DK175537B1 - Ekspressionsplasmider indeholdende deo-P1-P2-promotoren, og bakterielle værter, der indeholder plasmiderne - Google Patents
Ekspressionsplasmider indeholdende deo-P1-P2-promotoren, og bakterielle værter, der indeholder plasmiderne Download PDFInfo
- Publication number
- DK175537B1 DK175537B1 DK198804549A DK454988A DK175537B1 DK 175537 B1 DK175537 B1 DK 175537B1 DK 198804549 A DK198804549 A DK 198804549A DK 454988 A DK454988 A DK 454988A DK 175537 B1 DK175537 B1 DK 175537B1
- Authority
- DK
- Denmark
- Prior art keywords
- plasmid
- deo
- polypeptide
- analog
- sequence
- Prior art date
Links
- 239000013612 plasmid Substances 0.000 title claims abstract description 488
- 230000001580 bacterial effect Effects 0.000 title claims description 16
- 239000013613 expression plasmid Substances 0.000 title claims description 14
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 183
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 144
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 143
- 229920001184 polypeptide Polymers 0.000 claims abstract description 142
- 230000014509 gene expression Effects 0.000 claims abstract description 109
- 108020004414 DNA Proteins 0.000 claims abstract description 78
- 241000588724 Escherichia coli Species 0.000 claims abstract description 76
- 239000010949 copper Substances 0.000 claims abstract description 48
- 102000019197 Superoxide Dismutase Human genes 0.000 claims abstract description 43
- 108010012715 Superoxide dismutase Proteins 0.000 claims abstract description 43
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 35
- 229910052802 copper Inorganic materials 0.000 claims abstract description 35
- DLINORNFHVEIFE-UHFFFAOYSA-N hydrogen peroxide;zinc Chemical compound [Zn].OO DLINORNFHVEIFE-UHFFFAOYSA-N 0.000 claims abstract description 33
- 108010000521 Human Growth Hormone Proteins 0.000 claims abstract description 23
- 102000002265 Human Growth Hormone Human genes 0.000 claims abstract description 23
- 239000000854 Human Growth Hormone Substances 0.000 claims abstract description 14
- 101710095339 Apolipoprotein E Proteins 0.000 claims abstract description 8
- 102100029470 Apolipoprotein E Human genes 0.000 claims abstract description 8
- 241000283690 Bos taurus Species 0.000 claims abstract description 6
- 102100037362 Fibronectin Human genes 0.000 claims abstract description 5
- 108010067306 Fibronectins Proteins 0.000 claims abstract description 4
- 210000004027 cell Anatomy 0.000 claims description 93
- 108010006025 bovine growth hormone Proteins 0.000 claims description 73
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 69
- 239000002609 medium Substances 0.000 claims description 57
- 239000008103 glucose Substances 0.000 claims description 50
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 49
- 241000588722 Escherichia Species 0.000 claims description 47
- 238000000034 method Methods 0.000 claims description 40
- 238000004519 manufacturing process Methods 0.000 claims description 36
- 150000001413 amino acids Chemical group 0.000 claims description 31
- 229930091371 Fructose Natural products 0.000 claims description 26
- 239000005715 Fructose Substances 0.000 claims description 26
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 26
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 25
- 230000000694 effects Effects 0.000 claims description 19
- 108010051696 Growth Hormone Proteins 0.000 claims description 18
- 102000018997 Growth Hormone Human genes 0.000 claims description 17
- 108020004999 messenger RNA Proteins 0.000 claims description 16
- 108091081024 Start codon Proteins 0.000 claims description 15
- 238000013519 translation Methods 0.000 claims description 15
- 102000053602 DNA Human genes 0.000 claims description 12
- 239000002773 nucleotide Substances 0.000 claims description 12
- 125000003729 nucleotide group Chemical group 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 12
- 239000011701 zinc Substances 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 11
