AU4195099A - Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells - Google Patents

Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells

Info

Publication number
AU4195099A
AU4195099A AU41950/99A AU4195099A AU4195099A AU 4195099 A AU4195099 A AU 4195099A AU 41950/99 A AU41950/99 A AU 41950/99A AU 4195099 A AU4195099 A AU 4195099A AU 4195099 A AU4195099 A AU 4195099A
Authority
AU
Australia
Prior art keywords
methods
nucleic acid
filamentous fungal
mutant
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU41950/99A
Other languages
English (en)
Inventor
Howard Brody
Lynne M. Christianson
Gregory A. Gambetta
Suzanne M. Otani
John C Royer
Wendy T. Yoder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes Inc
Original Assignee
Novo Nordisk Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk Biotech Inc filed Critical Novo Nordisk Biotech Inc
Publication of AU4195099A publication Critical patent/AU4195099A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03006—Trichodiene synthase (4.2.3.6)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
AU41950/99A 1998-05-20 1999-05-20 Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells Abandoned AU4195099A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US8221798A 1998-05-20 1998-05-20
US09082217 1998-05-20
PCT/US1999/011223 WO1999060137A2 (en) 1998-05-20 1999-05-20 Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells

Publications (1)

Publication Number Publication Date
AU4195099A true AU4195099A (en) 1999-12-06

Family

ID=22169802

Family Applications (1)

Application Number Title Priority Date Filing Date
AU41950/99A Abandoned AU4195099A (en) 1998-05-20 1999-05-20 Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells

Country Status (9)

Country Link
US (3) US6180366B1 (https=)
EP (1) EP1078081B1 (https=)
JP (2) JP2002515253A (https=)
CN (2) CN1231579C (https=)
AT (1) ATE395424T1 (https=)
AU (1) AU4195099A (https=)
DE (1) DE69938722D1 (https=)
DK (1) DK1078081T3 (https=)
WO (1) WO1999060137A2 (https=)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6060305A (en) * 1994-06-30 2000-05-09 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system
WO1999055396A1 (en) * 1998-04-27 1999-11-04 Surmodics, Inc. Bioactive agent release coating
JP2002515253A (ja) * 1998-05-20 2002-05-28 ノボザイムス バイオテック，インコーポレイティド トリコテセン−欠失糸状菌変異体細胞において異種ポリベプチドを生成するための方法
FR2826978B1 (fr) * 2001-07-06 2004-09-17 Agronomique Inst Nat Rech Procede de determination de la capacite de fusarium a produire des toxines trichotecenes
AU2003231028A1 (en) * 2002-04-22 2003-11-03 Novozymes Biotech, Inc. Methods for increasing homologous recombination of a nucleic acid sequence
CN1694953B (zh) 2002-08-30 2010-05-12 诺维信股份有限公司 制备哺乳动物胰蛋白酶的方法
AU2003258395A1 (en) * 2002-08-30 2004-03-19 Puratos Naamloze Vennootschap Myrothecium sp. transformation and expression system
CN100480391C (zh) 2002-11-18 2009-04-22 诺维信股份有限公司 用于在真菌细胞中表达基因的启动子变体
US20090233287A1 (en) * 2006-04-25 2009-09-17 Roelof Ary Lans Bovenberg Production of compounds in a recombinant host
KR20090017608A (ko) * 2006-05-18 2009-02-18 코닌클리케 필립스 일렉트로닉스 엔.브이. 상부 가열수단이 상대적으로 작은 수평 토스트 장치
EP2352831B1 (en) 2008-09-30 2015-03-25 Novozymes Inc. Methods for producing polypeptides in enzyme-deficient mutants of fusarium venenatum
US20100316764A1 (en) * 2009-06-10 2010-12-16 Engrain, LLC Flour supplement compositions and methods for preparing wheat flour
CN102695787A (zh) * 2009-08-05 2012-09-26 诺维信公司 用于从木素纤维素原料产生trichodiene的丝状真菌和方法
DK2513290T3 (en) 2009-12-18 2016-01-25 Novozymes Inc Methods for Preparation of Polypeptides in Trichoderma Mutants with Protease Deficiency
CN108588060B (zh) * 2017-03-07 2020-12-01 武汉康复得生物科技股份有限公司 一种用丝状真菌宿主细胞表达的重组草酸脱羧酶
JP2021078354A (ja) 2018-03-08 2021-05-27 有限会社アルティザイム・インターナショナル フラビンアデニンジヌクレオチドグルコース脱水素酵素とシトクロム分子との融合タンパク質
CN112522231B (zh) * 2020-12-14 2023-03-14 广东省微生物研究所（广东省微生物分析检测中心） 一种酰基转移酶及其编码基因和应用
US20250008961A1 (en) 2021-11-22 2025-01-09 Basf Se Paenibacillus strains producing low amounts of dab comprising compounds

