DE69835957T2 - Methoden zur isolation von proteinaseinhibitor proteinen von kartoffel knollen - Google Patents
Methoden zur isolation von proteinaseinhibitor proteinen von kartoffel knollen Download PDFInfo
- Publication number
- DE69835957T2 DE69835957T2 DE69835957T DE69835957T DE69835957T2 DE 69835957 T2 DE69835957 T2 DE 69835957T2 DE 69835957 T DE69835957 T DE 69835957T DE 69835957 T DE69835957 T DE 69835957T DE 69835957 T2 DE69835957 T2 DE 69835957T2
- Authority
- DE
- Germany
- Prior art keywords
- protease inhibitor
- extract
- dialysis
- temperature
- proteins
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000137 peptide hydrolase inhibitor Substances 0.000 title claims abstract description 76
- 238000000034 method Methods 0.000 title claims abstract description 46
- 244000061456 Solanum tuberosum Species 0.000 title claims abstract description 45
- 235000002595 Solanum tuberosum Nutrition 0.000 title claims abstract description 45
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 32
- 102000004169 proteins and genes Human genes 0.000 title abstract description 30
- 238000002955 isolation Methods 0.000 title description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 76
- 239000000284 extract Substances 0.000 claims abstract description 39
- 108091006086 inhibitor proteins Proteins 0.000 claims abstract description 38
- 238000000502 dialysis Methods 0.000 claims abstract description 29
- 239000000203 mixture Substances 0.000 claims abstract description 26
- 239000002244 precipitate Substances 0.000 claims abstract description 23
- 241000196324 Embryophyta Species 0.000 claims abstract description 19
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 48
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 claims description 41
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 24
- 235000019253 formic acid Nutrition 0.000 claims description 24
- 101001033883 Cenchritis muricatus Protease inhibitor 2 Proteins 0.000 claims description 17
- 108090000631 Trypsin Proteins 0.000 claims description 12
- 102000004142 Trypsin Human genes 0.000 claims description 12
- 230000000694 effects Effects 0.000 claims description 12
- 239000012588 trypsin Substances 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 10
- 235000012015 potatoes Nutrition 0.000 claims description 10
- 238000001556 precipitation Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000001816 cooling Methods 0.000 claims description 7
- 239000002904 solvent Substances 0.000 claims description 7
- 239000000385 dialysis solution Substances 0.000 claims description 6
- 230000001476 alcoholic effect Effects 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 239000002753 trypsin inhibitor Substances 0.000 claims description 5
- 239000008399 tap water Substances 0.000 claims description 4
- 235000020679 tap water Nutrition 0.000 claims description 4
- 101710162629 Trypsin inhibitor Proteins 0.000 claims description 3
- 244000017020 Ipomoea batatas Species 0.000 claims description 2
- 235000002678 Ipomoea batatas Nutrition 0.000 claims description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 2
- 240000003183 Manihot esculenta Species 0.000 claims description 2
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 claims description 2
- 240000003768 Solanum lycopersicum Species 0.000 claims description 2
- 230000002378 acidificating effect Effects 0.000 claims description 2
- 235000013399 edible fruits Nutrition 0.000 claims description 2
- 235000003869 genetically modified organism Nutrition 0.000 claims description 2
- 230000002401 inhibitory effect Effects 0.000 claims description 2
- 235000021374 legumes Nutrition 0.000 claims description 2
- 239000012528 membrane Substances 0.000 claims description 2
- 239000000287 crude extract Substances 0.000 claims 2
