DK2205749T3 - Modificerede zinkfingerproteiner, som er målrettet mod 5-enolpyruvylshikimat-3-phosphat-syntasegener - Google Patents

Modificerede zinkfingerproteiner, som er målrettet mod 5-enolpyruvylshikimat-3-phosphat-syntasegener Download PDF

Info

Publication number
DK2205749T3
DK2205749T3 DK08833882.7T DK08833882T DK2205749T3 DK 2205749 T3 DK2205749 T3 DK 2205749T3 DK 08833882 T DK08833882 T DK 08833882T DK 2205749 T3 DK2205749 T3 DK 2205749T3
Authority
DK
Denmark
Prior art keywords
sequence
epsps
sequences
gene
cleavage
Prior art date
Application number
DK08833882.7T
Other languages
English (en)
Inventor
Manju Gupta
Asha M Palta
Stephen Novak
Fyodor Urnov
Sunita Gopalan
Original Assignee
Dow Agrosciences Llc
Sangamo Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40243886&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK2205749(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dow Agrosciences Llc, Sangamo Biosciences Inc filed Critical Dow Agrosciences Llc
Application granted granted Critical
Publication of DK2205749T3 publication Critical patent/DK2205749T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Claims (20)

1
1. Zinkfingerprotein (ZFP), som binder sig til en genomisk EPSPS-målregion af interesse, hvilket ZFP omfatter et eller flere zinkfingerbindingsdomæner, hvor 5 ZFP'et omfatter de genkendelseshelixregioner, der er vist i en enkelt række i tabel A.
2. ZFP ifølge krav 1, hvor ZFP'et er et fusionsprotein, som omfatter et eller flere funktionelle domæner valgt fra gruppen bestående af en 10 transskriptionsrepressor, en endonuklease, en methyltransferase, en histondeacetylase, en transskriptionsaktivator og en histonacetyltransferase.
3. ZFP ifølge krav 2, hvor ZFP'et er en zinkfingernuklease (ZFN), som spalter den genomiske EPSPS-målregion af interesse, hvor ZFN'en omfatter et eller 15 flere modificerede zinkfingerbindingsdomæner og et nukleasespaltningsdomæne, og hvor ZFN'en endvidere eventuelt spalter en eller flere paraloge eller ortologe EPSPS-gensekvenser, der omfatter en nukleotidsekvens valgt fra gruppen bestående af SEQ ID NO: 10-14 eller en hvilken som helst sekvens, som udviser mindst ca. 80-100% sekvensidentitet 20 dermed.
4. Polynukleotid, som koder for et eller flere zinkfingerproteiner (ZFP'er) ifølge et hvilket som helst af kravene 1-3.
5. Planteværtscelle, som omfatter et eller flere polynukleotider ifølge krav 4, hvor cellen er forbigående eller stabilt transficeret med polynukleotidet eller polynukleotiderne.
6. Fremgangsmåde til spaltning af et eller flere EPSPS-gener i en plantecelle, 30 hvilken fremgangsmåde omfatter: indføring af et eller flere polynukleotider ifølge krav 4 i plantecellerne og ekspression af ZFN'en eller ZFN'erne i cellen, hvor ZFN'erne spalter et eller fbra PPQDQ.nonar 2
7. Donorvektor, som omfatter en første og en anden DNA-sekvens; hvor den første sekvens er homolog med en tredje sekvens, og den anden sekvens er homolog med en fjerde sekvens; og hvor den tredje og den fjerde sekvens er 5 kromosomale EPSPS-DNA-sekvenser, og endvidere hvor de nære kanter af den tredje og den fjerde sekvens er sammenhængende eller adskilt af mindst 1 nukleotidpar, og eventuelt hvor vektoren endvidere omfatter en femte sekvens, hvor den femte sekvens: (a) er indskudt mellem den første og den anden sekvens og 10 (b) er en eksogen nukleinsyresekvens, og hvor de homologe sekvenser udviser 80-100% sekvensidentitet med hinanden, og hvor de kromosomale EPSPS-DNA-sekvenser tilhører et EPSPS-gen, som omfatter en nukleotidsekvens valgt fra gruppen bestående af SEQ ID NO: 10-14 15 eller en sekvens, som udviser mindst ca. 80-100% sekvensidentitet dermed.
8. Vektor ifølge krav 7, hvor den femte sekvens omfatter sekvenser, som koder for en selekterbar markør valgt fra gruppen bestående af grøntfluorescerende protein (GFP), β-glucuronidase (GUS), phosphinothricin-N- 20 acetyltransferase (PAT, BAR), neomycinphosphotransferase, hygromycinphosphotransferase, β-lactamase, catecholdioxygenase, a-amylase, tyrosinase, β-galactosidase, luciferase, aequorin, EPSP-syntase, nitrilase, acetolactatsyntase (ALS), dihydrofolatreduktase (DHFR), dalapondehalogenase og anthranilatsyntase, eller hvor den femte sekvens omfatter en sekvens, som 25 koder for et protein, der ikke er en selekterbar markør; en eller flere transskriptionsregulatorsekvenser; en eller flere proteinmålsøgningssekvenser, der øger eller mindsker proteintransport; et lille interfererende RNA, der er målrettet mod et gen, som skal represseres; og et mikro-RNA, der er målrettet mod et gen, som skal represseres. 30
