DK217985A - Rekombinant-fusionsproteiner - Google Patents

Rekombinant-fusionsproteiner Download PDF

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Publication number
DK217985A
DK217985A DK217985A DK217985A DK217985A DK 217985 A DK217985 A DK 217985A DK 217985 A DK217985 A DK 217985A DK 217985 A DK217985 A DK 217985A DK 217985 A DK217985 A DK 217985A
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DK
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Prior art keywords
factor
vector
protein
cleavage site
product
Prior art date
Application number
DK217985A
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English (en)
Other versions
DK171278B1 (da
DK217985D0 (da
Inventor
Kiyoshi Nagai
Hans Christian Thoegersen
Original Assignee
Celltech Ltd
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Publication date
Application filed by Celltech Ltd filed Critical Celltech Ltd
Publication of DK217985D0 publication Critical patent/DK217985D0/da
Publication of DK217985A publication Critical patent/DK217985A/da
Application granted granted Critical
Publication of DK171278B1 publication Critical patent/DK171278B1/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12N9/1033—Chloramphenicol O-acetyltransferase (2.3.1.28)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4716—Muscle proteins, e.g. myosin, actin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DK217985A 1984-05-16 1985-05-15 DNA-Sekvens, vektor omfattende en sådan sekvens, værtsorganisme transformeret med en sådan vektor og fremgangmåde til fremstilling af et protein eller peptidprodukt ved hjælp deraf DK171278B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB848412517A GB8412517D0 (en) 1984-05-16 1984-05-16 Recombinant fusion proteins
GB8412517 1984-05-16

Publications (3)

Publication Number Publication Date
DK217985D0 DK217985D0 (da) 1985-05-15
DK217985A true DK217985A (da) 1985-11-17
DK171278B1 DK171278B1 (da) 1996-08-19

Family

ID=10561052

Family Applications (1)

Application Number Title Priority Date Filing Date
DK217985A DK171278B1 (da) 1984-05-16 1985-05-15 DNA-Sekvens, vektor omfattende en sådan sekvens, værtsorganisme transformeret med en sådan vektor og fremgangmåde til fremstilling af et protein eller peptidprodukt ved hjælp deraf

Country Status (9)

Country Link
US (1) US6010883A (da)
EP (1) EP0161937B1 (da)
JP (1) JPH074253B2 (da)
AT (1) ATE81529T1 (da)
AU (1) AU585857B2 (da)
CA (1) CA1340381C (da)
DE (1) DE3586750T2 (da)
DK (1) DK171278B1 (da)
GB (2) GB8412517D0 (da)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8412517D0 (en) * 1984-05-16 1984-06-20 Nagai K Recombinant fusion proteins
US5411873A (en) * 1984-05-29 1995-05-02 Genencor, Inc. Process for producing heterologous polypeptides
US5087564A (en) * 1985-06-20 1992-02-11 Monsanto Company Release of recombinant peptides from polypeptides using V8 endopeptidase
EP0207044A1 (en) * 1985-06-20 1986-12-30 Monsanto Company Release of peptides from polypeptides
US5496924A (en) * 1985-11-27 1996-03-05 Hoechst Aktiengesellschaft Fusion protein comprising an interleukin-2 fragment ballast portion
DE3636903A1 (de) * 1985-12-21 1987-07-02 Hoechst Ag Fusionsproteine mit eukaryotischem ballastanteil
US5089406A (en) * 1987-01-07 1992-02-18 Allied-Signal Inc. Method of producing a gene cassette coding for polypeptides with repeating amino acid sequences
CA1340522C (en) * 1987-03-10 1999-05-04 Heinz Dobeli Fusion proteins containing neighbouring histidines for improved purification
US5013653A (en) 1987-03-20 1991-05-07 Creative Biomolecules, Inc. Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
US5215896A (en) * 1987-03-20 1993-06-01 Creative Biomolecules, Inc. Leader sequences for the production of recombinant proteins
DE3731874A1 (de) * 1987-09-18 1989-03-30 Schering Ag Verfahren zur herstellung von peptiden durch spezifische spaltung von gentechnisch gewonnenen fusionsproteinen mit collagenasen
IT1223577B (it) * 1987-12-22 1990-09-19 Eniricerche Spa Procedimento migliorato per la preparazione dell'ormone della crescita umano naturale in forma pura
DE3804148A1 (de) * 1988-02-11 1989-08-24 Andreas Dr Plueckthun Verfahren zur spaltung von fusionsproteinen
US5545727A (en) 1989-05-10 1996-08-13 Somatogen, Inc. DNA encoding fused di-alpha globins and production of pseudotetrameric hemoglobin
US5599907A (en) * 1989-05-10 1997-02-04 Somatogen, Inc. Production and use of multimeric hemoglobins
HUT61591A (en) 1989-05-10 1993-01-28 Somatogen Inc Process for producing haemoglobin and its analogs
US5580560A (en) * 1989-11-13 1996-12-03 Novo Nordisk A/S Modified factor VII/VIIa
GB8927722D0 (en) * 1989-12-07 1990-02-07 British Bio Technology Proteins and nucleic acids
DE4009268A1 (de) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
WO1992000318A1 (en) * 1990-06-25 1992-01-09 Oncogene Science, Inc. Tissue-derived tumor growth inhibitors, methods for preparation and uses thereof
US6514730B1 (en) 1991-03-21 2003-02-04 Consortium für elektrochemische Industrie GmbH Secretion of hirudin derivatives
JP3277523B2 (ja) * 1991-09-13 2002-04-22 株式会社日立製作所 因子ＸａリンカーＤＮＡ
DE69226197T2 (de) * 1991-11-08 1999-02-11 Somatogen Inc Hämoglobine als arzneimittelabgabesystem
DE69432744T2 (de) * 1993-02-04 2004-03-25 Borean Pharma A/S Verbessertes verfahren zur rückfaltung der proteine
GB9406974D0 (en) * 1994-04-08 1994-06-01 Pharmaceutical Proteins Ltd Transgenic production
DE19653498C1 (de) * 1996-12-20 1998-07-23 Wolf M Prof Dr Bertling Klonierungsvektor-System mit Fusionssequenz
US20030207402A1 (en) * 1997-08-22 2003-11-06 Erhard Kopetzki Autocatalytically activatable zymogenic precursors of proteases and their use
JP2003520571A (ja) * 1999-06-02 2003-07-08 ノボザイムス アクティーゼルスカブ ポリペプチドの発現及び分泌のためのペクチン酸リアーゼ
IL149679A0 (en) * 1999-11-19 2002-11-10 Transkaryotic Therapies Inc Nucleic acid construct and methods utilizing the same
DE10122140A1 (de) * 2001-05-08 2002-11-28 Apotech Res & Dev Ltd Rekombinante Fusionsproteine und deren Trimere
ES2427974T3 (es) * 2003-04-23 2013-11-05 F. Hoffmann-La Roche Ag Corte de proteínas de fusión mediante la utilización de la proteasa granzima B
PL213309B1 (pl) 2006-02-09 2013-02-28 Polska Akademia Nauk Inst Biochemii I Biofizyki Sposób hydrolizy wiazania peptydowego
US8099316B2 (en) * 2007-07-09 2012-01-17 Velti Plc Mobile device marketing and advertising platforms, methods, and systems
ES2399088T3 (es) 2007-11-01 2013-03-25 Perseid Therapeutics Llc Polipéptidos y ácidos nucleicos inmunosupresores
US8053222B2 (en) * 2009-02-12 2011-11-08 Academia Sinica, Taiwan Protein expression system involving mutated severe respiratory syndrome-associated coronavirus 3C-like protease
US9913890B2 (en) * 2012-06-22 2018-03-13 Pharma Green Llc Methods and compositions for emergency post-infection treatment of anthrax

