DK2450435T3 - Maltotriosyl-transferase, fremgangsmåde til fremstilling deraf, og anvendelse deraf - Google Patents

Maltotriosyl-transferase, fremgangsmåde til fremstilling deraf, og anvendelse deraf Download PDF

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DK2450435T3
DK2450435T3 DK10793893.8T DK10793893T DK2450435T3 DK 2450435 T3 DK2450435 T3 DK 2450435T3 DK 10793893 T DK10793893 T DK 10793893T DK 2450435 T3 DK2450435 T3 DK 2450435T3
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maltotriosyl transferase
enzyme
dna
gly
asn
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DK10793893.8T
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Masamichi Okada
Miho Nagaya
Shotaro Yamaguchi
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Amano Enzyme Inc
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06—Enzymes
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

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  • Health & Medical Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Cereal-Derived Products (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (12)

1. Maltotriosyl-transferase, der virker på polysaccharider og oligosaccharider med a-1,4 glucosidbindinger til at overføre maltotriose-enheder til saccharider, kendetegnet ved at virke på maltotetraose som substrat for at give et forhold mellem maltoheptaose-produktionsraten og maltotriose-produktionsraten på 9:1 til 10:0 ved en hvilken som helst substratkoncentration i området fra 0,67 til 70 % (w/v); og omfattende aminosyresekvensen fremsat i SEQ ID NO: 8 eller en aminosyresekvens med mindst 70 % identitet med aminosyresekvensen fremsat i SEQ ID NO: 8.
2. Enzymprodukt omfattende maltotriosyl-transferasen ifølge krav 1 som en aktiv ingrediens.
3. Mikroorganisme med en evne til at producere maltotriosyl-transferase ifølge krav 1, hvor mikroorganismen er Geobacillus sp. APC9669 (accessionsnummer NITE BP-770).
4. Maltotriosyl-transferase-gen omfattende et hvilket som helst af DNA valgt fra gruppen bestående af de følgende (a) til (d): (a) DNA, der koder for aminosyresekvensen fremsat i SEQ ID NO: 7 eller 8; (b) DNA, der omfatter sekvensen fremsat i SEQ ID NO: 6; (c) DNA, der hybridiserer med den komplementære sekvens af sekvensen fremsat i SEQ ID NO: 6 under stringente forhold og der koder for en maltotriosyl-transferase, kendetegnet ved at virke på maltotetraose som substrat for at give et forhold mellem maltoheptaose-produktionsraten og maltotriose-produktionsraten på 9:1 til 10:0 ved en hvilken som helst substratkoncentration i området fra 0,67 til 70 % (w/v); (d) DNA, der er degenereret fra DNA-sekvensen af sekvensen fremsat i SEQ ID NO: 6.
5. Rekombinant vektor omfattende maltotriosyl-transferase-genet ifølge krav 4, der fortrinsvis er en ekspressionsvektor.
6. Ikke-human transformant, der indeholder den rekombinante vektor ifølge krav 5.
7. Transformanten ifølge krav 6, hvilken er en bakteriecelle, en gærcelle eller en svampecelle.
8. Fremgangsmåde til fremstilling af en maltotriosyl-transferase, omfattende de følgende trin (1) og (2), eller trinnene (i) og (ii): (1) at dyrke mikroorganismen ifølge krav 3 og (2) at indsamle maltotriosyl-transferasen fra kulturopløsningen og/eller bakteriecellerne efter dyrkning; eller (i) at dyrke transformanten ifølge krav 6 eller 7 under forhold egnede til produktionen af proteinet kodet for af maltotriosyl-transferase-genet; og (ii) at indsamle det derved producerede protein.
9. Anvendelse af enzymet ifølge krav 1 eller enzymproduktet ifølge krav 2 til fremstilling og behandling afen fødevare, der indeholderet polysaccharid eller oligosaccharid med a-1,4 glucosidbindinger.
10. Fremgangsmåde til fremstilling af fødevare eller fødevareingrediens omfattende at udsætte en fødevare, der indeholder et polysaccharid eller oligosaccharid med α-1,4 glucosidbindinger, for maltotriosyl-transferasen ifølge krav 1 eller enzymproduktet ifølge krav 2.
11. Fremgangsmåden ifølge krav 10, hvilken udføres i højtemperatur-miljø.
12. Anvendelse af enzymet ifølge krav 1, eller enzym produktet ifølge krav 2 til at forbedre fødevare eller fødevareingrediens i sin funktion.
DK10793893.8T 2009-07-01 2010-03-20 Maltotriosyl-transferase, fremgangsmåde til fremstilling deraf, og anvendelse deraf DK2450435T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2009156569 2009-07-01
PCT/JP2010/054894 WO2011001722A1 (ja) 2009-07-01 2010-03-20 マルトトリオシル転移酵素及びその製造方法並びに用途

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DK2450435T3 true DK2450435T3 (da) 2017-09-25

