DK1440157T3 - Methods and means for producing proteins with predetermined post-translational modifications - Google Patents

Methods and means for producing proteins with predetermined post-translational modifications

Info

Publication number
DK1440157T3
DK1440157T3 DK02770322.2T DK02770322T DK1440157T3 DK 1440157 T3 DK1440157 T3 DK 1440157T3 DK 02770322 T DK02770322 T DK 02770322T DK 1440157 T3 DK1440157 T3 DK 1440157T3
Authority
DK
Denmark
Prior art keywords
translational modifications
post
mammalian cells
predetermined post
present
Prior art date
Application number
DK02770322.2T
Other languages
English (en)
Inventor
Johan Christiaan Kapteyn
Dirk Jan Elbertus Opstelten
Petrus Christianus Johannes Josephus Passier
Ronald Hendrik Peter Brus
Abraham Bout
Original Assignee
Crucell Holland Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/NL2001/000792 external-priority patent/WO2003050286A1/en
Priority claimed from PCT/NL2002/000257 external-priority patent/WO2003089468A1/en
Application filed by Crucell Holland Bv filed Critical Crucell Holland Bv
Application granted granted Critical
Publication of DK1440157T3 publication Critical patent/DK1440157T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/02—Drugs for disorders of the nervous system for peripheral neuropathies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
DK02770322.2T 2001-10-29 2002-10-29 Methods and means for producing proteins with predetermined post-translational modifications DK1440157T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PCT/NL2001/000792 WO2003050286A1 (en) 2001-10-29 2001-10-29 Methods and means for producing proteins with predetermined post-translational modifications
PCT/NL2002/000257 WO2003089468A1 (en) 2002-04-19 2002-04-19 Methods and means for producing proteins with predetermined post-translational modifications
PCT/NL2002/000686 WO2003038100A1 (en) 2001-10-29 2002-10-29 Methods and means for producing proteins with predetermined post-translational modifications

Publications (1)

Publication Number Publication Date
DK1440157T3 true DK1440157T3 (da) 2012-05-07

Family

ID=26642018

Family Applications (1)

Application Number Title Priority Date Filing Date
DK02770322.2T DK1440157T3 (da) 2001-10-29 2002-10-29 Methods and means for producing proteins with predetermined post-translational modifications

Country Status (14)

