DK1664305T3 - Selektionssystem indeholdende non-antibiotisk resistensselektionsmarkør - Google Patents
Selektionssystem indeholdende non-antibiotisk resistensselektionsmarkør Download PDFInfo
- Publication number
- DK1664305T3 DK1664305T3 DK04767054.2T DK04767054T DK1664305T3 DK 1664305 T3 DK1664305 T3 DK 1664305T3 DK 04767054 T DK04767054 T DK 04767054T DK 1664305 T3 DK1664305 T3 DK 1664305T3
- Authority
- DK
- Denmark
- Prior art keywords
- gene
- arad
- vector
- plasmid
- arad gene
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/53—DNA (RNA) vaccination
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (17)
1. Antibiotika-frit selektionssystem omfattende en bakteriecelle der mangler et araD-gen, i hvilket en vektor som bærer et araD-gen er blevet tilføjet som en selektionsmarkør, hvor fra araD-genet af vektoren er udtrykt en aktiv L-ribulose-5-phosphat 4-epimerase.
2. Antibiotika-frit selektionssystem ifølge krav 1, hvor bakteriecellen er en Escherichia coli -celle.
3. Antibiotika-frit selektionssystem ifølge krav 2, hvor en mutation i araD-genet af vektoren introducerer en stopcodon i codon 8 af araD-genet, hvor fra araD-genet af vektoren er udtrykt en aktiv L-ribulose-5-phosphat 4-epimerase.
4. Antibiotika-frit selektionssystem ifølge krav 2, hvor E. coli er en E. coli-stamme JM109.
5. Antibiotika-frit selektionssystem ifølge krav 2 eller 3, hvor E. coli er en E. coli-stamme DH5 alpha-Tl.
6. Antibiotika-frit selektionssystem ifølge krav 2 eller 3, hvor E. coli er en E. coli-stamme AGI.
7. Antibiotika-frit selektionssystem ifølge krav 2, hvor E. co//'-stammen er stamme DH5 alpha-Tl som mangler araD-genet og ulaF-genet.
8. Antibiotika-frit selektionssystem ifølge krav 2, hvor E. co//'-stammen er stamme AGI som mangler araD-genet og sgbE-genet.
9. Antibiotika-frit selektionssystem ifølge et hvilket som helst af kravene 5-6, hvor E. co//'-stammen mangler araD-genet, u/aF-genet, og sgbE-genet.
10. Vektor omfattende et araD-gen med en mutation som genererer en stopcodon i codon 8 af araD-genet som en selektionsmarkør.
11. Vektor ifølge krav 10, hvor vektoren er en ekspressionsvektor omfattende: (a) en DNA-sekvens som koder for et kerne-forankringsprotein operativt forbundet til en heterolog promoter, kerne-forankringsproteinet omfattende (i) et DNA-bindingsdomæne som binder sig til en specifik DNA-sekvens, og (ii) et funktionelt domæne som binder sig til en kernekomponent, eller en funktionel ækvivalent deraf; og (b) en multimeriseret DNA-sekvens som danner et bindingssted til kerneforankringsproteinet, hvor vektoren mangler et papilloma-virus replikationsoprindelsessted.
12. Vektor ifølge krav 11, hvor (a) kerne-forankringsproteinet er E2-proteinet af Bovine Papilloma Virus type 1 (BPV), og (b) den multimeriserede DNA-sekvens er multiple bindingssteder for BPV E2-proteinet inkorporeret i vektoren som en klynge, hvor bindingsstederne kan være som hoved-til-hale strukturer eller kan inkluderes i vektoren med mellemrum anbragt positionering, hvor vektoren mangler et papilloma-virus replikationsoprindelsessted.
13. Vektor ifølge krav 12, yderligere omfattende en deletion i den multimeriserede DNA-sekvens.
14. Vektor ifølge krav 12, yderligere omfattende en mutation i Shine-Dalgarno-sekvensen af araD-genet.
15. Fremgangsmåde til udvælgelse af celler transformeret med et plasmid indeholdende et araD-gen som en selektionsmarkør og interessegenet, fremgangsmåden omfattende at indsætte plasmidet i den araD-deficiente værtscelle og dyrke cellerne i et dyrkningsmedium indeholdende arabinose, hvor fra araD-genet af plasmidet er udtrykt en aktiv L-ribulose-5-phosphat 4-epimerase.
