DE69624695T2 - VERFAHREN ZUR SPEZIFISCHEN KOPPLUNG EINER SCHUTZGRUPPE AN DAS 5'-ENDE EINES mRNA-FRAGMENTES UND DIE HERSTELLUNG VON mRNA UND VOLLSTÄNDIGER cDNA - Google Patents
VERFAHREN ZUR SPEZIFISCHEN KOPPLUNG EINER SCHUTZGRUPPE AN DAS 5'-ENDE EINES mRNA-FRAGMENTES UND DIE HERSTELLUNG VON mRNA UND VOLLSTÄNDIGER cDNAInfo
- Publication number
- DE69624695T2 DE69624695T2 DE69624695T DE69624695T DE69624695T2 DE 69624695 T2 DE69624695 T2 DE 69624695T2 DE 69624695 T DE69624695 T DE 69624695T DE 69624695 T DE69624695 T DE 69624695T DE 69624695 T2 DE69624695 T2 DE 69624695T2
- Authority
- DE
- Germany
- Prior art keywords
- mrna
- pct
- specific coupling
- production
- fragment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 108020004999 messenger RNA Proteins 0.000 title abstract 7
- 238000000034 method Methods 0.000 title abstract 5
- 239000002299 complementary DNA Substances 0.000 title abstract 4
- 230000008878 coupling Effects 0.000 title abstract 2
- 238000010168 coupling process Methods 0.000 title abstract 2
- 238000005859 coupling reaction Methods 0.000 title abstract 2
- 239000012634 fragment Substances 0.000 title abstract 2
- 230000001681 protective effect Effects 0.000 title 1
- 125000003277 amino group Chemical group 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Mechanical Coupling Of Light Guides (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Cosmetics (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9505221A FR2733765B1 (fr) | 1995-05-02 | 1995-05-02 | Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm |
FR9509467A FR2733762B1 (fr) | 1995-05-02 | 1995-08-03 | Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet |
PCT/FR1996/000651 WO1996034981A2 (fr) | 1995-05-02 | 1996-04-29 | METHODE DE COUPLAGE SPECIFIQUE DE LA COIFFE DE L'EXTREMITE 5' D'UN FRAGMENT D'ARNm ET PREPARATION D'ARNm ET D'ADNc COMPLET |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69624695D1 DE69624695D1 (de) | 2002-12-12 |
DE69624695T2 true DE69624695T2 (de) | 2003-07-31 |
Family
ID=26231928
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69624695T Expired - Fee Related DE69624695T2 (de) | 1995-05-02 | 1996-04-29 | VERFAHREN ZUR SPEZIFISCHEN KOPPLUNG EINER SCHUTZGRUPPE AN DAS 5'-ENDE EINES mRNA-FRAGMENTES UND DIE HERSTELLUNG VON mRNA UND VOLLSTÄNDIGER cDNA |
Country Status (12)
Country | Link |
---|---|
US (1) | US6022715A (de) |
EP (1) | EP0824598B1 (de) |
JP (1) | JPH11510364A (de) |
AT (1) | ATE227346T1 (de) |
AU (1) | AU719217B2 (de) |
CA (1) | CA2220045C (de) |
DE (1) | DE69624695T2 (de) |
DK (1) | DK0824598T3 (de) |
ES (1) | ES2186778T3 (de) |
FR (1) | FR2733762B1 (de) |
PT (1) | PT824598E (de) |
WO (1) | WO1996034981A2 (de) |
Families Citing this family (90)
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US5766891A (en) | 1994-12-19 | 1998-06-16 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
JP3441899B2 (ja) * | 1996-11-01 | 2003-09-02 | 理化学研究所 | 完全長cDNAライブラリーの作成方法 |
AU746657B2 (en) | 1997-06-12 | 2002-05-02 | Invitrogen Corporation | Covalent joining of DNA strands to RNA strands catalyzed by vaccinia topoisomerase |
EP1367124A1 (de) * | 1997-08-01 | 2003-12-03 | Genset | 5' Ests für sekretierte Proteine, die im Muskel und anderen mesodermalen Geweben exprimiert werden |
WO1999006553A2 (en) * | 1997-08-01 | 1999-02-11 | Genset | 5' ESTs FOR SECRETED PROTEINS EXPRESSED IN VARIOUS TISSUES |
US7393663B2 (en) | 1997-08-01 | 2008-07-01 | Serono Genetics Institute S.A. | Expressed sequence tags and encoded human proteins |
JP2001512013A (ja) * | 1997-08-01 | 2001-08-21 | ジェンセット | 前立腺に発現される分泌タンパク質の5’est |
