JP2001503643A - アスパラギン酸プロテアーゼの一種であるナプシンのクローニングおよび特徴付け - Google Patents
アスパラギン酸プロテアーゼの一種であるナプシンのクローニングおよび特徴付けInfo
- Publication number
- JP2001503643A JP2001503643A JP52398598A JP52398598A JP2001503643A JP 2001503643 A JP2001503643 A JP 2001503643A JP 52398598 A JP52398598 A JP 52398598A JP 52398598 A JP52398598 A JP 52398598A JP 2001503643 A JP2001503643 A JP 2001503643A
- Authority
- JP
- Japan
- Prior art keywords
- napsin
- human
- tissue
- sequence
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.単離されたナプシン。 2.前記タンパク質がイソ型Aである、請求項1に記載のナプシン。 3.図1A〜1Dのアミノ酸配列を有する、請求項2に記載のナプシン。 4.図1A〜1Dのヌクレオチド配列によってコードされる、請求項2に記載の ナプシン。 5.前記タンパク質がイソ型Bである、請求項1に記載のナプシン。 6.図4A〜4Eのアミノ酸配列を有する、請求項5に記載のナプシン。 7.図4A〜4Eのヌクレオチド配列によってコードされる、請求項5に記載の ナプシン。 8.ナプシンをコードする、単離されたヌクレオチド分子。 9.ナプシンAをコードする、請求項8に記載の分子。 10.図1A〜1Dのヌクレオチド配列によって示される、請求項9に記載の分 子。 11.ナプシンBをコードする、請求項8に記載の分子。 12.図4A〜4Eのヌクレオチド配列によって示される、請求項11に記載の 分子。 13.検出可能な標識で標識された、請求項8に記載の分子、またはナプシンに 固有の少なくとも14ヌクレオチドの一部。 14.ナプシンを単離するための方法であって、組織抽出物において固定化ペプ スタチンに結合したタンパク質を単離する工程を包含する、方法。 15.前記組織が腎臓細胞である、請求項14に記載の方法。 16.組織に存在するナプシンの量または型を検出するための方法であって、該 組織を、ナプシンをコードするDNAもしくはRNAに特異的にハイブリダイズする標 識化ヌクレオチド分子プローブと反応させる工程、または該組織を、ナプシンと 特異的に免疫反応性の標識化抗体と反応させる工程を包含する、方法。 17.前記組織が、ナプシンAおよびナプシンBの両方の発現のレベルについて スクリーニングされる、請求項16に記載の方法。 18.前記組織に存在するナプシンの量または型が、正常なコントロール組織に 存在するナプシンの量または型と比較される、請求項16に記載の方法。 19.ナプシンと特異的に免疫反応性の抗体。 20.前記抗体が、ナプシンAまたはナプシンBのいずれかと免疫反応性である 、請求項19に記載の抗体。
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US3119696P | 1996-11-20 | 1996-11-20 | |
US60/031,196 | 1996-11-20 | ||
US4612697P | 1997-05-09 | 1997-05-09 | |
US60/046,126 | 1997-05-09 | ||
PCT/US1997/021684 WO1998022597A2 (en) | 1996-11-20 | 1997-11-20 | Cloning and characterization of napsin, an aspartic protease |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2007297327A Division JP2008118996A (ja) | 1996-11-20 | 2007-11-15 | アスパラギン酸プロテアーゼの一種であるナプシンのクローニングおよび特徴付け |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2001503643A true JP2001503643A (ja) | 2001-03-21 |
JP2001503643A5 JP2001503643A5 (ja) | 2005-12-02 |
Family
ID=26706937
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP52398598A Withdrawn JP2001503643A (ja) | 1996-11-20 | 1997-11-20 | アスパラギン酸プロテアーゼの一種であるナプシンのクローニングおよび特徴付け |
JP2007297327A Pending JP2008118996A (ja) | 1996-11-20 | 2007-11-15 | アスパラギン酸プロテアーゼの一種であるナプシンのクローニングおよび特徴付け |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2007297327A Pending JP2008118996A (ja) | 1996-11-20 | 2007-11-15 | アスパラギン酸プロテアーゼの一種であるナプシンのクローニングおよび特徴付け |
Country Status (11)
Country | Link |
---|---|
US (1) | US6225103B1 (ja) |
EP (1) | EP0948630B1 (ja) |
JP (2) | JP2001503643A (ja) |
AT (1) | ATE393227T1 (ja) |
AU (1) | AU734220B2 (ja) |
CA (1) | CA2271294A1 (ja) |
DE (1) | DE69738653T2 (ja) |
DK (1) | DK0948630T3 (ja) |
ES (1) | ES2308791T3 (ja) |
PT (1) | PT948630E (ja) |
WO (1) | WO1998022597A2 (ja) |
Families Citing this family (40)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0897986A3 (en) * | 1997-08-21 | 2000-07-05 | Smithkline Beecham Corporation | Aspartic proteinase 5 (ASP5) |
US6203979B1 (en) | 1998-01-16 | 2001-03-20 | Incyte Pharmaceuticals, Inc. | Human protease molecules |
US7045333B1 (en) | 1998-01-16 | 2006-05-16 | Incyte Corporation | Human protease molecules |
US6432690B1 (en) * | 1998-01-16 | 2002-08-13 | Incyte Genomics, Inc. | Human aspartic proteases |