9. Vektor ifølge et hvilket som helst af kravene 7 eller 8, hvor den femte sekvens omfatter en sekvens, som koder for en muteret kromosomal EPSPS- οδΙ/\/δπο Har mr%c^r nhntae tnlaranra nv/ar ir\r h^rhir^iH^t nl\/nhnoot 3
10. Fremgangsmåde til indføring af en eksogen nukleinsyresekvens i en plantecelles genom, hvilken fremgangsmåde omfatter følgende trin: ekspression af en eller flere zinkfingernukleaser (ZFN'er) ud fra et polynukleotid 5 ifølge krav 4 i cellen i nærværelse af en donorvektor ifølge et hvilket som helst af kravene 7-9; hvor ZFN'en eller ZFN'erne spalter et EPSPS-gen i kromosomalt DNA inden for 3 kilobasepar fra enten den tredje eller den fjerde sekvens; således at spaltning af det kromosomale DNA i trin (b) stimulerer til 10 inkorporering af donorvektoren i genomet ved hjælp af homolog rekombination.
11. Fremgangsmåde til ekspression af produktet af et eksogent nukleinsyresekvensmolekyle i en plantecelle, hvilken fremgangsmåde omfatter følgende trin: 15 indføring af det eksogene sekvensmolekyle i henhold til fremgangsmåden ifølge krav 10, således at produktet af det eksogene sekvensmolekyle udtrykkes i plantecellen.
12. Transgen plantecelle eller transgen plante, som er opnået ved 20 fremgangsmåden ifølge krav 10 eller 11.
13. Fremgangsmåde til stimulering til intramolekylær homolog rekombination i en plantecelles genom, hvilken fremgangsmåde omfatter følgende trin: indføring af en ZFN ifølge krav 3 eller et polynukleotid ifølge krav 4 i 25 plantecellen i nærværelse af et DNA-segment, som omfatter et EPSPS-gen og en første sekvens, der er homolog med en anden sekvens i plantecellen, således at ZFN'en spalter DNA-segmentet og stimulerer til intramolekylær homolog rekombination, hvor homolog rekombination resulterer i en addition, deletion eller substitution af sekvenser i det endogene DNA-segment. 30
14. Fremgangsmåde ifølge krav 13, hvor de deleterede sekvenser er valgt fra gruppen bestående af sekvenser, som koder for et EPSPS-gen i sin helhed 4 eller en del deraf, og sekvenser, som koder for en selekterbar markør i sin helhed eller en del deraf.
15. Fremgangsmåde ifølge krav 13 eller 14, hvor det deleterede DNA 5 substitueres med en eksogen sekvens, hvilken fremgangsmåde endvidere omfatter: indføring af et polynukleotid i cellen, hvor polynukleotidet omfatter: (a) en fjerde og en femte sekvens, hvor den tjerde sekvens er homolog med ikke-deleterede sekvenser i nærheden af den første sekvens, og den femte 10 sekvens er homolog med ikke-deleterede sekvenser i nærheden af den anden sekvens; og (b) den eksogene sekvens.
16. Fremgangsmåde ifølge krav 15, hvor den eksogene sekvens, som indføres 15 ved homolog rekombination, omfatter en muteret kromosomal EPSPS-sekvens, der øger en plantes tolerance over for herbicidet glyphosat.
17. Fremgangsmåde til deletion af en EPSPS-gensekvens fra en plantecelles genom, hvilken fremgangsmåde omfatter: 20 ekspression af en første og en anden zinkfingernuklease (ZFN) ifølge krav 3 eller et polynukleotid ifølge krav 4 i cellen, hvor den første ZFN spalter ved et første spaltningssted i et EPSPS-gen, og den anden ZFN spalter ved et andet spaltningssted i EPSPS-genet, hvor EPSPS-gensekvensen er placeret mellem det første spaltningssted og det andet spaltningssted, hvor spaltning af det 25 første og det andet spaltningssted resulterer i deletion af EPSPS-gensekvensen, hvor EPSPS-gensekvensen deleteres ved ikke-homolog endeforbindelse af det første og det andet spaltningssted.
18. Fremgangsmåde ifølge krav 17, hvor plantecellen er en transgen 30 plantecelle, som omfatter en eksogen EPSPS-sekvens.
19. Fremgangsmåde til modulering af ekspressionen af et EPSPS-gen, hvilken framrionnomårla AmfaHar· 5 ekspression af et ZFP i cellen, hvilket ZFP er som defineret i krav 1 eller 2, hvor ZFP'et binder sig til et målsted i EPSPS-genet, og hvor ekspressionen af EPSPS-genet derved moduleres.
20. Fremgangsmåde ifølge krav 19, hvor ZFP'et øger eller mindsker transskriptionen af EPSPS-genet, eller hvor ZFP'et øger eller mindsker en plantes tolerance over for herbicidet glyphosat.
DK08833882.7T 2007-09-27 2008-09-25 Modificerede zinkfingerproteiner, som er målrettet mod 5-enolpyruvylshikimat-3-phosphat-syntasegener DK2205749T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US99555707P 2007-09-27 2007-09-27
PCT/US2008/011089 WO2009042164A1 (en) 2007-09-27 2008-09-25 Engineered zinc finger proteins targeting 5-enolpyruvyl shikimate-3-phosphate synthase genes

