PT824598E - Processo para o acoplamento especifico de um grupo de bloqueio da extremidade 5' de um fragmento de arnm e preparacao do arnm e do adnc completo - Google Patents

Processo para o acoplamento especifico de um grupo de bloqueio da extremidade 5' de um fragmento de arnm e preparacao do arnm e do adnc completo

Info

Publication number
PT824598E
PT824598E PT96914259T PT96914259T PT824598E PT 824598 E PT824598 E PT 824598E PT 96914259 T PT96914259 T PT 96914259T PT 96914259 T PT96914259 T PT 96914259T PT 824598 E PT824598 E PT 824598E
Authority
PT
Portugal
Prior art keywords
mrna
pct
preparation
fragment
specific coupling
Prior art date
Application number
PT96914259T
Other languages
English (en)
Inventor
Irena Merenkova
Jean-Baptiste Gabriel Edwards
Original Assignee
Genset Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR9505221A external-priority patent/FR2733765B1/fr
Application filed by Genset Sa filed Critical Genset Sa
Publication of PT824598E publication Critical patent/PT824598E/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Cosmetics (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Mechanical Coupling Of Light Guides (AREA)
PT96914259T 1995-05-02 1996-04-29 Processo para o acoplamento especifico de um grupo de bloqueio da extremidade 5' de um fragmento de arnm e preparacao do arnm e do adnc completo PT824598E (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9505221A FR2733765B1 (fr) 1995-05-02 1995-05-02 Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm
FR9509467A FR2733762B1 (fr) 1995-05-02 1995-08-03 Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet

Publications (1)

Publication Number Publication Date
PT824598E true PT824598E (pt) 2003-03-31

Family

ID=26231928

Family Applications (1)

Application Number Title Priority Date Filing Date
PT96914259T PT824598E (pt) 1995-05-02 1996-04-29 Processo para o acoplamento especifico de um grupo de bloqueio da extremidade 5' de um fragmento de arnm e preparacao do arnm e do adnc completo

Country Status (12)

Country Link
US (1) US6022715A (pt)
EP (1) EP0824598B1 (pt)
JP (1) JPH11510364A (pt)
AT (1) ATE227346T1 (pt)
AU (1) AU719217B2 (pt)
CA (1) CA2220045C (pt)
DE (1) DE69624695T2 (pt)
DK (1) DK0824598T3 (pt)
ES (1) ES2186778T3 (pt)
FR (1) FR2733762B1 (pt)
PT (1) PT824598E (pt)
WO (1) WO1996034981A2 (pt)

