DK0824598T3 - Fremgangsmåde til specifik kobling af kappen på ende-5' af et mRNA fragment og fremstilling af komplet mRNA og cDNA - Google Patents

Fremgangsmåde til specifik kobling af kappen på ende-5' af et mRNA fragment og fremstilling af komplet mRNA og cDNA

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Publication number
DK0824598T3
DK0824598T3 DK96914259T DK96914259T DK0824598T3 DK 0824598 T3 DK0824598 T3 DK 0824598T3 DK 96914259 T DK96914259 T DK 96914259T DK 96914259 T DK96914259 T DK 96914259T DK 0824598 T3 DK0824598 T3 DK 0824598T3
Authority
DK
Denmark
Prior art keywords
mrna
pct
preparation
specific coupling
cdna
Prior art date
Application number
DK96914259T
Other languages
Danish (da)
English (en)
Inventor
Irena Nicolaevna Merenkova
Milne Edwards Jean-Bapti Dumas
Original Assignee
Genset Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR9505221A external-priority patent/FR2733765B1/fr
Application filed by Genset Sa filed Critical Genset Sa
Application granted granted Critical
Publication of DK0824598T3 publication Critical patent/DK0824598T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cosmetics (AREA)
  • Mechanical Coupling Of Light Guides (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Saccharide Compounds (AREA)
DK96914259T 1995-05-02 1996-04-29 Fremgangsmåde til specifik kobling af kappen på ende-5' af et mRNA fragment og fremstilling af komplet mRNA og cDNA DK0824598T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9505221A FR2733765B1 (fr) 1995-05-02 1995-05-02 Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm
FR9509467A FR2733762B1 (fr) 1995-05-02 1995-08-03 Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet
PCT/FR1996/000651 WO1996034981A2 (fr) 1995-05-02 1996-04-29 METHODE DE COUPLAGE SPECIFIQUE DE LA COIFFE DE L'EXTREMITE 5' D'UN FRAGMENT D'ARNm ET PREPARATION D'ARNm ET D'ADNc COMPLET

Publications (1)

Publication Number Publication Date
DK0824598T3 true DK0824598T3 (da) 2003-03-10

Family

ID=26231928

Family Applications (1)

Application Number Title Priority Date Filing Date
DK96914259T DK0824598T3 (da) 1995-05-02 1996-04-29 Fremgangsmåde til specifik kobling af kappen på ende-5' af et mRNA fragment og fremstilling af komplet mRNA og cDNA

Country Status (12)

Country Link
US (1) US6022715A (de)
EP (1) EP0824598B1 (de)
JP (1) JPH11510364A (de)
AT (1) ATE227346T1 (de)
AU (1) AU719217B2 (de)
CA (1) CA2220045C (de)
DE (1) DE69624695T2 (de)
DK (1) DK0824598T3 (de)
ES (1) ES2186778T3 (de)
FR (1) FR2733762B1 (de)
PT (1) PT824598E (de)
WO (1) WO1996034981A2 (de)

