CN1231579C - trichodiene合成酶及其制备方法 - Google Patents

trichodiene合成酶及其制备方法 Download PDF

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Publication number
CN1231579C
CN1231579C CNB031588026A CN03158802A CN1231579C CN 1231579 C CN1231579 C CN 1231579C CN B031588026 A CNB031588026 A CN B031588026A CN 03158802 A CN03158802 A CN 03158802A CN 1231579 C CN1231579 C CN 1231579C
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Prior art keywords
nucleic acid
gene
acid sequence
polypeptide
fusarium
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Expired - Fee Related
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CNB031588026A
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English (en)
Chinese (zh)
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CN1500882A (zh
Inventor
J·C·罗耶
L·M·克里斯琴森
G·A·甘贝塔
H·布鲁迪
S·M·奥塔尼
W·T·约德
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Novozymes AS
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Novozymes Biotech Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03006—Trichodiene synthase (4.2.3.6)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
CNB031588026A 1998-05-20 1999-05-20 trichodiene合成酶及其制备方法 Expired - Fee Related CN1231579C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8221798A 1998-05-20 1998-05-20
US09/082,217 1998-05-20

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CNB998081124A Division CN1190495C (zh) 1998-05-20 1999-05-20 在单端孢菌毒素缺陷的丝状真菌突变细胞中生产异源多肽的方法

Publications (2)

Publication Number Publication Date
CN1500882A CN1500882A (zh) 2004-06-02
CN1231579C true CN1231579C (zh) 2005-12-14

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ID=22169802

Family Applications (2)

Application Number Title Priority Date Filing Date
CNB031588026A Expired - Fee Related CN1231579C (zh) 1998-05-20 1999-05-20 trichodiene合成酶及其制备方法
CNB998081124A Expired - Fee Related CN1190495C (zh) 1998-05-20 1999-05-20 在单端孢菌毒素缺陷的丝状真菌突变细胞中生产异源多肽的方法

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CNB998081124A Expired - Fee Related CN1190495C (zh) 1998-05-20 1999-05-20 在单端孢菌毒素缺陷的丝状真菌突变细胞中生产异源多肽的方法

Country Status (9)

Country Link
US (3) US6180366B1 (https=)
EP (1) EP1078081B1 (https=)
JP (2) JP2002515253A (https=)
CN (2) CN1231579C (https=)
AT (1) ATE395424T1 (https=)
AU (1) AU4195099A (https=)
DE (1) DE69938722D1 (https=)
DK (1) DK1078081T3 (https=)
WO (1) WO1999060137A2 (https=)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6060305A (en) * 1994-06-30 2000-05-09 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system
WO1999055396A1 (en) * 1998-04-27 1999-11-04 Surmodics, Inc. Bioactive agent release coating
JP2002515253A (ja) * 1998-05-20 2002-05-28 ノボザイムス バイオテック，インコーポレイティド トリコテセン−欠失糸状菌変異体細胞において異種ポリベプチドを生成するための方法
FR2826978B1 (fr) * 2001-07-06 2004-09-17 Agronomique Inst Nat Rech Procede de determination de la capacite de fusarium a produire des toxines trichotecenes
AU2003231028A1 (en) * 2002-04-22 2003-11-03 Novozymes Biotech, Inc. Methods for increasing homologous recombination of a nucleic acid sequence
CN1694953B (zh) 2002-08-30 2010-05-12 诺维信股份有限公司 制备哺乳动物胰蛋白酶的方法
AU2003258395A1 (en) * 2002-08-30 2004-03-19 Puratos Naamloze Vennootschap Myrothecium sp. transformation and expression system
CN100480391C (zh) 2002-11-18 2009-04-22 诺维信股份有限公司 用于在真菌细胞中表达基因的启动子变体
US20090233287A1 (en) * 2006-04-25 2009-09-17 Roelof Ary Lans Bovenberg Production of compounds in a recombinant host
KR20090017608A (ko) * 2006-05-18 2009-02-18 코닌클리케 필립스 일렉트로닉스 엔.브이. 상부 가열수단이 상대적으로 작은 수평 토스트 장치
EP2352831B1 (en) 2008-09-30 2015-03-25 Novozymes Inc. Methods for producing polypeptides in enzyme-deficient mutants of fusarium venenatum
US20100316764A1 (en) * 2009-06-10 2010-12-16 Engrain, LLC Flour supplement compositions and methods for preparing wheat flour
CN102695787A (zh) * 2009-08-05 2012-09-26 诺维信公司 用于从木素纤维素原料产生trichodiene的丝状真菌和方法
DK2513290T3 (en) 2009-12-18 2016-01-25 Novozymes Inc Methods for Preparation of Polypeptides in Trichoderma Mutants with Protease Deficiency
CN108588060B (zh) * 2017-03-07 2020-12-01 武汉康复得生物科技股份有限公司 一种用丝状真菌宿主细胞表达的重组草酸脱羧酶
JP2021078354A (ja) 2018-03-08 2021-05-27 有限会社アルティザイム・インターナショナル フラビンアデニンジヌクレオチドグルコース脱水素酵素とシトクロム分子との融合タンパク質
CN112522231B (zh) * 2020-12-14 2023-03-14 广东省微生物研究所（广东省微生物分析检测中心） 一种酰基转移酶及其编码基因和应用
US20250008961A1 (en) 2021-11-22 2025-01-09 Basf Se Paenibacillus strains producing low amounts of dab comprising compounds

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8904762D0 (en) * 1989-03-02 1989-04-12 Glaxo Group Ltd Biological process
CN101659926A (zh) * 1994-06-30 2010-03-03 诺沃奇梅兹有限公司 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子
US5594119A (en) * 1994-09-20 1997-01-14 Novo Nordisk A/S Gene encoding carboxypeptidase of aspergillus niger
JP3628336B2 (ja) * 1996-03-11 2005-03-09 明治製菓株式会社 β―フルクトフラノシダーゼおよびその遺伝子、β―フルクトフラノシダーゼ遺伝子の単離法、β―フルクトフラノシダーゼの産生系、並びにβ―フルクトフラノシダーゼ変異体
JP2002515253A (ja) * 1998-05-20 2002-05-28 ノボザイムス バイオテック，インコーポレイティド トリコテセン−欠失糸状菌変異体細胞において異種ポリベプチドを生成するための方法
US6383781B1 (en) * 1998-12-23 2002-05-07 Novozymes A/S Methods for producing polypeptides in aspergillus mutant cells

Also Published As

Publication number Publication date
US20050181480A1 (en) 2005-08-18
DE69938722D1 (de) 2008-06-26
CN1500882A (zh) 2004-06-02
EP1078081B1 (en) 2008-05-14
CN1190495C (zh) 2005-02-23
DK1078081T3 (da) 2008-08-04
CN1309711A (zh) 2001-08-22
ATE395424T1 (de) 2008-05-15
JP2009291207A (ja) 2009-12-17
US20080213901A1 (en) 2008-09-04
EP1078081A2 (en) 2001-02-28
AU4195099A (en) 1999-12-06
JP2002515253A (ja) 2002-05-28
WO1999060137A2 (en) 1999-11-25
US6180366B1 (en) 2001-01-30
WO1999060137A3 (en) 2000-05-18
US7332341B2 (en) 2008-02-19

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