DE69834645T2 - Dendritische nukleinsäuren mit maximaler selbstzusammenlagerung - Google Patents
Dendritische nukleinsäuren mit maximaler selbstzusammenlagerung Download PDFInfo
- Publication number
- DE69834645T2 DE69834645T2 DE69834645T DE69834645T DE69834645T2 DE 69834645 T2 DE69834645 T2 DE 69834645T2 DE 69834645 T DE69834645 T DE 69834645T DE 69834645 T DE69834645 T DE 69834645T DE 69834645 T2 DE69834645 T2 DE 69834645T2
- Authority
- DE
- Germany
- Prior art keywords
- hybridization
- monomers
- nucleic acid
- polynucleotide
- monomer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 125
- 102000039446 nucleic acids Human genes 0.000 title abstract description 119
- 108020004707 nucleic acids Proteins 0.000 title abstract description 119
- 239000000178 monomer Substances 0.000 claims abstract description 149
- 239000002773 nucleotide Substances 0.000 claims abstract description 12
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 12
- 239000011159 matrix material Substances 0.000 claims abstract description 11
- 238000009396 hybridization Methods 0.000 claims description 74
- 108091033319 polynucleotide Proteins 0.000 claims description 34
- 102000040430 polynucleotide Human genes 0.000 claims description 34
- 239000002157 polynucleotide Substances 0.000 claims description 34
- 239000003153 chemical reaction reagent Substances 0.000 claims description 11
- 238000001514 detection method Methods 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 4
- 230000000295 complement effect Effects 0.000 abstract description 21
- 238000001338 self-assembly Methods 0.000 abstract description 4
- 239000000412 dendrimer Substances 0.000 description 88
- 229920000736 dendritic polymer Polymers 0.000 description 88
- 239000010410 layer Substances 0.000 description 64
- 108020004414 DNA Proteins 0.000 description 33
- 238000007792 addition Methods 0.000 description 17
- 239000003999 initiator Substances 0.000 description 16
- 210000001624 hip Anatomy 0.000 description 14
- 238000000034 method Methods 0.000 description 14
- 238000006243 chemical reaction Methods 0.000 description 12
- ZCCUUQDIBDJBTK-UHFFFAOYSA-N psoralen Chemical compound C1=C2OC(=O)C=CC2=CC2=C1OC=C2 ZCCUUQDIBDJBTK-UHFFFAOYSA-N 0.000 description 9
- 229920002521 macromolecule Polymers 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 238000004132 cross linking Methods 0.000 description 5
- 238000013461 design Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 4
- 108091034117 Oligonucleotide Proteins 0.000 description 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 238000002844 melting Methods 0.000 description 4
- 230000008018 melting Effects 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 238000003752 polymerase chain reaction Methods 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- VXGRJERITKFWPL-UHFFFAOYSA-N 4',5'-Dihydropsoralen Natural products C1=C2OC(=O)C=CC2=CC2=C1OCC2 VXGRJERITKFWPL-UHFFFAOYSA-N 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 239000003431 cross linking reagent Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- FMHHVULEAZTJMA-UHFFFAOYSA-N trioxsalen Chemical compound CC1=CC(=O)OC2=C1C=C1C=C(C)OC1=C2C FMHHVULEAZTJMA-UHFFFAOYSA-N 0.000 description 3
- 239000004793 Polystyrene Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000006735 deficit Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000010494 dissociation reaction Methods 0.000 description 2
- 230000005593 dissociations Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000833 heterodimer Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 229960004857 mitomycin Drugs 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 238000004153 renaturation Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 239000002344 surface layer Substances 0.000 description 2
