ATE81529T1 - Rekombinante fusionsproteine. - Google Patents

Rekombinante fusionsproteine.

Info

Publication number
ATE81529T1
ATE81529T1 AT85303414T AT85303414T ATE81529T1 AT E81529 T1 ATE81529 T1 AT E81529T1 AT 85303414 T AT85303414 T AT 85303414T AT 85303414 T AT85303414 T AT 85303414T AT E81529 T1 ATE81529 T1 AT E81529T1
Authority
AT
Austria
Prior art keywords
factor
vector
protein
cleavage site
product
Prior art date
Application number
AT85303414T
Other languages
English (en)
Inventor
Kiyoshi Nagai
Hans Christian Thogersen
Original Assignee
Celltech Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celltech Ltd filed Critical Celltech Ltd
Application granted granted Critical
Publication of ATE81529T1 publication Critical patent/ATE81529T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12N9/1033—Chloramphenicol O-acetyltransferase (2.3.1.28)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4716—Muscle proteins, e.g. myosin, actin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT85303414T 1984-05-16 1985-05-15 Rekombinante fusionsproteine. ATE81529T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB848412517A GB8412517D0 (en) 1984-05-16 1984-05-16 Recombinant fusion proteins
EP85303414A EP0161937B1 (de) 1984-05-16 1985-05-15 Rekombinante Fusionsproteine

Publications (1)

Publication Number Publication Date
ATE81529T1 true ATE81529T1 (de) 1992-10-15

Family

ID=10561052

Family Applications (1)

Application Number Title Priority Date Filing Date
AT85303414T ATE81529T1 (de) 1984-05-16 1985-05-15 Rekombinante fusionsproteine.

Country Status (9)

Country Link
US (1) US6010883A (de)
EP (1) EP0161937B1 (de)
JP (1) JPH074253B2 (de)
AT (1) ATE81529T1 (de)
AU (1) AU585857B2 (de)
CA (1) CA1340381C (de)
DE (1) DE3586750T2 (de)
DK (1) DK171278B1 (de)
GB (2) GB8412517D0 (de)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8412517D0 (en) * 1984-05-16 1984-06-20 Nagai K Recombinant fusion proteins
US5411873A (en) * 1984-05-29 1995-05-02 Genencor, Inc. Process for producing heterologous polypeptides
US5087564A (en) * 1985-06-20 1992-02-11 Monsanto Company Release of recombinant peptides from polypeptides using V8 endopeptidase
EP0207044A1 (de) * 1985-06-20 1986-12-30 Monsanto Company Freisetzung von Peptiden aus Polypeptiden
US5496924A (en) * 1985-11-27 1996-03-05 Hoechst Aktiengesellschaft Fusion protein comprising an interleukin-2 fragment ballast portion
DE3636903A1 (de) * 1985-12-21 1987-07-02 Hoechst Ag Fusionsproteine mit eukaryotischem ballastanteil
US5089406A (en) * 1987-01-07 1992-02-18 Allied-Signal Inc. Method of producing a gene cassette coding for polypeptides with repeating amino acid sequences
CA1340522C (en) * 1987-03-10 1999-05-04 Heinz Dobeli Fusion proteins containing neighbouring histidines for improved purification
US5215896A (en) * 1987-03-20 1993-06-01 Creative Biomolecules, Inc. Leader sequences for the production of recombinant proteins
US5013653A (en) * 1987-03-20 1991-05-07 Creative Biomolecules, Inc. Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
DE3731874A1 (de) * 1987-09-18 1989-03-30 Schering Ag Verfahren zur herstellung von peptiden durch spezifische spaltung von gentechnisch gewonnenen fusionsproteinen mit collagenasen
IT1223577B (it) * 1987-12-22 1990-09-19 Eniricerche Spa Procedimento migliorato per la preparazione dell'ormone della crescita umano naturale in forma pura
DE3804148A1 (de) * 1988-02-11 1989-08-24 Andreas Dr Plueckthun Verfahren zur spaltung von fusionsproteinen
US5545727A (en) 1989-05-10 1996-08-13 Somatogen, Inc. DNA encoding fused di-alpha globins and production of pseudotetrameric hemoglobin
IL94361A (en) 1989-05-10 1995-07-31 Somatogenetics Int Polypeptides, recombinant AND molecules and a method for the production of human hemoglobin-like protein
US5599907A (en) * 1989-05-10 1997-02-04 Somatogen, Inc. Production and use of multimeric hemoglobins
US5580560A (en) * 1989-11-13 1996-12-03 Novo Nordisk A/S Modified factor VII/VIIa
GB8927722D0 (en) * 1989-12-07 1990-02-07 British Bio Technology Proteins and nucleic acids
DE4009268A1 (de) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
EP0538398A4 (en) * 1990-06-25 1993-12-29 Oncogene Science, Inc. Tissue-derived tumor growth inhibitors, methods for preparation and uses thereof
US6514730B1 (en) 1991-03-21 2003-02-04 Consortium für elektrochemische Industrie GmbH Secretion of hirudin derivatives
JP3277523B2 (ja) * 1991-09-13 2002-04-22 株式会社日立製作所 因子ＸａリンカーＤＮＡ
EP0611306B1 (de) * 1991-11-08 1998-07-08 Somatogen, Inc. Hämoglobine als arzneimittelabgabesystem
ES2199959T3 (es) * 1993-02-04 2004-03-01 Borean Pharma A/S Procedimiento mejorado para el replegamiento de proteinas.
GB9406974D0 (en) * 1994-04-08 1994-06-01 Pharmaceutical Proteins Ltd Transgenic production
DE19653498C1 (de) * 1996-12-20 1998-07-23 Wolf M Prof Dr Bertling Klonierungsvektor-System mit Fusionssequenz
US20030207402A1 (en) * 1997-08-22 2003-11-06 Erhard Kopetzki Autocatalytically activatable zymogenic precursors of proteases and their use
EP1187925B1 (de) * 1999-06-02 2005-03-30 Novozymes A/S Pektat lyase-fusion zur expression und ausscheidung von polypeptiden
JP2003514532A (ja) * 1999-11-19 2003-04-22 トランスカーヨティック セラピーズ インコーポレイテッド 生成物の産生量を最適化するための核酸構築体
DE10122140A1 (de) * 2001-05-08 2002-11-28 Apotech Res & Dev Ltd Rekombinante Fusionsproteine und deren Trimere
JP4833056B2 (ja) * 2003-04-23 2011-12-07 アナフォア インコーポレイテッド グランザイムｂプロテアーゼを使用した融合タンパク質の開裂
PL213309B1 (pl) 2006-02-09 2013-02-28 Polska Akademia Nauk Inst Biochemii I Biofizyki Sposób hydrolizy wiazania peptydowego
CN101802860A (zh) * 2007-07-09 2010-08-11 维蒂公开股份有限公司 移动设备销售和广告平台、方法及系统
CN101998965B (zh) 2007-11-01 2014-03-12 安斯泰来制药有限公司 免疫抑制性多肽与核酸
US8053222B2 (en) * 2009-02-12 2011-11-08 Academia Sinica, Taiwan Protein expression system involving mutated severe respiratory syndrome-associated coronavirus 3C-like protease
US9913890B2 (en) * 2012-06-22 2018-03-13 Pharma Green Llc Methods and compositions for emergency post-infection treatment of anthrax

