DK1078081T3 - Fremgangsmåde til fremstilling af heterologe polypeptider i trichothecen deficiente filamentöse svampe mutantceller - Google Patents
Fremgangsmåde til fremstilling af heterologe polypeptider i trichothecen deficiente filamentöse svampe mutantcellerInfo
- Publication number
- DK1078081T3 DK1078081T3 DK99925717T DK99925717T DK1078081T3 DK 1078081 T3 DK1078081 T3 DK 1078081T3 DK 99925717 T DK99925717 T DK 99925717T DK 99925717 T DK99925717 T DK 99925717T DK 1078081 T3 DK1078081 T3 DK 1078081T3
- Authority
- DK
- Denmark
- Prior art keywords
- nucleic acid
- filamentous fungal
- mutant
- cells
- trichothecene
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 229920001184 polypeptide Polymers 0.000 title abstract 5
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 5
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 5
- 230000002538 fungal effect Effects 0.000 title abstract 4
- LZAJKCZTKKKZNT-PMNGPLLRSA-N trichothecene Chemical compound C12([C@@]3(CC[C@H]2OC2C=C(CCC23C)C)C)CO1 LZAJKCZTKKKZNT-PMNGPLLRSA-N 0.000 title abstract 3
- 229930013292 trichothecene Natural products 0.000 title abstract 3
- 230000002950 deficient Effects 0.000 title 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 5
- 108700023225 Trichodiene synthases Proteins 0.000 abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 238000012986 modification Methods 0.000 abstract 2
- 230000004048 modification Effects 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
- C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
- C12Y402/03006—Trichodiene synthase (4.2.3.6)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8221798A | 1998-05-20 | 1998-05-20 | |
| PCT/US1999/011223 WO1999060137A2 (en) | 1998-05-20 | 1999-05-20 | Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1078081T3 true DK1078081T3 (da) | 2008-08-04 |
Family
ID=22169802
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK99925717T DK1078081T3 (da) | 1998-05-20 | 1999-05-20 | Fremgangsmåde til fremstilling af heterologe polypeptider i trichothecen deficiente filamentöse svampe mutantceller |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US6180366B1 (https=) |
| EP (1) | EP1078081B1 (https=) |
| JP (2) | JP2002515253A (https=) |
| CN (2) | CN1231579C (https=) |
| AT (1) | ATE395424T1 (https=) |
| AU (1) | AU4195099A (https=) |
| DE (1) | DE69938722D1 (https=) |
| DK (1) | DK1078081T3 (https=) |
| WO (1) | WO1999060137A2 (https=) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6060305A (en) * | 1994-06-30 | 2000-05-09 | Novo Nordisk Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system |
| WO1999055396A1 (en) * | 1998-04-27 | 1999-11-04 | Surmodics, Inc. | Bioactive agent release coating |
| JP2002515253A (ja) * | 1998-05-20 | 2002-05-28 | ノボザイムス バイオテック，インコーポレイティド | トリコテセン−欠失糸状菌変異体細胞において異種ポリベプチドを生成するための方法 |
| FR2826978B1 (fr) * | 2001-07-06 | 2004-09-17 | Agronomique Inst Nat Rech | Procede de determination de la capacite de fusarium a produire des toxines trichotecenes |
| AU2003231028A1 (en) * | 2002-04-22 | 2003-11-03 | Novozymes Biotech, Inc. | Methods for increasing homologous recombination of a nucleic acid sequence |
| CN1694953B (zh) | 2002-08-30 | 2010-05-12 | 诺维信股份有限公司 | 制备哺乳动物胰蛋白酶的方法 |
| AU2003258395A1 (en) * | 2002-08-30 | 2004-03-19 | Puratos Naamloze Vennootschap | Myrothecium sp. transformation and expression system |
| CN100480391C (zh) | 2002-11-18 | 2009-04-22 | 诺维信股份有限公司 | 用于在真菌细胞中表达基因的启动子变体 |
| US20090233287A1 (en) * | 2006-04-25 | 2009-09-17 | Roelof Ary Lans Bovenberg | Production of compounds in a recombinant host |