Families Citing this family (90)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
JP3441899B2 (ja) * 1996-11-01 2003-09-02 理化学研究所 完全長ｃＤＮＡライブラリーの作成方法
CA2263716C (en) 1997-06-12 2007-09-25 Sloan-Kettering Institute For Cancer Research Covalent joining of dna strands to rna strands catalyzed by vaccinia topoisomerase
CA2296844A1 (en) * 1997-08-01 1999-02-11 Genset 5' ests for secreted proteins expressed in various tissues
US7393663B2 (en) 1997-08-01 2008-07-01 Serono Genetics Institute S.A. Expressed sequence tags and encoded human proteins
AU8555298A (en) * 1997-08-01 1999-02-22 Genset 5' ests for secreted proteins expressed in endoderm
EP1000147A2 (en) * 1997-08-01 2000-05-17 Serono Genetics Institute S.A. 5' ESTs FOR SECRETED PROTEINS EXPRESSED IN TESTIS AND OTHER TISSUES
WO1999006550A2 (en) * 1997-08-01 1999-02-11 Genset 5' ESTs FOR SECRETED PROTEINS EXPRESSED IN PROSTATE
PT1000146E (pt) * 1997-08-01 2006-10-31 Serono Genetics Inst Sa Ests 5' para proteinas secretadas sem especificidade tecidular
US6222029B1 (en) * 1997-08-01 2001-04-24 Genset 5′ ESTs for secreted proteins expressed in brain
AU8555498A (en) * 1997-08-01 1999-02-22 Genset 5' ests for secreted proteins identified from brain tissues
AU8555798A (en) * 1997-08-01 1999-02-22 Genset 5' ests for secreted proteins expressed in muscle and other mesodermal tissues
FR2767135B1 (fr) 1997-08-06 2002-07-12 Genset Sa Recepteur complexe lsr, activite, clonage, et application au diagnostic, a la prevention et/ou au traitement de d'obesite et des risques ou complications associes
CA2306777A1 (en) * 1997-10-22 1999-04-29 Helix Research Institute Method for screening full-length cdna clones
US20060286639A1 (en) * 1997-11-13 2006-12-21 Serono Genetics Institute S.A. Complementary DNAs encoding proteins with signal peptides
US6548633B1 (en) 1998-12-22 2003-04-15 Genset, S.A. Complementary DNA's encoding proteins with signal peptides
CA2302644A1 (en) * 1997-11-13 1999-05-27 Genset S.A. Extended cdnas for secreted proteins
US6573068B1 (en) 1997-11-13 2003-06-03 Genset, S. A. Claudin-50 protein
EP1903111A3 (en) * 1997-11-13 2008-07-02 Serono Genetics Institute S.A. Extended cDNAs for secreted proteins
DE69841110D1 (de) * 1997-12-17 2009-10-08 Serono Genetics Inst Sa Verlängerte cdns, die für sekretierte proteine kodieren
JP2002502605A (ja) * 1998-02-09 2002-01-29 ジェンセット 分泌タンパク質をコードするｃＤＮＡ
US6783961B1 (en) 1999-02-26 2004-08-31 Genset S.A. Expressed sequence tags and encoded human proteins
WO1999053051A2 (en) * 1998-04-09 1999-10-21 Genset 5' ests and encoded human proteins
US7223727B2 (en) 1998-04-09 2007-05-29 Serono Genetics Institute S.A. GSSP4 polynucleotides and polypeptides and uses thereof
CA2348828C (en) 1998-11-04 2009-09-15 Genset S.A. Genomic and complete cdna sequences of apm1 and biallelic markers thereof
US6455280B1 (en) 1998-12-22 2002-09-24 Genset S.A. Methods and compositions for inhibiting neoplastic cell growth
CA2359757A1 (en) 1999-02-10 2000-08-17 Genset S.A. Polymorphic markers of the lsr gene
EP1586645A3 (en) 1999-02-25 2006-02-22 Ceres Incorporated Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
AU3763200A (en) * 1999-03-19 2000-10-09 Genetics Institute Inc. Primers-attached vector elongation (pave): a 5'-directed cdna cloning strategy
