EA200600585A1 - Система селекции, содержащая маркеры селекции, не связанные с устойчивостью к антибиотикам - Google Patents

Система селекции, содержащая маркеры селекции, не связанные с устойчивостью к антибиотикам

Info

Publication number
EA200600585A1
EA200600585A1 EA200600585A EA200600585A EA200600585A1 EA 200600585 A1 EA200600585 A1 EA 200600585A1 EA 200600585 A EA200600585 A EA 200600585A EA 200600585 A EA200600585 A EA 200600585A EA 200600585 A1 EA200600585 A1 EA 200600585A1
Authority
EA
Eurasian Patent Office
Prior art keywords
arad gene
arad
breeding
gene
selection
Prior art date
Application number
EA200600585A
Other languages
English (en)
Other versions
EA011823B1 (ru
Inventor
Танел Тенсон
Силья Лахт
Маарья Адоян
Андрес Мянник
Урве Тоотс
Март Устав
Original Assignee
Фит Байотек Ойй Плс
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Фит Байотек Ойй Плс filed Critical Фит Байотек Ойй Плс
Publication of EA200600585A1 publication Critical patent/EA200600585A1/ru
Publication of EA011823B1 publication Critical patent/EA011823B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/53—DNA (RNA) vaccination

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Система селекции, лишенная генов устойчивости к антибиотикам, которая основана на применении гена araD в качестве маркера селекции, который несет вектор, встроенный в бактериальный штамм, дефицитный по гену araD. Ген araD из E.coli кодирует L-рибулозо-5-фосфат-4-эпимеразу. Способ селекции клеток, трансформированных плазмидой, которая содержит ген araD. Не связанный с антибиотиками маркер селекции делает данную систему подходящей для продукции терапевтических средств. Ген araD не существенен для роста хозяев, но манипуляция с ним влияет на рост в некоторых селективных условиях. Делеция araD ведет к накоплению вещества, токсичного для хозяина, но не для людей. Ген araD относительно мал, и поэтому может конструироваться малая плазмида, которая требует меньше энергии для репликации, и приводит к повышенным скорости роста и выходу.
EA200600585A 2003-09-15 2004-09-15 Бактериальная система селекции и ее применение EA011823B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20031319A FI116068B (fi) 2003-09-15 2003-09-15 Uusi selektiojärjestelmä, siinä käyttökelpoinen vektori, bakteerisolukantoja sekä menetelmä solujen valikoimiseksi
PCT/FI2004/000540 WO2005026364A1 (en) 2003-09-15 2004-09-15 Selection system containing non-antibiotic resistance selection marker

Publications (2)

Publication Number Publication Date
EA200600585A1 true EA200600585A1 (ru) 2006-08-25
EA011823B1 EA011823B1 (ru) 2009-06-30

Family

ID=27838984

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200600585A EA011823B1 (ru) 2003-09-15 2004-09-15 Бактериальная система селекции и ее применение

Country Status (22)