- ZDSRFXVZVHSYMA-CMOCDZPBSA-N (2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-amino-3-(4-hydroxyphenyl)propanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-4-carboxybutanoyl]amino]pentanedioic acid Chemical compound C([C@H](N)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O)C1=CC=C(O)C=C1 ZDSRFXVZVHSYMA-CMOCDZPBSA-N 0.000 description 1
- GZFVOFMKXXTWQE-UHFFFAOYSA-N 3,8-diazido-5-ethyl-6-phenylphenanthridin-5-ium Chemical compound C12=CC(N=[N+]=[N-])=CC=C2C2=CC=C(N=[N+]=[N-])C=C2[N+](CC)=C1C1=CC=CC=C1 GZFVOFMKXXTWQE-UHFFFAOYSA-N 0.000 description 1
- HAWQZPVGCLHOBD-UHFFFAOYSA-N 3-(aminomethyl)-2,5,9-trimethylfuro[3,2-g]chromen-7-one;hydrochloride Chemical compound Cl.O1C(=O)C=C(C)C2=C1C(C)=C1OC(C)=C(CN)C1=C2 HAWQZPVGCLHOBD-UHFFFAOYSA-N 0.000 description 1
- RGJSDHXSAKMPNM-UHFFFAOYSA-N 3-(hydroxymethyl)-2,5,9-trimethylfuro[3,2-g]chromen-7-one Chemical compound O1C(=O)C=C(C)C2=C1C(C)=C1OC(C)=C(CO)C1=C2 RGJSDHXSAKMPNM-UHFFFAOYSA-N 0.000 description 1
- UPAKHQWBZFCLMG-UHFFFAOYSA-N 3-(methoxymethyl)-2,5,9-trimethylfuro[3,2-g]chromen-7-one Chemical compound O1C(=O)C=C(C)C2=C1C(C)=C1OC(C)=C(COC)C1=C2 UPAKHQWBZFCLMG-UHFFFAOYSA-N 0.000 description 1
- STQGQHZAVUOBTE-UHFFFAOYSA-N 7-Cyan-hept-2t-en-4,6-diinsaeure Natural products C1=2C(O)=C3C(=O)C=4C(OC)=CC=CC=4C(=O)C3=C(O)C=2CC(O)(C(C)=O)CC1OC1CC(N)C(O)C(C)O1 STQGQHZAVUOBTE-UHFFFAOYSA-N 0.000 description 1
- BUNGCZLFHHXKBX-UHFFFAOYSA-N 8-methoxypsoralen Natural products C1=CC(=O)OC2=C1C=C1CCOC1=C2OC BUNGCZLFHHXKBX-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 101100268670 Caenorhabditis elegans acc-3 gene Proteins 0.000 description 1
- 102100025570 Cancer/testis antigen 1 Human genes 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- WEAHRLBPCANXCN-UHFFFAOYSA-N Daunomycin Natural products CCC1(O)CC(OC2CC(N)C(O)C(C)O2)c3cc4C(=O)c5c(OC)cccc5C(=O)c4c(O)c3C1 WEAHRLBPCANXCN-UHFFFAOYSA-N 0.000 description 1
- 241000701867 Enterobacteria phage T7 Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 102100036263 Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial Human genes 0.000 description 1
- 101000856237 Homo sapiens Cancer/testis antigen 1 Proteins 0.000 description 1
- 101001001786 Homo sapiens Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial Proteins 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- QXKHYNVANLEOEG-UHFFFAOYSA-N Methoxsalen Chemical compound C1=CC(=O)OC2=C1C=C1C=COC1=C2OC QXKHYNVANLEOEG-UHFFFAOYSA-N 0.000 description 1
- 101100208721 Mus musculus Usp5 gene Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108091081548 Palindromic sequence Proteins 0.000 description 1
- 101100271190 Plasmodium falciparum (isolate 3D7) ATAT gene Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 238000011481 absorbance measurement Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 229940041181 antineoplastic drug Drugs 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- DQLATGHUWYMOKM-UHFFFAOYSA-L cisplatin Chemical compound N[Pt](N)(Cl)Cl DQLATGHUWYMOKM-UHFFFAOYSA-L 0.000 description 1
- 229960004316 cisplatin Drugs 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000002872 contrast media Substances 0.000 description 1
- 229940039231 contrast media Drugs 0.000 description 1
- STQGQHZAVUOBTE-VGBVRHCVSA-N daunorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(C)=O)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 STQGQHZAVUOBTE-VGBVRHCVSA-N 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000009830 intercalation Methods 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000013178 mathematical model Methods 0.000 description 1
- 229960004469 methoxsalen Drugs 0.000 description 1
