DK2483295T3 - Polypeptider med cellulolytisk forbedrende aktivitet og polynukleotider, der koder for dem - Google Patents

Polypeptider med cellulolytisk forbedrende aktivitet og polynukleotider, der koder for dem Download PDF

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DK2483295T3
DK2483295T3 DK10763938.7T DK10763938T DK2483295T3 DK 2483295 T3 DK2483295 T3 DK 2483295T3 DK 10763938 T DK10763938 T DK 10763938T DK 2483295 T3 DK2483295 T3 DK 2483295T3
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polypeptide
vol
cellulosic material
acid
fermentation
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DK10763938.7T
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Lan Tang
Ye Liu
Junxin Duan
Zhang Yu
Christian Jorgensen
Randall Kramer
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Novozymes Inc
Novozymes As
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    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
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    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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Claims (13)

1. Isoleret polypeptid med cellulolytisk forbedrende aktivitet, der er udvalgt fra gruppen, som består af: (a) et polypeptid, som omfatter en aminosyresekvens, der udviser mindst 80% identitet med det modne polypeptid ifølge SEQ ID NO: 2; og (b) et polypeptid, der kodes for af et polynukleotid, som omfatter en nukleotidsekvens, der udviser mindst 80% identitet med det modne polypeptids kodningssekvens ifølge SEQ ID NO: 1.
2. Polypeptid ifølge krav 1, som omfatter eller består af aminosyresekvensen ifølge SEQ ID NO: 2 eller det modne polypeptid ifølge SEQ ID NO: 2; eller et fragment deraf med cellulolytisk forbedrende aktivitet.
3. Polypeptid ifølge krav 1, hvilket polypeptid kodes for af polynukleotidet indeholdt i plasmid pGEM-T-GH61 D23Y4, hvilket plasmid er indeholdt i E. coli DSM 22882.
4. Isoleret polynukleotid, som omfatter en nukleotidsekvens, der koder for polypeptidet ifølge et hvilket som helst af kravene 1-3.
5. Fremgangsmåde til fremstilling af polypeptidet ifølge et hvilket som helst af kravene 1-3, hvilken fremgangsmåde omfatter: (a) dyrkning af en celle, som i sin vildtypeform producerer polypeptidet, under betingelser, som er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
6. Fremgangsmåde til fremstilling af et polypeptid med cellulolytisk forbedrende aktivitet, hvilken fremgangsmåde omfatter: (a) dyrkning af en rekombinant værtscelle, som omfatter polynukleotidet ifølge krav 4, under betingelser, som er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
7. Detergentsammensætning, som omfatter polypeptidet ifølge et hvilket som helst af kravene 1-3.
8. Fremgangsmåde til nedbrydning eller omdannelse af et celluloseholdigt materiale, hvilken fremgangsmåde omfatter: behandling af det celluloseholdige materiale med en enzymsammensætning i nærværelse af polypeptidet med cellulolytisk forbedrende aktivitet ifølge et hvilket som helst af kravene 1-3. 1
9. Fremgangsmåde ifølge krav 8, som endvidere omfatter indvinding af det nedbrudte celluloseholdige materiale.
10. Fremgangsmåde til fremstilling af et fermenteringsprodukt, hvilken fremgangsmåde omfatter: (a) forsukring af et celluloseholdigt materiale med en enzymsammensætning i nærværelse af polypeptidet med cellulolytisk forbedrende aktivitet ifølge et hvilket som helst af kravene 1-3; (b) fermentering af det forsukrede celluloseholdige materiale med en eller flere (adskillige) fermenterende mikroorganismer for at fremstille fermenteringsproduktet; og (c) indvinding af fermenteringsproduktet fra fermenteringen.
11. Fremgangsmåde til fermentering af et celluloseholdigt materiale, hvilken fremgangsmåde omfatter: fermentering af det celluloseholdige materiale med en eller flere (adskillige) fermenterende mikroorganismer, hvor det celluloseholdige materiale er forsukret med en enzymsammensætning i nærværelse af et polypeptid med cellulolytisk forbedrende aktivitet ifølge et hvilket som helst af kravene 1-3.
12. Fremgangsmåde ifølge krav 11, hvor fermenteringen af det celluloseholdige materiale frembringer et fermenteringsprodukt.
13. Fremgangsmåde ifølge krav 12, som endvidere omfatter indvinding af fermenteringsproduktet fra fermenteringen. 2
DK10763938.7T 2009-09-29 2010-09-29 Polypeptider med cellulolytisk forbedrende aktivitet og polynukleotider, der koder for dem DK2483295T3 (da)

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PCT/US2010/050700 WO2011041397A1 (en) 2009-09-29 2010-09-29 Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same

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US (6) US8148103B2 (da)
EP (1) EP2483295B1 (da)
CN (1) CN102695719B (da)
CA (1) CA2775358A1 (da)
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ES (1) ES2560805T3 (da)
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