CN1231579C - trichodiene合成酶及其制备方法 - Google Patents

trichodiene合成酶及其制备方法 Download PDF

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Publication number
CN1231579C
CN1231579C CNB031588026A CN03158802A CN1231579C CN 1231579 C CN1231579 C CN 1231579C CN B031588026 A CNB031588026 A CN B031588026A CN 03158802 A CN03158802 A CN 03158802A CN 1231579 C CN1231579 C CN 1231579C
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Prior art keywords
nucleic acid
gene
acid sequence
polypeptide
fusarium
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Expired - Fee Related
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CNB031588026A
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English (en)
Chinese (zh)
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CN1500882A (zh
Inventor
J·C·罗耶
L·M·克里斯琴森
G·A·甘贝塔
H·布鲁迪
S·M·奥塔尼
W·T·约德
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Novozymes AS
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Novozymes Biotech Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03006—Trichodiene synthase (4.2.3.6)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
CNB031588026A 1998-05-20 1999-05-20 trichodiene合成酶及其制备方法 Expired - Fee Related CN1231579C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8221798A 1998-05-20 1998-05-20
US09/082,217 1998-05-20

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CNB998081124A Division CN1190495C (zh) 1998-05-20 1999-05-20 在单端孢菌毒素缺陷的丝状真菌突变细胞中生产异源多肽的方法

Publications (2)

Publication Number Publication Date
CN1500882A CN1500882A (zh) 2004-06-02
CN1231579C true CN1231579C (zh) 2005-12-14

Family

ID=22169802

Family Applications (2)

Application Number Title Priority Date Filing Date
CNB031588026A Expired - Fee Related CN1231579C (zh) 1998-05-20 1999-05-20 trichodiene合成酶及其制备方法
CNB998081124A Expired - Fee Related CN1190495C (zh) 1998-05-20 1999-05-20 在单端孢菌毒素缺陷的丝状真菌突变细胞中生产异源多肽的方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CNB998081124A Expired - Fee Related CN1190495C (zh) 1998-05-20 1999-05-20 在单端孢菌毒素缺陷的丝状真菌突变细胞中生产异源多肽的方法

Country Status (9)

Country Link
US (3) US6180366B1 (https=)
EP (1) EP1078081B1 (https=)
JP (2) JP2002515253A (https=)
CN (2) CN1231579C (https=)
AT (1) ATE395424T1 (https=)
AU (1) AU4195099A (https=)
DE (1) DE69938722D1 (https=)
DK (1) DK1078081T3 (https=)
WO (1) WO1999060137A2 (https=)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6060305A (en) * 1994-06-30 2000-05-09 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system
WO1999055396A1 (en) * 1998-04-27 1999-11-04 Surmodics, Inc. Bioactive agent release coating
DE69938722D1 (de) * 1998-05-20 2008-06-26 Novozymes Inc Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen
FR2826978B1 (fr) * 2001-07-06 2004-09-17 Agronomique Inst Nat Rech Procede de determination de la capacite de fusarium a produire des toxines trichotecenes
AU2003231028A1 (en) * 2002-04-22 2003-11-03 Novozymes Biotech, Inc. Methods for increasing homologous recombination of a nucleic acid sequence
WO2004020611A1 (en) * 2002-08-30 2004-03-11 Puratos Naamloze Vennootschap Myrothecium sp. transformation and expression system
WO2004020612A1 (en) 2002-08-30 2004-03-11 Novozymes Biotech, Inc. Methods for producing mammalian trypsins
WO2004046373A2 (en) 2002-11-18 2004-06-03 Novozymes Biotech, Inc. Promoter variants for expressing genes in a fungal cell
US20090233287A1 (en) * 2006-04-25 2009-09-17 Roelof Ary Lans Bovenberg Production of compounds in a recombinant host
KR20090017608A (ko) * 2006-05-18 2009-02-18 코닌클리케 필립스 일렉트로닉스 엔.브이. 상부 가열수단이 상대적으로 작은 수평 토스트 장치
ES2538790T3 (es) 2008-09-30 2015-06-24 Novozymes Inc. Métodos para la producción de polipéptidos en mutantes de Fusarium venenatum deficientes en enzimas
US20100316764A1 (en) * 2009-06-10 2010-12-16 Engrain, LLC Flour supplement compositions and methods for preparing wheat flour
WO2011017549A2 (en) * 2009-08-05 2011-02-10 Dorsan Biofuels, Inc. Filamentous fungi and methods for producing trichodiene from lignocellulosic feedstocks
MX350399B (es) 2009-12-18 2017-09-06 Novozymes Inc Metodos para producir polipeptidos en mutantes de trichoderma deficientes en proteasa.
CN108588060B (zh) * 2017-03-07 2020-12-01 武汉康复得生物科技股份有限公司 一种用丝状真菌宿主细胞表达的重组草酸脱羧酶
JP2021078354A (ja) * 2018-03-08 2021-05-27 有限会社アルティザイム・インターナショナル フラビンアデニンジヌクレオチドグルコース脱水素酵素とシトクロム分子との融合タンパク質
CN112522231B (zh) * 2020-12-14 2023-03-14 广东省微生物研究所（广东省微生物分析检测中心） 一种酰基转移酶及其编码基因和应用
WO2023088791A1 (en) 2021-11-22 2023-05-25 Basf Se Paenibacillus strains producing low amounts of dab comprising compounds.

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8904762D0 (en) * 1989-03-02 1989-04-12 Glaxo Group Ltd Biological process
ATE294871T1 (de) * 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
US5594119A (en) * 1994-09-20 1997-01-14 Novo Nordisk A/S Gene encoding carboxypeptidase of aspergillus niger
WO1997034004A1 (en) * 1996-03-11 1997-09-18 Meiji Seika Kaisha, Ltd. β-FRUCTOFURANOSIDASE AND ITS GENE, METHOD OF ISOLATING β-FRUCTOFURANOSIDASE GENE, SYSTEM FOR PRODUCING β-FRUCTOFURANOSIDASE, AND β-FRUCTOFURANOSIDASE VARIANT
DE69938722D1 (de) * 1998-05-20 2008-06-26 Novozymes Inc Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen
US6383781B1 (en) * 1998-12-23 2002-05-07 Novozymes A/S Methods for producing polypeptides in aspergillus mutant cells

Also Published As

Publication number Publication date
US20080213901A1 (en) 2008-09-04
US7332341B2 (en) 2008-02-19
WO1999060137A2 (en) 1999-11-25
DK1078081T3 (da) 2008-08-04
DE69938722D1 (de) 2008-06-26
US20050181480A1 (en) 2005-08-18
ATE395424T1 (de) 2008-05-15
CN1190495C (zh) 2005-02-23
EP1078081A2 (en) 2001-02-28
AU4195099A (en) 1999-12-06
EP1078081B1 (en) 2008-05-14
WO1999060137A3 (en) 2000-05-18
JP2002515253A (ja) 2002-05-28
CN1309711A (zh) 2001-08-22
CN1500882A (zh) 2004-06-02
JP2009291207A (ja) 2009-12-17
US6180366B1 (en) 2001-01-30

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Owner name: NUOWOQIMEIZI CO.,LTD.

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Termination date: 20170520