DE69818458T3 - Ribosom-komplexe als selektionspartikel für die in vitro anzeige und evolution von proteinen - Google Patents
Ribosom-komplexe als selektionspartikel für die in vitro anzeige und evolution von proteinen Download PDFInfo
- Publication number
- DE69818458T3 DE69818458T3 DE69818458T DE69818458T DE69818458T3 DE 69818458 T3 DE69818458 T3 DE 69818458T3 DE 69818458 T DE69818458 T DE 69818458T DE 69818458 T DE69818458 T DE 69818458T DE 69818458 T3 DE69818458 T3 DE 69818458T3
- Authority
- DE
- Germany
- Prior art keywords
- mrna
- protein
- peptide
- dna
- pcr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9710829 | 1997-05-28 | ||
| GBGB9710829.4A GB9710829D0 (en) | 1997-05-28 | 1997-05-28 | Selection particles for in vitro display and evolution of antibody combining sites |
| GBGB9724850.4A GB9724850D0 (en) | 1997-11-26 | 1997-11-26 | Selection particles for in vitro display and evolution of antibody combining sites |
| GB9724850 | 1997-11-26 | ||
| GBGB9804195.7A GB9804195D0 (en) | 1998-02-28 | 1998-02-28 | Selection particles for in vitro display and evolution of antibody combining sites |
| GB9804195 | 1998-02-28 | ||
| PCT/GB1998/001564 WO1998054312A1 (en) | 1997-05-28 | 1998-05-28 | Ribosome complexes as selection particles for in vitro display and evolution of proteins |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DE69818458D1 DE69818458D1 (de) | 2003-10-30 |
| DE69818458T2 DE69818458T2 (de) | 2004-07-08 |
| DE69818458T3 true DE69818458T3 (de) | 2009-12-17 |
Family
ID=27268863
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69818458T Expired - Lifetime DE69818458T3 (de) | 1997-05-28 | 1998-05-28 | Ribosom-komplexe als selektionspartikel für die in vitro anzeige und evolution von proteinen |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6620587B1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP0985032B2 (cg-RX-API-DMAC7.html) |
| JP (1) | JP4060892B2 (cg-RX-API-DMAC7.html) |
| AT (1) | ATE250668T1 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2291721C (cg-RX-API-DMAC7.html) |
| DE (1) | DE69818458T3 (cg-RX-API-DMAC7.html) |
| DK (1) | DK0985032T4 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2209145T5 (cg-RX-API-DMAC7.html) |
| PT (1) | PT985032E (cg-RX-API-DMAC7.html) |
| WO (1) | WO1998054312A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (76)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU4854999A (en) * | 1998-07-02 | 2000-01-24 | Invitro Diagnostics, Inc. | Methods, compositions and apparatus for making nucleic acid molecules having a selected affinity to a target molecule |
| DE19932688B4 (de) | 1999-07-13 | 2009-10-08 | Scil Proteins Gmbh | Design von Beta-Faltblatt-Proteinen des gamma-II-kristallins antikörperähnlichen |
| GB9917027D0 (en) | 1999-07-20 | 1999-09-22 | Affibody Technology Sweeden Ab | In vitro selection and optional identification of polypeptides using solid support carriers |
| IL150291A0 (en) * | 2000-01-11 | 2002-12-01 | Maxygen Inc | Integrated systems and methods for diversity generation and screening |
| EP1652919A1 (en) * | 2000-03-31 | 2006-05-03 | Cambridge Antibody Technology LTD | Improvements to ribosome display |
| CA2402452A1 (en) * | 2000-03-31 | 2001-10-11 | Cambridge Antibody Technology Limited | Improvements to ribosome display |
| US20020098524A1 (en) | 2000-04-14 | 2002-07-25 | Murray Christopher J. | Methods for selective targeting |
| US7129326B2 (en) | 2000-04-14 | 2006-10-31 | Genencor International, Inc. | Methods for selective targeting |
| EP1372721A4 (en) | 2001-03-07 | 2008-04-02 | Childrens Medical Center | METHOD FOR SCREENING PEPTIDE DISPLAY LIBRARIES WITH A MINIMUM DISPLAY |