Publications (1)

Publication Number Publication Date
DK2205749T3 true DK2205749T3 (da) 2016-08-22

Family

ID=40243886

Family Applications (1)

Application Number Title Priority Date Filing Date
DK08833882.7T DK2205749T3 (da) 2007-09-27 2008-09-25 Modificerede zinkfingerproteiner, som er målrettet mod 5-enolpyruvylshikimat-3-phosphat-syntasegener

Country Status (16)

Country Link
US (3) US8399218B2 (da)
EP (2) EP3072973B1 (da)
JP (3) JP5702144B2 (da)
CN (1) CN101878307B (da)
AR (1) AR068559A1 (da)
AU (1) AU2008305568B2 (da)
BR (1) BRPI0817560B1 (da)
CA (2) CA2937438C (da)
DK (1) DK2205749T3 (da)
ES (1) ES2585157T3 (da)
HR (1) HRP20161004T1 (da)
HU (1) HUE029916T2 (da)
PT (1) PT2205749T (da)
SI (1) SI2205749T1 (da)
TW (1) TWI424062B (da)
WO (1) WO2009042164A1 (da)

Families Citing this family (121)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003251286B2 (en) 2002-01-23 2007-08-16 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
CN102625655B (zh) * 2008-12-04 2016-07-06 桑格摩生物科学股份有限公司 使用锌指核酸酶在大鼠中进行基因组编辑
DK2370575T3 (da) * 2008-12-17 2018-02-05 Dow Agrosciences Llc Målrettet integration i zp15-locuset
JP2012529287A (ja) * 2009-06-11 2012-11-22 トゥールゲン インコーポレイション 部位−特異的ヌクレアーゼを用いた標的ゲノムの再配列
EP2491127B1 (en) * 2009-10-22 2017-09-20 Dow AgroSciences LLC Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis
US9457077B2 (en) 2009-11-18 2016-10-04 Katherine Rose Kovarik Method and system for targeting the microbiome to promote health and treat allergic and inflammatory diseases
US9585920B2 (en) 2011-02-04 2017-03-07 Katherine Rose Kovarik Method and system for treating cancer cachexia
MX336846B (es) 2010-01-22 2016-02-03 Sangamo Biosciences Inc Alteracion genomica dirigida.
UA113493C2 (xx) * 2010-01-22 2017-02-10 Спосіб видалення ділянки днк в рослині
CA2794037A1 (en) * 2010-03-22 2011-09-29 Philip Morris Products S.A. Modifying enzyme activity in plants
EP3156062A1 (en) 2010-05-17 2017-04-19 Sangamo BioSciences, Inc. Novel dna-binding proteins and uses thereof
BR112013016631A2 (pt) * 2010-12-29 2016-10-04 Dow Agrosciences Llc análise de dados de sequências de dna
US20120252122A1 (en) * 2011-01-05 2012-10-04 Sangamo Biosciences, Inc. Methods and compositions for increasing production of induced pluripotent stem cells (ipscs)
CN102174649B (zh) * 2011-01-18 2013-06-12 陕西师范大学 快速检测锌指核酸酶介导基因定点整合的方法
US10835560B2 (en) 2013-12-20 2020-11-17 Joseph E. Kovarik Reducing the likelihood of skin cancer in an individual human being
US10687975B2 (en) 2011-02-04 2020-06-23 Joseph E. Kovarik Method and system to facilitate the growth of desired bacteria in a human's mouth
US10512661B2 (en) 2011-02-04 2019-12-24 Joseph E. Kovarik Method and system for reducing the likelihood of developing liver cancer in an individual diagnosed with non-alcoholic fatty liver disease
US9730967B2 (en) 2011-02-04 2017-08-15 Katherine Rose Kovarik Method and system for treating cancer cachexia
US10085938B2 (en) 2011-02-04 2018-10-02 Joseph E. Kovarik Method and system for preventing sore throat in humans
US10010568B2 (en) 2011-02-04 2018-07-03 Katherine Rose Kovarik Method and system for reducing the likelihood of a spirochetes infection in a human being
US11951140B2 (en) 2011-02-04 2024-04-09 Seed Health, Inc. Modulation of an individual's gut microbiome to address osteoporosis and bone disease
US11523934B2 (en) 2011-02-04 2022-12-13 Seed Health, Inc. Method and system to facilitate the growth of desired bacteria in a human's mouth
US11273187B2 (en) 2015-11-30 2022-03-15 Joseph E. Kovarik Method and system for reducing the likelihood of developing depression in an individual
US10548761B2 (en) 2011-02-04 2020-02-04 Joseph E. Kovarik Method and system for reducing the likelihood of colorectal cancer in a human being
US11419903B2 (en) 2015-11-30 2022-08-23 Seed Health, Inc. Method and system for reducing the likelihood of osteoporosis