- SQBBOVROCFXYBN-UHFFFAOYSA-N methoxypsoralen Natural products C1=C2OC(=O)C(OC)=CC2=CC2=C1OC=C2 SQBBOVROCFXYBN-UHFFFAOYSA-N 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 229920000620 organic polymer Polymers 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 101150101567 pat-2 gene Proteins 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 150000003194 psoralenes Chemical class 0.000 description 1
- 239000002719 pyrimidine nucleotide Substances 0.000 description 1
- 150000003230 pyrimidines Chemical class 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012047 saturated solution Substances 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229960000850 trioxysalen Drugs 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- 108010068794 tyrosyl-tyrosyl-glutamyl-glutamic acid Proteins 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US5402097P | 1997-07-29 | 1997-07-29 | |
| US54020P | 1997-07-29 | ||
| PCT/US1998/015764 WO1999006595A1 (en) | 1997-07-29 | 1998-07-29 | Dendritic nucleic acids exhibiting maximal self-assembly |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69834645D1 DE69834645D1 (de) | 2006-06-29 |
| DE69834645T2 true DE69834645T2 (de) | 2007-05-03 |
Family
ID=21988251
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69834645T Expired - Lifetime DE69834645T2 (de) | 1997-07-29 | 1998-07-29 | Dendritische nukleinsäuren mit maximaler selbstzusammenlagerung |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6274723B1 (enExample) |
| EP (1) | EP1005573B1 (enExample) |
| JP (1) | JP4221145B2 (enExample) |
| AT (1) | ATE327344T1 (enExample) |
| AU (1) | AU8601898A (enExample) |
| DE (1) | DE69834645T2 (enExample) |
| ES (1) | ES2270527T3 (enExample) |
| WO (1) | WO1999006595A1 (enExample) |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040266706A1 (en) * | 2002-11-05 | 2004-12-30 | Muthiah Manoharan | Cross-linked oligomeric compounds and their use in gene modulation |
| US5898031A (en) * | 1996-06-06 | 1999-04-27 | Isis Pharmaceuticals, Inc. | Oligoribonucleotides for cleaving RNA |
| US7812149B2 (en) | 1996-06-06 | 2010-10-12 | Isis Pharmaceuticals, Inc. | 2′-Fluoro substituted oligomeric compounds and compositions for use in gene modulations |
| US9096636B2 (en) * | 1996-06-06 | 2015-08-04 | Isis Pharmaceuticals, Inc. | Chimeric oligomeric compounds and their use in gene modulation |
| GB9811403D0 (en) * | 1998-05-27 | 1998-07-22 | Isis Innovation | Polynucleotide multimers and their use in hybridisation assays |
| AU2001247328C1 (en) * | 2000-03-08 | 2006-10-26 | Datascope Investment Corp. | Methods for assay and detection on a microarray |
| DK1188841T3 (da) * | 2000-03-31 | 2009-04-20 | Sanko Junyaku Kk | Probe til fremstilling af en polymerprobe, fremgangsmåde til fremstilling af en polymerprobe samt anvendelse af polymerproben |
| WO2002006511A2 (en) * | 2000-07-19 | 2002-01-24 | Genisphere Inc | Methods for detecting and assaying nucleic acid sequences |
| US7011943B2 (en) * | 2000-09-06 | 2006-03-14 | Transnetyx, Inc. | Method for detecting a designated genetic sequence in murine genomic DNA |
| ES2273892T3 (es) | 2000-10-11 | 2007-05-16 | Sanko Junyaku Co., Ltd. | Procedimiento de construccion de sondas de autoensamblaje y procedimiento de deteccion de las mismas. |
| EP1451350A4 (en) * | 2001-11-05 | 2006-05-10 | Transgenomic Inc | PROCESSES, SYSTEMS AND KITS FOR THE ANALYSIS OF POLYNUCLEOTIDES |
| US20040009495A1 (en) * | 2001-12-07 | 2004-01-15 | Whitehead Institute For Biomedical Research | Methods and products related to drug screening using gene expression patterns |
| US20040023248A1 (en) * | 2001-12-07 | 2004-02-05 | Whitehead Institiute For Biomedical Research | Methods and reagents for improving nucleic acid detection |
