DE69624695D1 - VERFAHREN ZUR SPEZIFISCHEN KOPPLUNG EINER SCHUTZGRUPPE AN DAS 5'-ENDE EINES mRNA-FRAGMENTES UND DIE HERSTELLUNG VON mRNA UND VOLLSTÄNDIGER cDNA - Google Patents
VERFAHREN ZUR SPEZIFISCHEN KOPPLUNG EINER SCHUTZGRUPPE AN DAS 5'-ENDE EINES mRNA-FRAGMENTES UND DIE HERSTELLUNG VON mRNA UND VOLLSTÄNDIGER cDNAInfo
- Publication number
- DE69624695D1 DE69624695D1 DE69624695T DE69624695T DE69624695D1 DE 69624695 D1 DE69624695 D1 DE 69624695D1 DE 69624695 T DE69624695 T DE 69624695T DE 69624695 T DE69624695 T DE 69624695T DE 69624695 D1 DE69624695 D1 DE 69624695D1
- Authority
- DE
- Germany
- Prior art keywords
- mrna
- pct
- specific coupling
- production
- fragment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 108020004999 messenger RNA Proteins 0.000 title abstract 7
- 238000000034 method Methods 0.000 title abstract 5
- 239000002299 complementary DNA Substances 0.000 title abstract 4
- 230000008878 coupling Effects 0.000 title abstract 2
- 238000010168 coupling process Methods 0.000 title abstract 2
- 238000005859 coupling reaction Methods 0.000 title abstract 2
- 239000012634 fragment Substances 0.000 title abstract 2
- 230000001681 protective effect Effects 0.000 title 1
- 125000003277 amino group Chemical group 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Cosmetics (AREA)
- Mechanical Coupling Of Light Guides (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9505221A FR2733765B1 (fr) | 1995-05-02 | 1995-05-02 | Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm |
FR9509467A FR2733762B1 (fr) | 1995-05-02 | 1995-08-03 | Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet |
PCT/FR1996/000651 WO1996034981A2 (fr) | 1995-05-02 | 1996-04-29 | METHODE DE COUPLAGE SPECIFIQUE DE LA COIFFE DE L'EXTREMITE 5' D'UN FRAGMENT D'ARNm ET PREPARATION D'ARNm ET D'ADNc COMPLET |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69624695D1 true DE69624695D1 (de) | 2002-12-12 |
DE69624695T2 DE69624695T2 (de) | 2003-07-31 |
Family
ID=26231928
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69624695T Expired - Fee Related DE69624695T2 (de) | 1995-05-02 | 1996-04-29 | VERFAHREN ZUR SPEZIFISCHEN KOPPLUNG EINER SCHUTZGRUPPE AN DAS 5'-ENDE EINES mRNA-FRAGMENTES UND DIE HERSTELLUNG VON mRNA UND VOLLSTÄNDIGER cDNA |
Country Status (12)
Country | Link |
---|---|
US (1) | US6022715A (de) |
EP (1) | EP0824598B1 (de) |
JP (1) | JPH11510364A (de) |
AT (1) | ATE227346T1 (de) |
AU (1) | AU719217B2 (de) |
CA (1) | CA2220045C (de) |
DE (1) | DE69624695T2 (de) |
DK (1) | DK0824598T3 (de) |
ES (1) | ES2186778T3 (de) |
FR (1) | FR2733762B1 (de) |
PT (1) | PT824598E (de) |
WO (1) | WO1996034981A2 (de) |
Families Citing this family (90)
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US5766891A (en) | 1994-12-19 | 1998-06-16 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
JP3441899B2 (ja) * | 1996-11-01 | 2003-09-02 | 理化学研究所 | 完全長cDNAライブラリーの作成方法 |
JP4447664B2 (ja) | 1997-06-12 | 2010-04-07 | スローン―ケタリング・インスティテュート・フォー・キャンサー・リサーチ | ワクシニアトポイソメラーゼにより触媒されるrna鎖に対するdna鎖の共有結合 |