16. Fremgangsmåde ifølge krav 15, hvor cellen er en Escherichia coli -celle.
17. Fremgangsmåde ifølge krav 16, hvor araD-genet af plasmidet muteres genererende en stopcodon i codon 8 af araD-genet, hvor fra araD-genet af plasmidet der udtrykkes en aktiv L-ribulose-5-phosphat 4-epimerase.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI20031319A FI116068B (fi) | 2003-09-15 | 2003-09-15 | Uusi selektiojärjestelmä, siinä käyttökelpoinen vektori, bakteerisolukantoja sekä menetelmä solujen valikoimiseksi |
| PCT/FI2004/000540 WO2005026364A1 (en) | 2003-09-15 | 2004-09-15 | Selection system containing non-antibiotic resistance selection marker |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1664305T3 true DK1664305T3 (da) | 2015-02-09 |
Family
ID=27838984
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK04767054.2T DK1664305T3 (da) | 2003-09-15 | 2004-09-15 | Selektionssystem indeholdende non-antibiotisk resistensselektionsmarkør |
Country Status (22)
| Country | Link |
|---|---|
| US (1) | US7521182B2 (da) |
| EP (2) | EP2949753A1 (da) |
| JP (1) | JP4732348B2 (da) |
| KR (1) | KR101215245B1 (da) |
| CN (1) | CN1882692B (da) |
| AP (1) | AP1993A (da) |
| AU (1) | AU2004272776B2 (da) |
| BR (1) | BRPI0414392A (da) |
| CA (1) | CA2538863C (da) |
| DK (1) | DK1664305T3 (da) |
| EA (1) | EA011823B1 (da) |
| ES (1) | ES2529346T3 (da) |
| FI (1) | FI116068B (da) |
| HK (2) | HK1084975A1 (da) |
| IL (1) | IL173923A0 (da) |
| MX (1) | MXPA06002559A (da) |
| NZ (1) | NZ545937A (da) |
| PL (1) | PL1664305T3 (da) |
| PT (1) | PT1664305E (da) |
| SI (1) | SI1664305T1 (da) |
| WO (1) | WO2005026364A1 (da) |
| ZA (1) | ZA200602153B (da) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2005271247A1 (en) * | 2004-08-13 | 2006-02-16 | The Trustees Of The University Of Pennsylvania | Antibiotic resistance free DNA vaccines |
| EP2942391B1 (en) | 2004-08-13 | 2018-05-23 | The Trustees Of The University Of Pennsylvania | Methods for constructing antibiotic resistance free vaccines |
| ES2642138T3 (es) * | 2011-12-21 | 2017-11-15 | Bionet-Asia Co. Ltd | Cepas de Bordetella pertussis modificadas |
| WO2014018602A1 (en) * | 2012-07-26 | 2014-01-30 | Glycos Biotechnologies, Inc. | Strains and methods for plasmid maintenance |
| WO2014118385A1 (en) | 2013-02-04 | 2014-08-07 | University of Tromsø | The use of dna sequences encoding an interferon as vaccine adjuvants |
| EP3122870B1 (en) | 2014-03-25 | 2022-06-29 | Ginkgo Bioworks Inc. | Methods and genetic systems for cell engineering |
| EP3569710A1 (en) * | 2018-05-16 | 2019-11-20 | BioVersys AG | Bacterial vectors for genetic manipulation of bacteria |
| CN109136247B (zh) * | 2018-08-09 | 2022-03-29 | 天津大学 | 一种用于铅结合蛋白定向进化的双向筛选系统构建方法 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5843760A (en) | 1994-04-15 | 1998-12-01 | Midwest Research Institute | Single zymomonas mobilis strain for xylose and arabinose fermentation |
| RU2370541C2 (ru) | 1994-12-09 | 2009-10-20 | Имерджент Продакт Дивелопмент Юк Лимитед | Vgc2 днк salmonella typhimurium, мутантная бактерия, обладающая пониженной способностью к адаптации к условиям окружающей среды, и способ ее получения |