| US20060234231A1 (en) * | 2001-06-20 | 2006-10-19 | Nuevolution A/S | Microarrays displaying encoded molecules |
| EP1401850A1 (en) | 2001-06-20 | 2004-03-31 | Nuevolution A/S | Nucleoside derivatives for library preparation |
| DE10145226A1 (de) * | 2001-09-13 | 2003-04-10 | Lifebits Ag | Herstellung von trägergebundenen Molekülen |
| DE10147074A1 (de) * | 2001-09-25 | 2003-05-08 | Beru Ag | Verfahren zum Betreiben einer aus mehreren Heizelementen bestehenden mehrstufigen elektrischen Heizung |
| DE20200926U1 (de) * | 2002-01-23 | 2002-04-18 | Hegenscheidt-MFD GmbH & Co. KG, 41812 Erkelenz | Festwalzgerät einer Festwalzmaschine für Kurbelwellen |
| US10731151B2 (en) | 2002-03-15 | 2020-08-04 | Nuevolution A/S | Method for synthesising templated molecules |
| US20050247001A1 (en) * | 2002-03-15 | 2005-11-10 | Nuevolution A/S | Building block forming a c-c or a c-hetero atom bond uponreaction |
| AU2003214031A1 (en) | 2002-03-15 | 2003-09-29 | Nuevolution A/S | An improved method for synthesising templated molecules |
| WO2003078050A2 (en) * | 2002-03-15 | 2003-09-25 | Nuevolution A/S | A building block forming a c-c bond upon reaction |
| WO2003078627A2 (en) * | 2002-03-15 | 2003-09-25 | Nuevolution A/S | A building block capable of functional entity transfer to nucleophil |
| EP1539980B1 (en) * | 2002-08-01 | 2016-02-17 | Nuevolution A/S | Library of complexes comprising small non-peptide molecules and double-stranded oligonucleotides identifying the molecules |
| DK1534830T3 (da) * | 2002-09-06 | 2011-02-14 | Isogenica Ltd | In vito-peptid-ekspressionsbibliotek |
| EP2348124B1 (en) * | 2002-10-30 | 2013-12-11 | Nuevolution A/S | Synthesis of a bifunctional complex |
| EP2581383A1 (en) | 2002-11-25 | 2013-04-17 | Genencor International, Inc. | Skin or hair binding peptides |
| WO2004056994A2 (en) * | 2002-12-19 | 2004-07-08 | Nuevolution A/S | Quasirandom structure and function guided synthesis methods |
| US20070026397A1 (en) | 2003-02-21 | 2007-02-01 | Nuevolution A/S | Method for producing second-generation library |
| WO2004074501A2 (en) * | 2003-02-21 | 2004-09-02 | Nuevolution A/S | A method for obtaining structural information about an encoded molecule |
| ES2326649T3 (es) * | 2003-03-19 | 2009-10-16 | Crescendo Biologics Ltd. | Recuperacion de dna. |
| US7915201B2 (en) * | 2003-03-20 | 2011-03-29 | Nuevolution A/S | Ligational encoding of small molecules |
| DE10324447A1 (de) | 2003-05-28 | 2004-12-30 | Scil Proteins Gmbh | Generierung künstlicher Bindungsproteine auf der Grundlage von Ubiquitin |
| GB0316314D0 (en) * | 2003-07-12 | 2003-08-13 | Univ Belfast | Method |
| US11118215B2 (en) * | 2003-09-18 | 2021-09-14 | Nuevolution A/S | Method for obtaining structural information concerning an encoded molecule and method for selecting compounds |
| WO2005078122A2 (en) * | 2004-02-17 | 2005-08-25 | Nuevolution A/S | Method for enrichment involving elimination by mismatch hybridisation |
| DE602005017370D1 (de) * | 2004-03-22 | 2009-12-10 | Nuevolution As | Ligationscodierung unter verwendung von oligonukleotidbausteinen |
| US20050250112A1 (en) * | 2004-05-07 | 2005-11-10 | Padmabandu Gothami A | Nucleic acids, compositions, methods, and kits for detecting mycoplasma and acholeplasma species |
| US9006393B1 (en) | 2004-08-26 | 2015-04-14 | Patricia L. Clark | Molecular constructs and uses thereof in ribosomal translational events |
| DE102004049479A1 (de) | 2004-10-11 | 2006-04-13 | Scil Proteins Gmbh | Proteinkonjugate zur Verwendung in Therapie, Diagnose und Chromatographie |
