DK2209893T3 - Anvendelse af aptamerer i proteomik - Google Patents

Anvendelse af aptamerer i proteomik Download PDF

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Publication number
DK2209893T3
DK2209893T3 DK08837175.2T DK08837175T DK2209893T3 DK 2209893 T3 DK2209893 T3 DK 2209893T3 DK 08837175 T DK08837175 T DK 08837175T DK 2209893 T3 DK2209893 T3 DK 2209893T3
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Prior art keywords
aptamer
aptamers
molecule
sample
protein
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DK08837175.2T
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English (en)
Inventor
Koen Kas
Sven Agnes Jan Eyckerman
Clive Gavin Brown
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Pronota Nv
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Plant Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Claims (28)

1. En fremgangsmåde til måling af mængden af mindst et molekyle i en biologisk prøve, hvilken fremgangsmåde omfatter, at man: a) kombinerer prøven med én eller flere aptamerer, og tillader ét eller flere molekyler i prøven at binde til aptameren eller aptamererne; b) adskiller bundne molekyler fra ikke-bundne molekyler, og c) kvantificerer det/de molekyle(r), der er bundet til aptameren eller til hver aptamer, hvor kvantificering af det/de bundne molekyle(r) udføres ved sekventering af mindst en del af aptameren, eller ved sekventering af hver aptamer.
2. Fremgangsmåden ifølge krav 1, hvor det mindst ene molekyle er et protein.
3. Fremgangsmåden ifølge krav 1 eller krav 2, hvor identiteten af molekylet er kendt.
4. Fremgangsmåden ifølge krav 1 eller krav 2, hvor identiteten af molekylet er ukendt.
5. Fremgangsmåden ifølge krav 4, hvor fremgangsmåden yderligere omfatter en bestemmelse af identiteten af molekylet.
6. Fremgangsmåden ifølge et hvilket som helst af kravene 1 til 5, hvor sekvensen af aptameren, eller af hver aptamer, er kendt.
7. Fremgangsmåden ifølge et hvilket som helst af kravene 1 til 6, hvor hver aptamer sekvens bærer et unikt mærke.
8. Fremgangsmåden ifølge krav 7, hvor mærket er sekvensen af aptameren.
9. Fremgangsmåden ifølge krav 7 eller krav 8, hvori mærket udgør en del af sekvensen af aptameren.
10. Fremgangsmåden ifølge et hvilket som helst af kravene 1 til 9, hvor sekventering udføres i et enkeltmolekyle array eller et klonalt enkeltmolekyle array.
11. Fremgangsmåden ifølge et hvilket som helst af kravene 1 til 10, hvor fremgangsmåden yderligere omfatter at fjerne aptamer(er) bundet til molekylet, eller til hvert molekyle, og array-præsentation af aptamer(er) på en overflade.
12. Fremgangsmåden ifølge krav 11, hvor fremgangsmåden yderligere omfatter at amplificere de array-præsenterede aptamerer.
13. Fremgangsmåden ifølge et hvilket som helst af kravene 1 til 12, hvor én eller flere aptamerer omfatter forskellige sekvenser, der binder til det samme molekyle.
14. Fremgangsmåden ifølge et hvilket som helst af kravene 1 til 12, hvor én eller flere aptamerer omfatter paneler af aptamersekvenser, idet hvert panel binder til et andet molekyle.
15. Fremgangsmåden ifølge et hvilket som helst af kravene 1 til 14, hvor aptamererne er afledt af polynukleotider.
16. Fremgangsmåden ifølge krav 15, hvor aptamererne er polynukleotider og har mellem ca. 30 og ca. 60 baser.
17. Fremgangsmåden ifølge krav 16, hvor aptamererne har ca. 40 baser.
18. Fremgangsmåden ifølge et hvilket som helst af kravene 1 til 17, hvor den biologiske prøve er en legemsvæske.
19. Fremgangsmåden ifølge krav 18, hvor legemsvæsken er blod eller afledt af blod.
20. Fremgangsmåden ifølge krav 19, hvor legemsvæsken er serum eller plasma.
21. Fremgangsmåden ifølge et hvilket som helst af kravene 1 til 20, hvor fremgangsmåden yderligere omfatter: d) at kombinere en anden biologisk prøve med den bundne aptamer fraktion opnået i c); e) at adskille bundne molekyler fra ikke-bundne molekyler; f) at kvantificere det/de molekyle(er), der er bundet til aptamerer, og g) at sammenligne mængder opnået i c) med de mængder, der opnås i f)> hvor kvantificering af aptamerer bundet til et eller flere molekyler i den anden prøve udføres ved sekventering af mindst en del af hver aptamer.
22. Fremgangsmåden ifølge et hvilket som helst af kravene 1 til 21, hvor fremgangsmåden yderligere omfatter sammenligning af mængden af aptameren, eller af mængden af hver aptamer, med en kontrol- eller baseline mængde.
23. Fremgangsmåden ifølge krav 21 eller krav 22, hvor den anden prøve er opnået fra et individ kendt for at være i en sygdomstilstand.
24. Fremgangsmåden ifølge krav 21 eller krav 22, hvor den anden prøve er opnået fra et individ, der er kendt for at være i en rask tilstand.
25. Fremgangsmåden ifølge krav 21 eller krav 22, hvor den anden prøve er opnået fra et individ efter behandling med et lægemiddel.
26. Anvendelse af en fremgangsmåde ifølge et hvilket som helst af kravene 1 til 25 til identifikation af en eller flere biomarkører.
27. Anvendelse af en fremgangsmåde ifølge et hvilket som helst af kravene 1 til 25 til validering af en eller flere biomarkører.
28. Anvendelse af en fremgangsmåde ifølge et hvilket som helst af kravene 1 til 25 som en diagnostisk fremgangsmåde.
DK08837175.2T 2007-10-12 2008-10-10 Anvendelse af aptamerer i proteomik DK2209893T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07020049 2007-10-12
PCT/GB2008/003447 WO2009047526A1 (en) 2007-10-12 2008-10-10 Use of aptamers in proteomics

Publications (1)

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DK2209893T3 true DK2209893T3 (da) 2014-02-17

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DK08837175.2T DK2209893T3 (da) 2007-10-12 2008-10-10 Anvendelse af aptamerer i proteomik

Country Status (8)

Country Link
US (6) US20100298152A1 (da)
EP (1) EP2209893B1 (da)
JP (1) JP5766948B2 (da)
CN (2) CN101896605A (da)
DK (1) DK2209893T3 (da)
ES (1) ES2448426T3 (da)
PT (1) PT2209893E (da)
WO (1) WO2009047526A1 (da)

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Also Published As

Publication number Publication date
CN101896605A (zh) 2010-11-24
US11624083B2 (en) 2023-04-11
US20180030505A1 (en) 2018-02-01
US20150087536A1 (en) 2015-03-26
EP2209893B1 (en) 2013-11-20
CN103134938B (zh) 2017-03-01
US9758811B2 (en) 2017-09-12
US20220002780A1 (en) 2022-01-06
CN103134938A (zh) 2013-06-05
ES2448426T3 (es) 2014-03-13
US20220325315A1 (en) 2022-10-13
US20100298152A1 (en) 2010-11-25
PT2209893E (pt) 2014-02-26
JP5766948B2 (ja) 2015-08-19
EP2209893A1 (en) 2010-07-28
US20190382823A1 (en) 2019-12-19
US10995360B2 (en) 2021-05-04
JP2011500012A (ja) 2011-01-06
US10316349B2 (en) 2019-06-11
WO2009047526A1 (en) 2009-04-16

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