Country Link
US (1) US7521182B2 (ru)
EP (2) EP1664305B1 (ru)
JP (1) JP4732348B2 (ru)
KR (1) KR101215245B1 (ru)
CN (1) CN1882692B (ru)
AP (1) AP1993A (ru)
AU (1) AU2004272776B2 (ru)
BR (1) BRPI0414392A (ru)
CA (1) CA2538863C (ru)
DK (1) DK1664305T3 (ru)
EA (1) EA011823B1 (ru)
ES (1) ES2529346T3 (ru)
FI (1) FI116068B (ru)
HK (2) HK1084975A1 (ru)
IL (1) IL173923A0 (ru)
MX (1) MXPA06002559A (ru)
NZ (1) NZ545937A (ru)
PL (1) PL1664305T3 (ru)
PT (1) PT1664305E (ru)
SI (1) SI1664305T1 (ru)
WO (1) WO2005026364A1 (ru)
ZA (1) ZA200602153B (ru)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8906664B2 (en) 2004-08-13 2014-12-09 The Trustees Of The University Of Pennsylvania Methods for constructing antibiotic resistance free vaccines
AU2005271247A1 (en) * 2004-08-13 2006-02-16 The Trustees Of The University Of Pennsylvania Antibiotic resistance free DNA vaccines
JP5966018B2 (ja) * 2011-12-21 2016-08-10 バイオネット−エイジア，カンパニー・リミテッド 改変ボルデテラ・パータシス株
WO2014018602A1 (en) * 2012-07-26 2014-01-30 Glycos Biotechnologies, Inc. Strains and methods for plasmid maintenance
EP2950816B1 (en) 2013-02-04 2019-07-31 Schweitzer Biotech Company Ltd. The use of dna sequences encoding an interferon as vaccine adjuvants
US20170173086A1 (en) 2014-03-25 2017-06-22 Ginkgo Bioworks, Inc. Methods and Genetic Systems for Cell Engineering
EP3569710A1 (en) * 2018-05-16 2019-11-20 BioVersys AG Bacterial vectors for genetic manipulation of bacteria
CN109136247B (zh) * 2018-08-09 2022-03-29 天津大学 一种用于铅结合蛋白定向进化的双向筛选系统构建方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843760A (en) 1994-04-15 1998-12-01 Midwest Research Institute Single zymomonas mobilis strain for xylose and arabinose fermentation
KR20060099543A (ko) 1994-12-09 2006-09-19 임페리얼 컬리지 이노베이션스 리미티드 유전자의 동정
KR19990008000A (ko) 1995-04-24 1999-01-25 로버트 에스. 화이트 헤드 새로운 대사 경로를 만들고 이를 스크리닝하는 방법
FR2738842B1 (fr) 1995-09-15 1997-10-31 Rhone Poulenc Rorer Sa Molecule d'adn circulaire a origine de replication conditionnelle, leur procede de preparation et leur utilisation en therapie genique
US6479279B2 (en) 1995-12-29 2002-11-12 Estonian Biocentre Episomal vectors and uses thereof
US6162433A (en) 1996-04-19 2000-12-19 Medeva Europe Limited Non antibiotic selectable markers for live vaccines
EP0918874B1 (en) 1996-08-16 2001-03-07 Medical Research Council Self-replicating episomal expression vectors conferring tissue-specific gene expression
WO1998011231A1 (en) 1996-09-10 1998-03-19 The Rockefeller University Highly regulable promoter for heterologous gene expression
KR100230578B1 (ko) 1997-07-25 1999-12-01 허영섭 포스포리불로키나제를 융합파트너로 이용하는 재조합 인간 부갑상선호르몬의 발현벡터
US6291245B1 (en) * 1998-07-15 2001-09-18 Roche Diagnostics Gmbh Host-vector system
US6368793B1 (en) 1998-10-14 2002-04-09 Microgenomics, Inc. Metabolic selection methods
ES2295151T3 (es) 2000-03-24 2008-04-16 Xoma Technology Ltd. Metodos y celulas mejorados para la expresion de productos proteicos recombinantes.
PL206097B1 (pl) * 2001-05-03 2010-06-30 Fit Biotech Oyj Plc Wektor ekspresyjny, jego zastosowanie, sposób wytwarzania tego wektora, komórka gospodarza, kompozycja farmaceutyczna, szczepionka DNA, genowy czynnik terapeutyczny oraz sposób wytwarzania szczepionki DNA

Also Published As

Publication number Publication date
EA011823B1 (ru) 2009-06-30
SI1664305T1 (sl) 2015-04-30
PT1664305E (pt) 2015-02-10
ES2529346T3 (es) 2015-02-19
KR101215245B1 (ko) 2012-12-24
AP1993A (en) 2009-04-09
EP1664305A1 (en) 2006-06-07
US7521182B2 (en) 2009-04-21
FI116068B (fi) 2005-09-15
PL1664305T3 (pl) 2015-04-30
AU2004272776A1 (en) 2005-03-24
HK1217111A1 (zh) 2016-12-23
AP2006003577A0 (en) 2006-04-30
FI20031319A (fi) 2005-03-16
BRPI0414392A (pt) 2006-11-21
DK1664305T3 (en) 2015-02-09
KR20060080194A (ko) 2006-07-07
EP2949753A1 (en) 2015-12-02
MXPA06002559A (es) 2006-06-20
US20070036822A1 (en) 2007-02-15
HK1084975A1 (en) 2006-08-11
NZ545937A (en) 2008-08-29
CN1882692A (zh) 2006-12-20
JP4732348B2 (ja) 2011-07-27
IL173923A0 (en) 2006-07-05
CA2538863A1 (en) 2005-03-24
AU2004272776B2 (en) 2008-11-06
FI20031319A0 (fi) 2003-09-15
ZA200602153B (en) 2007-07-25
JP2007505616A (ja) 2007-03-15
CN1882692B (zh) 2012-09-05
WO2005026364A1 (en) 2005-03-24
EP1664305B1 (en) 2014-11-05
CA2538863C (en) 2012-05-29
WO2005026364B1 (en) 2005-05-12

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

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