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8904762D0 (en) * 1989-03-02 1989-04-12 Glaxo Group Ltd Biological process
CN101659926A (zh) * 1994-06-30 2010-03-03 诺沃奇梅兹有限公司 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子
US5594119A (en) * 1994-09-20 1997-01-14 Novo Nordisk A/S Gene encoding carboxypeptidase of aspergillus niger
JP3628336B2 (ja) * 1996-03-11 2005-03-09 明治製菓株式会社 β―フルクトフラノシダーゼおよびその遺伝子、β―フルクトフラノシダーゼ遺伝子の単離法、β―フルクトフラノシダーゼの産生系、並びにβ―フルクトフラノシダーゼ変異体
JP2002515253A (ja) * 1998-05-20 2002-05-28 ノボザイムス バイオテック，インコーポレイティド トリコテセン−欠失糸状菌変異体細胞において異種ポリベプチドを生成するための方法
US6383781B1 (en) * 1998-12-23 2002-05-07 Novozymes A/S Methods for producing polypeptides in aspergillus mutant cells

Also Published As

Publication number Publication date
US20050181480A1 (en) 2005-08-18
DE69938722D1 (de) 2008-06-26
CN1500882A (zh) 2004-06-02
EP1078081B1 (en) 2008-05-14
CN1190495C (zh) 2005-02-23
CN1231579C (zh) 2005-12-14
DK1078081T3 (da) 2008-08-04
CN1309711A (zh) 2001-08-22
ATE395424T1 (de) 2008-05-15
JP2009291207A (ja) 2009-12-17
US20080213901A1 (en) 2008-09-04
EP1078081A2 (en) 2001-02-28
JP2002515253A (ja) 2002-05-28
WO1999060137A2 (en) 1999-11-25
US6180366B1 (en) 2001-01-30
WO1999060137A3 (en) 2000-05-18
US7332341B2 (en) 2008-02-19

Similar Documents

Publication Publication Date Title
AU4195099A (en) Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
DE69939947D1 (de) Verfahren zur herstellung von polypeptiden in mutierten aspergillus zellen
WO2000056900A3 (en) Promoter sequences derived from fusarium venenatum and uses thereof
Gleeson et al. Genetic analysis in the methylotrophic yeast Hansenula polymorpha
AU5445098A (en) Methods for producing polypeptides in surfactin mutants of bacillus cells
WO2003066818A3 (en) Secretion, transcription and sporulation genes in bacillus clausii
WO1998014602A3 (en) Preparation of d-amino acids by direct fermentative means
WO2007015848A3 (en) Improved methods and compositions for increasing longevity and protein yield from a cell culture
AU4200897A (en) Novel host cells and methods of producing proteins
US4816405A (en) Vectors for transformation by ascomycetes
CN115029257A (zh) 产β-胡萝卜素的重组解脂耶氏酵母及其构建方法和应用
AU2003294397A1 (en) Promoter variants for expressing genes in a fungal cell
AU4940096A (en) Host cell expressing reduced levels of a metalloprotease and methods using the host cell in protein production
EP0118367A2 (en) Recombinant DNA cloning vector pVE1, deletion and hybrid mutants, and recombinant derivatives thereof, products and processes
WO2004011609A3 (en) Methods for producing biological substances in pigment-deficient mutants of bacillus cells
EP2298911A3 (en) Methods for producing polypeptides in respiratory-deficient cells
WO2000046375A3 (en) Methods for producing polypeptides in fungal cells
WO1999054477A3 (en) Genetically engineered yeast and mutants thereof for the efficient fermentation of lignocellulose hydrolysates
WO2000042203A3 (en) Methods for producing polypeptides in cyclohexadepsipeptide-deficient cells
AU2002224554A1 (en) Cytochrome b5 gene and protein of candida tropicalis and methods relating thereto
PT1383890E (pt) Mutantes da desoxicitidina-quinase possuindo uma actividade enzimática aumentada
EP1062318B1 (en) A prokaryotic cell comprising two copies of a gene transcribed in different directions
WO2001051646A3 (en) Methods for producing a polypeptide using a crippled translational initiator sequence
ATE451469T1 (de) In vivo-verfahren zur herstellung von proteinen, die nicht-konventionelle aminosäuren enthalten
Royer et al. Methods for producing heterologous polypeptides in trichothecene-deficient fungi

Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase