DE69624695T2 - VERFAHREN ZUR SPEZIFISCHEN KOPPLUNG EINER SCHUTZGRUPPE AN DAS 5'-ENDE EINES mRNA-FRAGMENTES UND DIE HERSTELLUNG VON mRNA UND VOLLSTÄNDIGER cDNA - Google Patents

VERFAHREN ZUR SPEZIFISCHEN KOPPLUNG EINER SCHUTZGRUPPE AN DAS 5'-ENDE EINES mRNA-FRAGMENTES UND DIE HERSTELLUNG VON mRNA UND VOLLSTÄNDIGER cDNA

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Publication number
DE69624695T2
DE69624695T2 DE69624695T DE69624695T DE69624695T2 DE 69624695 T2 DE69624695 T2 DE 69624695T2 DE 69624695 T DE69624695 T DE 69624695T DE 69624695 T DE69624695 T DE 69624695T DE 69624695 T2 DE69624695 T2 DE 69624695T2
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DE
Germany
Prior art keywords
mrna
pct
specific coupling
production
fragment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69624695T
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English (en)
Other versions
DE69624695D1 (de
Inventor
Irena Nicolaevna Merenkova
Milne Edwards Gabriel Dumas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Biodevelopment SAS
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Serono Genetics Institute SA
Genset SA
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Filing date
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Priority claimed from FR9505221A external-priority patent/FR2733765B1/fr
Application filed by Serono Genetics Institute SA, Genset SA filed Critical Serono Genetics Institute SA
Publication of DE69624695D1 publication Critical patent/DE69624695D1/de
Application granted granted Critical
Publication of DE69624695T2 publication Critical patent/DE69624695T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Mechanical Coupling Of Light Guides (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Cosmetics (AREA)
  • Saccharide Compounds (AREA)
DE69624695T 1995-05-02 1996-04-29 VERFAHREN ZUR SPEZIFISCHEN KOPPLUNG EINER SCHUTZGRUPPE AN DAS 5'-ENDE EINES mRNA-FRAGMENTES UND DIE HERSTELLUNG VON mRNA UND VOLLSTÄNDIGER cDNA Expired - Fee Related DE69624695T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9505221A FR2733765B1 (fr) 1995-05-02 1995-05-02 Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm
FR9509467A FR2733762B1 (fr) 1995-05-02 1995-08-03 Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet
PCT/FR1996/000651 WO1996034981A2 (fr) 1995-05-02 1996-04-29 METHODE DE COUPLAGE SPECIFIQUE DE LA COIFFE DE L'EXTREMITE 5' D'UN FRAGMENT D'ARNm ET PREPARATION D'ARNm ET D'ADNc COMPLET

Publications (2)

Publication Number Publication Date
DE69624695D1 DE69624695D1 (de) 2002-12-12
DE69624695T2 true DE69624695T2 (de) 2003-07-31

Family

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DE69624695T Expired - Fee Related DE69624695T2 (de) 1995-05-02 1996-04-29 VERFAHREN ZUR SPEZIFISCHEN KOPPLUNG EINER SCHUTZGRUPPE AN DAS 5'-ENDE EINES mRNA-FRAGMENTES UND DIE HERSTELLUNG VON mRNA UND VOLLSTÄNDIGER cDNA

Country Status (12)

Country Link
US (1) US6022715A (de)
EP (1) EP0824598B1 (de)
JP (1) JPH11510364A (de)
AT (1) ATE227346T1 (de)
AU (1) AU719217B2 (de)
CA (1) CA2220045C (de)
DE (1) DE69624695T2 (de)
DK (1) DK0824598T3 (de)
ES (1) ES2186778T3 (de)
FR (1) FR2733762B1 (de)
PT (1) PT824598E (de)
WO (1) WO1996034981A2 (de)

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Also Published As

Publication number Publication date
DE69624695D1 (de) 2002-12-12
ATE227346T1 (de) 2002-11-15
EP0824598A2 (de) 1998-02-25
AU719217B2 (en) 2000-05-04
AU5982996A (en) 1996-11-21
FR2733762A1 (fr) 1996-11-08
CA2220045A1 (fr) 1996-11-07
EP0824598B1 (de) 2002-11-06
PT824598E (pt) 2003-03-31
CA2220045C (fr) 2001-10-30
FR2733762B1 (fr) 1997-08-01
WO1996034981A3 (fr) 1997-01-03
US6022715A (en) 2000-02-08
WO1996034981A2 (fr) 1996-11-07
JPH11510364A (ja) 1999-09-14
DK0824598T3 (da) 2003-03-10
ES2186778T3 (es) 2003-05-16

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