US10245288B2 (en) 2011-02-04 2019-04-02 Joseph E. Kovarik Method and system for reducing the likelihood of developing NASH in an individual diagnosed with non-alcoholic fatty liver disease
US10842834B2 (en) 2016-01-06 2020-11-24 Joseph E. Kovarik Method and system for reducing the likelihood of developing liver cancer in an individual diagnosed with non-alcoholic fatty liver disease
US11844720B2 (en) 2011-02-04 2023-12-19 Seed Health, Inc. Method and system to reduce the likelihood of dental caries and halitosis
US10940169B2 (en) 2015-11-30 2021-03-09 Joseph E. Kovarik Method for reducing the likelihood of developing cancer in an individual human being
US11951139B2 (en) 2015-11-30 2024-04-09 Seed Health, Inc. Method and system for reducing the likelihood of osteoporosis
US10583033B2 (en) 2011-02-04 2020-03-10 Katherine Rose Kovarik Method and system for reducing the likelihood of a porphyromonas gingivalis infection in a human being
US10111913B2 (en) 2011-02-04 2018-10-30 Joseph E. Kovarik Method of reducing the likelihood of skin cancer in an individual human being
US11191665B2 (en) 2011-02-04 2021-12-07 Joseph E. Kovarik Method and system for reducing the likelihood of a porphyromonas gingivalis infection in a human being
US11998479B2 (en) 2011-02-04 2024-06-04 Seed Health, Inc. Method and system for addressing adverse effects on the oral microbiome and restoring gingival health caused by sodium lauryl sulphate exposure
US10314865B2 (en) 2011-02-04 2019-06-11 Katherine Rose Kovarik Method and system for treating cancer and other age-related diseases by extending the healthspan of a human
US11357722B2 (en) 2011-02-04 2022-06-14 Seed Health, Inc. Method and system for preventing sore throat in humans
US10086018B2 (en) 2011-02-04 2018-10-02 Joseph E. Kovarik Method and system for reducing the likelihood of colorectal cancer in a human being
US9987224B2 (en) 2011-02-04 2018-06-05 Joseph E. Kovarik Method and system for preventing migraine headaches, cluster headaches and dizziness
EA201391373A1 (ru) 2011-03-23 2014-07-30 Пайонир Хай-Бред Интернэшнл, Инк. Способы получения сложного локуса трансгенных признаков
WO2012149470A1 (en) 2011-04-27 2012-11-01 Amyris, Inc. Methods for genomic modification
WO2013050611A1 (en) 2011-10-07 2013-04-11 Basf Plant Science Company Gmbh Method of producing plants having increased resistance to pathogens
WO2013050593A1 (en) 2011-10-07 2013-04-11 Basf Plant Science Company Gmbh Method of producing plants having increased resistance to pathogens
WO2013050318A1 (en) 2011-10-07 2013-04-11 Basf Plant Science Company Gmbh Method of producing plants having increased resistance to pathogens
WO2013053686A1 (en) 2011-10-10 2013-04-18 Basf Plant Science Company Gmbh Method of producing plants having increased resistance to pathogens
WO2013053711A1 (en) 2011-10-10 2013-04-18 Basf Plant Science Company Gmbh Method of producing plants having increased resistance to pathogens
EP2780460B1 (en) 2011-11-16 2018-07-11 Sangamo Therapeutics, Inc. Modified dna-binding proteins and uses thereof
KR20140109909A (ko) * 2011-12-30 2014-09-16 다우 아그로사이언시즈 엘엘씨 합성 양방향성 ｓｃｂｖ 식물 프로모터를 위한 방법 및 구축물
CA2855902C (en) * 2011-12-30 2022-06-21 Dow Agrosciences Llc Construct and method for synthetic bidirectional plant promoter ubi1
EP2612918A1 (en) 2012-01-06 2013-07-10 BASF Plant Science Company GmbH In planta recombination
AU2013256240B2 (en) 2012-05-02 2018-09-20 Corteva Agriscience Llc Targeted modification of malate dehydrogenase
CN104540951B (zh) * 2012-06-07 2017-07-04 美国陶氏益农公司 使用芸苔属双向组成型启动子表达转基因的构建体和方法
UA119135C2 (uk) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
JP6775953B2 (ja) 2012-09-07 2020-10-28 ダウ アグロサイエンシィズ エルエルシー Ｆａｄ３性能座および標的化切断を誘導可能である対応する標的部位特異的結合タンパク質
UA118090C2 (uk) 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
SG11201503059XA (en) * 2012-10-23 2015-06-29 Toolgen Inc Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
