DE69738653D1 - Klonierung und charakterisierung von napsin, einer aspartat-protease - Google Patents

Klonierung und charakterisierung von napsin, einer aspartat-protease

Info

Publication number
DE69738653D1
DE69738653D1 DE69738653T DE69738653T DE69738653D1 DE 69738653 D1 DE69738653 D1 DE 69738653D1 DE 69738653 T DE69738653 T DE 69738653T DE 69738653 T DE69738653 T DE 69738653T DE 69738653 D1 DE69738653 D1 DE 69738653D1
Authority
DE
Germany
Prior art keywords
napsin
enzyme
protease
cloned
expressed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69738653T
Other languages
English (en)
Other versions
DE69738653T2 (de
Inventor
Gerald Koelsch
Xinli Lin
Jordan J Tang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oklahoma Medical Research Foundation
Original Assignee
Oklahoma Medical Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oklahoma Medical Research Foundation filed Critical Oklahoma Medical Research Foundation
Publication of DE69738653D1 publication Critical patent/DE69738653D1/de
Application granted granted Critical
Publication of DE69738653T2 publication Critical patent/DE69738653T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE69738653T 1996-11-20 1997-11-20 Klonierung und charakterisierung von napsin, einer aspartat-protease Expired - Lifetime DE69738653T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US3119696P 1996-11-20 1996-11-20
US31196P 1996-11-20
US4612697P 1997-05-09 1997-05-09
US46126P 1997-05-09
PCT/US1997/021684 WO1998022597A2 (en) 1996-11-20 1997-11-20 Cloning and characterization of napsin, an aspartic protease

Publications (2)

Publication Number Publication Date
DE69738653D1 true DE69738653D1 (de) 2008-06-05
DE69738653T2 DE69738653T2 (de) 2009-06-04

Family

ID=26706937

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69738653T Expired - Lifetime DE69738653T2 (de) 1996-11-20 1997-11-20 Klonierung und charakterisierung von napsin, einer aspartat-protease

Country Status (11)