JP2002539784A (ja) * 1999-03-19 2002-11-26 インヴィトロゲン・コーポレーション 大腸菌トポイソメラーゼｉｉｉ及びその相同体を使用して全長核酸配列を得る方法
US7485715B2 (en) 1999-06-18 2009-02-03 Ceres, Inc. Sequence-determined DNA encoding AP2 domain polypeptides
US7479555B2 (en) 1999-07-21 2009-01-20 Ceres, Inc. Polynucleotides having a nucleotide sequence that encodes a polypeptide having MOV34 family activity
CA2311201A1 (en) 1999-08-05 2001-02-05 Genset S.A. Ests and encoded human proteins
US7338787B2 (en) 2000-01-14 2008-03-04 Serono Genetics Institute S.A. Nucleic acids encoding OBG3 globular head and uses thereof
US6566332B2 (en) 2000-01-14 2003-05-20 Genset S.A. OBG3 globular head and uses thereof for decreasing body mass
US20020058617A1 (en) 2000-01-14 2002-05-16 Joachim Fruebis OBG3 globular head and uses thereof for decreasing body mass
US6989367B2 (en) 2000-01-14 2006-01-24 Genset S.A. OBG3 globular head and uses thereof
JP2003524422A (ja) 2000-02-25 2003-08-19 インヴィトロジェン コーポレーション トポイソメラーゼリンカー介在型増幅法
US6387624B1 (en) * 2000-04-14 2002-05-14 Incyte Pharmaceuticals, Inc. Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP1313851B1 (en) 2000-08-21 2006-01-04 Invitrogen Corporation Methods and reagents for molecular cloning
US6875907B2 (en) 2000-09-13 2005-04-05 Pioneer Hi-Bred International, Inc. Antimicrobial peptides and methods of use
AU2002248173B2 (en) 2000-12-08 2007-04-26 Invitrogen Corporation Compositions and methods for rapidly generating recombinant nucleic acid molecules
US20060008817A1 (en) 2000-12-08 2006-01-12 Invitrogen Corporation Methods and compositions for generating recombinant nucleic acid molecules
EP1437936A4 (en) 2001-09-27 2005-10-12 Pioneer Hi Bred Int PHYTATE POLYNUCLEOTIDES AND METHOD FOR THEIR USE
US7358419B2 (en) 2002-04-08 2008-04-15 Pioneer Hi-Bred International, Inc. Enhanced silk exsertion under stress
US20050250100A1 (en) * 2002-06-12 2005-11-10 Yoshihide Hayashizaki Method of utilizing the 5'end of transcribed nucleic acid regions for cloning and analysis
WO2004087916A1 (ja) * 2003-03-28 2004-10-14 Japan As Represented By Director General Of National Rehabilitation Center For Persons With Disabilities ｃＤＮＡ合成方法
DE602004027538D1 (de) 2003-12-01 2010-07-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
GB0417887D0 (en) 2004-08-11 2004-09-15 Ares Trading Sa Protein
US8206924B2 (en) * 2006-09-11 2012-06-26 Osaka University Trace mRNA amplification method and use thereof
BRPI0819743A2 (pt) 2007-11-20 2014-10-07 Pioneer Hi Bred Int Ácido nucléico isolado, cassete de expressão, célula hospedeira, planta transgênica, semente transgênica, método para modular a resposta de etileno em uma planta, proteína isolada
US20100035249A1 (en) * 2008-08-05 2010-02-11 Kabushiki Kaisha Dnaform Rna sequencing and analysis using solid support
US8716553B2 (en) 2009-03-02 2014-05-06 Pioneer Hi Bred International Inc NAC transcriptional activators involved in abiotic stress tolerance
WO2011017492A2 (en) 2009-08-05 2011-02-10 Pioneer Hi-Bred International, Inc. Novel eto1 genes and use of same for reduced ethylene and improved stress tolerance in plants
WO2012019168A2 (en) 2010-08-06 2012-02-09 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
CA2821992A1 (en) 2010-10-01 2012-04-05 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
CA2831613A1 (en) 2011-03-31 2012-10-04 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
ES2911677T3 (es) 2011-10-03 2022-05-20 Modernatx Inc Nucleósidos, nucleótidos y ácidos nucleicos modificados, y sus usos
US20150082480A1 (en) 2011-10-25 2015-03-19 Pioneer Hi Bred International Inc Methods to alter plant cell wall composition for improved biofuel production and silage digestibility
DE12858350T1 (de) 2011-12-16 2021-10-07 Modernatx, Inc. Modifizierte mrna zusammensetzungen
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US10501512B2 (en) 2012-04-02 2019-12-10 Modernatx, Inc. Modified polynucleotides
EP3501550A1 (en) 2012-04-02 2019-06-26 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins associated with human disease
EP2684954A1 (en) 2012-07-10 2014-01-15 Lexogen GmbH 5´ protection dependent amplification
US9422602B2 (en) * 2012-08-15 2016-08-23 Bio-Rad Laboratories, Inc. Methods and compositions for determining nucleic acid degradation
US9597380B2 (en) 2012-11-26 2017-03-21 Modernatx, Inc. Terminally modified RNA
US10077439B2 (en) 2013-03-15 2018-09-18 Modernatx, Inc. Removal of DNA fragments in mRNA production process
US11377470B2 (en) 2013-03-15 2022-07-05 Modernatx, Inc. Ribonucleic acid purification
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
WO2014144767A1 (en) 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
CA2903297A1 (en) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Modulation of acc deaminase expression
EP2971033B8 (en) 2013-03-15 2019-07-10 ModernaTX, Inc. Manufacturing methods for production of rna transcripts
EP3019619B1 (en) 2013-07-11 2021-08-25 ModernaTX, Inc. Compositions comprising synthetic polynucleotides encoding crispr related proteins and synthetic sgrnas and methods of use
US10023626B2 (en) 2013-09-30 2018-07-17 Modernatx, Inc. Polynucleotides encoding immune modulating polypeptides
US10385088B2 (en) 2013-10-02 2019-08-20 Modernatx, Inc. Polynucleotide molecules and uses thereof
EP3052521A1 (en) 2013-10-03 2016-08-10 Moderna Therapeutics, Inc. Polynucleotides encoding low density lipoprotein receptor
US11479766B2 (en) * 2013-12-05 2022-10-25 New England Biolabs, Inc. Methods for labeling a population of RNA molecules
JP2017522028A (ja) 2014-07-16 2017-08-10 モデルナティエックス インコーポレイテッドＭｏｄｅｒｎａＴＸ，Ｉｎｃ． 環状ポリヌクレオチド
WO2017049286A1 (en) 2015-09-17 2017-03-23 Moderna Therapeutics, Inc. Polynucleotides containing a morpholino linker
WO2017066781A1 (en) 2015-10-16 2017-04-20 Modernatx, Inc. Mrna cap analogs with modified phosphate linkage
US11866754B2 (en) 2015-10-16 2024-01-09 Modernatx, Inc. Trinucleotide mRNA cap analogs
JP6417603B2 (ja) 2016-01-27 2018-11-07 株式会社ダナフォーム Ｒｎａの末端領域に対応する核酸の塩基配列を解読する方法およびｄｎａエレメントの分析方法
EP3512944B1 (en) 2016-09-14 2022-07-06 ModernaTX, Inc. High purity rna compositions and methods for preparation thereof
US11912982B2 (en) 2017-08-18 2024-02-27 Modernatx, Inc. Methods for HPLC analysis
MA49914A (fr) 2017-08-18 2021-04-21 Modernatx Inc Procédés analytiques par hplc
WO2023127774A1 (ja) * 2021-12-28 2023-07-06 株式会社ダナフォーム 遺伝子包含体の検出方法及び検出用キット
WO2023237703A1 (en) 2022-06-09 2023-12-14 Vestlandets Innovasjonsselskap As Method of characterizing capped rna