US20150291967A1 (en) * 2012-10-31 2015-10-15 Luc Mathis Coupling herbicide resistance with targeted insertion of transgenes in plants
JP2016500268A (ja) * 2012-12-13 2016-01-12 ダウ アグロサイエンシィズ エルエルシー トウモロコシにおける特定の遺伝子座に対する精密な遺伝子標的化
US10227610B2 (en) 2013-02-25 2019-03-12 Sangamo Therapeutics, Inc. Methods and compositions for enhancing nuclease-mediated gene disruption
KR102192599B1 (ko) 2013-04-05 2020-12-18 다우 아그로사이언시즈 엘엘씨 식물의 게놈 내의 외인성 서열의 통합을 위한 방법 및 조성물
EP2984101B1 (en) * 2013-04-05 2020-08-19 Qiagen Sciences, LLC Kits and methods for isolating protein from biological and environmental samples
CN116083487A (zh) * 2013-05-15 2023-05-09 桑格摩生物治疗股份有限公司 用于治疗遗传病状的方法和组合物
MX2016002306A (es) * 2013-08-22 2016-07-08 Du Pont Promotor u6 de polimerasa iii de soja y metodos de uso.
EP3988654A1 (en) 2013-08-28 2022-04-27 Sangamo Therapeutics, Inc. Compositions for linking dna-binding domains and cleavage domains
EP4279496A3 (en) 2013-09-04 2024-03-13 Corteva Agriscience LLC Rapid targeting analysis in crops for determining donor insertion
SG10201801782PA (en) * 2013-09-04 2018-04-27 Csir Site-specific nuclease single-cell assay targeting gene regulatory elements to silence gene expression
EP4074330A1 (en) * 2013-09-05 2022-10-19 Massachusetts Institute of Technology Tuning microbial populations with programmable nucleases
EP3057432B1 (en) 2013-10-17 2018-11-21 Sangamo Therapeutics, Inc. Delivery methods and compositions for nuclease-mediated genome engineering in hematopoietic stem cells
ES2881473T3 (es) 2013-10-17 2021-11-29 Sangamo Therapeutics Inc Métodos de suministro y composiciones para la modificación por ingeniería genética del genoma mediada por nucleasas
EP3066109A4 (en) 2013-11-04 2017-11-29 Dow AgroSciences LLC Optimal soybean loci
NZ746567A (en) 2013-11-04 2019-09-27 Dow Agrosciences Llc Optimal soybean loci
CA2928855A1 (en) 2013-11-04 2015-05-07 Dow Agrosciences Llc Optimal maize loci
KR102269769B1 (ko) 2013-11-04 2021-06-28 코르테바 애그리사이언스 엘엘씨 최적 메이즈 유전자좌
US11998574B2 (en) 2013-12-20 2024-06-04 Seed Health, Inc. Method and system for modulating an individual's skin microbiome
US11969445B2 (en) 2013-12-20 2024-04-30 Seed Health, Inc. Probiotic composition and method for controlling excess weight, obesity, NAFLD and NASH
US11672835B2 (en) 2013-12-20 2023-06-13 Seed Health, Inc. Method for treating individuals having cancer and who are receiving cancer immunotherapy
US11642382B2 (en) 2013-12-20 2023-05-09 Seed Health, Inc. Method for treating an individual suffering from bladder cancer
US11839632B2 (en) 2013-12-20 2023-12-12 Seed Health, Inc. Topical application of CRISPR-modified bacteria to treat acne vulgaris
US11026982B2 (en) 2015-11-30 2021-06-08 Joseph E. Kovarik Method for reducing the likelihood of developing bladder or colorectal cancer in an individual human being
US11529379B2 (en) 2013-12-20 2022-12-20 Seed Health, Inc. Method and system for reducing the likelihood of developing colorectal cancer in an individual human being
US11213552B2 (en) 2015-11-30 2022-01-04 Joseph E. Kovarik Method for treating an individual suffering from a chronic infectious disease and cancer
US11833177B2 (en) 2013-12-20 2023-12-05 Seed Health, Inc. Probiotic to enhance an individual's skin microbiome
US11980643B2 (en) 2013-12-20 2024-05-14 Seed Health, Inc. Method and system to modify an individual's gut-brain axis to provide neurocognitive protection
US11826388B2 (en) 2013-12-20 2023-11-28 Seed Health, Inc. Topical application of Lactobacillus crispatus to ameliorate barrier damage and inflammation
US12005085B2 (en) 2013-12-20 2024-06-11 Seed Health, Inc. Probiotic method and composition for maintaining a healthy vaginal microbiome
US10774338B2 (en) 2014-01-16 2020-09-15 The Regents Of The University Of California Generation of heritable chimeric plant traits
US20170159065A1 (en) 2014-07-08 2017-06-08 Vib Vzw Means and methods to increase plant yield