Families Citing this family (90)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
JP3441899B2 (ja) * 1996-11-01 2003-09-02 理化学研究所 完全長ｃＤＮＡライブラリーの作成方法
JP4447664B2 (ja) 1997-06-12 2010-04-07 スローン―ケタリング・インスティテュート・フォー・キャンサー・リサーチ ワクシニアトポイソメラーゼにより触媒されるｒｎａ鎖に対するｄｎａ鎖の共有結合
JP2001512013A (ja) * 1997-08-01 2001-08-21 ジェンセット 前立腺に発現される分泌タンパク質の５’ｅｓｔ
WO1999006439A2 (en) * 1997-08-01 1999-02-11 Genset 5' ESTs FOR SECRETED PROTEINS EXPRESSED IN ENDODERM
US6222029B1 (en) * 1997-08-01 2001-04-24 Genset 5′ ESTs for secreted proteins expressed in brain
PT1375514E (pt) * 1997-08-01 2006-10-31 Serono Genetics Inst Sa Ests 5' para proteinas secretadas expressas em varios tecidos
US7393663B2 (en) 1997-08-01 2008-07-01 Serono Genetics Institute S.A. Expressed sequence tags and encoded human proteins
ES2267192T3 (es) * 1997-08-01 2007-03-01 Serono Genetics Institute S.A. Ests 5' para proteinas secretadas no especificas de tejido.
ES2359401T3 (es) * 1997-08-01 2011-05-23 Merck Serono Biodevelopment ESTs 5¿PARA PROTEÍNAS SECRETADAS, EXPRESADAS EN DIVERSOS TEJIDOS.
CA2297109A1 (en) * 1997-08-01 1999-02-11 Genset 5' ests for secreted proteins expressed in muscle and other mesodermal tissues
CA2296809A1 (en) * 1997-08-01 1999-02-11 Genset 5' ests for secreted proteins identified from brain tissues
FR2767135B1 (fr) 1997-08-06 2002-07-12 Genset Sa Recepteur complexe lsr, activite, clonage, et application au diagnostic, a la prevention et/ou au traitement de d'obesite et des risques ou complications associes
CA2306777A1 (en) * 1997-10-22 1999-04-29 Helix Research Institute Method for screening full-length cdna clones
US6548633B1 (en) 1998-12-22 2003-04-15 Genset, S.A. Complementary DNA's encoding proteins with signal peptides
WO1999040189A2 (en) * 1998-02-09 1999-08-12 Genset Cdnas encoding secreted proteins
EP1029045A2 (de) * 1997-11-13 2000-08-23 Genset VERLÄNGERTE cDNAs, DIE FÜR SEKRETIERTE PROTIEINE KODIEREN
EP1903111A3 (de) * 1997-11-13 2008-07-02 Serono Genetics Institute S.A. Erweiterte cDNA für sekretierte Proteine
US6573068B1 (en) 1997-11-13 2003-06-03 Genset, S. A. Claudin-50 protein
US20060286639A1 (en) * 1997-11-13 2006-12-21 Serono Genetics Institute S.A. Complementary DNAs encoding proteins with signal peptides
ATE440951T1 (de) * 1997-12-17 2009-09-15 Serono Genetics Inst Sa Verlängerte cdns, die für sekretierte proteine kodieren
US7223727B2 (en) 1998-04-09 2007-05-29 Serono Genetics Institute S.A. GSSP4 polynucleotides and polypeptides and uses thereof
US6783961B1 (en) 1999-02-26 2004-08-31 Genset S.A. Expressed sequence tags and encoded human proteins
JP2002511259A (ja) 1998-04-09 2002-04-16 ジェンセット ５’ｅｓｔおよびコードされるヒトタンパク質
ATE397064T1 (de) 1998-11-04 2008-06-15 Serono Genetics Inst Sa Genomische und vollständige cdna sequenzen von menschlichem adipozyten spezifischem apm1 und biallelische marker davon
US6455280B1 (en) 1998-12-22 2002-09-24 Genset S.A. Methods and compositions for inhibiting neoplastic cell growth
ES2323445T3 (es) 1999-02-10 2009-07-16 Serono Genetics Institute S.A. Marcadores polimorficos del gen lsr.
EP1586645A3 (de) 1999-02-25 2006-02-22 Ceres Incorporated DNA-Fragmente mit bestimmter Sequenz und die dadurch kodierte Polypeptide
EP1163357A4 (de) * 1999-03-19 2004-11-10 Inst Genetics Llc PRIMER-GEBUNDENE VEKTOREN-VERLäNGERUNG (PAVE): EINE AUF DAS 5'-ENDE GERICHTETE CDNS KLONIERUNGSSTRATEGIE
JP2002539784A (ja) * 1999-03-19 2002-11-26 インヴィトロゲン・コーポレーション 大腸菌トポイソメラーゼｉｉｉ及びその相同体を使用して全長核酸配列を得る方法
US7485715B2 (en) 1999-06-18 2009-02-03 Ceres, Inc. Sequence-determined DNA encoding AP2 domain polypeptides
US7479555B2 (en) 1999-07-21 2009-01-20 Ceres, Inc. Polynucleotides having a nucleotide sequence that encodes a polypeptide having MOV34 family activity
CA2311201A1 (en) * 1999-08-05 2001-02-05 Genset S.A. Ests and encoded human proteins
US20020058617A1 (en) 2000-01-14 2002-05-16 Joachim Fruebis OBG3 globular head and uses thereof for decreasing body mass
US6566332B2 (en) 2000-01-14 2003-05-20 Genset S.A. OBG3 globular head and uses thereof for decreasing body mass
US6989367B2 (en) 2000-01-14 2006-01-24 Genset S.A. OBG3 globular head and uses thereof
US7338787B2 (en) 2000-01-14 2008-03-04 Serono Genetics Institute S.A. Nucleic acids encoding OBG3 globular head and uses thereof
EP1261724A4 (de) 2000-02-25 2004-08-18 Invitrogen Corp Topoisomerase linker-vermittelte amplifizierungsverfahren
US6387624B1 (en) * 2000-04-14 2002-05-14 Incyte Pharmaceuticals, Inc. Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
WO2002016594A2 (en) 2000-08-21 2002-02-28 Invitrogen Corporation Methods and reagents for molecular cloning
US6875907B2 (en) 2000-09-13 2005-04-05 Pioneer Hi-Bred International, Inc. Antimicrobial peptides and methods of use
US20060008817A1 (en) 2000-12-08 2006-01-12 Invitrogen Corporation Methods and compositions for generating recombinant nucleic acid molecules
NZ526194A (en) 2000-12-08 2004-10-29 Invitrogen Corp Methods of covalently linking, in one or both strands, two or more double stranded nucleotide sequences using one or more topoisomerases