JP2001512013A (ja) * | 1997-08-01 | 2001-08-21 | ジェンセット | 前立腺に発現される分泌タンパク質の5’est |
WO1999006439A2 (en) * | 1997-08-01 | 1999-02-11 | Genset | 5' ESTs FOR SECRETED PROTEINS EXPRESSED IN ENDODERM |
US6222029B1 (en) * | 1997-08-01 | 2001-04-24 | Genset | 5′ ESTs for secreted proteins expressed in brain |
PT1375514E (pt) * | 1997-08-01 | 2006-10-31 | Serono Genetics Inst Sa | Ests 5' para proteinas secretadas expressas em varios tecidos |
US7393663B2 (en) | 1997-08-01 | 2008-07-01 | Serono Genetics Institute S.A. | Expressed sequence tags and encoded human proteins |
ES2267192T3 (es) * | 1997-08-01 | 2007-03-01 | Serono Genetics Institute S.A. | Ests 5' para proteinas secretadas no especificas de tejido. |
ES2359401T3 (es) * | 1997-08-01 | 2011-05-23 | Merck Serono Biodevelopment | ESTs 5¿PARA PROTEÍNAS SECRETADAS, EXPRESADAS EN DIVERSOS TEJIDOS. |
CA2297109A1 (en) * | 1997-08-01 | 1999-02-11 | Genset | 5' ests for secreted proteins expressed in muscle and other mesodermal tissues |
CA2296809A1 (en) * | 1997-08-01 | 1999-02-11 | Genset | 5' ests for secreted proteins identified from brain tissues |
FR2767135B1 (fr) | 1997-08-06 | 2002-07-12 | Genset Sa | Recepteur complexe lsr, activite, clonage, et application au diagnostic, a la prevention et/ou au traitement de d'obesite et des risques ou complications associes |
CA2306777A1 (en) * | 1997-10-22 | 1999-04-29 | Helix Research Institute | Method for screening full-length cdna clones |
US6548633B1 (en) | 1998-12-22 | 2003-04-15 | Genset, S.A. | Complementary DNA's encoding proteins with signal peptides |
WO1999040189A2 (en) * | 1998-02-09 | 1999-08-12 | Genset | Cdnas encoding secreted proteins |
EP1029045A2 (de) * | 1997-11-13 | 2000-08-23 | Genset | VERLÄNGERTE cDNAs, DIE FÜR SEKRETIERTE PROTIEINE KODIEREN |
EP1903111A3 (de) * | 1997-11-13 | 2008-07-02 | Serono Genetics Institute S.A. | Erweiterte cDNA für sekretierte Proteine |
US6573068B1 (en) | 1997-11-13 | 2003-06-03 | Genset, S. A. | Claudin-50 protein |
US20060286639A1 (en) * | 1997-11-13 | 2006-12-21 | Serono Genetics Institute S.A. | Complementary DNAs encoding proteins with signal peptides |
ATE440951T1 (de) * | 1997-12-17 | 2009-09-15 | Serono Genetics Inst Sa | Verlängerte cdns, die für sekretierte proteine kodieren |
US7223727B2 (en) | 1998-04-09 | 2007-05-29 | Serono Genetics Institute S.A. | GSSP4 polynucleotides and polypeptides and uses thereof |
US6783961B1 (en) | 1999-02-26 | 2004-08-31 | Genset S.A. | Expressed sequence tags and encoded human proteins |
JP2002511259A (ja) | 1998-04-09 | 2002-04-16 | ジェンセット | 5’estおよびコードされるヒトタンパク質 |
ATE397064T1 (de) | 1998-11-04 | 2008-06-15 | Serono Genetics Inst Sa | Genomische und vollständige cdna sequenzen von menschlichem adipozyten spezifischem apm1 und biallelische marker davon |
US6455280B1 (en) | 1998-12-22 | 2002-09-24 | Genset S.A. | Methods and compositions for inhibiting neoplastic cell growth |
ES2323445T3 (es) | 1999-02-10 | 2009-07-16 | Serono Genetics Institute S.A. | Marcadores polimorficos del gen lsr. |
EP1586645A3 (de) | 1999-02-25 | 2006-02-22 | Ceres Incorporated | DNA-Fragmente mit bestimmter Sequenz und die dadurch kodierte Polypeptide |
