DK1675944T3 - Humane corneale endotelceller og fremgangsmåder til opnåelse og dyrkning af celler til corneal celletransplantation - Google Patents

Humane corneale endotelceller og fremgangsmåder til opnåelse og dyrkning af celler til corneal celletransplantation

Info

Publication number
DK1675944T3
DK1675944T3 DK04794329.5T DK04794329T DK1675944T3 DK 1675944 T3 DK1675944 T3 DK 1675944T3 DK 04794329 T DK04794329 T DK 04794329T DK 1675944 T3 DK1675944 T3 DK 1675944T3
Authority
DK
Denmark
Prior art keywords
endothelial cells
corneal
obtaining
methods
cells
Prior art date
Application number
DK04794329.5T
Other languages
English (en)
Inventor
Ge Ming Lui
Original Assignee
Ge Ming Lui
Cellular Bioengineering Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ge Ming Lui, Cellular Bioengineering Inc filed Critical Ge Ming Lui
Application granted granted Critical
Publication of DK1675944T3 publication Critical patent/DK1675944T3/da

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3808—Endothelial cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/16—Materials or treatment for tissue regeneration for reconstruction of eye parts, e.g. intraocular lens, cornea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Transplantation (AREA)
  • Botany (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Dermatology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Ophthalmology & Optometry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Vascular Medicine (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DK04794329.5T 2003-10-10 2004-10-07 Humane corneale endotelceller og fremgangsmåder til opnåelse og dyrkning af celler til corneal celletransplantation DK1675944T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51034403P 2003-10-10 2003-10-10
PCT/US2004/032933 WO2005038015A1 (en) 2003-10-10 2004-10-07 Human corneal endothelial cells and methods of obtaining and culturing cells for corneal cell transplantation

Publications (1)

Publication Number Publication Date
DK1675944T3 true DK1675944T3 (da) 2011-02-28

Family

ID=34465131

Family Applications (1)

Application Number Title Priority Date Filing Date
DK04794329.5T DK1675944T3 (da) 2003-10-10 2004-10-07 Humane corneale endotelceller og fremgangsmåder til opnåelse og dyrkning af celler til corneal celletransplantation

Country Status (11)

Country Link
US (1) US20070275365A1 (da)
EP (1) EP1675944B1 (da)
JP (1) JP2007508015A (da)
KR (1) KR20070053152A (da)
CN (1) CN1898377A (da)
AT (1) ATE489456T1 (da)
AU (1) AU2004282525B2 (da)
CA (1) CA2542133A1 (da)
DE (1) DE602004030273D1 (da)
DK (1) DK1675944T3 (da)
WO (1) WO2005038015A1 (da)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1296479C (zh) * 2005-07-27 2007-01-24 中国海洋大学 一种人角膜内皮细胞系的构建方法
US20090222086A1 (en) * 2005-10-12 2009-09-03 Ge Ming Lui Resorbable Cornea Button
US8409177B1 (en) 2005-10-13 2013-04-02 Shui T. Lai Intrastromal refractive surgery by inducing shape change of the cornea
US7726811B2 (en) 2006-02-14 2010-06-01 Lai Shui T Subjective wavefront refraction using continuously adjustable wave plates of Zernike function
EP2033044B1 (en) 2006-06-15 2014-07-23 Shui T. Lai Method of manufacturing high visual acuity contact lenses
US7959284B2 (en) 2006-07-25 2011-06-14 Lai Shui T Method of making high precision optics having a wavefront profile
FR2927632B1 (fr) * 2008-02-14 2013-07-19 Basf Beauty Care Solutions F Cornee et muqueuse reconstruites.
KR101032335B1 (ko) * 2008-04-23 2011-05-06 중앙대학교 산학협력단 포유동물 정소로부터 생식선 줄기세포를 수득하는 방법
US8628572B2 (en) * 2009-02-26 2014-01-14 Wake Forest University Health Sciences Corneal endothelial scaffolds and methods of use
CN101597592B (zh) * 2009-06-11 2011-12-28 山东省眼科研究所 人角膜内皮细胞培养液及其制备方法和应用
FR2976587B1 (fr) * 2011-06-20 2015-04-03 Basf Beauty Care Solutions F Methode de dosage in vitro par technique immunologique
EP2733201B1 (en) * 2011-07-15 2018-02-21 Osaka University Method for preparing corneal endothelial cell
WO2013040559A1 (en) 2011-09-16 2013-03-21 Wake Forest University Health Sciences Fabrication of gelatin hydrogel sheet for the transplantation of corneal endothelium
ES2716200T3 (es) 2011-12-06 2019-06-11 Astellas Inst For Regenerative Medicine Método de diferenciación dirigida que produce células endoteliales corneales
US20150044178A1 (en) * 2011-12-28 2015-02-12 Kyoto Prefectural Public University Corporation Normalization of culture of corneal endothelial cells
EP2809152B1 (en) 2012-02-01 2019-11-20 Nayacure Therapeutics Ltd Method for inducing immune tolerance to organ transplants
US10017735B2 (en) * 2012-07-18 2018-07-10 Singapore Health Services Pte Ltd Cell culture of corneal endothelial cells
WO2014038866A1 (ko) * 2012-09-05 2014-03-13 아주대학교산학협력단 연골세포 유래 세포외 기질막을 이용한 신생혈관질환 치료용 조성물 및 각막 또는 결막 이식재
US10655102B2 (en) 2013-05-03 2020-05-19 Emmetrope Ophthalmics Llc Identification and isolation of human corneal endothelial cells (HCECS)
MX2016006915A (es) 2013-11-27 2017-01-23 Kyoto Prefectural Public Univ Corp Aplicacion de laminina a cultivo de celulas endoteliales de la cornea.
EP3213761B1 (en) 2014-10-31 2021-05-19 Kyoto Prefectural Public University Corporation Novel treatment of retina using laminin
CN107073067A (zh) * 2014-10-31 2017-08-18 京都府公立大学法人 使用层粘连蛋白的新的角膜的治疗
CN106497863B (zh) * 2015-09-07 2019-10-11 江苏齐氏生物科技有限公司 一种大鼠角膜内皮细胞的分离、纯化及培养方法
CN106591216B (zh) * 2016-12-12 2021-07-27 深圳市眼科医院 一种人正常角膜上皮细胞及其用途
JP6957893B2 (ja) * 2017-02-20 2021-11-02 東洋製罐グループホールディングス株式会社 細胞培養方法、及び細胞培養システム
CN108619568B (zh) * 2018-04-28 2020-11-20 山东省眼科研究所 一种用于细胞移植的迷你植片及其制备方法
US11154052B2 (en) 2018-09-14 2021-10-26 University Of Miami Dual-chamber vial for corneal graft preservation
JP6664755B1 (ja) * 2018-10-02 2020-03-13 学校法人同志社 角膜内皮細胞を保存するための方法および容器
KR20210069640A (ko) 2018-10-02 2021-06-11 학교법인 도시샤 각막 내피 세포를 보존하기 위한 방법 및 용기
CN115197913A (zh) * 2021-04-13 2022-10-18 江苏齐氏生物科技有限公司 一种原代角膜内皮细胞培养液及其应用
BR112023021993A2 (pt) 2021-05-03 2024-02-20 Astellas Inst For Regenerative Medicine Métodos de geração de células endoteliais da córnea madura