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US (3) US8546111B2 (da)
EP (1) EP2450435B1 (da)
JP (2) JP5762958B2 (da)
KR (1) KR20120103545A (da)
CN (1) CN102510900B (da)
BR (1) BRPI1014606A2 (da)
CA (1) CA2766018A1 (da)
DK (1) DK2450435T3 (da)
WO (1) WO2011001722A1 (da)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI1014606A2 (pt) * 2009-07-01 2015-08-25 Amano Enzyme Inc Maltotriosil transferase, processo para produção e uso da mesma
CN105950686A (zh) * 2011-02-04 2016-09-21 天野酶制品株式会社 麦芽三糖基转移酶的新用途
US20160177356A1 (en) * 2011-02-04 2016-06-23 Amano Enzyme Inc. Novel use of maltotriosyl transferase
US20160264928A1 (en) * 2015-03-11 2016-09-15 Magnegas Corporation System, Method, and Apparatus for Sterilization with Additional Nutrients for Microbiological Inoculation
CN106709899B (zh) * 2015-07-15 2020-06-02 华为终端有限公司 双摄像头相对位置计算方法、装置和设备
CN105647827A (zh) * 2015-12-09 2016-06-08 天津北洋百川生物技术有限公司 麦芽三糖形成酶的高产菌株及其筛选和培养方法
US11034900B2 (en) 2017-08-08 2021-06-15 Magnegas Ip, Llc System, method, and apparatus for gasification of a solid or liquid
JPWO2019066031A1 (ja) * 2017-09-29 2020-10-22 サントリーホールディングス株式会社 アクアポリン３発現促進用組成物及びその使用
CN107648255B (zh) * 2017-10-11 2020-06-09 西南大学 一种含麦芽七糖的有机抗菌剂及其制备方法和应用
CN108220362B (zh) * 2018-03-07 2021-07-16 江南大学 一种利用环糊精水解酶制备特定聚合度麦芽低聚糖的方法
WO2020069396A1 (en) * 2018-09-27 2020-04-02 Archer Daniels Midland Company Dietary fiber production using a glycosyl-transferase
EP3875589A4 (en) * 2018-10-31 2022-10-19 Amano Enzyme Inc. MALTOTRIOSE-PRODUCING AMYLASE
JP7354541B2 (ja) * 2019-01-15 2023-10-03 味の素株式会社 穀類食品の製造方法および品質低下抑制方法、ならびに穀類食品の品質低下抑制剤
JP7319823B2 (ja) * 2019-05-16 2023-08-02 オリエンタル酵母工業株式会社 パン類用品質保持剤、パン類の製造方法およびパン類の品質保持方法
JP7245715B2 (ja) * 2019-05-28 2023-03-24 オリエンタル酵母工業株式会社 パン類用品質向上剤、パン類の品質向上方法およびパン類の製造方法
KR102170296B1 (ko) * 2019-06-17 2020-10-27 대한민국(농촌진흥청장) 고중합 이소말토올리고당 제조용 당전이효소 및 상기 당전이효소를 이용하여 고중합 이소말토올리고당을 제조하는 방법
KR102320952B1 (ko) * 2019-10-28 2021-11-04 대한민국(농촌진흥청장) 고중합 이소말토올리고당 제조용 당전이효소를 이용한 이소말툴로오스 유래 올리고당 제조방법
TW202203782A (zh) * 2020-04-16 2022-02-01 日商味之素股份有限公司 抑制血糖值上升之米飯
JP7468875B2 (ja) * 2020-07-01 2024-04-16 株式会社Mizkan Holdings 米飯、その製造方法、炊飯用調味液、米飯改良剤及びその使用方法
WO2024095954A1 (ja) * 2022-10-31 2024-05-10 天野エンザイム株式会社 米飯の製造方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512463A (en) 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
JP3743525B2 (ja) 1994-03-02 2006-02-08 タカラバイオ株式会社 部位特異的変異導入方法
JP3456756B2 (ja) * 1994-05-30 2003-10-14 天野エンザイム株式会社 パン類の品質改良組成物およびそれを用いたパン類の製造法
JP3526326B2 (ja) 1994-09-05 2004-05-10 タカラバイオ株式会社 部位特異的変異導入方法
JPH08140685A (ja) 1994-11-22 1996-06-04 Takara Shuzo Co Ltd 部位特異的変異導入方法
WO1998002535A1 (fr) 1996-07-11 1998-01-22 Takara Shuzo Co., Ltd. Procede pour effectuer une mutagenese dirigee
JPH10262661A (ja) * 1997-03-25 1998-10-06 Cci Corp 新規なα−アミラーゼおよびその単離方法
JP2001294601A (ja) 2000-04-11 2001-10-23 Akita Prefecture 高度分岐澱粉と該高度分岐澱粉の製造方法
TWI312369B (en) 2000-08-01 2009-07-21 Hayashibara Biochem Lab A-isomaltosylglucosaccharide synthase,process for producing the same and use thereof
US20080190845A1 (en) * 2005-09-02 2008-08-14 Novozymes North America, Inc. Methods for Enhancing the Dewaterability of Sludge with Alpha-Amylase Treatment
JP4862759B2 (ja) * 2006-06-30 2012-01-25 味の素株式会社 澱粉含有食品の製造方法及び澱粉含有食品改質用の酵素製剤
WO2008092919A1 (en) * 2007-02-01 2008-08-07 Novozymes A/S Alpha-amylase and its use
JP5173593B2 (ja) 2007-05-28 2013-04-03 花王株式会社 換気扇又はレンジフード用防汚フィルタ
BRPI1014606A2 (pt) * 2009-07-01 2015-08-25 Amano Enzyme Inc Maltotriosil transferase, processo para produção e uso da mesma

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EP2450435A4 (en) 2012-12-26
CN102510900A (zh) 2012-06-20
JP2015156861A (ja) 2015-09-03
KR20120103545A (ko) 2012-09-19
EP2450435B1 (en) 2017-08-16
CN102510900B (zh) 2015-05-13
JPWO2011001722A1 (ja) 2012-12-13
US20120100253A1 (en) 2012-04-26
EP2450435A1 (en) 2012-05-09
BRPI1014606A2 (pt) 2015-08-25
US20140004226A1 (en) 2014-01-02
US8765434B2 (en) 2014-07-01
US20140011257A1 (en) 2014-01-09
CA2766018A1 (en) 2011-01-06
JP6115921B2 (ja) 2017-04-19
WO2011001722A1 (ja) 2011-01-06
US8546111B2 (en) 2013-10-01
JP5762958B2 (ja) 2015-08-12

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