- 230000010076 replication Effects 0.000 claims description 11
- 238000005273 aeration Methods 0.000 claims description 9
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 claims description 8
- 150000002500 ions Chemical class 0.000 claims description 8
- 239000001540 sodium lactate Substances 0.000 claims description 8
- 229940005581 sodium lactate Drugs 0.000 claims description 8
- 235000011088 sodium lactate Nutrition 0.000 claims description 8
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 claims description 8
- OHVGNSMTLSKTGN-BTVCFUMJSA-N [C].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O Chemical compound [C].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O OHVGNSMTLSKTGN-BTVCFUMJSA-N 0.000 claims description 7
- 102000006587 Glutathione peroxidase Human genes 0.000 claims description 6
- 108700016172 Glutathione peroxidases Proteins 0.000 claims description 6
- 108020005091 Replication Origin Proteins 0.000 claims description 6
- 230000004071 biological effect Effects 0.000 claims description 6
- 229910021645 metal ion Inorganic materials 0.000 claims description 6
- 230000008859 change Effects 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 5
- 108091008146 restriction endonucleases Proteins 0.000 claims description 5
- 241001515965 unidentified phage Species 0.000 claims description 5
- WPUMTJGUQUYPIV-JIZZDEOASA-L disodium (S)-malate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](O)CC([O-])=O WPUMTJGUQUYPIV-JIZZDEOASA-L 0.000 claims description 4
- 210000003705 ribosome Anatomy 0.000 claims description 4
- 235000019265 sodium DL-malate Nutrition 0.000 claims description 4
- 239000001394 sodium malate Substances 0.000 claims description 4
- 206010059866 Drug resistance Diseases 0.000 claims description 3
- 239000003623 enhancer Substances 0.000 claims 1
- 230000012010 growth Effects 0.000 abstract description 22
- 230000004927 fusion Effects 0.000 abstract description 16
- 102000009661 Repressor Proteins Human genes 0.000 abstract description 3
- 108010034634 Repressor Proteins Proteins 0.000 abstract description 3
- 239000012634 fragment Substances 0.000 description 144
- 102000004169 proteins and genes Human genes 0.000 description 82
- 235000018102 proteins Nutrition 0.000 description 77
- 238000010276 construction Methods 0.000 description 59
- 238000005520 cutting process Methods 0.000 description 41
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 29
- 229960000723 ampicillin Drugs 0.000 description 28
- 239000013598 vector Substances 0.000 description 27
- 108090000204 Dipeptidase 1 Proteins 0.000 description 20
- 239000004098 Tetracycline Substances 0.000 description 20
- 229960002180 tetracycline Drugs 0.000 description 20
- 229930101283 tetracycline Natural products 0.000 description 20
- 235000019364 tetracycline Nutrition 0.000 description 20
- 150000003522 tetracyclines Chemical class 0.000 description 20
- 230000035897 transcription Effects 0.000 description 20
- 238000013518 transcription Methods 0.000 description 20
- 102000006635 beta-lactamase Human genes 0.000 description 19
- 238000000855 fermentation Methods 0.000 description 18
- 230000004151 fermentation Effects 0.000 description 18
- 239000000499 gel Substances 0.000 description 18
- 108091026890 Coding region Proteins 0.000 description 17
- 229940088598 enzyme Drugs 0.000 description 17
- 238000002474 experimental method Methods 0.000 description 17
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 16
- 102000004190 Enzymes Human genes 0.000 description 16
- 108090000790 Enzymes Proteins 0.000 description 16
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 16
- 229910052799 carbon Inorganic materials 0.000 description 16
- 239000013604 expression vector Substances 0.000 description 15
- 235000001014 amino acid Nutrition 0.000 description 14
- 239000000122 growth hormone Substances 0.000 description 14
- 108020001507 fusion proteins Proteins 0.000 description 13
- 102000037865 fusion proteins Human genes 0.000 description 13