EP1163357A4 (de) * | 1999-03-19 | 2004-11-10 | Inst Genetics Llc | PRIMER-GEBUNDENE VEKTOREN-VERLäNGERUNG (PAVE): EINE AUF DAS 5'-ENDE GERICHTETE CDNS KLONIERUNGSSTRATEGIE |
JP2002539784A (ja) * | 1999-03-19 | 2002-11-26 | インヴィトロゲン・コーポレーション | 大腸菌トポイソメラーゼiii及びその相同体を使用して全長核酸配列を得る方法 |
US7485715B2 (en) | 1999-06-18 | 2009-02-03 | Ceres, Inc. | Sequence-determined DNA encoding AP2 domain polypeptides |
US7479555B2 (en) | 1999-07-21 | 2009-01-20 | Ceres, Inc. | Polynucleotides having a nucleotide sequence that encodes a polypeptide having MOV34 family activity |
CA2311201A1 (en) * | 1999-08-05 | 2001-02-05 | Genset S.A. | Ests and encoded human proteins |
US20020058617A1 (en) | 2000-01-14 | 2002-05-16 | Joachim Fruebis | OBG3 globular head and uses thereof for decreasing body mass |
US6566332B2 (en) | 2000-01-14 | 2003-05-20 | Genset S.A. | OBG3 globular head and uses thereof for decreasing body mass |
US6989367B2 (en) | 2000-01-14 | 2006-01-24 | Genset S.A. | OBG3 globular head and uses thereof |
US7338787B2 (en) | 2000-01-14 | 2008-03-04 | Serono Genetics Institute S.A. | Nucleic acids encoding OBG3 globular head and uses thereof |
EP1261724A4 (de) | 2000-02-25 | 2004-08-18 | Invitrogen Corp | Topoisomerase linker-vermittelte amplifizierungsverfahren |
US6387624B1 (en) * | 2000-04-14 | 2002-05-14 | Incyte Pharmaceuticals, Inc. | Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
WO2002016594A2 (en) | 2000-08-21 | 2002-02-28 | Invitrogen Corporation | Methods and reagents for molecular cloning |
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JP4517114B2 (ja) * | 2003-03-28 | 2010-08-04 | 国立障害者リハビリテーションセンター総長 | cDNA合成方法 |
EP1697534B1 (de) | 2003-12-01 | 2010-06-02 | Life Technologies Corporation | Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon |
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US8206924B2 (en) * | 2006-09-11 | 2012-06-26 | Osaka University | Trace mRNA amplification method and use thereof |
CN101932712B (zh) | 2007-11-20 | 2014-05-14 | 先锋国际良种公司 | 用于改善植物应激耐性的玉米乙烯信号转导基因及其调节 |
US20100035249A1 (en) * | 2008-08-05 | 2010-02-11 | Kabushiki Kaisha Dnaform | Rna sequencing and analysis using solid support |
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WO2011017492A2 (en) | 2009-08-05 | 2011-02-10 | Pioneer Hi-Bred International, Inc. | Novel eto1 genes and use of same for reduced ethylene and improved stress tolerance in plants |
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CN103906835A (zh) | 2011-10-25 | 2014-07-02 | 先锋国际良种公司 | 改变植物细胞壁组成以改善生物燃料生产和青贮饲料可消化性的方法 |
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US10323076B2 (en) | 2013-10-03 | 2019-06-18 | Modernatx, Inc. | Polynucleotides encoding low density lipoprotein receptor |
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ES2914225T3 (es) | 2015-10-16 | 2022-06-08 | Modernatx Inc | Análogos de cap de ARNm con enlace de fosfato modificado |
US11866754B2 (en) | 2015-10-16 | 2024-01-09 | Modernatx, Inc. | Trinucleotide mRNA cap analogs |
