ATE30246T1 - Verfahren zur herstellung reifer proteine aus schmelzproteinen hergestellt in pro- oder eukaryotischen zellen. - Google Patents
Verfahren zur herstellung reifer proteine aus schmelzproteinen hergestellt in pro- oder eukaryotischen zellen.Info
- Publication number
- ATE30246T1 ATE30246T1 AT84900036T AT84900036T ATE30246T1 AT E30246 T1 ATE30246 T1 AT E30246T1 AT 84900036 T AT84900036 T AT 84900036T AT 84900036 T AT84900036 T AT 84900036T AT E30246 T1 ATE30246 T1 AT E30246T1
- Authority
- AT
- Austria
- Prior art keywords
- pro
- amino acid
- protein
- proteins
- eukaryotic cells
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 6
- 108090000623 proteins and genes Proteins 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 2
- 238000002844 melting Methods 0.000 title 1
- 230000008018 melting Effects 0.000 title 1
- 150000001413 amino acids Chemical class 0.000 abstract 3
- 102000004400 Aminopeptidases Human genes 0.000 abstract 2
- 108090000915 Aminopeptidases Proteins 0.000 abstract 2
- 238000003776 cleavage reaction Methods 0.000 abstract 2
- 230000007017 scission Effects 0.000 abstract 2
- 102000002265 Human Growth Hormone Human genes 0.000 abstract 1
- 108010000521 Human Growth Hormone Proteins 0.000 abstract 1
- 239000000854 Human Growth Hormone Substances 0.000 abstract 1
- 102000002704 Leucyl aminopeptidase Human genes 0.000 abstract 1
- 108010004098 Leucyl aminopeptidase Proteins 0.000 abstract 1
- PYUSHNKNPOHWEZ-YFKPBYRVSA-N N-formyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC=O PYUSHNKNPOHWEZ-YFKPBYRVSA-N 0.000 abstract 1
- 108010076181 Proinsulin Proteins 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 125000000539 amino acid group Chemical group 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 1
- 108020001507 fusion proteins Proteins 0.000 abstract 1
- 102000037865 fusion proteins Human genes 0.000 abstract 1
- 238000010353 genetic engineering Methods 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 abstract 1
- 210000001236 prokaryotic cell Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2462—Lysozyme (3.2.1.17)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/545—IL-1
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Diabetes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK549382 | 1982-12-10 | ||
| PCT/DK1983/000118 WO1984002351A1 (en) | 1982-12-10 | 1983-12-09 | A process for preparing ripe proteins from fusion proteins, synthetized in pro- or eukaryotic cells |
| EP84900036A EP0127658B1 (de) | 1982-12-10 | 1983-12-09 | Verfahren zur herstellung reifer proteine aus schmelzproteinen hergestellt in pro- oder eukaryotischen zellen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE30246T1 true ATE30246T1 (de) | 1987-10-15 |
Family
ID=8142905
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT84900036T ATE30246T1 (de) | 1982-12-10 | 1983-12-09 | Verfahren zur herstellung reifer proteine aus schmelzproteinen hergestellt in pro- oder eukaryotischen zellen. |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0127658B1 (de) |
| JP (1) | JPS60500043A (de) |
| AT (1) | ATE30246T1 (de) |
| AU (1) | AU2331784A (de) |
| DE (1) | DE3374070D1 (de) |
| DK (1) | DK344884A (de) |
| WO (1) | WO1984002351A1 (de) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK55685A (da) * | 1985-02-07 | 1986-08-08 | Nordisk Gentofte | Enzym eller enzymkompleks med proteolytisk aktivitet |
| GB8412517D0 (en) * | 1984-05-16 | 1984-06-20 | Nagai K | Recombinant fusion proteins |
| DE3588249T2 (de) * | 1984-08-16 | 2004-05-06 | Savient Pharmaceuticals, Inc. | Methode zur Herstellung von menschlichen Wachstumshormonen |
| US5763215A (en) * | 1984-08-16 | 1998-06-09 | Bio-Technology General Corporation | Method of removing N-terminal amino acid residues from eucaryotic polypeptide analogs and polypeptides produced thereby |
| WO1986007380A1 (en) * | 1985-06-04 | 1986-12-18 | Takeda Chemical Industries, Ltd. | Process for preparing polypeptide |
| US4933288A (en) * | 1986-11-21 | 1990-06-12 | Cetus Corporation | Use of a modified soluble Pseudomonas exotoxin A in immunoconjugates |
| JPH01181796A (ja) * | 1988-01-14 | 1989-07-19 | Ajinomoto Co Inc | 組換えｄｎａ法を用いたポリペプチドの製造法 |
| DE3811921A1 (de) * | 1988-04-09 | 1989-10-19 | Hoechst Ag | Verfahren zur herstellung von proteinen, die n-terminal mit prolin beginnen |
| DE4039415A1 (de) | 1990-02-03 | 1991-08-08 | Boehringer Mannheim Gmbh | Verfahren zur herstellung rekombinanter proteine ohne n-terminalen methioninrest |
| JP2000515017A (ja) * | 1996-07-19 | 2000-11-14 | モンサント カンパニー | バクテリア発現系におけるｆ―Ｍｅｔペプチドの脱ホルミル化 |
| US6794159B1 (en) | 1999-04-30 | 2004-09-21 | Pharmacia Corporation | Method of removing n-terminal alanine residues from polypeptides with aeromonas aminopeptidase |
| US6743600B1 (en) | 1999-04-30 | 2004-06-01 | Monsanto Technologies Llc | Method of removing N-terminal alanine residues from polypeptides with Aeromonas aminopeptidase |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL60184A (en) * | 1979-05-31 | 1984-05-31 | Schering Ag | Process for the specific cleavage of protein sequences from proteins |
| US4532207A (en) * | 1982-03-19 | 1985-07-30 | G. D. Searle & Co. | Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase |
-
1983
- 1983-12-09 WO PCT/DK1983/000118 patent/WO1984002351A1/en active IP Right Grant
- 1983-12-09 JP JP59500221A patent/JPS60500043A/ja active Pending
- 1983-12-09 AU AU23317/84A patent/AU2331784A/en not_active Abandoned
- 1983-12-09 AT AT84900036T patent/ATE30246T1/de not_active IP Right Cessation
- 1983-12-09 DE DE8484900036T patent/DE3374070D1/de not_active Expired
- 1983-12-09 EP EP84900036A patent/EP0127658B1/de not_active Expired
-
1984
- 1984-07-13 DK DK344884A patent/DK344884A/da not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| DE3374070D1 (en) | 1987-11-19 |
| DK344884D0 (da) | 1984-07-13 |
| AU2331784A (en) | 1984-07-05 |
| EP0127658B1 (de) | 1987-10-14 |
| WO1984002351A1 (en) | 1984-06-21 |
| EP0127658A1 (de) | 1984-12-12 |
| DK344884A (da) | 1984-07-13 |
| JPS60500043A (ja) | 1985-01-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| REN | Ceased due to non-payment of the annual fee |