DK0948630T3 - Kloning og karakterisering af napsin, en asparaginprotease - Google Patents
Kloning og karakterisering af napsin, en asparaginproteaseInfo
- Publication number
- DK0948630T3 DK0948630T3 DK97948546T DK97948546T DK0948630T3 DK 0948630 T3 DK0948630 T3 DK 0948630T3 DK 97948546 T DK97948546 T DK 97948546T DK 97948546 T DK97948546 T DK 97948546T DK 0948630 T3 DK0948630 T3 DK 0948630T3
- Authority
- DK
- Denmark
- Prior art keywords
- napsin
- enzyme
- protease
- cloned
- expressed
- Prior art date
Links
- 108091005804 Peptidases Proteins 0.000 title abstract 3
- 239000004365 Protease Substances 0.000 title abstract 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title abstract 3
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 title 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 title 1
- 229960001230 asparagine Drugs 0.000 title 1
- 235000009582 asparagine Nutrition 0.000 title 1
- 238000012512 characterization method Methods 0.000 title 1
- 238000010367 cloning Methods 0.000 title 1
- 102000004190 Enzymes Human genes 0.000 abstract 5
- 108090000790 Enzymes Proteins 0.000 abstract 5
- 102000035101 Aspartic proteases Human genes 0.000 abstract 2
- 108091005502 Aspartic proteases Proteins 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000003908 Cathepsin D Human genes 0.000 abstract 1
- 108090000258 Cathepsin D Proteins 0.000 abstract 1
- 108010044467 Isoenzymes Proteins 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 210000004185 liver Anatomy 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 210000005084 renal tissue Anatomy 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US3119696P | 1996-11-20 | 1996-11-20 | |
| US4612697P | 1997-05-09 | 1997-05-09 | |
| PCT/US1997/021684 WO1998022597A2 (en) | 1996-11-20 | 1997-11-20 | Cloning and characterization of napsin, an aspartic protease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK0948630T3 true DK0948630T3 (da) | 2008-08-11 |
Family
ID=26706937
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK97948546T DK0948630T3 (da) | 1996-11-20 | 1997-11-20 | Kloning og karakterisering af napsin, en asparaginprotease |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US6225103B1 (https=) |
| EP (1) | EP0948630B1 (https=) |
| JP (2) | JP2001503643A (https=) |
| AT (1) | ATE393227T1 (https=) |
| AU (1) | AU734220B2 (https=) |
| CA (1) | CA2271294A1 (https=) |
| DE (1) | DE69738653T2 (https=) |
| DK (1) | DK0948630T3 (https=) |
| ES (1) | ES2308791T3 (https=) |
| PT (1) | PT948630E (https=) |
| WO (1) | WO1998022597A2 (https=) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0897986A3 (en) * | 1997-08-21 | 2000-07-05 | Smithkline Beecham Corporation | Aspartic proteinase 5 (ASP5) |
| US6432690B1 (en) * | 1998-01-16 | 2002-08-13 | Incyte Genomics, Inc. | Human aspartic proteases |
| US6203979B1 (en) | 1998-01-16 | 2001-03-20 | Incyte Pharmaceuticals, Inc. | Human protease molecules |
| US7045333B1 (en) | 1998-01-16 | 2006-05-16 | Incyte Corporation | Human protease molecules |
| US7160679B1 (en) | 1998-05-21 | 2007-01-09 | Diadexus, Inc. | Method of diagnosing, monitoring, and staging lung cancer |
| JP2002520048A (ja) * | 1998-07-16 | 2002-07-09 | インサイト・ファーマスーティカルズ・インコーポレイテッド | ヒト・アスパラギン酸プロテアーゼ |
| EP1939297A1 (en) | 1998-09-24 | 2008-07-02 | Pharmacia & Upjohn Company LLC | Alzheimer's disease secretase |
| US7456007B1 (en) | 1998-12-31 | 2008-11-25 | Elan Pharmaceuticals, Inc. | β-secretase enzyme compositions and methods |
| US7115410B1 (en) * | 1999-02-10 | 2006-10-03 | Elan Pharmaceuticals, Inc. | β-secretase enzyme compositions and methods |
| CN1390232A (zh) | 1999-02-10 | 2003-01-08 | 艾兰制药公司 | 人β分泌酶、抑制剂、其组合物和用途 |
| US7514408B1 (en) | 1999-12-02 | 2009-04-07 | Elan Pharmaceuticals, Inc. | β-secretase enzyme compositions and methods |
| JP2001172200A (ja) * | 1999-12-17 | 2001-06-26 | Meneki Seibutsu Kenkyusho:Kk | ヒトナプシンａに対する抗体およびこれを用いる医薬 |
| DE60124080T2 (de) | 2000-03-23 | 2007-03-01 | Elan Pharmaceuticals, Inc., San Francisco | Verbindungen und verfahren zur behandlung der alzheimerschen krankheit |
| WO2002000891A2 (en) * | 2000-06-26 | 2002-01-03 | Bayer Aktiengesellschaft | Human napsin-like aspartyl protease |
| EP1299352B1 (en) | 2000-06-30 | 2005-12-28 | Elan Pharmaceuticals, Inc. | Compounds to treat alzheimer's disease |
| EP1666452A2 (en) | 2000-06-30 | 2006-06-07 | Elan Pharmaceuticals, Inc. | Compounds to treat Alzheimer's disease |
| US6846813B2 (en) | 2000-06-30 | 2005-01-25 | Pharmacia & Upjohn Company | Compounds to treat alzheimer's disease |
| PE20020276A1 (es) | 2000-06-30 | 2002-04-06 | Elan Pharm Inc | COMPUESTOS DE AMINA SUSTITUIDA COMO INHIBIDORES DE ß-SECRETASA PARA EL TRATAMIENTO DE ALZHEIMER |
| EP1500663A1 (en) * | 2000-09-28 | 2005-01-26 | Eli Lilly And Company | Secreted proteins and their uses |
| US7217556B1 (en) | 2000-12-23 | 2007-05-15 | Pfizer Inc | Crystallization and structure determination of glycosylated human beta secretase, an enzyme implicated in Alzheimer's disease |
| US7806980B2 (en) * | 2000-12-23 | 2010-10-05 | Elan Pharmaceuticals, Inc. | Method for crystallizing human beta secretase in complex with an inhibitor |
| US7601528B1 (en) | 2000-12-23 | 2009-10-13 | Elan Pharmaceuticals, Inc. | Crystallization and structure determination of glycosylated human beta secretase, an enzyme implicated in alzheimer's disease |
| US7384773B1 (en) | 2001-05-10 | 2008-06-10 | Pfizer Inc | Crystal of HIV protease-cleaved human beta secretase and method for crystallization thereof |
| US7524668B1 (en) | 2001-05-10 | 2009-04-28 | Elan Pharmaceuticals, Inc. | Crystal of human beta secretase having monoclinic space group symmetry C2 and methods for crystallization thereof |
| BR0210122A (pt) | 2001-06-01 | 2004-06-15 | Elan Pharm Inc | Composto ou um sal farmaceuticamente aceitável do mesmo, método para o tratamento ou prevenção de uma doença, uso do composto ou de seu sal, e, método para preparar o mesmo |
| CA2450205A1 (en) | 2001-06-13 | 2002-12-19 | Elan Pharmaceuticals, Inc. | Aminediols for the treatment of alzheimer's disease |
| EP1401452A1 (en) | 2001-06-27 | 2004-03-31 | Elan Pharmaceuticals, Inc. | Beta-hydroxyamine derivatives useful in the treatment of alzheimer's disease |
| MXPA04000338A (es) | 2001-07-10 | 2004-07-23 | Upjohn Co | Diamindioles para tratamiento de enfermedad de alzheimer. |
| MXPA04000337A (es) | 2001-07-10 | 2004-07-23 | Upjohn Co | Amindioles para tratamiento de enfermedad de alzheimer. |
| US7186539B1 (en) | 2001-08-31 | 2007-03-06 | Pharmacia & Upjohn Company | Method for refolding enzymes |
| CA2462851A1 (en) | 2001-10-04 | 2003-04-10 | Elan Pharmaceuticals, Inc. | Hydroxypropylamines |
| NZ533107A (en) | 2001-11-08 | 2007-04-27 | Upjohn Co | N, N'-substituted-1,3-diamino-2-hydroxypropane derivatives |
| AU2003213252A1 (en) | 2002-02-21 | 2003-09-09 | Pharmacia And Upjohn Company | Modified bace |
| WO2004058686A1 (en) | 2002-04-30 | 2004-07-15 | Elan Pharmaceuticals, Inc. | Hydroxypropyl amides for the treatment of alzheimer’s disease |
| US7442537B1 (en) | 2002-05-10 | 2008-10-28 | Elan Pharmaceuticals, Inc. | Crystals of unliganded beta secretase and/or beta secretase-like proteins and the use thereof |
| AU2003268023A1 (en) * | 2002-07-23 | 2004-02-09 | Incyte Corporation | Protein modification and maintenance molecules |
| US7351738B2 (en) | 2002-11-27 | 2008-04-01 | Elan Pharmaceuticals, Inc. | Substituted ureas and carbamates |
| UY28280A1 (es) | 2003-04-21 | 2004-11-30 | Pharmacia & Amp | 2- hidroxi - 3- diaminoalcanos de benzamida |
| EP1473362A1 (en) | 2003-05-02 | 2004-11-03 | Elan Pharmaceuticals, Inc. | Glycosylation variants of BACE |
| WO2005044977A2 (en) * | 2003-06-25 | 2005-05-19 | Diadexus, Inc. | Compositions, splice variants and methods relating to lung specific genes and proteins |
| US20050239836A1 (en) | 2004-03-09 | 2005-10-27 | Varghese John | Substituted hydroxyethylamine aspartyl protease inhibitors |
| US20070292346A1 (en) * | 2004-03-25 | 2007-12-20 | Rong Fan | Lng105 Antibody Composition and Methods of Use, and Use of Lng105 to Assess Lung Cancer Risk |
| EP1773756A2 (en) | 2004-07-09 | 2007-04-18 | Elan Pharmaceuticals, Inc. | Oxime derivative substituted hydroxyethylamine aspartyl protease inhibitors |
| WO2007047305A1 (en) | 2005-10-12 | 2007-04-26 | Elan Pharmaceuticals, Inc. | Methods of treating amyloidosis using cyclopropyl derivative aspartyl protease inhibitors |
| CA2687750C (en) | 2007-07-06 | 2016-10-18 | Boehringer Ingelheim International Gmbh | Substituted amino-quinazolinones, medicaments comprising said compound, their use and their method of manufacture |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL107250A (en) * | 1993-10-12 | 2001-08-08 | Yeda Res & Dev | Dna molecules involved in programmed cell death, proteins encoded thereby and methods and compositions using said dna molecules and proteins |
| GB9618966D0 (en) * | 1996-09-11 | 1996-10-23 | Smithkline Beecham Plc | Novel compounds |
| US5776759A (en) * | 1996-09-26 | 1998-07-07 | Incyte Pharmaceuticals, Inc. | Two novel human cathepsin proteins |
-
1997
- 1997-11-20 WO PCT/US1997/021684 patent/WO1998022597A2/en not_active Ceased
- 1997-11-20 DK DK97948546T patent/DK0948630T3/da active
- 1997-11-20 EP EP97948546A patent/EP0948630B1/en not_active Expired - Lifetime
- 1997-11-20 AU AU54592/98A patent/AU734220B2/en not_active Ceased
- 1997-11-20 AT AT97948546T patent/ATE393227T1/de active
- 1997-11-20 CA CA002271294A patent/CA2271294A1/en not_active Abandoned
- 1997-11-20 JP JP52398598A patent/JP2001503643A/ja not_active Withdrawn
- 1997-11-20 US US08/974,691 patent/US6225103B1/en not_active Expired - Lifetime
- 1997-11-20 ES ES97948546T patent/ES2308791T3/es not_active Expired - Lifetime
- 1997-11-20 PT PT97948546T patent/PT948630E/pt unknown
- 1997-11-20 DE DE69738653T patent/DE69738653T2/de not_active Expired - Lifetime
-
2007
- 2007-11-15 JP JP2007297327A patent/JP2008118996A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DE69738653T2 (de) | 2009-06-04 |
| EP0948630A2 (en) | 1999-10-13 |
| PT948630E (pt) | 2008-06-11 |
| WO1998022597A3 (en) | 1998-07-09 |
| US6225103B1 (en) | 2001-05-01 |
| AU734220B2 (en) | 2001-06-07 |
| JP2001503643A (ja) | 2001-03-21 |
| EP0948630B1 (en) | 2008-04-23 |
| JP2008118996A (ja) | 2008-05-29 |
| CA2271294A1 (en) | 1998-05-28 |
| AU5459298A (en) | 1998-06-10 |
| ES2308791T3 (es) | 2008-12-01 |
| ATE393227T1 (de) | 2008-05-15 |
| DE69738653D1 (de) | 2008-06-05 |
| WO1998022597A2 (en) | 1998-05-28 |
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