Country Link
US (3) US7304031B2 (da)
EP (2) EP2292770B1 (da)
JP (1) JP4583029B2 (da)
CN (1) CN100347306C (da)
AT (1) ATE542904T1 (da)
AU (1) AU2002335585B2 (da)
BR (1) BR0213402A (da)
CA (2) CA2465007C (da)
DK (1) DK1440157T3 (da)
EA (2) EA008220B1 (da)
ES (1) ES2381104T3 (da)
IL (3) IL161674A (da)
NO (1) NO20042209L (da)
WO (1) WO2003038100A1 (da)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE278794T1 (de) 1995-06-15 2004-10-15 Crucell Holland Bv Verpackungssysteme für humane, menschliche adenoviren, zur verwendung in die gentherapie
US8236561B2 (en) 1999-04-15 2012-08-07 Crucell Holland B.V. Efficient production of IgA in recombinant mammalian cells
US7604960B2 (en) 1999-04-15 2009-10-20 Crucell Holland B.V. Transient protein expression methods
US6855544B1 (en) 1999-04-15 2005-02-15 Crucell Holland B.V. Recombinant protein production in a human cell
WO2003048348A2 (en) 2001-12-07 2003-06-12 Crucell Holland B.V. Production of viruses, viral isolates and vaccines
US20050164386A1 (en) * 1999-04-15 2005-07-28 Uytdehaag Alphonsus G. Overexpression of enzymes involved in post-translational protein modifications in human cells
US7297680B2 (en) * 1999-04-15 2007-11-20 Crucell Holland B.V. Compositions of erythropoietin isoforms comprising Lewis-X structures and high sialic acid content
US7527961B2 (en) 1999-11-26 2009-05-05 Crucell Holland B.V. Production of vaccines
US7192759B1 (en) 1999-11-26 2007-03-20 Crucell Holland B.V. Production of vaccines
US7521220B2 (en) 1999-11-26 2009-04-21 Crucell Holland B.V. Production of vaccines
US20030072737A1 (en) 2000-12-29 2003-04-17 Michael Brines Tissue protective cytokines for the protection, restoration, and enhancement of responsive cells, tissues and organs
US7767643B2 (en) 2000-12-29 2010-08-03 The Kenneth S. Warren Institute, Inc. Protection, restoration, and enhancement of erythropoietin-responsive cells, tissues and organs
CA2465007C (en) * 2001-10-29 2012-01-17 Crucell Holland B.V. Methods and means for producing proteins with predetermined post-translational modifications
US8524477B2 (en) * 2002-10-29 2013-09-03 Crucell Holland B.V. Methods to obtain recombinant proteins with increased sialylation from cells that express adenovirus E1A protein, and proteins obtained thereby
NZ549776A (en) * 2004-03-17 2009-07-31 Crucell Holland Bv Assay for the separation and quantification of hemagglutinin antigens
CA2595676A1 (en) * 2005-01-25 2006-08-03 Apollo Life Sciences Limited Molecules and chimeric molecules thereof
US20100040581A1 (en) * 2005-06-01 2010-02-18 Niigata Tlo Corporation Epo derivative-containing therapeutic agents for blood-related diseases
AR053416A1 (es) * 2005-11-10 2007-05-09 Protech Pharma S A Combinacion de glicoisoformas para el tratamiento o prevencion de la septicemia, linea celular transgenica productora de glicoformas de eritropoyetina, composicion farmaceutica que comprende a dicha combinacion, procedimientos para obtener la linea celular, procedimiento para producir dicha combinac
AU2006316838B2 (en) * 2005-11-15 2012-04-12 Glycofi, Inc Production of glycoproteins with reduced O-glycosylation
US7642078B2 (en) 2005-12-28 2010-01-05 Crucell Holland B.V. Methods to obtain recombinant proteins with increased sialylation from cells that express adenovirus E1A protein, and proteins obtained thereby
US20150038413A1 (en) 2006-02-03 2015-02-05 Opko Biologics Ltd. Long-acting polypeptides and methods of producing and administering same
US7553940B2 (en) * 2006-02-03 2009-06-30 Modigene Inc Long-acting EPO polypeptides and derivatives thereof and methods thereof
US20140113860A1 (en) 2006-02-03 2014-04-24 Prolor Biotech Ltd. Long-acting polypeptides and methods of producing and administering same
US9249407B2 (en) 2006-02-03 2016-02-02 Opko Biologics Ltd. Long-acting coagulation factors and methods of producing same
US8946155B2 (en) 2006-02-03 2015-02-03 Opko Biologics Ltd. Long-acting polypeptides and methods of producing and administering same
US10351615B2 (en) 2006-02-03 2019-07-16 Opko Biologics Ltd. Methods of treatment with long-acting growth hormone
US8048849B2 (en) 2006-02-03 2011-11-01 Modigene, Inc. Long-acting polypeptides and methods of producing same
US10221228B2 (en) 2006-02-03 2019-03-05 Opko Biologics Ltd. Long-acting polypeptides and methods of producing and administering same
WO2007098548A1 (en) * 2006-03-01 2007-09-07 Apollo Life Sciences Limited A molecule and chimeric molecules thereof
CA2654154A1 (en) 2006-06-07 2007-12-13 The University Of Tokushima Treatment of ischemic diseases using erythropoietin
EP2057997A4 (en) 2006-08-22 2012-11-07 Chugai Pharmaceutical Co Ltd MEANS FOR THE PREVENTION AND / OR TREATMENT OF PERIPHERAL NEUROPATHY
AR065613A1 (es) 2007-03-09 2009-06-17 Chugai Pharmaceutical Co Ltd Agentes de proteccion para organos transplantados
BRPI0810065A2 (pt) 2007-04-16 2020-01-14 Momenta Pharmaceuticals Inc caracterização de n-glicanos usando exoglicosidases
TWI488640B (zh) 2008-04-16 2015-06-21 Ferring Int Ct Sa 藥學製劑
US8138092B2 (en) 2009-01-09 2012-03-20 Lam Research Corporation Spacer formation for array double patterning
US9663778B2 (en) 2009-07-09 2017-05-30 OPKO Biologies Ltd. Long-acting coagulation factors and methods of producing same
TWI604850B (zh) 2009-10-05 2017-11-11 菲瑞茵國際中心股份有限公司 藥學製劑
JP6087338B2 (ja) 2011-03-31 2017-03-01 フェリング ベスローテン フェンノートシャップ 医薬製剤
EP2773964A4 (en) * 2011-11-03 2015-06-24 Stc Biolog Inc METHOD FOR DETERMINING THE PHARMACOLOGICAL PROPERTIES OF RECOMBINANT PROTEINS
MY167814A (en) 2012-04-19 2018-09-26 Opko Biologics Ltd Long-acting oxyntomodulin variants and methods of producing same
KR102341581B1 (ko) 2012-11-20 2021-12-21 옵코 바이오로직스 리미티드 생식샘자극 호르몬 카복시 말단 펩타이드들과 부착하여 폴리펩타이드들의 유체역학적 부피를 증가시키는 방법
US20150158926A1 (en) 2013-10-21 2015-06-11 Opko Biologics, Ltd. Long-acting polypeptides and methods of producing and administering same
US20180180626A1 (en) * 2014-07-01 2018-06-28 Stc Biologics, Inc. A method for development of recombinant proteins with fingerprint like similarity to the reference product
WO2016203482A2 (en) 2015-06-19 2016-12-22 Opko Biologics Ltd. Long-acting coagulation factors and methods of producing same
US20190177385A1 (en) * 2016-07-12 2019-06-13 Hexal Ag Glycoprotein with reduced acetylation rate of sialic acid residues
JP7046173B2 (ja) * 2017-10-11 2022-04-01 エランコ・ユーエス・インコーポレイテッド ブタｇ－ｃｓｆ変異体とその使用
CN111514279B (zh) * 2020-06-16 2023-07-18 温州医科大学附属第二医院（温州医科大学附属育英儿童医院） 神经营养因子3在制备治疗男性性腺功能减退症的药物中的应用

