FR2733765B1 - Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm - Google Patents

Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm

Info

Publication number
FR2733765B1
FR2733765B1 FR9505221A FR9505221A FR2733765B1 FR 2733765 B1 FR2733765 B1 FR 2733765B1 FR 9505221 A FR9505221 A FR 9505221A FR 9505221 A FR9505221 A FR 9505221A FR 2733765 B1 FR2733765 B1 FR 2733765B1
Authority
FR
France
Prior art keywords
rnam
fragment
hair
specific coupling
coupling
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
FR9505221A
Other languages
English (en)
Other versions
FR2733765A1 (fr
Inventor
Merenkova Irena Nicolaevna
Milne Edwards Jean Bapti Dumas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Biodevelopment SAS
Original Assignee
Genset SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genset SA filed Critical Genset SA
Priority to FR9505221A priority Critical patent/FR2733765B1/fr
Priority to FR9509467A priority patent/FR2733762B1/fr
Priority to CA002220045A priority patent/CA2220045C/fr
Priority to JP8533070A priority patent/JPH11510364A/ja
Priority to US08/930,102 priority patent/US6022715A/en
Priority to EP96914259A priority patent/EP0824598B1/fr
Priority to PCT/FR1996/000651 priority patent/WO1996034981A2/fr
Priority to DE69624695T priority patent/DE69624695T2/de
Priority to AT96914259T priority patent/ATE227346T1/de
Priority to PT96914259T priority patent/PT824598E/pt
Priority to AU59829/96A priority patent/AU719217B2/en
Priority to DK96914259T priority patent/DK0824598T3/da
Priority to ES96914259T priority patent/ES2186778T3/es
Publication of FR2733765A1 publication Critical patent/FR2733765A1/fr
Application granted granted Critical
Publication of FR2733765B1 publication Critical patent/FR2733765B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
FR9505221A 1995-05-02 1995-05-02 Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm Expired - Lifetime FR2733765B1 (fr)

Priority Applications (13)

Application Number Priority Date Filing Date Title
FR9505221A FR2733765B1 (fr) 1995-05-02 1995-05-02 Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm
FR9509467A FR2733762B1 (fr) 1995-05-02 1995-08-03 Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet
PT96914259T PT824598E (pt) 1995-05-02 1996-04-29 Processo para o acoplamento especifico de um grupo de bloqueio da extremidade 5' de um fragmento de arnm e preparacao do arnm e do adnc completo
US08/930,102 US6022715A (en) 1995-05-02 1996-04-29 Method for the specific coupling of the cap of the 5' end of an mRNA fragment and preparation of mRNA and complete cDNA
EP96914259A EP0824598B1 (fr) 1995-05-02 1996-04-29 METHODE DE COUPLAGE SPECIFIQUE DE LA COIFFE DE L'EXTREMITE 5' D'UN FRAGMENT D'ARNm ET PREPARATION D'ARNm ET D'ADNc COMPLET
PCT/FR1996/000651 WO1996034981A2 (fr) 1995-05-02 1996-04-29 METHODE DE COUPLAGE SPECIFIQUE DE LA COIFFE DE L'EXTREMITE 5' D'UN FRAGMENT D'ARNm ET PREPARATION D'ARNm ET D'ADNc COMPLET
CA002220045A CA2220045C (fr) 1995-05-02 1996-04-29 Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet
AT96914259T ATE227346T1 (de) 1995-05-02 1996-04-29 Verfahren zur spezifischen kopplung einer schutzgruppe an das 5'-ende eines mrna-fragmentes und die herstellung von mrna und vollständiger cdna
JP8533070A JPH11510364A (ja) 1995-05-02 1996-04-29 ｍＲＮＡ断片の５′末端のキャップの特異的カップリングの方法、およびｍＲＮＡおよび完全なｃＤＮＡの調製
AU59829/96A AU719217B2 (en) 1995-05-02 1996-04-29 Method for the specific coupling of the cap of the 5' end of an mRNA fragment and preparation of mRNA and of complete cDNA
DK96914259T DK0824598T3 (da) 1995-05-02 1996-04-29 Fremgangsmåde til specifik kobling af kappen på ende-5' af et mRNA fragment og fremstilling af komplet mRNA og cDNA
ES96914259T ES2186778T3 (es) 1995-05-02 1996-04-29 Metodo de acoplamiento especifico de la caperuza del extremo 5' de un fragmento de arnm y preparacion del arnm y del adnc completo.
DE69624695T DE69624695T2 (de) 1995-05-02 1996-04-29 VERFAHREN ZUR SPEZIFISCHEN KOPPLUNG EINER SCHUTZGRUPPE AN DAS 5'-ENDE EINES mRNA-FRAGMENTES UND DIE HERSTELLUNG VON mRNA UND VOLLSTÄNDIGER cDNA

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9505221A FR2733765B1 (fr) 1995-05-02 1995-05-02 Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm

Publications (2)

Publication Number Publication Date
FR2733765A1 FR2733765A1 (fr) 1996-11-08
FR2733765B1 true FR2733765B1 (fr) 1997-07-25

Family

ID=9478607

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9505221A Expired - Lifetime FR2733765B1 (fr) 1995-05-02 1995-05-02 Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm

Country Status (1)

Country Link
FR (1) FR2733765B1 (fr)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4710566A (en) * 1984-12-27 1987-12-01 Yale University Purification of the messenger RNA cap-binding protein
CA2005252C (fr) * 1988-12-13 2001-07-17 Nahum Sonenberg Isolement de clones d'adn-c pleine longueur et de mrna chapeautes
US5324829A (en) * 1988-12-16 1994-06-28 Ortho Diagnostic Systems, Inc. High specific activity nucleic acid probes having target recognition and signal generating moieties
JP3315439B2 (ja) * 1992-07-21 2002-08-19 住友電気工業株式会社 細胞特異的ｃＤＮＡの分離法及びライブラリー作製法
JP3337748B2 (ja) * 1992-09-25 2002-10-21 財団法人神奈川科学技術アカデミー 完全長ｃＤＮＡの合成方法、その中間体の製造方法及び完全長ｃＤＮＡを含む組換えベクターの製造方法

Also Published As

Publication number Publication date
FR2733765A1 (fr) 1996-11-08

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