| PT3305900T (pt) | 2005-12-01 | 2021-10-27 | Nuevolution As | Métodos de codificação enzimática para síntese eficaz de bibliotecas grandes |
| GB0605584D0 (en) | 2006-03-20 | 2006-04-26 | Olink Ab | Method for analyte detection using proximity probes |
| US7923533B2 (en) * | 2006-06-29 | 2011-04-12 | The Invention Science Fund I, Llc | Methods for arbitrary peptide synthesis |
| US7910695B2 (en) | 2006-06-29 | 2011-03-22 | The Invention Science Fund I, Llc | Methods for arbitrary peptide synthesis |
| US7879975B2 (en) * | 2006-06-29 | 2011-02-01 | The Invention Science Fund I, Llc | Methods for arbitrary peptide synthesis |
| US7993873B2 (en) * | 2006-06-29 | 2011-08-09 | The Invention Science Fund I, Llc | Apparatus for arbitrary peptide synthesis |
| US7777002B2 (en) * | 2006-06-29 | 2010-08-17 | The Invention Science Fund I, Llc | Methods for arbitrary peptide synthesis |
| EP1925664A1 (en) | 2006-11-15 | 2008-05-28 | Scil proteins GmbH | Artificial binding proteins based on a modified alpha helical region of ubiquitin |
| US8865179B2 (en) * | 2008-01-26 | 2014-10-21 | Swey-Shen Alexchen | Aptameric IgE peptides in a protein scaffold as an allergy vaccine |
| EP2316030B1 (en) | 2008-07-25 | 2019-08-21 | Wagner, Richard W. | Protein screeing methods |
| KR20110076906A (ko) * | 2008-09-30 | 2011-07-06 | 아보트 러보러터리즈 | 개선된 rna 디스플레이 방법 |
| RU2553333C2 (ru) | 2009-12-14 | 2015-06-10 | Сцил Протеинс Гмбх | Способ идентификации гетеромультимерных модифицированных убиквитиновых белков со способностью связываться с лигандами |
| WO2011111832A1 (ja) | 2010-03-11 | 2011-09-15 | 独立行政法人理化学研究所 | ポリペプチド配列の選択方法、並びに金属酸化物または含珪素化合物結合ペプチドおよびその利用 |
| GB201004292D0 (en) | 2010-03-15 | 2010-04-28 | Olink Ab | Assay for localised detection of analytes |
| EP3540059A1 (en) | 2010-04-16 | 2019-09-18 | Nuevolution A/S | Bi-functional complexes and methods for making and using such complexes |
| GB201011971D0 (en) | 2010-07-15 | 2010-09-01 | Olink Ab | Methods and product |
| US10669569B2 (en) | 2010-10-15 | 2020-06-02 | Navinci Diagnostics Ab | Dynamic range methods |
| EP2466310A1 (en) | 2010-12-17 | 2012-06-20 | Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt GmbH | Means and methods for detecting double-strand breaks |
| DE102010056289A1 (de) | 2010-12-24 | 2012-06-28 | Geneart Ag | Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken |
| GB201101621D0 (en) | 2011-01-31 | 2011-03-16 | Olink Ab | Method and product |
| JP5948673B2 (ja) | 2011-03-10 | 2016-07-06 | ジーンフロンティア株式会社 | リポ多糖混入量を低減した蛋白質合成用組成物、該組成物を用いた蛋白質製造方法 |
| GB201107863D0 (en) | 2011-05-11 | 2011-06-22 | Olink Ab | Method and product |
| JP6057297B2 (ja) | 2011-05-23 | 2017-01-11 | 国立研究開発法人理化学研究所 | 核酸構築物、核酸−蛋白質複合体、及びその利用 |
| CA2837804C (en) | 2011-06-15 | 2018-03-20 | Scil Proteins Gmbh | Dimeric binding proteins based on modified ubiquitins |
| GB201201547D0 (en) | 2012-01-30 | 2012-03-14 | Olink Ab | Method and product |
| WO2015120058A2 (en) | 2014-02-05 | 2015-08-13 | Molecular Templates, Inc. | Methods of screening, selecting, and identifying cytotoxic recombinant polypeptides based on an interim diminution of ribotoxicity |
| EP3209776A1 (en) * | 2014-10-22 | 2017-08-30 | Crescendo Biologics Limited | Human vh domain scaffolds |
| JP6738340B2 (ja) | 2015-02-06 | 2020-08-12 | ナフィゴ プロテインズ ゲゼルシャフト ミット ベシュレンクテル ハフツングNavigo Proteins GmbH | 新規なegfr結合タンパク質 |
| JP6856938B2 (ja) | 2015-07-16 | 2021-04-14 | ナフィゴ プロテインズ ゲゼルシャフト ミット ベシュレンクテル ハフツングNavigo Proteins GmbH | 新規な免疫グロブリン結合タンパク質およびアフィニティ精製におけるそれらの使用 |
| WO2017013136A1 (en) | 2015-07-20 | 2017-01-26 | Scil Proteins Gmbh | Novel binding proteins based on di-ubiquitin muteins and methods for generation |