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4366246A (en) * 1977-11-08 1982-12-28 Genentech, Inc. Method for microbial polypeptide expression
IL60184A (en) * 1979-05-31 1984-05-31 Schering Ag Process for the specific cleavage of protein sequences from proteins
CA1200773A (en) * 1980-02-29 1986-02-18 William J. Rutter Expression linkers
US4532207A (en) * 1982-03-19 1985-07-30 G. D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
EP0095361B1 (en) * 1982-05-25 1989-07-26 Eli Lilly And Company Cloning vectors for expression of exogenous protein
WO1984001173A1 (en) * 1982-09-15 1984-03-29 Immuno Nuclear Corp Production of mature proteins in transformed yeast
ATE30246T1 (de) * 1982-12-10 1987-10-15 Nordisk Gentofte Verfahren zur herstellung reifer proteine aus schmelzproteinen hergestellt in pro- oder eukaryotischen zellen.
SE8300693L (sv) * 1983-02-09 1984-08-10 Sven Lofdahl Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta
GB2140810B (en) * 1983-05-24 1987-07-08 Celltech Ltd Polypeptide and protein products, and process for their production
GB8314362D0 (en) * 1983-05-24 1983-06-29 Celltech Ltd Polypeptide and protein products
GB2142337A (en) * 1983-07-01 1985-01-16 Celltech Ltd Preparation of lingual lipase protein
GB8412517D0 (en) * 1984-05-16 1984-06-20 Nagai K Recombinant fusion proteins
US4713482A (en) * 1985-03-26 1987-12-15 Ciba-Geigy Corporation Maleic or phthalic acid half esters of alkoxylated fatty amines
ES2000858A6 (es) * 1985-08-12 1988-03-16 Syntex Inc Un metodo para producir un vector de expresion bacteriano para la expresion de proteinas de fusion

Also Published As

Publication number Publication date
CA1340381C (en) 1999-02-09
DE3586750T2 (de) 1993-04-22
DK171278B1 (da) 1996-08-19
DE3586750D1 (de) 1992-11-19
AU4247485A (en) 1985-11-21
ATE81529T1 (de) 1992-10-15
AU585857B2 (en) 1989-06-29
GB2160206A (en) 1985-12-18
DK217985D0 (da) 1985-05-15
JPS61135591A (ja) 1986-06-23
US6010883A (en) 2000-01-04
GB8412517D0 (en) 1984-06-20
JPH074253B2 (ja) 1995-01-25
GB8512333D0 (en) 1985-06-19
GB2160206B (en) 1987-11-11
EP0161937A2 (en) 1985-11-21
EP0161937B1 (en) 1992-10-14
EP0161937A3 (en) 1987-10-07

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B1 Patent granted (law 1993)
PUP Patent expired