AU2015288157A1 (en) * 2014-07-11 2017-01-19 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
WO2016019144A2 (en) 2014-07-30 2016-02-04 Sangamo Biosciences, Inc. Gene correction of scid-related genes in hematopoietic stem and progenitor cells
MX2017002930A (es) 2014-09-12 2017-06-06 Du Pont Generacion de sitios de integracion especifica de sitio para loci de rasgos complejos en maiz y soja, y metodos de uso.
WO2016119703A1 (zh) 2015-01-27 2016-08-04 中国科学院遗传与发育生物学研究所 一种通过基因瞬时表达对整株植物定点改造的方法
BR112017017260A2 (pt) 2015-03-27 2018-04-17 Du Pont construções de dna, vetor, célula, plantas, semente, método de expressão de rna e método de modificação de local alvo
EP3095870A1 (en) 2015-05-19 2016-11-23 Kws Saat Se Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom
AU2016309392A1 (en) * 2015-08-14 2018-02-22 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Method for obtaining glyphosate-resistant rice by site-directed nucleotide substitution
EP3344643A4 (en) 2015-09-04 2019-05-01 Qiagen Sciences LLC METHODS FOR CO-ISOLATION OF PROTEINS AND NUCLEIC ACIDS
US10933128B2 (en) 2015-11-30 2021-03-02 Joseph E. Kovarik Method and system for protecting honey bees from pesticides
US11529412B2 (en) 2015-11-30 2022-12-20 Seed Health, Inc. Method and system for protecting honey bees from pesticides
US10568916B2 (en) 2015-11-30 2020-02-25 Joseph E. Kovarik Method and system for protecting honey bees, bats and butterflies from neonicotinoid pesticides
US10675347B2 (en) 2015-11-30 2020-06-09 Joseph E. Kovarik Method and system for protecting honey bees from fipronil pesticides
US10086024B2 (en) 2015-11-30 2018-10-02 Joseph E. Kovarik Method and system for protecting honey bees, bats and butterflies from neonicotinoid pesticides
US11098033B2 (en) 2016-05-24 2021-08-24 Indiana University Research And Technology Corporation Ku inhibitors and their use
CN105925590B (zh) * 2016-06-18 2019-05-17 北京大北农生物技术有限公司 除草剂抗性蛋白质、其编码基因及用途
EP3497213B1 (en) 2016-08-09 2023-10-18 Vib Vzw Cellulose synthase inhibitors and mutant plants
US11020492B2 (en) 2016-10-31 2021-06-01 Sangamo Therapeutics, Inc. Gene correction of SCID-related genes in hematopoietic stem and progenitor cells
CN106434635A (zh) * 2016-11-03 2017-02-22 云南省热带作物科学研究所 一种提高琴叶风吹楠dna提取质量的方法
WO2018204469A2 (en) 2017-05-03 2018-11-08 Sangamo Therapeutics, Inc. Methods and compositions for modification of a cystic fibrosis transmembrane conductance regulator (cftr) gene
KR20200079539A (ko) 2017-11-09 2020-07-03 상가모 테라퓨틱스, 인코포레이티드 시토카인 유도성 sh2-함유 단백질 (cish) 유전자의 유전자 변형
WO2019165168A1 (en) 2018-02-23 2019-08-29 Pioneer Hi-Bred International, Inc. Novel cas9 orthologs
US11421007B2 (en) 2018-04-18 2022-08-23 Sangamo Therapeutics, Inc. Zinc finger protein compositions for modulation of huntingtin (Htt)
US11690921B2 (en) 2018-05-18 2023-07-04 Sangamo Therapeutics, Inc. Delivery of target specific nucleases
GB201809273D0 (en) 2018-06-06 2018-07-25 Vib Vzw Novel mutant plant cinnamoyl-coa reductase proteins
GB201820109D0 (en) 2018-12-11 2019-01-23 Vib Vzw Plants with a lignin trait and udp-glycosyltransferase mutation
CN113166744A (zh) 2018-12-14 2021-07-23 先锋国际良种公司 用于基因组编辑的新颖crispr-cas系统
CN110904143A (zh) * 2019-09-12 2020-03-24 黑龙江省农业科学院耕作栽培研究所 一种多功能抗草甘膦水稻转化载体pCDMAR-epsps及其构建方法和应用
GB202007577D0 (en) 2020-05-21 2020-07-08 Oxford Genetics Ltd Hdr enhancers
WO2021234388A1 (en) 2020-05-21 2021-11-25 Oxford Genetics Limited Hdr enhancers
US20230183751A1 (en) 2020-05-21 2023-06-15 Oxford Genetics Limited Hdr enhancers
GB202007578D0 (en) 2020-05-21 2020-07-08 Univ Oxford Innovation Ltd Hdr enhancers
TW202305118A (zh) 2021-03-23 2023-02-01 美商艾歐凡斯生物治療公司 腫瘤浸潤淋巴球之ｃｉｓｈ基因編輯及其在免疫療法中之用途
WO2023131616A1 (en) 2022-01-05 2023-07-13 Vib Vzw Means and methods to increase abiotic stress tolerance in plants
WO2023131637A1 (en) 2022-01-06 2023-07-13 Vib Vzw Improved silage grasses
WO2023144199A1 (en) 2022-01-26 2023-08-03 Vib Vzw Plants having reduced levels of bitter taste metabolites