- 101710163806 Trypsin/chymotrypsin inhibitor Proteins 0.000 claims 1
- 229940043292 chymotrypsin / trypsin Drugs 0.000 claims 1
- 239000000356 contaminant Substances 0.000 claims 1
- 230000001376 precipitating effect Effects 0.000 claims 1
- 238000000605 extraction Methods 0.000 abstract description 13
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 31
- 239000003112 inhibitor Substances 0.000 description 22
- 239000000463 material Substances 0.000 description 15
- 108090000317 Chymotrypsin Proteins 0.000 description 14
- 229960002376 chymotrypsin Drugs 0.000 description 14
- 239000000243 solution Substances 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 6
- 238000004925 denaturation Methods 0.000 description 6
- 230000036425 denaturation Effects 0.000 description 6
- 238000004128 high performance liquid chromatography Methods 0.000 description 6
- 230000003389 potentiating effect Effects 0.000 description 6
- 239000007788 liquid Substances 0.000 description 5
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 238000001502 gel electrophoresis Methods 0.000 description 4
- 230000002427 irreversible effect Effects 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 150000001298 alcohols Chemical class 0.000 description 3
- 238000012870 ammonium sulfate precipitation Methods 0.000 description 3
- 238000000265 homogenisation Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 2
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 229940122618 Trypsin inhibitor Drugs 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 2
- 239000001099 ammonium carbonate Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 108091007735 digestive proteases Proteins 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000007717 exclusion Effects 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 241000257303 Hymenoptera Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241000208292 Solanaceae Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000012675 alcoholic extract Substances 0.000 description 1
- 238000003277 amino acid sequence analysis Methods 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 238000011210 chromatographic step Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 229940075933 dithionate Drugs 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 230000002431 foraging effect Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000003505 heat denaturation Methods 0.000 description 1
- 244000038280 herbivores Species 0.000 description 1
- 230000037125 natural defense Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 239000002195 soluble material Substances 0.000 description 1
- 238000002943 spectrophotometric absorbance Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8114—Kunitz type inhibitors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US5159897P | 1997-07-02 | 1997-07-02 | |
| US51598P | 1997-07-02 | ||
| PCT/US1998/013832 WO1999001474A1 (en) | 1997-07-02 | 1998-07-01 | Methods for the isolation of proteinase inhibitor proteins from potato tubers |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69835957D1 DE69835957D1 (de) | 2006-11-02 |
| DE69835957T2 true DE69835957T2 (de) | 2007-05-24 |
Family
ID=21972275
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69835957T Expired - Lifetime DE69835957T2 (de) | 1997-07-02 | 1998-07-01 | Methoden zur isolation von proteinaseinhibitor proteinen von kartoffel knollen |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US6414124B1 (https=) |
| EP (1) | EP1003785B1 (https=) |
| JP (1) | JP4416188B2 (https=) |
| AT (1) | ATE340191T1 (https=) |
| AU (1) | AU738102B2 (https=) |
| CA (1) | CA2294613A1 (https=) |
| DE (1) | DE69835957T2 (https=) |
| ES (1) | ES2273422T3 (https=) |
| PT (1) | PT1003785E (https=) |
| WO (1) | WO1999001474A1 (https=) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7507542B2 (en) | 2001-05-08 | 2009-03-24 | Ucb Sa | Method for regulating immune function using the FOXP3 protein |