- 101150017120 sod gene Proteins 0.000 description 13
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 11
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 11
- 238000001962 electrophoresis Methods 0.000 description 10
- 230000005030 transcription termination Effects 0.000 description 10
- 230000015572 biosynthetic process Effects 0.000 description 9
- 239000012071 phase Substances 0.000 description 9
- 238000003786 synthesis reaction Methods 0.000 description 9
- 238000012217 deletion Methods 0.000 description 8
- 230000037430 deletion Effects 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 102000004594 DNA Polymerase I Human genes 0.000 description 7
- 108010017826 DNA Polymerase I Proteins 0.000 description 7
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 7
- 239000000284 extract Substances 0.000 description 7
- 238000011049 filling Methods 0.000 description 7
- 238000001502 gel electrophoresis Methods 0.000 description 7
- 230000006698 induction Effects 0.000 description 7
- 229930182817 methionine Natural products 0.000 description 7
- 230000009466 transformation Effects 0.000 description 7
- 102000003960 Ligases Human genes 0.000 description 6
- 108090000364 Ligases Proteins 0.000 description 6
- 239000000020 Nitrocellulose Substances 0.000 description 6
- 239000011543 agarose gel Substances 0.000 description 6
- 238000009396 hybridization Methods 0.000 description 6
- 230000001965 increasing effect Effects 0.000 description 6
- 229920001220 nitrocellulos Polymers 0.000 description 6
- 238000002497 phase alternated rotation of magnetisation Methods 0.000 description 6
- 229920002401 polyacrylamide Polymers 0.000 description 6
- 230000001105 regulatory effect Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 101710154303 Cyclic AMP receptor protein Proteins 0.000 description 5
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 5
- 230000005526 G1 to G0 transition Effects 0.000 description 5
- 102100032891 Superoxide dismutase [Mn], mitochondrial Human genes 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 239000002299 complementary DNA Substances 0.000 description 5
- 238000012258 culturing Methods 0.000 description 5
- 238000005457 optimization Methods 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 230000002103 transcriptional effect Effects 0.000 description 5
- 102100034613 Annexin A2 Human genes 0.000 description 4
- 108090000668 Annexin A2 Proteins 0.000 description 4
- 229910017518 Cu Zn Inorganic materials 0.000 description 4
- 229910017752 Cu-Zn Inorganic materials 0.000 description 4
- 229910017943 Cu—Zn Inorganic materials 0.000 description 4
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 4
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 4
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 4
- 108091034117 Oligonucleotide Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 235000003704 aspartic acid Nutrition 0.000 description 4
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 4
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000000295 complement effect Effects 0.000 description 4
- TVZPLCNGKSPOJA-UHFFFAOYSA-N copper zinc Chemical compound [Cu].[Zn] TVZPLCNGKSPOJA-UHFFFAOYSA-N 0.000 description 4
- 239000000539 dimer Substances 0.000 description 4
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 238000002741 site-directed mutagenesis Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 3
- 108020004705 Codon Proteins 0.000 description 3
- 239000006142 Luria-Bertani Agar Substances 0.000 description 3
- JQECLVNLAZGHRQ-CIUDSAMLSA-N Met-Asp-Gln Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](C(O)=O)CCC(N)=O JQECLVNLAZGHRQ-CIUDSAMLSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 102000009097 Phosphorylases Human genes 0.000 description 3
- 108010073135 Phosphorylases Proteins 0.000 description 3
- 101100173636 Rattus norvegicus Fhl2 gene Proteins 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 102000013537 Thymidine Phosphorylase Human genes 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 238000010217 densitometric analysis Methods 0.000 description 3
