DK175538B1 - Fremgangsmåde til fremstilling af et alfa-amiderende enzym, genmanipuleret mikroorganisme eller værtscelle og rekombinant DNA-molekyle,............. - Google Patents

Fremgangsmåde til fremstilling af et alfa-amiderende enzym, genmanipuleret mikroorganisme eller værtscelle og rekombinant DNA-molekyle,............. Download PDF

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Publication number
DK175538B1
DK175538B1 DK198804489A DK448988A DK175538B1 DK 175538 B1 DK175538 B1 DK 175538B1 DK 198804489 A DK198804489 A DK 198804489A DK 448988 A DK448988 A DK 448988A DK 175538 B1 DK175538 B1 DK 175538B1
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DK
Denmark
Prior art keywords
enzyme
amidating
sequence
activity
host cell
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DK198804489A
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Danish (da)
English (en)
Other versions
DK448988A (da
DK448988D0 (da
Inventor
James P Gilligan
Barry N Jones
Arthur H Bertelsen
Nozer M Mehta
Original Assignee
Unigene Lab Inc
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Application filed by Unigene Lab Inc filed Critical Unigene Lab Inc
Publication of DK448988D0 publication Critical patent/DK448988D0/da
Publication of DK448988A publication Critical patent/DK448988A/da
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Publication of DK175538B1 publication Critical patent/DK175538B1/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/17—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced ascorbate as one donor, and incorporation of one atom of oxygen (1.14.17)
    • C12Y114/17003—Peptidylglycine monooxygenase (1.14.17.3)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Detergent Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Materials Applied To Surfaces To Minimize Adherence Of Mist Or Water (AREA)
DK198804489A 1987-08-14 1988-08-11 Fremgangsmåde til fremstilling af et alfa-amiderende enzym, genmanipuleret mikroorganisme eller værtscelle og rekombinant DNA-molekyle,............. DK175538B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/086,161 US6319685B1 (en) 1984-09-27 1987-08-14 Alpha-amidating enzyme compositions and processes for their production and use
US8616187 1987-08-14

Publications (3)

Publication Number Publication Date
DK448988D0 DK448988D0 (da) 1988-08-11
DK448988A DK448988A (da) 1989-02-15
DK175538B1 true DK175538B1 (da) 2004-11-29

Family

ID=22196693

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198804489A DK175538B1 (da) 1987-08-14 1988-08-11 Fremgangsmåde til fremstilling af et alfa-amiderende enzym, genmanipuleret mikroorganisme eller værtscelle og rekombinant DNA-molekyle,.............

Country Status (13)

Country Link
US (1) US6319685B1 (ko)
EP (2) EP0529742B1 (ko)
JP (1) JP2979172B2 (ko)
KR (1) KR0136611B1 (ko)
AT (1) ATE198355T1 (ko)
CA (1) CA1341454C (ko)
DE (1) DE3856446T2 (ko)
DK (1) DK175538B1 (ko)
FI (1) FI883722A (ko)
HU (3) HU213227B (ko)
LT (1) LT4029B (ko)
NO (1) NO883592L (ko)
RU (2) RU2080385C1 (ko)

