 900 gctgaagtag acgaagaggc agaagaagtc gaagacgagc tagaagccga gtcagagact 960 acgccaaagg tttctgcgtc cgaaaacgag ttctcacaca catcgcactt tgaaactcca 1020 gcagctgaga caccaaaaca cgacgaggag gttatcgcag ttgaagccga tcaagacgtg 1080 tccatgatga acgtgagcat ggaccgaacc aaccacgatg tgagcatggc agaaacaact 1140 gcagtggtca acaccactag tggagatact actgtcacag acgaagcttc tacagctcta 1200 acagcaccca ctacacacac acatgcagaa gacactgcat cgcaggctac tgtttcgcgt 1260 gagtcgtcag cccaagttgg agcaactctc ggaacaagtg ctaagcccga agaaacagaa 1320 ggagatacaa cagtcactgt gacggcctct gctactccag agaacacagg tatggacgag 1380 ggtcctcctg tcctatctct cttggacgta gatggtgatg tccatatgga gatgcaagag 1440 gccaatggtg aggttaatga gcgcgataca catggggagg atacacatga ggaggaggag 1500 gagatgacag gggaggacgg cgacgagctg gcagatggcg aagactacga tatggcaggc 1560 gaagcagacg agcaaccaca gaaacagact attacagaga ctgctccaac agtgtcaccg 1620 gttgcatctt tcccagcact tcccatgaac acagctagac ttactcggga acacaagacg 1680 attcccggtg aggtaacacc agaagccaaa ttgattccag gtgaagttcc tggtgagaag 1740 cgtctcattc ctggtgagaa gcctgttgcc ggttctacga agaagcccat cccaggagag 1800 attctatctg ccaaacgacc tcttcttgac gagactcccg tgtctcgatc agctcccact 1860 gttgccacat ctaaactcat tcctggagag gttcctcgaa acacatcatc atcatcatcc 1920 tctgcatcga agattcttca gccgacacca cgagtggcat tgtcaatgaa gcctccggag 1980 tcgctggttt cgagtgttcg agcatcgtct cagcctcctc gtcagctgac tcccattccc 2040 aatctttcgc attccgacgc tcttccagaa gaccctaccg accaggctcg acaggctctg 2100 gctcgaaaaa gacctctccc caagcgtccc cagggccgta agatctctca cgagaccact 2160 ctggagctgc agaacttcga gttgtcttgc aagggtcttt ctgatcacta tcggcttctg 2220 gacaaggttg gcgagggtac tttctcctca gtctaccttg ccgaagacat tcattacgac 2280 ctcaagcgtg aatttgctgc tcggaagaag cagaaccagt cgtccagcca ctggaagtct 2340 ccccctttga actccaagaa gcggattcga ttgctggagc gttcaaacac agatatccat 2400 cggggtccac ttgtggccat caagcgcatc tacgtgacgt cttcgcccaa acgaattctt 2460 aacgaaatca agattcttga cgcgctctca ggctacgaca atatatcgcc actgttaaag 2520 gtgatgcgcc atgaggacca aattctcgcg gtactgccgt actttgagca tcaggatttt 2580 cggtcgctgt acaaaaccgg ctccaagacg gacatccgta tttatattac ccagctgtgc 2640 caggccctca agtttgtgca ttccaaggac atcatccacc gagacatcaa gcctaccaac 2700 ttcctctacg accgtcaccg acggtacggg gtgctggtgg actttggact ggcagaagtg 2760 cagtcatttt cgacgtccaa tccctgtgtg tgtgtttctc aggaaaacca gaaacgtatt 2820 ttgggctcgg cgtgggagct caagcccaag ggctcatacg agaagaacga cgtgcggtct 2880 ggccggcgaa gcaaccgagc aggtactcga ggctttcgag cccccgaggt gctgttcaag 2940 tgcacctgcc agacgaccca gatcgacatg tggtccgtgg gcgtgatctt gctgtcattc 3000 ctgtgcaaga ctctgccttt cttcatgtcg cacgacgatg ctgatgctct ggtggagcta 3060 tgcagcattt ttggccgcca ggctatgcac cagacgggtc ttctacatgg tgcttttctt 3120 gagctccaga tccccaaact gcgaagtaca ggcctgccgt ttgagcgggt tctgttacag 3180 gctggatgcc ccgaggagtg ggctcgagat ccggtggtga ttgattttct cagcaacacc 3240 atgaagctgg atcaccacca tcggttttcc gccgagaagg ctttggccca tgagttttgc 3300 cagcgtgcct atgaggagga tgattacgta gacgtataa 3339