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991005867A1 (en) 1989-10-13 1991-05-02 Amgen Inc. Erythropoietin isoforms
US5789247A (en) * 1994-04-01 1998-08-04 Ballay; Annick Expression in non-tumoral human lymphoblastoid lines with an integrative vector
ATE278794T1 (de) 1995-06-15 2004-10-15 Crucell Holland Bv Verpackungssysteme für humane, menschliche adenoviren, zur verwendung in die gentherapie
US6783980B2 (en) * 1995-06-15 2004-08-31 Crucell Holland B.V. Packaging systems for human recombinant adenovirus to be used in gene therapy
ES2239387T3 (es) * 1997-03-27 2005-09-16 The University Of British Columbia Vectores de expresion de insecto.
CA2221819A1 (en) 1997-03-27 1998-09-27 Thomas A. Gigliatti Insect expression vectors
US6929946B1 (en) * 1998-11-20 2005-08-16 Crucell Holland B.V. Gene delivery vectors provided with a tissue tropism for smooth muscle cells, and/or endothelial cells
EA004766B1 (ru) 1999-04-13 2004-08-26 Дзе Кеннет С. Уоррен Инститьют, Инк. Модуляция функции возбуждаемых тканей за счет периферического введения эритропоэтина
ATE536417T1 (de) 1999-04-15 2011-12-15 Crucell Holland Bv Herstellung von rekombinanten proteinen in einer menschlichen zelle mit mindestens einem e1 adenovirus protein
US6855544B1 (en) * 1999-04-15 2005-02-15 Crucell Holland B.V. Recombinant protein production in a human cell
US7297680B2 (en) * 1999-04-15 2007-11-20 Crucell Holland B.V. Compositions of erythropoietin isoforms comprising Lewis-X structures and high sialic acid content
US20050164386A1 (en) * 1999-04-15 2005-07-28 Uytdehaag Alphonsus G. Overexpression of enzymes involved in post-translational protein modifications in human cells
US6913922B1 (en) * 1999-05-18 2005-07-05 Crucell Holland B.V. Serotype of adenovirus and uses thereof
FI109811B (fi) * 2000-09-26 2002-10-15 Medicel Oy Menetelmä GDP-L-fukoosin valmistamiseksi ja menetelmässä käytettäviä välineitä
DE10056136A1 (de) 2000-11-07 2002-05-16 Nemod New Modalities Verwendung von Schwangerschaftsproteinen, Liposomen, nativen Muzin-Fragmenten und Mimikry-Verbindungen zur Behandlung und Prophylaxe von entzündlichen Erkrankungen, zur Verhinderung der Metastasierung und zur Prophylaxe von Tumorerkrankungen
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
ES2411007T3 (es) * 2001-10-10 2013-07-04 Novo Nordisk A/S Remodelación y glicoconjugación de péptidos
CA2465007C (en) * 2001-10-29 2012-01-17 Crucell Holland B.V. Methods and means for producing proteins with predetermined post-translational modifications
ATE514717T1 (de) * 2002-07-18 2011-07-15 Merus B V Rekombinante produktion von antikörpermischungen
NZ549776A (en) * 2004-03-17 2009-07-31 Crucell Holland Bv Assay for the separation and quantification of hemagglutinin antigens
US7183371B2 (en) * 2004-11-01 2007-02-27 General Electric Company Method for making polycarbonate
US20080139608A1 (en) * 2006-12-06 2008-06-12 Universiteit Leiden 2,6,8, Trisubstituted 1-deazapurines and their different uses