JP2001512012A (ja) * | 1997-08-01 | 2001-08-21 | ジェンセット | 精巣およびその他の組織で発現する分泌タンパク質の5’est |
WO1999006548A2 (en) * | 1997-08-01 | 1999-02-11 | Genset | 5'ESTs FOR NON TISSUE SPECIFIC SECRETED PROTEINS |
WO1999006551A2 (en) * | 1997-08-01 | 1999-02-11 | Genset | 5' ESTs FOR SECRETED PROTEINS IDENTIFIED FROM BRAIN TISSUES |
AU8555298A (en) * | 1997-08-01 | 1999-02-22 | Genset | 5' ests for secreted proteins expressed in endoderm |
US6222029B1 (en) * | 1997-08-01 | 2001-04-24 | Genset | 5′ ESTs for secreted proteins expressed in brain |
FR2767135B1 (fr) | 1997-08-06 | 2002-07-12 | Genset Sa | Recepteur complexe lsr, activite, clonage, et application au diagnostic, a la prevention et/ou au traitement de d'obesite et des risques ou complications associes |
WO1999020750A1 (fr) * | 1997-10-22 | 1999-04-29 | Helix Research Institute | Procede destine au criblage des clones d'adn-c pleine longueur |
US6573068B1 (en) | 1997-11-13 | 2003-06-03 | Genset, S. A. | Claudin-50 protein |
US20060286639A1 (en) * | 1997-11-13 | 2006-12-21 | Serono Genetics Institute S.A. | Complementary DNAs encoding proteins with signal peptides |
US6548633B1 (en) | 1998-12-22 | 2003-04-15 | Genset, S.A. | Complementary DNA's encoding proteins with signal peptides |
EP1903111A3 (de) * | 1997-11-13 | 2008-07-02 | Serono Genetics Institute S.A. | Erweiterte cDNA für sekretierte Proteine |
JP2001523453A (ja) * | 1997-11-13 | 2001-11-27 | ジェンセット | 分泌タンパク質の伸長cDNA |
WO1999040189A2 (en) * | 1998-02-09 | 1999-08-12 | Genset | Cdnas encoding secreted proteins |
EP1037977B1 (de) * | 1997-12-17 | 2009-08-26 | Serono Genetics Institute S.A. | Verlängerte cdns, die für sekretierte proteine kodieren |
US6783961B1 (en) | 1999-02-26 | 2004-08-31 | Genset S.A. | Expressed sequence tags and encoded human proteins |
CA2319089A1 (en) | 1998-04-09 | 1999-10-21 | Genset S.A. | 5' ests and encoded human proteins |
US7223727B2 (en) | 1998-04-09 | 2007-05-29 | Serono Genetics Institute S.A. | GSSP4 polynucleotides and polypeptides and uses thereof |
AU772243B2 (en) | 1998-11-04 | 2004-04-22 | Genset | Genomic and complete cDNA sequences of human adipocyte-specific APM1 and biallelic markers thereof |
US6455280B1 (en) | 1998-12-22 | 2002-09-24 | Genset S.A. | Methods and compositions for inhibiting neoplastic cell growth |
ATE427362T1 (de) | 1999-02-10 | 2009-04-15 | Serono Genetics Inst Sa | Polymorphe marker des lsr-gens |
EP1586645A3 (de) | 1999-02-25 | 2006-02-22 | Ceres Incorporated | DNA-Fragmente mit bestimmter Sequenz und die dadurch kodierte Polypeptide |
JP2002539784A (ja) * | 1999-03-19 | 2002-11-26 | インヴィトロゲン・コーポレーション | 大腸菌トポイソメラーゼiii及びその相同体を使用して全長核酸配列を得る方法 |
JP2002539799A (ja) * | 1999-03-19 | 2002-11-26 | ジェネティックス・インスチチュート・インコーポレーテッド | プライマー結合ベクター伸長(PAVE):5’−指向cDNAクローニング法 |
US7485715B2 (en) | 1999-06-18 | 2009-02-03 | Ceres, Inc. | Sequence-determined DNA encoding AP2 domain polypeptides |
US7479555B2 (en) | 1999-07-21 | 2009-01-20 | Ceres, Inc. | Polynucleotides having a nucleotide sequence that encodes a polypeptide having MOV34 family activity |
CA2311201A1 (en) | 1999-08-05 | 2001-02-05 | Genset S.A. | Ests and encoded human proteins |
US6989367B2 (en) | 2000-01-14 | 2006-01-24 | Genset S.A. | OBG3 globular head and uses thereof |
US20020058617A1 (en) | 2000-01-14 | 2002-05-16 | Joachim Fruebis | OBG3 globular head and uses thereof for decreasing body mass |
US6566332B2 (en) | 2000-01-14 | 2003-05-20 | Genset S.A. | OBG3 globular head and uses thereof for decreasing body mass |
