ATE377649T1 - Verfahren und zelle zur expression von rekombinanten proteinprodukten - Google Patents

Verfahren und zelle zur expression von rekombinanten proteinprodukten

Info

Publication number
ATE377649T1
ATE377649T1 AT01922467T AT01922467T ATE377649T1 AT E377649 T1 ATE377649 T1 AT E377649T1 AT 01922467 T AT01922467 T AT 01922467T AT 01922467 T AT01922467 T AT 01922467T AT E377649 T1 ATE377649 T1 AT E377649T1
Authority
AT
Austria
Prior art keywords
promoter
recombinant protein
protein products
inducible promoter
cell
Prior art date
Application number
AT01922467T
Other languages
English (en)
Inventor
Marc Better
Original Assignee
Xoma Technology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xoma Technology Ltd filed Critical Xoma Technology Ltd
Application granted granted Critical
Publication of ATE377649T1 publication Critical patent/ATE377649T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT01922467T 2000-03-24 2001-03-19 Verfahren und zelle zur expression von rekombinanten proteinprodukten ATE377649T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19212900P 2000-03-24 2000-03-24
US19223800P 2000-03-27 2000-03-27

Publications (1)

Publication Number Publication Date
ATE377649T1 true ATE377649T1 (de) 2007-11-15

Family

ID=26887762

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01922467T ATE377649T1 (de) 2000-03-24 2001-03-19 Verfahren und zelle zur expression von rekombinanten proteinprodukten

Country Status (12)

Country Link
US (2) US6803210B2 (de)
EP (1) EP1268823B1 (de)
JP (1) JP2003528616A (de)
AT (1) ATE377649T1 (de)
AU (2) AU2001249265B2 (de)
CA (1) CA2404046C (de)
CY (1) CY1107173T1 (de)
DE (1) DE60131261T2 (de)
DK (1) DK1268823T3 (de)
ES (1) ES2295151T3 (de)
PT (1) PT1268823E (de)
WO (1) WO2001073082A2 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7754211B2 (en) * 1992-04-10 2010-07-13 Research Development Foundation Immunotoxins directed against c-erbB-2(HER-2/neu) related surface antigens
US8895310B2 (en) 2002-12-02 2014-11-25 Basf Se L-rhamnose-inducible expression systems
FI116068B (fi) * 2003-09-15 2005-09-15 Fit Biotech Oyj Plc Uusi selektiojärjestelmä, siinä käyttökelpoinen vektori, bakteerisolukantoja sekä menetelmä solujen valikoimiseksi
GB0325494D0 (en) * 2003-10-31 2003-12-03 Sbl Vaccin Ab Expression system
WO2006030325A2 (en) * 2004-09-14 2006-03-23 Institut Pasteur Method for identifying antimicrobial molecules which interfere with apoliporotein n-acyltransferase activity
US7846712B2 (en) * 2006-06-01 2010-12-07 Alliance For Sustainable Energy, Llc L-arabinose fermenting yeast
WO2007143247A2 (en) * 2006-06-02 2007-12-13 Midwest Research Institute Cloning and characterization of l-arabinose transporters from non-conventional yeast
WO2010058418A2 (en) * 2008-11-18 2010-05-27 Usv Limited Novel synthetic expression vehicle
WO2012117963A1 (ja) * 2011-02-28 2012-09-07 協和発酵キリン株式会社 有用タンパク質の発現を厳密に制御できる微生物
US20180282405A1 (en) * 2012-08-05 2018-10-04 Absci, Llc Cytoplasmic expression system
ES2621320T3 (es) 2012-08-05 2017-07-03 Absci, Llc Sistema de coexpresión inducible
SG10202107750TA (en) 2017-01-30 2021-09-29 Precigen Inc Molecular switches
KR20220031670A (ko) * 2019-08-05 2022-03-11 와커 헤미 아게 발효 방법에서 재조합 단백질 방출용 박테리아 균주

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61501186A (ja) 1983-10-11 1986-06-19 インターナシヨナル　ジネテイツク　エンジニアリング　インコーポレイテツド タウマチン（Ｔｈａｕｍａｔｉｎ）遺伝子の作製と発現
US5028530A (en) 1985-01-28 1991-07-02 Xoma Corporation AraB promoters and method of producing polypeptides, including cecropins, by microbiological techniques

Also Published As

Publication number Publication date
DE60131261T2 (de) 2008-09-04
AU4926501A (en) 2001-10-08
CA2404046A1 (en) 2001-10-04
CY1107173T1 (el) 2012-10-24
WO2001073082A3 (en) 2002-02-14
ES2295151T3 (es) 2008-04-16
AU2001249265B2 (en) 2006-10-26
US20050260705A1 (en) 2005-11-24
JP2003528616A (ja) 2003-09-30
DK1268823T3 (da) 2008-03-10
CA2404046C (en) 2008-06-03
EP1268823A2 (de) 2003-01-02
DE60131261D1 (de) 2007-12-20
PT1268823E (pt) 2008-02-01
US20020037552A1 (en) 2002-03-28
EP1268823B1 (de) 2007-11-07
US6803210B2 (en) 2004-10-12
WO2001073082A2 (en) 2001-10-04

Similar Documents

Publication Publication Date Title
CY1107173T1 (el) Μεθοδοι και κυτταρα για την εκφραση ανασυνδυασμενων πρωτεϊνικων προϊοντων
DK0733115T3 (da) Hidtil ukendte cellulase-enzymer og systemer til ekspression af disse
ATE317904T1 (de) Überexpression von phytase in hefesystemen
TR199901179T2 (xx) Lepidopterana kar�� aktif olan delta-endotoksinleri eksprese eden transjenik bitkiler.
DK0618976T3 (da) Syntetisk DNA-sekvens med forbedret insekticid virkning i majs
ATE389666T1 (de) Hohe expressionsrate von proteinen
ATE335078T1 (de) Überexpression von säugetier- und virusproteinen
ATE320500T1 (de) Nukleotid-sequenzen zur kontrolle der exprimierung von dns-sequenzen in einem zellulärem wirt
DK1265914T3 (da) WNT-1-relaterede polypeptider og nukleinsyrer, der koder for samme
EP1282722A4 (de) Stabilisierte proteine
WO2003060143A3 (en) Efficient protein expression system
MX2022008727A (es) Factor de crecimiento derivado de mieloide para su uso en el tratamiento o la prevencion de fibrosis, hipertrofia o insuficiencia cardiaca.
BR0111265A (pt) Bacteriocina antilisteria
ATE320485T1 (de) Mutierte furin polypeptide, welche verbesserte eigenschaften aufweisen
DE69828874D1 (de) Zusammensetzungen und verfahren zur eliminierung unerwünschter zellen
WO2000012741A3 (fr) Systeme d'expression inductible
DE59611241D1 (de) Exportsysteme für rekombinante proteine
ATE256747T1 (de) Glykosyltransferase expression in aspergillus
ATE254663T1 (de) Erhöhte produktion von sekretierten proteinen durch rekombinante eukaryotische zellen
EP1889917A3 (de) Verbesserte Verfahren und Zellen zur Expression von rekombinanten Proteinprodukten
FR2696191B1 (fr) Acide nucléique codant pour une alpha-acétolactate décarboxylase et ses applications.
WO2003016490A3 (en) Rna silencing suppression
NZ314777A (en) Vectors containing a gene for an antisigma polypeptide with a multiple cloning site

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1268823

Country of ref document: EP