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 229960003180 glutathione Drugs 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 229940088597 hormone Drugs 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 3
- 239000007320 rich medium Substances 0.000 description 3
- 238000011218 seed culture Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 108010077805 Bacterial Proteins Proteins 0.000 description 2
- 101000928995 Caenorhabditis elegans Putative deoxyribose-phosphate aldolase Proteins 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 2
- UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 2
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 2
- 241001288713 Escherichia coli MC1061 Species 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 102000048120 Galactokinases Human genes 0.000 description 2
- 108700023157 Galactokinases Proteins 0.000 description 2
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 2
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 2
- 229930010555 Inosine Natural products 0.000 description 2
- 102000003992 Peroxidases Human genes 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 206010035148 Plague Diseases 0.000 description 2
- 101710132082 Pyrimidine/purine nucleoside phosphorylase Proteins 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 101710128967 Somatotropin-1 Proteins 0.000 description 2
- 244000269722 Thea sinensis Species 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 240000004922 Vigna radiata Species 0.000 description 2
- 235000010721 Vigna radiata var radiata Nutrition 0.000 description 2
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 2
- 108091006374 cAMP receptor proteins Proteins 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical group O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 2
- 238000000432 density-gradient centrifugation Methods 0.000 description 2
- 102000030794 deoxyribose-phosphate aldolase Human genes 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 235000019797 dipotassium phosphate Nutrition 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000003111 growth hormone derivative Substances 0.000 description 2
- 229940029575 guanosine Drugs 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 239000000411 inducer Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 229960003786 inosine Drugs 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- NUJOXMJBOLGQSY-UHFFFAOYSA-N manganese dioxide Chemical compound O=[Mn]=O NUJOXMJBOLGQSY-UHFFFAOYSA-N 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 230000012666 negative regulation of transcription by glucose Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- NWXMGUDVXFXRIG-WESIUVDSSA-N (4s,4as,5as,6s,12ar)-4-(dimethylamino)-1,6,10,11,12a-pentahydroxy-6-methyl-3,12-dioxo-4,4a,5,5a-tetrahydrotetracene-2-carboxamide Chemical compound C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(=O)C(C(N)=O)=C(O)[C@@]4(O)C(=O)C3=C(O)C2=C1O NWXMGUDVXFXRIG-WESIUVDSSA-N 0.000 description 1
- 101150110188 30 gene Proteins 0.000 description 1
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 1
- 241000238876 Acari Species 0.000 description 1
- 241001479434 Agfa Species 0.000 description 1
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
- 239000005695 Ammonium acetate Substances 0.000 description 1
- 240000001426 Amorpha canescens Species 0.000 description 1
- 241000272814 Anser sp. Species 0.000 description 1
- 102000008128 Apolipoprotein E3 Human genes 0.000 description 1
- 108010060215 Apolipoprotein E3 Proteins 0.000 description 1
- 102000007592 Apolipoproteins Human genes 0.000 description 1
- 108010071619 Apolipoproteins Proteins 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 1
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 description 1
- 102000018832 Cytochromes Human genes 0.000 description 1
