DE60131261D1 - Verfahren und zelle zur expression von rekombinanten proteinprodukten - Google Patents

Verfahren und zelle zur expression von rekombinanten proteinprodukten

Info

Publication number
DE60131261D1
DE60131261D1 DE60131261T DE60131261T DE60131261D1 DE 60131261 D1 DE60131261 D1 DE 60131261D1 DE 60131261 T DE60131261 T DE 60131261T DE 60131261 T DE60131261 T DE 60131261T DE 60131261 D1 DE60131261 D1 DE 60131261D1
Authority
DE
Germany
Prior art keywords
promoter
recombinant protein
protein products
inducible promoter
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60131261T
Other languages
English (en)
Other versions
DE60131261T2 (de
Inventor
Marc D Better
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xoma Technology Ltd USA
Original Assignee
Xoma Technology Ltd USA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xoma Technology Ltd USA filed Critical Xoma Technology Ltd USA
Application granted granted Critical
Publication of DE60131261D1 publication Critical patent/DE60131261D1/de
Publication of DE60131261T2 publication Critical patent/DE60131261T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60131261T 2000-03-24 2001-03-19 Verbesserte verfahren und zellen zur expression von rekombinanter proteinprodukte Expired - Lifetime DE60131261T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US19212900P 2000-03-24 2000-03-24
US192129P 2000-03-24
US19223800P 2000-03-27 2000-03-27
US192238P 2000-03-27
PCT/US2001/008754 WO2001073082A2 (en) 2000-03-24 2001-03-19 Methods and cells for expression of recombinant protein products

Publications (2)

Publication Number Publication Date
DE60131261D1 true DE60131261D1 (de) 2007-12-20
DE60131261T2 DE60131261T2 (de) 2008-09-04

Family

ID=26887762

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60131261T Expired - Lifetime DE60131261T2 (de) 2000-03-24 2001-03-19 Verbesserte verfahren und zellen zur expression von rekombinanter proteinprodukte

Country Status (12)

Country Link
US (2) US6803210B2 (de)
EP (1) EP1268823B1 (de)
JP (1) JP2003528616A (de)
AT (1) ATE377649T1 (de)
AU (2) AU2001249265B2 (de)
CA (1) CA2404046C (de)
CY (1) CY1107173T1 (de)
DE (1) DE60131261T2 (de)
DK (1) DK1268823T3 (de)
ES (1) ES2295151T3 (de)
PT (1) PT1268823E (de)
WO (1) WO2001073082A2 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7754211B2 (en) * 1992-04-10 2010-07-13 Research Development Foundation Immunotoxins directed against c-erbB-2(HER-2/neu) related surface antigens
ES2288630T3 (es) 2002-12-02 2008-01-16 Basf Aktiengesellschaft Sistema de expresion, que puede ser inducido por medio de la l-ramnosa.
FI116068B (fi) * 2003-09-15 2005-09-15 Fit Biotech Oyj Plc Uusi selektiojärjestelmä, siinä käyttökelpoinen vektori, bakteerisolukantoja sekä menetelmä solujen valikoimiseksi
GB0325494D0 (en) * 2003-10-31 2003-12-03 Sbl Vaccin Ab Expression system
US20060078952A1 (en) * 2004-09-14 2006-04-13 Carine Robichon Method for identifying antimicrobial molecules which interfere with apolipoprotein N-acyltransferase activity
US7846712B2 (en) 2006-06-01 2010-12-07 Alliance For Sustainable Energy, Llc L-arabinose fermenting yeast
WO2007143247A2 (en) * 2006-06-02 2007-12-13 Midwest Research Institute Cloning and characterization of l-arabinose transporters from non-conventional yeast
WO2010058418A2 (en) * 2008-11-18 2010-05-27 Usv Limited Novel synthetic expression vehicle
WO2012117963A1 (ja) * 2011-02-28 2012-09-07 協和発酵キリン株式会社 有用タンパク質の発現を厳密に制御できる微生物
AU2013299910B2 (en) 2012-08-05 2019-01-24 Absci Corporation Inducible coexpression system
US20180282405A1 (en) * 2012-08-05 2018-10-04 Absci, Llc Cytoplasmic expression system
BR112019015754A2 (pt) 2017-01-30 2020-06-09 Intrexon Corp métodos e micro-organismos para fabricar 2,3-butanodiol e derivados deste a partir de carbonos c1
US20240327849A1 (en) * 2019-08-05 2024-10-03 Wacker Chemie Ag Bacterial strain for releasing a recombinant protein in a fermentation method