MXPA04002817A (es) 2001-09-27 2004-07-05 Pioneer Hi Bred Int Polinucleotidos de fitato y metodos de uso.
WO2003087313A2 (en) 2002-04-08 2003-10-23 Pioneer Hi-Bred International, Inc. Enhanced silk exsertion under stress
AU2003238702A1 (en) * 2002-06-12 2003-12-31 Kabushiki Kaisha Dnaform Method of utilizing the 5'end of transcribed nucleic acid regions for cloning and analysis
JP4517114B2 (ja) * 2003-03-28 2010-08-04 国立障害者リハビリテーションセンター総長 ｃＤＮＡ合成方法
EP1697534B1 (de) 2003-12-01 2010-06-02 Life Technologies Corporation Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
GB0417887D0 (en) 2004-08-11 2004-09-15 Ares Trading Sa Protein
US8206924B2 (en) * 2006-09-11 2012-06-26 Osaka University Trace mRNA amplification method and use thereof
CN101932712B (zh) 2007-11-20 2014-05-14 先锋国际良种公司 用于改善植物应激耐性的玉米乙烯信号转导基因及其调节
US20100035249A1 (en) * 2008-08-05 2010-02-11 Kabushiki Kaisha Dnaform Rna sequencing and analysis using solid support
WO2010101818A1 (en) 2009-03-02 2010-09-10 Pioneer Hi-Bred International, Inc. Nac transcriptional activators involved in abiotic stress tolerance
WO2011017492A2 (en) 2009-08-05 2011-02-10 Pioneer Hi-Bred International, Inc. Novel eto1 genes and use of same for reduced ethylene and improved stress tolerance in plants
CA2807552A1 (en) 2010-08-06 2012-02-09 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
CN104531812A (zh) 2010-10-01 2015-04-22 现代治疗公司 设计核酸及其使用方法
WO2012135805A2 (en) 2011-03-31 2012-10-04 modeRNA Therapeutics Delivery and formulation of engineered nucleic acids
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
RU2707251C2 (ru) 2011-10-03 2019-11-25 Модерна Терапьютикс, Инк. Модифицированные нуклеозиды, нуклеотиды и нуклеиновые кислоты и их применение
CN103906835A (zh) 2011-10-25 2014-07-02 先锋国际良种公司 改变植物细胞壁组成以改善生物燃料生产和青贮饲料可消化性的方法
KR20140102759A (ko) 2011-12-16 2014-08-22 모더나 세라퓨틱스, 인코포레이티드 변형된 뉴클레오사이드, 뉴클레오타이드 및 핵산 조성물
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
EP2833892A4 (de) 2012-04-02 2016-07-20 Moderna Therapeutics Inc Modifizierte polynukleotide zur herstellung von in der onkologie nützlichen proteinen und peptiden
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9878056B2 (en) 2012-04-02 2018-01-30 Modernatx, Inc. Modified polynucleotides for the production of cosmetic proteins and peptides
EP2684954A1 (de) 2012-07-10 2014-01-15 Lexogen GmbH 5'-schutzabhängige Verstärkung
US9422602B2 (en) * 2012-08-15 2016-08-23 Bio-Rad Laboratories, Inc. Methods and compositions for determining nucleic acid degradation
JP6144355B2 (ja) 2012-11-26 2017-06-07 モデルナティエックス インコーポレイテッドＭｏｄｅｒｎａＴＸ，Ｉｎｃ． 化学修飾ｍＲＮＡ
WO2014152027A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Manufacturing methods for production of rna transcripts
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
WO2014144767A1 (en) 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
WO2014152030A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Removal of dna fragments in mrna production process
US11377470B2 (en) 2013-03-15 2022-07-05 Modernatx, Inc. Ribonucleic acid purification
BR112015023703A2 (pt) 2013-03-15 2017-07-18 Pioneer Hi Bred Int modulação de expressão de deaminase de acc
EP3971287A1 (de) 2013-07-11 2022-03-23 ModernaTX, Inc. Zusammensetzungen mit synthetischen polynukleotiden zur codierung von crispr-verwandten proteinen und synthetischen sgrnas und verfahren zur verwendung
WO2015048744A2 (en) 2013-09-30 2015-04-02 Moderna Therapeutics, Inc. Polynucleotides encoding immune modulating polypeptides
WO2015051169A2 (en) 2013-10-02 2015-04-09 Moderna Therapeutics, Inc. Polynucleotide molecules and uses thereof
US10323076B2 (en) 2013-10-03 2019-06-18 Modernatx, Inc. Polynucleotides encoding low density lipoprotein receptor
US11479766B2 (en) * 2013-12-05 2022-10-25 New England Biolabs, Inc. Methods for labeling a population of RNA molecules
AU2015289656A1 (en) 2014-07-16 2017-02-16 Modernatx, Inc. Circular polynucleotides
WO2017049286A1 (en) 2015-09-17 2017-03-23 Moderna Therapeutics, Inc. Polynucleotides containing a morpholino linker
ES2914225T3 (es) 2015-10-16 2022-06-08 Modernatx Inc Análogos de cap de ARNm con enlace de fosfato modificado
US11866754B2 (en) 2015-10-16 2024-01-09 Modernatx, Inc. Trinucleotide mRNA cap analogs
EP3409773B1 (de) 2016-01-27 2020-07-29 Kabushiki Kaisha DNAFORM Verfahren zur decodierung einer basensequenz von nukleinsäuren entsprechend einer terminalen rns-region und verfahren zur analyse eines dns-elements
CN109937253B (zh) 2016-09-14 2023-06-30 摩登纳特斯有限公司 高纯度rna组合物及其制备方法
WO2019036683A1 (en) 2017-08-18 2019-02-21 Modernatx, Inc. ANALYTICAL METHODS BY HPLC
MA49922A (fr) 2017-08-18 2021-06-02 Modernatx Inc Procédés pour analyse par clhp
WO2023127774A1 (ja) * 2021-12-28 2023-07-06 株式会社ダナフォーム 遺伝子包含体の検出方法及び検出用キット
WO2023237703A1 (en) 2022-06-09 2023-12-14 Vestlandets Innovasjonsselskap As Method of characterizing capped rna