- 108010052832 Cytochromes Proteins 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- KKZFLSZAWCYPOC-VPENINKCSA-N Deoxyribose 5-phosphate Chemical compound O[C@H]1C[C@H](O)[C@@H](COP(O)(O)=O)O1 KKZFLSZAWCYPOC-VPENINKCSA-N 0.000 description 1
- 101710204837 Envelope small membrane protein Proteins 0.000 description 1
- 102400000102 Eosinophil granule major basic protein Human genes 0.000 description 1
- 101900137526 Escherichia coli Galactokinase Proteins 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 210000000712 G cell Anatomy 0.000 description 1
- 101150117524 GPX gene Proteins 0.000 description 1
- 101000771674 Homo sapiens Apolipoprotein E Proteins 0.000 description 1
- 101001027128 Homo sapiens Fibronectin Proteins 0.000 description 1
- 101000868115 Homo sapiens Superoxide dismutase [Mn], mitochondrial Proteins 0.000 description 1
- 101150017040 I gene Proteins 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 229910004616 Na2MoO4.2H2 O Inorganic materials 0.000 description 1
- 101710141454 Nucleoprotein Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 101000702641 Picea abies Superoxide dismutase [Cu-Zn], chloroplastic Proteins 0.000 description 1
- 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 1
- 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 1
- 229940096437 Protein S Drugs 0.000 description 1
- 102000029301 Protein S Human genes 0.000 description 1
- 108010066124 Protein S Proteins 0.000 description 1
- 102000001218 Rec A Recombinases Human genes 0.000 description 1
- 108010055016 Rec A Recombinases Proteins 0.000 description 1
- 101710088839 Replication initiation protein Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- IVOMOUWHDPKRLL-UHFFFAOYSA-N UNPD107823 Natural products O1C2COP(O)(=O)OC2C(O)C1N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-UHFFFAOYSA-N 0.000 description 1
- 101710100170 Unknown protein Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000000956 alloy Substances 0.000 description 1
- 229910045601 alloy Inorganic materials 0.000 description 1
- 229940043376 ammonium acetate Drugs 0.000 description 1
- 235000019257 ammonium acetate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 210000003578 bacterial chromosome Anatomy 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N biotin Natural products N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- LLSDKQJKOVVTOJ-UHFFFAOYSA-L calcium chloride dihydrate Chemical compound O.O.[Cl-].[Cl-].[Ca+2] LLSDKQJKOVVTOJ-UHFFFAOYSA-L 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000006652 catabolic pathway Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000002925 chemical effect Effects 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 229910001431 copper ion Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 229940095074 cyclic amp Drugs 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- MSJMDZAOKORVFC-UAIGNFCESA-L disodium maleate Chemical compound [Na+].[Na+].[O-]C(=O)\C=C/C([O-])=O MSJMDZAOKORVFC-UAIGNFCESA-L 0.000 description 1
- -1 e.g. Proteins 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000009585 enzyme analysis Methods 0.000 description 1
- 108010048134 estramustine-binding protein Proteins 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000003804 extraction from natural source Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 150000002303 glucose derivatives Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 1
- 102000053020 human ApoE Human genes 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000001320 lysogenic effect Effects 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- JSPANIZMKMFECH-UHFFFAOYSA-L manganese(II) sulfate dihydrate Chemical compound O.O.[Mn+2].[O-]S([O-])(=O)=O JSPANIZMKMFECH-UHFFFAOYSA-L 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000036651 mood Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 229940046166 oligodeoxynucleotide Drugs 0.000 description 1