AU5213199A (en) * | 1998-07-16 | 2000-02-07 | Incyte Pharmaceuticals, Inc. | Human aspartic proteases |
EP1939297A1 (en) | 1998-09-24 | 2008-07-02 | Pharmacia & Upjohn Company LLC | Alzheimer's disease secretase |
US7115410B1 (en) * | 1999-02-10 | 2006-10-03 | Elan Pharmaceuticals, Inc. | β-secretase enzyme compositions and methods |
US7456007B1 (en) | 1998-12-31 | 2008-11-25 | Elan Pharmaceuticals, Inc. | β-secretase enzyme compositions and methods |
CN1390232A (zh) | 1999-02-10 | 2003-01-08 | 艾兰制药公司 | 人β分泌酶、抑制剂、其组合物和用途 |
US7514408B1 (en) | 1999-12-02 | 2009-04-07 | Elan Pharmaceuticals, Inc. | β-secretase enzyme compositions and methods |
JP2001172200A (ja) * | 1999-12-17 | 2001-06-26 | Meneki Seibutsu Kenkyusho:Kk | ヒトナプシンaに対する抗体およびこれを用いる医薬 |
AU2001267548A1 (en) * | 2000-06-26 | 2002-01-08 | Bayer Aktiengesellschaft | Regulation of human napsin-like aspartyl protease |
PE20020276A1 (es) | 2000-06-30 | 2002-04-06 | Elan Pharm Inc | COMPUESTOS DE AMINA SUSTITUIDA COMO INHIBIDORES DE ß-SECRETASA PARA EL TRATAMIENTO DE ALZHEIMER |
EP1666452A2 (en) | 2000-06-30 | 2006-06-07 | Elan Pharmaceuticals, Inc. | Compounds to treat Alzheimer's disease |
EP1500663A1 (en) * | 2000-09-28 | 2005-01-26 | Eli Lilly And Company | Secreted proteins and their uses |
US7806980B2 (en) * | 2000-12-23 | 2010-10-05 | Elan Pharmaceuticals, Inc. | Method for crystallizing human beta secretase in complex with an inhibitor |
US7601528B1 (en) | 2000-12-23 | 2009-10-13 | Elan Pharmaceuticals, Inc. | Crystallization and structure determination of glycosylated human beta secretase, an enzyme implicated in alzheimer's disease |
US7217556B1 (en) | 2000-12-23 | 2007-05-15 | Pfizer Inc | Crystallization and structure determination of glycosylated human beta secretase, an enzyme implicated in Alzheimer's disease |
US7384773B1 (en) * | 2001-05-10 | 2008-06-10 | Pfizer Inc | Crystal of HIV protease-cleaved human beta secretase and method for crystallization thereof |
US7524668B1 (en) * | 2001-05-10 | 2009-04-28 | Elan Pharmaceuticals, Inc. | Crystal of human beta secretase having monoclinic space group symmetry C2 and methods for crystallization thereof |
DE60226729D1 (de) | 2001-06-01 | 2008-07-03 | Elan Pharm Inc | Hydroxyalkylaminderivate als beta-secretase-inhibitoren und deren verwendung zur behandlung der alzheimerschen krankheit oder ähnlicher krankheiten |
EP1395251A2 (en) | 2001-06-13 | 2004-03-10 | Elan Pharmaceuticals, Inc. | Aminediols as agents for the treatment of alzheimer's disease |
CA2453451A1 (en) | 2001-07-10 | 2003-01-23 | Elan Pharmaceuticals, Inc. | Aminediols for the treatment of alzheimer's disease |
WO2003006013A1 (en) | 2001-07-10 | 2003-01-23 | Elan Pharmaceuticals, Inc. | Diaminediols for the treatment of alzheimer's disease |
US7186539B1 (en) | 2001-08-31 | 2007-03-06 | Pharmacia & Upjohn Company | Method for refolding enzymes |
CA2462851A1 (en) | 2001-10-04 | 2003-04-10 | Elan Pharmaceuticals, Inc. | Hydroxypropylamines |
EA200400648A1 (ru) | 2001-11-08 | 2005-04-28 | Элан Фармасьютикалз, Инк. | N, n'-замещенные производные 1,3-диамино-2-гидроксипропана |
CA2477243A1 (en) | 2002-02-21 | 2003-09-04 | Pharmacia & Upjohn Company | Modified bace |
AU2003303141A1 (en) | 2002-04-30 | 2004-07-22 | Elan Pharmaceuticals, Inc. | Hydroxypropyl amides for the treatment of alzheimer's disease |
US7442537B1 (en) | 2002-05-10 | 2008-10-28 | Elan Pharmaceuticals, Inc. | Crystals of unliganded beta secretase and/or beta secretase-like proteins and the use thereof |