| MX2018003532A (es) | 2015-09-24 | 2019-04-25 | Abvitro Llc | Conjugados de afinidad-oligonucleotido y usos de los mismos. |
| US11813336B2 (en) | 2016-05-04 | 2023-11-14 | Navigo Proteins Gmbh | Targeted compounds for the site-specific coupling of chemical moieties comprising a peptide linker |
| CN109963864B (zh) | 2016-08-11 | 2024-01-02 | 瑞普利金公司 | 用于亲和色谱的碱性稳定性fc结合蛋白 |
| JP6929354B2 (ja) | 2016-09-24 | 2021-09-01 | アブビトロ, エルエルシー | 親和性−オリゴヌクレオチドコンジュゲートおよびその使用 |
| US11414466B2 (en) | 2017-11-07 | 2022-08-16 | Navigo Proteins Gmbh | Fusion proteins with specificity for ED-B and long serum half-life for diagnosis or treatment of cancer |
| MX2021007283A (es) | 2018-12-18 | 2021-07-15 | Navigo Proteins Gmbh | Proteinas de union especificas del folr1 novedosas para diagnostico y tratamiento del cancer. |
| MX2022001664A (es) | 2019-08-09 | 2022-07-19 | Nutcracker Therapeutics Inc | Aparato microfluídico y metodos de uso del mismo. |
| EP4382534A1 (en) | 2022-12-06 | 2024-06-12 | Biomérieux | Synthetic peptide for use in an immunomolecular assay |
| EP4655590A1 (en) | 2023-01-27 | 2025-12-03 | Biomerieux | Modified immuno-molecular assay for faster biomarker detection |
| WO2025111396A1 (en) | 2023-11-20 | 2025-05-30 | Alamar Biosciences, Inc. | Molecular barcode set and use thereof in multiplex proximity detection assays |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06178688A (ja) | 1992-12-11 | 1994-06-28 | Mitsui Toatsu Chem Inc | E型緑膿菌を抗原とするヒト抗体をコードする遺伝子 |
| WO1995011922A1 (en) | 1993-10-29 | 1995-05-04 | Affymax Technologies N.V. | In vitro peptide and antibody display libraries |
| CA2402452A1 (en) | 2000-03-31 | 2001-10-11 | Cambridge Antibody Technology Limited | Improvements to ribosome display |
-
1998
- 1998-05-28 WO PCT/GB1998/001564 patent/WO1998054312A1/en not_active Ceased
- 1998-05-28 JP JP50038699A patent/JP4060892B2/ja not_active Expired - Fee Related
- 1998-05-28 AT AT98924468T patent/ATE250668T1/de active
- 1998-05-28 EP EP98924468A patent/EP0985032B2/en not_active Expired - Lifetime
- 1998-05-28 US US09/424,712 patent/US6620587B1/en not_active Expired - Lifetime
- 1998-05-28 ES ES98924468T patent/ES2209145T5/es not_active Expired - Lifetime
- 1998-05-28 PT PT98924468T patent/PT985032E/pt unknown
- 1998-05-28 DE DE69818458T patent/DE69818458T3/de not_active Expired - Lifetime
- 1998-05-28 CA CA2291721A patent/CA2291721C/en not_active Expired - Fee Related
- 1998-05-28 DK DK98924468T patent/DK0985032T4/da active
Also Published As
| Publication number | Publication date |
|---|---|
| ES2209145T5 (es) | 2009-10-30 |
| AU725957B2 (en) | 2000-10-26 |
| DK0985032T4 (da) | 2009-08-31 |
| JP4060892B2 (ja) | 2008-03-12 |
| CA2291721A1 (en) | 1998-12-03 |
| US6620587B1 (en) | 2003-09-16 |
| WO1998054312A1 (en) | 1998-12-03 |
| DE69818458D1 (de) | 2003-10-30 |
| DK0985032T3 (da) | 2004-02-02 |
| DE69818458T2 (de) | 2004-07-08 |
| EP0985032B2 (en) | 2009-06-03 |
| JP2002500514A (ja) | 2002-01-08 |
| ATE250668T1 (de) | 2003-10-15 |
| EP0985032B1 (en) | 2003-09-24 |
| AU7666698A (en) | 1998-12-30 |
| EP0985032A1 (en) | 2000-03-15 |
| CA2291721C (en) | 2011-08-09 |
| PT985032E (pt) | 2004-02-27 |
| ES2209145T3 (es) | 2004-06-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8363 | Opposition against the patent | ||
| 8366 | Restricted maintained after opposition proceedings | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: CRESCENDO BIOLOGICS LTD., BABRAHAM, CAMBRIDGE, GB |
|
| 8328 | Change in the person/name/address of the agent |
Representative=s name: MARKS & CLERK (LUXEMBOURG) LLP, LUXEMBOURG, LU |