Family Cites Families (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1313830C (en) 1985-08-07 1993-02-23 Dilip Maganlal Shah Glyphosate-resistant plants
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
US5422251A (en) 1986-11-26 1995-06-06 Princeton University Triple-stranded nucleic acids
US4971908A (en) 1987-05-26 1990-11-20 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase
US5312910A (en) 1987-05-26 1994-05-17 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase
US5145783A (en) 1987-05-26 1992-09-08 Monsanto Company Glyphosate-tolerant 5-endolpyruvyl-3-phosphoshikimate synthase
US5176996A (en) 1988-12-20 1993-01-05 Baylor College Of Medicine Method for making synthetic oligonucleotides which bind specifically to target sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use
US5310667A (en) 1989-07-17 1994-05-10 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
AU655197B2 (en) 1990-06-25 1994-12-08 Monsanto Technology Llc Glyphosate tolerant plants
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US5866775A (en) 1990-09-28 1999-02-02 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
US5420032A (en) 1991-12-23 1995-05-30 Universitge Laval Homing endonuclease which originates from chlamydomonas eugametos and recognizes and cleaves a 15, 17 or 19 degenerate double stranded nucleotide sequence
US5487994A (en) 1992-04-03 1996-01-30 The Johns Hopkins University Insertion and deletion mutants of FokI restriction endonuclease
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US5356802A (en) 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5792632A (en) 1992-05-05 1998-08-11 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
EP0604662B1 (en) 1992-07-07 2008-06-18 Japan Tobacco Inc. Method of transforming monocotyledon
EP0682699B1 (en) 1993-02-12 2003-05-07 The Johns-Hopkins University FUNCTIONAL DOMAINS IN $i(FLAVOBACTERIUM OKEANOKOITES) (FOKI) RESTRICTION ENDONUCLEASE
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US6242568B1 (en) 1994-01-18 2001-06-05 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
DE69534629D1 (de) 1994-01-18 2005-12-29 Scripps Research Inst Derivate von zinkfingerproteinen und methoden
US5585245A (en) 1994-04-22 1996-12-17 California Institute Of Technology Ubiquitin-based split protein sensor
GB9824544D0 (en) * 1998-11-09 1999-01-06 Medical Res Council Screening system
USRE39229E1 (en) 1994-08-20 2006-08-08 Gendaq Limited Binding proteins for recognition of DNA
US7262055B2 (en) * 1998-08-25 2007-08-28 Gendaq Limited Regulated gene expression in plants
US5789538A (en) 1995-02-03 1998-08-04 Massachusetts Institute Of Technology Zinc finger proteins with high affinity new DNA binding specificities
US5925523A (en) 1996-08-23 1999-07-20 President & Fellows Of Harvard College Intraction trap assay, reagents and uses thereof
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
GB2338237B (en) 1997-02-18 2001-02-28 Actinova Ltd In vitro peptide or protein expression library
US6342345B1 (en) 1997-04-02 2002-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
US6040497A (en) 1997-04-03 2000-03-21 Dekalb Genetics Corporation Glyphosate resistant maize lines
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
GB9710807D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
US6303848B1 (en) 1998-01-16 2001-10-16 Large Scale Biology Corporation Method for conferring herbicide, pest, or disease resistance in plant hosts
AR014491A1 (es) 1998-01-29 2001-02-28 Dow Agrosciences Llc Metodo para obtener plantas transgenicas fertiles de gossypium hirsutum.
US6410248B1 (en) 1998-01-30 2002-06-25 Massachusetts Institute Of Technology General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
WO1999045132A1 (en) 1998-03-02 1999-09-10 Massachusetts Institute Of Technology Poly zinc finger proteins with improved linkers
JP2002506640A (ja) * 1998-03-17 2002-03-05 ジェンダック・リミテッド 核酸結合タンパク質
US6750377B1 (en) 1998-06-19 2004-06-15 Advanta Technology Ltd. Method of breeding glyphosate resistant plants
US6140081A (en) 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN
US6599692B1 (en) 1999-09-14 2003-07-29 Sangamo Bioscience, Inc. Functional genomics using zinc finger proteins
US6503717B2 (en) * 1999-12-06 2003-01-07 Sangamo Biosciences, Inc. Methods of using randomized libraries of zinc finger proteins for the identification of gene function
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US7030215B2 (en) * 1999-03-24 2006-04-18 Sangamo Biosciences, Inc. Position dependent recognition of GNN nucleotide triplets by zinc fingers
US6794136B1 (en) 2000-11-20 2004-09-21 Sangamo Biosciences, Inc. Iterative optimization in the design of binding proteins
WO2000066748A1 (en) 1999-04-29 2000-11-09 Syngenta Limited Herbicide resistant plants
PT1240340E (pt) 1999-12-16 2012-08-01 Monsanto Technology Llc Construções de dna para expressão de polipéptidos heterólogos em plantas
AU2001226935B2 (en) 2000-01-24 2006-06-22 Gendaq Limited Nucleic acid binding polypeptides characterized by flexible linkers connected nucleic acid binding modules
WO2001059450A2 (en) * 2000-02-08 2001-08-16 Sangamo Biosciences, Inc. Cells expressing zinc finger protein for drug discovery
US20020061512A1 (en) 2000-02-18 2002-05-23 Kim Jin-Soo Zinc finger domains and methods of identifying same
DE60111613T2 (de) 2000-03-09 2006-05-18 Monsanto Technology Llc. Verfahren zum herstellen von glyphosat-toleranten pflanzen
AU2001253914B2 (en) 2000-04-28 2006-06-08 Sangamo Therapeutics, Inc. Targeted modification of chromatin structure
EP1276865B1 (en) 2000-04-28 2014-05-21 Sangamo BioSciences, Inc. Methods for binding an exogenous molecule to cellular chromatin
US7217509B2 (en) 2000-04-28 2007-05-15 Sangamo Biosciences, Inc. Databases of regulatory sequences; methods of making and using same
WO2001088197A2 (en) 2000-05-16 2001-11-22 Massachusetts Institute Of Technology Methods and compositions for interaction trap assays
IL154059A0 (en) * 2000-07-21 2003-07-31 Syngenta Participations Ag Zinc finger domain recognition code and uses thereof
JP2002060786A (ja) 2000-08-23 2002-02-26 Kao Corp 硬質表面用殺菌防汚剤
AU2001287862B2 (en) * 2000-09-29 2006-12-14 Syngenta Limited Herbicide resistant plants
US9234187B2 (en) 2001-01-22 2016-01-12 Sangamo Biosciences, Inc. Modified zinc finger binding proteins
GB0108491D0 (en) 2001-04-04 2001-05-23 Gendaq Ltd Engineering zinc fingers
JP2005500061A (ja) * 2001-08-20 2005-01-06 ザ スクリップス リサーチ インスティテュート Ｃｎｎについての亜鉛フィンガー結合ドメイン
US7262054B2 (en) 2002-01-22 2007-08-28 Sangamo Biosciences, Inc. Zinc finger proteins for DNA binding and gene regulation in plants
AU2003251286B2 (en) 2002-01-23 2007-08-16 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
JP3873771B2 (ja) 2002-02-22 2007-01-24 ソニー株式会社 半導体装置の製造方法
EP2368982A3 (en) * 2002-03-21 2011-10-12 Sangamo BioSciences, Inc. Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
CN1330762C (zh) 2002-05-10 2007-08-08 北京大学 新的草甘膦耐受型5－烯醇丙酮酰莽草酸－3－磷酸合酶及其编码基因
US7045684B1 (en) 2002-08-19 2006-05-16 Mertec, Llc Glyphosate-resistant plants
WO2004037977A2 (en) 2002-09-05 2004-05-06 California Institute Of Thechnology Use of chimeric nucleases to stimulate gene targeting
AU2004213818B2 (en) * 2003-02-18 2008-06-05 Monsanto Technology Llc Glyphosate resistant class I 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS)
WO2005014820A1 (fr) 2003-08-08 2005-02-17 Si Chuan Heben Biotic Engineering Co. Ltd. 5-enolpyruvyl-3-phosphoshikimate synthase a bioresistance eleve au glyphosate et sequence de codage
CA2534296C (en) 2003-08-08 2013-03-26 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US20070134796A1 (en) 2005-07-26 2007-06-14 Sangamo Biosciences, Inc. Targeted integration and expression of exogenous nucleic acid sequences
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
ATE418346T1 (de) 2004-04-08 2009-01-15 Sangamo Biosciences Inc Zusammensetzungen zur behandlung von neuropathischen und neurodegenerativen erkrankungen
US20060063231A1 (en) 2004-09-16 2006-03-23 Sangamo Biosciences, Inc. Compositions and methods for protein production
WO2007062422A2 (en) * 2005-11-28 2007-05-31 The Scripps Research Institute Zinc finger binding domains for tnn
AU2007334468B2 (en) 2006-12-14 2013-02-07 Corteva Agriscience Llc Optimized non-canonical zinc finger proteins