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985001746A1 (en) 1983-10-11 1985-04-25 Beatrice Companies, Inc. The manufacture and expression of genes for thaumatin
US5028530A (en) 1985-01-28 1991-07-02 Xoma Corporation AraB promoters and method of producing polypeptides, including cecropins, by microbiological techniques

Also Published As

Publication number Publication date
US6803210B2 (en) 2004-10-12
DE60131261T2 (de) 2008-09-04
EP1268823A2 (de) 2003-01-02
US20050260705A1 (en) 2005-11-24
WO2001073082A2 (en) 2001-10-04
CA2404046C (en) 2008-06-03
ATE377649T1 (de) 2007-11-15
JP2003528616A (ja) 2003-09-30
WO2001073082A3 (en) 2002-02-14
US20020037552A1 (en) 2002-03-28
AU2001249265B2 (en) 2006-10-26
AU4926501A (en) 2001-10-08
CY1107173T1 (el) 2012-10-24
ES2295151T3 (es) 2008-04-16
CA2404046A1 (en) 2001-10-04
EP1268823B1 (de) 2007-11-07
DK1268823T3 (da) 2008-03-10
PT1268823E (pt) 2008-02-01

Similar Documents

Publication Publication Date Title
CY1107173T1 (el) Μεθοδοι και κυτταρα για την εκφραση ανασυνδυασμενων πρωτεϊνικων προϊοντων
DK0684770T3 (da) Enzymfoderadditiv og dyrefoder indeholdende det
ATE317904T1 (de) Überexpression von phytase in hefesystemen
TR199901179T2 (xx) Lepidopterana kar�� aktif olan delta-endotoksinleri eksprese eden transjenik bitkiler.
DE69633825D1 (de) Modifiziertes enzym mit lipolytischer aktivität
DE69738586D1 (de) Hohe expressionsrate von proteinen
DE69232725D1 (de) Synthetische dna-sequenz mit verstärkter isektizider aktivität in mais
DE69535147D1 (de) Überexpression von säugetier- und virusproteinen
WO1999055868A3 (en) Fizz proteins
DE69434663D1 (de) Nukleotid-sequenzen zur kontrolle der exprimierung von dns-sequenzen in einem zellulärem wirt
ATE25197T1 (de) Hybridproteinekodierende fusionierte gene, sie enthaltende klonierungsvektoren und deren verwendung.
HUP0001650A2 (hu) Javított expressziós vektorok
DK1265914T3 (da) WNT-1-relaterede polypeptider og nukleinsyrer, der koder for samme
CA2205237A1 (en) Pesticidal composition comprising cryif chimeric and cryia(c) chimeric bacillus thuringiensis delta-endotoxin
EP1282722A4 (de) Stabilisierte proteine
WO2003060143A3 (en) Efficient protein expression system
NZ503316A (en) Polypeptide and nucleic sequences exported from mycobacteria, vectors comprising same and uses for diagnosing and preventing tuberculosis
BR0111265A (pt) Bacteriocina antilisteria
WO2005065348A3 (en) Transactivation system for mammalian cells
ATE320485T1 (de) Mutierte furin polypeptide, welche verbesserte eigenschaften aufweisen
ATE288488T1 (de) Zusammensetzungen und verfahren zur eliminierung unerwünschter zellen
ATE256747T1 (de) Glykosyltransferase expression in aspergillus
ATE254663T1 (de) Erhöhte produktion von sekretierten proteinen durch rekombinante eukaryotische zellen
EP1889917A3 (de) Verbesserte Verfahren und Zellen zur Expression von rekombinanten Proteinprodukten
FR2696191B1 (fr) Acide nucléique codant pour une alpha-acétolactate décarboxylase et ses applications.

Legal Events

Date Code Title Description
8364 No opposition during term of opposition