| KR20090017608A (ko) * | 2006-05-18 | 2009-02-18 | 코닌클리케 필립스 일렉트로닉스 엔.브이. | 상부 가열수단이 상대적으로 작은 수평 토스트 장치 |
| EP2352831B1 (en) | 2008-09-30 | 2015-03-25 | Novozymes Inc. | Methods for producing polypeptides in enzyme-deficient mutants of fusarium venenatum |
| US20100316764A1 (en) * | 2009-06-10 | 2010-12-16 | Engrain, LLC | Flour supplement compositions and methods for preparing wheat flour |
| CN102695787A (zh) * | 2009-08-05 | 2012-09-26 | 诺维信公司 | 用于从木素纤维素原料产生trichodiene的丝状真菌和方法 |
| DK2513290T3 (en) | 2009-12-18 | 2016-01-25 | Novozymes Inc | Methods for Preparation of Polypeptides in Trichoderma Mutants with Protease Deficiency |
| CN108588060B (zh) * | 2017-03-07 | 2020-12-01 | 武汉康复得生物科技股份有限公司 | 一种用丝状真菌宿主细胞表达的重组草酸脱羧酶 |
| JP2021078354A (ja) | 2018-03-08 | 2021-05-27 | 有限会社アルティザイム・インターナショナル | フラビンアデニンジヌクレオチドグルコース脱水素酵素とシトクロム分子との融合タンパク質 |
| CN112522231B (zh) * | 2020-12-14 | 2023-03-14 | 广东省微生物研究所（广东省微生物分析检测中心） | 一种酰基转移酶及其编码基因和应用 |
| US20250008961A1 (en) | 2021-11-22 | 2025-01-09 | Basf Se | Paenibacillus strains producing low amounts of dab comprising compounds |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8904762D0 (en) * | 1989-03-02 | 1989-04-12 | Glaxo Group Ltd | Biological process |
| CN101659926A (zh) * | 1994-06-30 | 2010-03-03 | 诺沃奇梅兹有限公司 | 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子 |
| US5594119A (en) * | 1994-09-20 | 1997-01-14 | Novo Nordisk A/S | Gene encoding carboxypeptidase of aspergillus niger |
| JP3628336B2 (ja) * | 1996-03-11 | 2005-03-09 | 明治製菓株式会社 | β―フルクトフラノシダーゼおよびその遺伝子、β―フルクトフラノシダーゼ遺伝子の単離法、β―フルクトフラノシダーゼの産生系、並びにβ―フルクトフラノシダーゼ変異体 |
| JP2002515253A (ja) * | 1998-05-20 | 2002-05-28 | ノボザイムス バイオテック，インコーポレイティド | トリコテセン−欠失糸状菌変異体細胞において異種ポリベプチドを生成するための方法 |
| US6383781B1 (en) * | 1998-12-23 | 2002-05-07 | Novozymes A/S | Methods for producing polypeptides in aspergillus mutant cells |
-
1999
- 1999-05-20 JP JP2000549743A patent/JP2002515253A/ja active Pending
- 1999-05-20 AT AT99925717T patent/ATE395424T1/de not_active IP Right Cessation
- 1999-05-20 CN CNB031588026A patent/CN1231579C/zh not_active Expired - Fee Related
- 1999-05-20 WO PCT/US1999/011223 patent/WO1999060137A2/en not_active Ceased
- 1999-05-20 US US09/316,080 patent/US6180366B1/en not_active Expired - Lifetime
- 1999-05-20 DK DK99925717T patent/DK1078081T3/da active
- 1999-05-20 CN CNB998081124A patent/CN1190495C/zh not_active Expired - Fee Related
- 1999-05-20 AU AU41950/99A patent/AU4195099A/en not_active Abandoned
- 1999-05-20 DE DE69938722T patent/DE69938722D1/de not_active Expired - Lifetime
- 1999-05-20 EP EP99925717A patent/EP1078081B1/en not_active Expired - Lifetime
-
2005
- 2005-03-31 US US11/095,491 patent/US7332341B2/en not_active Expired - Fee Related
-
2008
- 2008-01-31 US US12/023,417 patent/US20080213901A1/en not_active Abandoned
-
2009
- 2009-08-19 JP JP2009190191A patent/JP2009291207A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20050181480A1 (en) | 2005-08-18 |
| DE69938722D1 (de) | 2008-06-26 |
| CN1500882A (zh) | 2004-06-02 |
| EP1078081B1 (en) | 2008-05-14 |
| CN1190495C (zh) | 2005-02-23 |
| CN1231579C (zh) | 2005-12-14 |
| CN1309711A (zh) | 2001-08-22 |
| ATE395424T1 (de) | 2008-05-15 |
| JP2009291207A (ja) | 2009-12-17 |
| US20080213901A1 (en) | 2008-09-04 |
| EP1078081A2 (en) | 2001-02-28 |
| AU4195099A (en) | 1999-12-06 |
| JP2002515253A (ja) | 2002-05-28 |
| WO1999060137A2 (en) | 1999-11-25 |
| US6180366B1 (en) | 2001-01-30 |
| WO1999060137A3 (en) | 2000-05-18 |
| US7332341B2 (en) | 2008-02-19 |
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