- 238000002515 oligonucleotide synthesis Methods 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 150000008300 phosphoramidites Chemical class 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004584 polyacrylic acid Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 239000013587 production medium Substances 0.000 description 1
- 230000007425 progressive decline Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 210000003689 pubic bone Anatomy 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002213 purine nucleotide Substances 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000007363 regulatory process Effects 0.000 description 1
- HJORMJIFDVBMOB-UHFFFAOYSA-N rolipram Chemical compound COC1=CC=C(C2CC(=O)NC2)C=C1OC1CCCC1 HJORMJIFDVBMOB-UHFFFAOYSA-N 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- BAZAXWOYCMUHIX-UHFFFAOYSA-M sodium perchlorate Chemical compound [Na+].[O-]Cl(=O)(=O)=O BAZAXWOYCMUHIX-UHFFFAOYSA-M 0.000 description 1
- 229910001488 sodium perchlorate Inorganic materials 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 229940032362 superoxide dismutase Drugs 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 108010042582 tosylarginine methyl ester hydrolase Proteins 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 108700026215 vpr Genes Proteins 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/775—Apolipopeptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Endocrinology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Magnetic Resonance Imaging Apparatus (AREA)
- Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US8615987A | 1987-08-14 | 1987-08-14 | |
US8615987 | 1987-08-14 |
Publications (3)
Publication Number | Publication Date |
---|---|
DK454988D0 DK454988D0 (da) | 1988-08-12 |
DK454988A DK454988A (da) | 1989-02-15 |
DK175537B1 true DK175537B1 (da) | 2004-11-29 |
Family
ID=22196678
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK198804549A DK175537B1 (da) | 1987-08-14 | 1988-08-12 | Ekspressionsplasmider indeholdende deo-P1-P2-promotoren, og bakterielle værter, der indeholder plasmiderne |
Country Status (17)
Country | Link |
---|---|
EP (1) | EP0303972B1 (ko) |
JP (1) | JP2752092B2 (ko) |
KR (1) | KR970000594B1 (ko) |
AT (1) | ATE161888T1 (ko) |
AU (1) | AU607249B2 (ko) |
CA (1) | CA1309043C (ko) |
DE (1) | DE3856101T2 (ko) |
DK (1) | DK175537B1 (ko) |
ES (1) | ES2112240T3 (ko) |
FI (1) | FI104429B (ko) |
GR (1) | GR3026136T3 (ko) |
HU (1) | HU213418B (ko) |
IL (1) | IL87409A (ko) |
NO (1) | NO302185B1 (ko) |
NZ (1) | NZ225760A (ko) |
PT (1) | PT88271B (ko) |
ZA (1) | ZA885983B (ko) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE132902T1 (de) * | 1987-03-14 | 1996-01-15 | Boehringer Ingelheim Int | Humane mangan-superoxiddismutase (hmn-sod) |
US5260204A (en) * | 1987-03-14 | 1993-11-09 | Boehringer Ingelheim International Gmbh | Human manganese superoxide dismutase (hMn-SOD) |
US5210180A (en) * | 1989-10-27 | 1993-05-11 | American Cyanamid Company | Enhancement of porcine somatotropin activity |
US6165465A (en) * | 1990-05-15 | 2000-12-26 | American Cyanamid Company | Immunologic enhancement of the somatogenic effect of somatotropin with an antibody |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0753111B2 (ja) * | 1982-09-16 | 1995-06-07 | ベンツォン ファーマ エイ/エス | 条件非制御の複製挙動を持つプラスミド |
-
1988
- 1988-08-09 NZ NZ225760A patent/NZ225760A/xx unknown
- 1988-08-11 DE DE3856101T patent/DE3856101T2/de not_active Expired - Lifetime
- 1988-08-11 AT AT88113071T patent/ATE161888T1/de not_active IP Right Cessation
- 1988-08-11 ES ES88113071T patent/ES2112240T3/es not_active Expired - Lifetime
- 1988-08-11 IL IL87409A patent/IL87409A/en not_active IP Right Cessation
- 1988-08-11 EP EP88113071A patent/EP0303972B1/en not_active Expired - Lifetime
- 1988-08-12 ZA ZA885983A patent/ZA885983B/xx unknown
- 1988-08-12 DK DK198804549A patent/DK175537B1/da not_active IP Right Cessation
- 1988-08-12 NO NO883611A patent/NO302185B1/no not_active IP Right Cessation
- 1988-08-12 HU HU884331A patent/HU213418B/hu unknown
- 1988-08-12 FI FI883749A patent/FI104429B/fi not_active IP Right Cessation