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4661450A (en) * 1983-05-03 1987-04-28 Molecular Genetics Research And Development Limited Partnership Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides
US4710566A (en) * 1984-12-27 1987-12-01 Yale University Purification of the messenger RNA cap-binding protein
US5273879A (en) * 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US5219989A (en) * 1988-12-13 1993-06-15 Mcgill University Bifunctional protein for the isolation of capped mRNA
CA2005252C (en) * 1988-12-13 2001-07-17 Nahum Sonenberg Isolation of full-length cdna clones and capped mrna
US5324829A (en) * 1988-12-16 1994-06-28 Ortho Diagnostic Systems, Inc. High specific activity nucleic acid probes having target recognition and signal generating moieties
JP3315439B2 (ja) * 1992-07-21 2002-08-19 住友電気工業株式会社 細胞特異的ｃＤＮＡの分離法及びライブラリー作製法
JP3337748B2 (ja) * 1992-09-25 2002-10-21 財団法人神奈川科学技術アカデミー 完全長ｃＤＮＡの合成方法、その中間体の製造方法及び完全長ｃＤＮＡを含む組換えベクターの製造方法

Also Published As

Publication number Publication date
DE69624695T2 (de) 2003-07-31
AU5982996A (en) 1996-11-21
US6022715A (en) 2000-02-08
FR2733762A1 (fr) 1996-11-08
CA2220045C (fr) 2001-10-30
ATE227346T1 (de) 2002-11-15
DE69624695D1 (de) 2002-12-12
FR2733762B1 (fr) 1997-08-01
AU719217B2 (en) 2000-05-04
CA2220045A1 (fr) 1996-11-07
WO1996034981A3 (fr) 1997-01-03
DK0824598T3 (da) 2003-03-10
EP0824598B1 (fr) 2002-11-06
WO1996034981A2 (fr) 1996-11-07
ES2186778T3 (es) 2003-05-16
JPH11510364A (ja) 1999-09-14
EP0824598A2 (fr) 1998-02-25

Similar Documents

Publication Publication Date Title
PT824598E (pt) Processo para o acoplamento especifico de um grupo de bloqueio da extremidade 5' de um fragmento de arnm e preparacao do arnm e do adnc completo
BR9609837A (pt) Processo para purificação de vírus por cromatografia
PT835257E (pt) Intermediarios e processo para o fabrico de derivados camptotecina (cpt-11) e compostos relacionados
DE50102614D1 (de) Verfahren zur herstellung von aluminosilicatgläsern
ATE189482T1 (de) Verfahren und vorrichtung für die fragmentation von biomaterialien
ATE242363T1 (de) Brechbarer pfostenverbinder
SE9502921D0 (sv) New compounds
BR9911137A (pt) Processo para tratamento de correntes de fluido
BR0007610A (pt) Método para preparação de cloridrato del-fenilefrina
TR199501122A2 (tr) Etil alkol üretim yöntemi.
FR2659709B1 (fr) Manchon elastique anti-vibratoire notamment pour accouplement d'echappement, et procede de realisation d'un manchon elastique anti-vibratoire.
DE60124289D1 (de) Verfahren zur herstellung von 21-hydroxy-6,19-oxidoprogesteron (21oh-6op)
DK0840792T3 (da) (S)-hydroxynitrillyase fra Hevea brasiliensis
DE69830079D1 (de) Verfahren zur verringerung des aox anteils in nassfesten harzen durch behandlung mit base
NO971723L (no) Fremgangsmåte ved rensning av vandige, tertiære aminoksydopplösninger
FR2782176B1 (fr) Utilisation d'un alcoxy-n-hydroxyalkyl-alcanamide, en tant qu'agent d'elimination de reserve, composition pour eliminer la reserve et procede pour leur preparation et procede pour eliminer la reserve les utilisant
BR9502692A (pt) Processo de refino de descarbonetação de aço fundido contendo cr.
BR9712773A (pt) Processo para a sìntese de benzotiofenos
ATE308571T1 (de) Verfahren zur herstellung von hydrierten aromatischen polymerzusammensetzungen
DE60003807D1 (de) Enantiomere von mercaptolactonen und verfahren zu ihrer herstellung
ATE205473T1 (de) Neue borneolderivate, verfahren zu ihrer herstellung und ihre pharmazeutische verwendung
MA26450A1 (fr) Dimesylate de cis-1-(3-ethoxyphenyl)-1-(4-phenyl-piperazine-1-yl)-4-methylcyclohexane, procede pour sa preparation et composition pharmaceutique.
MX9703563A (es) Metodo analitico para el tratamiento de la saliva.
PT937094E (pt) Processo para a preparacao de ivermectina
DK0746325T3 (da) Kaloriefattig lakserende gelé på basis af flydende paraffinolie samt lactulose, og fremgangsmåde til fremstilling heraf