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4366246A (en) * 1977-11-08 1982-12-28 Genentech, Inc. Method for microbial polypeptide expression
IL60184A (en) * 1979-05-31 1984-05-31 Schering Ag Process for the specific cleavage of protein sequences from proteins
CA1200773A (en) * 1980-02-29 1986-02-18 William J. Rutter Expression linkers
US4532207A (en) * 1982-03-19 1985-07-30 G. D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
EP0095361B1 (de) * 1982-05-25 1989-07-26 Eli Lilly And Company Klonbildungsvektoren für die Expression von exogenem Protein
EP0119247A1 (de) * 1982-09-15 1984-09-26 Immuno Nuclear Corporation Herstellung reifer proteine in transformierter hefe
DE3374070D1 (en) * 1982-12-10 1987-11-19 Nordisk Gentofte A process for preparing ripe proteins from fusion proteins, synthetized in pro- or eukaryotic cells
SE8300693L (sv) * 1983-02-09 1984-08-10 Sven Lofdahl Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta
GB2140810B (en) * 1983-05-24 1987-07-08 Celltech Ltd Polypeptide and protein products, and process for their production
GB8314362D0 (en) * 1983-05-24 1983-06-29 Celltech Ltd Polypeptide and protein products
EP0131418A1 (de) * 1983-07-01 1985-01-16 Celltech Limited Proteine, pharmazeutische Zusammensetzungen, Gene, Vektoren, Wirtorganismen und Verfahren zu deren Herstellung
GB8412517D0 (en) * 1984-05-16 1984-06-20 Nagai K Recombinant fusion proteins
US4713482A (en) * 1985-03-26 1987-12-15 Ciba-Geigy Corporation Maleic or phthalic acid half esters of alkoxylated fatty amines
AU6104786A (en) * 1985-08-12 1987-02-19 Syntex (U.S.A.) Inc. Fusion proteins using trple gene

Also Published As

Publication number Publication date
US6010883A (en) 2000-01-04
AU585857B2 (en) 1989-06-29
EP0161937A3 (en) 1987-10-07
DK171278B1 (da) 1996-08-19
DE3586750D1 (de) 1992-11-19
DK217985A (da) 1985-11-17
GB8412517D0 (en) 1984-06-20
DE3586750T2 (de) 1993-04-22
GB8512333D0 (en) 1985-06-19
CA1340381C (en) 1999-02-09
DK217985D0 (da) 1985-05-15
AU4247485A (en) 1985-11-21
JPS61135591A (ja) 1986-06-23
EP0161937B1 (de) 1992-10-14
EP0161937A2 (de) 1985-11-21
JPH074253B2 (ja) 1995-01-25
GB2160206B (en) 1987-11-11
GB2160206A (en) 1985-12-18

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Legal Events

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UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee