DK0550696T3 - Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus - Google Patents

Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus

Info

Publication number
DK0550696T3
DK0550696T3 DK91920000T DK91920000T DK0550696T3 DK 0550696 T3 DK0550696 T3 DK 0550696T3 DK 91920000 T DK91920000 T DK 91920000T DK 91920000 T DK91920000 T DK 91920000T DK 0550696 T3 DK0550696 T3 DK 0550696T3
Authority
DK
Denmark
Prior art keywords
pct
nucleic acid
enzyme
thermosipho africanus
purified thermostable
Prior art date
Application number
DK91920000T
Other languages
Danish (da)
English (en)
Inventor
David H Gelfand
Frances C Lawyer
Richard D Abramson
I Lawrence Greenfield
Fred L Reichert
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK0550696T3 publication Critical patent/DK0550696T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Beans For Foods Or Fodder (AREA)
  • Feed For Specific Animals (AREA)
DK91920000T 1990-09-28 1991-09-26 Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus DK0550696T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US59049090A 1990-09-28 1990-09-28
PCT/US1991/007076 WO1992006202A1 (en) 1990-09-28 1991-09-26 PURIFIED THERMOSTABLE NUCLEIC ACID POLYMERASE ENZYME FROM $i(THERMOSIPHO AFRICANUS)

Publications (1)

Publication Number Publication Date
DK0550696T3 true DK0550696T3 (da) 2001-03-19

Family

ID=24362481

Family Applications (1)

Application Number Title Priority Date Filing Date
DK91920000T DK0550696T3 (da) 1990-09-28 1991-09-26 Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus

Country Status (10)

Country Link
US (1) US5968799A (de)
EP (1) EP0550696B1 (de)
JP (1) JP2582980B2 (de)
AT (1) ATE197718T1 (de)
AU (1) AU658378B2 (de)
CA (1) CA2092317A1 (de)
DE (1) DE69132477T2 (de)
DK (1) DK0550696T3 (de)
ES (1) ES2152923T3 (de)
WO (1) WO1992006202A1 (de)

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US6100078A (en) * 1994-04-01 2000-08-08 Gen-Probe Incorporated Purified DNA polymerase from bacillus stearothermophilus ATCC 12980
US5939257A (en) * 1994-04-18 1999-08-17 Amersham Life Science, Inc. Thermostable alkaline phosphatases
WO1995030756A1 (en) * 1994-04-18 1995-11-16 United States Biochemical Corporation Thermostable alkaline phosphatase of thermus thermophilus
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
JP2000502882A (ja) * 1995-09-08 2000-03-14 ライフ・テクノロジーズ・インコーポレイテッド サーモトガ由来のクローン化ｄｎａポリメラーゼ類およびそれらの変異体
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US5861295A (en) 1997-01-02 1999-01-19 Life Technologies, Inc. Nucleic acid-free thermostable enzymes and methods of production thereof
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
WO1999014318A1 (en) * 1997-09-16 1999-03-25 Board Of Regents, The University Of Texas System Method for the complete chemical synthesis and assembly of genes and genomes
US6670127B2 (en) 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US7179590B2 (en) * 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
MXPA03006344A (es) * 2001-01-19 2004-12-03 Egea Biosciences Inc Montaje dirigido por computadora de un polinucleotido que codifica un polipeptido objetivo.
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US20030180741A1 (en) 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
ES2524048T3 (es) 2003-03-31 2014-12-03 F. Hoffmann-La Roche Ag Composiciones y métodos para la detección de ciertos flavivirus, incluidos los miembros del serogrupo del virus de la encefalitis japonesa
WO2004094986A2 (en) 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
EP1664347A1 (de) * 2003-09-23 2006-06-07 University of Missouri Verfahren fur die polynukleotidsynthese mittels thermostabiler enzyme
WO2005054438A2 (en) 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
WO2006029184A2 (en) 2004-09-08 2006-03-16 Expression Diagnostics, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
WO2008021431A2 (en) 2006-08-14 2008-02-21 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
EP2102367A2 (de) 2006-11-09 2009-09-23 XDX, Inc. Verfahren zur diagnose und überwachung des status von systemischem lupus erythematodes
US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
WO2012139748A1 (en) 2011-04-11 2012-10-18 Roche Diagnostics Gmbh Dna polymerases with improved activity
WO2012146260A1 (de) 2011-04-23 2012-11-01 Biolytix Ag Herstellung und verwendung von proteinen in der molekularbiologie
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
CN106518696B (zh) 2011-06-08 2019-06-14 生命技术公司 用于pcr系统的新型去污剂的设计和开发
ES2569723T3 (es) 2011-12-08 2016-05-12 F. Hoffmann-La Roche Ag Polimerasas de DNA con actividad mejorada
EP2788480B1 (de) 2011-12-08 2019-01-16 Roche Diagnostics GmbH Dna-polymerasen mit verbesserter aktivität
WO2013083264A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
JP6742238B2 (ja) 2013-10-25 2020-08-19 ライフ テクノロジーズ コーポレーション Ｐｃｒシステムにおいて使用するための新規化合物及びその用途
CN112760305B (zh) * 2021-01-25 2022-04-29 浙江工业大学 一种栖热腔菌磷酸酶突变体及其应用

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US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
ZA876216B (en) * 1986-08-22 1989-04-26 Cetus Corp Purified thermostable enzyme
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
IL88923A (en) * 1988-01-12 1995-07-31 Hoffmann La Roche Gene encoding a thermostable dna polymerase from thermus aquaticus said dna polymerase and its purification
US5108892A (en) * 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
US5210036A (en) * 1990-04-26 1993-05-11 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
EP0894860B1 (de) * 1990-09-28 2012-12-12 F. Hoffmann-La Roche AG Thermostabile DNA Polymerasen und deren Deletionsmutanten

Also Published As

Publication number Publication date
ES2152923T3 (es) 2001-02-16
EP0550696A1 (de) 1993-07-14
DE69132477D1 (en) 2000-12-28
US5968799A (en) 1999-10-19
JPH06504196A (ja) 1994-05-19
AU8907791A (en) 1992-04-28
CA2092317A1 (en) 1992-03-29
EP0550696B1 (de) 2000-11-22
AU658378B2 (en) 1995-04-13
WO1992006202A1 (en) 1992-04-16
DE69132477T2 (de) 2001-06-13
ATE197718T1 (de) 2000-12-15
JP2582980B2 (ja) 1997-02-19

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