Claims (20)

  1. YALI0_F17468g; 또는
    YALI0_F17468g, 및 YALI0_A15642g, YALI0_A15796g, YALI0_C11165g, YALI0_C16247g, YALI0_D24849g, YALI0_D27016g, YALI0_E14388g1, YALI0_E23969g 및 YALI0_F04906g로 이루어진 군으로부터 선택된 하나 이상;
    의 유전자에서 하기 돌연변이를 포함하고,
    D-자일로스(D-xylose)와 D-자일룰로스(D-xylulose)를 상호 변환하는 효소를 코딩하는 유전자 및 D-자일룰로스(D-xylulose)로부터 D-자일룰로스-5-인산염(D-xylulose-5-phosphate)을 생산하는 효소를 코딩하는 유전자를 포함하며,
    자일로스(xylose)를 유일 탄소원으로 이용할 수 있는, 형질전환된 적응진화 효모 균주:
    YALI0_A15642g 유전자에서 147G>A 돌연변이;
    YALI0_A15796g 유전자에서 567_568insACA 및 576T>G 돌연변이;
    YALI0_C11165g 유전자에서 1523T>C 돌연변이;
    YALI0_C16247g 유전자에서 T1412A, G1441A, A1513G, A1534G, G1535T, T1544A 및 C1847T 돌연변이;
    YALI0_D24849g 유전자에서 103G>A 돌연변이;
    YALI0_D27016g 유전자에서 446T>C 돌연변이;
    YALI0_E14388g1 유전자에서 2525C>G, 2531T>G, 2534A>G 및 2558T>C 돌연변이;
    YALI0_E23969g 유전자에서 480_481insTCCTCTACCCCCGAG, 526_527insCCTCCACCACCGAGC 및 497A>C 돌연변이;
    YALIO_F04906g 유전자에서 2702T>A; 또는
    YALI0_F17468g 유전자에서 482_483insGCACCA 돌연변이.
  2. 제1항에 있어서, 상기 균주는 YALI0_A00891g, YALI0_A00935g, YALI0_A02002g, YALI0_A02497g, YALI0_A07997g, YALI0_A13849g, YALI0_A16863g, YALI0_A17578g, YALI0_A17776g, YALI0_A17853g, YALI0_A19646g, YALI0_B00748g, YALI0_B08800g, YALI0_C06424g, YALI0_C07172g, YALI0_C08437g, YALI0_C09031g, YALI0_C09614g, YALI0_C13728g, YALI0_C14476g, YALI0_C15532g, YALI0_C16148g, YALI0_D15752g, YALI0_D17820g, YALI0_D18381g, YALI0_D19822g, YALI0_D20064g, YALI0_D20526g, YALI0_D20790g, YALI0_D24563g, YALI0_D25014g, YALI0_D25058g, YALI0_D26257g, YALI0_D26510g, YALI0_D26620g, YALI0_E07832g, YALI0_E08008g, YALI0_E11363g, YALI0_E13596g, YALI0_E16731g, YALI0_E18073g, YALI0_E18216g, YALI0_E20449g, YALIO_E21109g, YALI0_F12221g, YALI0_F12793g, YALI0_F16577g, YALI0_F19030g 및 YALI0_F23287g로 이루어진 군으로부터 선택된 하나 이상의 유전자에서 하기 돌연변이를 추가로 포함하는, 형질전환된 적응진화 효모 균주:
    YALI0_A00891g 유전자에서 11_16delACGGCC 돌연변이;
    YALI0_A00935g 유전자에서 202_203insGCTC 돌연변이;
    YALI0_A02002g 유전자에서 1255_1256insGAGGTCAAGGTC 돌연변이;
    YALI0_A02497g 유전자에서 132_137dupCAACTC 및 331_332insCCCACT 돌연변이;
    YALI0_A07997g 유전자에서 2846_2847insGGAGCAGGA 및 2869_2870insAGGAGGAGG 돌연변이;
    YALI0_A13849g 유전자에서 165_166insCAAA 돌연변이;
    YALI0_A16863g 유전자에서 888_889insGAGCTGAGATGAC 돌연변이;
    YALI0_A17578g 유전자에서 432_433insGCGGAGCCG 돌연변이;