| KR19990008000A (ko) | 1995-04-24 | 1999-01-25 | 로버트 에스. 화이트 헤드 | 새로운 대사 경로를 만들고 이를 스크리닝하는 방법 |
| FR2738842B1 (fr) | 1995-09-15 | 1997-10-31 | Rhone Poulenc Rorer Sa | Molecule d'adn circulaire a origine de replication conditionnelle, leur procede de preparation et leur utilisation en therapie genique |
| US6479279B2 (en) | 1995-12-29 | 2002-11-12 | Estonian Biocentre | Episomal vectors and uses thereof |
| US6162433A (en) | 1996-04-19 | 2000-12-19 | Medeva Europe Limited | Non antibiotic selectable markers for live vaccines |
| CA2263796A1 (en) | 1996-08-16 | 1998-02-26 | Medical Research Council | Self-replicating episomal expression vectors conferring tissue-specific gene expression |
| US6030807A (en) | 1996-09-10 | 2000-02-29 | The Rockefeller University | Highly regulable promoter for heterologous gene expression |
| KR100230578B1 (ko) | 1997-07-25 | 1999-12-01 | 허영섭 | 포스포리불로키나제를 융합파트너로 이용하는 재조합 인간 부갑상선호르몬의 발현벡터 |
| US6291245B1 (en) * | 1998-07-15 | 2001-09-18 | Roche Diagnostics Gmbh | Host-vector system |
| US6368793B1 (en) | 1998-10-14 | 2002-04-09 | Microgenomics, Inc. | Metabolic selection methods |
| ATE377649T1 (de) | 2000-03-24 | 2007-11-15 | Xoma Technology Ltd | Verfahren und zelle zur expression von rekombinanten proteinprodukten |
| JP5016779B2 (ja) | 2001-05-03 | 2012-09-05 | フィット バイオテク オーワイ | 発現ベクターおよびその使用方法 |
-
2003
- 2003-09-15 FI FI20031319A patent/FI116068B/fi not_active IP Right Cessation
-
2004
- 2004-09-15 ES ES04767054.2T patent/ES2529346T3/es not_active Expired - Lifetime
- 2004-09-15 PL PL04767054T patent/PL1664305T3/pl unknown
- 2004-09-15 PT PT47670542T patent/PT1664305E/pt unknown
- 2004-09-15 NZ NZ545937A patent/NZ545937A/xx not_active IP Right Cessation
- 2004-09-15 MX MXPA06002559A patent/MXPA06002559A/es active IP Right Grant
- 2004-09-15 AP AP2006003577A patent/AP1993A/xx active
- 2004-09-15 BR BRPI0414392-2A patent/BRPI0414392A/pt not_active Application Discontinuation
- 2004-09-15 SI SI200432219T patent/SI1664305T1/sl unknown
- 2004-09-15 WO PCT/FI2004/000540 patent/WO2005026364A1/en active Application Filing
- 2004-09-15 EP EP14191756.7A patent/EP2949753A1/en not_active Withdrawn
- 2004-09-15 CN CN2004800336943A patent/CN1882692B/zh not_active Expired - Fee Related
- 2004-09-15 JP JP2006526650A patent/JP4732348B2/ja not_active Expired - Fee Related
- 2004-09-15 AU AU2004272776A patent/AU2004272776B2/en not_active Ceased
- 2004-09-15 EA EA200600585A patent/EA011823B1/ru not_active IP Right Cessation
- 2004-09-15 DK DK04767054.2T patent/DK1664305T3/da active
- 2004-09-15 US US10/531,870 patent/US7521182B2/en not_active Expired - Fee Related
- 2004-09-15 CA CA2538863A patent/CA2538863C/en not_active Expired - Fee Related
- 2004-09-15 EP EP04767054.2A patent/EP1664305B1/en not_active Expired - Lifetime
-
2006
- 2006-02-23 IL IL173923A patent/IL173923A0/en unknown
- 2006-03-14 KR KR1020067005158A patent/KR101215245B1/ko active IP Right Grant
- 2006-03-14 ZA ZA200602153A patent/ZA200602153B/en unknown
- 2006-06-27 HK HK06107273.7A patent/HK1084975A1/xx not_active IP Right Cessation
-
2016
- 2016-05-05 HK HK16105149.1A patent/HK1217111A1/zh unknown
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