| US20030175709A1 (en) * | 2001-12-20 | 2003-09-18 | Murphy George L. | Method and system for depleting rRNA populations |
| WO2004044136A2 (en) | 2002-11-05 | 2004-05-27 | Isis Pharmaceuticals, Inc. | Compositions comprising alternating 2’-modified nucleosides for use in gene modulation |
| US8569474B2 (en) | 2004-03-09 | 2013-10-29 | Isis Pharmaceuticals, Inc. | Double stranded constructs comprising one or more short strands hybridized to a longer strand |
| JP2007537450A (ja) | 2004-05-12 | 2007-12-20 | ナノスフェアー インコーポレイテッド | バイオバーコードに基づく標的検体の検出 |
| US8394947B2 (en) | 2004-06-03 | 2013-03-12 | Isis Pharmaceuticals, Inc. | Positionally modified siRNA constructs |
| US7884086B2 (en) | 2004-09-08 | 2011-02-08 | Isis Pharmaceuticals, Inc. | Conjugates for use in hepatocyte free uptake assays |
| WO2007019444A2 (en) * | 2005-08-05 | 2007-02-15 | Euclid Diagnostics Llc | Subtractive separation and amplification of non-ribosomal transcribed rna (nrrna) |
| US7942056B2 (en) * | 2006-01-23 | 2011-05-17 | Drexel University | Self-exciting, self-sensing piezoelectric cantilever sensor |
| WO2007133619A1 (en) * | 2006-05-10 | 2007-11-22 | Drexel University | Molecular control of surface coverage |
| US20070275388A1 (en) * | 2006-05-25 | 2007-11-29 | Daniel Ryan | Dendrimers that possess a single target annealing site and uses thereof |
| US7892759B2 (en) | 2007-02-16 | 2011-02-22 | Drexel University | Enhanced sensitivity of a cantilever sensor via specific bindings |
| US8512947B2 (en) * | 2007-02-16 | 2013-08-20 | Drexel University | Detection of nucleic acids using a cantilever sensor |
| WO2008147526A1 (en) | 2007-05-23 | 2008-12-04 | The Trustees Of The University Of Pennsylvania | Targeted carriers for intracellular drug delivery |
| US7993854B2 (en) * | 2007-05-30 | 2011-08-09 | Drexel University | Detection and quantification of biomarkers via a piezoelectric cantilever sensor |
| US8236508B2 (en) * | 2008-01-29 | 2012-08-07 | Drexel University | Detecting and measuring live pathogens utilizing a mass detection device |
| WO2010017544A2 (en) | 2008-08-08 | 2010-02-11 | Genisphere, Inc. | Long-acting dna dendrimers and methods thereof |
| US20110206611A1 (en) | 2010-02-24 | 2011-08-25 | Genisphere, Llc | DNA Dendrimers as Thermal Ablation Devices |
| US20130035259A1 (en) | 2010-11-22 | 2013-02-07 | The University Of Chicago | Methods and/or Systems Producing and Providing Sets of Oligonucleotide Conjugates for Assays and Detections |
| WO2013177046A1 (en) | 2012-05-21 | 2013-11-28 | Solulink, Inc. | Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization |
| US9651549B2 (en) | 2012-07-13 | 2017-05-16 | Genisphere, Llc | Lateral flow assays using DNA dendrimers |
| WO2014153394A1 (en) | 2013-03-21 | 2014-09-25 | Genisphere, Llc | Cellular delivery of dna intercalating agents |
| JP7366541B2 (ja) | 2016-02-19 | 2023-10-23 | コード バイオセラピューティクス インコーポレイテッド | 核酸キャリア及び治療上の使用方法 |
| WO2017143171A1 (en) * | 2016-02-19 | 2017-08-24 | Genisphere Llc | Nucleic acid carriers and therapeutic methods of use |
| EP4215620A4 (en) * | 2020-09-17 | 2024-10-23 | Yokogawa Electric Corporation | NUCLEIC ACID SEQUENCE MEASURING METHOD AND NUCLEIC ACID SEQUENCE MEASURING KIT |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5288609A (en) * | 1984-04-27 | 1994-02-22 | Enzo Diagnostics, Inc. | Capture sandwich hybridization method and composition |
| US5175270A (en) * | 1986-09-10 | 1992-12-29 | Polyprobe, Inc. | Reagents for detecting and assaying nucleic acid sequences |
| CA2039517C (en) * | 1990-04-03 | 2006-11-07 | David Segev | Dna probe signal amplification |