- 1988-08-12 PT PT88271A patent/PT88271B/pt not_active IP Right Cessation
- 1988-08-12 AU AU20982/88A patent/AU607249B2/en not_active Expired
- 1988-08-15 JP JP63203068A patent/JP2752092B2/ja not_active Expired - Lifetime
- 1988-08-15 CA CA000574717A patent/CA1309043C/en not_active Expired - Lifetime
- 1988-08-16 KR KR1019880010394A patent/KR970000594B1/ko not_active IP Right Cessation
-
1998
- 1998-02-12 GR GR980400309T patent/GR3026136T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
DE3856101T2 (de) | 1998-07-23 |
EP0303972A2 (en) | 1989-02-22 |
AU2098288A (en) | 1989-03-09 |
PT88271B (pt) | 1995-07-18 |
FI104429B (fi) | 2000-01-31 |
FI883749A0 (fi) | 1988-08-12 |
HUT48681A (en) | 1989-06-28 |
EP0303972A3 (en) | 1991-12-18 |
GR3026136T3 (en) | 1998-05-29 |
CA1309043C (en) | 1992-10-20 |
KR890003953A (ko) | 1989-04-19 |
JP2752092B2 (ja) | 1998-05-18 |
ZA885983B (en) | 1989-05-30 |
NO302185B1 (no) | 1998-02-02 |
FI883749A (fi) | 1989-02-15 |
EP0303972B1 (en) | 1998-01-07 |
KR970000594B1 (ko) | 1997-01-14 |
NO883611L (no) | 1989-02-15 |
ATE161888T1 (de) | 1998-01-15 |
DK454988A (da) | 1989-02-15 |
NZ225760A (en) | 1991-02-26 |
DE3856101D1 (de) | 1998-02-12 |
AU607249B2 (en) | 1991-02-28 |
DK454988D0 (da) | 1988-08-12 |
IL87409A (en) | 1998-03-10 |
PT88271A (pt) | 1989-06-30 |
ES2112240T3 (es) | 1998-04-01 |
NO883611D0 (no) | 1988-08-12 |
HU213418B (en) | 1997-06-30 |
JPH01157391A (ja) | 1989-06-20 |
IL87409A0 (en) | 1989-01-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DK173186B1 (da) | Gærpromotor, vektor indeholdende en sådan promotor, gær transformeret med en sådan vektor og fremgangsmåde til fremstilling | |
JP2502508B2 (ja) | 調節領域dna断片及びアルコ―ルオキシダ―ゼ指定遺伝子、それらを含有するプラスミド及びその形質転換酵母又は純粋培養物並びに同酵母によるポリペプチドの製造方法 | |
EP0200590B1 (fr) | Procédé de préparation microbiologique de la sérum-albumine humaine | |
Shinagawa et al. | Regulation of the pho regulon in Escherichia coli K-12: genetic and physiological regulation of the positive regulatory gene phoB | |
US7138564B2 (en) | Isolated Amaranthus agglutinin gene and uses thereof | |
US5795776A (en) | Expression plasmids regulated by an OSMB promoter | |
EP0189998A1 (en) | A process for preparing glucagon or derivatives thereof in a transformed yeast strain | |
FI120266B (fi) | Öljyonteloproteiinit arvokkaiden peptidien kantajina kasveissa | |
DK175534B1 (da) | Rekombinant DNA-molekyle, fremgangsmåde til dets fremstilling, transformeret vært, fremgangsmåde til dens fremstilling, fremgangsmåde til fremstilling af et polypeptid samt pectinlyaser | |
AU653922B2 (en) | Enhancement of cell growth by expression of a cloned hemoglobin gene | |
DK175537B1 (da) | Ekspressionsplasmider indeholdende deo-P1-P2-promotoren, og bakterielle værter, der indeholder plasmiderne | |
US5593860A (en) | Expression plasmids containing the deo promoter, and bacterial hosts containing the plasmids | |
JP3330941B2 (ja) | ヘム・タンパク質の製造方法 | |
EP0952158B1 (en) | Modified methylotrophic p. pastoris yeast which secretes human growth hormone | |
CN110894219A (zh) | 毕赤酵母转录因子hac1、蛋白、巴斯德毕赤酵母及其制备和应用 | |
US6008051A (en) | Recombinant vector and process for cell flotation | |
Ladygin et al. | Transformation of Chlamydomonas reinhardtii CW-15 with the hygromycin phosphotransferase gene as a selectable marker | |
JP3709422B2 (ja) | ニトリラーゼ遺伝子発現に関わる調節因子およびその遺伝子 | |
DE112019000467T5 (de) | Rekombinanter Mikroorganismus, Verfahren zu dessen Herstellung und seine Anwendung bei der Herstellung von Coenzym Q10 | |
NO853313L (no) | 3`-ekspresjonsforbedrende fragmenter og metode. | |
JPH04330294A (ja) | ポリペプチドの発現方法 | |
RU2465315C1 (ru) | РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК pPBS-St9, КОДИРУЮЩАЯ ПОЛИПЕПТИД СОМАТОТРОПИНА, И ШТАММ ДРОЖЖЕЙ SACCHAROMYCES CEREVISIAE ДЛЯ ПРОДУКЦИИ РЕКОМБИНАНТНОГО СОМАТОТРОПИНА | |
CN106636190B (zh) | 一种表达蛋白质或多肽的方法及其专用表达盒 | |
Kim et al. | Enhanced expression of high-affinity iron transporters via H-ferritin production in yeast | |
CN107699588B (zh) | 一种制备鲑鱼降钙素的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUP | Patent expired |