WO2004009797A2 (en) * | 2002-07-23 | 2004-01-29 | Incyte Corporation | Protein modification and maintenance molecules |
AU2003293155A1 (en) | 2002-11-27 | 2004-06-23 | Elan Pharmaceutical, Inc. | Substituted ureas and carbamates |
TW200512195A (en) | 2003-04-21 | 2005-04-01 | Elan Pharm Inc | Benzamide 2-hydroxy-3-diaminoalkanes |
JP4739192B2 (ja) | 2003-05-02 | 2011-08-03 | イーラン ファーマスーティカルズ、インコーポレイテッド | Baceのグリコシル化変異体 |
WO2005044977A2 (en) * | 2003-06-25 | 2005-05-19 | Diadexus, Inc. | Compositions, splice variants and methods relating to lung specific genes and proteins |
WO2005087751A2 (en) | 2004-03-09 | 2005-09-22 | Elan Pharmaceuticals, Inc. | Substituted hydroxyethylamine aspartyl protease inhibitors |
WO2005095454A1 (en) * | 2004-03-25 | 2005-10-13 | Diadexus, Inc. | Lng105 antibody composition and methods of use, and use of lng105 to assess lung cancer risk |
US7385085B2 (en) | 2004-07-09 | 2008-06-10 | Elan Pharmaceuticals, Inc. | Oxime derivative substituted hydroxyethylamine aspartyl protease inhibitors |
WO2007047305A1 (en) | 2005-10-12 | 2007-04-26 | Elan Pharmaceuticals, Inc. | Methods of treating amyloidosis using cyclopropyl derivative aspartyl protease inhibitors |
EP2164834A2 (en) | 2007-07-06 | 2010-03-24 | Boehringer Ingelheim International GmbH | Substituted amino-quinazolinones, medicaments comprising said compound, their use and their method of manufacture |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL107250A (en) * | 1993-10-12 | 2001-08-08 | Yeda Res & Dev | Dna molecules involved in programmed cell death, proteins encoded thereby and methods and compositions using said dna molecules and proteins |
GB9618966D0 (en) * | 1996-09-11 | 1996-10-23 | Smithkline Beecham Plc | Novel compounds |
US5776759A (en) * | 1996-09-26 | 1998-07-07 | Incyte Pharmaceuticals, Inc. | Two novel human cathepsin proteins |
-
1997
- 1997-11-20 PT PT97948546T patent/PT948630E/pt unknown
- 1997-11-20 ES ES97948546T patent/ES2308791T3/es not_active Expired - Lifetime
- 1997-11-20 DE DE69738653T patent/DE69738653T2/de not_active Expired - Lifetime
- 1997-11-20 CA CA002271294A patent/CA2271294A1/en not_active Abandoned
- 1997-11-20 AU AU54592/98A patent/AU734220B2/en not_active Ceased
- 1997-11-20 US US08/974,691 patent/US6225103B1/en not_active Expired - Lifetime
- 1997-11-20 AT AT97948546T patent/ATE393227T1/de active
- 1997-11-20 EP EP97948546A patent/EP0948630B1/en not_active Expired - Lifetime
- 1997-11-20 WO PCT/US1997/021684 patent/WO1998022597A2/en active IP Right Grant
- 1997-11-20 DK DK97948546T patent/DK0948630T3/da active
- 1997-11-20 JP JP52398598A patent/JP2001503643A/ja not_active Withdrawn
-
2007
- 2007-11-15 JP JP2007297327A patent/JP2008118996A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
CA2271294A1 (en) | 1998-05-28 |
EP0948630B1 (en) | 2008-04-23 |
PT948630E (pt) | 2008-06-11 |
AU734220B2 (en) | 2001-06-07 |
JP2008118996A (ja) | 2008-05-29 |
ES2308791T3 (es) | 2008-12-01 |
ATE393227T1 (de) | 2008-05-15 |
DE69738653D1 (de) | 2008-06-05 |
WO1998022597A2 (en) | 1998-05-28 |
US6225103B1 (en) | 2001-05-01 |
WO1998022597A3 (en) | 1998-07-09 |
AU5459298A (en) | 1998-06-10 |
DK0948630T3 (da) | 2008-08-11 |
DE69738653T2 (de) | 2009-06-04 |
EP0948630A2 (en) | 1999-10-13 |
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