    YALI0_A17776g 유전자에서 959_960insACAGCAGAT 돌연변이;
    YALI0_A17853g 유전자에서 1940_1942delAGG, 1990_1991insAGGAGGAGGCTAAGAAGA 및 2817_2818insTCTGAG 돌연변이;
    YALI0_A19646g 유전자에서 1462_1463insGGG 돌연변이;
    YALI0_B00748g 유전자에서 525_526insCCCGAC 돌연변이;
    YALI0_B08800g 유전자에서 152_153delGT 돌연변이;
    YALI0_C06424g 유전자에서 1370_1373delACTT 돌연변이;
    YALI0_C07172g 유전자에서 308_309insGCAGCGACA 돌연변이;
    YALI0_C08437g 유전자에서 1592_1593insGACAGTCAGCAC 돌연변이;
    YALI0_C09031g 유전자에서 1789_1790insCTCCCGAGTCCTCTGCTGAGCCTACCAGCGAAGAGACTTCTTCCG 돌연변이;
    YALI0_C09614g 유전자에서 1620_1622delACA 돌연변이;
    YALI0_C13728g 유전자에서 99_100insAAAAAGTGGTCGAAAAAGTGGCCA 및 129_130insTGGCCGAAAAAGTGGCCAAAA 돌연변이;
    YALI0_C14476g 유전자에서 778_779insTGC 돌연변이;
    YALI0_C15532g 유전자에서 1611_1616delCAGCTT 돌연변이;
    YALI0_C16148g 유전자에서 961_969delAGCAGCAGT 돌연변이;
    YALI0_D15752g 유전자에서 1038_1039insCAG 돌연변이;
    YALI0_D17820g 유전자에서 619_620insCCCACCCGCAAACCC 돌연변이;
    YALI0_D18381g 유전자에서 114_115insCCTCTCACCAACTCA 돌연변이;
    YALI0_D19822g 유전자에서 310_315delAAAGAG 돌연변이;
    YALI0_D20064g 유전자에서 67_68insGGGGGGGG 돌연변이;
    YALI0_D20526g 유전자에서 354_355insTCCACCGGA 돌연변이;
    YALI0_D20790g 유전자에서 37_38insCACGTGAAAGTAGCCGAA 돌연변이;
    YALI0_D24563g 유전자에서 84_85insGCT 돌연변이;
    YALI0_D25014g 유전자에서 484_485insCCGCTAGCGCCAACTCTGGCTCGGAGC 돌연변이;
    YALI0_D25058g 유전자에서 592_594dupAAG 돌연변이;
    YALI0_D26257g 유전자에서 1478_1480delAGA, 돌연변이;
    YALI0_D26510g 유전자에서 1287_1288insAAG 돌연변이;
    YALI0_D26620g 유전자에서 926_927insCGATGAGGACGA 돌연변이;
    YALI0_E07832g 유전자에서 499_500insCCAAGCCCCCCGCTTCCAAGCCCACCGCTT 돌연변이;
    YALI0_E08008g 유전자에서 794_795insCTCTTCCTCTTCCTCTTCCTCTTCCTCTTC 돌연변이;
    YALI0_E11363g 유전자에서 74_77delCACA 돌연변이;
    YALI0_E13596g 유전자에서 1053_1054insCAACAACAACAGCAACAA 돌연변이;
    YALI0_E16731g 유전자에서 1622_1623insTGAGGAGGAAGAGTAGGATGAGGAGTA 돌연변이;
    YALI0_E18073g 유전자에서 266_267insCCCCACGCAGCAGTCTTG 돌연변이;
    YALI0_E18216g 유전자에서 930_938delACAACAGCA 돌연변이;
    YALI0_E20449g 유전자에서 899_900insAAACGC 돌연변이;
    YALI0_E21109g 유전자에서 228_229insGCCCCGCCT 돌연변이;
    YALI0_F12221g 유전자에서 1825_1827delAAG 돌연변이;
    YALI0_F12793g 유전자에서 1855_1860delTCTTCT 돌연변이;
    YALI0_F16577g 유전자에서 807_808insCCTCCT 돌연변이;
    YALI0_F19030g 유전자에서 1344_1345insCCTACTACCGCCGATGTT, 2065T>A, 2098A>G 및 2099C>A 돌연변이; 또는
    YALI0_F23287g 유전자에서 1919_1920insCTC 돌연변이.
  3. 제1항에 있어서,
    상기 효모 균주는 야로위아 리폴리티카(Yarrowia lipolytica) 균주인, 형질전환된 적응진화 효모 균주.
  4. 제1항에 있어서,
    상기 D-자일로스와 D-자일룰로스를 상호 변환하는 효소는 자일로스 이성화효소(xylose isomerase)인, 형질전환된 적응진화 효모 균주.
  5. 제1항에 있어서,
    상기 D-자일룰로스로부터 D-자일룰로스-5-인산염을 생산하는 효소는 자일룰로스 인산화효소(xylulokinase)인, 형질전환된 적응진화 효모 균주.