| US8377877B2 (en) * | 2001-07-06 | 2013-02-19 | Kemin Foods, L.C. | Composition and method for reducing post-prandial blood glucose |
| US6686456B2 (en) | 2001-07-06 | 2004-02-03 | Kemin Foods, L.C. | Method for the elimination of Kunitz and Bowman-Birk trypsin inhibitors and carboxypeptidase inhibitor from potato proteins |
| US20030077265A1 (en) * | 2001-07-06 | 2003-04-24 | Rod Ausich | Isolation and purification of proteinase inhibitor ll |
| WO2003003837A1 (en) * | 2001-07-06 | 2003-01-16 | Kemin Foods, L.C. | Isolation and purification of proteinase inhibitor ii |
| US7371418B2 (en) | 2001-07-06 | 2008-05-13 | Sheabar Fayad Z | Method for controlling the yield and purity of proteinase inhibitor II during extraction |
| US6872544B2 (en) | 2001-09-25 | 2005-03-29 | Kemin Consumer Care, L.C. | Raw material selection and analysis for the isolation of proteinase inhibitor II from whole potatoes |
| KR100568607B1 (ko) * | 2002-07-22 | 2006-04-07 | 김송배 | 헤데라게닌3-Ｏ-α-Ｌ-람노피라노실(1→2)-[β-Ｄ-글루코피라노실(1→4)]-α-Ｌ-아라비노피라노사이드 또는 그를 함유하는백두옹 추출물의 고형암 치료제로서의 용도 |
| US20060035827A1 (en) * | 2004-06-24 | 2006-02-16 | Green Gary M | Compositions and methods for the treatment or prevention of gallbladder disease |
| KR100654232B1 (ko) | 2004-11-23 | 2006-12-05 | (주)포테이토밸리 | 골든밸리 품종의 감자로부터 분리된 열안정성 펩타이드 및상기 펩타이드를 유효성분으로 함유하는 기능성 소재로의용도 |
| US20070148267A1 (en) * | 2005-12-15 | 2007-06-28 | Svyatoslav Komarnytskyy | Method for producing plant extracts enriched with protease inhibitors for regulation of appetite and food intake in mammals |
| GB0622136D0 (en) * | 2006-11-03 | 2006-12-20 | Univ Manchester | Treatment of gastrointestinal diseases |
| GB0622137D0 (en) * | 2006-11-03 | 2006-12-20 | Univ Manchester | Treatment of gastrointestinal diseases |
| CA3055986C (en) | 2017-03-31 | 2025-09-16 | J.R. Simplot Company | POTATO PROTEIN POWDERS |
| US20210360943A1 (en) * | 2018-05-07 | 2021-11-25 | LIHME PROTEIN SOLUTIONS ApS | Integrated precipitation and membrane filtration processes for isolation of potato proteins |
| CN114957505B (zh) * | 2022-07-05 | 2023-03-28 | 山东省分析测试中心 | 一种多糖类胰蛋白酶抑制剂及其制备方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NO905003D0 (no) * | 1990-11-19 | 1990-11-19 | Norsk Potetindustrier Al | Fremgangsmaate for fremstilling av proteaseinhibitorer frapotetsaft. |
| DK91092D0 (https=) * | 1992-07-10 | 1992-07-10 | Novo Nordisk As |
-
1998
- 1998-07-01 ES ES98930519T patent/ES2273422T3/es not_active Expired - Lifetime
- 1998-07-01 AU AU79889/98A patent/AU738102B2/en not_active Ceased
- 1998-07-01 DE DE69835957T patent/DE69835957T2/de not_active Expired - Lifetime
- 1998-07-01 EP EP98930519A patent/EP1003785B1/en not_active Expired - Lifetime
- 1998-07-01 CA CA002294613A patent/CA2294613A1/en not_active Abandoned
- 1998-07-01 AT AT98930519T patent/ATE340191T1/de active
- 1998-07-01 WO PCT/US1998/013832 patent/WO1999001474A1/en not_active Ceased
- 1998-07-01 PT PT98930519T patent/PT1003785E/pt unknown
- 1998-07-01 JP JP50740399A patent/JP4416188B2/ja not_active Expired - Lifetime
-
1999
- 1999-12-29 US US09/475,352 patent/US6414124B1/en not_active Expired - Lifetime
-
2002
- 2002-05-07 US US10/140,760 patent/US20020183491A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CA2294613A1 (en) | 1999-01-14 |
| US20020183491A1 (en) | 2002-12-05 |
| AU7988998A (en) | 1999-01-25 |
| JP4416188B2 (ja) | 2010-02-17 |
| US6414124B1 (en) | 2002-07-02 |
| ES2273422T3 (es) | 2007-05-01 |
| WO1999001474A1 (en) | 1999-01-14 |
| PT1003785E (pt) | 2007-01-31 |
| ATE340191T1 (de) | 2006-10-15 |
| AU738102B2 (en) | 2001-09-06 |
| JP2002507995A (ja) | 2002-03-12 |
| EP1003785B1 (en) | 2006-09-20 |
| DE69835957D1 (de) | 2006-11-02 |
| EP1003785A1 (en) | 2000-05-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |