DK2087105T3 - Delta 17-desaturase og anvendelse heraf ved fremstilling af flerumættede fedtsyrer - Google Patents

Delta 17-desaturase og anvendelse heraf ved fremstilling af flerumættede fedtsyrer Download PDF

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Publication number
DK2087105T3
DK2087105T3 DK07836170.6T DK07836170T DK2087105T3 DK 2087105 T3 DK2087105 T3 DK 2087105T3 DK 07836170 T DK07836170 T DK 07836170T DK 2087105 T3 DK2087105 T3 DK 2087105T3
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Denmark
Prior art keywords
desaturase
seq
leu
ala
acid
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DK07836170.6T
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Danish (da)
English (en)
Inventor
Zhixiong Xue
Quinn Qun Zhu
Narendra S Yadav
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Du Pont
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Publication of DK2087105T3 publication Critical patent/DK2087105T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DK07836170.6T 2006-10-30 2007-07-19 Delta 17-desaturase og anvendelse heraf ved fremstilling af flerumættede fedtsyrer DK2087105T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US85517706P 2006-10-30 2006-10-30
PCT/US2007/016490 WO2008054565A1 (en) 2006-10-30 2007-07-19 Δ17 desaturase and its use in making polyunsaturated fatty acids

Publications (1)

Publication Number Publication Date
DK2087105T3 true DK2087105T3 (da) 2015-05-26

Family

ID=39167662

Family Applications (1)

Application Number Title Priority Date Filing Date
DK07836170.6T DK2087105T3 (da) 2006-10-30 2007-07-19 Delta 17-desaturase og anvendelse heraf ved fremstilling af flerumættede fedtsyrer

Country Status (8)

Country Link
US (3) US7556949B2 (enExample)
EP (1) EP2087105B1 (enExample)
JP (1) JP5193216B2 (enExample)
CN (1) CN101617040B (enExample)
AU (1) AU2007314481B2 (enExample)
CA (1) CA2663807C (enExample)
DK (1) DK2087105T3 (enExample)
WO (1) WO2008054565A1 (enExample)

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US20110059496A1 (en) * 2003-06-25 2011-03-10 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
WO2010080388A1 (en) 2008-12-18 2010-07-15 E. I. Du Pont De Nemours And Company Reducing byproduction of malonates in a fermentation process
DK2442666T3 (da) 2009-06-16 2015-07-13 Du Pont Olier med højt indhold af eicosapentaensyre fra forbedrede og optimerede stammer af yarrowia lipolytica
CA2761589C (en) 2009-06-16 2017-12-19 E. I. Du Pont De Nemours And Company Improved optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
AU2010260227B2 (en) 2009-06-16 2015-01-29 E. I. Du Pont De Nemours And Company Improvement of long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases
US20110178105A1 (en) 2010-01-15 2011-07-21 E.I. Du Pont De Nemours And Company Clinical benefits of eicosapentaenoic acid in humans
BR122015020119B8 (pt) 2010-01-19 2021-05-04 Dsm Ip Assets Bv óleo microbiano, produtos alimentícios para um animal não humano e biomassa isolada
EP2561050A1 (en) 2010-04-22 2013-02-27 E.I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
US20120040076A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
US20120207912A1 (en) 2010-08-11 2012-08-16 E.I. Du Pont De Nemours And Company Aquaculture meat products
EP2603094A1 (en) 2010-08-11 2013-06-19 E.I. Du Pont De Nemours And Company A sustainable aquaculture feeding strategy
EP2609202A1 (en) 2010-08-26 2013-07-03 E.I. Du Pont De Nemours And Company Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids
CN103249834B (zh) 2010-08-26 2016-10-26 纳幕尔杜邦公司 生产高水平二十碳五烯酸的重组微生物宿主细胞
US8980589B2 (en) 2010-08-26 2015-03-17 E I Du Pont De Nemours And Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
WO2012043826A1 (ja) 2010-10-01 2012-04-05 国立大学法人九州大学 ストラメノパイルの形質転換方法
JP2014503216A (ja) 2010-12-30 2014-02-13 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー スクロース利用のための、ヤロウィア・リポリティカ（Ｙａｒｒｏｗｉａｌｉｐｏｌｙｔｉｃａ）におけるサッカロミセス・セレビシア（Ｓａｃｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅ）ＳＵＣ２遺伝子の使用
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
CN103841825B (zh) 2011-02-11 2017-03-22 纳幕尔杜邦公司 从微生物生物质中获取含脂质组合物的方法
US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
WO2013013211A1 (en) 2011-07-21 2013-01-24 Dsm Ip Assets B.V. Fatty acid compositions
US9005940B2 (en) 2012-12-21 2015-04-14 E I Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a Sou2 sorbitol utilization protein to modify lipid production in microbial cells
JP2016165226A (ja) * 2013-07-12 2016-09-15 国立大学法人京都大学 エイコサペンタエン酸を高含有する脂質の生産方法
WO2015029966A1 (ja) 2013-08-27 2015-03-05 国立大学法人京都大学 ω３不飽和脂肪酸酵素およびエイコサペンタエン酸の製造方法
JP2017195777A (ja) * 2014-08-29 2017-11-02 国立大学法人京都大学 エイコサペンタエン酸を高含有する脂質の生産方法
JP6026709B1 (ja) 2014-12-25 2016-11-16 国立大学法人京都大学 新規ω３不飽和脂肪酸酵素およびエイコサペンタエン酸の製造方法
CN106010993B (zh) * 2016-06-12 2019-07-05 福建师范大学 生产亚麻酸的圆红冬孢酵母及其制备方法
US11913046B2 (en) 2018-04-23 2024-02-27 Inbiose N.V. Increasing export of 2'fucosyllactose from microbial cells through the expression of a heterologous nucleic acid

Family Cites Families (15)

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Publication number Priority date Publication date Assignee Title
KR100446323B1 (ko) * 1998-10-23 2004-08-30 다이호야쿠힌고교 가부시키가이샤 Ｇｍ-95 물질, 이의 제조 방법 및 용도
WO2002011887A1 (en) * 2000-08-03 2002-02-14 Caliper Technologies Corp. Methods and devices for high throughput fluid delivery
US6635451B2 (en) 2001-01-25 2003-10-21 Abbott Laboratories Desaturase genes and uses thereof
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
WO2005049805A2 (en) 2003-11-14 2005-06-02 E.I. Dupont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast
EP1720988B1 (de) 2004-02-27 2011-10-12 BASF Plant Science GmbH Verfahren zur herstellung von ungesättigten omega-3-fettsäuren in transgenen organismen
WO2005083093A2 (de) * 2004-02-27 2005-09-09 Basf Plant Science Gmbh Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen pflanzen
US7198937B2 (en) 2004-11-04 2007-04-03 E. I. Du Pont De Nemours And Company Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7189559B2 (en) 2004-11-04 2007-03-13 E. I. Du Pont De Nemours And Company Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7192762B2 (en) 2004-11-04 2007-03-20 E. I. Du Pont De Nemours And Company Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
DE102005013779A1 (de) * 2005-03-22 2006-09-28 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten C20- und C22-Fettsäuren mit mindestens vier Doppelbindungen in transgenen Pflanzen
TWI258780B (en) * 2005-04-29 2006-07-21 Asustek Comp Inc Button structure
US7465793B2 (en) * 2006-04-20 2008-12-16 E.I. Du Pont De Nemours And Company Synthetic Δ17 desaturase derived from Phytophthora ramourum and its use in making polyunsaturated fatty acids
EP2500420B1 (en) 2006-08-24 2016-06-22 BASF Plant Science GmbH Pythium omega 3 desaturase with specificity to all omega 6 fatty acids longer than 18 carbon chains

Also Published As

Publication number Publication date
EP2087105A1 (en) 2009-08-12
US7556949B2 (en) 2009-07-07
AU2007314481A1 (en) 2008-05-08
WO2008054565A1 (en) 2008-05-08
US20090280996A1 (en) 2009-11-12
CA2663807C (en) 2018-05-01
EP2087105B1 (en) 2015-02-25
JP5193216B2 (ja) 2013-05-08
JP2010508019A (ja) 2010-03-18
US20080125326A1 (en) 2008-05-29
CN101617040B (zh) 2012-07-04
US20130011846A1 (en) 2013-01-10
CN101617040A (zh) 2009-12-30
AU2007314481B2 (en) 2012-11-29
US8241884B2 (en) 2012-08-14
CA2663807A1 (en) 2008-05-08

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