  6. 제1항에 있어서,
    상기 D-자일로스와 D-자일룰로스를 상호 변환하는 효소를 코딩하는 유전자는 xylA(xylose isomerase) 유전자인, 형질전환된 적응진화 효모 균주.
  7. 제1항에 있어서,
    상기 D-자일로스와 D-자일룰로스를 상호 변환하는 효소를 코딩하는 유전자는 서열번호 1의 염기서열로 이루어지는, 형질전환된 적응진화 효모 균주.
  8. 제1항에 있어서,
    상기 D-자일룰로스로부터 D-자일룰로스-5-인산염을 생산하는 효소를 코딩하는 유전자는 XK(xylulokinase) 유전자인, 형질전환된 적응진화 효모 균주.
  9. 제1항에 있어서,
    상기 D-자일룰로스로부터 D-자일룰로스-5-인산염을 생산하는 효소를 코딩하는 유전자는 서열번호 2의 염기서열로 이루어지는, 형질전환된 적응진화 효모 균주.
  10. 제1항에 있어서,
    상기 균주는 제 1 벡터 및 제 2 벡터로 이루어진 군으로부터 선택된 하나 이상의 벡터로 형질전환된 것으로,
    상기 제 1 벡터는 D-자일로스와 D-자일룰로스를 상호 변환하는 효소를 코딩하는 유전자를 포함하고,
    상기 제 2 벡터는 D-자일룰로스로부터 D-자일룰로스-5-인산염을 생산하는 효소를 코딩하는 유전자를 포함하는, 형질전환된 적응진화 효모 균주.
  11. 제10항에 있어서,
    상기 제 1 벡터는 UAS1B 인헨서, TEF(Translational elongation factor) 프로모터, 및 D-자일로스와 D-자일룰로스를 상호 변환하는 효소를 코딩하는 유전자를 포함하는, 형질전환된 적응진화 효모 균주.
  12. 제11항에 있어서,
    상기 제 1 벡터는 서열번호 3의 염기서열로 이루어지는, 형질전환된 적응진화 효모 균주.
  13. 제10항에 있어서,
    상기 제 2 벡터는 UAS1B 인헨서, TEF(Translational elongation factor) 프로모터, 및 D-자일룰로스로부터 D-자일룰로스-5-인산염을 생산하는 효소를 코딩하는 유전자를 포함하는, 형질전환된 적응진화 효모 균주.
  14. 제13항에 있어서,
    상기 제 2 벡터는 서열번호 4의 염기서열로 이루어지는, 형질전환된 적응진화 효모 균주.
  15. 제1항에 있어서,
    상기 균주는 자일로스가 유일 탄소원으로 존재하는 배지에서 3회 이상 계대배양된, 형질전환된 적응진화 효모 균주.
  16. 제1항에 있어서,
    상기 유전자 돌연변이를 포함하는 효모 균주는 기탁번호가 KCTC13615BP인, 형질전환된 적응진화 효모 균주.
  17. 제1항에 있어서,
    상기 형질전환된 적응진화 효모 균주는 기탁번호가 KCTC13616BP인, 형질전환된 적응진화 효모 균주.
  18. 제1항 내지 제17항 중 어느 한 항의 형질전환된 적응진화 효모 균주 제조방법으로,
    야생형 효모 균주를 유일 탄소원으로 자일로스를 포함하는 배지에서 3회 이상 계대배양하는 단계; 및
    상기 계대배양된 균주가 D-자일로스(D-xylose)와 D-자일룰로스(D-xylulose)를 상호 변환하는 효소를 코딩하는 유전자 및 D-자일룰로스로(D-xylulose)부터 D-자일룰로스-5-인산염(D-xylulose-5-phosphate)을 생산하는 효소를 코딩하는 유전자를 포함하도록 형질전환하는 단계를 포함하는, 균주 제조방법.
  19. 제1항 내지 제17항 중 어느 한 항의 형질전환된 적응진화 효모 균주를 배양하는 단계를 포함하는, 지질 생산 방법.
  20. 제1항 내지 제17항 중 어느 한 항의 형질전환된 적응진화 효모 균주를 탄소원으로 자일로스를 포함하는 배지에서 배양하여 지질을 생산하는 단계; 및
    상기 생산된 지질을 트랜스에스테르화하여 바이오디젤을 수득하는 단계;를 포함하는, 바이오디젤 생산 방법.
KR1020180117860A 2018-10-02 2018-10-02 자일로스 이용능을 갖는 효모 균주 KR102108190B1 (ko)

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