Also Published As

Publication number Publication date
ES2585157T3 (es) 2016-10-04
BRPI0817560A2 (pt) 2015-07-14
JP5702144B2 (ja) 2015-04-15
WO2009042164A1 (en) 2009-04-02
CN101878307B (zh) 2017-07-28
EP2205749B1 (en) 2016-05-18
CA2699769C (en) 2020-08-18
EP3072973B1 (en) 2018-11-28
SI2205749T1 (sl) 2016-09-30
US20150064790A1 (en) 2015-03-05
US10344289B2 (en) 2019-07-09
TW200925276A (en) 2009-06-16
CN101878307A (zh) 2010-11-03
EP2205749A1 (en) 2010-07-14
AU2008305568B2 (en) 2013-11-21
CA2937438A1 (en) 2009-04-02
TWI424062B (zh) 2014-01-21
BRPI0817560B1 (pt) 2022-09-20
JP6382890B2 (ja) 2018-08-29
CA2699769A1 (en) 2009-04-02
US20130145503A1 (en) 2013-06-06
CA2937438C (en) 2020-07-07
JP6046677B2 (ja) 2016-12-21
US8399218B2 (en) 2013-03-19
JP2016187351A (ja) 2016-11-04
EP3072973A1 (en) 2016-09-28
JP2015037406A (ja) 2015-02-26
AR068559A1 (es) 2009-11-18
HUE029916T2 (en) 2017-04-28
US8889390B2 (en) 2014-11-18
HRP20161004T1 (hr) 2016-10-21
AU2008305568A1 (en) 2009-04-02
JP2010539930A (ja) 2010-12-24
PT2205749T (pt) 2016-07-20
US20090205083A1 (en) 2009-08-13

Similar Documents

Publication Publication Date Title
DK2205749T3 (da) Modificerede zinkfingerproteiner, som er målrettet mod 5-enolpyruvylshikimat-3-phosphat-syntasegener
US10669551B2 (en) Zinc finger nuclease-mediated homologous recombination
AU2017216446B2 (en) Zinc finger nuclease-mediated homologous recombination
AU2013216688B2 (en) Zinc finger nuclease-mediated homologous recombination