DE69930765T2 - Methode zur kontrollierten verlängerung eines oligonukleotids - Google Patents
Methode zur kontrollierten verlängerung eines oligonukleotids Download PDFInfo
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- DE69930765T2 DE69930765T2 DE69930765T DE69930765T DE69930765T2 DE 69930765 T2 DE69930765 T2 DE 69930765T2 DE 69930765 T DE69930765 T DE 69930765T DE 69930765 T DE69930765 T DE 69930765T DE 69930765 T2 DE69930765 T2 DE 69930765T2
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- primer
- oligonucleotide
- extended
- sequence
- modified
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US233413 | 1999-01-19 | ||
| US09/233,413 US6200757B1 (en) | 1999-01-19 | 1999-01-19 | Method for controlling the extension of an oligonucleotide |
| PCT/US1999/029253 WO2000043544A1 (en) | 1999-01-19 | 1999-12-10 | Method for controlling the extension of an oligonucleotide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69930765D1 DE69930765D1 (de) | 2006-05-18 |
| DE69930765T2 true DE69930765T2 (de) | 2006-11-23 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69930765T Expired - Lifetime DE69930765T2 (de) | 1999-01-19 | 1999-12-10 | Methode zur kontrollierten verlängerung eines oligonukleotids |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6200757B1 (https=) |
| EP (1) | EP1147230B1 (https=) |
| JP (1) | JP4657454B2 (https=) |
| AT (1) | ATE322557T1 (https=) |
| CA (1) | CA2358992C (https=) |
| DE (1) | DE69930765T2 (https=) |
| HK (1) | HK1042324B (https=) |
| WO (1) | WO2000043544A1 (https=) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6274353B1 (en) | 1999-09-22 | 2001-08-14 | Genecopoeia, Inc. | Method and compositions for improved polynucleotide synthesis |
| US7033763B2 (en) * | 2000-02-23 | 2006-04-25 | City Of Hope | Pyrophosphorolysis activated polymerization (PAP) |
| AU2001241621B2 (en) * | 2000-02-23 | 2005-08-25 | City Of Hope | Pyrophosphorolysis activated polymerization (PAP): application to allele-specific amplification and nucleic acid sequence determination |
| EP1275735A1 (en) * | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Composition and method for hot start nucleic acid amplification |
| US6667165B2 (en) | 2001-11-13 | 2003-12-23 | Eppendorf Ag | Method and compositions for reversible inhibition of thermostable polymerases |
| US7211382B2 (en) * | 2002-04-09 | 2007-05-01 | Orchid Cellmark Inc. | Primer extension using modified nucleotides |
| WO2004090153A2 (en) * | 2003-04-01 | 2004-10-21 | Eragen Biosciences,Inc. | Polymerase inhibitor and method of using same |
| US8293472B2 (en) * | 2005-06-07 | 2012-10-23 | Luminex Corporation | Methods for detection and typing of nucleic acids |
| US20070048758A1 (en) * | 2005-06-09 | 2007-03-01 | Epoch Biosciences, Inc. | Improved primer-based amplification methods |
| AU2006287548A1 (en) * | 2005-09-06 | 2007-03-15 | Gen-Probe Incorporated | Methods, compositions and kits for isothermal amplification of nucleic acids |
| ES2357746T3 (es) * | 2006-03-28 | 2011-04-29 | Commonwealth Scientific And Industrial Research Organisation | Amplificación de fragmentos de adn. |
| DE102008005667A1 (de) | 2008-01-19 | 2009-07-30 | Olfert Landt | Basenmodifizierte Primeroligomere für die Multiplex-RT-PCR |
| ES2527653T3 (es) * | 2008-10-20 | 2015-01-28 | F. Hoffmann-La Roche Ag | Amplificación específica de alelo usando un cebador con un nucleótido modificado |
| CA2777446A1 (en) * | 2009-10-13 | 2011-04-21 | Syntezza Molecular Detection Israel Ltd. | Methods and compositions for amplifying target sequences from nucleic acid samples |
| US9238832B2 (en) | 2009-12-11 | 2016-01-19 | Roche Molecular Systems, Inc. | Allele-specific amplification of nucleic acids |
| US8614071B2 (en) | 2009-12-11 | 2013-12-24 | Roche Molecular Systems, Inc. | Preferential amplification of mRNA over DNA using chemically modified primers |
| DK3109326T3 (da) | 2010-06-21 | 2022-02-14 | Life Technologies Corp | Sammensætning og fremgangsmåde til nukleinsyresyntese og amplifikation under anvendelse af rt |
| EP2582850B1 (en) | 2010-06-21 | 2016-11-09 | Life Technologies Corporation | Compositions, kits, and methods for synthesis and/or detection of nucleic acids |
| US9115394B2 (en) | 2011-12-22 | 2015-08-25 | Roche Molecular Systems, Inc. | Methods and reagents for reducing non-specific amplification |
| EP4026912A1 (en) | 2012-05-10 | 2022-07-13 | The General Hospital Corporation | Methods for determining a nucleotide sequence |
| JP7016215B2 (ja) | 2014-01-27 | 2022-02-04 | ザ ジェネラル ホスピタル コーポレイション | 配列決定のために核酸を調製する方法 |
| US9909169B2 (en) | 2014-12-17 | 2018-03-06 | Roche Molecular Systems, Inc. | Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression |
| WO2018053362A1 (en) | 2016-09-15 | 2018-03-22 | ArcherDX, Inc. | Methods of nucleic acid sample preparation |
| CA3037190A1 (en) | 2016-09-15 | 2018-03-22 | ArcherDX, Inc. | Methods of nucleic acid sample preparation for analysis of cell-free dna |
| WO2019231568A1 (en) * | 2018-05-31 | 2019-12-05 | Omniome, Inc. | Increased signal to noise in nucleic acid sequencing |
| US11180794B2 (en) | 2018-05-31 | 2021-11-23 | Omniome, Inc. | Methods and compositions for capping nucleic acids |
| WO2025007089A1 (en) | 2023-06-30 | 2025-01-02 | Life Technologies Corporation | Compositions, methods and kits for nucleic acids synthesis and amplification |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US5508178A (en) | 1989-01-19 | 1996-04-16 | Rose; Samuel | Nucleic acid amplification using single primer |
| ATE142272T1 (de) * | 1989-01-19 | 1996-09-15 | Behringwerke Ag | Nukleinsäure-amplifikation unter verwendung eines einzelprimers |
| US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
| DE69118930T2 (de) | 1990-01-26 | 1997-01-09 | Abbott Lab | Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion |
| DE69131891T2 (de) | 1990-02-16 | 2000-06-15 | F. Hoffmann-La Roche Ag, Basel | Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion |
| US5348853A (en) | 1991-12-16 | 1994-09-20 | Biotronics Corporation | Method for reducing non-specific priming in DNA amplification |
| US5338671A (en) * | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| US5565339A (en) | 1992-10-08 | 1996-10-15 | Hoffmann-La Roche Inc. | Compositions and methods for inhibiting dimerization of primers during storage of polymerase chain reaction reagents |
| US5599660A (en) | 1993-01-19 | 1997-02-04 | Pharmacia Biotech Inc. | Method and preparation for sequential delivery of wax-embedded, inactivated biological and chemical reagents |
| FR2722799B1 (fr) * | 1994-07-21 | 1996-10-04 | Parteurop | Procede d'amplification d'acide nucleique a l'aide d'un nucleoside modifie, et detection du produit d'amplification a l'aide d'anticorps |
| US5705366A (en) | 1994-09-15 | 1998-01-06 | Johnson & Johnson Clinical Diagnostics, Inc. | Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor |
| AU1565397A (en) * | 1995-12-22 | 1997-07-17 | Behringwerke Aktiengesellschaft | Detection of differences in nucleic acids |
| CA2217143A1 (en) * | 1996-02-01 | 1997-08-07 | Behringwerke Aktiengesellschaft | Positive controls for use in polynucleotide amplification |
| JP3545158B2 (ja) * | 1996-03-14 | 2004-07-21 | シスメックス株式会社 | 遺伝子検出法 |
| US6482590B1 (en) * | 1996-12-20 | 2002-11-19 | Aventis Behring Gmbh | Method for polynucleotide amplification |
| ATE280177T1 (de) * | 1997-03-20 | 2004-11-15 | Hoffmann La Roche | Modifizierte primer |
-
1999
- 1999-01-19 US US09/233,413 patent/US6200757B1/en not_active Expired - Lifetime
- 1999-12-10 JP JP2000594952A patent/JP4657454B2/ja not_active Expired - Fee Related
- 1999-12-10 WO PCT/US1999/029253 patent/WO2000043544A1/en not_active Ceased
- 1999-12-10 AT AT99965197T patent/ATE322557T1/de not_active IP Right Cessation
- 1999-12-10 EP EP99965197A patent/EP1147230B1/en not_active Expired - Lifetime
- 1999-12-10 DE DE69930765T patent/DE69930765T2/de not_active Expired - Lifetime
- 1999-12-10 CA CA2358992A patent/CA2358992C/en not_active Expired - Fee Related
- 1999-12-10 HK HK02102877.2A patent/HK1042324B/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| JP2002534986A (ja) | 2002-10-22 |
| CA2358992C (en) | 2010-10-12 |
| EP1147230A1 (en) | 2001-10-24 |
| ATE322557T1 (de) | 2006-04-15 |
| CA2358992A1 (en) | 2000-07-27 |
| JP4657454B2 (ja) | 2011-03-23 |
| US6200757B1 (en) | 2001-03-13 |
| HK1042324A1 (en) | 2002-08-09 |
| DE69930765D1 (de) | 2006-05-18 |
| WO2000043544A1 (en) | 2000-07-27 |
| HK1042324B (en) | 2006-06-23 |
| EP1147230B1 (en) | 2006-04-05 |
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