Also Published As

Publication number Publication date
CA2465007A1 (en) 2003-05-08
US7785833B2 (en) 2010-08-31
CN100347306C (zh) 2007-11-07
BR0213402A (pt) 2004-10-13
WO2003038100A1 (en) 2003-05-08
IL201673A (en) 2013-10-31
EP1440157B1 (en) 2012-01-25
EP1440157A1 (en) 2004-07-28
EA012340B1 (ru) 2009-10-30
US20080032922A1 (en) 2008-02-07
CN1578838A (zh) 2005-02-09
JP2005507426A (ja) 2005-03-17
IL201674A (en) 2013-10-31
EP2292770A3 (en) 2012-12-19
JP4583029B2 (ja) 2010-11-17
EP2292770A2 (en) 2011-03-09
US20070117742A1 (en) 2007-05-24
US7696157B2 (en) 2010-04-13
EA008220B1 (ru) 2007-04-27
EA200602163A1 (ru) 2007-04-27
EP2292770B1 (en) 2014-11-26
IL161674A (en) 2014-06-30
US20050164917A1 (en) 2005-07-28
AU2002335585B2 (en) 2007-08-16
NO20042209L (no) 2004-07-28
US7304031B2 (en) 2007-12-04
ES2381104T3 (es) 2012-05-23
CA2465007C (en) 2012-01-17
EA200400605A1 (ru) 2004-08-26
ATE542904T1 (de) 2012-02-15
CA2756610C (en) 2015-08-25
CA2756610A1 (en) 2003-05-08

Similar Documents

Publication Publication Date Title
DK1440157T3 (da) Methods and means for producing proteins with predetermined post-translational modifications
DE59608743D1 (de) Verfahren zur herstellung von rekombinanten proteinen in e. coli mittels hochzelldichte-fermentation
ATE294229T1 (de) Verfahren zur trocknung von kompetenten zellen
DE68921364D1 (de) Verfahren zur Herstellung von Proteinen mittels rekombinanter DNA.
DE69434578D1 (de) Expressionsvektoren die für bispezifische Proteine kodieren, und Verfahren zur Herstellung von biologisch-aktiven bispezifischen Fusionsproteine in Zellen von Säugertieren
ATE289354T1 (de) Verwendung von adenovirus e1 protein sequenzen kodierenden, zur herstellung von rekombinanten proteinen in menschlichen zellen.
ATE500331T1 (de) Verfahren zur gleichzeitigen herstellung von multiplen proteinen; vektoren und zellen, die hierfür verwendung finden
ATE140960T1 (de) Verfahren zur herstellung von zellen, die stabil integrierte fremde dns mit hoher kopiezahl enthalten, durch dieses verfahren hergestellte zellen und verwendung dieser zellen zur herstellung von durch diese fremde dns kodierten polypeptiden
ATE91716T1 (de) Verfahren zur herstellung vielkettige polypeptide oder proteine.
DK1042461T3 (da) Ekspressionskloning i filiforme svampe
ATE430197T1 (de) Fibroblasten wachstumsfaktor-ähnliche polypeptide
ATE475708T1 (de) Fusionskonstrukte und deren verwendung zur produktion von antikörpern mit erhöhter fc rezeptor bindungsaffinität und effektorfunktion
KR910004803A (ko) 간의 실질 세포 성장 인자, 이를 코딩하는 유전자, 이 인자를 생산하는 방법, 및 이 인자를 생산하는 형질전환체
ATE278024T1 (de) Methode zur herstellung von rekombinanten zellen
ATE203270T1 (de) Verfahren für die herstellung von proteinen
DE69939268D1 (de) Methoden zur aufreinigung von rekombinatem humanem erythropoietin aus zellkulturüberständen
PT1025245E (pt) Criacao rapida de linhas celulares estaveis de mamifero produzindo elevados iiveis de proteinas recombinantes
DE60122562D1 (de) Nukleinsäuren, die für stichodactylidae chromoproteine kodieren
ATE328102T1 (de) Verfahren zur herstellung von proteine
ATE506370T1 (de) Reagenzien und verfahren zur bildung von disulfid-bindungen und glykosylierung von proteinen
ATE532793T1 (de) Verfahren zur herstellung von molekülen mit verminderter immunogenität
ATE435277T1 (de) 18477, eine menschliche proteinkinase und deren verwendung
DE69939876D1 (de) Methoden zur massenkultivierung von zellen die rekombinantes menschliches erythropoietin produzieren
DE69530261D1 (de) Durch Zelldichte stimulierte Protein-Tyrosin-Phosphatasen
ATE288476T1 (de) Herstellung von proteinen