EP3409773B1 (de) | 2016-01-27 | 2020-07-29 | Kabushiki Kaisha DNAFORM | Verfahren zur decodierung einer basensequenz von nukleinsäuren entsprechend einer terminalen rns-region und verfahren zur analyse eines dns-elements |
CN109937253B (zh) | 2016-09-14 | 2023-06-30 | 摩登纳特斯有限公司 | 高纯度rna组合物及其制备方法 |
WO2019036683A1 (en) | 2017-08-18 | 2019-02-21 | Modernatx, Inc. | ANALYTICAL METHODS BY HPLC |
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Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4661450A (en) * | 1983-05-03 | 1987-04-28 | Molecular Genetics Research And Development Limited Partnership | Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides |
US4710566A (en) * | 1984-12-27 | 1987-12-01 | Yale University | Purification of the messenger RNA cap-binding protein |
US5273879A (en) * | 1987-07-23 | 1993-12-28 | Syntex (U.S.A.) Inc. | Amplification method for polynucleotide assays |
US5219989A (en) * | 1988-12-13 | 1993-06-15 | Mcgill University | Bifunctional protein for the isolation of capped mRNA |
CA2005252C (en) * | 1988-12-13 | 2001-07-17 | Nahum Sonenberg | Isolation of full-length cdna clones and capped mrna |
US5324829A (en) * | 1988-12-16 | 1994-06-28 | Ortho Diagnostic Systems, Inc. | High specific activity nucleic acid probes having target recognition and signal generating moieties |
JP3315439B2 (ja) * | 1992-07-21 | 2002-08-19 | 住友電気工業株式会社 | 細胞特異的cDNAの分離法及びライブラリー作製法 |
JP3337748B2 (ja) * | 1992-09-25 | 2002-10-21 | 財団法人神奈川科学技術アカデミー | 完全長cDNAの合成方法、その中間体の製造方法及び完全長cDNAを含む組換えベクターの製造方法 |
-
1995
- 1995-08-03 FR FR9509467A patent/FR2733762B1/fr not_active Expired - Fee Related
-
1996
- 1996-04-29 WO PCT/FR1996/000651 patent/WO1996034981A2/fr active IP Right Grant
- 1996-04-29 DE DE69624695T patent/DE69624695T2/de not_active Expired - Fee Related
- 1996-04-29 AT AT96914259T patent/ATE227346T1/de not_active IP Right Cessation
- 1996-04-29 DK DK96914259T patent/DK0824598T3/da active
- 1996-04-29 JP JP8533070A patent/JPH11510364A/ja not_active Ceased
- 1996-04-29 AU AU59829/96A patent/AU719217B2/en not_active Ceased
- 1996-04-29 CA CA002220045A patent/CA2220045C/fr not_active Expired - Fee Related
- 1996-04-29 PT PT96914259T patent/PT824598E/pt unknown
- 1996-04-29 US US08/930,102 patent/US6022715A/en not_active Expired - Fee Related
- 1996-04-29 ES ES96914259T patent/ES2186778T3/es not_active Expired - Lifetime
- 1996-04-29 EP EP96914259A patent/EP0824598B1/de not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP0824598B1 (de) | 2002-11-06 |
US6022715A (en) | 2000-02-08 |
FR2733762A1 (fr) | 1996-11-08 |
WO1996034981A2 (fr) | 1996-11-07 |
PT824598E (pt) | 2003-03-31 |
FR2733762B1 (fr) | 1997-08-01 |
ES2186778T3 (es) | 2003-05-16 |
EP0824598A2 (de) | 1998-02-25 |
ATE227346T1 (de) | 2002-11-15 |
CA2220045A1 (fr) | 1996-11-07 |
DK0824598T3 (da) | 2003-03-10 |
WO1996034981A3 (fr) | 1997-01-03 |
AU719217B2 (en) | 2000-05-04 |
AU5982996A (en) | 1996-11-21 |
CA2220045C (fr) | 2001-10-30 |
JPH11510364A (ja) | 1999-09-14 |
DE69624695T2 (de) | 2003-07-31 |
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