US7338787B2 (en) | 2000-01-14 | 2008-03-04 | Serono Genetics Institute S.A. | Nucleic acids encoding OBG3 globular head and uses thereof |
US7078501B2 (en) | 2000-02-25 | 2006-07-18 | Invitrogen Corporation | Topoisomerase linker-mediated amplification methods |
US6387624B1 (en) * | 2000-04-14 | 2002-05-14 | Incyte Pharmaceuticals, Inc. | Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
AU8835601A (en) | 2000-08-21 | 2002-03-04 | Invitrogen Corp | Methods and reagents for molecular cloning |
US6875907B2 (en) | 2000-09-13 | 2005-04-05 | Pioneer Hi-Bred International, Inc. | Antimicrobial peptides and methods of use |
NZ526194A (en) | 2000-12-08 | 2004-10-29 | Invitrogen Corp | Methods of covalently linking, in one or both strands, two or more double stranded nucleotide sequences using one or more topoisomerases |
US20060008817A1 (en) | 2000-12-08 | 2006-01-12 | Invitrogen Corporation | Methods and compositions for generating recombinant nucleic acid molecules |
MXPA04002817A (es) | 2001-09-27 | 2004-07-05 | Pioneer Hi Bred Int | Polinucleotidos de fitato y metodos de uso. |
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WO2003106672A2 (en) * | 2002-06-12 | 2003-12-24 | Riken | METHOD FOR UTILIZING THE 5'END OF mRNA FOR CLONING AND ANALYSIS |
US8067205B2 (en) * | 2003-03-28 | 2011-11-29 | Japan As Represented By Director General Of National Rehabilitation Center For Persons With Disabilities | Method of synthesizing cDNA |
JP2007512838A (ja) | 2003-12-01 | 2007-05-24 | インヴィトロジェン コーポレーション | 組換え部位を含む核酸分子およびその使用方法 |
GB0417887D0 (en) * | 2004-08-11 | 2004-09-15 | Ares Trading Sa | Protein |
US8206924B2 (en) * | 2006-09-11 | 2012-06-26 | Osaka University | Trace mRNA amplification method and use thereof |
MX2010005565A (es) | 2007-11-20 | 2010-06-11 | Pioneer Hi Bred Int | Genes de señalizacion de etileno en maiz y su modulacion para mejor tolerancia al estres en plantas. |
US20100035249A1 (en) * | 2008-08-05 | 2010-02-11 | Kabushiki Kaisha Dnaform | Rna sequencing and analysis using solid support |
US8716553B2 (en) | 2009-03-02 | 2014-05-06 | Pioneer Hi Bred International Inc | NAC transcriptional activators involved in abiotic stress tolerance |
WO2011017492A2 (en) | 2009-08-05 | 2011-02-10 | Pioneer Hi-Bred International, Inc. | Novel eto1 genes and use of same for reduced ethylene and improved stress tolerance in plants |
WO2012019168A2 (en) | 2010-08-06 | 2012-02-09 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
DE19177059T1 (de) | 2010-10-01 | 2021-10-07 | Modernatx, Inc. | N1-methyl-pseudouracile enthältendes ribonucleinsäuren sowie ihre verwendungen |
DE12722942T1 (de) | 2011-03-31 | 2021-09-30 | Modernatx, Inc. | Freisetzung und formulierung von manipulierten nukleinsäuren |
US9464124B2 (en) | 2011-09-12 | 2016-10-11 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
JP6113737B2 (ja) | 2011-10-03 | 2017-04-12 | モデルナティエックス インコーポレイテッドModernaTX,Inc. | 修飾型のヌクレオシド、ヌクレオチドおよび核酸、ならびにそれらの使用方法 |
AR088515A1 (es) | 2011-10-25 | 2014-06-18 | Pioneer Hi Bred Int | Metodos para alterar la composicion de la pared celular vegetal para obtener produccion de biocombustible y digestibilidad de silaje mejoradas |
MX2014007233A (es) | 2011-12-16 | 2015-02-04 | Moderna Therapeutics Inc | Composiciones de nucleosidos, nucleotidos y acidos nucleicos modificados. |
US9572897B2 (en) | 2012-04-02 | 2017-02-21 | Modernatx, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
US9283287B2 (en) | 2012-04-02 | 2016-03-15 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of nuclear proteins |
EP2834259A4 (de) | 2012-04-02 | 2016-08-24 | Moderna Therapeutics Inc | Modifizierte polynukleotide |
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US11866754B2 (en) | 2015-10-16 | 2024-01-09 | Modernatx, Inc. | Trinucleotide mRNA cap analogs |
WO2017066781A1 (en) | 2015-10-16 | 2017-04-20 | Modernatx, Inc. | Mrna cap analogs with modified phosphate linkage |
WO2017130750A1 (ja) | 2016-01-27 | 2017-08-03 | 株式会社ダナフォーム | Rnaの末端領域に対応する核酸の塩基配列を解読する方法およびdnaエレメントの分析方法 |
MX2019002904A (es) | 2016-09-14 | 2019-09-26 | Modernatx Inc | Composiciones de arn de alta pureza y métodos para su preparación. |
EP3668977A4 (de) | 2017-08-18 | 2021-04-21 | Modernatx, Inc. | Analytische hplc-verfahren |
WO2019036685A1 (en) | 2017-08-18 | 2019-02-21 | Modernatx, Inc. | METHODS FOR HPLC ANALYSIS |
WO2023127774A1 (ja) * | 2021-12-28 | 2023-07-06 | 株式会社ダナフォーム | 遺伝子包含体の検出方法及び検出用キット |
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Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4661450A (en) * | 1983-05-03 | 1987-04-28 | Molecular Genetics Research And Development Limited Partnership | Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides |
US4710566A (en) * | 1984-12-27 | 1987-12-01 | Yale University | Purification of the messenger RNA cap-binding protein |
US5273879A (en) * | 1987-07-23 | 1993-12-28 | Syntex (U.S.A.) Inc. | Amplification method for polynucleotide assays |
CA2005252C (en) * | 1988-12-13 | 2001-07-17 | Nahum Sonenberg | Isolation of full-length cdna clones and capped mrna |
US5219989A (en) * | 1988-12-13 | 1993-06-15 | Mcgill University | Bifunctional protein for the isolation of capped mRNA |
US5324829A (en) * | 1988-12-16 | 1994-06-28 | Ortho Diagnostic Systems, Inc. | High specific activity nucleic acid probes having target recognition and signal generating moieties |
JP3315439B2 (ja) * | 1992-07-21 | 2002-08-19 | 住友電気工業株式会社 | 細胞特異的cDNAの分離法及びライブラリー作製法 |
JP3337748B2 (ja) * | 1992-09-25 | 2002-10-21 | 財団法人神奈川科学技術アカデミー | 完全長cDNAの合成方法、その中間体の製造方法及び完全長cDNAを含む組換えベクターの製造方法 |
-
1995
- 1995-08-03 FR FR9509467A patent/FR2733762B1/fr not_active Expired - Fee Related
-
1996
- 1996-04-29 EP EP96914259A patent/EP0824598B1/de not_active Expired - Lifetime
- 1996-04-29 DE DE69624695T patent/DE69624695T2/de not_active Expired - Fee Related
- 1996-04-29 PT PT96914259T patent/PT824598E/pt unknown
- 1996-04-29 AU AU59829/96A patent/AU719217B2/en not_active Ceased
- 1996-04-29 AT AT96914259T patent/ATE227346T1/de not_active IP Right Cessation
- 1996-04-29 US US08/930,102 patent/US6022715A/en not_active Expired - Fee Related
- 1996-04-29 CA CA002220045A patent/CA2220045C/fr not_active Expired - Fee Related
- 1996-04-29 ES ES96914259T patent/ES2186778T3/es not_active Expired - Lifetime
- 1996-04-29 WO PCT/FR1996/000651 patent/WO1996034981A2/fr active IP Right Grant
- 1996-04-29 DK DK96914259T patent/DK0824598T3/da active
- 1996-04-29 JP JP8533070A patent/JPH11510364A/ja not_active Ceased
Also Published As
Publication number | Publication date |
---|---|
DE69624695D1 (de) | 2002-12-12 |
ATE227346T1 (de) | 2002-11-15 |
EP0824598A2 (de) | 1998-02-25 |
AU719217B2 (en) | 2000-05-04 |
AU5982996A (en) | 1996-11-21 |
FR2733762A1 (fr) | 1996-11-08 |
CA2220045A1 (fr) | 1996-11-07 |
EP0824598B1 (de) | 2002-11-06 |
PT824598E (pt) | 2003-03-31 |
CA2220045C (fr) | 2001-10-30 |
FR2733762B1 (fr) | 1997-08-01 |
WO1996034981A3 (fr) | 1997-01-03 |
US6022715A (en) | 2000-02-08 |
WO1996034981A2 (fr) | 1996-11-07 |
JPH11510364A (ja) | 1999-09-14 |
DK0824598T3 (da) | 2003-03-10 |
ES2186778T3 (es) | 2003-05-16 |
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