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4661450A (en) * 1983-05-03 1987-04-28 Molecular Genetics Research And Development Limited Partnership Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides
US4710566A (en) * 1984-12-27 1987-12-01 Yale University Purification of the messenger RNA cap-binding protein
US5273879A (en) * 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US5219989A (en) * 1988-12-13 1993-06-15 Mcgill University Bifunctional protein for the isolation of capped mRNA
CA2005252C (en) * 1988-12-13 2001-07-17 Nahum Sonenberg Isolation of full-length cdna clones and capped mrna
US5324829A (en) * 1988-12-16 1994-06-28 Ortho Diagnostic Systems, Inc. High specific activity nucleic acid probes having target recognition and signal generating moieties
JP3315439B2 (ja) * 1992-07-21 2002-08-19 住友電気工業株式会社 細胞特異的ｃＤＮＡの分離法及びライブラリー作製法
JP3337748B2 (ja) * 1992-09-25 2002-10-21 財団法人神奈川科学技術アカデミー 完全長ｃＤＮＡの合成方法、その中間体の製造方法及び完全長ｃＤＮＡを含む組換えベクターの製造方法

Also Published As

Publication number Publication date
EP0824598B1 (de) 2002-11-06
US6022715A (en) 2000-02-08
FR2733762A1 (fr) 1996-11-08
WO1996034981A2 (fr) 1996-11-07
PT824598E (pt) 2003-03-31
FR2733762B1 (fr) 1997-08-01
ES2186778T3 (es) 2003-05-16
EP0824598A2 (de) 1998-02-25
ATE227346T1 (de) 2002-11-15
CA2220045A1 (fr) 1996-11-07
WO1996034981A3 (fr) 1997-01-03
AU719217B2 (en) 2000-05-04
AU5982996A (en) 1996-11-21
CA2220045C (fr) 2001-10-30
DE69624695D1 (de) 2002-12-12
JPH11510364A (ja) 1999-09-14
DE69624695T2 (de) 2003-07-31

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