Country Link
US (1) US6225103B1 (de)
EP (1) EP0948630B1 (de)
JP (2) JP2001503643A (de)
AT (1) ATE393227T1 (de)
AU (1) AU734220B2 (de)
CA (1) CA2271294A1 (de)
DE (1) DE69738653T2 (de)
DK (1) DK0948630T3 (de)
ES (1) ES2308791T3 (de)
PT (1) PT948630E (de)
WO (1) WO1998022597A2 (de)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0897986A3 (de) * 1997-08-21 2000-07-05 Smithkline Beecham Corporation Aspartat proteinase 5 (ASP5)
US6203979B1 (en) 1998-01-16 2001-03-20 Incyte Pharmaceuticals, Inc. Human protease molecules
US7045333B1 (en) 1998-01-16 2006-05-16 Incyte Corporation Human protease molecules
US6432690B1 (en) 1998-01-16 2002-08-13 Incyte Genomics, Inc. Human aspartic proteases
WO2000004137A1 (en) * 1998-07-16 2000-01-27 Incyte Pharmaceuticals, Inc. Human aspartic proteases
EP1939297A1 (de) 1998-09-24 2008-07-02 Pharmacia & Upjohn Company LLC Alzheimerkrankheit-Sekretase
US7115410B1 (en) 1999-02-10 2006-10-03 Elan Pharmaceuticals, Inc. β-secretase enzyme compositions and methods
US7456007B1 (en) 1998-12-31 2008-11-25 Elan Pharmaceuticals, Inc. β-secretase enzyme compositions and methods
EP1165609A2 (de) 1999-02-10 2002-01-02 Elan Pharmaceuticals, Inc. Menschliches beta-secretase enzym, inhibitoren und ihre zusammensetzungen und verwendungen
US7514408B1 (en) 1999-12-02 2009-04-07 Elan Pharmaceuticals, Inc. β-secretase enzyme compositions and methods
JP2001172200A (ja) * 1999-12-17 2001-06-26 Meneki Seibutsu Kenkyusho:Kk ヒトナプシンａに対する抗体およびこれを用いる医薬
AU2001267548A1 (en) * 2000-06-26 2002-01-08 Bayer Aktiengesellschaft Regulation of human napsin-like aspartyl protease
PE20020276A1 (es) 2000-06-30 2002-04-06 Elan Pharm Inc COMPUESTOS DE AMINA SUSTITUIDA COMO INHIBIDORES DE ß-SECRETASA PARA EL TRATAMIENTO DE ALZHEIMER
EP1666452A2 (de) 2000-06-30 2006-06-07 Elan Pharmaceuticals, Inc. Verbindungen zur Behandlung der Alzheimerischen Krankheit
EP1500663A1 (de) * 2000-09-28 2005-01-26 Eli Lilly And Company Sekretierte proteine und ihre Verwendungen
US7806980B2 (en) * 2000-12-23 2010-10-05 Elan Pharmaceuticals, Inc. Method for crystallizing human beta secretase in complex with an inhibitor
US7601528B1 (en) 2000-12-23 2009-10-13 Elan Pharmaceuticals, Inc. Crystallization and structure determination of glycosylated human beta secretase, an enzyme implicated in alzheimer's disease
US7217556B1 (en) 2000-12-23 2007-05-15 Pfizer Inc Crystallization and structure determination of glycosylated human beta secretase, an enzyme implicated in Alzheimer's disease
US7384773B1 (en) * 2001-05-10 2008-06-10 Pfizer Inc Crystal of HIV protease-cleaved human beta secretase and method for crystallization thereof
US7524668B1 (en) 2001-05-10 2009-04-28 Elan Pharmaceuticals, Inc. Crystal of human beta secretase having monoclinic space group symmetry C2 and methods for crystallization thereof
EP1395551B1 (de) 2001-06-01 2008-05-21 Elan Pharmaceuticals, Inc. Hydroxyalkylaminderivate als beta-secretase-inhibitoren und deren verwendung zur behandlung der alzheimerschen krankheit oder ähnlicher krankheiten
JP2004532894A (ja) 2001-06-13 2004-10-28 イーラン　ファーマスーティカルズ、インコーポレイテッド アルツハイマー病を治療するためのアミンジオール
US7053109B2 (en) 2001-07-10 2006-05-30 Pharmacia & Upjohn Company Aminediols for the treatment of Alzheimer's disease
BR0211118A (pt) 2001-07-10 2004-10-26 Elan Pharm Inc Composto, métodos para o tratamento ou prevenção de doenças e para fabricar um composto, intermediário, e, uso de um composto ou sal
US7186539B1 (en) 2001-08-31 2007-03-06 Pharmacia & Upjohn Company Method for refolding enzymes
CA2462851A1 (en) 2001-10-04 2003-04-10 Elan Pharmaceuticals, Inc. Hydroxypropylamines
NZ533107A (en) 2001-11-08 2007-04-27 Upjohn Co N, N'-substituted-1,3-diamino-2-hydroxypropane derivatives
CA2477243A1 (en) 2002-02-21 2003-09-04 Pharmacia & Upjohn Company Modified bace
AU2003303141A1 (en) 2002-04-30 2004-07-22 Elan Pharmaceuticals, Inc. Hydroxypropyl amides for the treatment of alzheimer's disease
US7442537B1 (en) 2002-05-10 2008-10-28 Elan Pharmaceuticals, Inc. Crystals of unliganded beta secretase and/or beta secretase-like proteins and the use thereof
AU2003268023A1 (en) * 2002-07-23 2004-02-09 Incyte Corporation Protein modification and maintenance molecules
BR0316629A (pt) 2002-11-27 2005-10-11 Elan Pharm Inc Uréias e carbamatos substituìdos
AR044044A1 (es) 2003-04-21 2005-08-24 Elan Pharm Inc 2-hidroxi-3-diaminoalcanos de benzamida
WO2004099402A1 (en) 2003-05-02 2004-11-18 Elan Pharmaceuticals, Inc. Glycosylation variants of bace
WO2005044977A2 (en) * 2003-06-25 2005-05-19 Diadexus, Inc. Compositions, splice variants and methods relating to lung specific genes and proteins
CA2558034A1 (en) 2004-03-09 2005-09-22 Elan Pharmaceuticals, Inc. Substituted hydroxyethylamine aspartyl protease inhibitors
US20070292346A1 (en) * 2004-03-25 2007-12-20 Rong Fan Lng105 Antibody Composition and Methods of Use, and Use of Lng105 to Assess Lung Cancer Risk
CA2573138A1 (en) 2004-07-09 2006-01-26 Elan Pharmaceuticals Inc. Oxime derivative substituted hydroxyethylamine aspartyl protease inhibitors
WO2007047305A1 (en) 2005-10-12 2007-04-26 Elan Pharmaceuticals, Inc. Methods of treating amyloidosis using cyclopropyl derivative aspartyl protease inhibitors
JP5450407B2 (ja) 2007-07-06 2014-03-26 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング 置換アミノ−キナゾリノン、前記化合物を含む薬物、それらの使用及び製造方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL107250A (en) * 1993-10-12 2001-08-08 Yeda Res & Dev Dna molecules involved in programmed cell death, proteins encoded thereby and methods and compositions using said dna molecules and proteins
GB9618966D0 (en) * 1996-09-11 1996-10-23 Smithkline Beecham Plc Novel compounds
US5776759A (en) * 1996-09-26 1998-07-07 Incyte Pharmaceuticals, Inc. Two novel human cathepsin proteins

Also Published As

Publication number Publication date
JP2008118996A (ja) 2008-05-29
AU5459298A (en) 1998-06-10
DK0948630T3 (da) 2008-08-11
WO1998022597A3 (en) 1998-07-09
WO1998022597A2 (en) 1998-05-28
EP0948630B1 (de) 2008-04-23
JP2001503643A (ja) 2001-03-21
ES2308791T3 (es) 2008-12-01
PT948630E (pt) 2008-06-11
EP0948630A2 (de) 1999-10-13
DE69738653T2 (de) 2009-06-04
US6225103B1 (en) 2001-05-01
ATE393227T1 (de) 2008-05-15
AU734220B2 (en) 2001-06-07
CA2271294A1 (en) 1998-05-28

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