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4554051B2 (ja) * 2000-09-08 2010-09-29 Ｈｏｙａ株式会社 角膜の再構築方法
US7041506B2 (en) * 2001-11-19 2006-05-09 Becton Dickinson And Company Peptides promoting cell adherence, growth and secretion

Also Published As

Publication number Publication date
EP1675944B1 (en) 2010-11-24
WO2005038015A1 (en) 2005-04-28
CN1898377A (zh) 2007-01-17
DE602004030273D1 (de) 2011-01-05
ATE489456T1 (de) 2010-12-15
EP1675944A1 (en) 2006-07-05
JP2007508015A (ja) 2007-04-05
US20070275365A1 (en) 2007-11-29
AU2004282525A1 (en) 2005-04-28
KR20070053152A (ko) 2007-05-23
CA2542133A1 (en) 2005-04-28
AU2004282525B2 (en) 2010-09-23

Similar Documents

Publication Publication Date Title
DK1675944T3 (da) Humane corneale endotelceller og fremgangsmåder til opnåelse og dyrkning af celler til corneal celletransplantation
CN101755046B (zh) 采用间充质干细胞制备真皮乳头组织的方法
CA2751985C (en) Method for the cryopreservation of cells, artificial cell constructs or three-dimensional complex tissues assemblies
Hassanein et al. Recellularization via the bile duct supports functional allogenic and xenogenic cell growth on a decellularized rat liver scaffold
ATE493203T1 (de) Verfahren und gerät zur herstellung von genetisch transformierbarem pflanzengewebe
SE0201328D0 (sv) Hematopoietic differentiation of human embryonic stem cells
MXPA02007137A (es) Fuente de tejido hepatico.
DK1483371T3 (da) Isolerings- og kulturdyrkningsmetoder til mesenkymstamceller fra navlestrengsblod og differentieringsmetode til fra-navlestrengsblod-opnåede mesenkymstamceller til forskellige mesenkymvæv
EP1835023A4 (en) PRECIPITATING CELL DERIVED FROM HUMAN HORNHAIL ENTHELIAL CELLS, OR DERIVED CELL ASSOCIATION, METHOD FOR THE MANUFACTURE THEREOF, AND METHOD FOR TRANSPLANTING THE PRECURSOR CELL OR THE CELL ASSOCIATION
CN104662148A (zh) 含有间充质干细胞的条件培养基的角膜内皮细胞培养用培养基
ATE517176T1 (de) Verfahren zur kultivierung von geschmackszellen aus säugern
Spinozzi et al. Evaluation of the suitability of biocompatible carriers as artificial transplants using cultured porcine corneal endothelial cells
DE60120676D1 (de) Verfahren zur entwicklung von epithelialen stammzellen
JP2003532466A5 (da)
Proulx et al. Stem cells of the skin and cornea: their clinical applications in regenerative medicine
CN102697581A (zh) 一种构建组织工程血管的方法
CN101152580A (zh) 用表皮干细胞体外制作组织工程皮肤的方法
Wang et al. Characterization of ex vivo expanded oral mucosal epithelium cells on acellular porcine corneal stroma for ocular surface reconstruction
JP2005261292A (ja) 細胞シートおよびその製造方法
Mariana et al. Hepatocytes isolation from adult rats for liver recellularization
JP2014143971A (ja) シート状細胞培養物の製造方法
Shenvi et al. A rat primary hepatocyte culture model for aging studies
Al Abdulsalam et al. Cultivation of corneal endothelial cells from sheep
CN103468745A (zh) 一种用vegf165基因修饰的毛囊干细胞作为种子细胞的人工皮肤的制备方法
KR20100036773A (ko) 연골세포 조기배양을 위한 연골세포 특이적 배양배지 및 배양방법