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US4708934A (en) * 1984-09-27 1987-11-24 Unigene Laboratories, Inc. α-amidation enzyme
US6319685B1 (en) 1984-09-27 2001-11-20 Unigene Laboratories, Inc. Alpha-amidating enzyme compositions and processes for their production and use
US5789234A (en) * 1987-08-14 1998-08-04 Unigene Laboratories, Inc. Expression systems for amidating enzyme
US5871995A (en) * 1989-08-15 1999-02-16 Shiseido Company, Ltd. Purified enzymes participating in C-terminal amidation
DE69114583T2 (de) * 1990-06-01 1996-06-13 Japat Ltd Peptdidylhydroxylglycine N-C Lyase und DNS dafür kodierend.
US5580751A (en) * 1990-09-14 1996-12-03 Carlsberg A/S Process for the preparation of C-terminally amidated peptides
DK67691D0 (da) * 1991-03-01 1991-04-15 Carlbiotech Ltd As Enzymatisk fremgangsmaade til c-terminal modificering af peptider og mellemprodukter til brug ved fremgangsmaaden
AU4415796A (en) * 1994-12-07 1996-06-26 Bionebraska, Inc. Production of c-terminal amidated peptides from recombinant protein constructs
US5912014A (en) * 1996-03-15 1999-06-15 Unigene Laboratories, Inc. Oral salmon calcitonin pharmaceutical products
ATE484572T1 (de) 1997-04-16 2010-10-15 Unigene Lab Inc Direkte expression von peptiden ins kulturmedium
DE60331336D1 (de) 2002-07-26 2010-04-01 Ajinomoto Kk Verfahren zur herstellung eines tripeptids oder höheren peptids
US8088734B2 (en) 2003-01-21 2012-01-03 Unigene Laboratories Inc. Oral delivery of peptides
US7648965B2 (en) 2004-05-14 2010-01-19 Unigene Laboratories Inc. Method for fostering bone formation and preservation
US7531518B2 (en) 2004-05-14 2009-05-12 Unigene Laboratories Inc. Method for fostering bone formation and preservation
US20060089723A1 (en) * 2004-10-25 2006-04-27 Murphy Kieran P Method for bone augmentation
US7445911B2 (en) * 2004-11-24 2008-11-04 Unigene Laboratories Inc. Enzymatic reactions in the presence of keto acids
PA8660701A1 (es) 2005-02-04 2006-09-22 Pfizer Prod Inc Agonistas de pyy y sus usos
US20060293667A1 (en) * 2005-05-19 2006-12-28 Agnes Vignery Bone implant device and methods of using same
US8377863B2 (en) 2007-05-29 2013-02-19 Unigene Laboratories Inc. Peptide pharmaceutical for oral delivery
WO2010085700A2 (en) 2009-01-22 2010-07-29 Unigene Laboratories Inc. Treatment for obesity
KR20120116942A (ko) 2009-11-23 2012-10-23 아밀린 파마슈티칼스, 인크. 폴리펩티드 접합체
TW201138808A (en) 2010-05-03 2011-11-16 Bristol Myers Squibb Co Serum albumin binding molecules
EA201390450A1 (ru) 2010-09-28 2013-07-30 Амилин Фармасьютикалс, Ллк. Полипептиды с увеличенной продолжительностью действия
BR112013007388B1 (pt) 2010-09-28 2022-01-04 Amylin Pharmaceuticals, Llc Polipeptídeo tendo domínio de ligação de albumina e leptina, seu uso no tratamento de doenças, bem como composição farmacêutica que o compreende
WO2012174397A2 (en) 2011-06-15 2012-12-20 Unigene Laboratories, Inc. Anti-inflammatory pharmaceutical products
US20140256621A1 (en) 2011-07-08 2014-09-11 Astrazeneca Pharmaceuticals Lp Engineered poypeptides having enhanced duration of action and reduced immunogenicity
JP6006309B2 (ja) 2011-07-08 2016-10-12 アミリン・ファーマシューティカルズ，リミテッド・ライアビリティ・カンパニーＡｍｙｌｉｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ，Ｌｌｃ 作用持続期間が増大し、免疫原性が減少した操作されたポリペプチド
US9533022B2 (en) 2011-11-02 2017-01-03 KeyBioscience A/S Peptide analogs for treating diseases and disorders
CN103998052B (zh) 2011-11-02 2016-08-17 关键生物科学有限公司 用于治疗疾病和紊乱的肽类似物
LT3095484T (lt) 2011-11-02 2018-08-10 Keybioscience Ag Kalcitonino mimetikai, skirti ligų ir sutrikimų gydymui
RU2689551C1 (ru) 2013-11-14 2019-05-28 Кибайосайнс Аг Миметик кальцитонина для лечения болезней и нарушений
EP3077049B1 (en) 2013-12-02 2019-06-19 Stealth BioTherapeutics Corp Compositions for use in treating vitiligo
WO2015183995A2 (en) 2014-05-28 2015-12-03 Stealth Peptides International, Inc. Therapeutic compositions including frataxin, lactoferrin, and mitochondrial energy generating enzymes, and uses thereof
US20170182117A1 (en) 2014-05-28 2017-06-29 Stealth Biotherapeutics Corp Therapeutic compositions including therapeutic small molecules and uses thereof
WO2015195737A1 (en) 2014-06-17 2015-12-23 Stealth Peptides International, Inc. Methods of identifying and monitoring mitochondrial dysfunction using monocyte screening
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CA2973099C (en) 2015-01-12 2021-09-14 Enteris Biopharma, Inc. Solid oral dosage forms
GB201704429D0 (en) 2017-03-21 2017-05-03 Keybioscience Ag Calcitonin mimetics for treating diseases and disorders
GB201707955D0 (en) 2017-05-18 2017-07-05 Keybioscience Ag Dual amylin and calcitonin receptor agonists for treating diseases and disorders
GB201813677D0 (en) 2018-08-22 2018-10-03 Keybioscience Ag Calcitonin mimetics for treating diseases and disorders
GB201813678D0 (en) 2018-08-22 2018-10-03 Keybioscience Ag Acylated calcitonin mimetics
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Also Published As

Publication number Publication date
DK448988A (da) 1989-02-15
KR890003947A (ko) 1989-04-19
HU212071A9 (en) 1996-02-28
DK448988D0 (da) 1988-08-11
AU2057688A (en) 1989-02-16
HU213227B (en) 1997-07-28
FI883722A (fi) 1989-02-15
CA1341454C (en) 2004-04-20
HU00552A9 (en) 1995-10-30
EP0308067A2 (en) 1989-03-22
KR0136611B1 (ko) 1998-04-25
AU629795B2 (en) 1992-10-15
RU2252961C2 (ru) 2005-05-27
DE3856446T2 (de) 2001-07-12
JPH02104281A (ja) 1990-04-17
NO883592L (no) 1989-02-15
LTIP1656A (en) 1995-07-25
DE3856446D1 (de) 2001-02-01
LT4029B (en) 1996-08-26
NO883592D0 (no) 1988-08-12
EP0308067A3 (en) 1989-07-26
JP2979172B2 (ja) 1999-11-15
RU2080385C1 (ru) 1997-05-27
ATE198355T1 (de) 2001-01-15
US6319685B1 (en) 2001-11-20
EP0529742A1 (en) 1993-03-03
EP0529742B1 (en) 2000-12-27
FI883722A0 (fi) 1988-08-10

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