| US5561043A (en) * | 1994-01-31 | 1996-10-01 | Trustees Of Boston University | Self-assembling multimeric nucleic acid constructs |
-
1998
- 1998-07-29 DE DE69834645T patent/DE69834645T2/de not_active Expired - Lifetime
- 1998-07-29 EP EP98937269A patent/EP1005573B1/en not_active Expired - Lifetime
- 1998-07-29 AU AU86018/98A patent/AU8601898A/en not_active Abandoned
- 1998-07-29 WO PCT/US1998/015764 patent/WO1999006595A1/en not_active Ceased
- 1998-07-29 JP JP2000505334A patent/JP4221145B2/ja not_active Expired - Lifetime
- 1998-07-29 AT AT98937269T patent/ATE327344T1/de not_active IP Right Cessation
- 1998-07-29 ES ES98937269T patent/ES2270527T3/es not_active Expired - Lifetime
-
2000
- 2000-01-27 US US09/492,471 patent/US6274723B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1005573B1 (en) | 2006-05-24 |
| AU8601898A (en) | 1999-02-22 |
| DE69834645D1 (de) | 2006-06-29 |
| JP4221145B2 (ja) | 2009-02-12 |
| US6274723B1 (en) | 2001-08-14 |
| JP2001512034A (ja) | 2001-08-21 |
| EP1005573A1 (en) | 2000-06-07 |
| EP1005573A4 (en) | 2004-11-10 |
| ES2270527T3 (es) | 2007-04-01 |
| ATE327344T1 (de) | 2006-06-15 |
| WO1999006595A1 (en) | 1999-02-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69834645T2 (de) | Dendritische nukleinsäuren mit maximaler selbstzusammenlagerung | |
| DE69736667T2 (de) | Verfahren zum nachweis und amplifikation von nukleinsäuresequenzen unter verbrauch von modifizierten oligonukleotiden mit erhöhter zielschmelztemperatur (tm) | |
| DE69334173T2 (de) | Verfahren zum Nachweis einer Zielnukleinsäure | |
| DE60208278T2 (de) | System und Verfahren zum Testen von Nukleinsäuremolekülen | |
| DE60223276T2 (de) | Verfahren zur Blockierung von unspezifischen Hybridisierungen von Nukleinsäuresequenzen | |
| DE69930379T2 (de) | Oligonukleotide mit pyrazolä3,4-dü pyrimidin zur hybridisierung und unterscheidung von fehlpaarungen | |
| DE60133111T2 (de) | Verfahren zur erkennung und zum assay von nukleinsäuresequenzen | |
| DE68923603T2 (de) | Nukleotid Sonden. | |
| EP1230398B1 (de) | Farbstoffmarkiertes oligonukleotid zum markieren eines nukleinsäuremoleküls | |
| WO2018114674A1 (de) | Zweiteilige mediatorsonde | |
| DE60027322T2 (de) | Verfahren zur erkennung von zielnukleinsauren | |
| DE60133321T2 (de) | Methoden zur Detektion des mecA Gens beim methicillin-resistenten Staphylococcus Aureus | |
| DE102011056606B3 (de) | Verfahren zur elektrochemischen Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen | |
| EP0962536A1 (de) | DNA-Nachweis über einen Strangreassoziationskomplex | |
| DE102009044795B3 (de) | Kompetitionsassay zur Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen | |
| DE69728370T2 (de) | Analyseverfahren basierend auf Signalverstärkung | |
| DE60029607T2 (de) | Elektrochemilumineszenz-helikasetest | |
| DE19858588B4 (de) | Farbstoffmarkiertes Oligonukleotid zum Markieren eines Nukleinsäuremoleküls | |
| DE102012203964B3 (de) | Verfahren und Kit zur Detektion von Nukleinsäuren | |
| DE112016007245T5 (de) | Verfahren zum Nachweisen von Ziel-Nukleinsäuremolekülen | |
| WO2000032810A1 (de) | In situ-hybridisierungsverfahren mit sonden, welche dendrimerstrukturen enthalten | |
| DE19952568C2 (de) | Fluoreszenzmarkierte Nukleinsäure-Liganden | |
| US20240052414A1 (en) | Nucleic acid nanostructures | |
| WO2006013052A1 (de) | Verfahren zum analysieren von proben mittels einer hybridisierung | |
| WO2003040679A2 (de) | Reversible bindung eines fluorophors an eine oberfläche zur detektion von ligat-ligand-assoziationsereingnissen durch fluoreszenz-quenchen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: GENISPHERE INC., HATFIELD, PA., US |
|
| 8327 | Change in the person/name/address of the patent owner |
Owner name: GENISPHERE, LLC., HATFIELD, PA., US |