DK2195421T3 - Polypeptider med acetylxylanesteraseaktivitet og polynukleotider, der koder for dem - Google Patents

Polypeptider med acetylxylanesteraseaktivitet og polynukleotider, der koder for dem Download PDF

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DK2195421T3
DK2195421T3 DK08833334.9T DK08833334T DK2195421T3 DK 2195421 T3 DK2195421 T3 DK 2195421T3 DK 08833334 T DK08833334 T DK 08833334T DK 2195421 T3 DK2195421 T3 DK 2195421T3
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polypeptide
sequence
cell
seq
polynucleotide
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DK08833334.9T
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De Leon Alfredo Lopez
Hanshu Ding
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Novozymes As
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)

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Claims (14)

1. Isoleret polypeptid med acetylxylanesteraseaktivitet, der er udvalgt fra gruppen, som består af: (a) et polypeptid, som omfatter en aminosyresekvens med mindst 80%, mere fortrinsvis mindst 85%, endnu mere fortrinsvis mindst 90% og mest fortrinsvis mindst 95%, mindst 96%, mindst 97%, mindst 98% eller mindst 99% identitet med det modne polypeptid ifølge SEQ ID NO: 2; (b) et polypeptid, der kodes for af et polynukleotid, som omfatter en nukleotidsekvens, hvilken nukleotidsekvens fortrinsvis har mindst 85%, mere fortrinsvis mindst 90%, endnu mere fortrinsvis mindst 95% og mest fortrinsvis mindst 97% identitet med det modne polypeptids kodningssekvens ifølge SEQ ID NO: 1.
2. Polypeptid ifølge krav 1, som omfatter eller består af aminosyresekvensen ifølge SEQ ID NO: 2; eller et fragment deraf, som har acetylxylanesteraseaktivitet og omfatter mindst 380 aminosyrerester.
3. Polypeptid ifølge krav 1, der kodes for af et polynukleotid, som omfatter eller består af nukleotidsekvensen ifølge SEQ ID NO: 1; eller en undersekvens deraf, der koder for et fragment med acetylxylanesteraseaktivitet.
4. Polypeptid ifølge krav 1, hvilket polypeptid kodes for af polynukleotidet indeholdt i plasmid pTterAxe2A, hvilket plasmid er indeholdt i den deponerede bakteriestamme E. coli NRRL B-50067.
5. Isoleret polynukleotid, som omfatter en nukleotidsekvens, der koder for polypeptidet ifølge et hvilket som helst af kravene 1 -4.
6. Nukleinsyrekonstruktion, som omfatter polynukleotidet ifølge krav 5 operabelt bundet til en eller flere kontrolsekvenser, der styrer produktionen af polypeptidet i en ekspressionsvært.
7. Rekombinant værtscelle, som omfatter nukleinsyrekonstruktionen ifølge krav 6.
8. Fremgangsmåde til fremstilling af polypeptidet ifølge et hvilket som helst af kravene 1 -4, hvilken fremgangsmåde omfatter: (a) dyrkning af en celle, som i sin vildtypeform producerer polypeptidet, under betingelser, som er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
9. Fremgangsmåde til fremstilling af polypeptidet ifølge et hvilket som helst af kravene 1-4, hvilken fremgangsmåde omfatter: (a) dyrkning af en værtscelle, som omfatter en nukleinsyrekonstruktion, hvilken nukleinsyrekonstruktion omfatter en nukleotidsekvens, der koder for polypeptidet, under betingelser, som er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
10. Fremgangsmåde til fremstilling af polypeptidet ifølge et hvilket som helst af kravene 1-4, hvilken fremgangsmåde omfatter: (a) dyrkning af en transgen plante eller en plantecelle, som omfatter et polynukleotid, hvilket polynukleotid koder for polypeptidet, under betingelser, som er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
11. Transgen plante, plantedel eller plantecelle transformeret med et polynukleotid, hvilket polynukleotid koder for polypeptidet ifølge et hvilket som helst af kravene 1 -4.
12. Fremgangsmåde til nedbrydning af en acetyleret xylan, hvilken fremgangsmåde omfatter behandling af et materiale, som omfatter en acetyleret xylan, med en sammensætning, der omfatter en effektiv mængde af polypeptidet med acetylxylanesteraseaktivitet ifølge et hvilket som helst af kravene 1-4.
13. Fremgangsmåde ifølge krav 12, hvor sammensætningen endvidere omfatter et eller flere xylannedbrydende enzymer.
14. Fremgangsmåde ifølge krav 13, hvor det xylannedbrydende enzym eller de xylannedbrydende enzymer er valgt fra gruppen bestående af en xylanase, en arabinofuranosidase, en xylosidase og en glucuronidase.
DK08833334.9T 2007-09-28 2008-09-26 Polypeptider med acetylxylanesteraseaktivitet og polynukleotider, der koder for dem DK2195421T3 (da)

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US97615207P 2007-09-28 2007-09-28
PCT/US2008/077823 WO2009042846A1 (en) 2007-09-28 2008-09-26 Polypeptides having acetylxylan esterase activity and polynucleotides encoding same

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US (1) US8034996B2 (da)
EP (1) EP2195421B1 (da)
CN (1) CN101903518B (da)
BR (1) BRPI0817471A2 (da)
DK (1) DK2195421T3 (da)
WO (1) WO2009042846A1 (da)

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